PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 32371236-2 2020 We utilized this technique to generate a self-cloned wine yeast strain that overexpresses two genes of oenological relevance i.e. the glycerol-3-phosphate dehydrogenase 1 (GPD1) and the alcohol acetyltransferase 1 (ATF1) directly implicated in glycerol and acetate ester production respectively. Glycerol 134-142 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 172-176 30488283-3 2019 Deletion of structural genes like gpd1and gpd2 leads to an increase in ethanol by reducing glycerol; however, it makes the yeast osmosensitive that is not desirable for industrial strains. Glycerol 91-99 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 34-38 31395335-0 2019 Ste20 and Cla4 modulate the expression of the glycerol biosynthesis enzyme Gpd1 by a novel MAPK-independent pathway. Glycerol 46-54 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 75-79 31395335-5 2019 Here, we show that Ste20 and Cla4 not only stimulate transcription, they also bind to the glycerol biosynthesis enzymes Gpd1, Gpp1 and Gpp2. Glycerol 90-98 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 120-124 31395335-6 2019 Protein levels of Gpd1, the enzyme that catalyzes the rate limiting step in glycerol synthesis, positively correlate with glucose availability. Glycerol 76-84 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 18-22 30978585-4 2019 Based on these results, the yeast GPD1 gene was replaced with the endogenous gpsA gene to modulate the rate of glycerol metabolism. Glycerol 111-119 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 34-38 30687253-9 2018 In both cases, heteroresistance in S. cerevisiae was strikingly dependent on the GPD1 gene product, important for glycerol synthesis. Glycerol 114-122 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 81-85 30687253-12 2018 The study reports diverse osmotolerance and halotolerance phenotypes and heteroresistance across an extensive panel of yeast isolates, and indicates that Gpd1-dependent glycerol synthesis is a key determinant enabling growth of rare yeast subpopulations at low Aw, brought about by glucose and in particular salt. Glycerol 169-177 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 154-158 30018610-0 2018 GPD1 and ADH3 Natural Variants Underlie Glycerol Yield Differences in Wine Fermentation. Glycerol 40-48 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 0-4 30018610-10 2018 In this line, Gpd1 protein levels in parental strains, particularly Gpd1pWE, did not necessarily correlate with gene expression differences, but rather with glycerol yield where low Gpd1pWE levels were detected. Glycerol 157-165 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 14-18 30018610-12 2018 This dissection of regulatory mechanisms in GPD1 allelic variants demonstrates the potential to exploit natural alleles to improve glycerol production in wine fermentation and highlights the difficulties of trait improvement due to alternative trans-regulation and gene-gene interactions in the different genetic background. Glycerol 131-139 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 44-48 30018610-6 2018 A reciprocal hemizygosity assay demonstrated that hemizygotes expressing GPD1WA , GPD1SA , ADH3WA and ADH3SA alleles had significantly greater glycerol yields compared to GPD1WE and ADH3WE . Glycerol 143-151 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 73-77 28280870-8 2017 To increase glycerol production and accumulation from glucose, we lowered the expression of TPI1 gene coding for triose phosphate isomerase; overexpressed the fused gene consisting the GPD1 and GPP2 parts coding for glycerol-3-phosphate dehydrogenase and glycerol-3-phosphate phosphatase, respectively; overexpressed the engineered FPS1 gene that codes for aquaglyceroporin; and overexpressed the truncated gene ILV2 that codes for acetolactate synthase. Glycerol 12-20 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 185-189 26164272-4 2016 We show that the glycerol synthetic enzyme Gpd1 is involved in the post-translational modification of Tcp-1 (subunit of TRiC) by acetylation and glycation through the NAD(+)/NADH shuttle and the triose phosphate intermediate dihydroxyacetone phosphate, respectively. Glycerol 17-25 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 43-47 26846624-0 2016 RNA binding protein Pub1p regulates glycerol production and stress tolerance by controlling Gpd1p activity during