PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 16047153-4 2005 Examined in the presence of 1 microM TTX and 5 mM TEA with 10 mM Hepes in the recording pipette, NH(3) and TMeA increased pH(i) and the magnitudes of depolarization-evoked intracellular [Ca(2+)] transients, Ca(2+)-dependent depolarizing potentials, and inward Ca(2+) currents but reduced the slow AHP and sI(ahp). HEPES 65-70 glucose-6-phosphate isomerase Rattus norvegicus 122-127 21887217-5 2011 In the HEPES buffered Krebs-Henseleit (KH) solution, the intracellular pH (pHi) recovery from NH(4)Cl induced acidification in the cultured caput epididymal epithelium was completely inhibited by amiloride, the inhibitor of Na(+)/H(+) exchanger (NHE). HEPES 7-12 glucose-6-phosphate isomerase Rattus norvegicus 71-73 21887217-5 2011 In the HEPES buffered Krebs-Henseleit (KH) solution, the intracellular pH (pHi) recovery from NH(4)Cl induced acidification in the cultured caput epididymal epithelium was completely inhibited by amiloride, the inhibitor of Na(+)/H(+) exchanger (NHE). HEPES 7-12 glucose-6-phosphate isomerase Rattus norvegicus 75-78 21887217-6 2011 Immediately changing of the KH solution from HEPES buffered to HCO(3)(-) buffered would cause another pHi recovery. HEPES 45-50 glucose-6-phosphate isomerase Rattus norvegicus 102-105 9746485-5 1998 These changes were reversed by returning cells to the control pHi of 7.0 and were eliminated by dialyzing cells with pipette solution containing 50 mmol/l HEPES to buffer NH4Cl-induced changes in pHi. HEPES 155-160 glucose-6-phosphate isomerase Rattus norvegicus 196-199 10199608-7 1999 Reducing pHi at a constant pHo (by exposure to pH 7.3 HCO3-/CO2-free medium buffered with 30 mM HEPES) also attenuated fast and slow afterhyperpolarizations. HEPES 96-101 glucose-6-phosphate isomerase Rattus norvegicus 9-12 10199608-7 1999 Reducing pHi at a constant pHo (by exposure to pH 7.3 HCO3-/CO2-free medium buffered with 30 mM HEPES) also attenuated fast and slow afterhyperpolarizations. HEPES 96-101 glucose-6-phosphate isomerase Rattus norvegicus 9-11 10707899-3 1999 In nominally bicarbonate-free (Hepes-buffered) medium, a marked pHi recovery from internal acid load was seen which could be blocked completely by 30 microM HOE 694, a specific Na+-H+ exchanger isoform 1(NHE-1) inhibitor, at a pHi above 6.9. HEPES 31-36 glucose-6-phosphate isomerase Rattus norvegicus 64-67 9679168-5 1998 At room temperature, the ratio DeltapHi : DeltapHo (the slope of the regression line relating pHi to pHo) was 0.37 under HCO3-/CO2-buffered conditions and 0.45 under Hepes-buffered conditions; corresponding values at 37 C were 0.71 and 0.79, respectively. HEPES 166-171 glucose-6-phosphate isomerase Rattus norvegicus 36-39 9592070-3 1998 Replacement of CO2/HCO3- in the bath by HEPES (26 mM, pH 7.4) for 10 min acidified pHi (0.07 +/- 0.03 units) and also excited VLN(CS). HEPES 40-45 glucose-6-phosphate isomerase Rattus norvegicus 83-86 9644216-3 1998 We observed a pHi of 6.88+/-0.012 (n=24) in resting mast cells exposed to a HEPES buffer (pH 7.4), but a sustained drop of 0.21 pH units to 6.67+/-0.015 (n=23) when we exposed the mast cells to a HEPES/HCO3- buffer equilibrated at all time with 5% CO2 (pH 7.4). HEPES 76-81 glucose-6-phosphate isomerase Rattus norvegicus 14-17 9644216-9 1998 Histamine release stimulated by antigen and compound 48/80 was substantially reduced in the presence of HEPES/ HCO3- buffer (pHo 7.4, pHi 6.66). HEPES 104-109 glucose-6-phosphate isomerase Rattus norvegicus 134-137 9114240-8 1997 The GABA-induced pHi decrease, but not the accompanying I(m) and g(m) responses, was suppressed in CO2/HCO3(-)-free, N-2-hydroxy-ethylpiperazine-N"-2-ethane sulphonic acid pH-buffered solution. HEPES 117-171 glucose-6-phosphate isomerase Rattus norvegicus 17-20 9366082-3 1997 In another type of experiment, changes in extracellular pH of solutions containing