PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 28325287-0 2017 Cholesterol-Containing Nuclease-Resistant siRNA Accumulates in Tumors in a Carrier-free Mode and Silences MDR1 Gene. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 106-110 30526541-7 2018 RESULTS: Here we show that cholesterol is elevated in malignant ascites and modulates the sensitivity of ovarian cancer cells to CDDP and PAC by upregulating the expression of drug efflux pump proteins, ABCG2 and MDR1, together with upregulation of LXRalpha/beta, the cholesterol receptor. Cholesterol 27-38 ATP binding cassette subfamily B member 1 Homo sapiens 213-217 30526541-10 2018 Cholesterol depletion by methyl beta cyclodextrin (MbetaCD) inhibited malignant ascites-induced chemoresistance to CDDP and upregulation of MDR1 and LXRalpha/beta. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 140-144 30526541-12 2018 CONCLUSIONS: High cholesterol in malignant ascites contributes to poor prognosis in ovarian cancer patients, partly by contributing to multidrug resistance through upregulation of MDR1 via activation of LXRalpha/beta. Cholesterol 18-29 ATP binding cassette subfamily B member 1 Homo sapiens 180-184 28189737-0 2018 The ABCB1 2677G>T/A polymorphism is associated with baseline blood HDL-cholesterol levels in newly diagnosed hyperlipidemic patients. Cholesterol 74-85 ATP binding cassette subfamily B member 1 Homo sapiens 4-9 29353693-10 2018 We also observed the interaction of cholesterol with three distinct areas of the P-gp. Cholesterol 36-47 ATP binding cassette subfamily B member 1 Homo sapiens 81-85 28710789-6 2017 ATR-101 blocked cholesterol efflux and cortisol secretion, suggesting that it inhibited ABCA1, ABCG1, and MDR1 transporters. Cholesterol 16-27 ATP binding cassette subfamily B member 1 Homo sapiens 106-110 28903085-10 2017 We also observed the interaction of cholesterol with three distinct areas of the P-gp. Cholesterol 36-47 ATP binding cassette subfamily B member 1 Homo sapiens 81-85 27152239-13 2016 As well as reverting MDR1-resistance, the inhibitors of P-gp activity induced the CE-HDL/SR-BI pathway by reactivating membrane cholesterol trafficking. Cholesterol 128-139 ATP binding cassette subfamily B member 1 Homo sapiens 21-25 26724201-0 2016 Understanding the accumulation of P-glycoprotein substrates within cells: The effect of cholesterol on membrane partitioning. Cholesterol 88-99 ATP binding cassette subfamily B member 1 Homo sapiens 34-48 26724201-1 2016 The apparent activity of the multidrug transporter P-glycoprotein (P-gp) is enhanced by the presence of cholesterol. Cholesterol 104-115 ATP binding cassette subfamily B member 1 Homo sapiens 51-65 26724201-1 2016 The apparent activity of the multidrug transporter P-glycoprotein (P-gp) is enhanced by the presence of cholesterol. Cholesterol 104-115 ATP binding cassette subfamily B member 1 Homo sapiens 67-71 26724201-2 2016 Whether this is due to the direct effect of cholesterol on the activity of P-gp, its effect on the local concentration of substrate in the membrane, or its effect on the rate of entry of the drug into the cell, is unknown. Cholesterol 44-55 ATP binding cassette subfamily B member 1 Homo sapiens 75-79 26724201-3 2016 In this study, molecular dynamics simulation techniques coupled with potential of mean force calculations have been used to investigate the role of cholesterol in the movement of four P-gp substrates across a POPC bilayer in the presence or absence of 10% cholesterol. Cholesterol 148-159 ATP binding cassette subfamily B member 1 Homo sapiens 184-188 26724201-5 2016 These findings suggest that P-gp substrates may preferentially accumulate in cholesterol-rich regions of the membrane, which may explain its enhanced transport activity. Cholesterol 77-88 ATP binding cassette subfamily B member 1 Homo sapiens 28-32 26704667-0 2016 Cholesterol-dependent conformational changes of P-glycoprotein are detected by the 15D3 monoclonal antibody. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 48-62 26704667-2 2016 Depletion of cell plasma membrane cholesterol by using methyl-beta-cyclodextrin or other chemically modified beta-cyclodextrins decreased the Pgp binding affinity of 15D3 mAb. Cholesterol 34-45 ATP binding cassette subfamily B member 1 Homo sapiens 142-145 26704667-6 2016 Competition studies proposed these two mAbs share overlapping epitopes and can reveal conformational changes of Pgp that correlate (r=0.97) with the cholesterol content of cells. Cholesterol 149-160 ATP binding cassette subfamily B member 1 Homo sapiens 112-115 26704667-9 2016 This study showed that 15D3 mAb bound to a conformation sensitive epitope of Pgp that was responsive to PM cholesterol levels. Cholesterol 107-118 ATP binding cassette subfamily B member 1 Homo sapiens 77-80 27796655-8 2017 Moreover, this trans-enterocytic retrograde cholesterol transport displays some TICE features like modulation by PCSK9 and an ABCB1 inhibitor. Cholesterol 44-55 ATP binding cassette subfamily B member 1 Homo sapiens 126-131 27251372-6 2016 We recently found that the antidiabetic drug vildagliptin stimulates LXR expression leading to increased ABCB1/ABCG1 expression which improves cholesterol efflux from adipocytes. Cholesterol 143-154 ATP binding cassette subfamily B member 1 Homo sapiens 105-110 26026069-9 2015 Microdomain disruption via cholesterol depletion decreased saquinavir"s affinity for P-gp, potentially implicating these structures in the influence of alpha-tocopherol succinate on P-gp. Cholesterol 27-38 ATP binding cassette subfamily B member 1 Homo sapiens 85-89 26026069-9 2015 Microdomain disruption via cholesterol depletion decreased saquinavir"s affinity for P-gp, potentially implicating these structures in the influence of alpha-tocopherol succinate on P-gp. Cholesterol 27-38 ATP binding cassette subfamily B member 1 Homo sapiens 182-186 25603048-5 2015 The ABCB1 has been reported to modulate cellular cholesterol homeostasis. Cholesterol 49-60 ATP binding cassette subfamily B member 1 Homo sapiens 4-9 25640268-6 2015 Still, both ABCB1 and ABCG2 show complex interactions with a variety of lipid molecules, and the transporters are significantly modulated by cholesterol and cholesterol derivatives at the posttranslational level. Cholesterol 141-152 ATP binding cassette subfamily B member 1 Homo sapiens 12-17 25640268-6 2015 Still, both ABCB1 and ABCG2 show complex interactions with a variety of lipid molecules, and the transporters are significantly modulated by