PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 2110000-2 1990 The effects of GDP and of aurodox (N-methylkirromycin) on the affinity of elongation factor Tu (EF-Tu) for aminoacyl-tRNA (aa-tRNA) have been quantified spectroscopically by using Phe-tRNA(Phe)-Fl8, a functionally active analogue of Phe-tRNA(Phe) with a fluorescein dye convalently attached to the s4U-8 base. Fluorescein 254-265 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 74-94 7794902-5 1995 Formation of the Phe-tRNAPhe-Fl8.EF-Tu.GTP-Rh ternary complex was verified both by EF-Tu protection of the aminoacyl bond from chemical hydrolysis and by an EF-Tu.GTP-dependent increase in fluorescein intensity. Fluorescein 189-200 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 33-38 7794902-6 1995 Spectral analyses revealed that both the emission intensity and lifetime of fluorescein were greater in the Phe-tRNAPhe-Fl8.EF-Tu.GTP ternary complex than in the Phe-tRNAPhe-Fl8.EF-Tu.GTP-Rh ternary complex. Fluorescein 76-87 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 124-129 7794902-6 1995 Spectral analyses revealed that both the emission intensity and lifetime of fluorescein were greater in the Phe-tRNAPhe-Fl8.EF-Tu.GTP ternary complex than in the Phe-tRNAPhe-Fl8.EF-Tu.GTP-Rh ternary complex. Fluorescein 76-87 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 178-183 2190631-8 1990 Since an EF-Tu-dependent fluorescence change was also observed with fluorescein-labeled tRNA(Phe), the protein-dependent structural change is effected by direct interactions between EF-Tu and the tRNA and does not require the aminoacyl group. Fluorescein 68-79 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 9-14 2190631-8 1990 Since an EF-Tu-dependent fluorescence change was also observed with fluorescein-labeled tRNA(Phe), the protein-dependent structural change is effected by direct interactions between EF-Tu and the tRNA and does not require the aminoacyl group. Fluorescein 68-79 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 182-187 2110000-2 1990 The effects of GDP and of aurodox (N-methylkirromycin) on the affinity of elongation factor Tu (EF-Tu) for aminoacyl-tRNA (aa-tRNA) have been quantified spectroscopically by using Phe-tRNA(Phe)-Fl8, a functionally active analogue of Phe-tRNA(Phe) with a fluorescein dye convalently attached to the s4U-8 base. Fluorescein 254-265 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 96-101 2110000-3 1990 The association of EF-Tu.GDP with Phe-tRNA(Phe)-Fl8 resulted in an average increase of 33% in fluorescein emission intensity. Fluorescein 94-105 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 19-24