PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
16047153-4	2005	Examined in the presence of 1 microM TTX and 5 mM TEA with 10 mM Hepes in the recording pipette, NH(3) and TMeA increased pH(i) and the magnitudes of depolarization-evoked intracellular [Ca(2+)] transients, Ca(2+)-dependent depolarizing potentials, and inward Ca(2+) currents but reduced the slow AHP and sI(ahp).	HEPES	65-70	glucose-6-phosphate isomerase	Rattus norvegicus	122-127	
10199608-7	1999	Reducing pHi at a constant pHo (by exposure to pH 7.3 HCO3-/CO2-free medium buffered with 30 mM HEPES) also attenuated fast and slow afterhyperpolarizations.	HEPES	96-101	glucose-6-phosphate isomerase	Rattus norvegicus	9-12	
10707899-3	1999	In nominally bicarbonate-free (Hepes-buffered) medium, a marked pHi recovery from internal acid load was seen which could be blocked completely by 30 microM HOE 694, a specific Na+-H+ exchanger isoform 1(NHE-1) inhibitor, at a pHi above 6.9.	HEPES	31-36	glucose-6-phosphate isomerase	Rattus norvegicus	64-67	
10199608-7	1999	Reducing pHi at a constant pHo (by exposure to pH 7.3 HCO3-/CO2-free medium buffered with 30 mM HEPES) also attenuated fast and slow afterhyperpolarizations.	HEPES	96-101	glucose-6-phosphate isomerase	Rattus norvegicus	9-11	
9746485-5	1998	These changes were reversed by returning cells to the control pHi of 7.0 and were eliminated by dialyzing cells with pipette solution containing 50 mmol/l HEPES to buffer NH4Cl-induced changes in pHi.	HEPES	155-160	glucose-6-phosphate isomerase	Rattus norvegicus	196-199	
9644216-3	1998	We observed a pHi of 6.88+/-0.012 (n=24) in resting mast cells exposed to a HEPES buffer (pH 7.4), but a sustained drop of 0.21 pH units to 6.67+/-0.015 (n=23) when we exposed the mast cells to a HEPES/HCO3- buffer equilibrated at all time with 5% CO2 (pH 7.4).	HEPES	76-81	glucose-6-phosphate isomerase	Rattus norvegicus	14-17	
9679168-5	1998	At room temperature, the ratio DeltapHi : DeltapHo (the slope of the regression line relating pHi to pHo) was 0.37 under HCO3-/CO2-buffered conditions and 0.45 under Hepes-buffered conditions; corresponding values at 37 C were 0.71 and 0.79, respectively.	HEPES	166-171	glucose-6-phosphate isomerase	Rattus norvegicus	36-39	
9644216-9	1998	Histamine release stimulated by antigen and compound 48/80 was substantially reduced in the presence of HEPES/ HCO3- buffer (pHo 7.4, pHi 6.66).	HEPES	104-109	glucose-6-phosphate isomerase	Rattus norvegicus	134-137	
9592070-3	1998	Replacement of CO2/HCO3- in the bath by HEPES (26 mM, pH 7.4) for 10 min acidified pHi (0.07 +/- 0.03 units) and also excited VLN(CS).	HEPES	40-45	glucose-6-phosphate isomerase	Rattus norvegicus	83-86	
9366082-3	1997	In another type of experiment, changes in extracellular pH of solutions containing HEPES or HCO3-/CO2 buffers led to significant changes in pHi that did not seem to be back-regulated efficiently by duct cells.	HEPES	83-88	glucose-6-phosphate isomerase	Rattus norvegicus	140-143	
8930837-12	1996	The GABA-induced pHi decrease, but not the conductance increase, was suppressed in Hepes solution.	HEPES	83-88	glucose-6-phosphate isomerase	Rattus norvegicus	17-20	
9316422-2	1997	In N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid-buffered solutions, TRC pHi responded rapidly and monotonically to changes in pHo between 6.5 and 8.0.	HEPES	3-54	glucose-6-phosphate isomerase	Rattus norvegicus	79-82	
9114240-8	1997	The GABA-induced pHi decrease, but not the accompanying I(m) and g(m) responses, was suppressed in CO2/HCO3(-)-free, N-2-hydroxy-ethylpiperazine-N"-2-ethane sulphonic acid pH-buffered solution.	HEPES	117-171	glucose-6-phosphate isomerase	Rattus norvegicus	17-20	
7588286-3	1995	The resting intracellular pH (pHi) was approximately 7.15 in astrocytes exposed to CO2/HCO3(-)-free medium buffered with HEPES at pH 7.40 at 22 C. Isoosmotic replacement of extracellular sodium by mannitol or choline decreased the pHi by 0.15 pH unit and reduced uptake by about 20%.	HEPES	121-126	glucose-6-phosphate isomerase	Rattus norvegicus	30-33	
