PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 22776178-3 2012 Thrombin concentration was measured by fluorescence microscopy and spectroscopy, in which aptamers were labeled with either fluorescein dye or quantum dots. Fluorescein 124-135 coagulation factor II, thrombin Homo sapiens 0-8 27522482-1 2017 In this work, we developed three fluorescent probes (F-1, F-2, and F-3) based on fluorescein, mono-formylated fluorescein, and bis-formylated fluorescein for hydrogen sulfide (H2S) detection. Fluorescein 81-92 coagulation factor II, thrombin Homo sapiens 58-61 24508546-4 2014 When the other thrombin aptamer labeled with fluorescein (5"-FAM-AGTCCGTGGTAGGGCAGGTTGGGGTGACT-3") was added to the above system, a sandwich structure can form at the surface of silica nanoparticles. Fluorescein 45-56 coagulation factor II, thrombin Homo sapiens 15-23 11278943-0 2001 The use of prothrombin(S525C) labeled with fluorescein to directly study the inhibition of prothrombinase by antithrombin during prothrombin activation. Fluorescein 43-54 coagulation factor II, thrombin Homo sapiens 11-22 16843700-8 2006 In addition, ecotin modulated the enzyme"s catalytic site, as demonstrated by changes in the fluorescence emission of fluorescein-FPRCK-alpha-thrombin (EC50=3.5 microM). Fluorescein 118-129 coagulation factor II, thrombin Homo sapiens 142-150 16241952-1 2005 Binding of fluorescein-labeled coagulation factors IXa, VIII, X, and allophycocyanin-labeled annexin V to thrombin-activated platelets was studied using flow cytometry. Fluorescein 11-22 coagulation factor II, thrombin Homo sapiens 106-114 12659199-8 2003 In this proof-of-principle work, the NECEEM method was applied to the analysis of thrombin using a fluorescein-labeled aptamer under the conditions at which the protein-aptamer complex completely decayed during the separation. Fluorescein 99-110 coagulation factor II, thrombin Homo sapiens 82-90 11514680-5 2001 Gel filtration chromatography demonstrated calcium-independent, 1:1 complex formation between fluorescein-labeled BJC ([5F]BJC) and prothrombin, whereas no interactions were observed with activation fragments 1 or 2 of prothrombin. Fluorescein 94-105 coagulation factor II, thrombin Homo sapiens 132-143 19785411-3 2009 As a result of specific protein/aptamer interaction, the presence of thrombin induces the primary aptamer-functionalized NPs to form a sandwich structure with fluorescein-labeled secondary aptamer and results in fluorescent NPs. Fluorescein 159-170 coagulation factor II, thrombin Homo sapiens 69-77 19640005-9 2009 Further studies with the fluorescein labeled Cys-195 mutant of thrombin revealed that the catalytic residue of thrombin is modulated by Na(+), but TM has no effect on the conformation of this residue. Fluorescein 25-36 coagulation factor II, thrombin Homo sapiens 63-71 19640005-9 2009 Further studies with the fluorescein labeled Cys-195 mutant of thrombin revealed that the catalytic residue of thrombin is modulated by Na(+), but TM has no effect on the conformation of this residue. Fluorescein 25-36 coagulation factor II, thrombin Homo sapiens 111-119 17726029-11 2007 Initial rates of fluorescein-labeled prothrombin cleavage in the presence of various prothrombin derivatives reported by Brufatto and Nesheim (Brufatto, N., and Nesheim, M. E. (2003) J. Biol. Fluorescein 17-28 coagulation factor II, thrombin Homo sapiens 37-48 17726029-11 2007 Initial rates of fluorescein-labeled prothrombin cleavage in the presence of various prothrombin derivatives reported by Brufatto and Nesheim (Brufatto, N., and Nesheim, M. E. (2003) J. Biol. Fluorescein 17-28 coagulation factor II, thrombin Homo sapiens 85-96 12724354-5 2003 An effect on the fibrinolysis pathway after treatment of HUVECs with thrombin was shown by increased fluorescein-labeled plasminogen binding to cells, which was inhibited