PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9130007-8 1997 The LPS and IFN-gamma induced CB increases were abolished by cycloheximide or actinomycin D in the cultures, indicating that the increases in CB required increased RNA transcription and de novo protein synthesis. Dactinomycin 78-91 interferon gamma Homo sapiens 12-21 9734473-5 1998 Actinomycin D chase by Northern blotting showed that lyso-PC significantly prolonged IFN-gamma mRNA half-lives in human T cells. Dactinomycin 0-13 interferon gamma Homo sapiens 85-94 9670962-7 1998 T4 increased IFN-gamma-induced HLA-DR protein 2.2-fold and HLA-DR mRNA fourfold after 2 d. Treatment with actinomycin D after induction of HLA-DR mRNA with IFN-gamma, with or without T4, showed that thyroid hormone decreased the t(1/2) of mRNA from 2.4 to 1.1 h. HeLa and CV-1 cells lack functional nuclear thyroid hormone receptor. Dactinomycin 106-119 interferon gamma Homo sapiens 13-22 9670962-7 1998 T4 increased IFN-gamma-induced HLA-DR protein 2.2-fold and HLA-DR mRNA fourfold after 2 d. Treatment with actinomycin D after induction of HLA-DR mRNA with IFN-gamma, with or without T4, showed that thyroid hormone decreased the t(1/2) of mRNA from 2.4 to 1.1 h. HeLa and CV-1 cells lack functional nuclear thyroid hormone receptor. Dactinomycin 106-119 interferon gamma Homo sapiens 156-165 9671113-3 1998 The levels of serotonin transporter mRNA were increased by treatment with interferon-alpha and -gamma for 3 h. The increase in serotonin transporter mRNA elicited by the interferons was inhibited by treatment with actinomycin D, an inhibitor of transcription. Dactinomycin 214-227 interferon gamma Homo sapiens 74-101 9142139-7 1997 The appearance of IFN-gamma at late stages of HCMV infection and its elimination in the presence of an inhibitor (Actinomycin D) of RNA synthesis indicate a true transcriptional induction of this lymphokine at the RNA and protein levels. Dactinomycin 114-127 interferon gamma Homo sapiens 18-27 9712367-2 1998 The time courses of the inhibition of IFN-gamma-induced kynurenine synthesis by actinomycin D and cycloheximide showed that the indoleamine dioxygenase gene was transcribed as early as 2 h and translated as early as 5 h after initiation of IFN treatment. Dactinomycin 80-93 interferon gamma Homo sapiens 38-47 9754910-4 1998 The ability of IFNgamma to stimulate increased bradykinin receptor expression was abrogated by treatment with either the transcription inhibitor actinomycin D or the protein synthesis inhibitor cycloheximide. Dactinomycin 145-158 interferon gamma Homo sapiens 15-23 9248621-6 1997 This increased expression of IRF-1 mRNA by IFN-gamma was not blocked by treatment with cycloheximide, but was abolished by treatment with actinomycin D. Dactinomycin 138-151 interferon gamma Homo sapiens 43-52 8639901-5 1996 Actinomycin D and 5,6-dichloro-1-beta-ribofuranosylbenzimidazole abolished IFN-gamma-induced C1 INH mRNA expression. Dactinomycin 0-13 interferon gamma Homo sapiens 75-84 8621263-8 1996 Protective effects of IFN-gamma on target cell sensitivity to lysis were blocked by pre-treatment of target cells with actinomycin-D or cycloheximide. Dactinomycin 119-132 interferon gamma Homo sapiens 22-31 8244579-12 1993 To strengthen these findings we evaluated the half-lives of the mRNA after IFN-gamma induction by means of actinomycin D treatment. Dactinomycin 107-120 interferon gamma Homo sapiens 75-84 8176225-8 1994 Pretreatment of monocytes with actinomycin D, however, completely abrogated the effect of IFN-gamma, suggesting a transcriptional regulation. Dactinomycin 31-44 interferon gamma Homo sapiens 90-99 8552275-4 1995 Actinomycin D, inhibited C3 gene induction by IFN-gamma and IL-1 beta suggesting that these cytokines act, in part, at the transcriptional