PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 33712428-5 2021 Overexpression of Haa1p or Tye7p improved xylose consumption rate by nearly 50%, while the ethanol yield enhanced by nearly 8% under acetic acid and furfural stress conditions. Acetic Acid 133-144 Haa1p Saccharomyces cerevisiae S288C 18-23 34477863-0 2021 Development of an Haa1-based biosensor for acetic acid sensing in Saccharomyces cerevisiae. Acetic Acid 43-54 Haa1p Saccharomyces cerevisiae S288C 18-22 34477863-5 2021 Here, we present the development of an acetic acid biosensor based on the Saccharomyces cerevisiae transcription factor Haa1 that upon binding to acetic acid relocates to the nucleus. Acetic Acid 39-50 Haa1p Saccharomyces cerevisiae S288C 120-124 34477863-5 2021 Here, we present the development of an acetic acid biosensor based on the Saccharomyces cerevisiae transcription factor Haa1 that upon binding to acetic acid relocates to the nucleus. Acetic Acid 146-157 Haa1p Saccharomyces cerevisiae S288C 120-124 33712428-6 2021 Co-overexpression of Haa1p and Tye7p resulted in 59% increase in xylose consumption rate and 12% increase in ethanol yield, revealing the beneficial effects of Haa1p and Tye7p on improving the tolerance of yeast to mixed acetic acid and furfural.IMPORTANCEInhibitor tolerance is essential for S. cerevisiae when fermenting lignocellulosic hydrolysate with various inhibitors, including weak acids, furans, and phenols. Acetic Acid 221-232 Haa1p Saccharomyces cerevisiae S288C 21-26 33712428-6 2021 Co-overexpression of Haa1p and Tye7p resulted in 59% increase in xylose consumption rate and 12% increase in ethanol yield, revealing the beneficial effects of Haa1p and Tye7p on improving the tolerance of yeast to mixed acetic acid and furfural.IMPORTANCEInhibitor tolerance is essential for S. cerevisiae when fermenting lignocellulosic hydrolysate with various inhibitors, including weak acids, furans, and phenols. Acetic Acid 221-232 Haa1p Saccharomyces cerevisiae S288C 160-165 33712428-9 2021 The transcription factor Haa1p was found to be involved in both acetic acid and furfural tolerance. Acetic Acid 64-75 Haa1p Saccharomyces cerevisiae S288C 25-30 30476185-5 2019 We show that DNA binding by Haa1 is induced in the presence of acetic acid and that the N-terminal Zn-binding domain is essential for this activity. Acetic Acid 63-74 Haa1p Saccharomyces cerevisiae S288C 28-32 29607452-0 2018 HAA1 and PRS3 overexpression boosts yeast tolerance towards acetic acid improving xylose or glucose consumption: unravelling the underlying mechanisms. Acetic Acid 60-71 Haa1p Saccharomyces cerevisiae S288C 0-4 30237501-2 2018 ZbHaa1 is the functional homologue of S. cerevisiae Haa1 and a bifunctional transcription factor able to modulate Z. bailii adaptive response to acetic acid and copper stress. Acetic Acid 145-156 Haa1p Saccharomyces cerevisiae S288C 2-6 29607452-2 2018 In this work, overexpression of a weak acid stress transcriptional activator encoded by the gene HAA1 and a phosphoribosyl pyrophosphate synthetase encoded by PRS3 in a recombinant industrial Saccharomyces cerevisiae strain containing a xylose metabolic pathway was evaluated in the presence of acetic acid in xylose- or glucose-containing media. Acetic Acid 295-306 Haa1p Saccharomyces cerevisiae S288C 97-101 29607452-3 2018 HAA1 or PRS3 overexpression resulted in superior yeast growth and higher sugar consumption capacities in the presence of 4 g/L acetic acid, and a positive synergistic effect resulted from the simultaneous overexpression of both genes. Acetic Acid 127-138 Haa1p Saccharomyces cerevisiae S288C 0-4 29607452-5 2018 Furthermore, the overexpression of HAA1 and/or PRS3 was found to increase the robustness of yeast cell wall when challenged with acetic acid stress, suggesting the involvement of the modulation of the cell wall integrity pathway. Acetic Acid 129-140 Haa1p Saccharomyces cerevisiae S288C 35-39 29607452-6 2018 This study clearly shows HAA1 and/or, for the first time, PRS3 overexpression to play an important role in the improvement of industrial yeast tolerance towards acetic acid. Acetic Acid 161-172 Haa1p Saccharomyces cerevisiae S288C 25-29 28068993-2 2017 The engineering of Haa1 either by overexpression or mutagenesis has therefore been considered to be a promising avenue towards the construction of more robust strains with improved acetic acid tolerance. Acetic Acid 181-192 Haa1p Saccharomyces cerevisiae S288C 19-23 28432100-12 2017 By understanding how Haa1 is regulated, we can make advances in the field of food sciences to better preserve food and engineer acetic acid-resistant strains that will increase productivity in the biofuel industry. Acetic Acid 128-139 Haa1p Saccharomyces cerevisiae S288C 21-25 28428821-6 2017 Among these genes is HAA1 encoding the main transcriptional regulator of yeast