PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
19375489-2	2009	The ability of these self-assembled polymers to spontaneously form nano-complexes with insulin in pH 7.4 Tris buffer was evaluated by transmittance study, hydrodynamic size and zeta potential measurements.	Tromethamine	105-109	insulin	Homo sapiens	87-94	
20411195-5	2010	The chromatographically isolated Pt-insulin adducts have been proved to resist overnight digestion including treatment with Urea, DTT, IAA and trypsin in a Tris buffer.	Tromethamine	156-160	insulin	Homo sapiens	36-43	
2722845-10	1989	Therefore, the reduced formation of the tris-phosphorylated regulatory region in the diabetic receptors suggests that a defective autophosphorylation cascade leading to tris-phosphorylation of the regulatory region may cause, in part, the reduced insulin-stimulated kinase activity of the insulin receptor in muscle of NIDDM patients.	Tromethamine	40-44	insulin	Homo sapiens	247-254	
8686881-7	1995	Analysis of the concentration dependence of the electrophoretic mobility of insulin yields a lower limit for the association constant for dimerization of insulin of KD > or = 6 x 10(3) M-1 (25 mM tris and 192 mM Gly, pH 8.4).	Tromethamine	199-203	insulin	Homo sapiens	76-83	
8686881-7	1995	Analysis of the concentration dependence of the electrophoretic mobility of insulin yields a lower limit for the association constant for dimerization of insulin of KD > or = 6 x 10(3) M-1 (25 mM tris and 192 mM Gly, pH 8.4).	Tromethamine	199-203	insulin	Homo sapiens	154-161	
2722845-10	1989	Therefore, the reduced formation of the tris-phosphorylated regulatory region in the diabetic receptors suggests that a defective autophosphorylation cascade leading to tris-phosphorylation of the regulatory region may cause, in part, the reduced insulin-stimulated kinase activity of the insulin receptor in muscle of NIDDM patients.	Tromethamine	40-44	insulin	Homo sapiens	289-296	
2722845-10	1989	Therefore, the reduced formation of the tris-phosphorylated regulatory region in the diabetic receptors suggests that a defective autophosphorylation cascade leading to tris-phosphorylation of the regulatory region may cause, in part, the reduced insulin-stimulated kinase activity of the insulin receptor in muscle of NIDDM patients.	Tromethamine	169-173	insulin	Homo sapiens	247-254	
2722845-10	1989	Therefore, the reduced formation of the tris-phosphorylated regulatory region in the diabetic receptors suggests that a defective autophosphorylation cascade leading to tris-phosphorylation of the regulatory region may cause, in part, the reduced insulin-stimulated kinase activity of the insulin receptor in muscle of NIDDM patients.	Tromethamine	169-173	insulin	Homo sapiens	289-296	
3291555-1	1988	Dinitrophenol (DNP), an inhibitor of endocytosis of hormone receptors, Tris, an inhibitor of recycling and chloroquine, an inhibitor of lysosomal degradation, all decreased the binding of insulin and inhibited the development of hormonal imprinting in Tetrahymena.	Tromethamine	71-75	insulin	Homo sapiens	188-195	
3541915-6	1986	Insulin receptors were depleted by treatment of adipocytes with insulin (100 ng/ml) in the presence of Tris (which blocks receptor recycling).	Tromethamine	103-107	insulin	Homo sapiens	0-7	
3305500-10	1987	Based on these results we conclude that: 1) vanadate shunts incoming insulin from a more rapid retroendocytotic pathway to a slower degradative pathway and diverts insulin receptors from a Tris-insensitive recycling pathway to one that can be completely inhibited by Tris; 2) these effects are selective, in that vanadate impairs neither insulin degradation nor receptor uptake and recycling.	Tromethamine	267-271	insulin	Homo sapiens	164-171	
3305500-10	1987	Based on these results we conclude that: 1) vanadate shunts incoming insulin from a more rapid retroendocytotic pathway to a slower degradative pathway and diverts insulin receptors from a Tris-insensitive recycling pathway to one that can be completely inhibited by Tris; 2) these effects are selective, in that vanadate impairs neither insulin degradation nor receptor uptake and recycling.	Tromethamine	267-271	insulin	Homo sapiens	164-171	
