PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 12894505-0 2003 The antitumor ether lipid 1-Q-octadecyl-2-O-methyl-rac-glycerophosphocholine (ET-18-OCH3) inhibits the association between Ras and Raf-1. edelfosine 78-88 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 131-136 12894505-4 2003 Since ET-18-OCH3 did not inhibit the activities of the kinases in the cascade, the reduced Raf-1 levels appeared to be the cause for the reduction in the magnitude and duration of MAPK activity. edelfosine 6-16 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 91-96 12894505-12 2003 CONCLUSION: Our findings suggest that ET-18-OCH3 associates specifically with Raf-1 in the cytosol and interferes in the interaction of Raf-1 with activated Ras, thereby reducing the levels that are translocated to the membrane for activation. edelfosine 38-48 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 78-83 12894505-12 2003 CONCLUSION: Our findings suggest that ET-18-OCH3 associates specifically with Raf-1 in the cytosol and interferes in the interaction of Raf-1 with activated Ras, thereby reducing the levels that are translocated to the membrane for activation. edelfosine 38-48 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 136-141 12894505-13 2003 Thus Raf-1 appears to be a molecular target of ET-18-OCH3. edelfosine 47-57 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 5-10 10519389-0 1999 Decreased sensitivity to 1-O-octadecyl-2-O-methyl-glycerophosphocholine in MCF-7 cells adapted for serum-free growth correlates with constitutive association of Raf-1 with cellular membranes. edelfosine 25-71 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 161-166 10519389-1 1999 We have previously shown that inhibition of MCF-7 cell proliferation by 1-O-octadecyl-2-O-methyl-glycerophosphocholine (ET-18-OCH3) is linked to a drug-induced decrease in membrane Raf-1 levels and the subsequent inhibition of mitogen-activated protein (MAP) kinase activation in response to growth factor stimulation. edelfosine 72-118 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 181-186 10519389-6 1999 In both cell types, incubation with ET-18-OCH3 had no effect on the membrane-Raf-1 levels, suggesting that ET-18-OCH3-induced reduction of Raf-1 levels in growth factor-stimulated MCF-7 cells is due to inhibition of Raf translocation. edelfosine 107-117 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 139-144 8770865-6 1996 However, an interference in the association of Raf-1 with membranes and a resultant decrease in Raf-1 kinase activity in membranes of ET18-OCH3-treated cells was observed. edelfosine 134-143 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 47-52 8770865-6 1996 However, an interference in the association of Raf-1 with membranes and a resultant decrease in Raf-1 kinase activity in membranes of ET18-OCH3-treated cells was observed. edelfosine 134-143 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 96-101 8770865-9 1996 These results suggest that inhibition of the MAPK cascade by ET18-OCH3 as a result of its effect on Raf-1 activation may be an important mechanism by which ET18-OCH3 inhibits cell proliferation. edelfosine 61-70 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 100-105 8770865-9 1996 These results suggest that inhibition of the MAPK cascade by ET18-OCH3 as a result of its effect on Raf-1 activation may be an important mechanism by which ET18-OCH3 inhibits cell proliferation. edelfosine 156-165 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 100-105