PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 32687545-1 2020 Interaction of phospholipase D2 (PLD2) with phosphatidylinositol (4,5)-bisphosphate (PIP2) is regarded as the critical step of numerous physiological processes. Phosphatidylinositol 4,5-Diphosphate 44-83 phospholipase D2 Homo sapiens 15-31 32687545-1 2020 Interaction of phospholipase D2 (PLD2) with phosphatidylinositol (4,5)-bisphosphate (PIP2) is regarded as the critical step of numerous physiological processes. Phosphatidylinositol 4,5-Diphosphate 44-83 phospholipase D2 Homo sapiens 33-37 32687545-1 2020 Interaction of phospholipase D2 (PLD2) with phosphatidylinositol (4,5)-bisphosphate (PIP2) is regarded as the critical step of numerous physiological processes. Phosphatidylinositol 4,5-Diphosphate 85-89 phospholipase D2 Homo sapiens 15-31 32687545-1 2020 Interaction of phospholipase D2 (PLD2) with phosphatidylinositol (4,5)-bisphosphate (PIP2) is regarded as the critical step of numerous physiological processes. Phosphatidylinositol 4,5-Diphosphate 85-89 phospholipase D2 Homo sapiens 33-37 10413092-3 1999 Intriguingly, phosphatidylinositol 4,5-bisphosphate (PIP2) synergistically stimulates the oleate-dependent PLD2 activity with an optimal concentration of 2.5 microM. Phosphatidylinositol 4,5-Diphosphate 14-51 phospholipase D2 Homo sapiens 107-111 25308783-9 2015 These consist of R236/W238 and R557/W563 and a novel PIP2 binding site in the PH domain of PLD2, specifically R210/R212/W233. Phosphatidylinositol 4,5-Diphosphate 53-57 phospholipase D2 Homo sapiens 91-95 15976455-9 2005 It is likely that this phospholipase D2-generated phosphatidate directly stimulates phosphatidylinositol 4-phosphate 5-kinase Igamma b to generate phosphatidylinositol 4,5-bisphosphate as this mechanism has previously been demonstrated in vitro. Phosphatidylinositol 4,5-Diphosphate 147-184 phospholipase D2 Homo sapiens 23-39 15976455-10 2005 Thus, our data indicates that during the initial stages of adhesion, phospholipase D2-derived phosphatidate stimulates phosphatidylinositol 4-phosphate 5-kinase Igamma b to generate phosphatidylinositol 4,5-bisphosphate and that consequently this inositol phospholipid promotes adhesion through its regulation of cell-surface integrins. Phosphatidylinositol 4,5-Diphosphate 182-219 phospholipase D2 Homo sapiens 69-85 11706993-3 2001 Two mammalian PLD enzymes (PLD1 and PLD2) have been cloned and their intracellular regulators identified as ARF and Rho proteins, protein kinase Calpha as well as the lipid, phosphatidylinositol bisphosphate (PIP2). Phosphatidylinositol 4,5-Diphosphate 209-213 phospholipase D2 Homo sapiens 36-40 10656969-0 2000 Inhibition of phosphatidylinositol 4,5-bisphosphate-stimulated phospholipase D2 activity by Ser/Thr phosphorylation. Phosphatidylinositol 4,5-Diphosphate 14-51 phospholipase D2 Homo sapiens 63-79 10656969-1 2000 Treatment of HeLa cells overexpressing PLD2 with the Ser/Thr-specific protein phosphatase inhibitor, okadaic acid, augmented spontaneous phosphorylation of PLD2 with concomitant inhibition of phosphatidylinositol 4,5-bisphosphate (PIP(2))-stimulated PLD2 activity. Phosphatidylinositol 4,5-Diphosphate 192-229 phospholipase D2 Homo sapiens 39-43 10656969-1 2000 Treatment of HeLa cells overexpressing PLD2 with the Ser/Thr-specific protein phosphatase inhibitor, okadaic acid, augmented spontaneous phosphorylation of PLD2 with concomitant inhibition of phosphatidylinositol 4,5-bisphosphate (PIP(2))-stimulated PLD2 activity. Phosphatidylinositol 4,5-Diphosphate 231-238 phospholipase D2 Homo sapiens 39-43 10656969-1 2000 Treatment of HeLa cells overexpressing PLD2 with the Ser/Thr-specific protein phosphatase inhibitor, okadaic acid, augmented spontaneous phosphorylation of PLD2 with concomitant inhibition of phosphatidylinositol 4,5-bisphosphate (PIP(2))-stimulated PLD2 activity. Phosphatidylinositol 4,5-Diphosphate 231-238 phospholipase D2 Homo sapiens 156-160 10656969-1 2000 Treatment of HeLa cells overexpressing PLD2 with the Ser/Thr-specific protein phosphatase inhibitor, okadaic acid, augmented spontaneous phosphorylation of PLD2 with concomitant inhibition of phosphatidylinositol 4,5-bisphosphate (PIP(2))-stimulated PLD2 activity. Phosphatidylinositol 4,5-Diphosphate 231-238 phospholipase D2 Homo sapiens 156-160 25532944-6 2015 The mechanism of action of FIPI is a direct