PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 30013652-10 2018 In addition, phosphorylation and subsequent activation of protein kinase B (Akt), which is known to phosphorylate GSK-3beta at serine 9 (Ser9), leading to its inhibition, were significantly decreased by juglone in LNCaP and LNCaP-AI cells. Serine 127-133 protein tyrosine kinase 2 beta Homo sapiens 58-74 26925779-6 2016 In particular, a functionally critical STK-conserved histidine that stabilizes the regulatory spine in STKs is selectively mutated to an alanine, serine or glutamate in PTKs, and this loss-of-function mutation is accommodated, in part, through compensatory PTK-specific interactions in the core. Serine 146-152 protein tyrosine kinase 2 beta Homo sapiens 169-172 27067543-1 2016 Non-canonical transforming growth factor beta (TGFbeta) signaling through protein kinase B (Akt2) induces phosphorylation of heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1) at serine-43 (p-hnRNP E1). Serine 182-188 protein tyrosine kinase 2 beta Homo sapiens 74-90 22707636-5 2012 In addition, TNF-alpha treatment inhibited glycogen synthase kinase 3beta (GSK-3beta) activity through serine-9 phosphorylation mediated by the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway in RCC cells. Serine 103-109 protein tyrosine kinase 2 beta Homo sapiens 174-190 25882230-8 2015 It also suppressed NF-kappaB translocated to the nucleus and inhibited cAMP response element-binding protein (CREB) Ser-133 phosphorylation level in the phosphoinositide 3-kinase/protein kinase B/CREB (PI3K/AKT/CREB) pathway. Serine 116-119 protein tyrosine kinase 2 beta Homo sapiens 179-195 25979114-5 2015 Emerging evidence suggests that two key post-translational modifications of polyglutamine-expanded androgen receptor, namely serine phosphorylation by protein kinase B/Akt and arginine methylation by protein arginine methyltransferases, occur at the same consensus site, are mutually exclusive, and have opposing effects on neurotoxicity. Serine 125-131 protein tyrosine kinase 2 beta Homo sapiens 151-167 23690212-5 2013 Taken together, TPA could degrade PKB via the ubiquitin-proteasomal pathway, and the suppression of PKB phosphorylation at the serine 473 site might be a prerequisite for the TPA-induced ubiquitination in gastric cancer cells. Serine 127-133 protein tyrosine kinase 2 beta Homo sapiens 100-103 23139742-8 2012 Bioinformatics results identified 307(Thr) of FSHR to be a site for O-linked glycosylation, 680(Ser) of FSHR a phosphorylated site by protein kinase B, and 289(Ser) of HSD17B3 a phosphorylated site by protein kinase B or ribosomal protein S6 kinase 1. Serine 96-99 protein tyrosine kinase 2 beta Homo sapiens 134-150 19581418-6 2009 The IRS-1 protein expression was reduced and the serine phosphorylation of PKB in response to insulin attenuated whereas basal and insulin-stimulated phosphorylation of extracellular signal-related kinase (ERK)1/2 was increased in type 2 diabetes MVEC. Serine 49-55 protein tyrosine kinase 2 beta Homo sapiens 75-78 20513353-4 2010 Here we demonstrate that purified PIKfyve is phosphorylated in vitro by a recombinant active PKB on two separate serine residues, S318 and S105, which flank the N-terminal FYVE domain of the protein. Serine 113-119 protein tyrosine kinase 2 beta Homo sapiens 93-96 20513353-8 2010 Our results confirm, therefore, that PIKfyve is directly phosphorylated by PKB on a single serine residue in response to insulin and are not due to autophosphorylation of the enzyme. Serine 91-97 protein tyrosine kinase 2 beta Homo sapiens 75-78 20534540-5 2010 We demonstrate that this effect is mediated by activation of the serine/threonine kinase, protein kinase B, which inhibits AMPK by phosphorylating serine 485. Serine 65-71 protein tyrosine kinase 2 beta Homo sapiens 90-106 20515848-3 2010 The Akt / protein kinase B (PKB) is a family of serine / threonine protein kinases, which play prominent roles in a diverse number of processes including cell survival, cell growth, gene expression, apoptosis, protein synthesis, energy metabolism and oncogenesis. Serine 48-54 protein tyrosine kinase 2 beta Homo sapiens 10-26 20515848-3 2010 The Akt / protein kinase B (PKB) is a family of serine / threonine protein kinases, which play prominent roles in a diverse number of processes including cell survival, cell growth, gene expression, apoptosis, protein synthesis, energy metabolism and oncogenesis. Serine 48-54 protein tyrosine kinase 2 beta Homo sapiens 28-31 21592956-0 2011 mTORC2 protein complex-mediated Akt (Protein Kinase B) Serine 473 Phosphorylation is not required for Akt1 activity in human platelets [corrected]. Serine 55-61 protein tyrosine kinase 