winemaking. Glycerol 36-44 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 92-97 26846624-4 2016 Glycerol is produced by diverting glycolytic glyceraldehyde-3-phosphate through the action of glycerol-3-phosphate dehydrogenase (coded by genes GPD1 and GPD2). Glycerol 0-8 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 145-149 26846624-5 2016 Here, we demonstrate that RNA-binding protein Pub1p regulates glycerol production by controlling Gpd1p activity. Glycerol 62-70 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 97-102 27064588-3 2016 In this work we have investigated the expression of four genes (GPD1, GPD2, STL1, and FPS1) crucial in the glycerol pool balance in the four species with a biotechnological potential (S. cerevisiae; S. paradoxus; S. uvarum; and S. kudriavzevii), and the ability of strains to grow under osmotic and cold stresses. Glycerol 107-115 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 64-68 26441028-2 2015 Glycerol, a key precursor for 3-HP biosynthesis was produced by overexpression of the GPD1 and GPP2 genes from Saccharomyces cerevisiae. Glycerol 0-8 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 86-90 26596574-7 2016 In addition, ethanol production was reduced by deleting PDC1 and ADH1 genes encoding major pyruvate decarboxylase and alcohol dehydrogenase, respectively, and glycerol production was eliminated by deleting GPD1 and GPD2 genes encoding glycerol-3-phosphate dehydrogenase. Glycerol 159-167 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 206-210 26338116-2 2015 We have implemented an RT-qPCR (Reverse Transcription-quantitative PCR) methodology with a preventive evaluation of candidate reference genes, to study six target genes related to glycerol synthesis (GPD1, GPD2, GPP2 and GPP1) and flux (STL1 and FPS1), and three ALD genes coding for aldehyde dehydrogenase involved in redox equilibrium via acetate production. Glycerol 180-188 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 200-204 24150518-4 2014 GPD1 silencing gave 20 % less glycerol-3-phosphate dehydrogenase activity, 19 % lower glycerol production, and 9.7 % higher ethanol production compared with the original strain. Glycerol 30-38 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 0-4 26276932-5 2015 The glycerol-producing PTS2 protein glycerol-3-phosphate dehydrogenase Gpd1p shows a tripartite localization in peroxisomes, in the cytosol, and in the nucleus under osmotic stress conditions. Glycerol 4-12 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 71-76 24853351-5 2014 By inactivating ADH1 and ADH4 for ethanol formation and GPD1 and GPD2 for glycerol production, the glycolytic flux was redirected towards acetyl-CoA, resulting in 4-fold improvement in n-butanol production. Glycerol 74-82 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 56-60 24732094-5 2014 This approach revealed that osmotic up-regulation of only two Hog1-dependent glycerol biosynthesis genes, GPD1 and GPP2, is sufficient for successful osmoadaptation. Glycerol 77-85 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 106-110 26604336-7 2015 Meanwhile the yeast overexpressing GPD1 (encoding glycerol-3-phosphate dehydrogenase) produced up to 6 folds more glycerol for beer and wine. Glycerol 50-58 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 35-39 25745106-5 2015 Loss of function of the vacuolar H(+)-ATPase (vma1) or a defect in the biosynthesis of the osmolyte glycerol (gpd1) caused a prolonged repression of housekeeping genes and a delay in gene activation at inducible loci. Glycerol 100-108 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 110-114 25022582-6 2014 CQ-activated Hog1p is translocated to the nucleus and facilitates the expression of GPD1 (glycerol-3-phosphate dehydrogenase), which is required for the synthesis of glycerol (one of the major osmolytes). Glycerol 90-98 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 84-88 23503313-2 2013 A new strategy has been used in the present study for reduced glycerol formation and improved ethanol fermentation performance by finely modulating the expression of GPD1 in the KAM15 strain (fps1Delta pPGK1-GLT1 gpd2Delta). Glycerol 62-70 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 166-170 23537043-2 2013 Here, strains engineered in the promoter of GPD1 and deleted in GPD2 were used to investigate the possibility of reducing glycerol production of Saccharomyces cerevisiae without jeopardising its ability