HEPES or HCO3-/CO2 buffers led to significant changes in pHi that did not seem to be back-regulated efficiently by duct cells. HEPES 83-88 glucose-6-phosphate isomerase Rattus norvegicus 140-143 9316422-2 1997 In N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid-buffered solutions, TRC pHi responded rapidly and monotonically to changes in pHo between 6.5 and 8.0. HEPES 3-54 glucose-6-phosphate isomerase Rattus norvegicus 79-82 8930837-12 1996 The GABA-induced pHi decrease, but not the conductance increase, was suppressed in Hepes solution. HEPES 83-88 glucose-6-phosphate isomerase Rattus norvegicus 17-20 8841993-8 1996 When neurons with a relatively low initial pHi in Hepes-buffered solutions were acid loaded, pHi recovered very slowly. HEPES 50-55 glucose-6-phosphate isomerase Rattus norvegicus 43-46 8841993-8 1996 When neurons with a relatively low initial pHi in Hepes-buffered solutions were acid loaded, pHi recovered very slowly. HEPES 50-55 glucose-6-phosphate isomerase Rattus norvegicus 93-96 8841993-23 1996 Neurons with a relatively high pHi in Hepes buffer continued to be more alkaline (by approximately 0.2 pH units) in CO2/HCO3-. HEPES 38-43 glucose-6-phosphate isomerase Rattus norvegicus 31-34 8841993-25 1996 When neurons with a relatively high initial pHi in Hepes (> or = 7.25) were exposed to CO2/HCO3- and then acid loaded, Jtotal values were more than twice the highest values observed in neurons with lower initial pHi values. HEPES 51-56 glucose-6-phosphate isomerase Rattus norvegicus 44-47 8841993-26 1996 Neurons with a moderate initial pHi in Hepes (7.05-7.24) had Jtotal values, at comparable pHi values, that were approximately 2-fold greater than for neurons with a relatively low initial pHi (< 7.05). HEPES 39-44 glucose-6-phosphate isomerase Rattus norvegicus 32-35 8841993-29 1996 Those with higher initial pHi values in a Hepes buffer tend to have greater Jtotal values in both Hepes and CO2/HCO3-, and tend to have higher steady-state pHi values in CO2/HCO3-. HEPES 42-47 glucose-6-phosphate isomerase Rattus norvegicus 26-29 8841993-29 1996 Those with higher initial pHi values in a Hepes buffer tend to have greater Jtotal values in both Hepes and CO2/HCO3-, and tend to have higher steady-state pHi values in CO2/HCO3-. HEPES 98-103 glucose-6-phosphate isomerase Rattus norvegicus 26-29 7590668-3 1995 Steady-state intracellular pH (pHi) in a bicarbonate-free solution (HEPES) were 7.17 +/- 0.031 in PP and 7.15 +/- 0.041 in PV cells. HEPES 68-73 glucose-6-phosphate isomerase Rattus norvegicus 31-34 8851498-5 1996 Following an intracellular acid load (induced by 10 mM NH4Cl removal), pHi recovery in HEPES-buffered Tyrode solution was significantly slowed down upon application of 0.3 mM TMZ only when myocytes were pretreated for 5 h 30 min (slowing by approximately 50%; P < 0.01). HEPES 87-92 glucose-6-phosphate isomerase Rattus norvegicus 71-74 7588286-3 1995 The resting intracellular pH (pHi) was approximately 7.15 in astrocytes exposed to CO2/HCO3(-)-free medium buffered with HEPES at pH 7.40 at 22 C. Isoosmotic replacement of extracellular sodium by mannitol or choline decreased the pHi by 0.15 pH unit and reduced uptake by about 20%. HEPES 121-126 glucose-6-phosphate isomerase Rattus norvegicus 30-33 8747226-8 1995 On changing from oxygenated bicarbonate ACSF to either 10 or 25 mM HEPES ACSF, pHi decreased by 0.13-0.15 units, and the membrane depolarized by 10-11 mV. HEPES 67-72 glucose-6-phosphate isomerase Rattus norvegicus 79-82 8747226-12 1995 In bicarbonate ACSF and in 25 mM HEPES ACSF, there was a significant linear relationship between prehypoxic pHi and the direction and amplitude of the hypoxia-induced membrane potential change (either an hyperpolarization or a depolarization). HEPES 33-38 glucose-6-phosphate isomerase Rattus norvegicus 108-111 7590668-7 1995 Culture with TGF beta 1 for 7 hours induced (in HEPES) a