PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24312564-1 2013 Phospholipase C-mediated hydrolysis of phosphatidylinositol 4,5-bisphosphate generates diacylglycerol, inositol 1,4,5-trisphosphate and protons, all of which can regulate TRPV1 activity via different mechanisms. Phosphatidylinositol 4,5-Diphosphate 39-76 transient receptor potential cation channel subfamily V member 1 Homo sapiens 171-176 24158445-5 2013 When we incorporated TRPV1 into planar lipid bilayers consisting of neutral lipids, capsaicin-induced activity depended on phosphatidylinositol 4,5-bisphosphate. Phosphatidylinositol 4,5-Diphosphate 123-160 transient receptor potential cation channel subfamily V member 1 Homo sapiens 21-26 25219274-0 2014 [Functional regulation of TRPV1 by PIP2]. Phosphatidylinositol 4,5-Diphosphate 35-39 transient receptor potential cation channel subfamily V member 1 Homo sapiens 26-31 24798548-2 2014 The anionic phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2) is a known positive modulator of TRPV1 channels and the negatively charged phosphate groups interact with several basic amino acid residues in the proximal C-terminal TRP domain of the TRPV1 channel. Phosphatidylinositol 4,5-Diphosphate 25-62 transient receptor potential cation channel subfamily V member 1 Homo sapiens 103-108 24798548-2 2014 The anionic phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2) is a known positive modulator of TRPV1 channels and the negatively charged phosphate groups interact with several basic amino acid residues in the proximal C-terminal TRP domain of the TRPV1 channel. Phosphatidylinositol 4,5-Diphosphate 25-62 transient receptor potential cation channel subfamily V member 1 Homo sapiens 255-260 24798548-2 2014 The anionic phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2) is a known positive modulator of TRPV1 channels and the negatively charged phosphate groups interact with several basic amino acid residues in the proximal C-terminal TRP domain of the TRPV1 channel. Phosphatidylinositol 4,5-Diphosphate 64-68 transient receptor potential cation channel subfamily V member 1 Homo sapiens 103-108 24798548-2 2014 The anionic phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2) is a known positive modulator of TRPV1 channels and the negatively charged phosphate groups interact with several basic amino acid residues in the proximal C-terminal TRP domain of the TRPV1 channel. Phosphatidylinositol 4,5-Diphosphate 64-68 transient receptor potential cation channel subfamily V member 1 Homo sapiens 255-260 24798548-5 2014 Our results show that LC-CoAs are potent activators of the TRPV1 channel and interact with the same PIP2-binding residues in TRPV1. Phosphatidylinositol 4,5-Diphosphate 100-104 transient receptor potential cation channel subfamily V member 1 Homo sapiens 125-130 23074220-5 2012 In addition, recent studies suggested TRPV1 activation via a G(q)-mediated mechanism involving diacylglycerol (DAG) or phosphatidylinositol-4,5-bisphosphate (PIP(2)). Phosphatidylinositol 4,5-Diphosphate 119-156 transient receptor potential cation channel subfamily V member 1 Homo sapiens 38-43 23613529-5 2013 During the last decade the number of endogenous regulators of TRPV1"s activity has increased to include lipids that can negatively regulate TRPV1, as is the case for cholesterol and PIP2 (phosphatidylinositol 4,5-biphosphate) while, in contrast, other lipids produced in response to tissue injury and ischaemic processes are known to positively regulate TRPV1. Phosphatidylinositol 4,5-Diphosphate 182-186 transient receptor potential cation channel subfamily V member 1 Homo sapiens 62-67 23613529-5 2013 During the last decade the number of endogenous regulators of TRPV1"s activity has increased to include lipids that can negatively regulate TRPV1, as is the case for cholesterol and PIP2 (phosphatidylinositol 4,5-biphosphate) while, in contrast, other lipids produced in response to tissue injury and ischaemic processes are known to positively regulate TRPV1. Phosphatidylinositol 4,5-Diphosphate 188-224 transient receptor potential cation channel subfamily V member 1 Homo sapiens 62-67 23439120-1 2013 The capsaicin receptor, TRPV1, is regulated by