to cope with process stress during ethanol production. Glycerol 122-130 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 44-48 32480969-5 2013 The GPD1 gene was used to overexpress G3PDH, a cytosolic NAD+-dependent key enzyme of cellular glycerol biosynthesis essential for growth of cells under abiotic stresses. Glycerol 95-103 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 4-8 32480969-8 2013 The results also indicate that the gradual increase in glycerol levels in T2 GPD1-transgenic, and gli1 and act1 mutant plants as NaCl level increases whereas they dropped at 200mM NaCl. Glycerol 55-63 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 77-81 21720823-3 2012 Here, we investigated the production of primary and secondary metabolites in S. cerevisiae strains where the glycerol production pathway was inactivated through deletion of the two glycerol-3-phosphate dehydrogenases genes (GPD1/GPD2) and replaced with alternative NAD(+)-generating pathways. Glycerol 109-117 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 224-228 22729542-9 2012 Enhancing glycerol production by increasing expression of the glyceraldehyde-3-phosphate dehydrogenase gene, GPD1, was the most efficient strategy to lower ethanol concentration. Glycerol 10-18 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 109-113 23762021-4 2013 The Hog1 kinase stimulates transcription of genes encoding enzymes required for glycerol production (Gpd1, Gpp2) and glycerol import (Stl1) and activates a regulatory enzyme in glycolysis (Pfk26/27). Glycerol 80-88 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 101-105 22988299-7 2012 Inactivation of Ypk1 by hyperosmotic shock results in dephosphorylation and activation of Gpd1, accelerating recovery through increased glycerol production. Glycerol 136-144 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 90-94 21909679-4 2012 Even though sole deletion of GPD1 or GDH1 reduced glycerol production, double deletion of GPD1 and GDH1 resulted in the lowest glycerol concentration of 2.31 g/L, which was 46.4% lower than the wild-type strain. Glycerol 50-58 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 29-33 21909679-4 2012 Even though sole deletion of GPD1 or GDH1 reduced glycerol production, double deletion of GPD1 and GDH1 resulted in the lowest glycerol concentration of 2.31 g/L, which was 46.4% lower than the wild-type strain. Glycerol 127-135 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 90-94 21126600-5 2011 Although the resultant strain AG1A (gpd1 P(PGK)-gapN) exhibited a 48.7+-0.3% (relative to the amount of substrate consumed) lower glycerol yield and a 7.6+-0.1% (relative to the amount of substrate consumed) higher ethanol yield compared to the wild-type strain, it was sensitive to osmotic stress and failed to ferment on 25% glucose. Glycerol 131-139 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 36-40 21724879-0 2011 Gpd1 and Gpd2 fine-tuning for sustainable reduction of glycerol formation in Saccharomyces cerevisiae. Glycerol 55-63 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 0-4 21724879-1 2011 Gpd1 and Gpd2 are the two isoforms of glycerol 3-phosphate dehydrogenase (GPDH), which is the rate-controlling enzyme of glycerol formation in Saccharomyces cerevisiae. Glycerol 38-46 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 0-4 21724879-4 2011 While single deletions of GPD1 or GPD2 reduced glycerol formation only slightly, the gpd1Delta gpd2Delta double deletion strain produced zero glycerol but showed an osmosensitive phenotype and abolished anaerobic growth. Glycerol 47-55 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 26-30 21724879-4 2011 While single deletions of GPD1 or GPD2 reduced glycerol formation only slightly, the gpd1Delta gpd2Delta double deletion strain produced zero glycerol but showed an osmosensitive phenotype and abolished anaerobic growth. Glycerol 142-150 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 85-89 21188613-3 2011 Under fermentative conditions yeast reoxidizes excess NADH through glycerol production which involves NADH-dependent glycerol-3-phosphate dehydrogenases (Gpd1p and Gpd2p). Glycerol 67-75 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 154-159 16943074-4 2006 Genes involved in ethanol production (PDC genes and ADH1), in glycerol synthesis (GPD1 and HOR2), and in low-affinity hexose transporters (HXT1 and HXT3) were up-regulated at the beginning of model dough-fermentation. Glycerol 