by an antibody specific for p11. Fluorescein 101-112 coagulation factor II, thrombin Homo sapiens 69-77 12871328-3 2003 Experiments were carried out using a recombinant form of prothrombin in which the active site serine has been mutated to cysteine and subsequently labeled with fluorescein. Fluorescein 160-171 coagulation factor II, thrombin Homo sapiens 57-68 11278943-0 2001 The use of prothrombin(S525C) labeled with fluorescein to directly study the inhibition of prothrombinase by antithrombin during prothrombin activation. Fluorescein 43-54 coagulation factor II, thrombin Homo sapiens 91-102 11278943-1 2001 Serine 525 of human prothrombin was mutated to cysteine and covalently labeled with fluorescein to make II(S525C)-fluorescein. Fluorescein 84-95 coagulation factor II, thrombin Homo sapiens 20-31 11278943-1 2001 Serine 525 of human prothrombin was mutated to cysteine and covalently labeled with fluorescein to make II(S525C)-fluorescein. Fluorescein 114-125 coagulation factor II, thrombin Homo sapiens 20-31 29136862-5 2018 Upon the introduction of thrombin, thrombin specifically recognized its aptamer, so aptamer released from the MSN; and the single strand DNA(ssDNA) left could not cap the pores of the MSN efficiently and hence caused the releasing of fluorescein into the solution. Fluorescein 234-245 coagulation factor II, thrombin Homo sapiens 25-33 10860511-4 2000 The imaging fiber was coupled to a modified epifluorescence microscope system, and the distal end of the fiber was incubated with a fluorescein-labeled thrombin (F-thrombin) solution. Fluorescein 132-143 coagulation factor II, thrombin Homo sapiens 152-160 10860511-4 2000 The imaging fiber was coupled to a modified epifluorescence microscope system, and the distal end of the fiber was incubated with a fluorescein-labeled thrombin (F-thrombin) solution. Fluorescein 132-143 coagulation factor II, thrombin Homo sapiens 164-172 10378842-12 1999 Fluorescein was also shown to be released during aggregation with thrombin or ADP but not during agglutination with ristocetin. Fluorescein 0-11 coagulation factor II, thrombin Homo sapiens 66-74 9325262-3 1997 Binding studies were performed in solution with fluorescein-Phe-Pro-Arg-CH2Cl (FPR)-thrombin. Fluorescein 48-59 coagulation factor II, thrombin Homo sapiens 84-92 8111066-7 1994 Quantitative fluorescence microscopy, using fluorescein-labeled fibrin, showed that fibrin adherent to the surface of thrombin-stimulated platelets lysed 20% to 50% more slowly than bulk fibrin (monitored in parallel by laser light scattering). Fluorescein 44-55 coagulation factor II, thrombin Homo sapiens 118-126 2302458-4 1990 Quantitative fluorescence microscopy (microfluorimetry) has been used to determine the quantity of fluorescein-labeled fibrin bound to the surface of thrombin-stimulated, gel-filtered platelets (the supernatants of which contained small quantities of vWF) in the presence/absence of receptor-specific and vWF-specific monoclonal antibodies (MoAbs), as well as exogenous vWF. Fluorescein 99-110 coagulation factor II, thrombin Homo sapiens 150-158 3219359-8 1988 Incubation of N alpha-[(acetylthio)acetyl]-D-Phe-Pro-Arg-thrombin with 5-(iodoacetamido)fluorescein in the presence of NH2OH resulted in incorporation of 0.96 mol of the fluorescence probe/mol of active sites and the appearance of fluorescein fluorescence associated with the active site containing B-chain on sodium dodecyl sulfate-polyacrylamide gels. Fluorescein 88-99 coagulation factor II, thrombin Homo sapiens 57-65 7318826-5 1981 These show that stimulation with thrombin also induces a rapid change in the platelet transmembrane pH gradient as measured using either a weak base or a fluorescein derivative as a probe. Fluorescein 154-165 coagulation factor II, thrombin Homo sapiens 33-41 9856996-6 1998 Fluorescein-labeled heparin