PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 16233644-2 2004 The transcriptional heterodimeric complex composed of the gene products of INO2 and INO4 binds to a conserved cis-acting upstream activating sequence designated as the inositol-choline responsive element (ICRE), and activates the expression of these genes. Inositol 168-176 Ino4p Saccharomyces cerevisiae S288C 84-88 15145930-6 2004 In contrast, Ino2p and Ino4p are required for full GIT1 expression when inositol is limiting, with or without phosphate limitation, but not when only phosphate is limiting. Inositol 72-80 Ino4p Saccharomyces cerevisiae S288C 23-28 15028711-7 2004 This regulation was mediated through the UAS(INO) element and by the transcription factors Ino2p, Ino4p, and Opi1p that are responsible for the inositol-mediated regulation of UAS(INO)-containing genes involved in phospholipid synthesis. Inositol 144-152 Ino4p Saccharomyces cerevisiae S288C 98-103 12753200-1 2003 In the yeast Saccharomyces cerevisiae, genes involved in phospholipid biosynthesis are activated by ICRE (inositol/choline-responsive element) up-stream motifs and the corresponding heterodimeric binding factor, Ino2 + Ino4. Inositol 106-114 Ino4p Saccharomyces cerevisiae S288C 219-223 12877981-9 2003 In yeast, when inositol is limiting, the heterodimeric transcriptional activator Ino2p/Ino4p derepresses expression of INO1 by binding to the upstream activation sequence UAS(INO). Inositol 15-23 Ino4p Saccharomyces cerevisiae S288C 87-92 10407264-2 1999 The ICRE (inositol/choline-responsive element), which is necessary and sufficient for regulation by phospholipid precursors, functions as a binding site for the heterodimeric Ino2/Ino4 activator. Inositol 10-18 Ino4p Saccharomyces cerevisiae S288C 180-184 11251853-2 2001 Complete derepression of phospholipid biosynthetic gene expression in response to inositol/choline deprivation requires both INO2 and INO4. Inositol 82-90 Ino4p Saccharomyces cerevisiae S288C 134-138 10781542-10 2000 We further report that the INO4 steady-state transcript levels and Ino4p levels are regulated twofold in response to inositol and choline, suggesting a posttranscriptional mechanism of regulation. Inositol 117-125 Ino4p Saccharomyces cerevisiae S288C 27-31 10781542-10 2000 We further report that the INO4 steady-state transcript levels and Ino4p levels are regulated twofold in response to inositol and choline, suggesting a posttranscriptional mechanism of regulation. Inositol 117-125 Ino4p Saccharomyces cerevisiae S288C 67-72 10224245-1 1999 A search was conducted for suppressors of the inositol auxotrophic phenotype of the ino4-8 mutant of yeast. Inositol 46-54 Ino4p Saccharomyces cerevisiae S288C 84-88 10224245-5 1999 The suppressor mutation, sia1-1, identified as an allele of REG1, caused constitutive INO1 expression and was capable of suppressing the inositol auxotrophy of a second ino4 missense mutant, ino4-26, as well as ino2-419, a missense mutation of INO2. Inositol 137-145 Ino4p Saccharomyces cerevisiae S288C 169-173 10224245-5 1999 The suppressor mutation, sia1-1, identified as an allele of REG1, caused constitutive INO1 expression and was capable of suppressing the inositol auxotrophy of a second ino4 missense mutant, ino4-26, as well as ino2-419, a missense mutation of INO2. Inositol 137-145 Ino4p Saccharomyces cerevisiae S288C 191-195 9611229-2 1998 Removal of inositol results in transcriptional activation by heterodimeric complexes of two bHLH proteins, Ino2p and Ino4p. Inositol 11-19 Ino4p Saccharomyces cerevisiae S288C 117-122 10361278-1 1999 Structural genes of phospholipid biosynthesis in the yeast Saccharomyces cerevisiae are activated by the Ino2p/Ino4p transcription factor that binds to ICRE promoter motifs and mediates maximal gene expression in the absence of inositol. Inositol 228-236 Ino4p Saccharomyces cerevisiae S288C 111-116 7862526-0 1995 Yeast transcriptional activator INO2 interacts as an Ino2p/Ino4p basic helix-loop-helix heteromeric complex with the inositol/choline-responsive element necessary for expression of phospholipid biosynthetic genes in Saccharomyces cerevisiae. Inositol 117-125 Ino4p Saccharomyces cerevisiae S288C 59-64 9211788-2 1997 ICRE-dependent gene activation, requiring the regulatory