PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 19732351-6 2009 Expression of nitrate transporters AtNRT1.1 and AtNRT2.1 was upregulated and downregulated in response to high nitrate concentration, respectively. Nitrates 14-21 nitrate transporter 2:1 Arabidopsis thaliana 48-56 20444232-3 2010 A region from positions -188 to -1, relative to the translation start site, was found to contain at least one cis-element necessary for the nitrate-dependent activation of the promoter, in which the activity of nitrate transporter NRT2.1 and/or NRT2.2 plays a critical role. Nitrates 140-147 nitrate transporter 2:1 Arabidopsis thaliana 231-237 20444232-3 2010 A region from positions -188 to -1, relative to the translation start site, was found to contain at least one cis-element necessary for the nitrate-dependent activation of the promoter, in which the activity of nitrate transporter NRT2.1 and/or NRT2.2 plays a critical role. Nitrates 140-147 nitrate transporter 2:1 Arabidopsis thaliana 231-235 20561257-1 2010 AtNRT2.1, a polypeptide of the Arabidopsis thaliana two-component inducible high-affinity nitrate transport system (IHATS), is located within the plasma membrane. Nitrates 90-97 nitrate transporter 2:1 Arabidopsis thaliana 0-8 20561257-2 2010 The monomeric form of AtNRT2.1 has been reported to be the most abundant form, and was suggested to be the form that is active in nitrate transport. Nitrates 130-137 nitrate transporter 2:1 Arabidopsis thaliana 22-30 20561257-9 2010 This result, together with the finding that the oligomer is absent from NRT2.1 or NAR2.1 mutants, suggests that this complex, rather than monomeric AtNRT2.1, is the form that is active in IHATS nitrate transport. Nitrates 194-201 nitrate transporter 2:1 Arabidopsis thaliana 148-156 20561257-10 2010 The molecular mass of the intact oligomer suggests that the functional unit for high-affinity nitrate influx may be a tetramer consisting of two subunits each of AtNRT2.1 and AtNAR2.1. Nitrates 94-101 nitrate transporter 2:1 Arabidopsis thaliana 162-170 19732351-7 2009 A similar upregulation and downregulation of AtNRT1.1 and AtNRT2.1 was observed by ethylene synthesis precursor aminocyclopropane carboxylic acid (ACC) and AVG in low and high nitrate concentration, respectively. Nitrates 176-183 nitrate transporter 2:1 Arabidopsis thaliana 58-66 17573350-0 2007 Regulation of root nitrate uptake at the NRT2.1 protein level in Arabidopsis thaliana. Nitrates 19-26 nitrate transporter 2:1 Arabidopsis thaliana 41-47 18713378-4 2008 The present study identified four putative NRT2 and two putative NAR2 genes that encode components of the high-affinity nitrate transport system (HATS) in the rice (Oryza sativa L. subsp. Nitrates 120-127 nitrate transporter 2:1 Arabidopsis thaliana 43-47 17573350-1 2007 In Arabidopsis the NRT2.1 gene encodes a main component of the root high-affinity nitrate uptake system (HATS). Nitrates 82-89 nitrate transporter 2:1 Arabidopsis thaliana 19-25 17481610-5 2007 Two of the NRT1 genes, CHL1 and AtNRT1.2, and two of the NRT2 genes, AtNRT2.1 and AtNRT2.2, are known to be involved in nitrate uptake. Nitrates 120-127 nitrate transporter 2:1 Arabidopsis thaliana 57-61 19704673-3 2007 Using heterologous expression in yeast and oocytes we showed that the two Arabidopsis AtNRT2.1 and AtNAR2.1 proteins interacted to give a functional high affinity nitrate transport system (HATS). Nitrates 163-170 nitrate transporter 2:1 Arabidopsis thaliana 86-94 17481610-5 2007 Two of the NRT1 genes, CHL1 and AtNRT1.2, and two of the NRT2 genes, AtNRT2.1 and AtNRT2.2, are known to be involved in nitrate uptake. Nitrates 120-127 nitrate transporter 2:1 Arabidopsis thaliana 69-77 17481610-5 2007 Two of the NRT1 genes, CHL1 and AtNRT1.2, and two of the NRT2 genes, AtNRT2.1 and AtNRT2.2, are known to be involved in nitrate uptake. Nitrates 120-127 nitrate transporter 2:1 Arabidopsis thaliana 69-75 17085507-7 2007 Disruption of NRT2.2 in Atnrt2.2 reduced IHATS by 19% and this reduction was statistically significant only at 6 h after resupply of nitrate to nitrogen-deprived plants. Nitrates 133-140 nitrate transporter 2:1 Arabidopsis thaliana 14-18 17540716-1 2007 In higher plants, nitrate is taken up by root cells where Arabidopsis thaliana NITRATE TRANSPORTER2.1 (ATNRT2.1) chiefly acts as the high-affinity nitrate uptake system. Nitrates 18-25 nitrate transporter 2:1 Arabidopsis thaliana 79-101 17540716-1 2007 In higher plants, nitrate is taken up by root cells where Arabidopsis thaliana NITRATE TRANSPORTER2.1 (ATNRT2.1) chiefly acts as the high-affinity nitrate uptake system. Nitrates 147-154 nitrate transporter 2:1 Arabidopsis thaliana 79-101 17085507-7 2007 Disruption of NRT2.2 in Atnrt2.2 reduced IHATS by 19% and this reduction was statistically significant only at 6 h after resupply of nitrate to nitrogen-deprived