PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 22153974-1 2012 Originally, the circulating bioactivity of IGF-I was estimated by bioassays measuring the ability of serum to stimulate uptake of labeled sulfate or thymidine in cultures of costal cartilage or by the ability of serum to stimulate the uptake of glucose in fat tissue cultures. Thymidine 149-158 insulin like growth factor 1 Homo sapiens 43-48 30323899-4 2018 Cellular responses to BRCA1 silencing and IGF-I actions were investigated using western blotting, 3-H Thymidine incorporation assay, cell fractionation and co-immunoprecipitation. Thymidine 102-111 insulin like growth factor 1 Homo sapiens 42-47 27519211-12 2016 In addition, stimulation of HMCs with IGF1 alone or IGF1 complexed to wild-type, but not protease-resistant, IGF BP-4 led to increased [(3)H]-thymidine incorporation. Thymidine 142-151 insulin like growth factor 1 Homo sapiens 38-42 27519211-12 2016 In addition, stimulation of HMCs with IGF1 alone or IGF1 complexed to wild-type, but not protease-resistant, IGF BP-4 led to increased [(3)H]-thymidine incorporation. Thymidine 142-151 insulin like growth factor 1 Homo sapiens 52-56 24059861-2 2014 We demonstrate that in L6 myoblasts expressing only IGF-IRs as well as in the same cells overexpressing the IR, IGF-I (insulin-like growth factor 1), insulin and X10 (AspB10 insulin) down-regulate the mRNA expression level of the cell cycle inhibitor cyclin G2, as measured by qRT-PCR (quantitative reverse transcription-PCR), and induce cell growth measured by [6-(3)H]thymidine incorporation into DNA. Thymidine 370-379 insulin like growth factor 1 Homo sapiens 52-57 24059861-2 2014 We demonstrate that in L6 myoblasts expressing only IGF-IRs as well as in the same cells overexpressing the IR, IGF-I (insulin-like growth factor 1), insulin and X10 (AspB10 insulin) down-regulate the mRNA expression level of the cell cycle inhibitor cyclin G2, as measured by qRT-PCR (quantitative reverse transcription-PCR), and induce cell growth measured by [6-(3)H]thymidine incorporation into DNA. Thymidine 370-379 insulin like growth factor 1 Homo sapiens 119-147 23793696-14 2013 A mathematical model of circulating IGF-1 (Ac) was developed to include parameters of endogenous synthesis (Ksyn); exogenous uptake (Ka) from the subcutaneous dose (As): and IGF-1 clearance: where dAc/dt=Ksyn - KoutxAc+KaxAs. Thymidine 201-203 insulin like growth factor 1 Homo sapiens 36-41 22553932-3 2012 IGF-I resulted in significantly higher thymidine incorporation in SMC seeded on AGE-modified fibronectin (AGE-FN) in comparison to cells seeded on fibronectin (FN). Thymidine 39-48 insulin like growth factor 1 Homo sapiens 0-5 22447628-10 2012 In hGCs and KGN cells, rhChem also decreased IGF-1-induced thymidine incorporation. Thymidine 59-68 insulin like growth factor 1 Homo sapiens 45-50 20924562-8 2010 Insulin-induced stimulation of [(3)H]-thymidine incorporation was reduced by 83% after siRNA-mediated down-regulation of IGF-1 receptor by about 75%, but not affected by a similar down-regulation of the insulin receptor. Thymidine 38-47 insulin like growth factor 1 Homo sapiens 121-126 20924562-5 2010 Insulin and IGF-1 stimulated [(3)H]-thymidine incorporation in all cells. Thymidine 36-45 insulin like growth factor 1 Homo sapiens 12-17 16757531-10 2006 Consequently, the maximal [(3)H]thymidine incorporation upon challenge with a dose range of IGF-I was reduced compared with a panel of control cells (3.65 +/- 1.79-fold vs. 6.75 +/- 4.7-fold stimulation; P < 0.01). Thymidine 32-41 insulin like growth factor 1 Homo sapiens 92-97 19589910-13 2009 IGF1 and 2 at a concentration of 10(-8)-10(-7) mol/l significantly stimulated (3)H-thymidine incorporation, whereas insulin did not. Thymidine 83-92 insulin like growth factor 1 Homo sapiens 0-10 16996205-4 2007 IGF-1 stimulated thymidine incorporation was inhibited by treatment with IGFBP-3, the IGF-1R tyrosine kinase inhibitor NVP-AEW541, or the EGFR tyrosine kinase inhibitor AG1478. Thymidine 17-26 insulin like growth factor 1 Homo sapiens 0-5 19895315-5 2009 RESULTS: IGF-1 stimulated hRPE proliferation, as demonstrated by [(3)H]thymidine incorporation. Thymidine 71-80 insulin like growth factor 1 Homo sapiens 9-14 16094530-13 2005 IGF-I at 10(-9)-10(-8) mol/l significantly stimulated [3H]thymidine incorporation and at a concentration of 10(-9)-10(-7) mol/l also D-[U-14C]glucose accumulation in HCASMCs. Thymidine 58-67 insulin like growth factor 1 Homo sapiens 0-5 16030168-13 2005 Stimulation of [3H]thymidine uptake was most responsive to IGF-I; insulin and glulisine were equally less effective, with sensitivities approximately 1-2% of that for IGF-I. Thymidine 19-28 insulin like growth factor 1 Homo sapiens 59-64 15611844-5 2005 Furthermore, IGF-1 treatment induced beneficial effects on cardiac function since treated hamsters presented improved cardiac output and stroke volume, decreased end diastolic pressure when compared to nontreated hamsters and also showed a trend towards increased contractility (dP/dt(max)).This study provides evidence that IGF-1 treatment induces beneficial structural and functional effects on DCM of CHF147 hamsters, hence making this molecule a promising candidate for future gene therapy of heart failure due to DCM. Thymidine 282-284 insulin like growth factor 1 Homo sapiens 13-18 15178549-10 2004 IGFBP-3 also caused IGF-I-independent inhibition of basal [3H]thymidine incorporation. Thymidine 62-71 insulin like growth factor 1 Homo sapiens 20-25 15733404-4 2004 However, IGF-1 response was found to be about twice greater in PCOS cells at all experimental concentrations with respect to thymidine incorporation compared to control cells, a mitogenic action. Thymidine 125-134 insulin like growth factor 1 Homo sapiens 9-14 15507507-3 2005 PPFs virally transfected with Ad-IGF-I or Ad-IGF-II showed 7-fold (P < 0.01) and 3-fold (P < 0.01) increases in [(3)H]thymidine incorporation at 48 h post infection compared with nontransfected controls. Thymidine 124-133 insulin like growth factor 1 Homo sapiens 33-38 15506488-4 2004 RESULTS: [3H] thymidine incorporation into TM cells was promoted by 10 and 100 ng/ml of PDGF, IGF-1, and FGF2 after 24 and 48 hours, whereas 1, 10, and 100 ng/ml of VEGF restrained cell proliferation after 48 hours. Thymidine 14-23 insulin like growth factor 1 Homo sapiens 94-99 15329054-7 2004 Addition of a NO donor to eNOSKO cells recovered the effect of IGF-I on thymidine incorporation. Thymidine 72-81 insulin like growth factor 1 Homo sapiens 63-68 14657012-7 2004 IGF-I-stimulated [(3)H]thymidine incorporation was significantly enhanced by pretreatment with 1 nm TNFalpha, and this enhancement was blocked in the presence of protease-resistant IGFBP-4. Thymidine 23-32 insulin like growth factor 1 Homo sapiens 0-5 12932745-5 2003 IGF-I was a weak mitogen, 1.5-fold increase in [3H]thymidine incorporation, for CoSMC. Thymidine 