PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 32344570-8 2020 Acamprosate inhibited OAT1-mediated p-aminohippuric acid (PAH) uptake but did not inhibit substrate uptake via OAT3 expressing cells, neither when applied concomitantly nor after a 3 h preincubation with acamprosate. p-Aminohippuric Acid 36-56 solute carrier family 22 member 6 Homo sapiens 22-26 32981893-5 2020 In structure-activity relationship analyses, removal of either the 3-halogen or 4-hydroxyl group from FAMT or its structural analog 3-iodo-alpha-methyl-l-tyrosine greatly decreased the interaction with OAT1, reducing the [14C]p-aminohippurate uptake inhibition and the efflux induction. p-Aminohippuric Acid 226-242 solute carrier family 22 member 6 Homo sapiens 202-206 32234809-6 2020 We showed that bortezomib and carfilzomib extremely increased the ubiquitinated OAT1, which correlated well with an enhanced OAT1-mediated transport of p-aminohippuric acid and an enhanced OAT1 surface expression. p-Aminohippuric Acid 152-172 solute carrier family 22 member 6 Homo sapiens 80-84 32234809-6 2020 We showed that bortezomib and carfilzomib extremely increased the ubiquitinated OAT1, which correlated well with an enhanced OAT1-mediated transport of p-aminohippuric acid and an enhanced OAT1 surface expression. p-Aminohippuric Acid 152-172 solute carrier family 22 member 6 Homo sapiens 125-129 32234809-6 2020 We showed that bortezomib and carfilzomib extremely increased the ubiquitinated OAT1, which correlated well with an enhanced OAT1-mediated transport of p-aminohippuric acid and an enhanced OAT1 surface expression. p-Aminohippuric Acid 152-172 solute carrier family 22 member 6 Homo sapiens 125-129 32344570-8 2020 Acamprosate inhibited OAT1-mediated p-aminohippuric acid (PAH) uptake but did not inhibit substrate uptake via OAT3 expressing cells, neither when applied concomitantly nor after a 3 h preincubation with acamprosate. p-Aminohippuric Acid 58-61 solute carrier family 22 member 6 Homo sapiens 22-26 32344570-9 2020 The uptake of PAH via OAT1 was inhibited in a competitive manner by acamprosate and cellular uptake studies showed that acamprosate is a substrate for OAT1 with a Km-value of approximately 700 microM. p-Aminohippuric Acid 14-17 solute carrier family 22 member 6 Homo sapiens 22-26 32344570-9 2020 The uptake of PAH via OAT1 was inhibited in a competitive manner by acamprosate and cellular uptake studies showed that acamprosate is a substrate for OAT1 with a Km-value of approximately 700 microM. p-Aminohippuric Acid 14-17 solute carrier family 22 member 6 Homo sapiens 151-155 27614971-3 2017 The prototypical substrate for renal organic anion transport systems, para-aminohippurate (PAH), is transported across basolateral membranes of proximal tubular cells via OAT1 (SLC22A6) and OAT3 (SLC22A8) against an electrochemical gradient in exchange for intracellular dicarboxylates. p-Aminohippuric Acid 70-89 solute carrier family 22 member 6 Homo sapiens 171-175 29244191-13 2018 Finally, the OAT1-substrate para-aminohippurate (PAH) showed some uptake (Km -value of 2.0 +- 0.4 mM) but did not interact with cyclic nucleotide or indomethacin transport. p-Aminohippuric Acid 28-47 solute carrier family 22 member 6 Homo sapiens 13-17 29244191-13 2018 Finally, the OAT1-substrate para-aminohippurate (PAH) showed some uptake (Km -value of 2.0 +- 0.4 mM) but did not interact with cyclic nucleotide or indomethacin transport. p-Aminohippuric Acid 49-52 solute carrier family 22 member 6 Homo sapiens 13-17 32373203-4 2020 Probenecid, p-aminohippurate, and benzylpenicillin inhibited the uptake of imipenem and cilastatin in rat kidney slices and in hOAT1- and hOAT3-HEK 293 cells, respectively. p-Aminohippuric Acid 12-28 solute carrier family 22 member 6 Homo sapiens 127-132 27614971-3 2017 The prototypical substrate for renal organic anion transport systems, para-aminohippurate (PAH), is transported across basolateral membranes of proximal tubular cells via OAT1 (SLC22A6) and OAT3 (SLC22A8) against an electrochemical gradient in exchange for intracellular dicarboxylates. p-Aminohippuric Acid 70-89 solute carrier family 22 member 6 Homo sapiens 177-184 27614971-3 2017 The prototypical substrate for renal organic anion transport systems, para-aminohippurate (PAH), is transported across basolateral membranes of proximal tubular cells via OAT1 (SLC22A6) and OAT3 (SLC22A8) against an electrochemical gradient in exchange for intracellular dicarboxylates. p-Aminohippuric Acid 91-94 solute carrier family 22 member 6 Homo sapiens 171-175 