plants. Nitrates 133-140 nitrate transporter 2:1 Arabidopsis thaliana 24-30 16415212-6 2006 Nitrate induction of the nitrate transporter genes AtNRT1.1 and AtNRT2.1 was reduced in Atnrt3.1 mutant plants, and this reduced expression was correlated with reduced nitrate concentrations in the tissues. Nitrates 0-7 nitrate transporter 2:1 Arabidopsis thaliana 64-72 17012411-9 2006 Plants resulting from a cross between both mutants (atnrt2.1-1 x atnar2.1-1) showed a phenotype like that of the atnar2.1-1 mutant when grown in 0.5 mm nitrate. Nitrates 152-159 nitrate transporter 2:1 Arabidopsis thaliana 52-60 16415212-6 2006 Nitrate induction of the nitrate transporter genes AtNRT1.1 and AtNRT2.1 was reduced in Atnrt3.1 mutant plants, and this reduced expression was correlated with reduced nitrate concentrations in the tissues. Nitrates 25-32 nitrate transporter 2:1 Arabidopsis thaliana 64-72 10205909-1 1999 Putative high-affinity nitrate (NO3-) transporter genes, designated Nrt2;1At and Nrt2;2At, were isolated from Arabidopsis thaliana by RT-PCR using degenerate primers. Nitrates 23-30 nitrate transporter 2:1 Arabidopsis thaliana 68-72 16157886-8 2005 Furthermore, lateral root initiation is increased in lin1 relative to WT even when seedlings are grown on nitrate-free media, suggesting that the mutant phenotype is nitrate-independent. Nitrates 106-113 nitrate transporter 2:1 Arabidopsis thaliana 53-57 16157886-8 2005 Furthermore, lateral root initiation is increased in lin1 relative to WT even when seedlings are grown on nitrate-free media, suggesting that the mutant phenotype is nitrate-independent. Nitrates 166-173 nitrate transporter 2:1 Arabidopsis thaliana 53-57 16157886-10 2005 We propose that Arabidopsis NRT2.1 acts either as a nitrate sensor or signal transducer to coordinate the development of the root system with nutritional cues. Nitrates 52-59 nitrate transporter 2:1 Arabidopsis thaliana 28-32 12956537-5 2003 Experiments with various nitrogen supply regimes demonstrated the induction of NRT2.1 expression by nitrate and repression by amino acids. Nitrates 100-107 nitrate transporter 2:1 Arabidopsis thaliana 79-83 12956537-7 2003 Taken together, our results support the hypothesis that the NRT2.1 gene codes for a major component of the inducible high-affinity transport system for nitrate, which is spatially and developmentally controlled at the transcriptional level. Nitrates 152-159 nitrate transporter 2:1 Arabidopsis thaliana 60-66 12068127-4 2002 Using a reverse genetic approach, the AtNRT2.1 gene has been shown to be involved in the inducible component of the high-affinity nitrate transport system in Arabidopsis. Nitrates 130-137 nitrate transporter 2:1 Arabidopsis thaliana 38-46 11165253-0 2001 An arabidopsis T-DNA mutant affected in Nrt2 genes is impaired in nitrate uptake. Nitrates 66-73 nitrate transporter 2:1 Arabidopsis thaliana 40-44 11165253-1 2001 Expression analyses of Nrt2 plant genes have shown a strict correlation with root nitrate influx mediated by the high-affinity transport system (HATS). Nitrates 82-89 nitrate transporter 2:1 Arabidopsis thaliana 23-27 11165253-5 2001 Moreover, the de-regulated expression of a Nicotiana plumbaginifolia Nrt2 gene restored the mutant nitrate influx to that of the wild-type. Nitrates 99-106 nitrate transporter 2:1 Arabidopsis thaliana 69-73 16157886-5 2005 NRT2.1 encodes a putative high-affinity nitrate transporter that functions at low external nitrate concentrations. Nitrates 40-47 nitrate transporter 2:1 Arabidopsis thaliana 0-4 16157886-6 2005 Direct measurement of nitrate uptake and nitrate content in the lin1 mutant seedlings established that both are indeed reduced. Nitrates 22-29 nitrate transporter 2:1 Arabidopsis thaliana 64-68 16157886-6 2005 Direct measurement of nitrate uptake and nitrate content in the lin1 mutant seedlings established that both are indeed reduced. Nitrates 41-48 nitrate transporter 2:1 Arabidopsis thaliana 64-68 15107992-0 2004 Disruption of the nitrate transporter genes AtNRT2.1 and AtNRT2.2 restricts growth at low external nitrate concentration. Nitrates 18-25 nitrate transporter 2:1 Arabidopsis thaliana 44-52 15107992-0 2004 Disruption of the nitrate transporter genes AtNRT2.1 and AtNRT2.2 restricts growth at low external nitrate concentration. Nitrates 18-25 nitrate transporter 2:1 Arabidopsis thaliana 44-50 12956537-0 2003 Regulation of the nitrate transporter gene AtNRT2.1 in Arabidopsis thaliana: responses to nitrate, amino acids and developmental stage. Nitrates 18-25 nitrate transporter 2:1 Arabidopsis thaliana 43-51 12668777-0 2003 Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana: responses to nitrate provision. Nitrates 80-87 nitrate transporter 2:1 Arabidopsis thaliana 23-27 11912225-2 2002 Two classes of genes, NRT1 and NRT2, have been found to be potentially involved