PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 12700414-1 2003 Previously we showed that ethanol (EtOH) consumption suppressed IL-2-induced cytolytic activity of murine splenic natural killer (NK) cells. Ethanol 26-33 interleukin 2 Mus musculus 64-68 12700414-1 2003 Previously we showed that ethanol (EtOH) consumption suppressed IL-2-induced cytolytic activity of murine splenic natural killer (NK) cells. Ethanol 35-39 interleukin 2 Mus musculus 64-68 12700414-3 2003 In this study we show by electrophoretic mobility-shift assay (EMSA) that enriched splenic NK cells from EtOH-consuming C57BL/6 mice exhibit reduced NF-kappaB and AP-1 binding activity in response to IL-2 stimulation as compared to the water-drinking mice. Ethanol 105-109 interleukin 2 Mus musculus 200-204 12700414-4 2003 Semiquantitative RT-PCR and real-time PCR analyses indicated that EtOH consumption inhibits the induction of perforin, granzyme A, and granzyme B in response to IL-2. Ethanol 66-70 interleukin 2 Mus musculus 161-165 12700414-8 2003 The findings further suggest that the decreased cytolytic activity of IL-2-stimulated NK cytolytic activity in EtOH-consuming mice is due at least in part to impaired transactivation of these and possibly other genes involved in control of NK-cell target lysis. Ethanol 111-115 interleukin 2 Mus musculus 70-74 9279687-3 1997 The groups of melanoma-burdened mice treated with both local ethanol injection and local or intra-abdominal administration of IL-2 showed clear suppression of any recurrence of melanoma once the melanomas had been destroyed by ethanol injection and a concomitant prolongation of the survival times. Ethanol 227-234 interleukin 2 Mus musculus 126-130 10924011-6 2000 Furthermore, we determined whether murine interleukin-2 coadministration with the NS5 expression plasmid would reverse the inhibitory effects of chronic ethanol consumption; again, partial restoration was observed for B-cell and CD4+ T-cell activity, but not for cytotoxic T cells. Ethanol 153-160 interleukin 2 Mus musculus 42-55 10946161-1 2000 We have previously reported that ethanol (EtOH) decreases polyinosinic-polycytidylic acid (poly I:C) and interleukin-2 (IL-2)-induced upregulation of natural killer (NK) cell lytic activity in mice. Ethanol 33-40 interleukin 2 Mus musculus 105-118 10946161-1 2000 We have previously reported that ethanol (EtOH) decreases polyinosinic-polycytidylic acid (poly I:C) and interleukin-2 (IL-2)-induced upregulation of natural killer (NK) cell lytic activity in mice. Ethanol 33-40 interleukin 2 Mus musculus 120-124 10946161-1 2000 We have previously reported that ethanol (EtOH) decreases polyinosinic-polycytidylic acid (poly I:C) and interleukin-2 (IL-2)-induced upregulation of natural killer (NK) cell lytic activity in mice. Ethanol 42-46 interleukin 2 Mus musculus 105-118 10946161-1 2000 We have previously reported that ethanol (EtOH) decreases polyinosinic-polycytidylic acid (poly I:C) and interleukin-2 (IL-2)-induced upregulation of natural killer (NK) cell lytic activity in mice. Ethanol 42-46 interleukin 2 Mus musculus 120-124 9366440-7 1997 Furthermore, we determined if coadministration of an IL-2 or GM-CSF DNA expression plasmid with a plasmid expressing the HCV core protein (pHCV2-2) would reverse the inhibitory effects of chronic ethanol feeding on cellular immune responses. Ethanol 196-203 interleukin 2 Mus musculus 53-57 9366440-8 1997 Coimmunization of chronic ethanol-fed mice with either IL-2 or GM-CSF expression plasmids restored cellular immunity and induced CD4+ inflammatory T cell and CD8+ CTL responses comparable with control mice immunized with pHCV2-2 alone. Ethanol 26-33 interleukin 2 Mus musculus 55-59 12685351-3 2002 RESULTS: n-Butanol fraction of an ethanol extract of air-dried herbs bilaterally regulated the IL-2 production from splenocytes induced by ConA, and enhanced the phagocytic activities of activated mouse peritoneal macrophages. Ethanol 34-41 interleukin 2 Mus musculus 95-99 9279688-2 1997 Although ethanol injection in combination with microwaval hyperthermia and systemic administration of interleukin-2 was found to be very effective in suppressing the growth of B16 melanoma nodules with a size of less than 7 mm in diameter