PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 1765086-4 1991 The dissociation constant of the alpha-lactalbumin--Ca2+ complex in 50 mM Hepes containing 150 mM Na+ at pH 7.4 and 25 degrees C, was found to be 123 +/- 2 nM and 103 +/- 43 nM when determined by the competition assay and intrinsic protein fluorescence, respectively. HEPES 74-79 lactalbumin alpha Bos taurus 33-50 1765086-7 1991 The total concentration of Ca2+ in 50 mM Hepes, containing 150 mM Na+ at pH 7.4, was lowered to 119 +/- 13 nM and the number of Ca2+ bound/molecule alpha-lactalbumin was lowered to 0.069 +/- 0.006. HEPES 41-46 lactalbumin alpha Bos taurus 148-165