level to enhance C3 expression. Dactinomycin 0-13 interferon gamma Homo sapiens 46-55 7913877-6 1994 When actinomycin D (a protein synthesis inhibitor) was added to the culture medium prior to treatment with IFN gamma and TNF, the LAK sensitivity of SMKT-R-3 recovered to the level demonstrated by the cells that had not received any cytokine treatment. Dactinomycin 5-18 interferon gamma Homo sapiens 107-116 8502244-4 1993 The addition of actinomycin D (ActD), a transcriptional inhibitor, together with either PMA or IFN-gamma diminishes the enhanced levels of CD32C mRNA to the basal levels, indicating that transcriptional regulation is involved in this modulatory process. Dactinomycin 16-29 interferon gamma Homo sapiens 95-104 7680009-4 1993 Actinomycin D abolished the IFN gamma- and TNF alpha-induced increases in iNOS mRNA and nitrite production. Dactinomycin 0-13 interferon gamma Homo sapiens 28-37 8425199-6 1993 IL-1 caused a time- and dose-dependent increase in 125I-labeled IFN-gamma binding that was maximal at 6 h, persisted for at least 24 h, and was blocked by both actinomycin D and cycloheximide. Dactinomycin 160-173 interferon gamma Homo sapiens 64-73 8425199-8 1993 IL-1 also produced a time- and dose-dependent increase in IFN-gamma receptor mRNA levels that was maximal at 3 h and persisted for at least 24 h. Actinomycin D, but not cycloheximide, completely blocked the IL-1-mediated increase in IFN-gamma receptor mRNA levels. Dactinomycin 146-159 interferon gamma Homo sapiens 58-67 8425199-8 1993 IL-1 also produced a time- and dose-dependent increase in IFN-gamma receptor mRNA levels that was maximal at 3 h and persisted for at least 24 h. Actinomycin D, but not cycloheximide, completely blocked the IL-1-mediated increase in IFN-gamma receptor mRNA levels. Dactinomycin 146-159 interferon gamma Homo sapiens 233-242 1418895-3 1992 Interferon gamma significantly enhanced the cytotoxicity of tumor necrosis factor alpha with dactinomycin on all six cell lines investigated, while in four of six cell lines the cytotoxicity of tumor necrosis factor alpha with doxorubicin was significantly augmented by interferon gamma. Dactinomycin 93-105 interferon gamma Homo sapiens 0-16 2105896-7 1990 Actinomycin D synergistically potentiated the antiproliferative action of IFN gamma. Dactinomycin 0-13 interferon gamma Homo sapiens 74-83 1386713-3 1992 The response of these cell lines to interferon-gamma was measured in two functional assays: a cell proliferation assay and a cell lysis assay after exposure to interferon-gamma with and without actinomycin-D. Dactinomycin 194-207 interferon gamma Homo sapiens 36-52 1386713-6 1992 After treatment with interferon-gamma actinomycin-D, each choriocarcinoma cell line exhibited dose-dependent cell lysis; lysis of Jar was significantly less than that of either BeWo or JEG-3. Dactinomycin 38-51 interferon gamma Homo sapiens 21-37 1901945-5 1991 However, IFN gamma decreased c-fos mRNA stability, as assessed by measuring the half-life of c-fos mRNA in actinomycin D-treated cells. Dactinomycin 107-120 interferon gamma Homo sapiens 9-18 2541209-5 1989 The addition of actinomycin D at the time of virus challenge did not substantially affect the induction of the antiviral state against VSV, but markedly retarded the establishment of IFN-gamma-induced antiviral state against Sindbis virus. Dactinomycin 16-29 interferon gamma Homo sapiens 183-192 2516865-3 1989 Using a combined treatment with cycloheximide and actinomycin D we observed that in HeLa cells IFN-alpha did not need ongoing protein synthesis to induce the enzyme, whereas the addition of cycloheximide prevented the induction by IFN-gamma. Dactinomycin 50-63 interferon gamma Homo sapiens 231-240 2427623-5 1986 The induction of indoleamine 2,3-dioxygenase