62-70 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 82-86 21969862-16 2011 However, expression of LdAQP1 and LdAQP putative in a yeast gpd1Delta strain, deleted for glycerol production, conferred osmosensitive phenotype indicating water transport activity or aquaporin function. Glycerol 90-98 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 60-64 19727706-8 2010 Glycerol is produced to dispose excess cytosolic reduced nicotinamide adenine dinucleotide (NADH), and the regulating step in the pathway is mediated by glycerol 3-phosphate dehydrogenase (encoded by GPD1 and GPD2 genes). Glycerol 0-8 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 200-204 18351334-5 2008 When yeast cells were exposed to 1 M sorbitol stress, the expression of GPD1 encoding glycerol-3-phosphate dehydrogenase is induced, leading to glycerol accumulation. Glycerol 86-94 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 72-76 17953569-15 2007 SIGNIFICANCE AND IMPACT OF THE STUDY: This work confirms that increased acetic acid and glycerol production during Icewine fermentation follows upregulation of ALD3 and GPD1 respectively, but upregulation of ALD3 alone is not sufficient to increase acetic acid production. Glycerol 88-96 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 169-173 19915031-5 2010 Consistent with earlier studies, deletion of the two genes encoding NAD-dependent glycerol-3-phosphate dehydrogenase (GPD1 and GPD2) led to elimination of glycerol production and an inability to grow anaerobically. Glycerol 82-90 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 118-122 19657148-2 2009 The HOG pathway thereby activates the transcription of Gpd1p, an enzyme necessary to synthesize glycerol. Glycerol 96-104 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 55-60 19329666-1 2009 Engineered Saccharomyces cerevisiae strains overexpressing GPD1, which codes for glycerol-3-phosphate dehydrogenase, and lacking the acetaldehyde dehydrogenase Ald6 display large-scale diversion of the carbon flux from ethanol toward glycerol without accumulating acetate. Glycerol 81-89 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 59-63 19424415-4 2009 Deletion of the glycerol biosynthesis genes GPD1, GPD2, or RHR2, among the most up-regulated in long-lived sch9Delta, tor1Delta, and ras2Delta mutants, was sufficient to reverse chronological life span extension in sch9Delta mutants, suggesting that glycerol production, in addition to the regulation of stress resistance systems, optimizes life span extension. Glycerol 16-24 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 44-48 19424415-4 2009 Deletion of the glycerol biosynthesis genes GPD1, GPD2, or RHR2, among the most up-regulated in long-lived sch9Delta, tor1Delta, and ras2Delta mutants, was sufficient to reverse chronological life span extension in sch9Delta mutants, suggesting that glycerol production, in addition to the regulation of stress resistance systems, optimizes life span extension. Glycerol 250-258 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 44-48 17113805-1 2007 The Saccharomyces cerevisiae glycerol pathway (GPD1 and GPP2) was evolved in vivo in Escherichia coli. Glycerol 29-37 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 47-51 17021874-2 2007 In the mutant, KAM-4, the GPD1 gene, which encodes a glycerol 3-phosphate dehydrogenase of S. cerevisiae to synthesize glycerol, was deleted. Glycerol 53-61 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 26-30 15611083-2 2005 In this study we have employed the inability of the Saccharomyces cerevisiae gpd1Delta gpd2Delta mutant both to produce glycerol and to adapt to high osmolarity to study solute transport through aquaglyceroporins and the control of osmostress-induced signaling. Glycerol 120-128 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 77-81 16371351-12 2006 On the contrary, deletion of HOG1 or GPD1 decreased tolerance to freezing of wild-type cells preincubated at a low temperature, whereas no differences could be detected in cells shifted directly from 30 to -20 degrees C. Thus, exposure to low temperatures triggered a Hog1p-dependent accumulation of glycerol, which is essential for freeze protection. Glycerol 300-308 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 37-41 15611083-3 2005 High levels of different polyols, including glycerol, inhibited growth of the gpd1Delta gpd2Delta mutant. Glycerol 44-52 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 