51-60 insulin like growth factor 1 Homo sapiens 0-5 14556818-8 2003 However, IGF-1 stimulation was found to be greater in PCOS cells at all experimental concentrations with respect to thymidine incorporation compared with NO cells, which is a mitogenic action. Thymidine 116-125 insulin like growth factor 1 Homo sapiens 9-14 11998867-9 2002 Also the addition of bFGF, PDGF-AB and EGF prior to IGF-I induced a higher 3[H]thymidine uptake in all fibroblasts compared to the combination of each in reverse order. Thymidine 79-88 insulin like growth factor 1 Homo sapiens 52-57 12867429-8 2003 MCF-7 cells transiently overexpressing p21 showed increased basal and IGF-I-induced thymidine incorporation. Thymidine 84-93 insulin like growth factor 1 Homo sapiens 70-75 12763169-5 2003 IGF-I increased the [(3)H] thymidine incorporation in all Tc cells but GH did not augment this effect. Thymidine 27-36 insulin like growth factor 1 Homo sapiens 0-5 12763169-6 2003 In Gc, IGF-I stimulated [(3)H] thymidine incorporation in cells derived from SF and MF but not from LF. Thymidine 31-40 insulin like growth factor 1 Homo sapiens 7-12 12127559-3 2002 Abundant expression of IGF-I, IGF-II and IGF-IR was demonstrated in resting cells and exogenous IGF-I and IGF-II increased 3H-thymidine incorporation in a dose dependent manner. Thymidine 126-135 insulin like growth factor 1 Homo sapiens 23-28 12127559-3 2002 Abundant expression of IGF-I, IGF-II and IGF-IR was demonstrated in resting cells and exogenous IGF-I and IGF-II increased 3H-thymidine incorporation in a dose dependent manner. Thymidine 126-135 insulin like growth factor 1 Homo sapiens 30-35 11983706-3 2002 In contrast, at 25 mm glucose (HG), proliferation and thymidine incorporation were stimulated by insulin, serum, epidermal growth factor, and insulin-like growth factor 1 to a comparable extent, whereas basal levels were 25% lower than those in LG. Thymidine 54-63 insulin like growth factor 1 Homo sapiens 142-170 12572863-11 2002 An [3H]thymidine incorporation study demonstrated that platelet-derived growth factor (PDGF)-BB, basic fibroblast growth factor (bFGF), and insulin-like growth factor (IGF)-I significantly increased the uptake of [3H]thymidine into isolated SEMFs. Thymidine 7-16 insulin like growth factor 1 Homo sapiens 140-174 11691647-4 2001 IGF-I stimulated thymidine incorporation and modified the pattern of IGFBPs, decreasing the inhibitory IGFBP-4 through down-regulation of its mRNA, and increasing IGFBP-5 which, per se, was able to modulate HREC growth, exerting post-transcriptional control. Thymidine 17-26 insulin like growth factor 1 Homo sapiens 0-5 11713219-4 2001 Consistent with their changes, IGF-1-induced MAPK activation and thymidine incorporation were enhanced in WT-Shc cells, whereas they were again decreased in 3F-Shc cells. Thymidine 65-74 insulin like growth factor 1 Homo sapiens 31-36 11739448-6 2001 Thymidine uptake into DNA was stimulated foremost by IGF-I, whereas human insulin and insulin glargine showed equivalent, but greatly reduced, sensitivities and potencies (<1% IGF-I). Thymidine 0-9 insulin like growth factor 1 Homo sapiens 53-58 11739448-6 2001 Thymidine uptake into DNA was stimulated foremost by IGF-I, whereas human insulin and insulin glargine showed equivalent, but greatly reduced, sensitivities and potencies (<1% IGF-I). Thymidine 0-9 insulin like growth factor 1 Homo sapiens 179-184 11768110-9 2001 Both IGF-I and bFGF exhibited dose-dependent increases in [3H]thymidine incorporation with increasing concentration from 1 to 50 ng/mL (P < 0.05), whereas TGF-beta1 caused a dose-dependent decrease from 0.01 to 0.5 ng/mL (P < 0.05). Thymidine 62-71 insulin like growth factor 1 Homo sapiens 5-10 11052994-3 2000 IGFBP-4 inhibited IGF-I-induced [(3)H]thymidine incorporation. Thymidine 38-47 insulin like growth factor 1 Homo sapiens 18-23 11454519-6 2001 RESULTS: IGF-I and IGF-II stimulated thymidine incorporation into DNA and suppressed apoptosis in a dose-dependent manner with maximal effects at 1 and 3 nM respectively. Thymidine 37-46 insulin like growth factor 1 Homo sapiens 9-14 11472074-5 2001 IGF-I stimulated mitogenesis as measured by [(3)H]thymidine uptake experiments in U251 and U373 cells. Thymidine 50-59 insulin like growth factor 1 Homo sapiens 0-5 11281373-7 2000 Y1 cells overexpressing the human IGF-I receptor respond to IGF-I with an increase in thymidine incorporation by 140%. Thymidine 86-95 insulin like growth factor 1 Homo sapiens 34-39 10913018-1 2000 In human osteoblast-like MG-63 cells, extracellular ATP increased [(3)H]thymidine incorporation and cell proliferation and synergistically enhanced platelet-derived growth factor- or insulin-like growth factor I-induced [(3)H]thymidine incorporation. Thymidine 72-81 insulin like growth factor 1 Homo sapiens 183-211 11053478-4 2000 Its activity as an IGF-I antagonist was confirmed in an opossum kidney cell line from its inhibitory effect on the increase in thymidine incorporation into DNA induced by recombinant human IGF-I. Thymidine 127-136 insulin like growth factor 1 Homo sapiens 189-194 10913018-1 2000 In human osteoblast-like MG-63 cells, extracellular ATP increased [(3)H]thymidine incorporation and cell proliferation and synergistically enhanced platelet-derived growth factor- or insulin-like growth factor I-induced [(3)H]thymidine incorporation. Thymidine 226-235 insulin like growth factor 1 Homo sapiens 183-211 10389886-8 1999 It was found that IGF-1 increased thymidine incorporation in primary meningioma cultures in a dose-dependent manner: 1 ng/ml, 5 ng/ml, and 10 ng/ml resulted in increases in thymidine incorporation of 21%, 43%, and 176%, respectively, over baseline values. Thymidine 34-43 insulin like growth factor 1 Homo sapiens 18-23 10487672-5 1999 Stimulation of thymidine incorporation, a mitogenic action of insulin, was similar in PCOS and control fibroblasts in response to both insulin and IGF-I. Thymidine 15-24 insulin like growth factor 1 Homo sapiens 147-152 10389886-8 1999 It was found that IGF-1 increased thymidine incorporation in primary meningioma cultures in a dose-dependent manner: 1 ng/ml, 5 ng/ml, and 10 ng/ml resulted in increases in thymidine incorporation of 21%, 43%, and 176%, respectively, over baseline values. Thymidine 173-182 insulin like growth factor 1 Homo sapiens 18-23 9654167-3 1998 RESULTS: IGF-I alone resulted in a significant dose-dependent increase in medium estradiol (E2) (p<0.05) and progesterone (P) (p<0.001) and suppression of IGF-binding protein-1 (IGFBP-1) (p<0.001), without any increase in [3H]-thymidine incorporation (P=0.10). Thymidine 236-245 insulin like growth factor 1 Homo sapiens 9-14 9762008-8 1998 IGF-I and IGF-II (0.3-30 nM) dose-dependently stimulated [3H]thymidine incorporation, with a maximal response of 110% above basal. Thymidine 61-70 insulin like growth factor 1 Homo sapiens 0-5 9655695-3 1998 Growth induced by a maximally effective