in the high and low affinity nitrate transport systems (HATS and LATS, respectively). Nitrates 109-116 nitrate transporter 2:1 Arabidopsis thaliana 31-35 11912225-3 2002 The complexity of the molecular basis of nitrate uptake has been enhanced by the finding that in many plants both NRT1 and NRT2 classes are represented by multigene families. Nitrates 41-48 nitrate transporter 2:1 Arabidopsis thaliana 123-127 11912225-5 2002 This is a review of recent progress in the characterization of the NRT2 nitrate transporters, the composition of this family in Arabidopsis, their possible role in nitrate acquisition, and some aspects of their regulation in plants. Nitrates 72-79 nitrate transporter 2:1 Arabidopsis thaliana 67-71 10205909-1 1999 Putative high-affinity nitrate (NO3-) transporter genes, designated Nrt2;1At and Nrt2;2At, were isolated from Arabidopsis thaliana by RT-PCR using degenerate primers. Nitrates 23-30 nitrate transporter 2:1 Arabidopsis thaliana 81-85 33329669-4 2020 Expression analyses of 35S::CDF3 lines reveled that CDF3 regulates the expression of an important set of nitrate responsive genes including, glutamine synthetase-1, glutamate synthase-2, nitrate reductase-1, and nitrate transporters NRT2.1, NRT2.4, and NRT2.5 as well as carbon assimilation genes like PK1 and PEPC1 in response to N availability. Nitrates 105-112 nitrate transporter 2:1 Arabidopsis thaliana 233-237 33758916-3 2021 Following N removal, plants activate their Nitrogen Starvation Response (NSR) being characterized in particular by the activation of very high-affinity nitrate transport systems (NRT2.4, NRT2.5) and other sentinel genes involved in N-remobilization such as GDH3. Nitrates 152-159 nitrate transporter 2:1 Arabidopsis thaliana 179-183 33758916-5 2021 First, HHOs directly repress the high-affinity nitrate transporters, NRT2.4 and NRT2.5. Nitrates 47-54 nitrate transporter 2:1 Arabidopsis thaliana 69-73 33868355-9 2021 Consistently, a loss-of-function mutant of a nitrate transporter gene NRT2.1 lacked low R:FR-induced lateral root reduction and its root growth was hypersensitive to low nitrate. Nitrates 45-52 nitrate transporter 2:1 Arabidopsis thaliana 70-74 33868355-14 2021 Together our results show that nitrate signaling can repress low R:FR responses and that this involves signaling via HY5 and NRT2.1. Nitrates 31-38 nitrate transporter 2:1 Arabidopsis thaliana 125-129 33686225-6 2021 Collectively, our results identify CEPH as a crucial enzyme in the N-starvation-dependent activation of NRT2.1 and provide molecular and mechanistic insights into how plants regulate high-affinity nitrate uptake at the post-translational level in response to the N environment. Nitrates 197-204 nitrate transporter 2:1 Arabidopsis thaliana 104-110 33643357-5 2021 An extended photoperiod also improved the root nitrate uptake of by NITRATE TRANSPORTER 1.1 (NRT1.1) and NITRATE TRANSPORTER 2.1 (NRT2.1), whereas the loss of function of NRT1.1/NRT2.1 in the nrt1.1-1/2.1-2 mutants suppressed the expression of the key flowering genes CONSTANS (CO) and FLOWERING LOCUS T (FT), and reduced the sensitivity of the photoperiodic flowering responses to elevated levels of nitrate. Nitrates 47-54 nitrate transporter 2:1 Arabidopsis thaliana 105-128 33643357-5 2021 An extended photoperiod also improved the root nitrate uptake of by NITRATE TRANSPORTER 1.1 (NRT1.1) and NITRATE TRANSPORTER 2.1 (NRT2.1), whereas the loss of function of NRT1.1/NRT2.1 in the nrt1.1-1/2.1-2 mutants suppressed the expression of the key flowering genes CONSTANS (CO) and FLOWERING LOCUS T (FT), and reduced the sensitivity of the photoperiodic flowering responses to elevated levels of nitrate. Nitrates 47-54 nitrate transporter 2:1 Arabidopsis thaliana 130-134 33643357-5 2021 An extended photoperiod also improved the root nitrate uptake of by NITRATE TRANSPORTER 1.1 (NRT1.1) and NITRATE TRANSPORTER 2.1 (NRT2.1), whereas the loss of function of NRT1.1/NRT2.1 in the nrt1.1-1/2.1-2 mutants suppressed the expression of the key flowering genes CONSTANS (CO) and FLOWERING LOCUS T (FT), and reduced the sensitivity of the photoperiodic flowering responses to elevated levels of nitrate. Nitrates 47-54 nitrate transporter 2:1 Arabidopsis thaliana 130-136 8799195-1 1996 Two mutations have been found in a gene (NRT2) of Arabidopsis thaliana that specifically impair constitutive, high-affinity nitrate uptake. Nitrates 124-131 nitrate transporter 2:1 Arabidopsis thaliana 41-45 8799195-7 1996 These results indicate that NRT2 is a critical and perhaps necessary gene for constitutive, high-affinity nitrate uptake in Arabidopsis, but not for inducible, high-affinity nor constitutive, low-affinity nitrate uptake. Nitrates 106-113 nitrate transporter 2:1 Arabidopsis thaliana 28-32 33686225-0 2021 A type 2C protein phosphatase activates high-affinity nitrate uptake