27614971-3 2017 The prototypical substrate for renal organic anion transport systems, para-aminohippurate (PAH), is transported across basolateral membranes of proximal tubular cells via OAT1 (SLC22A6) and OAT3 (SLC22A8) against an electrochemical gradient in exchange for intracellular dicarboxylates. p-Aminohippuric Acid 91-94 solute carrier family 22 member 6 Homo sapiens 177-184 25477469-4 2015 We examined the effects of structural analogs of p-aminohippurate (PAH), melphalan, chlorambucil, and bendamustine, on OAT1-mediated [(3)H]PAH uptake and OAT3- and OAT4-mediated [(3)H]estrone sulfate (ES) uptake in stably transfected human embryonic kidney-293 cells. p-Aminohippuric Acid 49-65 solute carrier family 22 member 6 Homo sapiens 119-123 25477469-5 2015 Melphalan had no significant inhibitory effect on any OAT, whereas chlorambucil reduced OAT1-, OAT3-, and OAT4-mediated uptake of PAH or ES down to 14.6%, 16.3%, and 66.0% of control, respectively. p-Aminohippuric Acid 130-133 solute carrier family 22 member 6 Homo sapiens 88-92 25477469-4 2015 We examined the effects of structural analogs of p-aminohippurate (PAH), melphalan, chlorambucil, and bendamustine, on OAT1-mediated [(3)H]PAH uptake and OAT3- and OAT4-mediated [(3)H]estrone sulfate (ES) uptake in stably transfected human embryonic kidney-293 cells. p-Aminohippuric Acid 67-70 solute carrier family 22 member 6 Homo sapiens 119-123 25477469-4 2015 We examined the effects of structural analogs of p-aminohippurate (PAH), melphalan, chlorambucil, and bendamustine, on OAT1-mediated [(3)H]PAH uptake and OAT3- and OAT4-mediated [(3)H]estrone sulfate (ES) uptake in stably transfected human embryonic kidney-293 cells. p-Aminohippuric Acid 139-142 solute carrier family 22 member 6 Homo sapiens 119-123 26640952-6 2015 METHOD: To demonstrate interaction of ICA with human OAT1, OAT2, OAT3 and OAT4, ICA was tested on these transporters stably transfected in HEK293 cells by using p-aminohippurate (PAH), cGMP and estrone-3-sulfate (ES) as reference substrates, respectively. p-Aminohippuric Acid 179-182 solute carrier family 22 member 6 Homo sapiens 53-57 26640952-7 2015 RESULTS: Uptakes of PAH and ES in OAT1- and OAT3-transfected HEK293 cells were inhibited by ICA with half-maximal inhibition values of 0.29 +- 0.05 and 2.58 +- 0.16 microM, respectively. p-Aminohippuric Acid 20-23 solute carrier family 22 member 6 Homo sapiens 34-38 24185403-8 2014 The uptake of p-aminohippurate by hOAT1 and estron-3-sulfate by hOAT3 was decreased with increasing PCS concentration. p-Aminohippuric Acid 14-30 solute carrier family 22 member 6 Homo sapiens 34-39 25239859-7 2014 Furthermore, omeprazole, lansoprazole, and pantoprazole exhibited inhibited PAH uptake on hOAT1 in a concentration-dependent manner (IC50 = 4.32 +- 1.26, 7.58 +- 1.06, and 63.21 +- 4.74 microM, respectively). p-Aminohippuric Acid 76-79 solute carrier family 22 member 6 Homo sapiens 90-95 22728397-4 2012 JBP485 (a substrate for OAT1 and OAT3), p-aminohippurate (PAH) (a substrate for OAT1) and benzylpenicillin (PCG) (a substrate for OAT3) could decrease the uptake of acyclovir in kidney slices and in hOAT1-/hOAT3-HEK293 cells. p-Aminohippuric Acid 40-56 solute carrier family 22 member 6 Homo sapiens 80-84 24747579-9 2014 These results suggest that adefovir and benzylpenicillin can be used as probe drugs for OAT1 and OAT3, respectively, and that PAH can be used to investigate the role of OAT1 in the urinary excretion of drugs in humans, whereas it may modulate other transport processes in the kidney. p-Aminohippuric Acid 126-129 solute carrier family 22 member 6 Homo sapiens 169-173 23255614-5 2013 In OAT1-transfected cells, GSH reduced the uptake of PAH marginally. p-Aminohippuric Acid 53-56 solute carrier family 22 member 6 Homo sapiens 3-7 23255614-8 2013 The GSH precursor dipeptide, cysteinyl glycine (cysgly), and the glutamate derivative N-acetyl glutamate (NAG), inhibited uptake of PAH when present in the medium and trans-stimulated uptake of PAH from the intracellular side, indicating that they are hitherto unrecognized transported substrates of OAT1. p-Aminohippuric Acid 132-135 solute carrier family 22 member 6 Homo sapiens 300-304 22875277-7 2012 NOG, 2,4-DPD and PDCA, but not DMOG, inhibited PAH uptake by OAT1 significantly. p-Aminohippuric Acid 47-50 solute carrier family 22 member 6 Homo sapiens 61-65 25002746-5 2014 Most flavonoid aglycones produced substantial decreases in PAH uptake in OAT1-expressing cells. p-Aminohippuric Acid 59-62 solute carrier family 22 member 6 