cholesterol and cholesterol derivatives at the posttranslational level. Cholesterol 157-168 ATP binding cassette subfamily B member 1 Homo sapiens 12-17 24225025-1 2013 BACKGROUND: The activity of P-glycoprotein (Pgp) and multidrug resistance related protein 1 (MRP1), two membrane transporters involved in multidrug resistance of colon cancer, is increased by high amounts of cholesterol in plasma membrane and detergent resistant membranes (DRMs). Cholesterol 208-219 ATP binding cassette subfamily B member 1 Homo sapiens 28-42 25273894-0 2014 The effect of two novel cholesterol-lowering agents, disodium ascorbyl phytostanol phosphate (DAPP) and nanostructured aluminosilicate (NSAS) on the expression and activity of P-glycoprotein within Caco-2 cells. Cholesterol 24-35 ATP binding cassette subfamily B member 1 Homo sapiens 176-190 25172973-1 2014 The gene product ABCB1 (formerly MDR1 or P-glycoprotein) is hypothesized to be involved in cholesterol cellular trafficking, redistribution and intestinal re-absorption. Cholesterol 91-102 ATP binding cassette subfamily B member 1 Homo sapiens 17-22 25172973-1 2014 The gene product ABCB1 (formerly MDR1 or P-glycoprotein) is hypothesized to be involved in cholesterol cellular trafficking, redistribution and intestinal re-absorption. Cholesterol 91-102 ATP binding cassette subfamily B member 1 Homo sapiens 33-37 24648507-4 2014 Furthermore, several studies showed that P-gp is involved in lipid homeostasis and its activity is regulated by cholesterol. Cholesterol 112-123 ATP binding cassette subfamily B member 1 Homo sapiens 41-45 24505408-11 2014 Disrupting rafts by depleting the membrane of cholesterol increased the functionality of Pgp. Cholesterol 46-57 ATP binding cassette subfamily B member 1 Homo sapiens 89-92 26046259-9 2015 Reduction of cellular cholesterol leads to the accumulation of Pgp substrates, as Pgp requires cholesterol for proper function. Cholesterol 22-33 ATP binding cassette subfamily B member 1 Homo sapiens 63-66 26046259-9 2015 Reduction of cellular cholesterol leads to the accumulation of Pgp substrates, as Pgp requires cholesterol for proper function. Cholesterol 22-33 ATP binding cassette subfamily B member 1 Homo sapiens 82-85 26046259-9 2015 Reduction of cellular cholesterol leads to the accumulation of Pgp substrates, as Pgp requires cholesterol for proper function. Cholesterol 95-106 ATP binding cassette subfamily B member 1 Homo sapiens 63-66 26046259-9 2015 Reduction of cellular cholesterol leads to the accumulation of Pgp substrates, as Pgp requires cholesterol for proper function. Cholesterol 95-106 ATP binding cassette subfamily B member 1 Homo sapiens 82-85 24225025-1 2013 BACKGROUND: The activity of P-glycoprotein (Pgp) and multidrug resistance related protein 1 (MRP1), two membrane transporters involved in multidrug resistance of colon cancer, is increased by high amounts of cholesterol in plasma membrane and detergent resistant membranes (DRMs). Cholesterol 208-219 ATP binding cassette subfamily B member 1 Homo sapiens 44-47 24225025-4 2013 RESULTS: MDR cells, which overexpressed Pgp and MRP1, had a dysregulated cholesterol metabolism, due to the lower expression of ubiquitin E3 ligase Trc8: this produced lower ubiquitination rate of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCoAR), higher cholesterol synthesis, higher cholesterol content in MDR cells. Cholesterol 73-84 ATP binding cassette subfamily B member 1 Homo sapiens 40-43 24225025-4 2013 RESULTS: MDR cells, which overexpressed Pgp and MRP1, had a dysregulated cholesterol metabolism, due to the lower expression of ubiquitin E3 ligase Trc8: this produced lower ubiquitination rate of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCoAR), higher cholesterol synthesis, higher cholesterol content in MDR cells. Cholesterol 263-274 ATP binding cassette subfamily B member 1 Homo sapiens 40-43 23473805-11 2013 Addition of cholesterol attenuated Rp1-induced raft aggregation and MDR-1 redistribution. Cholesterol 12-23 ATP binding cassette subfamily B member 1 Homo sapiens 68-73 23473805-4 2013 MDR-1 is known to be localized in the cholesterol- and sphingolipid-enriched plasma membrane microdomains, known as lipid rafts. Cholesterol 38-49 ATP binding cassette subfamily B member 1 Homo sapiens 0-5 21786699-1 2011 The influence of agents modifying cholesterol in plasma membranes on the functional activity of transporter proteins (P-glycoprotein (P-gp) and the multidrug resistance protein 1 (MRP1)) in human lymphocytes has been studied. Cholesterol 34-45 ATP binding cassette subfamily B member 1 Homo sapiens 118-132 22858002-7 2012 Additionally, we showed that DOPC was partly effluxed via ABCG1, while Chol was partly effluxed via ABCA1 or ABCB1; suggesting that each liposomal component examined in this study was effluxed through different transporters. Cholesterol 71-75 ATP binding cassette subfamily B member 1 Homo sapiens 109-114 22223861-5 2012 These cholesterol-rich microdomains are important, owing to enrichment therein of significant amounts of key transport proteins involved in uptake of cholesterol [SR-B1, ABCA-1, P-glycoprotein (P-gp), sterol carrier binding protein (SCP-2)], FA transport protein (FATP), and glucose transporters 1 and 2 (GLUT1, GLUT2) insulin receptor. Cholesterol 6-17 ATP binding cassette subfamily B member 1 Homo sapiens 178-192 22223861-5 2012 These cholesterol-rich microdomains are important, owing to enrichment therein of significant amounts of key transport proteins involved in uptake of cholesterol [SR-B1, ABCA-1, P-glycoprotein (P-gp), sterol carrier binding protein (SCP-2)], FA transport protein (FATP), and glucose transporters 1 and 2 (GLUT1, GLUT2) insulin receptor. Cholesterol 6-17 ATP binding cassette subfamily B member 1 Homo sapiens 194-198 22223861-5 2012 These cholesterol-rich microdomains are important, owing to enrichment therein of significant amounts of key transport proteins involved in uptake of cholesterol [SR-B1, ABCA-1, P-glycoprotein (P-gp), sterol carrier binding protein (SCP-2)], FA transport protein (FATP), and glucose transporters 1 and 2 (GLUT1, GLUT2) insulin receptor. Cholesterol 150-161 ATP binding cassette subfamily B member 1 Homo sapiens 178-192 22223861-5 2012 These cholesterol-rich microdomains are important, owing to enrichment therein of significant amounts of key transport proteins involved in uptake of cholesterol [SR-B1, ABCA-1, P-glycoprotein (P-gp), sterol carrier binding protein (SCP-2)], FA transport protein (FATP), and