2 beta Homo sapiens 37-53 21592956-1 2011 Protein kinase B (PKB, Akt) is a Ser/Thr kinase involved in the regulation of cell survival, proliferation, and metabolism and is activated by dual phosphorylation on Thr(308) in the activation loop and Ser(473) in the hydrophobic motif. Serine 33-36 protein tyrosine kinase 2 beta Homo sapiens 0-16 21592956-1 2011 Protein kinase B (PKB, Akt) is a Ser/Thr kinase involved in the regulation of cell survival, proliferation, and metabolism and is activated by dual phosphorylation on Thr(308) in the activation loop and Ser(473) in the hydrophobic motif. Serine 33-36 protein tyrosine kinase 2 beta Homo sapiens 18-21 21195757-7 2011 Additionally, Pyk2 recovered with the NT antibody displays increased serine/threonine phosphorylation. Serine 69-75 protein tyrosine kinase 2 beta Homo sapiens 14-18 21195757-9 2011 Upon induction of serine and/or threonine phosphorylation of Pyk2, it opens to a confirmation that allows for antibody binding to the NT epitope but at the same time no longer binds paxillin or the CT antiserum. Serine 18-24 protein tyrosine kinase 2 beta Homo sapiens 61-65 18718528-0 2008 Serine 396 of PDK1 is required for maximal PKB activation. Serine 0-6 protein tyrosine kinase 2 beta Homo sapiens 43-46 19584241-7 2009 Moreover, the antiapoptotic protein Akt/protein kinase B was down-regulated and also dephosphorylated at residue Ser(473) in a dose- and time-dependent manner and NB cells entered apoptotic cell death. Serine 113-116 protein tyrosine kinase 2 beta Homo sapiens 40-56 19144640-2 2009 A recent study showed that DNA-PK is responsible for Ser-473 phosphorylation in the hydrophobic motif of protein kinase B (PKB/Akt) in genotoxic-stressed cells, suggesting a novel role for DNA-PK in cell signaling. Serine 53-56 protein tyrosine kinase 2 beta Homo sapiens 105-121 18667411-6 2008 Comparison across tissue specimens showed an >20% decrease of protein kinase B (AKT) Ser-473 (p < 0.002) and myristoylated alanine-rich C-kinase substrate protein Ser-152/156 (p < 0.0001) within the first 90-min postexcision. Serine 88-91 protein tyrosine kinase 2 beta Homo sapiens 65-81 18687633-8 2008 These data suggest that a higher proportion of the EGFR mutant carcinoma cells may exhibit activation of the phosphatidylinositol 3-kinase/protein kinase B (AKT)/mammalian target of rapamycin (MTOR) pathway through Tyr-1148 and Tyr-1068 and suppression of IRS-1 Ser-612, altered heterodimerization with ERBB2, reduced response to transforming growth factor beta suppression, and reduced ubiquitination/degradation of the EGFR through EGFR Tyr-1045, thus providing a survival advantage. Serine 262-265 protein tyrosine kinase 2 beta Homo sapiens 139-155 18557931-6 2008 As we have recently shown that protein kinase B/Akt is involved in the TNFalpha signalling pathway and promotes cell survival and skin carcinogenesis, we measured activatory phosphorylations of Akt (Ser-473 and Thr-308) and the signalling via related pathways Erk 1/2, p38 and p70-S6K. Serine 199-202 protein tyrosine kinase 2 beta Homo sapiens 31-47 18160431-6 2008 Hepatocytes infected with cell culture-grown HCV genotype 1a or 2a displayed a significant increase in the Ser(473) phosphorylation status of the Ser/Thr kinase protein kinase B (Akt/PKB), while Thr(308) phosphorylation was not significantly altered. Serine 107-110 protein tyrosine kinase 2 beta Homo sapiens 161-177 16836907-4 2006 RESULTS: The expression and transcription level of PKB (Ser(473)) in SACC-83 was lower than in SACC-LM (P < 0.05). Serine 56-59 protein tyrosine kinase 2 beta Homo sapiens 51-54 17336927-1 2007 Previously we identified an unusual potential dual Akt/protein kinase B consensus phosphorylation motif in the protein Synip (RxKxRS(97)xS(99)) with serine 99 as a unique Akt2, but not Akt1 or for Akt3, substrate phosphorylation site. Serine 149-155 protein tyrosine kinase 2 beta Homo sapiens 55-71 17030608-5 2006 Mutation of both serines to alanine uncouples BRF1 from PKB regulation, leading to constitutive mRNA decay even in the presence of stabilizing signals. Serine 17-24 protein tyrosine kinase 2 beta Homo sapiens 56-59 16234845-1 2006 Activation of protein kinase B (PKB, also known as Akt) by phosphorylation at serine-473 and threonine-308 promotes cell survival in multiple in vitro and in vivo models where neuronal death is seen, including traumatic brain injury (TBI); however, whether PKB is activated in humans after TBI was heretofore unknown. Serine 78-84 protein tyrosine kinase 2 beta Homo sapiens 14-30 16234845-1 2006 Activation of protein kinase B (PKB, also known as Akt) by phosphorylation at serine-473 and threonine-308 promotes cell survival in multiple