by IFN-gamma was inhibited by treatment of the cultures with either actinomycin D or cycloheximide, and thus was dependent on both RNA and protein synthesis. Dactinomycin 113-126 interferon gamma Homo sapiens 48-57 3139787-6 1988 However, the functional half-life of IFN-gamma mRNA after actinomycin D treatment was higher in cells that had been treated with PMA when compared with untreated or IL2-treated cells. Dactinomycin 58-71 interferon gamma Homo sapiens 37-46 2452894-8 1988 When cells are treated with IFN-alpha or IFN-gamma in the presence of cycloheximide, and actinomycin D is added prior to the removal of the cycloheximide, the cells produce the IFN-induced 56,000-dalton protein and develop an antiviral state in response to both IFN-alpha and IFN-gamma. Dactinomycin 89-102 interferon gamma Homo sapiens 41-50 2452894-8 1988 When cells are treated with IFN-alpha or IFN-gamma in the presence of cycloheximide, and actinomycin D is added prior to the removal of the cycloheximide, the cells produce the IFN-induced 56,000-dalton protein and develop an antiviral state in response to both IFN-alpha and IFN-gamma. Dactinomycin 89-102 interferon gamma Homo sapiens 276-285 3007500-3 1986 TNF-R expression was significantly increased after 6 h of exposure to IFN-gamma (100 units/ml), and it remained elevated in the continuous presence of IFN-gamma for at least 20 h. Incubation of cells with IFN-gamma in the presence of cycloheximide, followed by treatment with actinomycin D and reversal of the inhibition of protein synthesis, also resulted in increased TNF-R expression as compared to cultures subjected to the same treatments in the absence of IFN-gamma. Dactinomycin 276-289 interferon gamma Homo sapiens 151-160 3007500-3 1986 TNF-R expression was significantly increased after 6 h of exposure to IFN-gamma (100 units/ml), and it remained elevated in the continuous presence of IFN-gamma for at least 20 h. Incubation of cells with IFN-gamma in the presence of cycloheximide, followed by treatment with actinomycin D and reversal of the inhibition of protein synthesis, also resulted in increased TNF-R expression as compared to cultures subjected to the same treatments in the absence of IFN-gamma. Dactinomycin 276-289 interferon gamma Homo sapiens 151-160 3007500-3 1986 TNF-R expression was significantly increased after 6 h of exposure to IFN-gamma (100 units/ml), and it remained elevated in the continuous presence of IFN-gamma for at least 20 h. Incubation of cells with IFN-gamma in the presence of cycloheximide, followed by treatment with actinomycin D and reversal of the inhibition of protein synthesis, also resulted in increased TNF-R expression as compared to cultures subjected to the same treatments in the absence of IFN-gamma. Dactinomycin 276-289 interferon gamma Homo sapiens 151-160 30521981-5 2019 This notion is further supported by Actinomycin D chase experiments showing that IFNgamma-stimulated up-regulation of MLKL is prevented in the presence of the transcriptional inhibitor Actinomycin D. Dactinomycin 36-49 interferon gamma Homo sapiens 81-89 30521981-5 2019 This notion is further supported by Actinomycin D chase experiments showing that IFNgamma-stimulated up-regulation of MLKL is prevented in the presence of the transcriptional inhibitor Actinomycin D. Dactinomycin 185-198 interferon gamma Homo sapiens 81-89 2580063-5 1985 The transferred activity had the characteristics of an IFN-induced antiviral state, in that it was blocked either by actinomycin D or by prevention of cell contact. Dactinomycin 117-130 interferon gamma Homo sapiens 55-58 14769148-8 2003 Using actinomycin D as a transcriptional inhibitor, we show that the mRNA half-life is rapidly shortened by IFN-gamma. Dactinomycin 6-19 interferon gamma Homo sapiens 108-117