decrease of pHi recovery rate from an acid load more in PV (by 46%) than in PP hepatocytes (by 35%, P < .05). HEPES 48-53 glucose-6-phosphate isomerase Rattus norvegicus 69-72 7558242-2 1995 Application of glutamate or kainate (100 microM) in a HEPES-buffered, CO2/HCO3(-) -free saline induced a decrease in pHi and an increase in Ca2+i. HEPES 54-59 glucose-6-phosphate isomerase Rattus norvegicus 117-120 7865219-6 1995 Substitution of gluconate for Cl- in the basolateral fluid, but not the apical fluid, resulted in a rise in steady-state pHi that was reversible on replacement of the basolateral fluid with Cl(-)-containing buffer, which occurred in HCO3(-)- but not Hepes-buffered medium. HEPES 250-255 glucose-6-phosphate isomerase Rattus norvegicus 121-124 7708483-2 1994 The steady-state pHi was 6.96 in both nominally CO2/HCO3(-)-free, HEPES-buffered saline (6.96 +/- 0.14; n = 48) and in a saline containing 5% CO2/24 mM HCO3- (6.96 +/- 0.18; n = 48) (at pH 7.4). HEPES 66-71 glucose-6-phosphate isomerase Rattus norvegicus 17-20 7923632-2 1994 In modified Krebs" solution containing 20 mmol/L HEPES and 4.4 mmol/L HCO3-, isoproterenol (1 mumol/L) caused a significant decrease of steady-state pHi from 7.20 +/- 0.02 to 7.13 +/- 0.02 (mean +/- SEM) within 2 minutes. HEPES 49-54 glucose-6-phosphate isomerase Rattus norvegicus 149-152 8397224-3 1993 Basal pHi of BDE was 7.04 +/- 0.06 in Hepes and 7.16 +/- 0.10 in KRB and was unaffected by secretin (50-200 nM). HEPES 38-43 glucose-6-phosphate isomerase Rattus norvegicus 6-9 8048524-4 1994 In HEPES-buffered solutions, basal pHi was 7.31 +/- 0.01 (n = 68), and pHi was dependent on extracellular Na+. HEPES 3-8 glucose-6-phosphate isomerase Rattus norvegicus 35-38 8048524-4 1994 In HEPES-buffered solutions, basal pHi was 7.31 +/- 0.01 (n = 68), and pHi was dependent on extracellular Na+. HEPES 3-8 glucose-6-phosphate isomerase Rattus norvegicus 71-74 8384799-9 1993 A similar reduction in pHi in a CO2-HCO3(-)-free, N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid-buffered Ringer also did not stimulate Na+ absorption. HEPES 50-101 glucose-6-phosphate isomerase Rattus norvegicus 23-26 8406681-2 1993 When exposed to a nominally CO2/HCO3(-)-free medium buffered to pH 7.40 with HEPES at 37 degrees C, the cells had a mean pHi of 6.89. HEPES 77-82 glucose-6-phosphate isomerase Rattus norvegicus 121-124 8406681-5 1993 In experiments in which astrocytes were exposed to nominally HCO3(-)-free (HEPES-buffered) solutions, the application and withdrawal of 20 mM extracellular NH4+ caused pHi to fall to a value substantially below the initial one. HEPES 75-80 glucose-6-phosphate isomerase Rattus norvegicus 168-171 8406681-7 1993 In other experiments conducted on cell bathed in HEPES-buffered solutions, removing extracellular Na+ caused pHi to decrease rapidly by 0.5. HEPES 49-54 glucose-6-phosphate isomerase Rattus norvegicus 109-112 1316162-2 1992 Basal intracellular pH (pHi) was 7.13 +/- 0.10 in cells incubated in Hepes-buffered saline solution. HEPES 69-74 glucose-6-phosphate isomerase Rattus norvegicus 24-27 12106376-2 1992 The steady-state pHi was estimated as 7.27 +/- 0.25 in bicarbonate-buffered media and 7.49 +/- 0.35 in HEPES-buffered media. HEPES 103-108 glucose-6-phosphate isomerase Rattus norvegicus 17-20 1317431-9 1992 The alpha 1-adrenoceptor-mediated increase in intracellular pH (pHi) was 0.1 pH unit in HEPES buffer containing 4.4 mM-NaHCO3 and in Krebs buffer containing 25 mM-NaHCO3. HEPES 88-93 glucose-6-phosphate isomerase Rattus norvegicus 64-67 1822550-4 1991 Basal pHi in HEPES-buffered solution (containing 4.4 mM-NaHCO3) was 7.08. HEPES 13-18 glucose-6-phosphate isomerase Rattus norvegicus 6-9 1660595-2 1991 The pHi in the TRIS/HEPES-buffered standard solution was 7.29 +/- 0.01. HEPES 20-25 glucose-6-phosphate isomerase Rattus norvegicus 4-7 1694402-2 1990 In cells superfused with