PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 17102138-7 2007 ME1 knockdown decreased flux of both pools of pyruvate through PC. Pyruvic Acid 46-54 pyruvate carboxylase Rattus norvegicus 63-65 17102138-1 2007 In islet beta-cells and INS-1 cells both the high activity of malic enzyme and the correlation of insulin secretion rates with pyruvate carboxylase (PC) flux suggest that a pyruvate-malate cycle is functionally relevant to insulin secretion. Pyruvic Acid 127-135 pyruvate carboxylase Rattus norvegicus 149-151 17102138-8 2007 In contrast, ME2 knockdown affected only PC flux of the pyruvate derived from glutamate metabolism. Pyruvic Acid 56-64 pyruvate carboxylase Rattus norvegicus 41-43 15507531-6 2005 The latter is an indicator of pyruvate-malate cycle activity, indicating that most of the increased pyruvate was converted to oxaloacetate (OAA) through the PC pathway. Pyruvic Acid 30-38 pyruvate carboxylase Rattus norvegicus 157-159 16740637-2 2006 We have previously reported that glucose-stimulated insulin secretion (GSIS) is tightly correlated with pyruvate carboxylase (PC)-catalyzed anaplerotic flux into the tricarboxylic acid cycle and stimulation of pyruvate cycling activity. Pyruvic Acid 104-112 pyruvate carboxylase Rattus norvegicus 126-128 16740637-10 2006 We conclude that beta-cells activate compensatory mechanisms in response to suppression of PC expression that prevent impairment of anaplerosis, pyruvate cycling, NAPDH production, and GSIS. Pyruvic Acid 145-153 pyruvate carboxylase Rattus norvegicus 91-93 9375662-5 1997 Increasing extracellular pyruvate from 0 to 5 mM increased pyruvate carboxylase flux as observed by increases in the 14C incorporated into pyruvate and citric acid cycle intermediates, but incorporation into glutamate and glutamine, although relatively high at low pyruvate levels, did not increase as pyruvate carboxylase flux increased. Pyruvic Acid 25-33 pyruvate carboxylase Rattus norvegicus 59-79 11448844-13 2001 The results of this study suggest that the increased gluconeogenic flux observed with HF and SU diets is associated with an increased pyruvate flux through pyruvate carboxylase and PEPCK. Pyruvic Acid 134-142 pyruvate carboxylase Rattus norvegicus 156-176 9375662-5 1997 Increasing extracellular pyruvate from 0 to 5 mM increased pyruvate carboxylase flux as observed by increases in the 14C incorporated into pyruvate and citric acid cycle intermediates, but incorporation into glutamate and glutamine, although relatively high at low pyruvate levels, did not increase as pyruvate carboxylase flux increased. Pyruvic Acid 25-33 pyruvate carboxylase Rattus norvegicus 302-322 9375662-5 1997 Increasing extracellular pyruvate from 0 to 5 mM increased pyruvate carboxylase flux as observed by increases in the 14C incorporated into pyruvate and citric acid cycle intermediates, but incorporation into glutamate and glutamine, although relatively high at low pyruvate levels, did not increase as pyruvate carboxylase flux increased. Pyruvic Acid 59-67 pyruvate carboxylase Rattus norvegicus 302-322 9375662-5 1997 Increasing extracellular pyruvate from 0 to 5 mM increased pyruvate carboxylase flux as observed by increases in the 14C incorporated into pyruvate and citric acid cycle intermediates, but incorporation into glutamate and glutamine, although relatively high at low pyruvate levels, did not increase as pyruvate carboxylase flux increased. Pyruvic Acid 59-67 pyruvate carboxylase Rattus norvegicus 302-322 9375662-7 1997 When 25 mM bicarbonate plus 0.5 mM pyruvate increased pyruvate carboxylase flux to approximately the same extent as 15 mM bicarbonate plus 5 mM pyruvate, the rate