by dephosphorylating NRT2.1. Nitrates 54-61 nitrate transporter 2:1 Arabidopsis thaliana 90-94 33686225-1 2021 The nitrate transporter NRT2.1, which plays a central role in high-affinity nitrate uptake in roots, is activated at the post-translational level in response to nitrogen (N) starvation1,2. Nitrates 4-11 nitrate transporter 2:1 Arabidopsis thaliana 24-28 33686225-3 2021 Here, we show that a type 2C protein phosphatase, designated CEPD-induced phosphatase (CEPH), activates high-affinity nitrate uptake by directly dephosphorylating Ser501 of NRT2.1, a residue that functions as a negative phospho-switch in Arabidopsis2. Nitrates 118-125 nitrate transporter 2:1 Arabidopsis thaliana 173-179 33582801-1 2021 In Arabidopsis (Arabidopsis thaliana), the High-Affinity Transport System (HATS) for root nitrate (NO3-) uptake depends mainly on four NRT2 NO3- transporters, namely NRT2.1, NRT2.2, NRT2.4, and NRT2.5. Nitrates 90-97 nitrate transporter 2:1 Arabidopsis thaliana 135-139 33582801-1 2021 In Arabidopsis (Arabidopsis thaliana), the High-Affinity Transport System (HATS) for root nitrate (NO3-) uptake depends mainly on four NRT2 NO3- transporters, namely NRT2.1, NRT2.2, NRT2.4, and NRT2.5. Nitrates 90-97 nitrate transporter 2:1 Arabidopsis thaliana 166-172 33582801-1 2021 In Arabidopsis (Arabidopsis thaliana), the High-Affinity Transport System (HATS) for root nitrate (NO3-) uptake depends mainly on four NRT2 NO3- transporters, namely NRT2.1, NRT2.2, NRT2.4, and NRT2.5. Nitrates 90-97 nitrate transporter 2:1 Arabidopsis thaliana 166-170 33582801-1 2021 In Arabidopsis (Arabidopsis thaliana), the High-Affinity Transport System (HATS) for root nitrate (NO3-) uptake depends mainly on four NRT2 NO3- transporters, namely NRT2.1, NRT2.2, NRT2.4, and NRT2.5. Nitrates 90-97 nitrate transporter 2:1 Arabidopsis thaliana 166-170 33582801-1 2021 In Arabidopsis (Arabidopsis thaliana), the High-Affinity Transport System (HATS) for root nitrate (NO3-) uptake depends mainly on four NRT2 NO3- transporters, namely NRT2.1, NRT2.2, NRT2.4, and NRT2.5. Nitrates 99-103 nitrate transporter 2:1 Arabidopsis thaliana 135-139 33582801-1 2021 In Arabidopsis (Arabidopsis thaliana), the High-Affinity Transport System (HATS) for root nitrate (NO3-) uptake depends mainly on four NRT2 NO3- transporters, namely NRT2.1, NRT2.2, NRT2.4, and NRT2.5. Nitrates 99-103 nitrate transporter 2:1 Arabidopsis thaliana 166-172 33582801-1 2021 In Arabidopsis (Arabidopsis thaliana), the High-Affinity Transport System (HATS) for root nitrate (NO3-) uptake depends mainly on four NRT2 NO3- transporters, namely NRT2.1, NRT2.2, NRT2.4, and NRT2.5. Nitrates 99-103 nitrate transporter 2:1 Arabidopsis thaliana 166-170 33582801-1 2021 In Arabidopsis (Arabidopsis thaliana), the High-Affinity Transport System (HATS) for root nitrate (NO3-) uptake depends mainly on four NRT2 NO3- transporters, namely NRT2.1, NRT2.2, NRT2.4, and NRT2.5. Nitrates 99-103 nitrate transporter 2:1 Arabidopsis thaliana 166-170 33329669-4 2020 Expression analyses of 35S::CDF3 lines reveled that CDF3 regulates the expression of an important set of nitrate responsive genes including, glutamine synthetase-1, glutamate synthase-2, nitrate reductase-1, and nitrate transporters NRT2.1, NRT2.4, and NRT2.5 as well as carbon assimilation genes like PK1 and PEPC1 in response to N availability. Nitrates 105-112 nitrate transporter 2:1 Arabidopsis thaliana 241-245 33329669-4 2020 Expression analyses of 35S::CDF3 lines reveled that CDF3 regulates the expression of an important set of nitrate responsive genes including, glutamine synthetase-1, glutamate synthase-2, nitrate reductase-1, and nitrate transporters NRT2.1, NRT2.4, and NRT2.5 as well as carbon assimilation genes like PK1 and PEPC1 in response to N availability. Nitrates 105-112 nitrate transporter 2:1 Arabidopsis thaliana 241-245 31342638-0 2020 Phosphorylation at Ser28 stabilizes the Arabidopsis nitrate transporter NRT2.1 in response to nitrate limitation. Nitrates 52-59 nitrate transporter 2:1 Arabidopsis thaliana 72-78 32463943-0 2020 NRT2.1 C-terminus phosphorylation prevents root high affinity nitrate uptake activity in Arabidopsis thaliana. Nitrates 62-69 nitrate transporter 2:1 Arabidopsis thaliana 0-6 32463943-1 2020 In Arabidopsis thaliana, NRT2.1 codes for a main component of the root nitrate high-affinity transport system. Nitrates 71-78 nitrate transporter 2:1 Arabidopsis thaliana 25-29 32463943-2 2020 Previous studies revealed that post-translational regulation of NRT2.1 plays an important role in the control of root nitrate uptake and that one mechanism could correspond to NRT2.1 C-terminus processing. Nitrates 118-125 nitrate transporter 2:1 Arabidopsis thaliana 64-70 32463943-2 2020 Previous studies revealed that post-translational regulation of NRT2.1 plays an important role in the control of root