8851498-5	1996	Following an intracellular acid load (induced by 10 mM NH4Cl removal), pHi recovery in HEPES-buffered Tyrode solution was significantly slowed down upon application of 0.3 mM TMZ only when myocytes were pretreated for 5 h 30 min (slowing by approximately 50%; P < 0.01).	HEPES	87-92	glucose-6-phosphate isomerase	Rattus norvegicus	71-74	
8841993-8	1996	When neurons with a relatively low initial pHi in Hepes-buffered solutions were acid loaded, pHi recovered very slowly.	HEPES	50-55	glucose-6-phosphate isomerase	Rattus norvegicus	43-46	
8841993-8	1996	When neurons with a relatively low initial pHi in Hepes-buffered solutions were acid loaded, pHi recovered very slowly.	HEPES	50-55	glucose-6-phosphate isomerase	Rattus norvegicus	93-96	
8841993-23	1996	Neurons with a relatively high pHi in Hepes buffer continued to be more alkaline (by approximately 0.2 pH units) in CO2/HCO3-.	HEPES	38-43	glucose-6-phosphate isomerase	Rattus norvegicus	31-34	
8841993-25	1996	When neurons with a relatively high initial pHi in Hepes (> or = 7.25) were exposed to CO2/HCO3- and then acid loaded, Jtotal values were more than twice the highest values observed in neurons with lower initial pHi values.	HEPES	51-56	glucose-6-phosphate isomerase	Rattus norvegicus	44-47	
8841993-26	1996	Neurons with a moderate initial pHi in Hepes (7.05-7.24) had Jtotal values, at comparable pHi values, that were approximately 2-fold greater than for neurons with a relatively low initial pHi (< 7.05).	HEPES	39-44	glucose-6-phosphate isomerase	Rattus norvegicus	32-35	
8841993-29	1996	Those with higher initial pHi values in a Hepes buffer tend to have greater Jtotal values in both Hepes and CO2/HCO3-, and tend to have higher steady-state pHi values in CO2/HCO3-.	HEPES	42-47	glucose-6-phosphate isomerase	Rattus norvegicus	26-29	
8841993-29	1996	Those with higher initial pHi values in a Hepes buffer tend to have greater Jtotal values in both Hepes and CO2/HCO3-, and tend to have higher steady-state pHi values in CO2/HCO3-.	HEPES	98-103	glucose-6-phosphate isomerase	Rattus norvegicus	26-29	
7590668-3	1995	Steady-state intracellular pH (pHi) in a bicarbonate-free solution (HEPES) were 7.17 +/- 0.031 in PP and 7.15 +/- 0.041 in PV cells.	HEPES	68-73	glucose-6-phosphate isomerase	Rattus norvegicus	31-34	
8747226-8	1995	On changing from oxygenated bicarbonate ACSF to either 10 or 25 mM HEPES ACSF, pHi decreased by 0.13-0.15 units, and the membrane depolarized by 10-11 mV.	HEPES	67-72	glucose-6-phosphate isomerase	Rattus norvegicus	79-82	
8747226-12	1995	In bicarbonate ACSF and in 25 mM HEPES ACSF, there was a significant linear relationship between prehypoxic pHi and the direction and amplitude of the hypoxia-induced membrane potential change (either an hyperpolarization or a depolarization).	HEPES	33-38	glucose-6-phosphate isomerase	Rattus norvegicus	108-111	
7590668-7	1995	Culture with TGF beta 1 for 7 hours induced (in HEPES) a decrease of pHi recovery rate from an acid load more in PV (by 46%) than in PP hepatocytes (by 35%, P < .05).	HEPES	48-53	glucose-6-phosphate isomerase	Rattus norvegicus	69-72	
7558242-2	1995	Application of glutamate or kainate (100 microM) in a HEPES-buffered, CO2/HCO3(-) -free saline induced a decrease in pHi and an increase in Ca2+i.	HEPES	54-59	glucose-6-phosphate isomerase	Rattus norvegicus	117-120	
7923632-2	1994	In modified Krebs" solution containing 20 mmol/L HEPES and 4.4 mmol/L HCO3-, isoproterenol (1 mumol/L) caused a significant decrease of steady-state pHi from 7.20 +/- 0.02 to 7.13 +/- 0.02 (mean +/- SEM) within 2 minutes.	HEPES	49-54	glucose-6-phosphate isomerase	Rattus norvegicus	149-152	
