PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 18196977-4 2008 We show that RecA bound to (dT)(16) is optimal for co-protease activity and not active as ATPase whereas the complex with (dT)(24) is competent in ATP hydrolysis but impaired as a co-protease. Thymidine 28-30 RAD51 recombinase Homo sapiens 13-17 18196977-4 2008 We show that RecA bound to (dT)(16) is optimal for co-protease activity and not active as ATPase whereas the complex with (dT)(24) is competent in ATP hydrolysis but impaired as a co-protease. Thymidine 123-125 RAD51 recombinase Homo sapiens 13-17 18196977-5 2008 Thermodynamic measurements of the equilibrium-binding properties of these complexes showed that (dT)(24) promoted a more salt sensitive complex than the one formed with (dT)(16), indicating more ionic interactions between RecA and DNA in the former. Thymidine 97-99 RAD51 recombinase Homo sapiens 222-226 18196977-5 2008 Thermodynamic measurements of the equilibrium-binding properties of these complexes showed that (dT)(24) promoted a more salt sensitive complex than the one formed with (dT)(16), indicating more ionic interactions between RecA and DNA in the former. Thymidine 170-172 RAD51 recombinase Homo sapiens 222-226 7578246-2 1995 The association requires the presence of cofactor analog adenosine 5"-O-3-thiotriphosphate (ATP gamma S) and occurs with a rate similar to that for the association of RecA to double-stranded poly(dA).poly(dT) DNA. Thymidine 205-207 RAD51 recombinase Homo sapiens 167-171 12706714-3 2003 Here, we have investigated the role of RAD51 in the repair of different types of damage induced during DNA replication with etoposide, hydroxyurea or thymidine. Thymidine 150-159 RAD51 recombinase Homo sapiens 39-44 12189171-5 2002 Furthermore, a frameshift mutation of the human RAD51 paralog XRCC2 found in the MMR-deficient uterine tumour cell line SKUT-1 can confer thymidine sensitivity when introduced into a MMR-proficient line. Thymidine 138-147 RAD51 recombinase Homo sapiens 48-53 9624163-5 1998 Similar results were obtained for the RecA.DNA complex formed with unmodified poly(dA) and oligo(dT). Thymidine 97-99 RAD51 recombinase Homo sapiens 38-42 2211610-1 1990 To probe the role of nucleotide cofactor in the binding of single-stranded DNA to recA protein, we have developed a sedimentation assay using 5"-labeled 32P-poly(dT).recA.poly(dT) complexes sediment quantitatively when centrifuged at 100,000 x g for 45 min, whereas free poly(dT) remains in the supernatant. Thymidine 162-164 RAD51 recombinase Homo sapiens 166-170 8586638-1 1995 The ability to accommodate three single-strands of DNA by each filamentous RecA-DNA complex, in the presence of the non-hydrolysable ATP analog, adenosine 5"-O-3-thiotriphosphate (ATP gamma S), was demonstrated by monitoring the fast renaturation that occurs between the complementary homopolymers, poly(dA) and poly(dT), upon sodium dodecyl sulfate (SDS)-induced dissociation of RecA. Thymidine 317-319 RAD51 recombinase Homo sapiens 75-79 8586638-1 1995 The ability to accommodate three single-strands of DNA by each filamentous RecA-DNA complex, in the presence of the non-hydrolysable ATP analog, adenosine 5"-O-3-thiotriphosphate (ATP gamma S), was demonstrated by monitoring the fast renaturation that occurs between the complementary homopolymers, poly(dA) and poly(dT), upon sodium dodecyl sulfate (SDS)-induced dissociation of RecA. Thymidine 317-319 RAD51 recombinase Homo sapiens 380-384 8586638-3 1995 The reaction was fast and complete when SDS was added to a mixture of RecA having three nucleobases per subunit each of poly(dA) and poly(dT), while the reaction was slower and incomplete in the absence of RecA. Thymidine 138-140 RAD51 recombinase Homo sapiens 70-74 8586638-5 1995 Similar fast renaturation was observed upon the addition of SDS to a mixture of RecA with three bases each of poly(dA), poly(dT), and a non-complementary DNA, poly(dC), even when one of the complementary DNAs was added last as the third strand. Thymidine 125-127 RAD51 recombinase Homo sapiens 80-84 1518050-6 1992 The DNA-base planes are essentially perpendicular to the fibre axis of the complex between RecA and dsDNA in the presence of cofactor ATP gamma S. A somewhat tilted base geometry is found for the RecA-ATP gamma S complexes with single-stranded poly(dT) and poly(d epsilon A). Thymidine 249-251 RAD51 recombinase Homo sapiens 91-95 1518050-6 1992 The DNA-base planes are essentially perpendicular to the fibre axis of the complex between RecA and dsDNA in the presence of cofactor ATP gamma S. A somewhat tilted base geometry is found for the RecA-ATP gamma S complexes with single-stranded poly(dT) and poly(d epsilon A). Thymidine 249-251 RAD51 recombinase Homo sapiens 196-200 2211610-1 1990 To probe the role of nucleotide cofactor in the binding of single-stranded DNA to recA protein, we have developed a sedimentation assay using 5"-labeled 32P-poly(dT).recA.poly(dT) complexes sediment quantitatively when centrifuged at 100,000 x g for 45 min, whereas free poly(dT) remains in the supernatant. Thymidine 162-164 RAD51 recombinase Homo sapiens 82-86 8076649-1 1994 To obtain mechanistic insights about RecA-promoted base pairing between complementary polynucleotides, the complex formation of RecA with poly(dA) and poly(dT) in the