nitrate uptake and that one mechanism could correspond to NRT2.1 C-terminus processing. Nitrates 118-125 nitrate transporter 2:1 Arabidopsis thaliana 64-68 32463943-7 2020 Finally, the relative level of serine 501 phosphorylation was found to be increased by ammonium nitrate in wild-type plants, leading to the inactivation of NRT2.1 and to a decrease in high affinity nitrate transport into roots. Nitrates 96-103 nitrate transporter 2:1 Arabidopsis thaliana 156-162 33036562-1 2020 BACKGROUND: High-affinity nitrate transporter 2 (NRT2) genes have been implicated in nitrate absorption and remobilization under nitrogen (N) starvation stress in many plant species, yet little is known about this gene family respond to various stresses often occurs in the production of rapeseed (Brassica napus L.). Nitrates 26-33 nitrate transporter 2:1 Arabidopsis thaliana 49-53 32526869-2 2020 One of the essential nutrients, nitrate (NO3-), is sensed and transported by nitrate transporters NRT1.1 and NRT2.1 in the plants. Nitrates 32-39 nitrate transporter 2:1 Arabidopsis thaliana 109-113 32526869-2 2020 One of the essential nutrients, nitrate (NO3-), is sensed and transported by nitrate transporters NRT1.1 and NRT2.1 in the plants. Nitrates 41-45 nitrate transporter 2:1 Arabidopsis thaliana 109-113 32526869-2 2020 One of the essential nutrients, nitrate (NO3-), is sensed and transported by nitrate transporters NRT1.1 and NRT2.1 in the plants. Nitrates 77-84 nitrate transporter 2:1 Arabidopsis thaliana 109-113 31342638-9 2020 These results demonstrate that phosphorylation at Ser28 is crucial for NRT2.1 protein stabilization and accumulation in response to nitrate limitation. Nitrates 132-139 nitrate transporter 2:1 Arabidopsis thaliana 71-77 31342638-3 2020 Nitrate transporter 2.1 (NRT2.1) is the major component of the root high-affinity nitrate transport system in Arabidopsis thaliana. Nitrates 82-89 nitrate transporter 2:1 Arabidopsis thaliana 0-23 31342638-3 2020 Nitrate transporter 2.1 (NRT2.1) is the major component of the root high-affinity nitrate transport system in Arabidopsis thaliana. Nitrates 82-89 nitrate transporter 2:1 Arabidopsis thaliana 25-29 31342638-6 2020 When seedlings were transferred to nitrate-limited conditions, the apparent half-life of NRT2.1 in roots increased from 3 to 9 h. This stabilization of NRT2.1 protein occurred rapidly, even prior to the transcriptional stimulation of NRT2.1. Nitrates 35-42 nitrate transporter 2:1 Arabidopsis thaliana 89-95 31342638-6 2020 When seedlings were transferred to nitrate-limited conditions, the apparent half-life of NRT2.1 in roots increased from 3 to 9 h. This stabilization of NRT2.1 protein occurred rapidly, even prior to the transcriptional stimulation of NRT2.1. Nitrates 35-42 nitrate transporter 2:1 Arabidopsis thaliana 152-158 31342638-6 2020 When seedlings were transferred to nitrate-limited conditions, the apparent half-life of NRT2.1 in roots increased from 3 to 9 h. This stabilization of NRT2.1 protein occurred rapidly, even prior to the transcriptional stimulation of NRT2.1. Nitrates 35-42 nitrate transporter 2:1 Arabidopsis thaliana 152-158 29541882-5 2018 Quantitative reverse transcription polymerase chain reaction analysis indicated high expression levels of the nitrate transporter genes, especially NRT2.1, which plays a major role in the high-affinity nitrate transport system in roots. Nitrates 110-117 nitrate transporter 2:1 Arabidopsis thaliana 148-154 31172512-2 2019 Here, we show that the induction of NRT2.1- and NRT2.2-mediated nitrate uptake interferes with measurements of the contribution of NRT1.1 to high-affinity uptake using nrt1.1 mutants. Nitrates 64-71 nitrate transporter 2:1 Arabidopsis thaliana 36-40 31172512-2 2019 Here, we show that the induction of NRT2.1- and NRT2.2-mediated nitrate uptake interferes with measurements of the contribution of NRT1.1 to high-affinity uptake using nrt1.1 mutants. Nitrates 64-71 nitrate transporter 2:1 Arabidopsis thaliana 48-52 31172512-4 2019 This triple mutant has a lower rate of nitrate uptake than the nrt2.1/2.2 double mutant under low external nitrate supply, resulting in a lower growth rate than that of the double mutant. Nitrates 39-46 nitrate transporter 2:1 Arabidopsis thaliana 63-67 31172512-4 2019 This triple mutant has a lower rate of nitrate uptake than the nrt2.1/2.2 double mutant under low external nitrate supply, resulting in a lower growth rate than that of the double mutant. Nitrates 107-114 nitrate transporter 2:1 Arabidopsis thaliana 63-67 31915951-5 2020 Quantitative reverse transcription polymerase chain reaction analysis revealed a high level of expression of the high affinity nitrate transporter gene, NRT2.1 in A. thaliana treated with Bs L1. Nitrates 127-134 nitrate transporter 2:1 Arabidopsis thaliana 153-157 30042774-5 2018 It has also been