78-82 15611083-10 2005 Indeed, when aquaglyceroporin-expressing gpd1Delta gpd2Delta mutants were treated with glycerol, xylitol, or sorbitol, the osmosensing HOG pathway was activated, and the period of activation correlated with the apparent rate of polyol uptake. Glycerol 87-95 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 41-45 15659184-6 2005 CONCLUSIONS: Important levels of expression of GPD1 (but not of other stress genes) are required during the first hours of vinification, because of the need for glycerol production to counteract the hyperosmotic stress at this point. Glycerol 161-169 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 47-51 15960671-8 2005 Expression of GPD1 was upregulated in high sugar fermentations and reflects the elevated levels of glycerol. Glycerol 99-107 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 14-18 15210723-0 2004 Distinct intracellular localization of Gpd1p and Gpd2p, the two yeast isoforms of NAD+-dependent glycerol-3-phosphate dehydrogenase, explains their different contributions to redox-driven glycerol production. Glycerol 97-105 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 39-44 15040952-4 2004 Here, we show that in Saccharomyces cerevisiae, MG exposure increased the internal MG content and activated the expression of GLO1 and GRE3, two genes involved in MG detoxification; GPD1, the gene for glycerol synthesis; and TPS1 and TPS2, the trehalose pathway genes. Glycerol 201-209 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 182-186 15278288-8 2004 Surprisingly, the gpd1gpd2 mutant was found to produce significant amounts of glycerol. Glycerol 78-86 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 18-26 15146068-9 2004 Furthermore, growth improvement by the SFKs on high NaCl plus FK506 was shown to require GPD1, which encodes an NADH-dependent glycerol-3-phosphate dehydrogenase that is important for the production of glycerol in response to osmotic stress. Glycerol 127-135 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 89-93 14734031-4 2004 These results suggest that Zrgpd1p is the primary enzyme involved in Z. rouxii glycerol production, following a mechanism similar to that of S. cerevisiae (Gpd1p). Glycerol 79-87 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 156-161 12702310-3 2002 The amount of glycerol produced by the GPD1-overexpressing yeast in fermentation experiments simulating brewing conditions was increased 5.6 times and ethanol was decreased by 18% when compared to the wild-type. Glycerol 14-22 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 39-43 14618562-0 2003 Glycerol formation during wine fermentation is mainly linked to Gpd1p and is only partially controlled by the HOG pathway. Glycerol 0-8 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 64-69 14618562-1 2003 Glycerol 3-phosphate dehydrogenase, a key enzyme in the production of glycerol, is encoded by GPD1 and GPD2. Glycerol 70-78 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 94-98 12702242-1 2003 Under anaerobic conditions, Saccharomyces cerevisiae uses NADH-dependent glycerol-3-phosphate dehydrogenase (Gpd1p and Gpd2p) to re-oxidize excess NADH, yielding substantial amounts of glycerol. Glycerol 73-81 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 109-114 12073033-8 2002 The plc1Delta hog1Delta strain displays increased osmosensitivity, and has a synthetic defect in glycerol synthesis and the expression of GPD1 (which encodes the enzyme glycerol 3-phosphate dehydrogenase that is involved in glycerol biosynthesis), suggesting that Plc1p and Hog1p function in independent pathways. Glycerol 169-177 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 138-142 14618562-9 2003 These data show that Gpd1p plays a major role in glycerol formation, particularly during the first few hours of exposure to high sugar concentration, and that GPD2 is only of little significance in anaerobic fermentation by wine yeast. Glycerol 49-57 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 21-26 11113971-7 2000 At low demand for NADH reoxidation via glycerol formation, the GPD1, GPD2, GPP1, and GPP2 genes were all expressed at basal levels. Glycerol 39-47 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 63-67 11676566-2 2001 It was recently shown that the most direct approach for increasing glycerol formation is to overexpress GPD1, which encodes the glycerol-3-phosphate dehydrogenase (GPDH) isoform