Homo sapiens 73-77 25002746-6 2014 Among the flavonoids screened, fisetin, luteolin, morin, and quercetin exhibited the strongest effect and produced complete inhibition of OAT1-mediated PAH uptake at a concentration of 50 muM. p-Aminohippuric Acid 152-155 solute carrier family 22 member 6 Homo sapiens 138-142 23313623-8 2013 In hOAT1- and hOAT3-HEK293 cells, uptake of entecavir was significantly higher compared to vector-HEK293 cells and was markedly inhibited by p-aminohippurate, benzylpenicillin, and JBP485. p-Aminohippuric Acid 141-157 solute carrier family 22 member 6 Homo sapiens 3-8 22728397-4 2012 JBP485 (a substrate for OAT1 and OAT3), p-aminohippurate (PAH) (a substrate for OAT1) and benzylpenicillin (PCG) (a substrate for OAT3) could decrease the uptake of acyclovir in kidney slices and in hOAT1-/hOAT3-HEK293 cells. p-Aminohippuric Acid 40-56 solute carrier family 22 member 6 Homo sapiens 199-204 22728397-4 2012 JBP485 (a substrate for OAT1 and OAT3), p-aminohippurate (PAH) (a substrate for OAT1) and benzylpenicillin (PCG) (a substrate for OAT3) could decrease the uptake of acyclovir in kidney slices and in hOAT1-/hOAT3-HEK293 cells. p-Aminohippuric Acid 58-61 solute carrier family 22 member 6 Homo sapiens 80-84 22728397-4 2012 JBP485 (a substrate for OAT1 and OAT3), p-aminohippurate (PAH) (a substrate for OAT1) and benzylpenicillin (PCG) (a substrate for OAT3) could decrease the uptake of acyclovir in kidney slices and in hOAT1-/hOAT3-HEK293 cells. p-Aminohippuric Acid 58-61 solute carrier family 22 member 6 Homo sapiens 199-204 22169006-3 2012 Exposure of renal S2 cells expressing hOAT1 to LXR agonists (TO901317 and GW3965) and their endogenous ligand [22(R)-hydroxycholesterol] led to the inhibition of hOAT1-mediated [(14)C]PAH uptake. p-Aminohippuric Acid 184-187 solute carrier family 22 member 6 Homo sapiens 38-43 21697612-3 2011 Injection of hOAT1 and hOAT3 cRNA into oocytes stimulated uptake of typical substrates of hOAT1 and hOAT3 (p-aminohippurate and estrone sulfate, respectively); among the three compounds tested, caffeic acid most strongly inhibited these transporters. p-Aminohippuric Acid 107-123 solute carrier family 22 member 6 Homo sapiens 13-18 22108572-3 2012 The uptake of p-aminohippurate in hOAT1-expressing oocytes and of estrone sulfate in hOAT3-expressing oocytes was strongly inhibited by kynurenic acid, and other tryptophan catabolites, kynurenine and quinolinic acid, showed moderate and no inhibition, respectively. p-Aminohippuric Acid 14-30 solute carrier family 22 member 6 Homo sapiens 34-39 21561794-4 2011 Results demonstrated that diazepam, triazolam, amitriptyline, mianserin, malathion, fenitrothion, chlorpyrifosmethyl, and probenecid significantly inhibited representative substrates of hOAT1 and para-aminohippuric acid uptake by hOAT1. p-Aminohippuric Acid 196-219 solute carrier family 22 member 6 Homo sapiens 186-191 21561794-4 2011 Results demonstrated that diazepam, triazolam, amitriptyline, mianserin, malathion, fenitrothion, chlorpyrifosmethyl, and probenecid significantly inhibited representative substrates of hOAT1 and para-aminohippuric acid uptake by hOAT1. p-Aminohippuric Acid 196-219 solute carrier family 22 member 6 Homo sapiens 230-235 22273603-5 2012 p-Aminohippuric acid (PAH), a substrate of organic anion transporter (OAT) 1, benzylpenicillin (PCG), a substrate of OAT3 and JBP485, a substrate of OAT1 and OAT3, reduced the uptake of bestatin in rat kidney slices and in hOAT1- or hOAT3-HEK 293 cells. p-Aminohippuric Acid 0-20 solute carrier family 22 member 6 Homo sapiens 223-228 21244849-9 2011 Quercetin-3"-O-sulfate strongly inhibited OAT1-mediated p-aminohippuric acid uptake with an IC(50) of 1.22muM. p-Aminohippuric Acid 56-76 solute carrier family 22 member 6 Homo sapiens 42-46 21697612-3 2011 Injection of hOAT1 and hOAT3 cRNA into oocytes stimulated uptake of typical substrates of hOAT1 and hOAT3 (p-aminohippurate and estrone sulfate, respectively); among the three compounds tested, caffeic acid most strongly inhibited these transporters. p-Aminohippuric Acid 107-123 solute carrier family 22 member 6 Homo sapiens 90-95 19394312-7 2009 SMF was a competitive inhibitor of p-aminohippurate uptake by hOAT1 and estrone sulfate uptake by hOAT3 with K(i) values of 225 microM and 1.5mM, respectively. p-Aminohippuric Acid 35-51 solute carrier family 22 member 6 Homo sapiens 62-67 19643159-5 2009 AA potently inhibited the uptake of characteristic substrates, p-aminohippurate for hOAT1 and estrone sulfate for hOAT3 and hOAT4. p-Aminohippuric