of appearance of [14C] glutamate and glutamine was higher with the lower level of pyruvate. Pyruvic Acid 35-43 pyruvate carboxylase Rattus norvegicus 54-74 8611024-6 1996 The partitioning of pyruvate between pyruvate carboxylase and pyruvate dehydrogenase was increase 2.8-fold in the periportal cells compared to that in the perivenous cells and it is suggested that this, together with possible alterations in phosphoenolpyruvate carboxykinase, is primarily responsible for the different gluconeogenic rates in the two zones of the liver. Pyruvic Acid 20-28 pyruvate carboxylase Rattus norvegicus 37-57 8074207-3 1994 Substrate cycling from phosphoenolpyruvate to pyruvate [via pyruvate kinase (PK)] and from oxaloacetate to pyruvate [via malic enzyme (ME)] relative to the pyruvate carboxylase (PC) flux [i.e., (PK+ME)/PC] was assessed by the ratio of the 13C enrichment of C-2 alanine relative to that in C-5 glucose. Pyruvic Acid 34-42 pyruvate carboxylase Rattus norvegicus 156-176 7722517-1 1995 Pyruvate carboxylase (EC 6.4.1.1; PC) catalyzes the formation of oxaloacetate by energy-dependent fixation of CO2 to pyruvate. Pyruvic Acid 117-125 pyruvate carboxylase Rattus norvegicus 0-20 8074207-3 1994 Substrate cycling from phosphoenolpyruvate to pyruvate [via pyruvate kinase (PK)] and from oxaloacetate to pyruvate [via malic enzyme (ME)] relative to the pyruvate carboxylase (PC) flux [i.e., (PK+ME)/PC] was assessed by the ratio of the 13C enrichment of C-2 alanine relative to that in C-5 glucose. Pyruvic Acid 46-54 pyruvate carboxylase Rattus norvegicus 156-176 3818490-8 1987 Neither butyrate nor acetate accelerated gluconeogenesis from propionate while acetate increased glucose synthesis from pyruvate, presumably through activation of pyruvate carboxylase. Pyruvic Acid 120-128 pyruvate carboxylase Rattus norvegicus 163-183 3284880-6 1988 [2-13C]Pyruvate was used to prove that steady-state flux of pyruvate through pyruvate carboxylase is significant during co-perfusion of pyruvate and acetate, and we demonstrate for the first time that a nine-line 13C multiplet may be detected in an intact, beating heart. Pyruvic Acid 60-68 pyruvate carboxylase Rattus norvegicus 77-97 3932072-12 1985 Glucagon favours the partitioning of pyruvate towards carboxylation, by increasing the flux through pyruvate carboxylase, without directly inhibiting the flux through PDH. Pyruvic Acid 37-45 pyruvate carboxylase Rattus norvegicus 100-120 21074-2 1977 The inhibition of pyruvate carboxylation was observed with both intact mitochondria and with the solubilized pyruvate carboxylase. Pyruvic Acid 18-26 pyruvate carboxylase Rattus norvegicus 109-129 20001964-1 2010 Pyruvate carboxylase is an enzyme of the so-called pyruvate cycling pathways, which have been proposed to contribute to glucose-stimulated insulin secretion in pancreatic beta-cells. Pyruvic Acid 51-59 pyruvate carboxylase Rattus norvegicus 0-20 4725035-15 1973 The results suggest that the metabolism of pyruvate via pyruvate carboxylase in brain mitochondria is regulated, in part, by the intramitochondrial concentrations of pyruvate, oxaloacetate and the ATP:ADP ratio. Pyruvic Acid 43-51 pyruvate carboxylase Rattus norvegicus 56-76 4634834-8 1972 The K(m) for pyruvate of rat brain pyruvate carboxylase was about 0.2mm. Pyruvic Acid 13-21 pyruvate carboxylase Rattus norvegicus 35-55 4634834-10 1972 The concentration of phenylpyruvate required for a 50% inhibition of H(14)CO(3) (-) fixation by the intact mitochondria and of pyruvate carboxylase activity was dependent on the concentration of pyruvate used in the incubation medium. Pyruvic Acid 27-35 pyruvate carboxylase Rattus norvegicus 127-147