transcriptome reprograming in response to acetic acid and genes of the Haa1-regulon; all demonstrated determinants of acetic acid tolerance. Acetic Acid 121-132 Haa1p Saccharomyces cerevisiae S288C 21-25 28428821-6 2017 Among these genes is HAA1 encoding the main transcriptional regulator of yeast transcriptome reprograming in response to acetic acid and genes of the Haa1-regulon; all demonstrated determinants of acetic acid tolerance. Acetic Acid 197-208 Haa1p Saccharomyces cerevisiae S288C 21-25 28428821-6 2017 Among these genes is HAA1 encoding the main transcriptional regulator of yeast transcriptome reprograming in response to acetic acid and genes of the Haa1-regulon; all demonstrated determinants of acetic acid tolerance. Acetic Acid 197-208 Haa1p Saccharomyces cerevisiae S288C 150-154 28068993-0 2017 Improvement of yeast tolerance to acetic acid through Haa1 transcription factor engineering: towards the underlying mechanisms. Acetic Acid 34-45 Haa1p Saccharomyces cerevisiae S288C 54-58 28068993-1 2017 BACKGROUND: Besides being a major regulator of the response to acetic acid in Saccharomyces cerevisiae, the transcription factor Haa1 is an important determinant of the tolerance to this acid. Acetic Acid 63-74 Haa1p Saccharomyces cerevisiae S288C 129-133 28086780-0 2017 The Zygosaccharomyces bailii transcription factor Haa1 is required for acetic acid and copper stress responses suggesting subfunctionalization of the ancestral bifunctional protein Haa1/Cup2. Acetic Acid 71-82 Haa1p Saccharomyces cerevisiae S288C 50-54 28086780-2 2017 In Saccharomyces cerevisiae, the transcription factor Haa1 (ScHaa1) is considered the main player in genomic expression reprogramming in response to acetic acid stress, but the role of its homologue in Z. bailii (ZbHaa1) is unknown. Acetic Acid 149-160 Haa1p Saccharomyces cerevisiae S288C 54-58 28068993-3 2017 RESULTS: By applying the concept of global transcription machinery engineering to the regulon-specific transcription factor Haa1, a mutant allele containing two point mutations could be selected that resulted in a significantly higher acetic acid tolerance as compared to the wild-type allele. Acetic Acid 235-246 Haa1p Saccharomyces cerevisiae S288C 124-128 28068993-7 2017 While the rapid Haa1 translocation from the cytosol to the nucleus in response to acetic acid was not affected in the Haa1S135F mutant strain, the levels of transcriptional activation of four selected Haa1-target genes by acetic acid were significantly higher in cells of the mutant strain as compared to cells of the wild-type strain. Acetic Acid 82-93 Haa1p Saccharomyces cerevisiae S288C 16-20 28068993-9 2017 CONCLUSION: Our data confirms that engineering of the regulon-specific transcription factor Haa1 allows the improvement of acetic acid tolerance in S. cerevisiae. Acetic Acid 123-134 Haa1p Saccharomyces cerevisiae S288C 92-96 26673744-5 2015 This screen was based on the transformation of an acetic acid susceptible Saccharomyces cerevisiae mutant deleted for the gene encoding the acetic acid resistance determinant transcription factor Haa1. Acetic Acid 50-61 Haa1p Saccharomyces cerevisiae S288C 196-200 26740819-7 2016 This resulted in disappearance in the QTL mapping with the F7 segregants of a major F1 QTL, in which we identified HAA1, a known regulator of high acetic acid tolerance, as a true causative allele. Acetic Acid 147-158 Haa1p Saccharomyces cerevisiae S288C 115-119 26740819-9 2016 The superior HAA1 allele contained a unique single point mutation that significantly improved acetic acid tolerance under industrially relevant conditions when inserted into an industrial yeast strain for second-generation bioethanol production. Acetic Acid 94-105 Haa1p Saccharomyces cerevisiae S288C 13-17 26673744-5 2015 This screen was based on the transformation of an acetic acid susceptible Saccharomyces cerevisiae mutant deleted for the gene encoding the acetic acid resistance determinant transcription factor Haa1. Acetic Acid 140-151 Haa1p Saccharomyces cerevisiae S288C 196-200 25282639-0 2015 Improved ethanol production from xylose in the presence of acetic acid by the overexpression of the HAA1 gene in Saccharomyces cerevisiae. Acetic Acid 59-70 Haa1p Saccharomyces cerevisiae S288C 100-104 25282639-5 2015 Here, an acetic acid-responsive transcriptional activator, HAA1, was overexpressed in a recombinant xylose-fermenting S. cerevisiae strain to yield BY4741X/HAA1. Acetic Acid 9-20 Haa1p Saccharomyces cerevisiae S288C 59-63 25282639-5 2015 Here, an acetic acid-responsive transcriptional activator, HAA1, was overexpressed in a recombinant xylose-fermenting S. cerevisiae strain to yield BY4741X/HAA1. Acetic Acid 9-20 Haa1p Saccharomyces cerevisiae S288C 156-160 22961896-0 2012 Enhancement of acetic acid tolerance in Saccharomyces cerevisiae by overexpression of the