concentration of IGF-I (100 nM), measured as [3H]thymidine incorporation, was only partially inhibited by LY-294002 [phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor] or PD-98059 [mitogen-activated protein (MAP) kinase kinase (MEK) inhibitor] (86 +/- 7% and 35 +/- 6% inhibition, respectively) alone but was abolished by the two combined (114 +/- 18% inhibition), implying the participation of both pathways. Thymidine 89-98 insulin like growth factor 1 Homo sapiens 57-62 9886566-5 1998 Both IGF-I and -II stimulated [3H]-thymidine incorporation dose-dependently. Thymidine 35-44 insulin like growth factor 1 Homo sapiens 5-18 9876344-5 1998 Cultures of PBMCs of the patients with lipodystrophy were also used to study the effect of IGF-I stimulation on thymidine uptake in vitro. Thymidine 112-121 insulin like growth factor 1 Homo sapiens 91-96 9876344-10 1998 The effects of IGF-I stimulation on thymidine uptake by PBMCs of lipodystrophy subjects in the absence of IGFBP-1 were not different from healthy controls cultures in vitro. Thymidine 36-45 insulin like growth factor 1 Homo sapiens 15-20 9654167-4 1998 The combination of IGF-I and GH further increased the release of E2 (p<0.001), and the amount of [3H]-thymidine incorporation (p<0.001). Thymidine 105-114 insulin like growth factor 1 Homo sapiens 19-24 9437509-4 1998 Glucose (49.5 mmol/L) attenuated the increment either in DNA content or in [3H]thymidine incorporation induced by insulin-like growth factor I (IGF-I). Thymidine 79-88 insulin like growth factor 1 Homo sapiens 114-142 9437509-4 1998 Glucose (49.5 mmol/L) attenuated the increment either in DNA content or in [3H]thymidine incorporation induced by insulin-like growth factor I (IGF-I). Thymidine 79-88 insulin like growth factor 1 Homo sapiens 144-149 9348194-4 1997 The levels of HGF as well as DNA synthesis measured as thymidine incorporation were also enhanced by IGF-II and des(1-3)IGF-I, which has reduced binding to IGF binding proteins. Thymidine 55-64 insulin like growth factor 1 Homo sapiens 101-106 9430371-4 1997 Here we show that in human SMC exposed to individual growth factors (platelet-derived growth factor, epidermal growth factor, alpha-thrombin, insulin, insulin-like growth factor I (IGF-I)) and human serum, the maximal [3H]thymidine incorporation and c-fos mRNA expression are closely correlated. Thymidine 222-231 insulin like growth factor 1 Homo sapiens 151-179 8954045-7 1996 IGF-I bioactivity, as measured by thymidine incorporation by fibroblasts, did not differ in fasting and 4-h postprandial sera. Thymidine 34-43 insulin like growth factor 1 Homo sapiens 0-5 9140049-2 1997 Cells incubated in IGF-I demonstrated significant increases in thymidine incorporation (181.2 +/- 30.3% of control values; n = 12, P = 0.01) and in resting intracellular pH (pHi) (7.52 +/- 0.08 vs. 7.30 +/- 0.06; n = 20, P < 0.05), as determined by 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein quantitative microspectrofluorometry. Thymidine 63-72 insulin like growth factor 1 Homo sapiens 19-24 9056422-4 1997 We report here that overexpression of the IGF-IR did not modify sensitivity of cells to IGF-I; however, responsiveness to the ligand was moderately enhanced in most of the MCF-7/IGF-IR clones (measured by [3H]thymidine incorporation into DNA). Thymidine 209-218 insulin like growth factor 1 Homo sapiens 42-47 9218734-6 1997 In the cells in culture, cancer cells showed increased incorporation of [35S]methionine into protein and [3H]thymidine into DNA (P < 0.02) when treated with IGF-I. Thymidine 109-118 insulin like growth factor 1 Homo sapiens 160-165 8769688-4 1996 Human recombinant IGF-I and IGF-II added to cSMC cultured in a medium containing 10% fetal bovine serum (FBS) decreased the cell number and [3H]thymidine incorporation in a dose dependent fashion up to 40% and 43% compared to control cells (100%). Thymidine 144-153 insulin like growth factor 1 Homo sapiens 18-23 8768869-4 1996 Among the various cytokines, IL-6, GH, and insulin-like growth factor I (IGF-I) also enhanced Ig production and thymidine uptake in plasma cells. Thymidine 112-121 insulin like growth factor 1 Homo sapiens 43-71 8768869-4 1996 Among the various cytokines, IL-6, GH, and insulin-like growth factor I (IGF-I) also enhanced Ig production and thymidine uptake in plasma cells. Thymidine 112-121 insulin like growth factor 1 Homo sapiens 73-78 8818276-6 1996 The purified IGF-I was found to be indistinguishable from the native IGF-I by N-terminal amino acid sequence, SDS-polyacrylamide gel electrophoresis, and rp-HPLC and by biological activities such as thymidine uptake, protein synthesis and receptor binding. Thymidine 199-208 insulin like growth factor 1 Homo sapiens 13-18 8996625-8 1996 The most effective combination of growth factors, PDGF-BB (50 ng/ml) + bFGF(20 ng/ml) + IGF-1 (50 ng/ml), produced a 3H-thymidine incorporation of 44 +/- 10% (human restenosed) and 42 +/- 11% (minipig post angioplasty) of FCS values. Thymidine 120-129 insulin like growth factor 1 Homo sapiens 88-93 8774642-6 1996 RESULTS: Malignant endometrial cells increased thymidine incorporation when incubated with EGF (20.75%), TGF-alpha (19.8%), or IGF-1 (32.8%) compared to untreated control cells. Thymidine 47-56 insulin like growth factor 1 Homo sapiens 127-132 7734622-5 1995 IGF-I, forskolin and PMA each increased (P < 0.01) [3H]-thymidine incorporation in a dose and time dependent manner. Thymidine 59-68 insulin like growth factor 1 Homo sapiens 0-5 8562683-5 1995 When IGF-I activity in the GC cultures was neutralized with a specific IGF-I antibody, relaxin (10 and 100 ng/ml)-induced [3H]thymidine incorporation was inhibited (p < 0.05). Thymidine 126-135 insulin like growth factor 1 Homo sapiens 5-10 8593783-11 1996 IGF-I-induced mitogenesis, measured as thymidine incorporation and cellular proliferation, was abrogated in cells overexpressing mutant IGF-I receptors with replacement of the proximal double tyrosines (positions 1250 and 1251). Thymidine 39-48 insulin like growth factor 1 Homo sapiens 0-5 8593783-11 1996 IGF-I-induced mitogenesis, measured as thymidine incorporation and cellular proliferation, was abrogated in cells overexpressing mutant IGF-I receptors with replacement of the proximal double tyrosines (positions 1250 and 1251). Thymidine 39-48 insulin like growth factor 1 Homo sapiens 136-141 7473836-16 1995 Incorporation of [3H]thymidine into DNA in SK-ES-1 (but not MNNG/HOS) cells was increased by 25 ng/mL IGF-I (P < .01). Thymidine 21-30 insulin like growth factor 1 Homo sapiens 102-107 8562683-5 1995 When IGF-I activity in the GC cultures was neutralized with a specific IGF-I antibody, relaxin (10 and 100 ng/ml)-induced [3H]thymidine incorporation was inhibited (p < 0.05). Thymidine 126-135 insulin like growth factor 1 Homo sapiens 71-76 8544018-1 1995 This study evaluated the effects of basic fibroblast growth factor, transforming growth