in vitro and in vivo models where neuronal death is seen, including traumatic brain injury (TBI); however, whether PKB is activated in humans after TBI was heretofore unknown. Serine 78-84 protein tyrosine kinase 2 beta Homo sapiens 32-35 16836907-5 2006 The expression of PKB (Ser(473)) in SACC cell lines with LY294002 was lower than in SACC cell lines without LY294002 (P < 0.01). Serine 23-26 protein tyrosine kinase 2 beta Homo sapiens 18-21 16836907-7 2006 CONCLUSIONS: In vitro, the protein expression and transcription level of PKB (Ser(473)) is higher in SACC-LM cell line than in SACC-83 cell line. Serine 78-81 protein tyrosine kinase 2 beta Homo sapiens 73-76 16836907-8 2006 In vitro, LY294002 can inhibit protein expression of PKB (Ser(473)) in SACC-83 cells and SACC-LM cells. Serine 58-61 protein tyrosine kinase 2 beta Homo sapiens 53-56 16543730-5 2006 Western blotting using an antibody specific for the PKB/SGK1 consensus phosphorylation site in GSK3a/beta (serine 21 and 9 respectively) revealed an increase in GSK3a/beta phosphorylation in human embryonic kidney 293 (HEK293) cells overexpressing wild type SGK1, constitutively active SGK1, but not catalytically inactive SGK1. Serine 107-113 protein tyrosine kinase 2 beta Homo sapiens 52-55 14637150-0 2003 Transforming growth factor-beta1 stimulated protein kinase B serine-473 and focal adhesion kinase tyrosine phosphorylation dependent on cell adhesion in human hepatocellular carcinoma SMMC-7721 cells. Serine 61-67 protein tyrosine kinase 2 beta Homo sapiens 44-60 16221682-1 2005 The insulin-signaling pathway leading to the activation of Akt/protein kinase B has been well characterized except for a single step, the phosphorylation of Akt at Ser-473. Serine 164-167 protein tyrosine kinase 2 beta Homo sapiens 63-79 15026413-4 2004 The main objective of the present study was to investigate the potential involvement of the serine threonine kinase Akt (also known as protein kinase B) signaling pathway in the mechanisms of beta-synuclein neuroprotection. Serine 92-98 protein tyrosine kinase 2 beta Homo sapiens 135-151 14963038-7 2004 Pyk2 expression in HeLa cells induces Stat3 reporter gene activation and Stat3 phosphorylation on amino acid residues Tyr-705 and Ser-727. Serine 130-133 protein tyrosine kinase 2 beta Homo sapiens 0-4 14637150-10 2003 The above results indicate that PKB-Ser-473 and FAK-Tyr phosphorylation stimulated by TGF-beta1 are both dependent on cell adhesion. Serine 36-39 protein tyrosine kinase 2 beta Homo sapiens 32-35 16129678-4 2005 Studying the function of this phosphorylation, we found that replacing Ser(318) by alanine completely prevented both the attenuation of insulin-stimulated Akt/protein kinase B Ser(473) phosphorylation and glucose uptake after 60 min of insulin stimulation. Serine 71-74 protein tyrosine kinase 2 beta Homo sapiens 159-175 16129678-5 2005 Unexpectedly, after acute insulin stimulation, we observed that phosphorylation of Ser(318) is not inhibitory but rather enhances insulin signal transduction because introduction of Ala(318) led to a reduction of the insulin-stimulated Akt/protein kinase B phosphorylation. Serine 83-86 protein tyrosine kinase 2 beta Homo sapiens 240-256 15538381-3 2004 Here we report that protein kinase B (PKB/Akt) stabilizes ARE transcripts by phosphorylating BRF1 at serine 92 (S92). Serine 101-107 protein tyrosine kinase 2 beta Homo sapiens 20-36 15169778-3 2004 Here we show that PKB inhibits Mdm2 self-ubiquitination via phosphorylation of Mdm2 on Ser(166) and Ser(188). Serine 87-90 protein tyrosine kinase 2 beta Homo sapiens 18-21 15169778-3 2004 Here we show that PKB inhibits Mdm2 self-ubiquitination via phosphorylation of Mdm2 on Ser(166) and Ser(188). Serine 100-103 protein tyrosine kinase 2 beta Homo sapiens 18-21 15169778-5 2004 Introduction of a constitutively active form of PKB together with Mdm2 into cells induced phosphorylation of Mdm2 at Ser(166) and Ser(188) and stabilized Mdm2 protein. Serine 117-120 protein tyrosine kinase 2 beta Homo sapiens 48-51 15169778-5 2004 Introduction of a constitutively active form of PKB together with Mdm2 into cells induced phosphorylation of Mdm2 at Ser(166) and Ser(188) and stabilized Mdm2 protein. Serine 130-133 protein tyrosine kinase 2 beta Homo sapiens 48-51 14668339-5 2004 This activation is found in conjunction with an increased time-dependent phosphorylation of the downstream kinase AKT/protein kinase B on both Ser(473) and Thr(308) residues. Serine 143-146 protein tyrosine kinase 2 beta Homo sapiens 118-134 14637150-4 2003 We found that PKB-Ser-473 phosphorylation was apparently up-regulated by TGF-beta1. Serine 18-21 protein tyrosine kinase 2 beta Homo sapiens 