glucose transporters 1 and 2 (GLUT1, GLUT2) insulin receptor. Cholesterol 150-161 ATP binding cassette subfamily B member 1 Homo sapiens 194-198 21445698-11 2011 In conclusion, an association between ABCB1 haplotype and plasma fasting LDL cholesterol concentration was found in patients with advanced heart failure. Cholesterol 77-88 ATP binding cassette subfamily B member 1 Homo sapiens 38-43 21786699-1 2011 The influence of agents modifying cholesterol in plasma membranes on the functional activity of transporter proteins (P-glycoprotein (P-gp) and the multidrug resistance protein 1 (MRP1)) in human lymphocytes has been studied. Cholesterol 34-45 ATP binding cassette subfamily B member 1 Homo sapiens 134-138 21786699-2 2011 It was shown that changes in lateral distribution of cholesterol using the polyene antibiotic filipin, which disturb the structure and function of glycolipid microdomains in plasma membranes of lymphocytes lead to a decrease in the transport activity of both P-gp and MRP1. Cholesterol 53-64 ATP binding cassette subfamily B member 1 Homo sapiens 259-263 21786699-2 2011 It was shown that changes in lateral distribution of cholesterol using the polyene antibiotic filipin, which disturb the structure and function of glycolipid microdomains in plasma membranes of lymphocytes lead to a decrease in the transport activity of both P-gp and MRP1. Cholesterol 53-64 ATP binding cassette subfamily B member 1 Homo sapiens 268-272 21786699-4 2011 It was concluded that the transport activity of P-gp and MRP1 depends on the modification of cholesterol in the membranes of human lymphocytes, i.e., is closely associated with the level of cholesterol and its lateral distribution. Cholesterol 93-104 ATP binding cassette subfamily B member 1 Homo sapiens 48-52 21786699-4 2011 It was concluded that the transport activity of P-gp and MRP1 depends on the modification of cholesterol in the membranes of human lymphocytes, i.e., is closely associated with the level of cholesterol and its lateral distribution. Cholesterol 93-104 ATP binding cassette subfamily B member 1 Homo sapiens 57-61 21786699-4 2011 It was concluded that the transport activity of P-gp and MRP1 depends on the modification of cholesterol in the membranes of human lymphocytes, i.e., is closely associated with the level of cholesterol and its lateral distribution. Cholesterol 190-201 ATP binding cassette subfamily B member 1 Homo sapiens 48-52 21786699-4 2011 It was concluded that the transport activity of P-gp and MRP1 depends on the modification of cholesterol in the membranes of human lymphocytes, i.e., is closely associated with the level of cholesterol and its lateral distribution. Cholesterol 190-201 ATP binding cassette subfamily B member 1 Homo sapiens 57-61 20944122-0 2010 Caveolin-1 and doxorubicin-induced P-glycoprotein modulate plasma cholesterol membrane accessibility in erythrolymphoblastic cell line. Cholesterol 66-77 ATP binding cassette subfamily B member 1 Homo sapiens 35-49 20946921-2 2011 Several factors are associated to a high Pgp activity, including the amount of cholesterol in plasma membrane, which is essential to maintain the pump function. Cholesterol 79-90 ATP binding cassette subfamily B member 1 Homo sapiens 41-44 21392722-12 2011 CONCLUSION: In patients treated with atorvastatin, the CC genotype at the C3435T polymorphism in ABCB1 is associated with reduced atorvastatin efficacy independently of cholesterol metabolism. Cholesterol 169-180 ATP binding cassette subfamily B member 1 Homo sapiens 97-102 20944122-1 2010 UNLABELLED: AIM/ BACKGROUND: Various interactions between Caveolae membrane domains, multidrug resistance transporter P-glycoprotein (P-gp) and cholesterol have been suggested. Cholesterol 144-155 ATP binding cassette subfamily B member 1 Homo sapiens 118-132 20944122-1 2010 UNLABELLED: AIM/ BACKGROUND: Various interactions between Caveolae membrane domains, multidrug resistance transporter P-glycoprotein (P-gp) and cholesterol have been suggested. Cholesterol 144-155 ATP binding cassette subfamily B member 1 Homo sapiens 134-138 20944122-2 2010 We tested the assumption that anthracycline-induced P-gp and Caveolin-1 have correlated effects on cholesterol distribution in plasma membrane. Cholesterol 99-110 ATP binding cassette subfamily B member 1 Homo sapiens 52-56 20944122-8 2010 CONCLUSION: Combination of functional P-gp, caveolae presence and lasting effect of anthracycline treatment appear determinant in free membrane cholesterol homeostasis and likely modulate cholesterol membrane order. Cholesterol 144-155 ATP binding cassette subfamily B member 1 Homo sapiens 38-42 20944122-8 2010 CONCLUSION: Combination of functional P-gp, caveolae presence and lasting effect of anthracycline treatment appear determinant in free membrane cholesterol homeostasis and likely modulate cholesterol membrane order. Cholesterol 188-199 ATP binding cassette subfamily B member 1 Homo sapiens 38-42 19891551-0 2009 Common genetic variation in the ABCB1 gene is associated with the cholesterol-lowering effect of simvastatin in males. Cholesterol 66-77 ATP binding cassette subfamily B member 1 Homo sapiens 32-37 20712525-1 2010 AIMS: Genetic variability within the SLCO1B1 and ABCB1 transporter genes has been associated with modification of statin effectiveness in cholesterol management. Cholesterol 138-149 ATP binding cassette subfamily B member 1 Homo sapiens 49-54 20220747-1 2010 Organ transplant recipients who have dyslipidemia related to immunosuppression may benefit from cholesterol-lowering therapy with ezetimibe, a substrate of ABCB1, ABCC2, and OATP1B1. Cholesterol 96-107 ATP binding cassette subfamily B member 1 Homo sapiens 156-161 19802823-2 2010 We studied whether the polymorphism CYP3A4*1B and the polymorphisms C1236T, G2677A/T and C3435T in the ABCB1 gene were associated with a decrease of the prescribed dose or a switch to another cholesterol lowering drug during simvastatin and atorvastatin therapy. Cholesterol 192-203 ATP binding cassette subfamily B member 1 Homo sapiens 103-108 20586189-0 2010 [Cholesterol-modified anti-MDR1 small interfering RNA: uptake and biological activity]. Cholesterol 1-12 ATP binding cassette subfamily B member 1 Homo sapiens 27-31 19891551-1 2009 AIMS: The cholesterol-lowering drug simvastatin is a substrate for P-glycoprotein (P-gp). Cholesterol 10-21 ATP binding cassette subfamily B member 1 Homo sapiens 67-81 19891551-1 2009 AIMS: The cholesterol-lowering drug simvastatin is a substrate for