PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 17376394-2 2007 The first committed step in the synthesis of leukotrienes is the conversion of arachidonic acid to leukotriene A(4) (LTA(4)) in two successive reactions catalyzed by 5-lipoxygenase (5-LOX). Leukotriene A4 99-112 arachidonate 5-lipoxygenase Homo sapiens 166-180 27033421-1 2016 Human neutrophil 5-lipoxygenase (5-LOX) oxidizes arachidonic acid (AA) to 5S-hydro(pero)xy-6E,8Z,11Z, 14Z-eicosatetraenoic acid (5-H(p)ETE) and leukotriene (LT)A4, which is further converted to the chemoattractant LTB4. Leukotriene A4 157-162 arachidonate 5-lipoxygenase Homo sapiens 17-31 27477678-2 2016 They are formed by 5-lipoxygenase (5-LOX) from arachidonic acid (AA) yielding the unstable leukotriene A4 (LTA4) that is subsequently conjugated with glutathione (GSH) by LTC4 synthase (LTC4S). Leukotriene A4 91-105 arachidonate 5-lipoxygenase Homo sapiens 19-33 24893149-1 2014 5-Lipoxygenase (5-LOX) reacts with arachidonic acid (AA) to first generate 5(S)-hydroperoxy-6(E),8(Z),11(Z),14(Z)-eicosatetraenoic acid [5(S)-HpETE] and then an epoxide from 5(S)-HpETE to form leukotriene A4, from a single polyunsaturated fatty acid. Leukotriene A4 193-207 arachidonate 5-lipoxygenase Homo sapiens 0-14 24893149-1 2014 5-Lipoxygenase (5-LOX) reacts with arachidonic acid (AA) to first generate 5(S)-hydroperoxy-6(E),8(Z),11(Z),14(Z)-eicosatetraenoic acid [5(S)-HpETE] and then an epoxide from 5(S)-HpETE to form leukotriene A4, from a single polyunsaturated fatty acid. Leukotriene A4 193-207 arachidonate 5-lipoxygenase Homo sapiens 16-21 17376394-2 2007 The first committed step in the synthesis of leukotrienes is the conversion of arachidonic acid to leukotriene A(4) (LTA(4)) in two successive reactions catalyzed by 5-lipoxygenase (5-LOX). Leukotriene A4 99-112 arachidonate 5-lipoxygenase Homo sapiens 182-187 10864449-1 2000 Leukotriene A(4) (LTA(4)) is a chemically reactive conjugated triene epoxide that is formed by 5-lipoxygenase and is an intermediate in the formation of the biologically active eicosanoids leukotriene B(4) and leukotriene C(4). Leukotriene A4 0-13 arachidonate 5-lipoxygenase Homo sapiens 95-109 11802780-1 2002 5-Lipoxygenase (5-LO) metabolizes arachidonic acid to leukotriene A4, a key intermediate in leukotriene biosynthesis. Leukotriene A4 54-68 arachidonate 5-lipoxygenase Homo sapiens 0-14 11802780-1 2002 5-Lipoxygenase (5-LO) metabolizes arachidonic acid to leukotriene A4, a key intermediate in leukotriene biosynthesis. Leukotriene A4 54-68 arachidonate 5-lipoxygenase Homo sapiens 16-20 11087405-4 2000 The wild-type 5-lipoxygenase converts 5S-HpETE almost exclusively to leukotriene A(4) as indicated by the dominant formation of leukotriene A(4) hydrolysis products. Leukotriene A4 69-82 arachidonate 5-lipoxygenase Homo sapiens 14-28 11087405-4 2000 The wild-type 5-lipoxygenase converts 5S-HpETE almost exclusively to leukotriene A(4) as indicated by the dominant formation of leukotriene A(4) hydrolysis products. Leukotriene A4 128-141 arachidonate 5-lipoxygenase Homo sapiens 14-28 15342681-1 2004 Leukotriene A(4) (LTA(4)) is a chemically reactive conjugated triene epoxide product derived from 5-lipoxygenase oxygenation of arachidonic acid. Leukotriene A4 0-13 arachidonate 5-lipoxygenase Homo sapiens 98-112 15342681-1 2004 Leukotriene A(4) (LTA(4)) is a chemically reactive conjugated triene epoxide product derived from 5-lipoxygenase oxygenation of arachidonic acid. Leukotriene A4 18-21 arachidonate 5-lipoxygenase Homo sapiens 98-112 11796736-1 2002 The activation of 5-lipoxygenase (5-LO) involves its calcium-dependent translocation to the nuclear envelope, where it catalyzes the two-step transformation of arachidonic acid into leukotriene A(4), leading to the synthesis of various leukotrienes. Leukotriene A4 182-195 arachidonate 5-lipoxygenase Homo sapiens 18-32 11796736-1 2002 The activation of 5-lipoxygenase (5-LO) involves its calcium-dependent translocation to the nuclear envelope, where it catalyzes the two-step transformation of arachidonic acid into leukotriene A(4), leading to the synthesis of various leukotrienes. Leukotriene A4 182-195 arachidonate 5-lipoxygenase Homo sapiens 34-38 10864449-1 2000 Leukotriene A(4) (LTA(4)) is a chemically reactive