PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 31715251-6 2020 After the evaluation of 19 different combination, the best condition to express HBD2 had a pre-induction temperature of 37 C, a cell density of 1.0 U (600 nm), an induction temperature of 20 C and a 0.1 mM of gene expression inducer (IPTG) over four hours. Isopropyl Thiogalactoside 236-240 defensin beta 4A Homo sapiens 80-84 16322988-5 2006 Under the optimized conditions of cultivation and isopropylthiogalactoside (IPTG) induction, the maximum expression level of soluble mature hBD2 (0.76 g/l) with the highest percentage of fusion protein in soluble proteins (62.2%) was obtained in the present work, which was the highest yield of hBD2 reported so far. Isopropyl Thiogalactoside 50-74 defensin beta 4A Homo sapiens 140-144 16322988-5 2006 Under the optimized conditions of cultivation and isopropylthiogalactoside (IPTG) induction, the maximum expression level of soluble mature hBD2 (0.76 g/l) with the highest percentage of fusion protein in soluble proteins (62.2%) was obtained in the present work, which was the highest yield of hBD2 reported so far. Isopropyl Thiogalactoside 76-80 defensin beta 4A Homo sapiens 140-144