was displaced from either thrombin or active site blocked thrombin by ATH, indicating that thrombin must bind to the conjugate"s heparin moiety. Fluorescein 0-11 coagulation factor II, thrombin Homo sapiens 54-62 9856996-6 1998 Fluorescein-labeled heparin was displaced from either thrombin or active site blocked thrombin by ATH, indicating that thrombin must bind to the conjugate"s heparin moiety. Fluorescein 0-11 coagulation factor II, thrombin Homo sapiens 86-94 9856996-6 1998 Fluorescein-labeled heparin was displaced from either thrombin or active site blocked thrombin by ATH, indicating that thrombin must bind to the conjugate"s heparin moiety. Fluorescein 0-11 coagulation factor II, thrombin Homo sapiens 86-94 1317850-3 1992 Hirugen was a competitive inhibitor for either GF1-6 or GF2.5-6 stimulation of thrombin activation of protein C. GF5-6, which binds to thrombin without altering its ability to activate protein C, competed with fluorescein-labeled Hirugen for binding to thrombin. Fluorescein 210-221 coagulation factor II, thrombin Homo sapiens 79-87 1660464-9 1991 The environments of the fluorescein and ANS dyes were also altered when GF1-6 bound to thrombin because binding resulted in emission intensity changes of -13% and +18%, respectively. Fluorescein 24-35 coagulation factor II, thrombin Homo sapiens 87-95 2524476-5 1989 Prothrombin and factor Xa both competed with fluorescein-labeled PAP-I for binding to anionic phospholipid vesicles, but with affinities at least 1000-fold weaker than PAP-I. Fluorescein 45-56 coagulation factor II, thrombin Homo sapiens 0-11 3434788-3 1987 We report here a modification of the previously published 6-CF technique and confirm the thrombin-induced cytoplasmic pH change in the human platelet using a second fluorescein derivative, BCECF. Fluorescein 165-176 coagulation factor II, thrombin Homo sapiens 89-97 6439669-0 1984 Origin of a fluorescence increase accompanying the limited proteolysis of fluorescein-labeled human prothrombin by Factor Xa. Fluorescein 74-85 coagulation factor II, thrombin Homo sapiens 100-111 6439669-1 1984 In a search for a probe which would report its proteolysis to thrombin, the human blood coagulation zymogen prothrombin was covalently labeled with fluorescein. Fluorescein 148-159 coagulation factor II, thrombin Homo sapiens 62-70 6439669-1 1984 In a search for a probe which would report its proteolysis to thrombin, the human blood coagulation zymogen prothrombin was covalently labeled with fluorescein. Fluorescein 148-159 coagulation factor II, thrombin Homo sapiens 108-119 6696918-1 1984 Derivatives of human thrombin and antithrombin III with fluorescent labels covalently attached to their carbohydrate moieties were prepared by reaction of periodate-oxidized proteins with amino derivatives of dansyl, fluorescein and pyrene. Fluorescein 217-228 coagulation factor II, thrombin Homo sapiens 21-29 29136862-5 2018 Upon the introduction of thrombin, thrombin specifically recognized its aptamer, so aptamer released from the MSN; and the single strand DNA(ssDNA) left could not cap the pores of the MSN efficiently and hence caused the releasing of fluorescein into the solution. Fluorescein 234-245 coagulation factor II, thrombin Homo sapiens 35-43 28994575-5 2017 Fluorescein-labeled ACPP was used to evaluate the efficiency of thrombin-triggered cleavage by measuring the mean fluorescence intensity of ACPP-labeled MBs (ACPP-MBs) before and after incubation at 37 C with thrombin. Fluorescein 0-11 coagulation factor II, thrombin Homo sapiens 64-72 28994575-5 2017 Fluorescein-labeled ACPP was used to evaluate the efficiency of thrombin-triggered cleavage by measuring the mean fluorescence intensity of ACPP-labeled MBs (ACPP-MBs) before and after incubation at 37 C with thrombin. Fluorescein 0-11 coagulation factor II, thrombin Homo sapiens 210-218