phosphatidylinositol-4,5-bisphosphate (PIP(2)), although the precise nature of this effect (i.e., positive or negative) remains controversial. Phosphatidylinositol 4,5-Diphosphate 47-84 transient receptor potential cation channel subfamily V member 1 Homo sapiens 4-22 23439120-1 2013 The capsaicin receptor, TRPV1, is regulated by phosphatidylinositol-4,5-bisphosphate (PIP(2)), although the precise nature of this effect (i.e., positive or negative) remains controversial. Phosphatidylinositol 4,5-Diphosphate 47-84 transient receptor potential cation channel subfamily V member 1 Homo sapiens 24-29 23439120-4 2013 Rather, introduction of various phosphoinositides, including PIP(2), PI4P, and phosphatidylinositol, inhibits TRPV1, supporting a model whereby phosphoinositide turnover contributes to thermal hyperalgesia by disinhibiting the channel. Phosphatidylinositol 4,5-Diphosphate 61-67 transient receptor potential cation channel subfamily V member 1 Homo sapiens 110-115 23074220-11 2012 Therefore, we demonstrate dual functional interactions between MRGPR-X1 and TRPV1, resulting in PKC-dependent TRPV1 sensitization and DAG/PIP(2)-mediated activation. Phosphatidylinositol 4,5-Diphosphate 138-144 transient receptor potential cation channel subfamily V member 1 Homo sapiens 76-81 22314297-6 2012 In addition, camphor, which generally is known to decrease the fluidity of cell plasma membranes, may also regulate the activity of TRPV1 by inducing changes in the spatial distribution of phosphatidylinositol-4,5-bisphosphate on the inner leaflet of the plasma membrane. Phosphatidylinositol 4,5-Diphosphate 189-226 transient receptor potential cation channel subfamily V member 1 Homo sapiens 132-137 21224382-0 2011 Localization of the PIP2 sensor of TRPV1 ion channels. Phosphatidylinositol 4,5-Diphosphate 20-24 transient receptor potential cation channel subfamily V member 1 Homo sapiens 35-40 21844219-8 2011 The inhibitory effect of TRPV1 appears to depend on Ca(2+) influx through the activated channel followed by Ca(2+)-sensitive depletion of phosphatidylinositol 4,5-bisphosphate and activation of protein phosphatase calcineurin. Phosphatidylinositol 4,5-Diphosphate 138-175 transient receptor potential cation channel subfamily V member 1 Homo sapiens 25-30 21224382-6 2011 We followed by focusing on the role of the C terminus of TRPV1 in sensing PIP2. Phosphatidylinositol 4,5-Diphosphate 74-78 transient receptor potential cation channel subfamily V member 1 Homo sapiens 57-62 21224382-8 2011 Furthermore, we used a novel in vitro binding assay to demonstrate that the proximal C-terminal region of TRPV1 is sufficient for PIP2 binding. Phosphatidylinositol 4,5-Diphosphate 130-134 transient receptor potential cation channel subfamily V member 1 Homo sapiens 106-111 21224382-9 2011 Together, our data suggest that the proximal C-terminal region of TRPV1 can interact directly with PIP2 and may play a key role in PIP2 regulation of the channel. Phosphatidylinositol 4,5-Diphosphate 99-103 transient receptor potential cation channel subfamily V member 1 Homo sapiens 66-71 19243225-8 2009 These results support a prominent contribution of PIP2 depletion to the desensitization of TRPV1 and suggest the adaptation as a possible physiological function for the Ca(2+) influx through the channel. Phosphatidylinositol 4,5-Diphosphate 50-54 transient receptor potential cation channel subfamily V member 1 Homo sapiens 91-96 18574245-1 2008 Phosphatidylinositol (4,5)-bisphosphate (PI(4,5)P2) is the endogenous lipid regulating TRPV1. Phosphatidylinositol 4,5-Diphosphate 0-39 transient receptor potential cation channel subfamily V member 1 Homo sapiens 87-92 18574245-5 2008 TRPV1 is a good example; although phosphatidylinositol (4,5)-bisphosphate (PI(4,5)P(2)) can potently regulate its activation, we show that phosphatidylinositol (4)-phosphate (PI(4)P) and phosphatidylinositol (3,4,5)-trisphosphate (PI(3,4,5)P(3)) can as well. Phosphatidylinositol 4,5-Diphosphate 34-73 transient receptor potential cation channel subfamily V member 1 Homo sapiens 0-5 18528787-4 2008 Two opposing effects of PIP2 have been reported on TRPV1. Phosphatidylinositol 4,5-Diphosphate 24-28 transient receptor potential cation