P-glycoprotein (P-gp). Cholesterol 10-21 ATP binding cassette subfamily B member 1 Homo sapiens 83-87 19891551-3 2009 We examined the Rotterdam Study, which is a population-based cohort study of people aged 55 years and older, to see whether the C1236T, G2677T/A and C3435T polymorphisms and haplotypes in the ABCB1 gene are associated with the total cholesterol and low-density lipoprotein cholesterol-lowering effect of simvastatin. Cholesterol 233-244 ATP binding cassette subfamily B member 1 Homo sapiens 192-197 19891551-5 2009 Associations between the ABCB1 gene variants and reductions in cholesterol levels were analyzed. Cholesterol 63-74 ATP binding cassette subfamily B member 1 Homo sapiens 25-30 19891551-7 2009 RESULTS: The three ABCB1 polymorphisms were associated with total cholesterol reduction in the whole population. Cholesterol 66-77 ATP binding cassette subfamily B member 1 Homo sapiens 19-24 19891551-10 2009 CONCLUSION: Male simvastatin users with the ABCB1 1236/2677/3435 TTT and CGT haplotype have larger reductions in total cholesterol and low-density lipoprotein cholesterol compared with the wild-type CGC haplotype. Cholesterol 119-130 ATP binding cassette subfamily B member 1 Homo sapiens 44-49 19651114-5 2009 Two of these transporters are relevant to multidrug resistance in tumor cells (Pgp/ABCB1 and MRP1/ABCC1), while the third (ABCA1) is extensively studied in relation to the reverse cholesterol pathway and cellular cholesterol homeostasis. Cholesterol 180-191 ATP binding cassette subfamily B member 1 Homo sapiens 83-88 19651114-5 2009 Two of these transporters are relevant to multidrug resistance in tumor cells (Pgp/ABCB1 and MRP1/ABCC1), while the third (ABCA1) is extensively studied in relation to the reverse cholesterol pathway and cellular cholesterol homeostasis. Cholesterol 213-224 ATP binding cassette subfamily B member 1 Homo sapiens 83-88 19530968-1 2009 This report focuses on the effects of cholesterol on the expression and function of the ATP-binding cassette (ABCB1, ABCG2 and ABCC2) and solute-linked carrier (SLCO1B1 and SLCO2B1) drug transporters with a particular focus on the potential impact of cholesterol on lipid-lowering drug disposition. Cholesterol 38-49 ATP binding cassette subfamily B member 1 Homo sapiens 110-115 19538007-7 2009 Surface charge of liposomes did not affect the rhodamine retention, whereas the incorporation of cholesterol and polyethyleneglycol-attached lipids was effective in further increasing the rhodamine retention in MCF-7/Pgp cells. Cholesterol 97-108 ATP binding cassette subfamily B member 1 Homo sapiens 217-220 19394420-7 2009 Addition of cholesterol to serum free media abolished this latter effect on ABCB1 mRNA levels. Cholesterol 12-23 ATP binding cassette subfamily B member 1 Homo sapiens 76-81 18837012-5 2009 The MDR1-MDCKII cell membrane has lower ratios of: phospholipid to cholesterol, unsaturated to saturated acyl chains, and phosphatidyl-choline (PC) to sphingomyelin (SM) than brain endothelial cells, making it a poor passive permeability model for BBB. Cholesterol 67-78 ATP binding cassette subfamily B member 1 Homo sapiens 4-8 19530968-1 2009 This report focuses on the effects of cholesterol on the expression and function of the ATP-binding cassette (ABCB1, ABCG2 and ABCC2) and solute-linked carrier (SLCO1B1 and SLCO2B1) drug transporters with a particular focus on the potential impact of cholesterol on lipid-lowering drug disposition. Cholesterol 251-262 ATP binding cassette subfamily B member 1 Homo sapiens 110-115 17955353-3 2007 We compared the functions of drug-transporting and lipid-transporting ABC proteins, and found that (1) ABC proteins, either lipid or drug transporters, have a similar substrate binding site which recognizes PL and cholesterol, or drugs and cholesterol; (2) Cholesterol in membranes binds to various ABC proteins together with PL or drugs, and plays an important role in substrate recognition, especially by ABCB1/MDR1, where cholesterol fills the empty space in the substrate binding site when small drugs bind to it. Cholesterol 214-225 ATP binding cassette subfamily B member 1 Homo sapiens 407-412 18937360-0 2009 Cholesterol-mediated activation of P-glycoprotein: distinct effects on basal and drug-induced ATPase activities. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 35-49 18937360-1 2009 Cholesterol promotes basal and verapamil-induced ATPase activity of P-glycoprotein (P-gp). Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 68-82 18937360-1 2009 Cholesterol promotes basal and verapamil-induced ATPase activity of P-glycoprotein (P-gp). Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 84-88 18937360-3 2009 P-gp was reconstituted in egg-phosphatidylcholine (PhC) liposomes with or without cholesterol, 1,2-dipalmitoyl-phosphatidylcholine (DPPC), alpha-tocopherol (alpha-Toc) or 2,2,5,7,8-pentamethyl-6-chromanol (PMC). Cholesterol 82-93 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 18937360-10 2009 In contrast, verapamil stimulation of P-gp ATPase activity was not only enabled by cholesterol but also by alpha-Toc and DPPC. Cholesterol 83-94 ATP binding cassette subfamily B member 1 Homo sapiens 38-42 18851956-10 2009 In conclusion, ABCB1 and ABCC1 mRNA levels in PBMC are modulated by atorvastatin and ABCB1 G2677T/A polymorphism and ABCB1 baseline expression is related to differences in serum LDL cholesterol and apoB in response to atorvastatin. Cholesterol 182-193 ATP binding cassette subfamily B member 1 Homo sapiens 15-20 18851956-10 2009 In conclusion, ABCB1 and ABCC1 mRNA levels in PBMC are modulated by atorvastatin and ABCB1 G2677T/A polymorphism and ABCB1 baseline expression is related to differences in serum LDL cholesterol and apoB in response to atorvastatin. Cholesterol 182-193 ATP binding cassette subfamily B member 1 Homo sapiens 85-90 18851956-10 2009 In conclusion, ABCB1 and ABCC1 mRNA levels in PBMC are modulated by atorvastatin and ABCB1 G2677T/A polymorphism and ABCB1 baseline expression is related to differences in serum LDL cholesterol and apoB in response to atorvastatin. Cholesterol 182-193 ATP binding cassette subfamily B member 1 Homo sapiens 85-90 19049391-0 2008 Interaction of the P-glycoprotein multidrug efflux pump with cholesterol: effects on ATPase activity, drug binding and transport. Cholesterol 61-72 ATP binding cassette subfamily B member 1 Homo sapiens 19-33 19049391-4 2008 Various interactions between cholesterol and Pgp have been suggested, including a role for the protein