factor-beta 1, insulin-like growth factor-1, and insulin on the incorporation of thymidine and sulfate in human osteoarthritic articular cartilage. Thymidine 169-178 insulin like growth factor 1 Homo sapiens 36-131 7486812-7 1995 Anti-IGF-1 significantly decreased thymidine incorporation in both control cultures and those containing T. In addition, IGF-1-R-Ab blocked the expected T dependent increase in thymidine incorporation, while NS-Ab had no effect. Thymidine 35-44 insulin like growth factor 1 Homo sapiens 5-10 7486812-7 1995 Anti-IGF-1 significantly decreased thymidine incorporation in both control cultures and those containing T. In addition, IGF-1-R-Ab blocked the expected T dependent increase in thymidine incorporation, while NS-Ab had no effect. Thymidine 35-44 insulin like growth factor 1 Homo sapiens 121-126 7486812-7 1995 Anti-IGF-1 significantly decreased thymidine incorporation in both control cultures and those containing T. In addition, IGF-1-R-Ab blocked the expected T dependent increase in thymidine incorporation, while NS-Ab had no effect. Thymidine 177-186 insulin like growth factor 1 Homo sapiens 5-10 7486812-7 1995 Anti-IGF-1 significantly decreased thymidine incorporation in both control cultures and those containing T. In addition, IGF-1-R-Ab blocked the expected T dependent increase in thymidine incorporation, while NS-Ab had no effect. Thymidine 177-186 insulin like growth factor 1 Homo sapiens 121-126 7538115-11 1995 The 16-kDa IGFBP-4 fragment no longer inhibited IGF-1-stimulated thymidine uptake in vitro, suggesting that proteolytic processing of IGFBP-4 may have important functional consequences in vivo. Thymidine 65-74 insulin like growth factor 1 Homo sapiens 48-53 8817658-4 1995 30 nM IGFBP-5 stimulates 3H-thymidine incorporation in calvaria (which produce IGF I contributing to basal proliferation in serum-free medium) but not in Saos-2 cells which produce little IGF I and IGF II. Thymidine 28-37 insulin like growth factor 1 Homo sapiens 79-84 7534327-7 1995 Preincubation of fibroblasts with CM containing IGFBP-3 for 16 h before the addition of IGF-I enhanced the stimulatory effect of the hormone on thymidine incorporation into cell DNA. Thymidine 144-153 insulin like growth factor 1 Homo sapiens 88-93 8188767-11 1994 The anti-IGF-I receptor antibody alpha IR3 blocked the IGF-I and IGF-II stimulated increase of 3H-thymidine uptake. Thymidine 98-107 insulin like growth factor 1 Homo sapiens 9-14 8048522-6 1994 IGF-I stimulated [3H]thymidine incorporation and cell proliferation in a dose-dependent manner with detectable effect observed with 0.5 nM IGF-I and maximal effect at 50 nM IGF-I. Thymidine 21-30 insulin like growth factor 1 Homo sapiens 0-5 8048522-6 1994 IGF-I stimulated [3H]thymidine incorporation and cell proliferation in a dose-dependent manner with detectable effect observed with 0.5 nM IGF-I and maximal effect at 50 nM IGF-I. Thymidine 21-30 insulin like growth factor 1 Homo sapiens 139-144 8048522-6 1994 IGF-I stimulated [3H]thymidine incorporation and cell proliferation in a dose-dependent manner with detectable effect observed with 0.5 nM IGF-I and maximal effect at 50 nM IGF-I. Thymidine 21-30 insulin like growth factor 1 Homo sapiens 139-144 7517700-6 1994 However, in the presence of 2% serum, which induces half-maximal stimulation, IGF-I (100 ng/ml) further increased the rate of 3H-thymidine incorporation in stromal but not smooth muscle cells (p < 0.05). Thymidine 129-138 insulin like growth factor 1 Homo sapiens 78-83 7510688-4 1994 The two receptors bound IGF I with similar affinity (Kd approximately 1.7 nM), but the CAG- form exhibited an approximately 2-fold increase in IGF I stimulation of receptor autophosphorylation, insulin receptor substrate-1 (IRS-1) tyrosine phosphorylation, thymidine incorporation, and IRS-1-associated phosphatidylinositol 3-kinase activity. Thymidine 257-266 insulin like growth factor 1 Homo sapiens 143-148 7511802-2 1994 The addition of either IGF-I or IGF-II, but not insulin, to serum-free culture medium increases the rate of thymidine incorporation by the cells, a response which is suppressed by specific blockade of the previously described epidermal growth factor (EGF) autocrine growth regulatory loop. Thymidine 108-117 insulin like growth factor 1 Homo sapiens 23-28 8126135-7 1994 Furthermore, GH and IGF-I enhanced production of IgG1, IgG2, IgG3, IgG4, IgA1 IgA2, and IgM by and thymidine uptake of tonsillar B cells stimulated with Staphylococcus aureus Cowan strain I. Thymidine 99-108 insulin like growth factor 1 Homo sapiens 20-25 8123847-6 1994 GH and IGF-I also enhanced production of IgG1, IgG2, IgG3, IgG4, IgA1, IgA2, and IgM and thymidine uptake in PCA-1+ plasma cells generated in vitro. Thymidine 89-98 insulin like growth factor 1 Homo sapiens 7-12 7509779-7 1994 Finally, by thymidine uptake and colorimetric metabolic MTT assays, we found that most neoplastic cell lines react mitogenically to IGF-I and that its physiological effect is abolished by an anti-IGF-I-receptor antibody. Thymidine 12-21 insulin like growth factor 1 Homo sapiens 132-137 7512304-10 1994 However, pretreatment of cultured hOB with IGF-II enhanced IGF-I-stimulated [3H]thymidine incorporation threefold. Thymidine 80-89 insulin like growth factor 1 Homo sapiens 43-48 7508487-5 1993 In the absence or presence of 2.5% fetal calf serum (FCS), 100 micrograms/l of IGF-I, des(1-3)IGF-I but not long R3 IGF-I significantly stimulated thymidine incorporation into mononuclear cells. Thymidine 147-156 insulin like growth factor 1 Homo sapiens 79-84 7508487-6 1993 In addition, des(1-3)IGF-I was more potent than IGF-I in stimulating thymidine incorporation into mononuclear cells in the presence of 2.5% FCS. Thymidine 69-78 insulin like growth factor 1 Homo sapiens 21-26 7508487-6 1993 In addition, des(1-3)IGF-I was more potent than IGF-I in stimulating thymidine incorporation into mononuclear cells in the presence of 2.5% FCS. Thymidine 69-78 insulin like growth factor 1 Homo sapiens 48-53 7508487-5 1993 In the absence or presence of 2.5% fetal calf serum (FCS), 100 micrograms/l of IGF-I, des(1-3)IGF-I but not long R3 IGF-I significantly stimulated thymidine incorporation into mononuclear cells. Thymidine 147-156 insulin like growth factor 1 Homo sapiens 94-99 7508487-5 1993 In the absence or presence of 2.5% fetal calf serum (FCS), 100 micrograms/l of IGF-I, des(1-3)IGF-I but not long R3 IGF-I significantly stimulated thymidine incorporation into mononuclear cells. Thymidine 147-156 insulin like growth factor 1 Homo sapiens 94-99 7691836-10 1993 IGF-I alone (20 ng/ml) induced a 1.6 to threefold increase in 3H-thymidine incorporation. Thymidine 65-74 insulin like growth factor 1 Homo sapiens 0-5 8388881-5 1993 In contrast, the ability of insulin and IGF-I (or IGF-II) to stimulate amino acid uptake and thymidine incorporation into DNA was not impaired. Thymidine 93-102 insulin like growth