in our previous experiments, the growth of B16 melanomas with a size of greater than 10 mm in diameter was very difficult to control with any of the therapeutic modalities examined. Ethanol 9-16 interleukin 2 Mus musculus 102-115 8796450-8 1996 Finally, we present novel evidence that addition of exogenous IL-2 completely restores the impaired proliferative response of thymocytes from ethanol-fed mice to control levels. Ethanol 142-149 interleukin 2 Mus musculus 62-66 8819519-4 1996 In the present study, in vivo administration of EtOH also decreased interleukin-2-induced splenic NK cell activity measured 12 hr after administration of EtOH and interleukin-2. Ethanol 48-52 interleukin 2 Mus musculus 68-81 8819519-4 1996 In the present study, in vivo administration of EtOH also decreased interleukin-2-induced splenic NK cell activity measured 12 hr after administration of EtOH and interleukin-2. Ethanol 48-52 interleukin 2 Mus musculus 163-176 8819519-4 1996 In the present study, in vivo administration of EtOH also decreased interleukin-2-induced splenic NK cell activity measured 12 hr after administration of EtOH and interleukin-2. Ethanol 154-158 interleukin 2 Mus musculus 68-81 8658518-0 1996 Ethanol consumption suppresses the IL2-induced proliferation of NK cells. Ethanol 0-7 interleukin 2 Mus musculus 35-38 8658518-6 1996 There were fewer cells (p < 0.02) from ethanol-consuming mice compared to cells from water-drinking control mice after incubation with IL2 at 2, 4, and 6 days of culture. Ethanol 42-49 interleukin 2 Mus musculus 138-141 8658518-10 1996 Suppression of IL2-induced NK cell proliferation is associated with ethanol consumption, and suppression is not due to altered IL2 receptor expression, increased apoptosis, intracellular cytokine levels, or c-myc expression. Ethanol 68-75 interleukin 2 Mus musculus 15-18 8796450-10 1996 We also report that addition of exogenous IL-2 is sufficient to restore full proliferative capacity to thymocytes from ethanol-fed mice. Ethanol 119-126 interleukin 2 Mus musculus 42-46 8594302-4 1996 Pycnogenol enhanced in vitro IL-2 production by mitogen-stimulated splenocytes if its production was suppressed in ethanol-fed or retrovirus-infected mice. Ethanol 115-122 interleukin 2 Mus musculus 29-33 8935625-5 1996 Biological response modifiers such as levamisole or interleukin (IL)-2 were effective in preventing the recurrence of murine melanoma after ethanol injection. Ethanol 140-147 interleukin 2 Mus musculus 52-70 7473187-6 1995 In vivo administration of EtOH caused substantial decreases in the SRBC-induced expression of interleukin (IL)-1-beta, IL-2 and IL-4 mRNA in the spleen, as indicated by reverse transcription-polymerase chain reaction. Ethanol 26-30 interleukin 2 Mus musculus 119-123 7943666-5 1994 Cytolytic activity of enriched NK cells from ethanol-consuming mice was stimulated to levels equal to control water-drinking mice after 16- to 18-hr incubation in 1000 units/ml recombinant interleukin 2. Ethanol 45-52 interleukin 2 Mus musculus 189-202 7625567-0 1995 Acetaldehyde-serum protein adducts inhibit interleukin-2 secretion in concanavalin A-stimulated murine splenocytes: a potential common pathway for ethanol-induced immunomodulation. Ethanol 147-154 interleukin 2 Mus musculus 43-56 7625567-12 1995 Therefore, immunological effects ascribed to ethanol may result in part from the toxic properties of acetaldehyde-protein adducts on IL-2 secretion. Ethanol 45-52 interleukin 2 Mus musculus 133-137 7818792-8 1994 The production of IL-2 and IL-6 by thymocytes, which was lessened during retrovirus infection, were significantly further suppressed by dietary EtOH at 6 weeks postinfection, whereas levels of IL-4 and IFN-gamma by thymocytes, which were elevated during retrovirus infection, were significantly and slightly further increased by EtOH-treated mice prior to retrovirus infection, respectively. Ethanol 144-148 interleukin 2 Mus musculus 18-22 7818792-8 1994 The production of IL-2 and IL-6 by thymocytes, which was lessened during retrovirus infection, were significantly further suppressed by dietary EtOH at 6 weeks postinfection, whereas levels of IL-4 and IFN-gamma by thymocytes, which were elevated during retrovirus infection, were