Gpd1p. Glycerol 67-75 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 104-108 11676566-2 2001 It was recently shown that the most direct approach for increasing glycerol formation is to overexpress GPD1, which encodes the glycerol-3-phosphate dehydrogenase (GPDH) isoform Gpd1p. Glycerol 67-75 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 178-183 11676566-12 2001 Overexpression of GPD1 in this strain increased the amount of glycerol produced but led to a pronounced growth defect. Glycerol 62-70 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 18-22 11113971-8 2000 The dynamics of the gene induction and the glycerol formation at low demand for NADH reoxidation point to an important role of the Gpd1p; deletion of the GPD1 gene strongly altered the expression patterns of the GPD2 and GPP1 genes under such conditions. Glycerol 43-51 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 131-136 11113971-8 2000 The dynamics of the gene induction and the glycerol formation at low demand for NADH reoxidation point to an important role of the Gpd1p; deletion of the GPD1 gene strongly altered the expression patterns of the GPD2 and GPP1 genes under such conditions. Glycerol 43-51 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 154-158 10482659-4 1999 Sorbitol and mannitol provided some protection, but less than that generated by a similar concentration of glycerol generated by glycerol-3-P dehydrogenase (GPD1). Glycerol 107-115 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 157-161 10842169-2 2000 The GPD1 gene encodes NAD(+)-dependent glycerol-3-phosphate dehydrogenase, a key enzyme in the production of the compatible solute glycerol. Glycerol 39-47 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 4-8 10931288-2 2000 Consistently, mutant strains gpd1gpd2 and gpp1gpp2, which are devoid of the main glycerol biosynthesis pathway, have been shown to be osmosensitive. Glycerol 81-89 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 29-37 10705374-7 2000 The modest effect of the GPD1 deletion under anaerobic conditions on the maximum specific growth rate and product yields clearly showed that Gdh2p is the important factor in glycerol formation during anaerobic growth. Glycerol 174-182 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 25-29 10705374-10 2000 Deletion of either GPD1 or GPD2 in the wild-type resulted in a dramatic reduction of the glycerol yields in the aerobic batch cultivations of strains TN4 (gpd1-Delta1) and TN5 (gpd2-Delta1) without serious effects on the maximum specific growth rates or the biomass yields. Glycerol 89-97 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 19-23 10641035-4 2000 Proteins with increased expression during growth under sodium chloride stress could be grouped into three classes with respect to PKA activity, with the glycerol metabolic proteins GPD1, GPP2 and DAK1 standing out as independent of PKA. Glycerol 153-161 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 181-185 11042259-3 2000 The rescued mutants display a partially re-established transcriptional control of the osmostress-induced expression of GPD1, a target gene of the HOG pathway encoding NAD(+)-dependent glycerol 3-phosphate dehydrogenase, and a partially recovered hyperosmolarity-induced production of glycerol. Glycerol 184-192 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 119-123 11068916-6 2000 The strains that produced significant glycerol exhibited efficient expression of the glycerol-3-phosphate dehydrogenase gene GPD1. Glycerol 38-46 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 125-129 16232830-0 2000 Enhanced glycerol production in Shochu yeast by heat-shock treatment is due to prolonged transcription of GPD1. Glycerol 9-17 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 106-110 10567692-2 1999 This work examines the role and regulation of GPD1 and GPD2, encoding two isoforms of glycerol 3-phosphate dehydrogenase, in glycerol production during iron starvation. Glycerol 86-94 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 46-50 10567692-5 1999 Deletion of either GPD1 or GPD2 alters the capacity for glycerol production during iron-limited as well as iron sufficient conditions. Glycerol 56-64 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 19-23 10409737-5 1999 hot1 single mutants are specifically compromised in the transient induction of GPD1 and GPP2, which encode enzymes involved in glycerol biosynthesis, and exhibit