Acid 63-79 solute carrier family 22 member 6 Homo sapiens 84-89 20460822-3 2010 The apparent 50% inhibitory concentration (IC(50)) of aminopterin for p-aminohippurate uptake by hOAT1 was lower than that of methotrexate (methotrexate: 998 microM, aminopterin: 160 microM). p-Aminohippuric Acid 70-86 solute carrier family 22 member 6 Homo sapiens 97-102 20877132-4 2010 The apparent 50% inhibitory concentrations of lumiracoxib were estimated to be 3.3 microM and 1.9 microM for uptake of p-aminohippurate by hOAT1 and of estrone sulfate by hOAT3, respectively. p-Aminohippuric Acid 119-135 solute carrier family 22 member 6 Homo sapiens 139-144 19439489-6 2009 The renal secretion of YM-385461, one derivative of p-aminohippuric acid, via hOAT1/rOat1, and hepatic uptake of YM-252124 via hOCT1/rOct1 was also expected. p-Aminohippuric Acid 52-72 solute carrier family 22 member 6 Homo sapiens 78-83 17639024-7 2007 MPMA was a competitive inhibitor of p-aminohippurate uptake by OAT1 and estrone sulfate uptake by OAT3 with K(i) values of 14.5 microM and 1.5 microM, respectively. p-Aminohippuric Acid 36-52 solute carrier family 22 member 6 Homo sapiens 63-67 18930752-7 2008 PAH secretion across the monolayer consisted of the uptake of PAH across the basolateral membrane by OAT1 and OAT3 and the apical exit of PAH by a probenecid and MK571-sensitive route consistent with actions of MRP2 or MRP4. p-Aminohippuric Acid 0-3 solute carrier family 22 member 6 Homo sapiens 101-105 18930752-7 2008 PAH secretion across the monolayer consisted of the uptake of PAH across the basolateral membrane by OAT1 and OAT3 and the apical exit of PAH by a probenecid and MK571-sensitive route consistent with actions of MRP2 or MRP4. p-Aminohippuric Acid 62-65 solute carrier family 22 member 6 Homo sapiens 101-105 18930752-7 2008 PAH secretion across the monolayer consisted of the uptake of PAH across the basolateral membrane by OAT1 and OAT3 and the apical exit of PAH by a probenecid and MK571-sensitive route consistent with actions of MRP2 or MRP4. p-Aminohippuric Acid 62-65 solute carrier family 22 member 6 Homo sapiens 101-105 17920288-3 2007 Among tested flavonoids, morin and silybin exhibited significant inhibition effects on the cellular uptake of [3H]-para-aminohippuric acid ([3H]-PAH) in MDCK-hOAT1 cells with Ki of 0.46 microM and 24 microM, respectively, while all the tested flavonoids appeared to be less interactive with hOAT3 compared to hOAT1. p-Aminohippuric Acid 145-148 solute carrier family 22 member 6 Homo sapiens 158-163 17920288-4 2007 A kinetic study suggested that morin and silybin inhibited hOAT1-mediated cellular uptake of [3H]-PAH in a competitive manner. p-Aminohippuric Acid 98-101 solute carrier family 22 member 6 Homo sapiens 59-64 17312013-10 2007 We conclude that reperfused, transplanted kidneys exhibit maldistribution of hOAT1 in proximal tubule cells, resulting in impairment of PAH clearance. p-Aminohippuric Acid 136-139 solute carrier family 22 member 6 Homo sapiens 77-82 17353191-8 2007 PAH uptake by wild type hOAT1 was stimulated in the presence of chloride, whereas the R466K mutant was chloride-insensitive. p-Aminohippuric Acid 0-3 solute carrier family 22 member 6 Homo sapiens 24-29 17255469-9 2007 Taken together, it is likely that the uptake of PAH and 2,4-D is due to OAT1, and the uptake of PCG and ES and part of DHEAS uptake are due to OAT3 in human kidney slices. p-Aminohippuric Acid 48-51 solute carrier family 22 member 6 Homo sapiens 72-76 16038872-4 2005 Inhibitory effects of xanthine- and uric acid-related compounds on the transport of p-aminohippuric acid were examined using CHO-K1 cells stably expressing hOAT1. p-Aminohippuric Acid 84-104 solute carrier family 22 member 6 Homo sapiens 156-161 17498396-8 2007 RESULTS: MPAG inhibited the uptake of PAH via hOAT1 and hOAT3, and calculated IC50 values were 222.6+/-26.6 microM and 41.5+/-11.5 microM, respectively. p-Aminohippuric Acid 38-41 solute carrier family 22 member 6 Homo sapiens 46-51 16597690-5 2006 Stable isomers of 1-SMP, (2-SMP and 4-SMP) competitively inhibited the uptake of characteristic substrates p-aminohippurate for hOAT1 and estrone sulfate for hOAT3. p-Aminohippuric Acid 107-123 solute carrier family 22 member 6 Homo sapiens 128-133 16724555-3 2006 Zalcitabine exhibited the inhibition effect on the cellular uptake of [3H]-PAH in CHO/hOAT1 cells with an IC50 value of 1.23 mM. p-Aminohippuric Acid 75-78 solute carrier family 22 member 6 Homo sapiens 86-91 16997449-2 2006 p-Aminohippurate uptake exhibited similar