genes INO2 and INO4, is repressed in the presence of the phospholipid precursors inositol and choline. Inositol 138-146 Ino4p Saccharomyces cerevisiae S288C 72-76 9565583-9 1998 In ino2 and ino4 mutants, gene expression was greatly reduced and was not subject to inositol regulation consistent with inositol repression being dependent on the INO2 and INO4 regulatory genes. Inositol 121-129 Ino4p Saccharomyces cerevisiae S288C 173-177 9211788-0 1997 Influence of gene dosage and autoregulation of the regulatory genes INO2 and INO4 on inositol/choline-repressible gene transcription in the yeast Saccharomyces cerevisiae. Inositol 85-93 Ino4p Saccharomyces cerevisiae S288C 77-81 8614637-2 1996 Derepression in response to inositol deprivation requires the INO2 and INO4 regulatory genes. Inositol 28-36 Ino4p Saccharomyces cerevisiae S288C 71-75 7753636-3 1995 INO1-100 suppressed the inositol auxotrophy of ino2, ino4, swi1, swi2 and swi3 mutants. Inositol 24-32 Ino4p Saccharomyces cerevisiae S288C 53-57 7753636-6 1995 A 38 bp deletion from -245 to -208 suppressed the inositol auxotrophy of an ino2 mutant, but not an ino4 mutant, indicating that Ino2p and Ino4p may function alone as well as in a complex. Inositol 50-58 Ino4p Saccharomyces cerevisiae S288C 139-144 7753636-8 1995 A deletion from -167 to -128 suppressed the inositol auxotrophy of swi, ino2 and ino4 mutants, indicating the presence of a previously unidentified URS1. Inositol 44-52 Ino4p Saccharomyces cerevisiae S288C 81-85 7862162-2 1995 In the absence of inositol and choline (derepressing), the products of the INO2 and INO4 genes form a heteromeric complex which binds to a 10-bp element, upstream activation sequence INO (UASINO), in the promoters of the phospholipid biosynthetic genes to activate their transcription. Inositol 18-26 Ino4p Saccharomyces cerevisiae S288C 84-88 1461729-3 1992 We have investigated the influence of a functional INO4 gene previously described as a regulator of inositol biosynthesis on the expression of FAS1 and FAS2. Inositol 100-108 Ino4p Saccharomyces cerevisiae S288C 51-55 8294482-7 1994 The INO2, INO4, and OPI1 genes thus coordinate uptake of inositol to endogenous inositol biosynthesis and to phospholipid biosynthesis. Inositol 57-65 Ino4p Saccharomyces cerevisiae S288C 10-14 8294482-7 1994 The INO2, INO4, and OPI1 genes thus coordinate uptake of inositol to endogenous inositol biosynthesis and to phospholipid biosynthesis. Inositol 80-88 Ino4p Saccharomyces cerevisiae S288C 10-14 20162531-7 2010 The results showed that expression of CIT2-lacZ reporter was induced in a rho(0) strain by the presence of inositol via the Ino2p and Ino4p bHLH proteins, which are known regulators of phospholipid synthesis. Inositol 107-115 Ino4p Saccharomyces cerevisiae S288C 134-138 1650460-4 1991 Formation of the INO2/INO4-dependent complexes was increased when extracts prepared from cells grown under derepressing conditions (i.e. absence of inositol and choline). Inositol 148-156 Ino4p Saccharomyces cerevisiae S288C 22-26 3025587-7 1986 The INO1 transcript was present at a very low level in cells containing mutations (ino2 and ino4) in regulatory genes unlinked to INO1 that result in inositol auxotrophy. Inositol 150-158 Ino4p Saccharomyces cerevisiae S288C 92-96 6392853-9 1984 The pleiotropic phenotype of the ino2 and ino4 mutants described in this paper suggests that the INO2 and INO4 loci are involved in the regulation of phospholipid methylation in the membrane as well as inositol biosynthesis in the cytoplasm. Inositol 202-210 Ino4p Saccharomyces cerevisiae S288C 42-46 6392853-9 1984 The pleiotropic phenotype of the ino2 and ino4 mutants described in this paper suggests that the INO2 and INO4 loci are involved in the regulation of phospholipid methylation in the membrane as well as inositol biosynthesis in the cytoplasm. Inositol 202-210 Ino4p Saccharomyces cerevisiae S288C 106-110 28924045-3 2017 Inositol supplementation, however, favors the translocation of Opi1p from the ER into the nucleus, where it interacts with the Ino2p-Ino4p complex, attenuating transcription of INO1 A strain devoid of Scs2p (scs2Delta) and a mutant, OPI1FFAT, lacking the ability to interact with Scs2p were utilized to examine the specific role(s) of the Opi1p-Scs2p