HAA1 gene, encoding a transcriptional activator. Acetic Acid 15-26 Haa1p Saccharomyces cerevisiae S288C 90-94 22961896-2 2012 Here we show that the constitutive HAA1-overexpressing strain acquired a higher level of acetic acid tolerance. Acetic Acid 89-100 Haa1p Saccharomyces cerevisiae S288C 35-39 22961896-3 2012 Under conditions of acetic acid stress, the intracellular level of acetic acid was significantly lower in HAA1-overexpressing cells than in the wild-type cells. Acetic Acid 20-31 Haa1p Saccharomyces cerevisiae S288C 106-110 22961896-3 2012 Under conditions of acetic acid stress, the intracellular level of acetic acid was significantly lower in HAA1-overexpressing cells than in the wild-type cells. Acetic Acid 67-78 Haa1p Saccharomyces cerevisiae S288C 106-110 21586585-0 2011 Identification of a DNA-binding site for the transcription factor Haa1, required for Saccharomyces cerevisiae response to acetic acid stress. Acetic Acid 122-133 Haa1p Saccharomyces cerevisiae S288C 66-70 21586585-1 2011 The transcription factor Haa1 is the main player in reprogramming yeast genomic expression in response to acetic acid stress. Acetic Acid 106-117 Haa1p Saccharomyces cerevisiae S288C 25-29 21586585-2 2011 Mapping of the promoter region of one of the Haa1-activated genes, TPO3, allowed the identification of an acetic acid responsive element (ACRE) to which Haa1 binds in vivo. Acetic Acid 106-117 Haa1p Saccharomyces cerevisiae S288C 45-49 21586585-2 2011 Mapping of the promoter region of one of the Haa1-activated genes, TPO3, allowed the identification of an acetic acid responsive element (ACRE) to which Haa1 binds in vivo. Acetic Acid 106-117 Haa1p Saccharomyces cerevisiae S288C 153-157 21586585-8 2011 The Haa1-dependent transcriptional regulatory network active in yeast response to acetic acid stress is proposed. Acetic Acid 82-93 Haa1p Saccharomyces cerevisiae S288C 4-8 22050823-5 2011 In baker"s yeast (Saccharomyces cerevisiae), resistance to high acetic acid is acquired partly by loss of the plasma membrane aquaglyceroporin that facilitates the passive diffusional entry of undissociated acid into cells (Fps1), and partly through a transcriptional response mediated by the transcription factor Haa1. Acetic Acid 64-75 Haa1p Saccharomyces cerevisiae S288C 314-318 20955010-0 2010 Genomic expression program involving the Haa1p-regulon in Saccharomyces cerevisiae response to acetic acid. Acetic Acid 95-106 Haa1p Saccharomyces cerevisiae S288C 41-46 20955010-1 2010 The alterations occurring in yeast genomic expression during early response to acetic acid and the involvement of the transcription factor Haa1p in this transcriptional reprogramming are described in this study. Acetic Acid 79-90 Haa1p Saccharomyces cerevisiae S288C 139-144 20955010-2 2010 Haa1p was found to regulate, directly or indirectly, the transcription of approximately 80% of the acetic acid-activated genes, suggesting that Haa1p is the main player in the control of yeast response to this weak acid. Acetic Acid 99-110 Haa1p Saccharomyces cerevisiae S288C 0-5 20955010-2 2010 Haa1p was found to regulate, directly or indirectly, the transcription of approximately 80% of the acetic acid-activated genes, suggesting that Haa1p is the main player in the control of yeast response to this weak acid. Acetic Acid 99-110 Haa1p Saccharomyces cerevisiae S288C 144-149 20955010-3 2010 The genes identified in this work as being activated in response to acetic acid in a Haa1p-dependent manner include protein kinases, multidrug resistance transporters, proteins involved in lipid metabolism, in nucleic acid processing, and proteins of unknown function. Acetic Acid 68-79 Haa1p Saccharomyces cerevisiae S288C 85-90 20955010-6 2010 The deletion of the HRK1 gene was found to lead to the increase of the accumulation of labeled acetic acid into acid-stressed yeast cells, suggesting that the role of both HAA1 and HRK1 in providing protection against acetic acid is, at least partially, related with their involvement in the reduction of intracellular acetate concentration. Acetic Acid 95-106 Haa1p Saccharomyces cerevisiae S288C 172-176 20955010-6 2010 The deletion of the HRK1 gene was found to lead to the increase of the accumulation of labeled acetic acid into acid-stressed yeast cells, suggesting that the role of both HAA1 and HRK1 in providing protection against acetic acid is, at least partially, related with their involvement in the reduction of intracellular acetate concentration. Acetic Acid 218-229 Haa1p Saccharomyces cerevisiae S288C 172-176 16176797-6 2005 The acetic acid-induced prolongation of the lag phase of unadapted cell populations lacking HAA1 or TPO3, compared with wild-type population, was correlated with the level of the acid accumulated into the stressed cells. Acetic Acid 4-15 Haa1p Saccharomyces cerevisiae S288C 92-96