kinetics as published values, was inhibited by cephaloridine, cimetidine, methotrexate, and urate, consistent with function of both organic anion transporter 1 (OAT1) and OAT3. p-Aminohippuric Acid 0-16 solute carrier family 22 member 6 Homo sapiens 174-201 16997449-2 2006 p-Aminohippurate uptake exhibited similar kinetics as published values, was inhibited by cephaloridine, cimetidine, methotrexate, and urate, consistent with function of both organic anion transporter 1 (OAT1) and OAT3. p-Aminohippuric Acid 0-16 solute carrier family 22 member 6 Homo sapiens 203-207 15870380-7 2005 The uptake of the prototype OAT substrate PAH in hOAT1-expressing oocytes was dose dependently and potently inhibited by EA with an IC(50)of 207 nM. p-Aminohippuric Acid 42-45 solute carrier family 22 member 6 Homo sapiens 49-54 15644426-5 2005 In contrast, steviol showed significant, dose-dependent inhibition of PAH and ES uptake in hOAT1- or hOAT3-expressing oocytes, respectively. p-Aminohippuric Acid 70-73 solute carrier family 22 member 6 Homo sapiens 91-96 15914676-6 2005 When expressed in Xenopus oocytes, wild-type R50-hOAT1 and the variants R50H-hOAT1 and K525I-hOAT1 all mediated the probenecid-sensitive uptake of the classic organic anion para-aminohippurate (PAH). p-Aminohippuric Acid 173-192 solute carrier family 22 member 6 Homo sapiens 49-54 15914676-6 2005 When expressed in Xenopus oocytes, wild-type R50-hOAT1 and the variants R50H-hOAT1 and K525I-hOAT1 all mediated the probenecid-sensitive uptake of the classic organic anion para-aminohippurate (PAH). p-Aminohippuric Acid 173-192 solute carrier family 22 member 6 Homo sapiens 77-82 15914676-6 2005 When expressed in Xenopus oocytes, wild-type R50-hOAT1 and the variants R50H-hOAT1 and K525I-hOAT1 all mediated the probenecid-sensitive uptake of the classic organic anion para-aminohippurate (PAH). p-Aminohippuric Acid 173-192 solute carrier family 22 member 6 Homo sapiens 77-82 15914676-6 2005 When expressed in Xenopus oocytes, wild-type R50-hOAT1 and the variants R50H-hOAT1 and K525I-hOAT1 all mediated the probenecid-sensitive uptake of the classic organic anion para-aminohippurate (PAH). p-Aminohippuric Acid 194-197 solute carrier family 22 member 6 Homo sapiens 49-54 15914676-6 2005 When expressed in Xenopus oocytes, wild-type R50-hOAT1 and the variants R50H-hOAT1 and K525I-hOAT1 all mediated the probenecid-sensitive uptake of the classic organic anion para-aminohippurate (PAH). p-Aminohippuric Acid 194-197 solute carrier family 22 member 6 Homo sapiens 77-82 15914676-6 2005 When expressed in Xenopus oocytes, wild-type R50-hOAT1 and the variants R50H-hOAT1 and K525I-hOAT1 all mediated the probenecid-sensitive uptake of the classic organic anion para-aminohippurate (PAH). p-Aminohippuric Acid 194-197 solute carrier family 22 member 6 Homo sapiens 77-82 15644426-6 2005 The IC(50) of steviol for hOAT1-mediated PAH transport was 11.1 microM compared with 62.6 microM for hOAT3-mediated ES uptake. p-Aminohippuric Acid 41-44 solute carrier family 22 member 6 Homo sapiens 26-31 15039295-10 2004 Further functional comparisons of hOAT1-1 and hOAT1-2 in fluorescein uptake studies exhibited almost identical affinities for fluorescein with Michaelis constants of 11.6 +/- 3.7 microM (hOAT1-1) and 11.9 +/- 6.4 microM (hOAT1-2), and similar sensitivities to inhibition by p-aminohippurate [IC(50): 16 microM (hOAT1-1), 10 microM (hOAT1-2)], urate [IC(50): 440 microM (hOAT1-1), 385 microM (hOAT1-2)], and furosemide (IC(50): 14 microM (hOAT1-1), 20 microM (hOAT1-2)], implying functional equivalence. p-Aminohippuric Acid 274-290 solute carrier family 22 member 6 Homo sapiens 34-39 15039295-10 2004 Further functional comparisons of hOAT1-1 and hOAT1-2 in fluorescein uptake studies exhibited almost identical affinities for fluorescein with Michaelis constants of 11.6 +/- 3.7 microM (hOAT1-1) and 11.9 +/- 6.4 microM (hOAT1-2), and similar sensitivities to inhibition by p-aminohippurate [IC(50): 16 microM (hOAT1-1), 10 microM (hOAT1-2)], urate [IC(50): 440 microM (hOAT1-1), 385 microM (hOAT1-2)], and furosemide (IC(50): 14 microM (hOAT1-1), 20 microM (hOAT1-2)], implying functional equivalence. p-Aminohippuric Acid 274-290 solute carrier family 22 member 6 Homo sapiens 46-51 15039295-10 2004 Further functional comparisons of hOAT1-1 and hOAT1-2 in fluorescein uptake studies exhibited almost identical affinities for fluorescein with Michaelis constants of 11.6 +/- 3.7 microM (hOAT1-1) and 11.9 +/- 6.4 microM (hOAT1-2), and similar sensitivities to inhibition by