identified as a nitrate sensor which regulates the expression of high-affinity nitrate transport proteins NRT2s and lateral root development as a part of the primary nitrate response in plants. Nitrates 33-40 nitrate transporter 2:1 Arabidopsis thaliana 123-127 30042774-5 2018 It has also been identified as a nitrate sensor which regulates the expression of high-affinity nitrate transport proteins NRT2s and lateral root development as a part of the primary nitrate response in plants. Nitrates 96-103 nitrate transporter 2:1 Arabidopsis thaliana 123-127 29636481-4 2018 Here we identify NIGT1 transcriptional repressors as negative regulators of the Arabidopsis NRT2.1 nitrate transporter gene, and show antagonistic regulation by NLP primary transcription factors for nitrate signalling and the NLP-NIGT1 transcriptional cascade-mediated repression. Nitrates 99-106 nitrate transporter 2:1 Arabidopsis thaliana 92-98 29636481-6 2018 Genome-wide analysis reveals that this mechanism is applicable to NRT2.1 and other genes involved in nitrate assimilation, hormone biosynthesis and transcription. Nitrates 101-108 nitrate transporter 2:1 Arabidopsis thaliana 66-72 27004464-1 2016 The family of NITRATE TRANSPORTER 2 (NRT2) proteins belongs to the high affinity transport system (HATS) proteins which acts at low nitrate concentrations. Nitrates 132-139 nitrate transporter 2:1 Arabidopsis thaliana 14-35 28319056-6 2017 These shoot-derived polypeptides, which we named CEP DOWNSTREAM 1 (CEPD1) and CEPD2, upregulate the expression of the nitrate transporter gene NRT2.1 in roots specifically when nitrate is present in the rhizosphere. Nitrates 118-125 nitrate transporter 2:1 Arabidopsis thaliana 143-149 27004464-1 2016 The family of NITRATE TRANSPORTER 2 (NRT2) proteins belongs to the high affinity transport system (HATS) proteins which acts at low nitrate concentrations. Nitrates 132-139 nitrate transporter 2:1 Arabidopsis thaliana 37-41 27246882-4 2015 We show that the point mutations differentially affect several of the NRT1.1-dependent responses to nitrate, namely the repression of lateral root development at low nitrate concentrations, and the short-term upregulation of the nitrate-uptake gene NRT2.1, and its longer-term downregulation, at high nitrate concentrations. Nitrates 100-107 nitrate transporter 2:1 Arabidopsis thaliana 249-255 26877080-4 2016 Shoot-derived HY5 auto-activates root HY5 and also promotes root nitrate uptake by activating NRT2.1, a gene encoding a high-affinity nitrate transporter [4]. Nitrates 65-72 nitrate transporter 2:1 Arabidopsis thaliana 94-100 25474587-6 2015 The remaining cHATS nitrate influx in these mutants is due to a residual contribution by the inducible high-affinity transporter encoded by AtNRT2.1/AtNAR2.1. Nitrates 20-27 nitrate transporter 2:1 Arabidopsis thaliana 140-148 25474587-7 2015 Estimates of the kinetic properties of the NRT2.5 transporter reveal that its low Km for nitrate makes this transporter ideally suited to detect and respond to trace quantities of nitrate in the root environment. Nitrates 89-96 nitrate transporter 2:1 Arabidopsis thaliana 43-47 25474587-7 2015 Estimates of the kinetic properties of the NRT2.5 transporter reveal that its low Km for nitrate makes this transporter ideally suited to detect and respond to trace quantities of nitrate in the root environment. Nitrates 180-187 nitrate transporter 2:1 Arabidopsis thaliana 43-47 27246882-4 2015 We show that the point mutations differentially affect several of the NRT1.1-dependent responses to nitrate, namely the repression of lateral root development at low nitrate concentrations, and the short-term upregulation of the nitrate-uptake gene NRT2.1, and its longer-term downregulation, at high nitrate concentrations. Nitrates 166-173 nitrate transporter 2:1 Arabidopsis thaliana 249-255 27246882-4 2015 We show that the point mutations differentially affect several of the NRT1.1-dependent responses to nitrate, namely the repression of lateral root development at low nitrate concentrations, and the short-term upregulation of the nitrate-uptake gene NRT2.1, and its longer-term downregulation, at high nitrate concentrations. Nitrates 166-173 nitrate transporter 2:1 Arabidopsis thaliana 249-255 27246882-4 2015 We show that the point mutations differentially affect several of the NRT1.1-dependent responses to nitrate, namely the repression of lateral root development at low nitrate concentrations, and the short-term upregulation of the nitrate-uptake gene NRT2.1, and its longer-term downregulation, at high nitrate concentrations. Nitrates 166-173 nitrate transporter 2:1 Arabidopsis thaliana 249-255 23305042-0 2013 The nitrate transporter NRT2.1 functions in the ethylene response to nitrate deficiency in Arabidopsis. Nitrates 4-11 nitrate transporter 2:1 Arabidopsis thaliana 24-30 25596127-6 