presence of ATP (and ATP-regenerating system) has been studied. Thymidine 156-158 RAD51 recombinase Homo sapiens 128-132 8076649-9 1994 Upon addition of a complementary strand, poly(dT), to a preformed filament of RecA:BPDE-poly(dA) in the presence of ATP, the fluorescence intensity slowly decreases and a change of emission profile consistent with Watson-Crick base pairing is observed. Thymidine 46-48 RAD51 recombinase Homo sapiens 78-82 2211610-1 1990 To probe the role of nucleotide cofactor in the binding of single-stranded DNA to recA protein, we have developed a sedimentation assay using 5"-labeled 32P-poly(dT).recA.poly(dT) complexes sediment quantitatively when centrifuged at 100,000 x g for 45 min, whereas free poly(dT) remains in the supernatant. Thymidine 162-165 RAD51 recombinase Homo sapiens 82-86 2211610-1 1990 To probe the role of nucleotide cofactor in the binding of single-stranded DNA to recA protein, we have developed a sedimentation assay using 5"-labeled 32P-poly(dT).recA.poly(dT) complexes sediment quantitatively when centrifuged at 100,000 x g for 45 min, whereas free poly(dT) remains in the supernatant. Thymidine 162-165 RAD51 recombinase Homo sapiens 166-170 2211610-6 1990 Our observations are rationalized by a model in which each recA protein helical filament binds two strands of poly(dT) with a stoichiometry of 3-3.5 bases/recA monomer/strand. Thymidine 115-117 RAD51 recombinase Homo sapiens 59-63 2211610-6 1990 Our observations are rationalized by a model in which each recA protein helical filament binds two strands of poly(dT) with a stoichiometry of 3-3.5 bases/recA monomer/strand. Thymidine 115-117 RAD51 recombinase Homo sapiens 155-159 31731884-6 2019 Furthermore, we generated an EGFP-RAD51 fusion under the control of HSV thymidine kinase promoter sequences yielding moderate protein expression levels comparable to endogenously expressed RAD51. Thymidine 72-81 RAD51 recombinase Homo sapiens 34-39 2148682-13 1990 These patterns are observed for recA-mediated ATP hydrolysis with either high salt concentrations or a poly(deoxythymidylic acid) [poly(dT)] cofactor, although the activation is observed at much lower ATP and ATP gamma S concentrations when poly(dT) is used. Thymidine 136-138 RAD51 recombinase Homo sapiens 32-36 6223658-6 1983 A more approximate kinetic technique indicates that recA protein binds to epsilon DNA at least one-tenth as fast as to poly(dT); the rate constant for dissociation of recA-epsilon DNA exceeds that for recA-poly(dT) by at least 30-fold. Thymidine 124-127 RAD51 recombinase Homo sapiens 52-56 6223658-6 1983 A more approximate kinetic technique indicates that recA protein binds to epsilon DNA at least one-tenth as fast as to poly(dT); the rate constant for dissociation of recA-epsilon DNA exceeds that for recA-poly(dT) by at least 30-fold. Thymidine 124-127 RAD51 recombinase Homo sapiens 167-171 6223658-6 1983 A more approximate kinetic technique indicates that recA protein binds to epsilon DNA at least one-tenth as fast as to poly(dT); the rate constant for dissociation of recA-epsilon DNA exceeds that for recA-poly(dT) by at least 30-fold. Thymidine 124-127 RAD51 recombinase Homo sapiens 167-171 6223658-6 1983 A more approximate kinetic technique indicates that recA protein binds to epsilon DNA at least one-tenth as fast as to poly(dT); the rate constant for dissociation of recA-epsilon DNA exceeds that for recA-poly(dT) by at least 30-fold. Thymidine 124-126 RAD51 recombinase Homo sapiens 52-56 6223658-6 1983 A more approximate kinetic technique indicates that recA protein binds to epsilon DNA at least one-tenth as fast as to poly(dT); the rate constant for dissociation of recA-epsilon DNA exceeds that for recA-poly(dT) by at least 30-fold. Thymidine 124-126 RAD51 recombinase Homo sapiens 167-171 6223658-6 1983 A more approximate kinetic technique indicates that recA protein binds to epsilon DNA at least one-tenth as fast as to poly(dT); the rate constant for dissociation of recA-epsilon DNA exceeds that for recA-poly(dT) by at least 30-fold. Thymidine 124-126 RAD51 recombinase Homo sapiens 167-171 25138217-12 2014 This difference could be ascribed to a higher affinity of RecA binding to DNAs carrying a thymidine dimer than to those with an abasic site. Thymidine 90-99 RAD51 recombinase Homo sapiens 58-62 18666811-8 2008 The RAD51 gene conversion pathway was involved, because [(3)H]thymidine induced RAD51 foci, and [(3)H]thymidine-induced HR was abrogated by expression of dominant negative RAD51. Thymidine 62-71 RAD51 recombinase Homo sapiens 4-9 18666811-8 2008 The RAD51 gene conversion pathway was involved, because [(3)H]thymidine induced RAD51 foci, and [(3)H]thymidine-induced HR was abrogated by expression of dominant negative RAD51. Thymidine 62-71 RAD51 recombinase Homo sapiens 80-85 18666811-8 2008 The RAD51 gene conversion pathway was involved, because [(3)H]thymidine induced RAD51 foci, and [(3)H]thymidine-induced HR was abrogated by expression of dominant negative RAD51. Thymidine 62-71 RAD51 recombinase Homo sapiens 80-85 18666811-8 2008 The RAD51 gene conversion pathway was involved, because [(3)H]thymidine induced RAD51 foci, and [(3)H]thymidine-induced HR was abrogated by expression of dominant negative RAD51. Thymidine 102-111 RAD51 recombinase Homo sapiens 4-9