factor 1 Homo sapiens 40-45 8409903-7 1993 Bovine insulin (10 micrograms/ml) and human recombinant insulin-like growth factor-I (IGF-I, 15 ng/ml) also doubled [3H]thymidine uptake in PrM cultures, but lower doses were less effective and estradiol-17 beta, transferrin, adult shark serum, purified shark relaxin, and a variety of other known growth factors were neither stimulatory nor inhibitory at the doses and conditions tested. Thymidine 120-129 insulin like growth factor 1 Homo sapiens 56-84 8409903-7 1993 Bovine insulin (10 micrograms/ml) and human recombinant insulin-like growth factor-I (IGF-I, 15 ng/ml) also doubled [3H]thymidine uptake in PrM cultures, but lower doses were less effective and estradiol-17 beta, transferrin, adult shark serum, purified shark relaxin, and a variety of other known growth factors were neither stimulatory nor inhibitory at the doses and conditions tested. Thymidine 120-129 insulin like growth factor 1 Homo sapiens 86-91 8339046-0 1993 Effect of insulin-like growth factor-I/somatomedin C on thymidine incorporation in cultured psoriatic keratinocytes after growth arrest in growth factor-free medium. Thymidine 56-65 insulin like growth factor 1 Homo sapiens 39-52 8388881-5 1993 In contrast, the ability of insulin and IGF-I (or IGF-II) to stimulate amino acid uptake and thymidine incorporation into DNA was not impaired. Thymidine 93-102 insulin like growth factor 1 Homo sapiens 50-56 8334230-3 1993 EGF, bFGF and IGF-1 partially antagonized the inhibition of cell growth and thymidine incorporation induced by TNF-alpha. Thymidine 76-85 insulin like growth factor 1 Homo sapiens 14-19 8390949-4 1993 On examination, the biological activities of insulin and insulin-like growth factor I in the patient"s cultured fibroblasts, insulin sensitivity of amino isobutyric acid uptake and thymidine incorporation was decreased, but insulin-like growth factor I action was normal. Thymidine 181-190 insulin like growth factor 1 Homo sapiens 57-85 7682287-6 1993 The IGF-I AS-Oligo also inhibited [3H]thymidine incorporation into DNA in a dose-dependent fashion (by 77% at 2 microM and by 95% at 20 microM). Thymidine 38-47 insulin like growth factor 1 Homo sapiens 4-9 8466592-4 1993 In additional studies, IGF-1 (50 ng/mL) significantly stimulated thymidine incorporation into these cells in a fashion indistinguishable from that of insulin. Thymidine 65-74 insulin like growth factor 1 Homo sapiens 23-28 8358865-4 1993 P19 cells showed an increase in thymidine incorporation of 150% of control with a 4 hour incubation of IGF I (10 ng/ml) or IGF II (100 ng/ml) and an increase in cell viability compared to control cells during 24 hours of serum starvation. Thymidine 32-41 insulin like growth factor 1 Homo sapiens 103-108 1311720-12 1992 In MCF-7 cells both IGF-I and insulin stimulated [3H]thymidine incorporation; alpha-IR3 blocked all of the IGF-I effect but only 50-60% of the insulin effect. Thymidine 53-62 insulin like growth factor 1 Homo sapiens 20-25 1332906-1 1992 Insulin-like growth factor-I (IGF-I) stimulated the growth and [3H]thymidine uptake in MCF-7 human breast cancer cells grown in serum- and growth factor-inactivated serum-containing media. Thymidine 67-76 insulin like growth factor 1 Homo sapiens 0-28 1332906-1 1992 Insulin-like growth factor-I (IGF-I) stimulated the growth and [3H]thymidine uptake in MCF-7 human breast cancer cells grown in serum- and growth factor-inactivated serum-containing media. Thymidine 67-76 insulin like growth factor 1 Homo sapiens 30-35 1325119-6 1992 IGF-I (100 pM-100 nM) stimulated the incorporation of radiolabeled alanine as a measure for total protein synthesis, 3H-labeled thymidine incorporation into DNA, and glycogen synthesis at 20 and 1% O2 in a concentration-dependent fashion. Thymidine 128-137 insulin like growth factor 1 Homo sapiens 0-5 1396882-8 1992 IGF-I from the patient showed normal bioactivities when determined by [35S]sulphate and [3H]thymidine uptake into cultured rat chondrocytes, and by [3H]thymidine and [3H]alpha-aminoisobutyric acid uptake into the patient"s skin fibroblasts. Thymidine 92-101 insulin like growth factor 1 Homo sapiens 0-5 1396882-8 1992 IGF-I from the patient showed normal bioactivities when determined by [35S]sulphate and [3H]thymidine uptake into cultured rat chondrocytes, and by [3H]thymidine and [3H]alpha-aminoisobutyric acid uptake into the patient"s skin fibroblasts. Thymidine 152-161 insulin like growth factor 1 Homo sapiens 0-5 1383262-7 1992 In conjunction with previous findings that IGF-I stimulates the uptake and incorporation of thymidine into DNA, these results suggest that IGF-I is involved in growth of human granulosa-luteal cells. Thymidine 92-101 insulin like growth factor 1 Homo sapiens 43-48 1383262-7 1992 In conjunction with previous findings that IGF-I stimulates the uptake and incorporation of thymidine into DNA, these results suggest that IGF-I is involved in growth of human granulosa-luteal cells. Thymidine 92-101 insulin like growth factor 1 Homo sapiens 139-144 1548349-6 1992 Immature granulosa cells: Tritiated thymidine uptake in granulosa cell cultures from immature follicles were significantly increased by IGF-I. Thymidine 36-45 insulin like growth factor 1 Homo sapiens 136-141 1548349-10 1992 Mature granulosa cells: IGF-I also stimulated thymidine uptake. Thymidine 46-55 insulin like growth factor 1 Homo sapiens 24-29 1548349-15 1992 Suppression of basal and IGF-I-stimulated thymidine uptake by LH was even more pronounced. Thymidine 42-51 insulin like growth factor 1 Homo sapiens 25-30 1562524-11 1992 When oestradiol (1 nM) and IGF-I (2.5 nM) were added together, the combined effect on thymidine incorporation and cell number was significantly greater than additive. Thymidine 86-95 insulin like growth factor 1 Homo sapiens 27-32 1547308-6 1992 IGF-I increased the thymidine labeling index of granulosa cells from small follicles within 24 h and enhanced cell multiplication. Thymidine 20-29 insulin like growth factor 1 Homo sapiens 0-5 1483875-4 1992 Exogenous IGF-I (20 ng/mL) stimulated [3H]thymidine incorporation in serum-free medium; approximately 70% of the IGF-I-induced stimulation was blocked by the presence of the receptor antibody. Thymidine 42-51 insulin like growth factor 1 Homo sapiens 10-15 1483875-4 1992 Exogenous IGF-I (20 ng/mL) stimulated [3H]thymidine incorporation in serum-free medium; approximately 70% of the IGF-I-induced stimulation was blocked by the presence of the receptor antibody. Thymidine 42-51 insulin like growth factor 1 Homo sapiens 113-118 1752778-3 1991 It was concluded that the tumor content of IGF-1 and the proportion of IGF-I-positive cells were inversely correlated to the tumor growth and the 3H-thymidine labeling index in vivo. Thymidine 149-158 insulin like growth factor 1 Homo sapiens 43-48 1823034-9 1991 We also measured other growth-blocking IgG that inhibited [3H]-thymidine incorporation