channel subfamily V member 1 Homo sapiens 51-56 18528787-5 2008 PIP2 has been proposed to inhibit TRPV1, and relief from this inhibition was suggested to be involved in sensitization of these channels by pro-inflammatory agents. Phosphatidylinositol 4,5-Diphosphate 0-4 transient receptor potential cation channel subfamily V member 1 Homo sapiens 34-39 18528787-6 2008 In excised patches, however, PIP2 was shown to activate TRPV1. Phosphatidylinositol 4,5-Diphosphate 29-33 transient receptor potential cation channel subfamily V member 1 Homo sapiens 56-61 18528787-7 2008 Calcium flowing through TRPV1 activates PLC and the resulting depletion of PIP2 was proposed to play a role in capsaicin-induced desensitization of these channels. Phosphatidylinositol 4,5-Diphosphate 75-79 transient receptor potential cation channel subfamily V member 1 Homo sapiens 24-29 18528787-8 2008 We will describe the data indicating involvement of PLC and PIP2 in sensitization and desensitization of TRPV1 and will also discuss other pathways potentially contributing to these two phenomena. Phosphatidylinositol 4,5-Diphosphate 60-64 transient receptor potential cation channel subfamily V member 1 Homo sapiens 105-110 18528787-9 2008 We attempt to resolve the seemingly contradictory data by proposing that PIP2 can both activate and inhibit TRPV1 depending on the experimental conditions, more specifically on the level of stimulation of these channels. Phosphatidylinositol 4,5-Diphosphate 73-77 transient receptor potential cation channel subfamily V member 1 Homo sapiens 108-113 18172555-6 2008 Lidocaine sensitivity of TRPV1 required segments of the putative vanilloid-binding domain within and adjacent to transmembrane domain 3, was diminished under phosphatidylinositol 4,5-bisphosphate depletion, and was abrogated by a point mutation at residue R701 in the proximal C-terminal TRP domain. Phosphatidylinositol 4,5-Diphosphate 158-195 transient receptor potential cation channel subfamily V member 1 Homo sapiens 25-30 15716403-9 2005 The opposite effects of PIP2 on the vanilloid receptor TRPV1 and TRPM8 also implies that the membrane lipid may have dual actions as a bimodal switch to selectively control the heat- and cold-induced responses in nociceptors expressing both channels. Phosphatidylinositol 4,5-Diphosphate 24-28 transient receptor potential cation channel subfamily V member 1 Homo sapiens 55-60 17582331-6 2007 We present a model for the calcium-dependent regulation of TRPV1 via competitive interactions of ATP and calmodulin at the TRPV1-ARD-binding site and discuss its relationship to the C-terminal region previously implicated in interactions with PIP2 and calmodulin. Phosphatidylinositol 4,5-Diphosphate 243-247 transient receptor potential cation channel subfamily V member 1 Homo sapiens 59-64 17074976-2 2006 The currently accepted model is that the NGF-mediated increase in TRPV1 function during hyperalgesia utilizes activation of phospholipase C (PLC) to cleave PIP2, proposed to tonically inhibit TRPV1. Phosphatidylinositol 4,5-Diphosphate 156-160 transient receptor potential cation channel subfamily V member 1 Homo sapiens 66-71 17074976-2 2006 The currently accepted model is that the NGF-mediated increase in TRPV1 function during hyperalgesia utilizes activation of phospholipase C (PLC) to cleave PIP2, proposed to tonically inhibit TRPV1. Phosphatidylinositol 4,5-Diphosphate 156-160 transient receptor potential cation channel subfamily V member 1 Homo sapiens 192-197 17596456-2 2007 There are conflicting reports on the effect of PtdIns(4,5)P2 on transient receptor potential vanilloid 1 (TRPV1) channels. Phosphatidylinositol 4,5-Diphosphate 47-60 transient receptor potential cation channel subfamily V member 1 Homo sapiens 64-104 17596456-2 2007 There are conflicting reports on the effect of PtdIns(4,5)P2 on transient receptor potential vanilloid 1 (TRPV1) channels. Phosphatidylinositol 4,5-Diphosphate 47-60 transient receptor potential cation channel subfamily V member 1 Homo sapiens 106-111 17596456-3 2007 We show that in excised patches PtdIns(4,5)P2 and other phosphoinositides activate and the PIP2 scavenger poly-Lys inhibits TRPV1. Phosphatidylinositol 4,5-Diphosphate 