14-17 14637150-7 2003 We observed that integrin-linked kinase (ILK) was also up-regulated by TGF-beta1 in good accordance with PKB-Ser-473 phosphorylation. Serine 109-112 protein tyrosine kinase 2 beta Homo sapiens 105-108 14637150-8 2003 We first found that TGF-beta1 could stimulate PKB-Ser-473 phosphorylation possibly via up-regulating ILK expression. Serine 50-53 protein tyrosine kinase 2 beta Homo sapiens 46-49 12435806-9 2002 Likewise, HU-210 stimulated the PI3K downstream targets protein kinase B (PKB), as shown by its phosphorylation in Thr 308 and Ser 473 residues, and Raf-1. Serine 127-130 protein tyrosine kinase 2 beta Homo sapiens 56-72 12624792-1 2003 Protein kinase B (PKB) is an important intermediate in the phosphatidylinositol-3 kinase signaling cascade that acts to phosphorylate glycogen synthase kinase-3 (GSK-3) at its serine 9 residue, thereby inactivating it. Serine 176-182 protein tyrosine kinase 2 beta Homo sapiens 0-16 12624792-1 2003 Protein kinase B (PKB) is an important intermediate in the phosphatidylinositol-3 kinase signaling cascade that acts to phosphorylate glycogen synthase kinase-3 (GSK-3) at its serine 9 residue, thereby inactivating it. Serine 176-182 protein tyrosine kinase 2 beta Homo sapiens 18-21 12435806-9 2002 Likewise, HU-210 stimulated the PI3K downstream targets protein kinase B (PKB), as shown by its phosphorylation in Thr 308 and Ser 473 residues, and Raf-1. Serine 127-130 protein tyrosine kinase 2 beta Homo sapiens 74-77 12196460-9 2002 IRK and PI3K activities were not altered by troglitazone, but PKB Ser(473) phosphorylation was enhanced compared with pretreatment and placebo at the clamp insulin level (138 +/- 36 vs. 77 +/- 16 and 55 +/- 13 internal standard units; both P < 0.05) and with pretreatment at the basal level (31 +/- 9 vs. 14 +/- 4 internal standard units; P < 0.05). Serine 66-69 protein tyrosine kinase 2 beta Homo sapiens 62-65 12107176-8 2002 Taken together, these results demonstrate that serine 454 of ATP-citrate lyase is a novel and major in vivo substrate for protein kinase B. Serine 47-53 protein tyrosine kinase 2 beta Homo sapiens 122-138 11707620-7 2001 The substrate specificity of SGK isoforms superficially resembles that of PKB in that serine and threonine residues lying in Arg-Xaa-Arg-Xaa-Xaa-Ser/Thr sequences (where Xaa is a variable amino acid) are phosphorylated. Serine 86-92 protein tyrosine kinase 2 beta Homo sapiens 74-77 12000750-4 2002 This effect was correlated with inhibition of the phosphorylation of the PDK1 downstream substrate Akt/protein kinase B (PKB) on two regulatory sites, Thr(308) and Ser(473). Serine 164-167 protein tyrosine kinase 2 beta Homo sapiens 103-119 11809746-5 2002 Insulin-stimulated serine phosphorylation of Akt/protein kinase B was significantly impaired in mutant-transfected cells. Serine 19-25 protein tyrosine kinase 2 beta Homo sapiens 49-65 12167717-3 2002 PKB induction requires phosphorylation of two critical residues, threonine 308 in the activation loop and serine 473 near the carboxyl terminus. Serine 106-112 protein tyrosine kinase 2 beta Homo sapiens 0-3 12167717-4 2002 Membrane localization of PKB was found to be a primary determinant of serine 473 phosphorylation. Serine 70-76 protein tyrosine kinase 2 beta Homo sapiens 25-28 12164932-6 2002 Both serine-473 and threonine-308 sites of protein kinase B/Akt show increased phosphorylation in ganglioside-deficient cells, but the cell survival correlates with increased phosphorylation of the serine-473 site of Akt, not with increased phosphorylation of the threonine-308 site. Serine 5-11 protein tyrosine kinase 2 beta Homo sapiens 43-59 12164932-6 2002 Both serine-473 and threonine-308 sites of protein kinase B/Akt show increased phosphorylation in ganglioside-deficient cells, but the cell survival correlates with increased phosphorylation of the serine-473 site of Akt, not with increased phosphorylation of the threonine-308 site. Serine 198-204 protein tyrosine kinase 2 beta Homo sapiens 43-59 12164932-7 2002 Consistently, blockade of ganglioside GT1b function activates integrin-linked kinase and only the serine-473 site of protein kinase B/Akt. Serine 98-104 protein tyrosine kinase 2 beta Homo sapiens 117-133 11897496-1 2002 The biochemical mechanism of apoptosis induced by ceramide remains still unclear, although it has been reported that dephosphorylation of PKB at Ser-473 may be a key event. Serine 145-148 protein tyrosine kinase 2 beta Homo sapiens 138-141 11884412-1 2002 We have investigated the effects of insulin, amino acids, and the degree of muscle loading on the phosphorylation of Ser(2448), a site in the mammalian target of rapamycin (mTOR) phosphorylated