delayed glycerol accumulation after stress exposure. Glycerol 127-135 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 79-83 12555570-1 1999 The response of the yeast Saccharomyces cerevisiae to osmotic stress is to synthesis and accumulate the glycerol in order to increase the internal osmolarity and this response is controlled by the high-osmolarity glycerol (HOG) response pathway, whose important target gene is GPD1. Glycerol 104-112 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 277-281 12555570-1 1999 The response of the yeast Saccharomyces cerevisiae to osmotic stress is to synthesis and accumulate the glycerol in order to increase the internal osmolarity and this response is controlled by the high-osmolarity glycerol (HOG) response pathway, whose important target gene is GPD1. Glycerol 213-221 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 277-281 12555570-2 1999 The increase of the activity of glycerol-3-phosphate dehydrogenase by over-expression of GPD1 gene can increase the glycerol yield greatly. Glycerol 32-40 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 89-93 9042961-5 1997 Northern analysis of the salt induced transcription of GPD1 and GPP2, encoding the cytosolic glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase involved in the osmostress induced glycerol production, demonstrated an about 20-fold PBS2-dependent transient activation, in agreement with the previously reported transient nature of the signal transduced by the HOG pathway. Glycerol 93-101 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 55-59 9830094-3 1998 Also the gpd1 delta mutant showed a slight decrease in glycerol formation but to a much lesser degree. Glycerol 55-63 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 9-13 9559543-10 1998 Our results also demonstrated that of the two isoforms of NAD-dependent glycerol 3-phosphate dehydrogenase, only the enzyme encoded by GPD1 appeared important for the shuttle, since the enhanced glycerol production that occurs in a gut2 delta strain proved dependent on GPD1 but not on GPD2. Glycerol 72-80 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 135-139 9559543-10 1998 Our results also demonstrated that of the two isoforms of NAD-dependent glycerol 3-phosphate dehydrogenase, only the enzyme encoded by GPD1 appeared important for the shuttle, since the enhanced glycerol production that occurs in a gut2 delta strain proved dependent on GPD1 but not on GPD2. Glycerol 72-80 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 270-274 9446611-10 1998 Glycerol is synthesized from glucose, and a rate-limiting enzyme in glycerol biosynthesis is glycerol-3-phosphate dehydrogenase (GPD1 gene product), which catalyzes reduction of dihydroxyacetone phosphate to glycerol 3-phosphate. Glycerol 0-8 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 129-133 9446611-10 1998 Glycerol is synthesized from glucose, and a rate-limiting enzyme in glycerol biosynthesis is glycerol-3-phosphate dehydrogenase (GPD1 gene product), which catalyzes reduction of dihydroxyacetone phosphate to glycerol 3-phosphate. Glycerol 68-76 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 129-133 9298657-4 1997 One of the presumed key-enzymes in the production of glycerol in the cell is glycerol 3-phosphate dehydrogenase encoded by the GPD1 gene. Glycerol 53-61 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 127-131 9234667-7 1997 Overexpression of GPD1 on synthetic must (200 g/l glucose) resulted in a substantial increase in glycerol production ( x 4) at the expense of ethanol. Glycerol 97-105 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 18-22 9872772-1 1999 Overexpression of the GPD1 gene, encoding a glycerol-3-phosphate dehydrogenase, resulted in a 1.5- to 2.5-fold increase in glycerol production and a slight decrease in ethanol formation under conditions simulating wine fermentation. Glycerol 124-132 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 23-27 9872772-4 1999 Wine strains overproducing glycerol at moderate levels (12 to 18 g/liter) reduced acetoin almost completely to 2,3-butanediol. A lower biomass concentration was attained by GPD1-overexpressing strains, probably due to high acetaldehyde production during the growth phase. Glycerol 28-36 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 174-178 8923738-3 1996 The glycerol yield was 4.7 times (a pdc mutant exhibiting 19% of normal PDC activity) and 6.5 times (a strain