PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 16755929-7 2006 IL-1ra and its mutants were expressed in E. coli BL21 (DE3) using pTIG-Trx expressing system with the induction of IPTG. Isopropyl Thiogalactoside 115-119 thioredoxin Homo sapiens 71-74 19256346-4 2008 The soluble fusion protein Trx-KDR3 was expressed in cytoplasm, which was up to 20% of total soluble protein in cytoplasm after having been induced by 1 mmol/L IPTG for 5 h at 30 degrees C. It was characterized to be target protein by Western blotting. Isopropyl Thiogalactoside 160-164 thioredoxin Homo sapiens 27-30 18562210-2 2008 Transformation of BL21 cells and induction with IPTG results in the high level expression of the protein fused N-terminally with thioredoxin and bearing a His-tag. Isopropyl Thiogalactoside 48-52 thioredoxin Homo sapiens 129-140 24814641-4 2014 In the E. coli expression system, a large amount of soluble thioredoxin (Trx)-hCTRP1 fusion protein could be produced using the expression plasmid pET32a (+) and induction with IPTG at 18 C, which accounts about 20% of the total soluble bacterial proteins. Isopropyl Thiogalactoside 177-181 thioredoxin Homo sapiens 73-76