in transbilayer movement of cholesterol. Cholesterol 29-40 ATP binding cassette subfamily B member 1 Homo sapiens 45-48 19049391-4 2008 Various interactions between cholesterol and Pgp have been suggested, including a role for the protein in transbilayer movement of cholesterol. Cholesterol 131-142 ATP binding cassette subfamily B member 1 Homo sapiens 45-48 19049391-5 2008 We have characterized several aspects of Pgp-cholesterol interactions, and found that some of the previously reported effects of cholesterol result from inhibition of Pgp ATPase activity by the cholesterol-extracting reagent, methyl-beta-cyclodextrin. Cholesterol 45-56 ATP binding cassette subfamily B member 1 Homo sapiens 41-44 19049391-5 2008 We have characterized several aspects of Pgp-cholesterol interactions, and found that some of the previously reported effects of cholesterol result from inhibition of Pgp ATPase activity by the cholesterol-extracting reagent, methyl-beta-cyclodextrin. Cholesterol 45-56 ATP binding cassette subfamily B member 1 Homo sapiens 167-170 19049391-5 2008 We have characterized several aspects of Pgp-cholesterol interactions, and found that some of the previously reported effects of cholesterol result from inhibition of Pgp ATPase activity by the cholesterol-extracting reagent, methyl-beta-cyclodextrin. Cholesterol 129-140 ATP binding cassette subfamily B member 1 Homo sapiens 41-44 19049391-5 2008 We have characterized several aspects of Pgp-cholesterol interactions, and found that some of the previously reported effects of cholesterol result from inhibition of Pgp ATPase activity by the cholesterol-extracting reagent, methyl-beta-cyclodextrin. Cholesterol 129-140 ATP binding cassette subfamily B member 1 Homo sapiens 167-170 19049391-5 2008 We have characterized several aspects of Pgp-cholesterol interactions, and found that some of the previously reported effects of cholesterol result from inhibition of Pgp ATPase activity by the cholesterol-extracting reagent, methyl-beta-cyclodextrin. Cholesterol 129-140 ATP binding cassette subfamily B member 1 Homo sapiens 41-44 19049391-5 2008 We have characterized several aspects of Pgp-cholesterol interactions, and found that some of the previously reported effects of cholesterol result from inhibition of Pgp ATPase activity by the cholesterol-extracting reagent, methyl-beta-cyclodextrin. Cholesterol 129-140 ATP binding cassette subfamily B member 1 Homo sapiens 167-170 19049391-6 2008 The presence of cholesterol in the bilayer modulated the basal and drug-stimulated ATPase activity of reconstituted Pgp in a modest fashion. Cholesterol 16-27 ATP binding cassette subfamily B member 1 Homo sapiens 116-119 19049391-7 2008 Both the ability of drugs to bind to the protein and the drug transport and phospholipid flippase functions of Pgp were also affected by cholesterol. Cholesterol 137-148 ATP binding cassette subfamily B member 1 Homo sapiens 111-114 19049391-9 2008 Increasing cholesterol content greatly altered the partitioning of hydrophobic drug substrates into the membrane, which may account for some of the observed effects of cholesterol on Pgp-mediated drug transport. Cholesterol 11-22 ATP binding cassette subfamily B member 1 Homo sapiens 183-186 19049391-9 2008 Increasing cholesterol content greatly altered the partitioning of hydrophobic drug substrates into the membrane, which may account for some of the observed effects of cholesterol on Pgp-mediated drug transport. Cholesterol 168-179 ATP binding cassette subfamily B member 1 Homo sapiens 183-186 19049391-11 2008 Cholesterol likely modulates Pgp function via effects on drug-membrane partitioning and changes in the local lipid environment of the protein. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 29-32 18539442-0 2008 P-glycoprotein inhibition by membrane cholesterol modulation. Cholesterol 38-49 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 18539442-2 2008 Pgp molecules are partially localized in TX-100-resistant rafts, and the activity of the transporter is highly sensitive to the presence of cholesterol. Cholesterol 140-151 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 18539442-3 2008 To better understand these relationships, the influence of membrane cholesterol content on Pgp function, as measured via calcein accumulation, was studied in correlation with changes elicited in membrane structure. Cholesterol 68-79 ATP binding cassette subfamily B member 1 Homo sapiens 91-94 19285054-2 2009 Like other ABC transporters, ABCB1 might be implicated in cholesterol homeostasis and ABCB1 polymorphisms which are responsible for drug resistance might affect lipid homeostasis. Cholesterol 58-69 ATP binding cassette subfamily B member 1 Homo sapiens 29-34 18288958-11 2008 Cells transfected with human P-gp esterified more cholesterol. Cholesterol 50-61 ATP binding cassette subfamily B member 1 Homo sapiens 29-33 17955353-3 2007 We compared the functions of drug-transporting and lipid-transporting ABC proteins, and found that (1) ABC proteins, either lipid or drug transporters, have a similar substrate binding site which recognizes PL and cholesterol, or drugs and cholesterol; (2) Cholesterol in membranes binds to various ABC proteins together with PL or drugs, and plays an important role in substrate recognition, especially by ABCB1/MDR1, where cholesterol fills the empty space in the substrate binding site when small drugs bind to it. Cholesterol 214-225 ATP binding cassette subfamily B member 1 Homo sapiens 413-417 17955353-3 2007 We compared the functions of drug-transporting and lipid-transporting ABC proteins, and found that (1) ABC proteins, either lipid or drug transporters, have a similar substrate binding site which recognizes PL and cholesterol, or drugs and cholesterol; (2) Cholesterol in membranes binds to various ABC proteins together with PL or drugs, and plays an important role in substrate recognition, especially by ABCB1/MDR1, where cholesterol fills the empty space in the substrate binding site when small drugs bind to it. Cholesterol 240-251 ATP binding cassette subfamily B member 1 Homo sapiens 407-412 17955353-3 2007 We compared the functions of drug-transporting and lipid-transporting ABC proteins, and found that (1) ABC proteins, either lipid or drug transporters, have a similar substrate binding site which recognizes PL and cholesterol, or drugs and cholesterol; (2) Cholesterol in membranes binds to various ABC proteins together with PL or drugs, and plays an important role in