into DNA stimulated by insulin-like growth factor-I (IGF-I), a growth factor working through a cAMP-independent pathway. Thymidine 63-72 insulin like growth factor 1 Homo sapiens 110-138 1823034-9 1991 We also measured other growth-blocking IgG that inhibited [3H]-thymidine incorporation into DNA stimulated by insulin-like growth factor-I (IGF-I), a growth factor working through a cAMP-independent pathway. Thymidine 63-72 insulin like growth factor 1 Homo sapiens 140-145 1752778-3 1991 It was concluded that the tumor content of IGF-1 and the proportion of IGF-I-positive cells were inversely correlated to the tumor growth and the 3H-thymidine labeling index in vivo. Thymidine 149-158 insulin like growth factor 1 Homo sapiens 71-76 1656551-7 1991 IGF-I either alone or in combination with HBA-71 stimulated the proliferation of thymocytes under serum-free conditions whereas in complete medium, IGF-I stimulated thymidine incorporation and the HBA-71 antibody either alone or in the presence of IGF-I showed inhibitory activity most likely due to down-regulation of the receptor. Thymidine 165-174 insulin like growth factor 1 Homo sapiens 148-153 1717510-8 1991 Changes in cell surface receptor number were reflected in cell responsiveness to IGF-I-stimulated [3H]thymidine incorporation and [3H]aminoisobutyric acid uptake. Thymidine 102-111 insulin like growth factor 1 Homo sapiens 81-86 1656551-7 1991 IGF-I either alone or in combination with HBA-71 stimulated the proliferation of thymocytes under serum-free conditions whereas in complete medium, IGF-I stimulated thymidine incorporation and the HBA-71 antibody either alone or in the presence of IGF-I showed inhibitory activity most likely due to down-regulation of the receptor. Thymidine 165-174 insulin like growth factor 1 Homo sapiens 148-153 1905506-2 1991 It was found that IGF-I alone stimulated thymidine incorporation in both types of granulosa cells. Thymidine 41-50 insulin like growth factor 1 Homo sapiens 18-23 1721920-3 1991 Half-maximal stimulation of [3H]thymidine incorporation was obtained with 10 ng/ml exogenous IGF-I, which was chosen for suppression experiments with graded amounts of purified IGFBP-1. Thymidine 32-41 insulin like growth factor 1 Homo sapiens 93-98 1707060-2 1991 Similarly, the ED50 of IGF-I-stimulated thymidine incorporation in fibroblasts from this child is 2.8 times higher than that in fibroblasts from four normal subjects. Thymidine 40-49 insulin like growth factor 1 Homo sapiens 23-28 1847964-5 1991 Human IGF-I (hIGF-I), hIGF-II and porcine insulin increased thymidine incorporation into DNA by threefold in muscle satellite cells prepared from neonatal chickens. Thymidine 60-69 insulin like growth factor 1 Homo sapiens 6-11 1847964-5 1991 Human IGF-I (hIGF-I), hIGF-II and porcine insulin increased thymidine incorporation into DNA by threefold in muscle satellite cells prepared from neonatal chickens. Thymidine 60-69 insulin like growth factor 1 Homo sapiens 13-19 2250153-2 1990 IGF-I alkalinized thyroid cells and stimulated thymidine incorporation in a dose-dependent manner; the effects of IGF-I on alkalinization (the maximal rates of change of cytoplasmic pHi/min ((dpHi/dt)max)) and thymidine incorporation were observed at 2 ng/ml and were maximal at 100 ng/ml, with half-maximal stimulation at approximately 10 ng/ml. Thymidine 47-56 insulin like growth factor 1 Homo sapiens 0-5 1846163-10 1991 In SCL-1 cells, IGF-1 was more potent than IGF-2 or insulin at lower concentrations, but either IGF-1 or IGF-2 at the maximal concentration of 333 ng/ml stimulated a 4.7-times increase in thymidine incorporation. Thymidine 188-197 insulin like growth factor 1 Homo sapiens 16-21 2175183-7 1990 Recently we found that the same IGFBP-1 preparation inhibits IGF-I-stimulated [3H]thymidine incorporation into human hyperstimulated granulosa cells. Thymidine 82-91 insulin like growth factor 1 Homo sapiens 61-66 2171510-3 1990 Surprisingly, this monoclonal antibody inhibits the ability of both IGF-I and II to stimulate thymidine synthesis in cells with the expressed receptor. Thymidine 94-103 insulin like growth factor 1 Homo sapiens 68-73 2556929-2 1989 An antibody to the human type I IGF receptor (alpha IR-3) inhibited 125I-IGF-1 binding and prevented stimulation of thymidine incorporation by IGF-1 in vitro. Thymidine 116-125 insulin like growth factor 1 Homo sapiens 143-148 2390866-5 1990 It had a 50% potency compared to human IGF-I in a radioimmunoassay using an antiserum raised against human IGF-I, stimulated the incorporation of [3H]-thymidine into DNA in cultured chick embryo myoblasts with a half-maximum effective dose of 5 ng/ml and displaced [125I]-labelled human IGF-I and IGF-II from binding sites in microsomal membranes prepared from both the chicken liver and the lactating rabbit mammary gland in a dose dependent manner. Thymidine 151-160 insulin like growth factor 1 Homo sapiens 107-112 2159761-4 1990 IGF-I and IGF-II are also mitogens for SH-SY5Y cells, as shown by a transient increase of the c-fos mRNA level, ornithin decarboxylase activity, thymidine incorporation, and, finally, cell division. Thymidine 145-154 insulin like growth factor 1 Homo sapiens 0-5 2390866-5 1990 It had a 50% potency compared to human IGF-I in a radioimmunoassay using an antiserum raised against human IGF-I, stimulated the incorporation of [3H]-thymidine into DNA in cultured chick embryo myoblasts with a half-maximum effective dose of 5 ng/ml and displaced [125I]-labelled human IGF-I and IGF-II from binding sites in microsomal membranes prepared from both the chicken liver and the lactating rabbit mammary gland in a dose dependent manner. Thymidine 151-160 insulin like growth factor 1 Homo sapiens 39-44 2390866-5 1990 It had a 50% potency compared to human IGF-I in a radioimmunoassay using an antiserum raised against human IGF-I, stimulated the incorporation of [3H]-thymidine into DNA in cultured chick embryo myoblasts with a half-maximum effective dose of 5 ng/ml and displaced [125I]-labelled human IGF-I and IGF-II from binding sites in microsomal membranes prepared from both the chicken liver and the lactating rabbit mammary gland in a dose dependent manner. Thymidine 151-160 insulin like growth factor 1 Homo sapiens 107-112 2164527-12 1990 Most glioma cell lines exhibited an IGF-I dose-dependent stimulation of thymidine incorporation into DNA, and partially purified IGF-I receptors from these cells exhibited a dose-dependent stimulation of the autophosphorylation of the beta-subunit. Thymidine 72-81 insulin like growth factor 1 Homo sapiens 36-41 2156621-6 1990 In both cell lines [3H]thymidine incorporation was enhanced by recombinant human IGF-I, 100-1000 ng/ml. Thymidine 23-32 insulin like growth factor 1 Homo sapiens 81-86 2156621-13 1990 All seven samples showed stimulation of [3H]thymidine incorporation in the presence of recombinant human IGF-I, 100-500 ng/ml. Thymidine 