by protein kinase B (PKB) in vitro. Serine 117-120 protein tyrosine kinase 2 beta Homo sapiens 197-213 11884412-1 2002 We have investigated the effects of insulin, amino acids, and the degree of muscle loading on the phosphorylation of Ser(2448), a site in the mammalian target of rapamycin (mTOR) phosphorylated by protein kinase B (PKB) in vitro. Serine 117-120 protein tyrosine kinase 2 beta Homo sapiens 215-218 11707620-7 2001 The substrate specificity of SGK isoforms superficially resembles that of PKB in that serine and threonine residues lying in Arg-Xaa-Arg-Xaa-Xaa-Ser/Thr sequences (where Xaa is a variable amino acid) are phosphorylated. Serine 145-148 protein tyrosine kinase 2 beta Homo sapiens 74-77 10722653-1 2000 The function of Akt (protein kinase B) is regulated by phosphorylation on two sites conserved within the AGC kinase family: the activation loop (Thr-308) in the kinase core and a hydrophobic phosphorylation site on the carboxyl terminus (Ser-473). Serine 238-241 protein tyrosine kinase 2 beta Homo sapiens 21-37 11679435-6 2001 C2-ceramide completely abolished insulin-stimulated Akt/protein kinase B phosphorylation on regulatory residues Thr 308 and Ser 473, as did TNF-alpha, and inhibited insulin-induced mobility shift in Akt1 and Akt2 separated in PAGE. Serine 124-127 protein tyrosine kinase 2 beta Homo sapiens 56-72 11598301-2 2001 PKB is activated by phosphorylation on residues threonine 308, by the protein kinase PDK1, and Serine 473, by a putative serine 473 kinase. Serine 95-101 protein tyrosine kinase 2 beta Homo sapiens 0-3 11535620-5 2001 Nuclear exclusion of AHNAK is mediated through a nuclear export signal (NES) in a manner that depends on the phosphorylation of serine 5535 of AHNAK by PKB, a process that also plays a major role in determining extranuclear localization of AHNAK. Serine 128-134 protein tyrosine kinase 2 beta Homo sapiens 152-155 11313365-0 2001 Regulation of protein kinase B/Akt-serine 473 phosphorylation by integrin-linked kinase: critical roles for kinase activity and amino acids arginine 211 and serine 343. Serine 35-41 protein tyrosine kinase 2 beta Homo sapiens 14-30 11313365-0 2001 Regulation of protein kinase B/Akt-serine 473 phosphorylation by integrin-linked kinase: critical roles for kinase activity and amino acids arginine 211 and serine 343. Serine 157-163 protein tyrosine kinase 2 beta Homo sapiens 14-30 11319202-2 2001 Stimulating ECs with vascular endothelial growth factor (VEGF) leads to the activation of Akt/protein kinase B, which in turn activates endothelial nitric oxide synthase (eNOS) by phosphorylation on serine 1177. Serine 199-205 protein tyrosine kinase 2 beta Homo sapiens 94-110 11035810-2 2000 These serine residues of GSK-3 have been previously identified as targets of protein kinase B (PKB/Akt), a serine/threonine kinase located downstream of phosphatidylinositol 3-kinase. Serine 6-12 protein tyrosine kinase 2 beta Homo sapiens 77-93 10874027-3 2000 Here we report that the major PKB subtype in platelets is PKBalpha, which is activated by phosphorylation of Thr(308) and Ser(473) and has a constitutively phosphorylated Thr(450) that does not contribute to PKB activation. Serine 122-125 protein tyrosine kinase 2 beta Homo sapiens 30-33 10874027-3 2000 Here we report that the major PKB subtype in platelets is PKBalpha, which is activated by phosphorylation of Thr(308) and Ser(473) and has a constitutively phosphorylated Thr(450) that does not contribute to PKB activation. Serine 122-125 protein tyrosine kinase 2 beta Homo sapiens 58-61 10874027-8 2000 These data reveal a PI-3K-independent PKB activation in which PKCalpha/beta regulates the phosphorylation of Ser(473) in PKBalpha. Serine 109-112 protein tyrosine kinase 2 beta Homo sapiens 38-41 10788440-0 2000 Ceramide inhibits protein kinase B/Akt by promoting dephosphorylation of serine 473. Serine 73-79 protein tyrosine kinase 2 beta Homo sapiens 18-34 10788440-7 2000 Two residues of PKB (threonine 308 and serine 473) require phosphorylation for maximal PKB activation. Serine 39-45 protein tyrosine kinase 2 beta Homo sapiens 16-19 10788440-7 2000 Two residues of PKB (threonine 308 and serine 473) require phosphorylation for maximal PKB activation. Serine 39-45 protein tyrosine kinase 2 beta Homo sapiens 87-90 11733864-2 2001 In most cells, three serine/threonine moieties in FKHR family members are phosphorylated after insulin treatment or protein kinase B/Akt (PKB) transfection, and each of the three phosphorylated PKB sites contributes to insulin- or PKB-mediated inhibition of both the action and the nuclear localization of FKHR family members. Serine 21-27 protein tyrosine kinase 2 beta Homo sapiens 116-132 