7865219-6	1995	Substitution of gluconate for Cl- in the basolateral fluid, but not the apical fluid, resulted in a rise in steady-state pHi that was reversible on replacement of the basolateral fluid with Cl(-)-containing buffer, which occurred in HCO3(-)- but not Hepes-buffered medium.	HEPES	250-255	glucose-6-phosphate isomerase	Rattus norvegicus	121-124	
12106376-2	1992	The steady-state pHi was estimated as 7.27 +/- 0.25 in bicarbonate-buffered media and 7.49 +/- 0.35 in HEPES-buffered media.	HEPES	103-108	glucose-6-phosphate isomerase	Rattus norvegicus	17-20	
8048524-4	1994	In HEPES-buffered solutions, basal pHi was 7.31 +/- 0.01 (n = 68), and pHi was dependent on extracellular Na+.	HEPES	3-8	glucose-6-phosphate isomerase	Rattus norvegicus	35-38	
8048524-4	1994	In HEPES-buffered solutions, basal pHi was 7.31 +/- 0.01 (n = 68), and pHi was dependent on extracellular Na+.	HEPES	3-8	glucose-6-phosphate isomerase	Rattus norvegicus	71-74	
8397224-3	1993	Basal pHi of BDE was 7.04 +/- 0.06 in Hepes and 7.16 +/- 0.10 in KRB and was unaffected by secretin (50-200 nM).	HEPES	38-43	glucose-6-phosphate isomerase	Rattus norvegicus	6-9	
8384799-9	1993	A similar reduction in pHi in a CO2-HCO3(-)-free, N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid-buffered Ringer also did not stimulate Na+ absorption.	HEPES	50-101	glucose-6-phosphate isomerase	Rattus norvegicus	23-26	
7708483-2	1994	The steady-state pHi was 6.96 in both nominally CO2/HCO3(-)-free, HEPES-buffered saline (6.96 +/- 0.14; n = 48) and in a saline containing 5% CO2/24 mM HCO3- (6.96 +/- 0.18; n = 48) (at pH 7.4).	HEPES	66-71	glucose-6-phosphate isomerase	Rattus norvegicus	17-20	
8406681-2	1993	When exposed to a nominally CO2/HCO3(-)-free medium buffered to pH 7.40 with HEPES at 37 degrees C, the cells had a mean pHi of 6.89.	HEPES	77-82	glucose-6-phosphate isomerase	Rattus norvegicus	121-124	
8406681-5	1993	In experiments in which astrocytes were exposed to nominally HCO3(-)-free (HEPES-buffered) solutions, the application and withdrawal of 20 mM extracellular NH4+ caused pHi to fall to a value substantially below the initial one.	HEPES	75-80	glucose-6-phosphate isomerase	Rattus norvegicus	168-171	
8406681-7	1993	In other experiments conducted on cell bathed in HEPES-buffered solutions, removing extracellular Na+ caused pHi to decrease rapidly by 0.5.	HEPES	49-54	glucose-6-phosphate isomerase	Rattus norvegicus	109-112	
1316162-2	1992	Basal intracellular pH (pHi) was 7.13 +/- 0.10 in cells incubated in Hepes-buffered saline solution.	HEPES	69-74	glucose-6-phosphate isomerase	Rattus norvegicus	24-27	
1317431-9	1992	The alpha 1-adrenoceptor-mediated increase in intracellular pH (pHi) was 0.1 pH unit in HEPES buffer containing 4.4 mM-NaHCO3 and in Krebs buffer containing 25 mM-NaHCO3.	HEPES	88-93	glucose-6-phosphate isomerase	Rattus norvegicus	64-67	
1822550-4	1991	Basal pHi in HEPES-buffered solution (containing 4.4 mM-NaHCO3) was 7.08.	HEPES	13-18	glucose-6-phosphate isomerase	Rattus norvegicus	6-9	
1660595-2	1991	The pHi in the TRIS/HEPES-buffered standard solution was 7.29 +/- 0.01.	HEPES	20-25	glucose-6-phosphate isomerase	Rattus norvegicus	4-7	
2318379-7	1990	It was found that measurements of [Ca2+]i with fura 2 were influenced by shifts in pHi that occur when cells are incubated in either HEPES-buffered or CO2/HCO3- media of differing pHo values.	HEPES	133-138	glucose-6-phosphate isomerase	Rattus norvegicus	83-86	
1694402-2	1990	In cells superfused with N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid-buffered solution at pH 7.40, basal pHi was determined to be 7.15 +/- 0.13.	HEPES	25-76	glucose-6-phosphate isomerase	Rattus norvegicus	113-116	
2213591-9	1990	In bicarbonate-free (HEPES-buffered) solution the steady-state pHi was 7.37 +/- 0.02 (n = 19), significantly higher than in bicarbonate-buffered solution.	HEPES	21-26	glucose-6-phosphate isomerase	Rattus norvegicus	63-66	
2318379-8	1990	However, at any given value of pHi, the apparent [Ca2+]i measured in cells incubated in HEPES-buffered media was slightly higher than in cells incubated in CO2/HCO3- buffered media.	HEPES	88-93	glucose-6-phosphate isomerase	Rattus norvegicus	31-34	