N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid-buffered solution at pH 7.40, basal pHi was determined to be 7.15 +/- 0.13. HEPES 25-76 glucose-6-phosphate isomerase Rattus norvegicus 113-116 2213591-9 1990 In bicarbonate-free (HEPES-buffered) solution the steady-state pHi was 7.37 +/- 0.02 (n = 19), significantly higher than in bicarbonate-buffered solution. HEPES 21-26 glucose-6-phosphate isomerase Rattus norvegicus 63-66 3436883-7 1987 Because PMA, NH4Cl, methylamine, imidazole, HEPES-buffered solutions, and low-Cl- solutions can cause increases in pHi and amiloride and acetate can cause decreases in pHi, these results suggest that intracellular alkalosis and acidosis, respectively, potentiate and blunt vasoconstrictor responses to hypoxia and other stimuli in isolated rat lungs. HEPES 44-49 glucose-6-phosphate isomerase Rattus norvegicus 115-118 2318379-7 1990 It was found that measurements of [Ca2+]i with fura 2 were influenced by shifts in pHi that occur when cells are incubated in either HEPES-buffered or CO2/HCO3- media of differing pHo values. HEPES 133-138 glucose-6-phosphate isomerase Rattus norvegicus 83-86 2318379-8 1990 However, at any given value of pHi, the apparent [Ca2+]i measured in cells incubated in HEPES-buffered media was slightly higher than in cells incubated in CO2/HCO3- buffered media. HEPES 88-93 glucose-6-phosphate isomerase Rattus norvegicus 31-34 2610271-2 1989 Intracellular pH (pHi) was acutely lowered by NH3 prepulse in HCO3(-)-free medium buffered with 6 mM N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid, and its recovery was measured thereafter under control conditions, in the presence of amiloride to inhibit Na(+)-H+ antiport, and in the presence of N-ethylmaleimide (NEM), a plasma membrane H(+)-ATPase inhibitor. HEPES 101-152 glucose-6-phosphate isomerase Rattus norvegicus 18-21 2838077-3 1988 The pHi of cells in physiological saline buffered with Hepes (pH 7.3) at 37 degrees C was close to 7.0. HEPES 55-60 glucose-6-phosphate isomerase Rattus norvegicus 4-7 2153100-3 1990 At the age of 16-20 weeks, pHi of lymphocytes suspended in a HCO3-free HEPES-buffered solution, was markedly lower in the SHR than in the WKY rats (7.07 +/- 0.02, n = 16 and 7.22 +/- 0.01, n = 15, respectively, p less than 0.001), whereas systolic blood pressure was higher in SHR than in WKY rats (175 +/- 5.0 and 105 +/- 3.0 mm Hg, respectively, p less than 0.001). HEPES 71-76 glucose-6-phosphate isomerase Rattus norvegicus 27-30 2551179-3 1989 In N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solutions, pHi was 7.14, and intrinsic buffer capacity was inversely related to pHi. HEPES 3-54 glucose-6-phosphate isomerase Rattus norvegicus 83-86 2551179-3 1989 In N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solutions, pHi was 7.14, and intrinsic buffer capacity was inversely related to pHi. HEPES 3-54 glucose-6-phosphate isomerase Rattus norvegicus 152-155 2551179-3 1989 In N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solutions, pHi was 7.14, and intrinsic buffer capacity was inversely related to pHi. HEPES 56-61 glucose-6-phosphate isomerase Rattus norvegicus 83-86 2551179-3 1989 In N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solutions, pHi was 7.14, and intrinsic buffer capacity was inversely related to pHi. HEPES 56-61 glucose-6-phosphate isomerase Rattus norvegicus 152-155 2522833-3 1989 The pHi of ventricular cells bathed in HEPES-buffered medium averaged 7.30 +/- 0.02. HEPES 39-44 glucose-6-phosphate isomerase Rattus norvegicus 4-7 2614727-5 1989 In a HEPES-buffered bathing solution of pH 7.4, pHi had a mean value of 7.16 +/- 0.05 (mean +/- S.E.M.). HEPES 5-10 glucose-6-phosphate isomerase Rattus norvegicus 40-42 2614727-5 1989 In a HEPES-buffered bathing solution of pH 7.4, pHi had a mean value of 7.16 +/- 0.05 (mean +/- S.E.M.). HEPES 5-10 glucose-6-phosphate isomerase Rattus norvegicus 48-51