significantly and slightly further increased by EtOH-treated mice prior to retrovirus infection, respectively. Ethanol 329-333 interleukin 2 Mus musculus 18-22 8048738-7 1994 Dietary vitamin E, furthermore, significantly increased interleukin-2 and -6 secretion by Con A-stimulated thymocytes, which were suppressed by dietary EtOH, although it had no effect on interleukin-4 and interferon-gamma production by Con A-stimulated thymocytes from EtOH-fed mice. Ethanol 152-156 interleukin 2 Mus musculus 56-76 7625574-2 1995 This reduction is transient because the levels of NK cell cytotoxicity from ethanol-consuming mice are nearly equal to those of water-drinking mice after splenocytes were incubated in 1000 IU/ml of recombinant interleukin-2 (rIL2) for 16-18 hr. Ethanol 76-83 interleukin 2 Mus musculus 210-223 1521938-6 1992 The results showed that both lectin- and PMA/ionomycin-induced mitogenesis and IL2-dependent proliferation of T-cells from ethanol diet-fed mice were diminished as compared with that of maltose-substitute diet or standard liquid diet. Ethanol 123-130 interleukin 2 Mus musculus 79-82 8198229-9 1994 The levels of IL-2, IL-4, and IL-6 produced by Con A-stimulated thymocytes were significantly reduced by dietary ETOH compared with control, whereas production of IFN-gamma by thymocytes was not affected. Ethanol 113-117 interleukin 2 Mus musculus 14-18 8407049-11 1993 Because evidence indicates that (1) a normal level of [Ca2+]i increase is a prerequisite for the production of IL2 by mitogen-stimulated T-cells, and (2) T-cells from ethanol-fed mice have normal capacities to produce IL2 that is the crucial growth factor controlling T-cells to progress through the cell cycle, these lines of evidence taken together with the results of this study suggest that the impairment in [Ca2+]i increases in T-cells from ethanol-fed mice may not be the primary factor contributing to the diminished T-cell proliferation in the same mice. Ethanol 167-174 interleukin 2 Mus musculus 111-114 8407049-11 1993 Because evidence indicates that (1) a normal level of [Ca2+]i increase is a prerequisite for the production of IL2 by mitogen-stimulated T-cells, and (2) T-cells from ethanol-fed mice have normal capacities to produce IL2 that is the crucial growth factor controlling T-cells to progress through the cell cycle, these lines of evidence taken together with the results of this study suggest that the impairment in [Ca2+]i increases in T-cells from ethanol-fed mice may not be the primary factor contributing to the diminished T-cell proliferation in the same mice. Ethanol 167-174 interleukin 2 Mus musculus 218-221 1521938-9 1992 These results taken together indicate that ethanol suppresses T-cell proliferation by interfering with events following the IL2-IL2R interaction. Ethanol 43-50 interleukin 2 Mus musculus 124-127 2051788-7 1991 The results revealed that both T cell mitogenesis and IL2-dependent proliferation of T blast cells from young and old ethanol diet-fed mice were remarkably diminished as compared to that of young and old maltose-substituted diet (isocaloric control) fed mice, respectively. Ethanol 118-125 interleukin 2 Mus musculus 54-57 1431420-2 1992 We tested the hypothesis that ethanol can inhibit the effect of interleukins on B-cell proliferation by adding ethanol to in vitro functional assays for IL-2 and IL-4. Ethanol 30-37 interleukin 2 Mus musculus 153-157 2051788-8 1991 The ability of T cells from both young and old ethanol diet-fed mice to produce IL2, however, was not affected. Ethanol 47-54 interleukin 2 Mus musculus 80-83 2679200-7 1989 Splenic baseline and IL-2 stimulated NK cell activity were significantly lower in ethanol-consuming groups compared to control groups after the 1- and 2-week test periods. Ethanol 82-89 interleukin 2 Mus musculus 21-25 2190487-9 1990 Furthermore, ethanol had only a minimal suppressive effect on IL2 production by T cells of both young and old mice, even at the concentration of 100 mM. Ethanol 13-20 interleukin 2 Mus musculus 62-65 2190487-10 1990 These findings would suggest that the ethanol-mediated suppression of T cell proliferation of both young and old mice is more likely due to an impairment of metabolic event(s) associated with or