2015 Expressions of the high-affinity nitrate transporter genes NRT2.1 and NRT2.2 and nitrate reductase genes NIA1 and NIA2 in transgenic plants were all higher than in wild type indicating that higher levels of nitrate transport and assimilation activity contributed to enhanced drought resistance of AtTGA4 transgenic plants. Nitrates 33-40 nitrate transporter 2:1 Arabidopsis thaliana 59-65 25065551-0 2014 The Arabidopsis nitrate transporter NRT2.5 plays a role in nitrate acquisition and remobilization in nitrogen-starved plants. Nitrates 16-23 nitrate transporter 2:1 Arabidopsis thaliana 36-40 25065551-6 2014 Reduction of NRT2.5 expression resulted in a decrease in high-affinity nitrate uptake without impacting low-affinity uptake. Nitrates 71-78 nitrate transporter 2:1 Arabidopsis thaliana 13-17 25065551-7 2014 In the background of the high-affinity nitrate transporter mutant nrt2.4, an nrt2.5 mutation reduced nitrate levels in the phloem of N-starved plants further than in the single nrt2.4 mutants. Nitrates 39-46 nitrate transporter 2:1 Arabidopsis thaliana 66-70 25065551-7 2014 In the background of the high-affinity nitrate transporter mutant nrt2.4, an nrt2.5 mutation reduced nitrate levels in the phloem of N-starved plants further than in the single nrt2.4 mutants. Nitrates 39-46 nitrate transporter 2:1 Arabidopsis thaliana 77-81 25065551-7 2014 In the background of the high-affinity nitrate transporter mutant nrt2.4, an nrt2.5 mutation reduced nitrate levels in the phloem of N-starved plants further than in the single nrt2.4 mutants. Nitrates 39-46 nitrate transporter 2:1 Arabidopsis thaliana 77-81 24272701-6 2014 Nitrate-mediated NRT2.1 expression is not influenced by gin2-1, showing that Glc does not influence NRT2.1 expression through nitrate-mediated mechanisms. Nitrates 0-7 nitrate transporter 2:1 Arabidopsis thaliana 17-23 24272701-7 2014 We also show that Glc stimulates NRT2.1 protein levels and transport activity independently of its HEXOKINASE1-mediated stimulation of NRT2.1 expression, demonstrating another possible posttranscriptional mechanism influencing nitrate uptake. Nitrates 227-234 nitrate transporter 2:1 Arabidopsis thaliana 33-39 24532452-1 2014 NRT2.7 is a seed-specific high-affinity nitrate transporter controlling nitrate content in Arabidopsis mature seeds. Nitrates 40-47 nitrate transporter 2:1 Arabidopsis thaliana 0-4 23305042-3 2013 However, molecular interaction between NRT2.1 transcript levels and the ethylene signalling pathway under nitrate deficiency is still elusive. Nitrates 106-113 nitrate transporter 2:1 Arabidopsis thaliana 39-45 23305042-5 2013 LN treatment also caused up-regulation of NRT2.1 expression, which was responsible for an enhanced high-affinity nitrate uptake. Nitrates 113-120 nitrate transporter 2:1 Arabidopsis thaliana 42-48 23305042-8 2013 Together, these findings uncover a negative feedback loop between NRT2.1 expression and ethylene biosynthesis and signalling under nitrate deficiency, which may contribute to finely tuning of plant nitrate acquisition during exploring dynamic soil conditions. Nitrates 131-138 nitrate transporter 2:1 Arabidopsis thaliana 66-72 23305042-8 2013 Together, these findings uncover a negative feedback loop between NRT2.1 expression and ethylene biosynthesis and signalling under nitrate deficiency, which may contribute to finely tuning of plant nitrate acquisition during exploring dynamic soil conditions. Nitrates 198-205 nitrate transporter 2:1 Arabidopsis thaliana 66-72 23305042-2 2013 Previous studies have reported impact of high nitrate (HN) availability on ethylene biosynthesis and regulation of ethylene on nitrate transporter 2.1 (NRT2.1) expression. Nitrates 46-53 nitrate transporter 2:1 Arabidopsis thaliana 127-150 23305042-2 2013 Previous studies have reported impact of high nitrate (HN) availability on ethylene biosynthesis and regulation of ethylene on nitrate transporter 2.1 (NRT2.1) expression. Nitrates 46-53 nitrate transporter 2:1 Arabidopsis thaliana 152-158 23398541-8 2013 Using a nitrate reductase mutant, it was confirmed that the NRT2.5/NRT2.6-dependent plant signalling pathway is independent of nitrate-dependent regulation of root development. Nitrates 8-15 nitrate transporter 2:1 Arabidopsis thaliana 60-64 23398541-8 2013 Using a nitrate reductase mutant, it was confirmed that the NRT2.5/NRT2.6-dependent plant signalling pathway is independent of nitrate-dependent regulation of root development. Nitrates 8-15 nitrate transporter 2:1 Arabidopsis thaliana 67-71 21486304-3 2011 A two-component system for nitrate transport including NRT2s with a partner protein (NAR2 or NRT3.1) has been identified in Arabidopsis. Nitrates 27-34 nitrate transporter 2:1 Arabidopsis thaliana 55-59 22432443-0 2012 Nitrate transport capacity of the Arabidopsis thaliana NRT2 family members and