91-95 transient receptor potential cation channel subfamily V member 1 Homo sapiens 124-129 17596456-5 2007 We show that in the presence of extracellular Ca2+, capsaicin activates phospholipase C (PLC) in TRPV1-expressing cells, inducing depletion of both PtdIns(4,5)P2 and its precursor PtdIns(4)P (PIP). Phosphatidylinositol 4,5-Diphosphate 148-161 transient receptor potential cation channel subfamily V member 1 Homo sapiens 97-102 17596456-8 2007 Increasing PtdIns(4,5)P2 levels by coexpressing phosphatidylinositol-4-phosphate 5-kinase inhibited TRPV1 at low but not at saturating capsaicin concentrations. Phosphatidylinositol 4,5-Diphosphate 11-24 transient receptor potential cation channel subfamily V member 1 Homo sapiens 100-105 17596456-9 2007 These data show that at low capsaicin concentrations and other moderate stimuli, PtdIns(4,5)P2 partially inhibits TRPV1 in a cellular context, but this effect is likely to be indirect, because it is not detectable in excised patches. Phosphatidylinositol 4,5-Diphosphate 81-94 transient receptor potential cation channel subfamily V member 1 Homo sapiens 114-119 17548815-4 2007 A chimera in which the proximal part of the C-terminal of TRPV1 replaces an equivalent section of TRPM8 C-terminal is activated by PIP2 and confers the phenotype of heat activation. Phosphatidylinositol 4,5-Diphosphate 131-135 transient receptor potential cation channel subfamily V member 1 Homo sapiens 58-63 15888659-0 2005 Functional recovery from desensitization of vanilloid receptor TRPV1 requires resynthesis of phosphatidylinositol 4,5-bisphosphate. Phosphatidylinositol 4,5-Diphosphate 93-130 transient receptor potential cation channel subfamily V member 1 Homo sapiens 63-68 15888659-9 2005 These data suggest that depletion of PIP2 occurs concomitantly with activation of TRPV1 and its replenishment in the membrane determines recovery of the channel from desensitization. Phosphatidylinositol 4,5-Diphosphate 37-41 transient receptor potential cation channel subfamily V member 1 Homo sapiens 82-87 33766560-3 2021 This model is difficult to reconcile with phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] being a well-established positive regulator of TRPV1. Phosphatidylinositol 4,5-Diphosphate 42-79 transient receptor potential cation channel subfamily V member 1 Homo sapiens 143-148 12764195-0 2003 A modular PIP2 binding site as a determinant of capsaicin receptor sensitivity. Phosphatidylinositol 4,5-Diphosphate 10-14 transient receptor potential cation channel subfamily V member 1 Homo sapiens 48-66 12764195-1 2003 The capsaicin receptor (TRPV1), a heat-activated ion channel of the pain pathway, is sensitized by phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis after phospholipase C activation. Phosphatidylinositol 4,5-Diphosphate 99-136 transient receptor potential cation channel subfamily V member 1 Homo sapiens 4-22 12764195-1 2003 The capsaicin receptor (TRPV1), a heat-activated ion channel of the pain pathway, is sensitized by phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis after phospholipase C activation. Phosphatidylinositol 4,5-Diphosphate 99-136 transient receptor potential cation channel subfamily V member 1 Homo sapiens 24-29 12764195-1 2003 The capsaicin receptor (TRPV1), a heat-activated ion channel of the pain pathway, is sensitized by phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis after phospholipase C activation. Phosphatidylinositol 4,5-Diphosphate 138-142 transient receptor potential cation channel subfamily V member 1 Homo sapiens 4-22 12764195-1 2003 The capsaicin receptor (TRPV1), a heat-activated ion channel of the pain pathway, is sensitized by phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis after phospholipase C activation. Phosphatidylinositol 4,5-Diphosphate 138-142 transient receptor potential cation channel subfamily V member 1 Homo sapiens 24-29 12764195-3 2003 Mutations that weaken PIP2-TRPV1 interaction reduce thresholds for chemical or thermal stimuli, whereas TRPV1 channels in which this region is replaced with a lipid-binding domain from PIP2-activated potassium channels remain inhibited by PIP2. Phosphatidylinositol 4,5-Diphosphate 22-26 transient receptor