p-aminohippurate [IC(50): 16 microM (hOAT1-1), 10 microM (hOAT1-2)], urate [IC(50): 440 microM (hOAT1-1), 385 microM (hOAT1-2)], and furosemide (IC(50): 14 microM (hOAT1-1), 20 microM (hOAT1-2)], implying functional equivalence. p-Aminohippuric Acid 274-290 solute carrier family 22 member 6 Homo sapiens 46-51 15039295-10 2004 Further functional comparisons of hOAT1-1 and hOAT1-2 in fluorescein uptake studies exhibited almost identical affinities for fluorescein with Michaelis constants of 11.6 +/- 3.7 microM (hOAT1-1) and 11.9 +/- 6.4 microM (hOAT1-2), and similar sensitivities to inhibition by p-aminohippurate [IC(50): 16 microM (hOAT1-1), 10 microM (hOAT1-2)], urate [IC(50): 440 microM (hOAT1-1), 385 microM (hOAT1-2)], and furosemide (IC(50): 14 microM (hOAT1-1), 20 microM (hOAT1-2)], implying functional equivalence. p-Aminohippuric Acid 274-290 solute carrier family 22 member 6 Homo sapiens 46-51 15039295-10 2004 Further functional comparisons of hOAT1-1 and hOAT1-2 in fluorescein uptake studies exhibited almost identical affinities for fluorescein with Michaelis constants of 11.6 +/- 3.7 microM (hOAT1-1) and 11.9 +/- 6.4 microM (hOAT1-2), and similar sensitivities to inhibition by p-aminohippurate [IC(50): 16 microM (hOAT1-1), 10 microM (hOAT1-2)], urate [IC(50): 440 microM (hOAT1-1), 385 microM (hOAT1-2)], and furosemide (IC(50): 14 microM (hOAT1-1), 20 microM (hOAT1-2)], implying functional equivalence. p-Aminohippuric Acid 274-290 solute carrier family 22 member 6 Homo sapiens 46-51 15039295-10 2004 Further functional comparisons of hOAT1-1 and hOAT1-2 in fluorescein uptake studies exhibited almost identical affinities for fluorescein with Michaelis constants of 11.6 +/- 3.7 microM (hOAT1-1) and 11.9 +/- 6.4 microM (hOAT1-2), and similar sensitivities to inhibition by p-aminohippurate [IC(50): 16 microM (hOAT1-1), 10 microM (hOAT1-2)], urate [IC(50): 440 microM (hOAT1-1), 385 microM (hOAT1-2)], and furosemide (IC(50): 14 microM (hOAT1-1), 20 microM (hOAT1-2)], implying functional equivalence. p-Aminohippuric Acid 274-290 solute carrier family 22 member 6 Homo sapiens 46-51 15039295-10 2004 Further functional comparisons of hOAT1-1 and hOAT1-2 in fluorescein uptake studies exhibited almost identical affinities for fluorescein with Michaelis constants of 11.6 +/- 3.7 microM (hOAT1-1) and 11.9 +/- 6.4 microM (hOAT1-2), and similar sensitivities to inhibition by p-aminohippurate [IC(50): 16 microM (hOAT1-1), 10 microM (hOAT1-2)], urate [IC(50): 440 microM (hOAT1-1), 385 microM (hOAT1-2)], and furosemide (IC(50): 14 microM (hOAT1-1), 20 microM (hOAT1-2)], implying functional equivalence. p-Aminohippuric Acid 274-290 solute carrier family 22 member 6 Homo sapiens 46-51 15039295-10 2004 Further functional comparisons of hOAT1-1 and hOAT1-2 in fluorescein uptake studies exhibited almost identical affinities for fluorescein with Michaelis constants of 11.6 +/- 3.7 microM (hOAT1-1) and 11.9 +/- 6.4 microM (hOAT1-2), and similar sensitivities to inhibition by p-aminohippurate [IC(50): 16 microM (hOAT1-1), 10 microM (hOAT1-2)], urate [IC(50): 440 microM (hOAT1-1), 385 microM (hOAT1-2)], and furosemide (IC(50): 14 microM (hOAT1-1), 20 microM (hOAT1-2)], implying functional equivalence. p-Aminohippuric Acid 274-290 solute carrier family 22 member 6 Homo sapiens 46-51 15039295-10 2004 Further functional comparisons of hOAT1-1 and hOAT1-2 in fluorescein uptake studies exhibited almost identical affinities for fluorescein with Michaelis constants of 11.6 +/- 3.7 microM (hOAT1-1) and 11.9 +/- 6.4 microM (hOAT1-2), and similar sensitivities to inhibition by p-aminohippurate [IC(50): 16 microM (hOAT1-1), 10 microM (hOAT1-2)], urate [IC(50): 440 microM (hOAT1-1), 385 microM (hOAT1-2)], and furosemide (IC(50): 14 microM (hOAT1-1), 20 microM (hOAT1-2)], implying functional equivalence. p-Aminohippuric Acid 274-290 solute carrier family 22 member 6 Homo sapiens 46-51 15039295-10 2004 Further functional comparisons of hOAT1-1 and hOAT1-2 in fluorescein uptake studies exhibited almost identical affinities for fluorescein with Michaelis constants of 11.6 +/- 3.7 microM (hOAT1-1) and 11.9 +/- 6.4 microM (hOAT1-2), and similar sensitivities to inhibition by p-aminohippurate [IC(50): 16 microM (hOAT1-1), 10 microM (hOAT1-2)], urate [IC(50): 440 microM (hOAT1-1), 385 microM (hOAT1-2)], and furosemide (IC(50): 14 microM (hOAT1-1), 20 microM (hOAT1-2)], implying functional equivalence. p-Aminohippuric Acid 274-290 solute carrier family 22 member 6 Homo sapiens 46-51 12606766-6 2003 The cytotoxic effects were