conjugated triene epoxide that is formed by 5-lipoxygenase and is an intermediate in the formation of the biologically active eicosanoids leukotriene B(4) and leukotriene C(4). Leukotriene A4 18-21 arachidonate 5-lipoxygenase Homo sapiens 95-109 10591081-1 1999 5-Lipoxygenase (5-LO) catalyzes the two-step conversion of arachidonic acid to leukotriene A4 (LTA4). Leukotriene A4 79-93 arachidonate 5-lipoxygenase Homo sapiens 0-14 7938075-1 1994 5-Lipoxygenase catalyzes the transformation of arachidonic acid to leukotriene A4. Leukotriene A4 67-81 arachidonate 5-lipoxygenase Homo sapiens 0-14 9620944-1 1998 Cytosolic phospholipase A2 translocates from the cytoplasm to selectively hydrolyze nuclear envelope phospholipids, releasing free arachidonate. 5-Lipoxygenase-activating protein, an arachidonate transfer protein, then binds arachidonate and presents it to 5-lipoxygenase (5-LO), which catalyzes a two-step reaction to produce leukotriene A4. In resting human and rat peripheral blood neutrophils, 5-LO is localized to the cytoplasm; in rat basophilic leukemia cells and human alveolar macrophages, however, it is found predominantly in the nucleus. Leukotriene A4 328-342 arachidonate 5-lipoxygenase Homo sapiens 146-160 7840802-2 1995 The present study shows for the first time that endogenous arachidonic acid metabolism via 5-lipoxygenase pathway is significantly increased after glucocorticoid administration, leading to increased generation of the unstable precursor leukotriene A4 (LTA4) followed by predominant non-enzymatic LTA4 opening and leukotriene B4 (LTB4) omega-hydroxylation pathway. Leukotriene A4 236-250 arachidonate 5-lipoxygenase Homo sapiens 91-105 7929400-1 1994 5-Lipoxygenase is the first committed enzyme in the leukotriene biosynthetic pathway and is known to catalyze not only the first oxygenation of arachidonate to form 5(S)-hydroperoxyeicosatetraenoic acid (5(S)-HPETE), but also dehydration of this intermediate into leukotriene A4 (LTA4) by an activity termed leukotriene A4 synthase. Leukotriene A4 264-278 arachidonate 5-lipoxygenase Homo sapiens 0-14 7929400-1 1994 5-Lipoxygenase is the first committed enzyme in the leukotriene biosynthetic pathway and is known to catalyze not only the first oxygenation of arachidonate to form 5(S)-hydroperoxyeicosatetraenoic acid (5(S)-HPETE), but also dehydration of this intermediate into leukotriene A4 (LTA4) by an activity termed leukotriene A4 synthase. Leukotriene A4 264-278 arachidonate 5-lipoxygenase Homo sapiens 308-331 9609696-1 1998 5-Lipoxygenase (5-LO) catalyzes the formation of 5-hydroperoxy-eicosatetraenoic acid (5-HPETE) and leukotriene A4 (LTA4) from arachidonic acid. Leukotriene A4 99-113 arachidonate 5-lipoxygenase Homo sapiens 0-14 9647604-3 1998 5-Lipoxygenase-activating protein, an arachidonate transfer protein, then binds arachidonate and presents it to 5-lipoxygenase (5-LO), which catalyzes a two-step reaction to produce leukotriene A4. Leukotriene A4 182-196 arachidonate 5-lipoxygenase Homo sapiens 0-14 9647604-3 1998 5-Lipoxygenase-activating protein, an arachidonate transfer protein, then binds arachidonate and presents it to 5-lipoxygenase (5-LO), which catalyzes a two-step reaction to produce leukotriene A4. Leukotriene A4 182-196 arachidonate 5-lipoxygenase Homo sapiens 112-126 8215451-1 1993 Leukotriene A4 (LTA4), the reaction product of 5-lipoxygenase in human polymorphonuclear (PMN) leukocytes, is transformed both to LTB4 and a mixture of 5,6- and 5,12-dihydroxy-eicosatetraenoic acids (diHETE) via nonenzymatic hydrolysis. Leukotriene A4 16-20 arachidonate 5-lipoxygenase Homo sapiens 47-61 8300592-0 1994 Irreversible inactivation of 5-lipoxygenase by leukotriene A4. Leukotriene A4 47-61 arachidonate 5-lipoxygenase Homo sapiens 29-43 8300592-2 1994 We report that leukotriene A4, the electrophilic product of 5-lipoxygenase catalysis, irreversibly inactivates the enzyme. Leukotriene A4 15-29 arachidonate 5-lipoxygenase Homo sapiens 60-74 8300592-5 1994 Leukotriene A4 inactivates cellular 5-lipoxygenase without inhibiting its translocation from the cytosol to the membrane, suggesting that it impairs catalysis without impairing formation of the complex between 5-lipoxygenase and its membrane-associated activating protein. Leukotriene A4 