potential cation channel subfamily V member 1 Homo sapiens 27-32 33766560-3 2021 This model is difficult to reconcile with phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] being a well-established positive regulator of TRPV1. Phosphatidylinositol 4,5-Diphosphate 81-94 transient receptor potential cation channel subfamily V member 1 Homo sapiens 143-148 33766560-4 2021 Here we show that in the presence of PtdIns(4,5)P2 in excised patches, PtdIns, but not PtdIns(4)P, partially inhibited TRPV1 activity at low, but not at high capsaicin concentrations. Phosphatidylinositol 4,5-Diphosphate 37-50 transient receptor potential cation channel subfamily V member 1 Homo sapiens 119-124 33891950-6 2021 Protein kinase C was responsible for AVP-mediated depression of GIRK channels, whereas degradation of phosphatidylinositol 4,5-bisphosphate was involved in V1a receptor-elicited activation of TRPV1 channels. Phosphatidylinositol 4,5-Diphosphate 102-139 transient receptor potential cation channel subfamily V member 1 Homo sapiens 192-197 25425643-0 2015 Molecular determinants of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) binding to transient receptor potential V1 (TRPV1) channels. Phosphatidylinositol 4,5-Diphosphate 26-63 transient receptor potential cation channel subfamily V member 1 Homo sapiens 87-118 25561742-6 2015 In the presence of 2.5 mum phosphatidylinositol 4,5-bisphosphate, TRPV1 channels demonstrated rapid activation at 33-39 C and achieved full channel opening at 42 C. At this temperature range, TRPV1 heat activation exhibited steep temperature dependence (temperature coefficient (Q10) of 18), and the channel openings were accompanied by large changes in entropy and enthalpy, suggesting a substantial conformation change. Phosphatidylinositol 4,5-Diphosphate 27-64 transient receptor potential cation channel subfamily V member 1 Homo sapiens 66-71 25561742-6 2015 In the presence of 2.5 mum phosphatidylinositol 4,5-bisphosphate, TRPV1 channels demonstrated rapid activation at 33-39 C and achieved full channel opening at 42 C. At this temperature range, TRPV1 heat activation exhibited steep temperature dependence (temperature coefficient (Q10) of 18), and the channel openings were accompanied by large changes in entropy and enthalpy, suggesting a substantial conformation change. Phosphatidylinositol 4,5-Diphosphate 27-64 transient receptor potential cation channel subfamily V member 1 Homo sapiens 194-199 32833423-0 2020 Kainic acid activates TRPV1 via a Phospholipase C/PIP2-dependent mechanism in vitro. Phosphatidylinositol 4,5-Diphosphate 50-54 transient receptor potential cation channel subfamily V member 1 Homo sapiens 22-27 32345612-2 2020 Calcium influx through TRPV1 has been shown to activate a calcium-sensitive phospholipase C (PLC) enzyme and to lead to a robust decrease in phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] levels, which is a major contributor to channel desensitization. Phosphatidylinositol 4,5-Diphosphate 141-178 transient receptor potential cation channel subfamily V member 1 Homo sapiens 23-28 25543978-10 2015 Taken together, our data suggest a mechanism for the mutual regulation of PIP2 and the Ca(2+)-binding proteins S100A1 and calmodulin to TRPV1. Phosphatidylinositol 4,5-Diphosphate 74-78 transient receptor potential cation channel subfamily V member 1 Homo sapiens 136-141 25670203-2 2015 We found that activation of TRPV1 channels with capsaicin either in dorsal root ganglion neurons or in a heterologous expression system inhibited the mechanosensitive Piezo1 and Piezo2 channels by depleting phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] and its precursor phosphatidylinositol 4-phosphate [PI(4)P] from the plasma membrane through Ca(2+)-induced phospholipase Cdelta (PLCdelta) activation. Phosphatidylinositol 4,5-Diphosphate 207-244 transient receptor potential cation channel subfamily V member 1 Homo sapiens 28-33 25425643-0 2015 Molecular determinants of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) binding to transient receptor potential V1 (TRPV1) channels. Phosphatidylinositol 4,5-Diphosphate 26-63 transient receptor potential cation channel subfamily V member 1 Homo sapiens 120-125