fully reversed by probenecid (an OAT1 inhibitor) and partially reversed by p-aminohippurate (an OAT1 substrate). p-Aminohippuric Acid 102-118 solute carrier family 22 member 6 Homo sapiens 123-127 12874449-3 2003 Stimulation of PKC with sn-1,2-dioctanoylglycerol resulted in strong inhibition of p-aminohippurate transport mediated by the cloned human organic anion transporter 1 (hOAT1) expressed in Xenopus oocytes and HEK293 cells, as well as hOAT1 internalization in both expression systems. p-Aminohippuric Acid 83-99 solute carrier family 22 member 6 Homo sapiens 139-166 12874449-3 2003 Stimulation of PKC with sn-1,2-dioctanoylglycerol resulted in strong inhibition of p-aminohippurate transport mediated by the cloned human organic anion transporter 1 (hOAT1) expressed in Xenopus oocytes and HEK293 cells, as well as hOAT1 internalization in both expression systems. p-Aminohippuric Acid 83-99 solute carrier family 22 member 6 Homo sapiens 168-173 12874449-3 2003 Stimulation of PKC with sn-1,2-dioctanoylglycerol resulted in strong inhibition of p-aminohippurate transport mediated by the cloned human organic anion transporter 1 (hOAT1) expressed in Xenopus oocytes and HEK293 cells, as well as hOAT1 internalization in both expression systems. p-Aminohippuric Acid 83-99 solute carrier family 22 member 6 Homo sapiens 233-238 12874449-5 2003 It was independent of the conserved canonical PKC consensus sites in hOAT1, however, and was unaffected by agents that destabilize actin filaments or microtubules, which altered baseline hOAT1-mediated p-aminohippurate uptake activity in oocytes. p-Aminohippuric Acid 204-218 solute carrier family 22 member 6 Homo sapiens 187-192 11744606-10 2002 In contrast, probenecid effectively inhibited the transport of PAH, Ro 64-0802, and amoxicillin via hOAT1. p-Aminohippuric Acid 63-66 solute carrier family 22 member 6 Homo sapiens 100-105 12538807-6 2003 Finally, preloading cells transfected with hOAT1 with BTC significantly trans-stimulated the uptake of PAH, consistent with the conclusion that BTC and, hence, other cysteine S-conjugates are substrates for hOAT1. p-Aminohippuric Acid 103-106 solute carrier family 22 member 6 Homo sapiens 43-48 12538807-6 2003 Finally, preloading cells transfected with hOAT1 with BTC significantly trans-stimulated the uptake of PAH, consistent with the conclusion that BTC and, hence, other cysteine S-conjugates are substrates for hOAT1. p-Aminohippuric Acid 103-106 solute carrier family 22 member 6 Homo sapiens 207-212 11744606-8 2002 Ro 64-0802 was found to be a low-efficiency substrate for hOAT1 and a very weak inhibitor of hOAT1-mediated transport of p-aminohippuric acid (PAH). p-Aminohippuric Acid 121-141 solute carrier family 22 member 6 Homo sapiens 93-98 11744606-8 2002 Ro 64-0802 was found to be a low-efficiency substrate for hOAT1 and a very weak inhibitor of hOAT1-mediated transport of p-aminohippuric acid (PAH). p-Aminohippuric Acid 143-146 solute carrier family 22 member 6 Homo sapiens 93-98 12538807-3 2003 Since the OAT1 transporter has the characteristics of para-aminokippurate (PAH) transport that closely correlate to the native RPT, we examined the interaction of DCVC, CTFC, and the nontoxic benzothiazolylcysteine (BTC) with PAH transport mediated by human OAT1 and rabbit Oat1 expressed in Chinese hamster ovary and COS7 heterologous expression systems, respectively. p-Aminohippuric Acid 75-78 solute carrier family 22 member 6 Homo sapiens 10-14 12538807-3 2003 Since the OAT1 transporter has the characteristics of para-aminokippurate (PAH) transport that closely correlate to the native RPT, we examined the interaction of DCVC, CTFC, and the nontoxic benzothiazolylcysteine (BTC) with PAH transport mediated by human OAT1 and rabbit Oat1 expressed in Chinese hamster ovary and COS7 heterologous expression systems, respectively. p-Aminohippuric Acid 226-229 solute carrier family 22 member 6 Homo sapiens 10-14 12538807-4 2003 Although the K(m) values for PAH uptake by hOAT1 and rbOat1 (8.9 +/- 3.6 and 20.7 +/- 8 microM, respectively) were 5- to 10-fold less than the K(m) for peritubular PAH transport into rabbit RPT, the IC(50) values for DCVC, CTFC, and BTC inhibition of PAH uptake mediated by either hOAT1 or rbOat1 were similar between these two transporters and to the IC(50) values for these conjugates measured in rabbit RPT. p-Aminohippuric Acid 29-32 solute carrier family 22 member 6 Homo sapiens 43-48 12538807-4 2003 Although the K(m) values for PAH uptake by hOAT1 and rbOat1 (8.9 +/- 3.6 and 20.7 +/- 8 