substrate recognition, especially by ABCB1/MDR1, where cholesterol fills the empty space in the substrate binding site when small drugs bind to it. Cholesterol 240-251 ATP binding cassette subfamily B member 1 Homo sapiens 413-417 17955353-3 2007 We compared the functions of drug-transporting and lipid-transporting ABC proteins, and found that (1) ABC proteins, either lipid or drug transporters, have a similar substrate binding site which recognizes PL and cholesterol, or drugs and cholesterol; (2) Cholesterol in membranes binds to various ABC proteins together with PL or drugs, and plays an important role in substrate recognition, especially by ABCB1/MDR1, where cholesterol fills the empty space in the substrate binding site when small drugs bind to it. Cholesterol 240-251 ATP binding cassette subfamily B member 1 Homo sapiens 407-412 17955353-3 2007 We compared the functions of drug-transporting and lipid-transporting ABC proteins, and found that (1) ABC proteins, either lipid or drug transporters, have a similar substrate binding site which recognizes PL and cholesterol, or drugs and cholesterol; (2) Cholesterol in membranes binds to various ABC proteins together with PL or drugs, and plays an important role in substrate recognition, especially by ABCB1/MDR1, where cholesterol fills the empty space in the substrate binding site when small drugs bind to it. Cholesterol 240-251 ATP binding cassette subfamily B member 1 Homo sapiens 413-417 17652262-3 2007 For P-glycoprotein, another member of the ABC transporter family, it is well established that it is at least partly located in cholesterol and sphingolipid-enriched domains of the plasma membrane called "lipid rafts" and that the composition of the membrane lipids may modulate its efflux activity. Cholesterol 127-138 ATP binding cassette subfamily B member 1 Homo sapiens 4-18 17497080-0 2007 P-glycoprotein in proteoliposomes with low residual detergent: the effects of cholesterol. Cholesterol 78-89 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 17497080-1 2007 PURPOSE: There is evidence that cholesterol affects the ATPase and transport functions of P-glycoprotein (P-gp). Cholesterol 32-43 ATP binding cassette subfamily B member 1 Homo sapiens 90-104 17497080-1 2007 PURPOSE: There is evidence that cholesterol affects the ATPase and transport functions of P-glycoprotein (P-gp). Cholesterol 32-43 ATP binding cassette subfamily B member 1 Homo sapiens 106-110 17497080-7 2007 In proteoliposomes containing 20 and 40% cholesterol, respectively, the modulators showed significant P-gp binding and ATPase activation. Cholesterol 41-52 ATP binding cassette subfamily B member 1 Homo sapiens 102-106 17497080-9 2007 CONCLUSIONS: Cholesterol influences P-gp in three ways: (a) it enhances its basal ATPase activity, (b) it renders P-gp sensitive towards the modulators verapamil and progesterone and (c) it affects the modulator concentration at half maximal ATPase activation. Cholesterol 13-24 ATP binding cassette subfamily B member 1 Homo sapiens 36-40 17497080-9 2007 CONCLUSIONS: Cholesterol influences P-gp in three ways: (a) it enhances its basal ATPase activity, (b) it renders P-gp sensitive towards the modulators verapamil and progesterone and (c) it affects the modulator concentration at half maximal ATPase activation. Cholesterol 13-24 ATP binding cassette subfamily B member 1 Homo sapiens 114-118 17901905-0 2007 Reversal of P-glycoprotein-mediated multidrug resistance by cholesterol derived from low density lipoprotein in a vinblastine-resistant human lymphoblastic leukemia cell line. Cholesterol 60-71 ATP binding cassette subfamily B member 1 Homo sapiens 12-26 17901905-2 2007 Studies have shown that the biosynthesis of cholesterol and cholesterol esters interfere with the function of P-gp. Cholesterol 44-55 ATP binding cassette subfamily B member 1 Homo sapiens 110-114 17923246-3 2007 In addition to its role in drug efflux, pgp has been implicated in cellular cholesterol homeostasis. Cholesterol 76-87 ATP binding cassette subfamily B member 1 Homo sapiens 40-43 17923246-4 2007 We investigated the effects of exogenous cholesterol removal on pgp expression and function. Cholesterol 41-52 ATP binding cassette subfamily B member 1 Homo sapiens 64-67 17923246-13 2007 CONCLUSION: LDL cholesterol contributes to pgp expression and chemoresistance in primitive leukemia cells. Cholesterol 16-27 ATP binding cassette subfamily B member 1 Homo sapiens 43-46 17608770-4 2007 MDR2, the amino acid sequence of which has 86% similarity to that of MDR1, excretes phosphatidylcholine and cholesterol in the presence of bile salts. Cholesterol 108-119 ATP binding cassette subfamily B member 1 Homo sapiens 69-73 17653693-1 2007 Several studies have demonstrated that the adenosine triphosphate-binding cassette transporter P-glycoprotein (P-gp) is at least partly located in cholesterol- and sphingolipid-enriched parts of the plasma membrane called "lipid rafts" and that modification of cellular cholesterol content has an impact on the activity of P-gp in vitro and ex vivo. Cholesterol 147-158 ATP binding cassette subfamily B member 1 Homo sapiens 95-109 17653693-1 2007 Several studies have demonstrated that the adenosine triphosphate-binding cassette transporter P-glycoprotein (P-gp) is at least partly located in cholesterol- and sphingolipid-enriched parts of the plasma membrane called "lipid rafts" and that modification of cellular cholesterol content has an impact on the activity of P-gp in vitro and ex vivo. Cholesterol 147-158 ATP binding cassette subfamily B member 1 Homo sapiens 111-115 17653693-1 2007 Several studies have demonstrated that the adenosine triphosphate-binding cassette transporter P-glycoprotein (P-gp) is at least partly located in cholesterol- and sphingolipid-enriched parts of the plasma membrane called "lipid rafts" and that modification of cellular cholesterol content has an impact on the activity of P-gp in vitro and ex vivo. Cholesterol 147-158 ATP binding cassette subfamily B member 1 Homo sapiens 323-327 17653693-1 2007 Several studies have demonstrated that the adenosine triphosphate-binding cassette transporter P-glycoprotein (P-gp) is at least partly located in cholesterol- and sphingolipid-enriched parts of the plasma membrane called "lipid rafts" and that modification of cellular cholesterol content has an impact on the activity of P-gp in vitro and ex vivo. Cholesterol 270-281 ATP binding cassette subfamily B member 1 Homo sapiens 95-109 17653693-1 2007 Several studies have demonstrated that the adenosine triphosphate-binding cassette transporter P-glycoprotein (P-gp) is at least partly located in cholesterol- and sphingolipid-enriched parts of the plasma membrane called "lipid rafts" and that modification of cellular cholesterol content has an impact on the activity of P-gp in vitro and ex vivo. Cholesterol 270-281 ATP binding cassette subfamily B member 1 Homo sapiens 111-115 17653693-3 2007 The aim of our study was therefore to investigate whether differences in individual plasma low-density lipoprotein (LDL) cholesterol levels in humans have an impact on cholesterol content in peripheral blood mononuclear cells (PBMCs) and thereby on individual activity of P-gp. Cholesterol 121-132 ATP binding cassette subfamily B member 1 Homo sapiens 272-276 17608770-6 2007 Cholesterol also binds directly to MDR1 and modulates substrate recognition by MDR1. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 35-39 17608770-6 2007 Cholesterol also binds directly to MDR1 and modulates substrate recognition by MDR1. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 79-83 17608770-7 2007 Cholesterol may fill the empty space of the drug-binding site and aid the recognition of small drugs, and facilitates the ability of MDR1 to recognize compounds with various structures and molecular weights. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 133-137 17336270-3 2007 Recent works have suggested that P-glycoprotein is associated to cholesterol and sphingolipid-rich membrane microdomains and that cholesterol upregulates its ATPase and drug transport activities. Cholesterol 65-76 ATP binding cassette subfamily B member 1 Homo sapiens 33-47 17336270-3 2007 Recent works have suggested that P-glycoprotein is associated to cholesterol and sphingolipid-rich membrane microdomains and that cholesterol upregulates its ATPase and drug transport activities. Cholesterol 130-141 ATP binding cassette subfamily B member 1 Homo sapiens 33-47 17336270-4 2007 Confocal microscopy experiments and Triton X-100 extraction of detergent-resistant membranes provide evidence that MRP1 is not located in raft-like structures and that its activity is downregulated by cholesterol. Cholesterol 201-212 ATP binding cassette subfamily B member 1 Homo sapiens 115-119 17029589-10 2007 Paclitaxel-stimulated ATPase activity of MDR1 is enhanced in the presence of stigmasterol, sitosterol and campesterol, as well as cholesterol, but not ergosterol. Cholesterol 130-141 ATP binding cassette subfamily B member 1 Homo sapiens 41-45 17223079-4 2007 Pgp was not liberated from the plasma membrane during cholesterol modulation and functional inhibition of Pgp was related to varying cholesterol levels in the plasma membrane. Cholesterol 133-144 ATP binding cassette subfamily B member 1 Homo sapiens 106-109 17029589-0 2007 Modulation of drug-stimulated ATPase activity of human MDR1/P-glycoprotein by cholesterol. Cholesterol 78-89 ATP binding cassette subfamily B member 1 Homo sapiens 55-59 17029589-0 2007 Modulation of drug-stimulated ATPase activity of human MDR1/P-glycoprotein by cholesterol. Cholesterol 78-89 ATP binding cassette subfamily B member 1 Homo sapiens 60-74 17223079-8 2007 In cholesterol-depleted cell membranes a shift in the Pgp localisation to detergent soluble fractions was observed. Cholesterol 3-14 ATP binding cassette subfamily B member 1 Homo sapiens 54-57 17029589-11 2007 These results suggest that the drug-binding site of MDR1 may best fit drugs with a molecular mass of between 800 and 900 Da, and that cholesterol may support the recognition of smaller drugs by adjusting the drug-binding site and play an important role in the function of MDR1. Cholesterol 134-145 ATP binding cassette subfamily B member 1 Homo sapiens 272-276 17029589-2 2007 It is suggested that drugs bind to MDR1 directly from the lipid bilayer and that cholesterol in the bilayer also interacts with MDR1. Cholesterol 81-92 ATP binding cassette subfamily B member 1 Homo sapiens 35-39 17029589-2 2007 It is suggested that drugs bind to MDR1 directly from the lipid bilayer and that cholesterol in the bilayer also interacts with MDR1. Cholesterol 81-92 ATP binding cassette subfamily B member 1 Homo sapiens 128-132 17029589-3 2007 However, the effects of cholesterol on drug-MDR1 interactions are still unclear. Cholesterol 24-35 ATP binding cassette subfamily B member 1 Homo sapiens 44-48 15308763-0 2004 Modulation of cellular cholesterol alters P-glycoprotein activity in multidrug-resistant cells. Cholesterol 23-34 ATP binding cassette subfamily B member 1 Homo sapiens 42-56 17029589-6 2007 Cholesterol directly binds to purified MDR1 in a detergent soluble form and the effects of cholesterol on drug-stimulated ATPase activity differ from one drug to another. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 39-43 17029589-6 2007 Cholesterol directly binds to purified MDR1 in a detergent soluble form and the effects of cholesterol on drug-stimulated ATPase activity differ from one drug to another. Cholesterol 91-102 ATP binding cassette subfamily B member 1 Homo sapiens 39-43 16138223-0 2005 High baseline serum total and LDL cholesterol levels are associated with MDR1 haplotypes in Brazilian hypercholesterolemic individuals of European descent. Cholesterol 34-45 ATP binding cassette subfamily B member 1 Homo sapiens 73-77 16138223-10 2005 These data indicate that MDR1 polymorphism may have an important contribution to the control of basal serum cholesterol levels in Brazilian hypercholesterolemic individuals of European descent. Cholesterol 108-119 ATP binding cassette subfamily B member 1 Homo sapiens 25-29 15766280-0 2005 Control of P-glycoprotein activity by membrane cholesterol amounts and their relation to multidrug resistance in human CEM leukemia cells. Cholesterol 47-58 ATP binding cassette subfamily B member 1 Homo sapiens 11-25 15766280-2 2005 We observed that in human CEM acute lymphoblastic leukemia cells expressing various degrees of chemoresistance and where P-gp was the sole MDR-related ABC transporter detected, the amount of esterified cholesterol increased linearly with the level of resistance to vinblastine while the amounts of total and free cholesterol increased in a nonlinear way. Cholesterol 202-213 ATP binding cassette subfamily B member 1 Homo sapiens 121-125 15766280-2 2005 We observed that in human CEM acute lymphoblastic leukemia cells expressing various degrees of chemoresistance and where P-gp was the sole MDR-related ABC