their interactions with AtNAR2.1. Nitrates 0-7 nitrate transporter 2:1 Arabidopsis thaliana 55-59 22432443-1 2012 Interactions between the Arabidopsis NitRate Transporter (AtNRT2.1) and Nitrate Assimilation Related protein (AtNAR2.1, also known as AtNRT3.1) have been well documented, and confirmed by the demonstration that AtNRT2.1 and AtNAR2.1 form a 150-kDa plasma membrane complex, thought to constitute the high-affinity nitrate transporter of Arabidopsis thaliana roots. Nitrates 72-79 nitrate transporter 2:1 Arabidopsis thaliana 58-66 22432443-1 2012 Interactions between the Arabidopsis NitRate Transporter (AtNRT2.1) and Nitrate Assimilation Related protein (AtNAR2.1, also known as AtNRT3.1) have been well documented, and confirmed by the demonstration that AtNRT2.1 and AtNAR2.1 form a 150-kDa plasma membrane complex, thought to constitute the high-affinity nitrate transporter of Arabidopsis thaliana roots. Nitrates 72-79 nitrate transporter 2:1 Arabidopsis thaliana 211-219 22158677-0 2012 Regulation of high-affinity nitrate uptake in roots of Arabidopsis depends predominantly on posttranscriptional control of the NRT2.1/NAR2.1 transport system. Nitrates 28-35 nitrate transporter 2:1 Arabidopsis thaliana 127-133 22158677-1 2012 In Arabidopsis (Arabidopsis thaliana), the NRT2.1 gene codes for the main component of the root nitrate (NO(3)(-)) high-affinity transport system (HATS). Nitrates 96-103 nitrate transporter 2:1 Arabidopsis thaliana 43-49 22158760-2 2012 Nitrate Trasnporter2 (NRT2) gene family members are sentinels of nitrate availability. Nitrates 0-7 nitrate transporter 2:1 Arabidopsis thaliana 22-26 22158760-2 2012 Nitrate Trasnporter2 (NRT2) gene family members are sentinels of nitrate availability. Nitrates 65-72 nitrate transporter 2:1 Arabidopsis thaliana 22-26 22227893-6 2012 In N-starved nrt2.4 mutants, nitrate uptake under low external supply and nitrate content in shoot phloem exudates was decreased. Nitrates 29-36 nitrate transporter 2:1 Arabidopsis thaliana 13-17 22227893-6 2012 In N-starved nrt2.4 mutants, nitrate uptake under low external supply and nitrate content in shoot phloem exudates was decreased. Nitrates 74-81 nitrate transporter 2:1 Arabidopsis thaliana 13-17 22880003-1 2012 The high affinity nitrate transport system in Arabidopsis thaliana involves one gene and potentially seven genes from the NRT1 and NRT2 family, respectively. Nitrates 18-25 nitrate transporter 2:1 Arabidopsis thaliana 131-135 22880003-2 2012 Among them, NRT2.1, NRT2.2, NRT2.4 and NRT2.7 proteins have been shown to transport nitrate and are localized on the plasmalemma or the tonoplast membranes. Nitrates 84-91 nitrate transporter 2:1 Arabidopsis thaliana 12-18 22880003-2 2012 Among them, NRT2.1, NRT2.2, NRT2.4 and NRT2.7 proteins have been shown to transport nitrate and are localized on the plasmalemma or the tonoplast membranes. Nitrates 84-91 nitrate transporter 2:1 Arabidopsis thaliana 12-16 22880003-2 2012 Among them, NRT2.1, NRT2.2, NRT2.4 and NRT2.7 proteins have been shown to transport nitrate and are localized on the plasmalemma or the tonoplast membranes. Nitrates 84-91 nitrate transporter 2:1 Arabidopsis thaliana 20-24 22880003-2 2012 Among them, NRT2.1, NRT2.2, NRT2.4 and NRT2.7 proteins have been shown to transport nitrate and are localized on the plasmalemma or the tonoplast membranes. Nitrates 84-91 nitrate transporter 2:1 Arabidopsis thaliana 20-24 22880003-3 2012 NRT2.1, NRT2.2 and NRT2.4 play a role in nitrate uptake from soil solution by root cells while NRT2.7 is responsible for nitrate loading in the seed vacuole. Nitrates 41-48 nitrate transporter 2:1 Arabidopsis thaliana 0-6 22880003-3 2012 NRT2.1, NRT2.2 and NRT2.4 play a role in nitrate uptake from soil solution by root cells while NRT2.7 is responsible for nitrate loading in the seed vacuole. Nitrates 41-48 nitrate transporter 2:1 Arabidopsis thaliana 0-4 22880003-3 2012 NRT2.1, NRT2.2 and NRT2.4 play a role in nitrate uptake from soil solution by root cells while NRT2.7 is responsible for nitrate loading in the seed vacuole. Nitrates 41-48 nitrate transporter 2:1 Arabidopsis thaliana 8-12 22880003-3 2012 NRT2.1, NRT2.2 and NRT2.4 play a role in nitrate uptake from soil solution by root cells while NRT2.7 is responsible for nitrate loading in the seed vacuole. Nitrates 41-48 nitrate transporter 2:1 Arabidopsis thaliana 8-12 22880003-3 2012 NRT2.1, NRT2.2 and NRT2.4 play a role in nitrate uptake from soil solution by root cells while NRT2.7 is responsible for nitrate loading in the seed vacuole. Nitrates 121-128 nitrate transporter 2:1 Arabidopsis thaliana 0-6 22880003-3 2012 NRT2.1, NRT2.2 and NRT2.4 play a role in nitrate uptake from soil solution by root cells while NRT2.7 is responsible for nitrate loading in the seed vacuole. Nitrates 121-128 nitrate transporter 2:1 Arabidopsis thaliana 0-4