11733864-2 2001 In most cells, three serine/threonine moieties in FKHR family members are phosphorylated after insulin treatment or protein kinase B/Akt (PKB) transfection, and each of the three phosphorylated PKB sites contributes to insulin- or PKB-mediated inhibition of both the action and the nuclear localization of FKHR family members. Serine 21-27 protein tyrosine kinase 2 beta Homo sapiens 138-141 11733864-2 2001 In most cells, three serine/threonine moieties in FKHR family members are phosphorylated after insulin treatment or protein kinase B/Akt (PKB) transfection, and each of the three phosphorylated PKB sites contributes to insulin- or PKB-mediated inhibition of both the action and the nuclear localization of FKHR family members. Serine 21-27 protein tyrosine kinase 2 beta Homo sapiens 194-197 11733864-2 2001 In most cells, three serine/threonine moieties in FKHR family members are phosphorylated after insulin treatment or protein kinase B/Akt (PKB) transfection, and each of the three phosphorylated PKB sites contributes to insulin- or PKB-mediated inhibition of both the action and the nuclear localization of FKHR family members. Serine 21-27 protein tyrosine kinase 2 beta Homo sapiens 194-197 11373274-0 2001 Insulin-stimulated protein kinase B phosphorylation on Ser-473 is independent of its activity and occurs through a staurosporine-insensitive kinase. Serine 55-58 protein tyrosine kinase 2 beta Homo sapiens 19-35 11373274-1 2001 Full activation of protein kinase B (PKB, also called Akt) requires phosphorylation on two regulatory sites, Thr-308 in the activation loop and Ser-473 in the hydrophobic C-terminal regulatory domain (numbering for PKB alpha/Akt-1). Serine 144-147 protein tyrosine kinase 2 beta Homo sapiens 19-35 11373274-1 2001 Full activation of protein kinase B (PKB, also called Akt) requires phosphorylation on two regulatory sites, Thr-308 in the activation loop and Ser-473 in the hydrophobic C-terminal regulatory domain (numbering for PKB alpha/Akt-1). Serine 144-147 protein tyrosine kinase 2 beta Homo sapiens 37-40 11373274-4 2001 We found that staurosporine, a broad-specificity kinase inhibitor and inducer of cell apoptosis, attenuated PKB activation exclusively through the inhibition of Thr-308 phosphorylation, with Ser-473 phosphorylation unaffected. Serine 191-194 protein tyrosine kinase 2 beta Homo sapiens 108-111 11373274-7 2001 These data indicate that agonist-induced phosphorylation of Ser-473 of PKB is independent of PDK1 or PKB activity and occurs through a distinct Ser-473 kinase that is not inhibited by staurosporine. Serine 60-63 protein tyrosine kinase 2 beta Homo sapiens 71-74 11373274-7 2001 These data indicate that agonist-induced phosphorylation of Ser-473 of PKB is independent of PDK1 or PKB activity and occurs through a distinct Ser-473 kinase that is not inhibited by staurosporine. Serine 144-147 protein tyrosine kinase 2 beta Homo sapiens 71-74 11116148-10 2001 Based on a consensus sequence, we identified a PKB phosphorylation site (Ser(1834)) within the region of p300/CBP known to bind C/EBPbeta. Serine 73-76 protein tyrosine kinase 2 beta Homo sapiens 47-50 11043856-10 2000 Furthermore, insulin-stimulated translocation of protein kinase B/Akt to the plasma membrane, where serine phosphorylation and full activation occurs, is impaired in Type II diabetes. Serine 100-106 protein tyrosine kinase 2 beta Homo sapiens 49-65 10898514-6 2000 The down-regulation of cyclin D3 was at the level of translation, and this event was preceded by a pronounced inhibition of Akt/protein kinase B phosphorylation at Ser 473. Serine 164-167 protein tyrosine kinase 2 beta Homo sapiens 128-144 10567225-5 1999 In the present work, we have found that PKB directly phosphorylates mTOR and, using phosphospecific antibodies, we have shown this phosphorylation occurs at Ser(2448). Serine 157-160 protein tyrosine kinase 2 beta Homo sapiens 40-43 10637513-0 1999 Integrin-linked kinase regulates phosphorylation of serine 473 of protein kinase B by an indirect mechanism. Serine 52-58 protein tyrosine kinase 2 beta Homo sapiens 66-82 10637513-2 1999 Full activation of protein kinase B requires 3-phosphoinositides and dual phosphorylation on threonine-308 and serine-473. Serine 111-117 protein tyrosine kinase 2 beta Homo sapiens 19-35 10637513-5 1999 Consistent with this, in a model COS cell system we show that expression of wild-type integrin-linked kinase promotes the wortmannin sensitive phosphorylation of serine-473 of protein kinase B and its downstream substrates, and inhibits C2-ceramide induced apoptosis. Serine 162-168 protein tyrosine kinase 2 beta Homo sapiens 176-192 10637513-8 1999 Mutation of this