effect on the catalytic site (and as such inhibits both PLD1 and PLD2 isoforms), whereas PLD2 affects both the catalytic site (orthosteric) and blocks PIP2 binding to PLD2 (allosteric), which negates the natural enhancing role of PIP2. Phosphatidylinositol 4,5-Diphosphate 195-199 phospholipase D2 Homo sapiens 133-137 25532944-6 2015 The mechanism of action of FIPI is a direct effect on the catalytic site (and as such inhibits both PLD1 and PLD2 isoforms), whereas PLD2 affects both the catalytic site (orthosteric) and blocks PIP2 binding to PLD2 (allosteric), which negates the natural enhancing role of PIP2. Phosphatidylinositol 4,5-Diphosphate 195-199 phospholipase D2 Homo sapiens 133-137 21930784-7 2011 WASp association to PLD2-K758R, a lipase-inactive mutant, still occurs, albeit at lower levels, indicating that PLD2 plays a second role in phagocytosis, which is the production of phosphatidic acid (PA) and activation of phosphatidylinositol 5-kinase (PI5K) with subsequent synthesis of phosphatidylinositol 4,5-bisphosphate (PIP(2)). Phosphatidylinositol 4,5-Diphosphate 288-325 phospholipase D2 Homo sapiens 20-24 21930784-7 2011 WASp association to PLD2-K758R, a lipase-inactive mutant, still occurs, albeit at lower levels, indicating that PLD2 plays a second role in phagocytosis, which is the production of phosphatidic acid (PA) and activation of phosphatidylinositol 5-kinase (PI5K) with subsequent synthesis of phosphatidylinositol 4,5-bisphosphate (PIP(2)). Phosphatidylinositol 4,5-Diphosphate 288-325 phospholipase D2 Homo sapiens 112-116 11551222-6 2001 Partially purified PLD2 exhibited an absolute requirement for PIP2 when the activity was measured using Triton X-100 micelles. Phosphatidylinositol 4,5-Diphosphate 62-66 phospholipase D2 Homo sapiens 19-23 11551222-7 2001 Ceramides inhibited PLD2 activity, and this inhibition was decreased as PIP2 concentrations increased. Phosphatidylinositol 4,5-Diphosphate 72-76 phospholipase D2 Homo sapiens 20-24 11551222-8 2001 However, C2-ceramide also reversibly inhibited the activity of PLD1 and PLD2 mutants in which binding of PIP2 was decreased, indicating that ceramides are interacting with the catalytic core of the mammalian PLDs. Phosphatidylinositol 4,5-Diphosphate 105-109 phospholipase D2 Homo sapiens 72-76 11551222-10 2001 Our results provide a novel demonstration that ceramides reversibly inhibit mammalian PLD2 as well as PLD1 activities and that both of these actions are more pronounced when PIP2 concentrations are rate-limiting. Phosphatidylinositol 4,5-Diphosphate 174-178 phospholipase D2 Homo sapiens 86-90 11032811-0 2000 Interaction of the type Ialpha PIPkinase with phospholipase D: a role for the local generation of phosphatidylinositol 4, 5-bisphosphate in the regulation of PLD2 activity. Phosphatidylinositol 4,5-Diphosphate 98-136 phospholipase D2 Homo sapiens 158-162 10413092-3 1999 Intriguingly, phosphatidylinositol 4,5-bisphosphate (PIP2) synergistically stimulates the oleate-dependent PLD2 activity with an optimal concentration of 2.5 microM. Phosphatidylinositol 4,5-Diphosphate 53-57 phospholipase D2 Homo sapiens 107-111 10413092-4 1999 These results provide the first evidence that oleate is a PLD2-specific activating factor and PLD2 activity is synergistically stimulated by oleate and PIP2. Phosphatidylinositol 4,5-Diphosphate 152-156 phospholipase D2 Homo sapiens 58-62 10413092-4 1999 These results provide the first evidence that oleate is a PLD2-specific activating factor and PLD2 activity is synergistically stimulated by oleate and PIP2. Phosphatidylinositol 4,5-Diphosphate 152-156 phospholipase D2 Homo sapiens 94-98 9582313-13 1998 hPLD1 and hPLD2 both showed a requirement for phosphatidylinositol 4,5-bisphosphate. Phosphatidylinositol 4,5-Diphosphate 46-83 phospholipase D2 Homo sapiens 10-15 9582313-16 1998 Thus, not only PLD1 but also hPLD2 activity can be positively regulated by both phosphatidylinositol 4,5-bisphosphate and ARF. Phosphatidylinositol 4,5-Diphosphate 80-117 phospholipase D2 Homo sapiens 29-34 9207251-2 1997 Two mammalian PLDs were recently cloned: PLD1, which is activated by Arf family GTPases and dependent upon phosphatidylinositol-4,5-bisphosphate (PIP2), and PLD2, which is also dependent upon PIP2, but not stimulated by Arf. Phosphatidylinositol 4,5-Diphosphate 192-196 phospholipase D2 Homo sapiens 157-161