PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 1658333-2 1991 To evaluate the biological relevance of the thioester adduct between RAD6 protein and ubiquitin, formed as an obligatory, transient intermediate during ubiquitin conjugation to substrates, we altered cysteine 88 in RAD6 to serine. Serine 223-229 E2 ubiquitin-conjugating protein RAD6 Saccharomyces cerevisiae S288C 69-73 1658333-2 1991 To evaluate the biological relevance of the thioester adduct between RAD6 protein and ubiquitin, formed as an obligatory, transient intermediate during ubiquitin conjugation to substrates, we altered cysteine 88 in RAD6 to serine. Serine 223-229 E2 ubiquitin-conjugating protein RAD6 Saccharomyces cerevisiae S288C 215-219