serine to aspartate or glutamate, but not alanine, in combination with the inactivating lysine mutation restores integrin-linked kinase dependent phosphorylation of serine-473 of protein kinase B. Serine 17-23 protein tyrosine kinase 2 beta Homo sapiens 196-212 10637513-8 1999 Mutation of this serine to aspartate or glutamate, but not alanine, in combination with the inactivating lysine mutation restores integrin-linked kinase dependent phosphorylation of serine-473 of protein kinase B. Serine 182-188 protein tyrosine kinase 2 beta Homo sapiens 196-212 10601311-2 1999 Both of these kinases are activated in cells by phosphorylation of a conserved residue in the kinase domain (Thr-308 of protein kinase B (PKB) and Thr-252 of p70 S6 kinase) and another conserved residue located C-terminal to the kinase domain (Ser-473 of PKB and Thr-412 of p70 S6 kinase). Serine 244-247 protein tyrosine kinase 2 beta Homo sapiens 120-136 10601311-2 1999 Both of these kinases are activated in cells by phosphorylation of a conserved residue in the kinase domain (Thr-308 of protein kinase B (PKB) and Thr-252 of p70 S6 kinase) and another conserved residue located C-terminal to the kinase domain (Ser-473 of PKB and Thr-412 of p70 S6 kinase). Serine 244-247 protein tyrosine kinase 2 beta Homo sapiens 138-141 10567225-6 1999 Insulin also induces phosphorylation on Ser(2448) and this effect is blocked by wortmannin but not rapamycin, consistent with the effect being mediated by PKB. Serine 40-43 protein tyrosine kinase 2 beta Homo sapiens 155-158 10567225-8 1999 Our findings demonstrate that mTOR is a direct target for PKB and support the conclusion that regulation of phosphorylation of Ser(2448) is a point of convergence for the counteracting regulatory effects of growth factors and amino acid levels. Serine 127-130 protein tyrosine kinase 2 beta Homo sapiens 58-61 10576742-2 1999 Akt (protein kinase B), a member of a different signaling pathway that also regulates these responses, interacted with Raf and phosphorylated this protein at a highly conserved serine residue in its regulatory domain in vivo. Serine 177-183 protein tyrosine kinase 2 beta Homo sapiens 5-21 10601992-1 1999 The proline-, glutamic acid-, serine- and threonine-enriched protein tyrosine phosphatase PEP, which is expressed primarily in hematopoietic cells, was recently discovered to be physically associated with the 50-kDa cytosolic protein tyrosine kinase (PTK) Csk, an important suppressor of Src family PTK, including Lck and Fyn in T cells. Serine 30-36 protein tyrosine kinase 2 beta Homo sapiens 251-254 10601992-1 1999 The proline-, glutamic acid-, serine- and threonine-enriched protein tyrosine phosphatase PEP, which is expressed primarily in hematopoietic cells, was recently discovered to be physically associated with the 50-kDa cytosolic protein tyrosine kinase (PTK) Csk, an important suppressor of Src family PTK, including Lck and Fyn in T cells. Serine 30-36 protein tyrosine kinase 2 beta Homo sapiens 299-302 10377430-3 1999 Protein kinase B (PKB)/Akt, a key mediator of PI3 kinase signal transduction, phosphorylated recombinant FKHR1 in vitro at threonine-24 and serine-253. Serine 140-146 protein tyrosine kinase 2 beta Homo sapiens 0-16 10497255-4 1999 Furthermore, PKB and IRS-1 formed stable complexes in vivo, and overexpression of PKB enhanced Ser phosphorylation of IRS-1. Serine 95-98 protein tyrosine kinase 2 beta Homo sapiens 82-85 10497255-8 1999 In contrast, insulin-stimulated PKB-mediated phosphorylation of Ser residues within the phosphotyrosine binding/SAIN domain of IRS-1 protects IRS-1 from the rapid action of protein-tyrosine phosphatases and enables it to maintain its Tyr-phosphorylated active conformation. Serine 64-67 protein tyrosine kinase 2 beta Homo sapiens 32-35 10377430-3 1999 Protein kinase B (PKB)/Akt, a key mediator of PI3 kinase signal transduction, phosphorylated recombinant FKHR1 in vitro at threonine-24 and serine-253. Serine 140-146 protein tyrosine kinase 2 beta Homo sapiens 18-21 7600304-4 1995 cDNA expression studies further revealed that this association is critical for the IL-2-induced activation of Syk PTK, which occurs primarily via the serine-rich region of the IL-2R beta chain, which is essential for proliferative signal transmission. Serine 150-156 protein tyrosine kinase 2 beta Homo sapiens 114-117 10208883-0 1999 Identification of a human Akt3 (protein kinase B gamma) which contains the regulatory serine phosphorylation site. Serine 86-92 protein tyrosine kinase 2 beta Homo sapiens 32-48 10187855-6 1999 The effect of this finding on the downstream insulin signal was demonstrated by a 90% reduction in insulin stimulated protein kinase B (PKB) serine 473 phosphorylation and impaired activation of PKBalpha and PKBgamma. Serine 141-147 protein tyrosine kinase 2 beta Homo sapiens 118-134 10187855-6 1999 The effect of this finding on the downstream insulin signal was demonstrated by a 90% reduction in insulin stimulated protein kinase B (PKB) serine 473 phosphorylation and impaired activation of PKBalpha and PKBgamma. Serine 141-147 protein tyrosine kinase 2 beta Homo sapiens 136-139 9202156-6 1997 Evidence for this is that a constitutive active PKB, T308D, S473D, containing activating mutations in the serine and threonine residues which are phosphorylated subsequent to PI3-kinase activation, cannot be further stimulated by cAMP elevations. Serine 106-112 protein tyrosine kinase 2 beta Homo sapiens 48-51 7637810-4 1995 Activation of PKB was inhibited by the phosphatidylinositol-3-OH kinase (PI(3)K) inhibitor wortmannin and by coexpression of a dominant-negative mutant of PI(3)K. PDGF receptor mutants that lack detectable associated PI(3)K activity also fail to induce PKB activation, PKB kinase activity is correlated with phosphorylation of PKB on serine. Serine 334-340 protein tyrosine kinase 2 beta Homo sapiens 14-17 7518463-4 1994 Individual replacement of the two cysteine residues within the first 10 amino acids of p59fyn and p56lck with serine indicated that Cys3 was the major determinant of palmitoylation, as well as association of the PTK with glycosyl-phosphatidylinositol-anchored proteins. Serine 110-116 protein tyrosine kinase 2 beta Homo sapiens 212-215 35607451-14 2022 Interestingly, melatonin induced the phosphorylation of protein kinase B/AKT at serine 473 residue and GSK-3beta at serine 9 residue. Serine 80-86 protein tyrosine kinase 2 beta Homo sapiens 56-72 8260646-10 1993 Interestingly the "serine-rich" and "acidic" regions of IL-2R beta are both required for the PTK activation of p56lck. Serine 19-25 protein tyrosine kinase 2 beta Homo sapiens 93-96 33854635-6 2021 Mechanistically, SAA1 knockdown can inhibit serine/threonine protein kinase B (AKT) phosphorylation, thereby regulating the expression of apoptosis-related proteins such as Bcl2 and Bax, leading to GBM cell death. Serine 44-50 protein tyrosine kinase 2 beta Homo sapiens 61-77 35124166-4 2022 Here, we showed protein kinase B/Akt1, a serine/ threonine kinase implicated in schizophrenia, phosphorylated dysbindin-1A at serine 10 (S10). Serine 41-47 protein tyrosine kinase 2 beta Homo sapiens 16-32 35330934-1 2022 The serine/threonine kinase Akt, also known as protein kinase B (PKB), is one of the key factors regulating glucose and lipid energy metabolism, and is the core focus of current research on diabetes and metabolic diseases. Serine 4-10 protein tyrosine kinase 2 beta Homo sapiens 47-63 35330934-1 2022 The serine/threonine kinase Akt, also known as protein kinase B (PKB), is one of the key factors regulating glucose and lipid energy metabolism, and is the core focus of current research on diabetes and metabolic diseases. Serine 4-10 protein tyrosine kinase 2 beta Homo sapiens 65-68 32120136-1 2020 The serine/threonine kinase AKT, also known as protein kinase B (PKB), is the major substrate to phosphoinositide 3-kinase (PI3K) and consists of three paralogs: AKT1 (PKBalpha), AKT2 (PKBbeta) and AKT3 (PKBgamma). Serine 4-10 protein tyrosine kinase 2 beta Homo sapiens 47-63 33633735-3 2020 PKB has two phosphorylation sites, threonine 308 (T308) and serine 473 (S473), which can be phosphorylated by phosphoinositide-dependent protein kinase-1 (PDK1) and mTORC2, respectively. Serine 60-66 protein tyrosine kinase 2 beta Homo sapiens 0-3 32760285-9 2020 In addition, PPARdelta activation recovered EtOH-induced loss of protein kinase B (AKT) phosphorylation at serine 473 via rapamycin-insensitive companion of mammalian target of rapamycin (Rictor) activation. Serine 107-113 protein tyrosine kinase 2 beta Homo sapiens 65-81 32120136-1 2020 The serine/threonine kinase AKT, also known as protein kinase B (PKB), is the major substrate to phosphoinositide 3-kinase (PI3K) and consists of three paralogs: AKT1 (PKBalpha), AKT2 (PKBbeta) and AKT3 (PKBgamma). Serine 4-10 protein tyrosine kinase 2 beta Homo sapiens 65-68 31134093-3 2019 These neutrophil alterations include marked defects in intracellular signaling pathways involving serine/threonine kinases such as protein kinase B (AKT), p38-mitogen-activated protein kinase (MAPK), and the MAP kinases1/2; activation of the NADPH oxidase complex; myeloperoxidase (MPO) release; and bactericidal activity of neutrophils stimulated by the bacterial peptide formyl-Methionine-Leucine-Phenylalanine (fMLF). Serine 98-104 protein tyrosine kinase 2 beta Homo sapiens 131-147