44-53 insulin like growth factor 1 Homo sapiens 105-110 2156796-6 1990 IGF-1, insulin, and EGF stimulated [3H]thymidine incorporation by 77, 73, and 87%, respectively. Thymidine 39-48 insulin like growth factor 1 Homo sapiens 0-5 2156796-8 1990 Alpha-IR3 inhibited stimulation of thymidine incorporation by IGF-1 and insulin but had no effect in EGF-stimulated thymidine incorporation. Thymidine 35-44 insulin like growth factor 1 Homo sapiens 62-67 2245040-5 1990 IGF-I and IGF-II promoted thymidine incorporation into cells at a half-maximal dose of 3 and 1 nM respectively, IGF-II with a maximum potency 65% of IGF-I; insulin stimulated at a half-maximum dose of 100 nM, with similar maximum effect to IGF-I, and their effects were not additive. Thymidine 26-35 insulin like growth factor 1 Homo sapiens 0-5 2245040-5 1990 IGF-I and IGF-II promoted thymidine incorporation into cells at a half-maximal dose of 3 and 1 nM respectively, IGF-II with a maximum potency 65% of IGF-I; insulin stimulated at a half-maximum dose of 100 nM, with similar maximum effect to IGF-I, and their effects were not additive. Thymidine 26-35 insulin like growth factor 1 Homo sapiens 10-15 2245040-5 1990 IGF-I and IGF-II promoted thymidine incorporation into cells at a half-maximal dose of 3 and 1 nM respectively, IGF-II with a maximum potency 65% of IGF-I; insulin stimulated at a half-maximum dose of 100 nM, with similar maximum effect to IGF-I, and their effects were not additive. Thymidine 26-35 insulin like growth factor 1 Homo sapiens 10-15 3508726-0 1986 A recombinant IGF-1 analogue stimulates [3H]thymidine incorporation in human epiphyseal growth cartilage in vitro. Thymidine 44-53 insulin like growth factor 1 Homo sapiens 14-19 3508726-2 1986 Addition of 100 ng/ml IGF-1 to articular and epiphyseal growth plate cartilage (9 months to 16 years) resulted in an increase in [3H]thymidine incorporation to a mean of 130 +/- 4% (SE) and 378 +/- 53% of controls, respectively. Thymidine 133-142 insulin like growth factor 1 Homo sapiens 22-27 2607258-7 1989 The growth of human thyroid epithelial cells was also found to be increased by IGF-I (25-100 ng/ml) added in medium plus 1% fetal calf serum as assessed by the incorporation of [3H]thymidine into DNA. Thymidine 181-190 insulin like growth factor 1 Homo sapiens 79-84 2607258-8 1989 In the presence of a monoclonal antibody to IGF-I the increase in [3H]thymidine uptake in response to IGF-I was abolished as was that seen in response to TSH. Thymidine 70-79 insulin like growth factor 1 Homo sapiens 44-49 2607258-8 1989 In the presence of a monoclonal antibody to IGF-I the increase in [3H]thymidine uptake in response to IGF-I was abolished as was that seen in response to TSH. Thymidine 70-79 insulin like growth factor 1 Homo sapiens 102-107 2530275-3 1989 MGF, pTGF-beta 1, and pTGF-beta 2 decreased mitogen-induced [3H]thymidine incorporation by 30 to 75% in a dose-dependent manner. Thymidine 64-73 insulin like growth factor 1 Homo sapiens 0-3 2808386-6 1989 IGF-I stimulates thyroid cell proliferation, stimulates thymidine incorporation, and increases cell numbers. Thymidine 56-65 insulin like growth factor 1 Homo sapiens 0-5 2693820-0 1989 Insulin and insulin-like growth factor (IGF I) modulate the effects of bTSH on 3H-thymidine incorporation in human thyroid cells in primary culture. Thymidine 82-91 insulin like growth factor 1 Homo sapiens 40-45 2464943-7 1989 Examination of the effect of IGF-1 on the cell cycle revealed that exposure of cells to both IGF-1 and platelet-derived growth factor (PDGF) led to a significant increase in 3H-thymidine incorporation into cell layers. Thymidine 177-186 insulin like growth factor 1 Homo sapiens 29-34 2693820-4 1989 We could demonstrate a considerable, dose-dependent stimulation of 3H-thymidine incorporation in the cells by bTSH that was dependent on the presence of insulin or IGF I; bTSH alone was ineffective in that respect. Thymidine 70-79 insulin like growth factor 1 Homo sapiens 164-169 2546962-6 1989 Thus, [125I]IGF binding was approximately 50%, IGF-I stimulated [3H]thymidine uptake was approximately 40% and 60% of the control value, and IGF-I-stimulated receptor autophosphorylation was reduced by 40%. Thymidine 68-77 insulin like growth factor 1 Homo sapiens 47-52 2707157-5 1989 Inhibition of IGF-I-stimulated thymidine incorporation paralleled the ability of PP12 to inhibit IGF-I binding to the surface of FRTL5. Thymidine 31-40 insulin like growth factor 1 Homo sapiens 14-19 2464943-7 1989 Examination of the effect of IGF-1 on the cell cycle revealed that exposure of cells to both IGF-1 and platelet-derived growth factor (PDGF) led to a significant increase in 3H-thymidine incorporation into cell layers. Thymidine 177-186 insulin like growth factor 1 Homo sapiens 93-98 3058540-11 1988 When the media were supplemented with growth factors, measurable increases in uptake of [3H]thymidine occurred with human epidermal growth factor (h-EGF), insulin-like growth factor-1 (IGF-1), nerve growth factor (NGF), transforming growth factor-beta (TGF-beta), bovine acidic fibroblast growth factor (baFGF), IL-1, bovine basic fibroblast growth factor (bbFGF), and Forskolin. Thymidine 92-101 insulin like growth factor 1 Homo sapiens 155-183 2540132-4 1989 Among growth factors examined, human insulin and insulin-like growth factor 1 (IGF-1), which were produced by gene engineering techniques, stimulated the growth of metastatic NL-17 and NL-44 cells as determined by thymidine incorporation and cell counts. Thymidine 214-223 insulin like growth factor 1 Homo sapiens 49-77 2540132-4 1989 Among growth factors examined, human insulin and insulin-like growth factor 1 (IGF-1), which were produced by gene engineering techniques, stimulated the growth of metastatic NL-17 and NL-44 cells as determined by thymidine incorporation and cell counts. Thymidine 214-223 insulin like growth factor 1 Homo sapiens 79-84 3058540-11 1988 When the media were supplemented with growth factors, measurable increases in uptake of [3H]thymidine occurred with human epidermal growth factor (h-EGF), insulin-like growth factor-1 (IGF-1), nerve growth factor (NGF), transforming growth factor-beta (TGF-beta), bovine acidic fibroblast growth factor (baFGF), IL-1, bovine basic fibroblast growth factor (bbFGF), and Forskolin. Thymidine 92-101 insulin like growth factor 1 Homo sapiens 185-190 2841168-2 1988 IGF-I and PDBU stimulated sulphate and thymidine incorporation by chondrocytes. Thymidine 39-48 insulin like growth factor 1 Homo sapiens 0-5 2972576-8 1988 These results indicate that 1) the defect is specific to the insulin-receptor binding in these patients, 2) insulin and IGF-I activate glucose incorporation and alpha-aminoisobutyric acid uptake mainly through their own specific receptors, but 3) the IGF-I receptor appears to have a more important role in stimulating thymidine incorporation than the insulin receptor in