3305500-8	1987	In related studies Tris was found to inhibit insulin-mediated receptor recycling by only 10%, whereas in the presence of vanadate (plus Tris) almost all incoming insulin receptors were prevented from recycling.	Tromethamine	19-23	insulin	Homo sapiens	45-52	
3305500-8	1987	In related studies Tris was found to inhibit insulin-mediated receptor recycling by only 10%, whereas in the presence of vanadate (plus Tris) almost all incoming insulin receptors were prevented from recycling.	Tromethamine	136-140	insulin	Homo sapiens	162-169	
3307777-3	1987	However, when insulin binding to purified liver plasma membranes was measured at 15 degrees C in 50 mM Tris, pH 7.5 containing 0.1% bovine serum albumin and 100 U/ml bacitracin, the insulin binding data was characterised by a linear Scatchard plot and a Hill plot with a slope equal to unity.	Tromethamine	103-107	insulin	Homo sapiens	14-21	
3307777-3	1987	However, when insulin binding to purified liver plasma membranes was measured at 15 degrees C in 50 mM Tris, pH 7.5 containing 0.1% bovine serum albumin and 100 U/ml bacitracin, the insulin binding data was characterised by a linear Scatchard plot and a Hill plot with a slope equal to unity.	Tromethamine	103-107	insulin	Homo sapiens	182-189	
3541915-6	1986	Insulin receptors were depleted by treatment of adipocytes with insulin (100 ng/ml) in the presence of Tris (which blocks receptor recycling).	Tromethamine	103-107	insulin	Homo sapiens	64-71	
3884607-8	1985	Receptors were efficiently recycled, with little or no net loss observed even after 4 h of insulin treatment; however, recycling could be partially inhibited (approximately 10%) by several agents (e.g. chloroquine and Tris).	Tromethamine	218-222	insulin	Homo sapiens	91-98	
3899814-1	1985	Proteolysis of insulin or (pre)proinsulin with S. aureus protease V8 in Tris buffer at neutral pH yields a characteristic pattern of peptide fragments that is resolved using high-performance liquid chromatography.	Tromethamine	72-76	insulin	Homo sapiens	31-41	
3884607-9	1985	Tris treatment of adipocytes in the presence of insulin led to 50% loss of surface and total receptors at 2 and 4 h, respectively.	Tromethamine	0-4	insulin	Homo sapiens	48-55	
3884607-11	1985	From these results we conclude that: 1) insulin triggers endocytotic uptake of insulin-receptor complexes; 2) internalized receptors are then rapidly reinserted into the plasma membrane, and the receptors can traverse this recycling pathway within 6 min; 3) prolonged recycling does not normally result in measurable receptor loss, but when receptors are prevented from recycling, they become trapped intracellularly and are shunted to a chloroquine-sensitive degradative pathway; and 4) chloroquine and Tris are only partially effective inhibitors of receptor recycling.	Tromethamine	504-508	insulin	Homo sapiens	40-47	
6582851-12	1983	The different results in the different buffers, indicate that a buffer containing Hepes and/or Tris, is required to expose negative cooperativity and make the receptors more accessible to insulin.	Tromethamine	95-99	insulin	Homo sapiens	188-195	
6363674-2	1983	The solubility of insulin may be improved with the addition of small amounts of aspartic acid, glutamic acid, EDTA (ethylenediaminetetraacetic acid), lysine, Tris buffer, or bicarbonate buffer.	Tromethamine	158-162	insulin	Homo sapiens	18-25	
1181282-5	1975	In the second procedure, the preparation of tris-Boc-[A1-leucine]insulin was accomplished by reaction of Boc-leucine N-hydroxysuccinimide ester with NalphaB1,NepsilonB29-bis(tert.-butyloxycarbonyl)-des-A1-glycine-insulin.	Tromethamine	44-48	insulin	Homo sapiens	65-72	
1181282-5	1975	In the second procedure, the preparation of tris-Boc-[A1-leucine]insulin was accomplished by reaction of Boc-leucine N-hydroxysuccinimide ester with NalphaB1,NepsilonB29-bis(tert.-butyloxycarbonyl)-des-A1-glycine-insulin.	Tromethamine	44-48	insulin	Homo sapiens	213-220	
5497467-0	1970	Effect of tri-hydroxymethyl-aminomethane (Tris) on insulin secretion in vitro.	Tromethamine	10-40	insulin	Homo sapiens	51-58	
5497467-0	1970	Effect of tri-hydroxymethyl-aminomethane (Tris) on insulin secretion in vitro.	Tromethamine	42-46	insulin	Homo sapiens	51-58