subsequent to the interaction of IL2 and IL2 receptor leading to cellular replication. Ethanol 38-45 interleukin 2 Mus musculus 228-231 2190487-10 1990 These findings would suggest that the ethanol-mediated suppression of T cell proliferation of both young and old mice is more likely due to an impairment of metabolic event(s) associated with or subsequent to the interaction of IL2 and IL2 receptor leading to cellular replication. Ethanol 38-45 interleukin 2 Mus musculus 236-239 2606000-5 1989 Interleukin 2 synthesis by murine splenocytes was inhibited by ETH in a concentration-related manner; the lowest concentrations of ETH caused an increase in interleukin 2 synthesis. Ethanol 63-66 interleukin 2 Mus musculus 0-13 2606000-5 1989 Interleukin 2 synthesis by murine splenocytes was inhibited by ETH in a concentration-related manner; the lowest concentrations of ETH caused an increase in interleukin 2 synthesis. Ethanol 131-134 interleukin 2 Mus musculus 0-13 2606000-5 1989 Interleukin 2 synthesis by murine splenocytes was inhibited by ETH in a concentration-related manner; the lowest concentrations of ETH caused an increase in interleukin 2 synthesis. Ethanol 131-134 interleukin 2 Mus musculus 157-170 34482318-12 2022 This was accompanied by increased levels of IL-10 and decreased levels of pro-proliferative cytokine IL-2 in cultures containing ethanol + burn Tregs compared to sham Tregs. Ethanol 129-136 interleukin 2 Mus musculus 101-105 22634720-2 2012 We have shown previously that acute alcohol/ethanol (EtOH) intoxication combined with burn injury suppresses mesenteric lymph node (MLN) T-cell interleukin-2 (IL-2) and interferon gamma (IFN-gamma) production. Ethanol 44-51 interleukin 2 Mus musculus 144-157 3499687-4 1987 The thymuses of fetal mice exposed prenatally to ethanol were reduced both in cell number and proliferative response to Concanavalin A plus a source of interleukin 2. Ethanol 49-56 interleukin 2 Mus musculus 152-165 22634720-2 2012 We have shown previously that acute alcohol/ethanol (EtOH) intoxication combined with burn injury suppresses mesenteric lymph node (MLN) T-cell interleukin-2 (IL-2) and interferon gamma (IFN-gamma) production. Ethanol 44-51 interleukin 2 Mus musculus 159-163 22634720-2 2012 We have shown previously that acute alcohol/ethanol (EtOH) intoxication combined with burn injury suppresses mesenteric lymph node (MLN) T-cell interleukin-2 (IL-2) and interferon gamma (IFN-gamma) production. Ethanol 53-57 interleukin 2 Mus musculus 144-157 22634720-2 2012 We have shown previously that acute alcohol/ethanol (EtOH) intoxication combined with burn injury suppresses mesenteric lymph node (MLN) T-cell interleukin-2 (IL-2) and interferon gamma (IFN-gamma) production. Ethanol 53-57 interleukin 2 Mus musculus 159-163 21267811-3 2011 Oral administration of the 50 % ethanol extract at 100 mg/kg/day significantly enhanced the production of the Th1 cytokines IL-2 and IFN- gamma irrespective of concanavalin-A stimulation, whereas the production of the Th2 cytokines IL-4 and IL-5 was not affected. Ethanol 32-39 interleukin 2 Mus musculus 124-128 18387131-4 2008 Lowering of serum levels of interleukin-2 (IL-2), spleen natural killer (NK) cell activity, and serum antioxidant activity in mice with allergic symptoms were also prevented by oral administration of the ethanol extract. Ethanol 204-211 interleukin 2 Mus musculus 28-41 18387131-4 2008 Lowering of serum levels of interleukin-2 (IL-2), spleen natural killer (NK) cell activity, and serum antioxidant activity in mice with allergic symptoms were also prevented by oral administration of the ethanol extract. Ethanol 204-211 interleukin 2 Mus musculus 43-47 21799689-7 2011 Co-treatment with a 50-75% ethanol-partitioned fraction of the tuber extract of D. batatas (DsCE-II) and interleukin (IL)-2 resulted in a significantly higher rate of murine splenocyte cell proliferation ex vivo than treatment with DsCE-II or IL-2 alone. Ethanol 27-34 interleukin 2 Mus musculus 243-247 17877144-4 2007 The activities of transaminase (AST and ALT) enzymes, interleukin (IL)-10 and gamma interferon (IFN-gamma) elevated, while IL-2 and IL-4 reduced in mice serum due to ethanol exposure. Ethanol 166-173 interleukin 2 Mus musculus 123-127