physiological condition or, alternatively, an unknown postreceptor process with cascade signal transmission may overcome the decreased insulin-receptor binding to produce a normal dose-response curve. Thymidine 319-328 insulin like growth factor 1 Homo sapiens 120-125 2972283-1 1988 This study investigates the effects of BP-53, the acid-stable IGF-binding subunit of the circulating 150 kDa IGF-binding protein complex, on IGF-I-stimulated thymidine incorporation by neonatal human skin fibroblasts. Thymidine 158-167 insulin like growth factor 1 Homo sapiens 141-146 2972283-2 1988 When cells were incubated for 24 h with IGF-I in serum-free medium, and thymidine incorporation studied over the final 4-h period, maximal stimulation (4- to 7-fold) was seen with 30 ng/ml IGF-I, with a half-maximal effect at 6.8 +/- 1.2 ng/ml (SD, n = 4). Thymidine 72-81 insulin like growth factor 1 Homo sapiens 189-194 2972283-3 1988 Co-incubation of IGF-I with increasing concentrations of pure BP-53 caused dose-dependent inhibition of IGF-I-stimulated thymidine incorporation, which was complete when IGF-I and BP-53 were equimolar. Thymidine 121-130 insulin like growth factor 1 Homo sapiens 17-22 2972283-3 1988 Co-incubation of IGF-I with increasing concentrations of pure BP-53 caused dose-dependent inhibition of IGF-I-stimulated thymidine incorporation, which was complete when IGF-I and BP-53 were equimolar. Thymidine 121-130 insulin like growth factor 1 Homo sapiens 104-109 2972283-3 1988 Co-incubation of IGF-I with increasing concentrations of pure BP-53 caused dose-dependent inhibition of IGF-I-stimulated thymidine incorporation, which was complete when IGF-I and BP-53 were equimolar. Thymidine 121-130 insulin like growth factor 1 Homo sapiens 104-109 2841168-5 1988 Dibutyryl cAMP increased basal and IGF-I-stimulated sulphate incorporation but inhibited but inhibited both basal and IGF-I stimulation of thymidine incorporation. Thymidine 139-148 insulin like growth factor 1 Homo sapiens 35-40 2841168-5 1988 Dibutyryl cAMP increased basal and IGF-I-stimulated sulphate incorporation but inhibited but inhibited both basal and IGF-I stimulation of thymidine incorporation. Thymidine 139-148 insulin like growth factor 1 Homo sapiens 118-123 2961773-8 1987 Incubation in the presence of alpha IR-3, a monoclonal antibody to the type I IGF receptor, inhibited the ability of both IGF-I and IGF-II to stimulate [14C]AIB uptake and [3H]thymidine incorporation in fetal and postnatal cells. Thymidine 176-185 insulin like growth factor 1 Homo sapiens 122-127 3378214-5 1988 When a monoclonal antibody (sm-1.2) raised against Sm-C/IGF-I was added into the medium, the mitogenic effect observed by both synthetic and cell-derived Sm-C/IGF-I peptide was completely abolished; the monoclonal antibody also partially inhibited the effect of 10% fetal calf serum and the thymidine incorporation observed in serum-free medium without growth factors. Thymidine 291-300 insulin like growth factor 1 Homo sapiens 56-61 3378214-5 1988 When a monoclonal antibody (sm-1.2) raised against Sm-C/IGF-I was added into the medium, the mitogenic effect observed by both synthetic and cell-derived Sm-C/IGF-I peptide was completely abolished; the monoclonal antibody also partially inhibited the effect of 10% fetal calf serum and the thymidine incorporation observed in serum-free medium without growth factors. Thymidine 291-300 insulin like growth factor 1 Homo sapiens 159-164 2945555-4 1986 However, alpha IR-3 inhibited both insulin-like growth factor I and II stimulated [3H]thymidine incorporation. Thymidine 86-95 insulin like growth factor 1 Homo sapiens 35-63 3571867-3 1987 Maximal concentrations of SM-C/IGF-I (100 ng/ml) stimulated [3H]thymidine incorporation equally in both early and late passage cells. Thymidine 64-73 insulin like growth factor 1 Homo sapiens 31-36 3498926-3 1987 In both DF and control cells, SM-C/IGF-I (50 ng/ml), insulin (100 ng/ml), and epidermal growth factor (5 ng/ml) stimulated [3H]thymidine incorporation 5-, 2-, and 6-fold, respectively. Thymidine 127-136 insulin like growth factor 1 Homo sapiens 35-40 2956085-6 1987 Furthermore, at physiological concentration of IGF-I stimulated thymidine incorporation into DNA and increased the number of cells. Thymidine 64-73 insulin like growth factor 1 Homo sapiens 47-52 3655653-5 1986 The thymidine-labelling index doubled after IGF-I supplementation. Thymidine 4-13 insulin like growth factor 1 Homo sapiens 44-49 3512592-11 1986 The actions of SM-C/IGF-I and hPL on thymidine incorporation were additive at submaximal concentrations, but not so at maximal individual concentrations. Thymidine 37-46 insulin like growth factor 1 Homo sapiens 20-25 6362310-3 1983 We have measured the binding of [125I]SM-C/IGF-I to cultured fibroblast monolayers derived from patients with Turner syndrome, and have evaluated SM-C/IGF-I stimulation of both [3H]thymidine incorporation and cell replication. Thymidine 181-190 insulin like growth factor 1 Homo sapiens 151-156 4042975-8 1985 Both human SM-C/IGF I and MSA purified from Buffalo rat liver cell-conditioned medium enhanced thymidine incorporation by growth-restricted fetal rat myoblasts. Thymidine 95-104 insulin like growth factor 1 Homo sapiens 16-21 6386833-5 1984 Somatomedin-C (Sm-C), in contrast, was found to stimulate a 320% increase in [3H]thymidine incorporation when concentrations that are present in extracellular fluids were used (i.e., 0.5-10 ng/ml). Thymidine 81-90 insulin like growth factor 1 Homo sapiens 0-13 3003744-4 1986 alpha IR-3 competitively inhibits IGF-I-mediated stimulation of [3H]thymidine incorporation into DNA. Thymidine 68-77 insulin like growth factor 1 Homo sapiens 34-39 3880762-1 1985 At concentrations of 25 ng/ml in serum-free medium, somatomedin C (SM-C) and insulin stimulated 3H-thymidine incorporation in adult human fibroblasts 4- and 1.5-fold, respectively. Thymidine 99-108 insulin like growth factor 1 Homo sapiens 52-65 6362310-5 1983 Similarly, equivalent SM-C/IGF-I stimulation of [3H]thymidine incorporation was seen in both Turner and control fibroblasts. Thymidine 52-61 insulin like growth factor 1 Homo sapiens 27-32 6362310-6 1983 In the absence of serum, SM-C/IGF-I, at a concentration of 10-25 ng/ml, stimulated thymidine incorporation 3.7-11.8-fold in Turner fibroblasts and 1.9-9.8-fold in control cells. Thymidine 83-92 insulin like growth factor 1 Homo sapiens 30-35 6362310-7 1983 In combination with 1.0% human hypopituitary serum (HHS), SM-C/IGF-I stimulated thymidine incorporation 20-70-fold in all cell lines. Thymidine 80-89 insulin like growth factor 1 Homo sapiens 63-68 6260855-2 1981 Abrogation of thymidine secretion was observed only when elicited macrophages were cultured in the presence of macrophage growth factor (MGF), whereas resident macrophages continued secreting under similar conditions. Thymidine 14-23 insulin like growth factor 1 Homo sapiens 137-140