2610271-2	1989	Intracellular pH (pHi) was acutely lowered by NH3 prepulse in HCO3(-)-free medium buffered with 6 mM N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid, and its recovery was measured thereafter under control conditions, in the presence of amiloride to inhibit Na(+)-H+ antiport, and in the presence of N-ethylmaleimide (NEM), a plasma membrane H(+)-ATPase inhibitor.	HEPES	101-152	glucose-6-phosphate isomerase	Rattus norvegicus	18-21	
2153100-3	1990	At the age of 16-20 weeks, pHi of lymphocytes suspended in a HCO3-free HEPES-buffered solution, was markedly lower in the SHR than in the WKY rats (7.07 +/- 0.02, n = 16 and 7.22 +/- 0.01, n = 15, respectively, p less than 0.001), whereas systolic blood pressure was higher in SHR than in WKY rats (175 +/- 5.0 and 105 +/- 3.0 mm Hg, respectively, p less than 0.001).	HEPES	71-76	glucose-6-phosphate isomerase	Rattus norvegicus	27-30	
2522833-3	1989	The pHi of ventricular cells bathed in HEPES-buffered medium averaged 7.30 +/- 0.02.	HEPES	39-44	glucose-6-phosphate isomerase	Rattus norvegicus	4-7	
2551179-3	1989	In N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solutions, pHi was 7.14, and intrinsic buffer capacity was inversely related to pHi.	HEPES	3-54	glucose-6-phosphate isomerase	Rattus norvegicus	83-86	
2551179-3	1989	In N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solutions, pHi was 7.14, and intrinsic buffer capacity was inversely related to pHi.	HEPES	3-54	glucose-6-phosphate isomerase	Rattus norvegicus	152-155	
2551179-3	1989	In N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solutions, pHi was 7.14, and intrinsic buffer capacity was inversely related to pHi.	HEPES	56-61	glucose-6-phosphate isomerase	Rattus norvegicus	83-86	
2551179-3	1989	In N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solutions, pHi was 7.14, and intrinsic buffer capacity was inversely related to pHi.	HEPES	56-61	glucose-6-phosphate isomerase	Rattus norvegicus	152-155	
2838077-3	1988	The pHi of cells in physiological saline buffered with Hepes (pH 7.3) at 37 degrees C was close to 7.0.	HEPES	55-60	glucose-6-phosphate isomerase	Rattus norvegicus	4-7	
2614727-5	1989	In a HEPES-buffered bathing solution of pH 7.4, pHi had a mean value of 7.16 +/- 0.05 (mean +/- S.E.M.).	HEPES	5-10	glucose-6-phosphate isomerase	Rattus norvegicus	40-42	
2614727-5	1989	In a HEPES-buffered bathing solution of pH 7.4, pHi had a mean value of 7.16 +/- 0.05 (mean +/- S.E.M.).	HEPES	5-10	glucose-6-phosphate isomerase	Rattus norvegicus	48-51	
3436883-7	1987	Because PMA, NH4Cl, methylamine, imidazole, HEPES-buffered solutions, and low-Cl- solutions can cause increases in pHi and amiloride and acetate can cause decreases in pHi, these results suggest that intracellular alkalosis and acidosis, respectively, potentiate and blunt vasoconstrictor responses to hypoxia and other stimuli in isolated rat lungs.	HEPES	44-49	glucose-6-phosphate isomerase	Rattus norvegicus	115-118	
21887217-5	2011	In the HEPES buffered Krebs-Henseleit (KH) solution, the intracellular pH (pHi) recovery from NH(4)Cl induced acidification in the cultured caput epididymal epithelium was completely inhibited by amiloride, the inhibitor of Na(+)/H(+) exchanger (NHE).	HEPES	7-12	glucose-6-phosphate isomerase	Rattus norvegicus	71-73	
21887217-5	2011	In the HEPES buffered Krebs-Henseleit (KH) solution, the intracellular pH (pHi) recovery from NH(4)Cl induced acidification in the cultured caput epididymal epithelium was completely inhibited by amiloride, the inhibitor of Na(+)/H(+) exchanger (NHE).	HEPES	7-12	glucose-6-phosphate isomerase	Rattus norvegicus	75-78	
21887217-6	2011	Immediately changing of the KH solution from HEPES buffered to HCO(3)(-) buffered would cause another pHi recovery.	HEPES	45-50	glucose-6-phosphate isomerase	Rattus norvegicus	102-105