exhibiting 20-fold increased GPD activity resulting from overexpression of GPD1 gene) that of the wild type. Glycerol 4-12 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 185-189 8923738-6 1996 The rate of glycerol formation in the pdc mutant was, due to a slower rate of glucose catabolism, only twice that of the wild type, and was increased by GPD1 overexpression to three times that of the wild-type level. Glycerol 12-20 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 153-157 8923738-7 1996 Overexpression of GPD1 in the wild-type background, however, led to a six- to seven-fold increase in the rate of glycerol formation. Glycerol 113-121 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 18-22 7729414-3 1995 Enhanced glycerol production caused by overexpression of GPD1 encoding glycerol-3-phosphate dehydrogenase also suppressed the growth defect of ggs1/tps1 delta mutants, suggesting a novel role for glycerol production in the control of glycolysis. Glycerol 9-17 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 57-61 7729414-3 1995 Enhanced glycerol production caused by overexpression of GPD1 encoding glycerol-3-phosphate dehydrogenase also suppressed the growth defect of ggs1/tps1 delta mutants, suggesting a novel role for glycerol production in the control of glycolysis. Glycerol 71-79 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 57-61 7500933-5 1995 Gpd1p is a key enzyme in the synthesis of glycerol, which is a major osmoprotectant in S. cerevisiae. Glycerol 42-50 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 0-5 7500933-6 1995 Cloning of HOR1/GPD1 as a HOR gene indicates that the accumulation of glycerol in yeast cells under hyperosmotic stress is, at least in part, caused by an increase in the level of GPDH protein. Glycerol 70-78 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 11-15 7500933-6 1995 Cloning of HOR1/GPD1 as a HOR gene indicates that the accumulation of glycerol in yeast cells under hyperosmotic stress is, at least in part, caused by an increase in the level of GPDH protein. Glycerol 70-78 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 16-20 7961476-5 1994 Disruption of DAR1 in a haploid S. cerevisiae was not lethal but led to a decrease in cytoplasmic NADH-dependent G3PDase activity, an increase in osmotic sensitivity, and a 25% reduction in glycerol secretion from cells grown anaerobically on glucose. Glycerol 190-198 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 14-18 8196651-0 1994 GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway. Glycerol 20-28 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 0-4 8196651-2 1994 We have cloned a gene encoding the key enzyme of glycerol synthesis, the NADH-dependent cytosolic glycerol-3-phosphate dehydrogenase, and we named it GPD1. Glycerol 49-57 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 150-154 8196651-3 1994 gpd1 delta mutants produced very little glycerol, and they were sensitive to osmotic stress. Glycerol 40-48 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 0-4 7934860-4 1993 The osg1-1 mutant, described in this work, is unable to grow at low water potential and shows a decreased capacity for glycerol production and a strongly reduced activity of NAD(+)-dependent sn-glycerol 3-phosphate dehydrogenase (GPD), an enzyme in the glycerol-producing pathway. Glycerol 119-127 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 4-10 7934860-4 1993 The osg1-1 mutant, described in this work, is unable to grow at low water potential and shows a decreased capacity for glycerol production and a strongly reduced activity of NAD(+)-dependent sn-glycerol 3-phosphate dehydrogenase (GPD), an enzyme in the glycerol-producing pathway. Glycerol 194-202 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 4-10 34369003-1 2021 Glycerol 3-phosphate dehydrogenase (Gpd1 isoform) catalyzes the rate limiting step of glycerol synthesis and is a critical component of the osmo-responsive machinery in yeast. Glycerol 86-94 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 36-40 35352941-6 2022 Thus, the error-prone DNA repair protein was employed to develop a novel mutagenic genome editing (mGE) strategy, which can increase the mutation numbers and effectively improve the ethanol/glycerol ratio of Saccharomyces cerevisiae through modulating the expression of FPS1 and GPD1. Glycerol 190-198 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 Saccharomyces cerevisiae S288C 279-283