0-14 arachidonate 5-lipoxygenase Homo sapiens 36-50 8300592-5 1994 Leukotriene A4 inactivates cellular 5-lipoxygenase without inhibiting its translocation from the cytosol to the membrane, suggesting that it impairs catalysis without impairing formation of the complex between 5-lipoxygenase and its membrane-associated activating protein. Leukotriene A4 0-14 arachidonate 5-lipoxygenase Homo sapiens 210-224 8300592-6 1994 Consistent with this, leukotriene A4 inactivates purified 5-lipoxygenase from human neutrophils, via saturable, pseudo first-order kinetics with a rate constant, ki = 0.14 min-1 and a dissociation constant, Ki = 2.1 +/- 0.7 microM. Leukotriene A4 22-36 arachidonate 5-lipoxygenase Homo sapiens 58-72 8300592-8 1994 Preincubation with leukotriene A4 diminished the incorporation of radiolabeled material, consistent with irreversible modification of 5-lipoxygenase by its metastable product, leukotriene A4. Leukotriene A4 19-33 arachidonate 5-lipoxygenase Homo sapiens 134-148 8300592-8 1994 Preincubation with leukotriene A4 diminished the incorporation of radiolabeled material, consistent with irreversible modification of 5-lipoxygenase by its metastable product, leukotriene A4. Leukotriene A4 176-190 arachidonate 5-lipoxygenase Homo sapiens 134-148 8269937-4 1993 When Sf9 cells expressing 5-lipoxygenase are incubated in the presence of A23187 and arachidonic acid, (5S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid 5-H(P)ETE and LTA4 are synthesized in a ratio of 5:1 for 5-H(P)ETE/LT. Leukotriene A4 176-180 arachidonate 5-lipoxygenase Homo sapiens 26-40 8215451-1 1993 Leukotriene A4 (LTA4), the reaction product of 5-lipoxygenase in human polymorphonuclear (PMN) leukocytes, is transformed both to LTB4 and a mixture of 5,6- and 5,12-dihydroxy-eicosatetraenoic acids (diHETE) via nonenzymatic hydrolysis. Leukotriene A4 0-14 arachidonate 5-lipoxygenase Homo sapiens 47-61 8395245-0 1993 Interfacial catalysis and production of a high ratio of leukotriene A4 to 5-HPETE by 5-lipoxygenase in a coupled assay with phospholipase A2. Leukotriene A4 56-70 arachidonate 5-lipoxygenase Homo sapiens 85-99 2300172-2 1990 The initial enzymatic step in the formation of leukotrienes is the oxidation of arachidonic acid by 5-lipoxygenase (5-LO) to leukotriene A4. Leukotriene A4 125-139 arachidonate 5-lipoxygenase Homo sapiens 100-114 1848236-4 1991 The reaction products of arachidonic acid oxidation were 5-HPETE and 6-trans- and 12-epi-6-trans-leukotriene B4, the nonenzymatic hydrolysis products of leukotriene A4 (LTA4), indicating that the purified protein expressed both the 5-oxygenase and leukotriene A4 synthase activities (ratio 6:1). Leukotriene A4 153-167 arachidonate 5-lipoxygenase Homo sapiens 248-271 1429555-5 1992 The membrane-associated 5-lipoxygenase was two times more efficient in the production of leukotriene A4 from arachidonate-derived 5-hydroperoxyeicosatetraenoic acid than the cytosolic enzyme. Leukotriene A4 89-103 arachidonate 5-lipoxygenase Homo sapiens 24-38 3036460-5 1987 Formation of the leukotrienes from arachidonic acid is initiated by the action of 5-lipoxygenase producing leukotriene A4. Leukotriene A4 107-121 arachidonate 5-lipoxygenase Homo sapiens 82-96 2649885-4 1989 The recombinant 5-lipoxygenase in 10,000 x g supernatant fractions was able to synthesize large amounts of 5-hydroperoxy-6,8,10,14-icosatetraenoic acid, together with smaller amounts of the nonenzymatic hydrolysis products of leukotriene A4, and exhibited a dependence on Ca2+ and ATP for maximal activity. Leukotriene A4 226-240 arachidonate 5-lipoxygenase Homo sapiens 16-30 6811315-0 1982 Selective inhibition of 5-lipoxygenase by 5,6-methanoleukotriene A4, a stable analogue of leukotriene A4. Leukotriene A4 53-67 arachidonate 5-lipoxygenase Homo sapiens 24-38 3020619-0 1986 Stereoselective hydrogen abstraction in leukotriene A4 synthesis by purified 5-lipoxygenase of porcine leukocytes. Leukotriene A4 40-54 arachidonate 5-lipoxygenase Homo sapiens 77-91 3006030-6 1986 When incubated with arachidonic acid, the purified 5-lipoxygenase converted approximately equal to 15% of its endogenously generated 5-hydroperoxyicosatetraenoic acid (5-HPETE) to LTA4. Leukotriene A4 180-184 arachidonate 5-lipoxygenase Homo sapiens 51-65