interaction in the regulation of INO1 expression and overall lipid metabolism. Inositol 0-8 Ino4p Saccharomyces cerevisiae S288C 133-138 27743455-2 2017 Gene expression is stimulated by the heterodimeric activator Ino2/Ino4, which binds to ICRE (inositol/choline-responsive element) promoter sequences. Inositol 93-101 Ino4p Saccharomyces cerevisiae S288C 66-70 22673525-1 2012 The regulation of phospholipid biosynthesis in Saccharomyces cerevisiae through cis-acting upstream activating sequence inositol (UAS(ino)) and trans-acting elements, such as the INO2-INO4 complex and OPI1 by inositol supplementation in growth is thoroughly studied. Inositol 209-217 Ino4p Saccharomyces cerevisiae S288C 184-188 2155238-4 1990 An ino4 deletion mutant was constructed by in vitro gene disruption and the deletion mutant was shown to be viable but auxotrophic for inositol. Inositol 135-143 Ino4p Saccharomyces cerevisiae S288C 3-7 2834106-1 1988 Yeast ino4 mutants are auxotrophic for the phospholipid precursor inositol and have pleiotropic defects in phospholipid synthesis. Inositol 66-74 Ino4p Saccharomyces cerevisiae S288C 6-10 6392853-4 1984 The ino2 and ino4 mutants, originally isolated on the basis of an inositol requirement, are unable to derepress the cytoplasmic enzyme inositol-1-phosphate synthase (myo-inositol-1-phosphate synthase; EC 5.5.1.4). Inositol 66-74 Ino4p Saccharomyces cerevisiae S288C 13-17 17249096-0 1981 Inositol Mutants of SACCHAROMYCES CEREVISIAE: Mapping the ino1 Locus and Characterizing Alleles of the ino1, ino2 and ino4 Loci. Inositol 0-8 Ino4p Saccharomyces cerevisiae S288C 118-122 28827025-6 2018 The regulation of the CHO1 expression is mediated through the inositol-sensitive upstream activation sequence (UASINO), a cis-acting element for the phosphatidate-controlled Henry (Ino2-Ino4/Opi1) regulatory circuit. Inositol 62-70 Ino4p Saccharomyces cerevisiae S288C 186-190 22182244-5 2012 This study tested the ability of all yeast bHLH proteins to regulate PHO5 expression and identified inositol-mediated regulation via the Ino2p/Ino4p bHLH proteins. Inositol 100-108 Ino4p Saccharomyces cerevisiae S288C 143-147 18542964-8 2008 We could show that Ino2-dependent activation of a lexA-Ino4 fusion is affected by inositol and choline. Inositol 82-90 Ino4p Saccharomyces cerevisiae S288C 55-59 18542964-1 2008 Structural genes of phospholipid biosynthesis in the yeast S. cerevisiae are activated by the heterodimeric transcription factor Ino2 + Ino4, binding to ICRE (inositol/choline-responsive element) promoter motifs. Inositol 159-167 Ino4p Saccharomyces cerevisiae S288C 136-140 17090927-1 2006 Yeast Ino2p-Ino4p heterodimeric complex is well known as a transcriptional activator for the genes regulated by inositol and choline, such as the INO1 gene. Inositol 112-120 Ino4p Saccharomyces cerevisiae S288C 12-17 17588177-1 2007 In the yeast Saccharomyces cerevisiae, structural genes of phospholipid biosynthesis are activated by a heterodimer of basic helix-loop-helix proteins, Ino2 and Ino4, which bind to the inositol/choline-responsive element (ICRE) UAS element. Inositol 185-193 Ino4p Saccharomyces cerevisiae S288C 161-165 15755922-3 2005 Most of the phospholipid biosynthetic genes are regulated in response to inositol and choline via a regulatory circuit that includes the Ino2p:Ino4p activator complex and the Opi1p repressor. Inositol 73-81 Ino4p Saccharomyces cerevisiae S288C 143-148 15611057-0 2005 Genome-wide analysis reveals inositol, not choline, as the major effector of Ino2p-Ino4p and unfolded protein response target gene expression in yeast. Inositol 29-37 Ino4p Saccharomyces cerevisiae S288C 83-88 16544270-10 2006 However, expression was elevated in an ino4delta mutant but not in ino2delta cells, suggesting multiple and separate functions for the inositol-responsive INO2/INO4 gene products, which normally function as a dimer in regulating gene function. Inositol 135-143 Ino4p Saccharomyces cerevisiae S288C 160-164 15819625-1 2005 Regulated expression of structural genes involved in yeast phospholipid biosynthesis is mediated by inositol/choline-responsive element (ICRE) upstream motifs, bound by the heterodimeric activator complex Ino2 + Ino4. Inositol 100-108 Ino4p Saccharomyces cerevisiae S288C 212-216