microM, respectively) were 5- to 10-fold less than the K(m) for peritubular PAH transport into rabbit RPT, the IC(50) values for DCVC, CTFC, and BTC inhibition of PAH uptake mediated by either hOAT1 or rbOat1 were similar between these two transporters and to the IC(50) values for these conjugates measured in rabbit RPT. p-Aminohippuric Acid 29-32 solute carrier family 22 member 6 Homo sapiens 281-286 12538807-4 2003 Although the K(m) values for PAH uptake by hOAT1 and rbOat1 (8.9 +/- 3.6 and 20.7 +/- 8 microM, respectively) were 5- to 10-fold less than the K(m) for peritubular PAH transport into rabbit RPT, the IC(50) values for DCVC, CTFC, and BTC inhibition of PAH uptake mediated by either hOAT1 or rbOat1 were similar between these two transporters and to the IC(50) values for these conjugates measured in rabbit RPT. p-Aminohippuric Acid 164-167 solute carrier family 22 member 6 Homo sapiens 43-48 12538807-4 2003 Although the K(m) values for PAH uptake by hOAT1 and rbOat1 (8.9 +/- 3.6 and 20.7 +/- 8 microM, respectively) were 5- to 10-fold less than the K(m) for peritubular PAH transport into rabbit RPT, the IC(50) values for DCVC, CTFC, and BTC inhibition of PAH uptake mediated by either hOAT1 or rbOat1 were similar between these two transporters and to the IC(50) values for these conjugates measured in rabbit RPT. p-Aminohippuric Acid 164-167 solute carrier family 22 member 6 Homo sapiens 43-48 12538807-5 2003 The IC(50) for inhibition of hOAT1- and rbOat1-mediated PAH uptake by the hydrophobic conjugate BTC was more than 5-fold lower than the IC(50) values seen with DCVC and CTFC, suggesting that hydrophobicity increases the affinity of OAT1 for cysteine conjugates. p-Aminohippuric Acid 56-59 solute carrier family 22 member 6 Homo sapiens 29-34 12605306-8 2003 OAT1 translocated, e.g., anti-inflammatory drugs, antiviral drugs, beta-lactam antibiotics, loop diuretics, ochratoxin A, and p-aminohippurate. p-Aminohippuric Acid 126-142 solute carrier family 22 member 6 Homo sapiens 0-4 9887087-4 1999 When expressed in Xenopus laevis oocytes, hOAT1 mediated sodium-independent uptake of p-aminohippurate (PAH) (Km = 9. p-Aminohippuric Acid 86-102 solute carrier family 22 member 6 Homo sapiens 42-47 9887087-4 1999 When expressed in Xenopus laevis oocytes, hOAT1 mediated sodium-independent uptake of p-aminohippurate (PAH) (Km = 9. p-Aminohippuric Acid 104-107 solute carrier family 22 member 6 Homo sapiens 42-47 9887087-6 1999 hOAT1-mediated PAH uptake was inhibited by bulky inorganic anions, various xenobiotics, and endogenous substances, including benzylpenicillin, furosemide, indomethacin, probenecid, phenol red, urate, and alpha-ketoglutarate. p-Aminohippuric Acid 15-18 solute carrier family 22 member 6 Homo sapiens 0-5 35508593-6 2022 Additionally, a relationship was derived between the estimated glomerular filtration rate and the reduction in OAT1/3-mediated secretion of drugs based on the renal extraction ratios of -aminohippuric acid in patients with varying degrees of renal impairment. p-Aminohippuric Acid 187-206 solute carrier family 22 member 6 Homo sapiens 111-117 11602689-6 2001 [3H]PAH was used to show the transport activity of hOAT1 (Km = 3.9 +/-1.3 microM). p-Aminohippuric Acid 4-7 solute carrier family 22 member 6 Homo sapiens 51-56 11443229-2 2001 Uptake of PAH from blood into tubule cells occurs by exchange with intracellular alpha-ketoglutarate and is mediated by the organic anion transporter 1. p-Aminohippuric Acid 10-13 solute carrier family 22 member 6 Homo sapiens 124-151 11327718-3 2001 hOAT1- and hOAT3-transfected cells showed uptake of p-aminohippurate and fluorescein. p-Aminohippuric Acid 52-68 solute carrier family 22 member 6 Homo sapiens 0-5 10049739-6 1999 PAH uptake by Xenopus laevis oocytes injected with hOAT1 mRNA is increased 100-fold compared to water-injected oocytes. p-Aminohippuric Acid 0-3 solute carrier family 22 member 6 Homo sapiens 51-56 22988478-9 2012 Among all the 63 CHMs, formulae Gui Zhi Fu Ling Wan (GZ) and Chia Wei Hsiao Yao San (CW) exhibited significant inhibitions on hOAT1-mediated [(3)H]-PAH uptake in vitro and PAH clearance and net secretion in vivo. p-Aminohippuric Acid 148-151 solute carrier family 22 member 6 Homo sapiens 126-131 22988478-9 2012 Among all the 63 CHMs, formulae Gui Zhi Fu Ling Wan (GZ) and Chia Wei Hsiao Yao San (CW) exhibited significant inhibitions on hOAT1-mediated [(3)H]-PAH uptake in vitro and PAH clearance and net secretion in vivo. p-Aminohippuric Acid 172-175 solute carrier family 22 member 6 Homo sapiens 126-131