transporter detected, the amount of esterified cholesterol increased linearly with the level of resistance to vinblastine while the amounts of total and free cholesterol increased in a nonlinear way. Cholesterol 313-324 ATP binding cassette subfamily B member 1 Homo sapiens 121-125 15766280-3 2005 Membrane cholesterol controlled the ATPase activity of P-gp in a linear manner, whereas the P-gp-induced daunomycin efflux decreased nonlinearly with the depletion of membrane cholesterol. Cholesterol 9-20 ATP binding cassette subfamily B member 1 Homo sapiens 55-59 15766280-3 2005 Membrane cholesterol controlled the ATPase activity of P-gp in a linear manner, whereas the P-gp-induced daunomycin efflux decreased nonlinearly with the depletion of membrane cholesterol. Cholesterol 176-187 ATP binding cassette subfamily B member 1 Homo sapiens 92-96 15766280-4 2005 All these elements suggest that cholesterol controls both the ATPase and the drug efflux activities of P-gp. Cholesterol 32-43 ATP binding cassette subfamily B member 1 Homo sapiens 103-107 15766280-5 2005 In addition, in CEM cell lines that expressed increasing levels of elevated chemoresistance, the amount of P-gp increases to a plateau value of 40% of the total membrane proteins and remained unvaried while the amount of membrane cholesterol increased with the elevation of the MDR level, strongly suggesting that cholesterol may be directly involved in the typical MDR phenotype. Cholesterol 314-325 ATP binding cassette subfamily B member 1 Homo sapiens 107-111 15766280-6 2005 Finally, we showed that the decreased daunomycin efflux by P-gp due to the partial depletion of membrane cholesterol was responsible for the efficient chemosensitization of resistant CEM cells, which could be totally reversed after cholesterol repletion. Cholesterol 105-116 ATP binding cassette subfamily B member 1 Homo sapiens 59-63 15766280-6 2005 Finally, we showed that the decreased daunomycin efflux by P-gp due to the partial depletion of membrane cholesterol was responsible for the efficient chemosensitization of resistant CEM cells, which could be totally reversed after cholesterol repletion. Cholesterol 232-243 ATP binding cassette subfamily B member 1 Homo sapiens 59-63 15718846-0 2005 The efavirenz-induced increase in HDL-cholesterol is influenced by the multidrug resistance gene 1 C3435T polymorphism. Cholesterol 38-49 ATP binding cassette subfamily B member 1 Homo sapiens 71-98 16996216-7 2006 Correlation was found between ABCB1 mRNA levels and creatine kinase (r=0.30; p=0.014) and total cholesterol (r=-0.31; p=0.010). Cholesterol 96-107 ATP binding cassette subfamily B member 1 Homo sapiens 30-35 16996216-10 2006 ABCB1 plays a role in cellular protection as well as in secretion and/or disposition, therefore, inhibition of ABCB1 synthesis may increase the atorvastatin efficacy, leading to a more pronounced reduction of plasma cholesterol. Cholesterol 216-227 ATP binding cassette subfamily B member 1 Homo sapiens 0-5 16996216-10 2006 ABCB1 plays a role in cellular protection as well as in secretion and/or disposition, therefore, inhibition of ABCB1 synthesis may increase the atorvastatin efficacy, leading to a more pronounced reduction of plasma cholesterol. Cholesterol 216-227 ATP binding cassette subfamily B member 1 Homo sapiens 111-116 16885132-0 2006 Does p-glycoprotein play a role in gastrointestinal absorption and cellular transport of dietary cholesterol? Cholesterol 97-108 ATP binding cassette subfamily B member 1 Homo sapiens 5-19 16885132-1 2006 This commentary discusses the potential role of p-glycoprotein (Pgp) on the gastrointestinal absorption and cellular transport of dietary cholesterol. Cholesterol 138-149 ATP binding cassette subfamily B member 1 Homo sapiens 48-62 16885132-1 2006 This commentary discusses the potential role of p-glycoprotein (Pgp) on the gastrointestinal absorption and cellular transport of dietary cholesterol. Cholesterol 138-149 ATP binding cassette subfamily B member 1 Homo sapiens 64-67 16513445-0 2006 Intestinal expression of P-glycoprotein (ABCB1), multidrug resistance associated protein 2 (ABCC2), and uridine diphosphate-glucuronosyltransferase 1A1 predicts the disposition and modulates the effects of the cholesterol absorption inhibitor ezetimibe in humans. Cholesterol 210-221 ATP binding cassette subfamily B member 1 Homo sapiens 25-39 16513445-0 2006 Intestinal expression of P-glycoprotein (ABCB1), multidrug resistance associated protein 2 (ABCC2), and uridine diphosphate-glucuronosyltransferase 1A1 predicts the disposition and modulates the effects of the cholesterol absorption inhibitor ezetimibe in humans. Cholesterol 210-221 ATP binding cassette subfamily B member 1 Homo sapiens 41-46 16176266-2 2005 Pgp can also operate as a phospholipid and glycosphingolipid flippase, and has been functionally linked to cholesterol, suggesting that it might be associated with sphingolipid-cholesterol microdomains in cell membranes. Cholesterol 107-118 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 16176266-2 2005 Pgp can also operate as a phospholipid and glycosphingolipid flippase, and has been functionally linked to cholesterol, suggesting that it might be associated with sphingolipid-cholesterol microdomains in cell membranes. Cholesterol 177-188 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 16176266-8 2005 We found that the buoyant density of the Brij-96-based Pgp-containing microdomains was sensitive to cholesterol removal by methyl-beta-cyclodextrin. Cholesterol 100-111 ATP binding cassette subfamily B member 1 Homo sapiens 55-58 15693753-5 2005 A 40% cholesterol depletion of DRM caused the loss of 52% of the P-gp ATPase activity. Cholesterol 6-17 ATP binding cassette subfamily B member 1 Homo sapiens 65-69 15693753-6 2005 Cholesterol repletion allowed recovery of the initial P-gp ATPase activity. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 54-58 15693753-7 2005 In contrast, in the solubilized-membrane-containing fractions, cholesterol depletion and repletion had no effect on the P-gp ATPase activity whereas up to 100% saturation with cholesterol induced a 58% increased P-gp ATPase activity, while no significant modification was observed for the DRM-enriched fraction. Cholesterol 176-187 ATP binding cassette subfamily B member 1 Homo sapiens 212-216 15693753-8 2005 DRMs were analysed by atomic force microscopy: 40-60% cholesterol depletion was necessary to remove P-gp from DRMs. Cholesterol 54-65 ATP binding cassette subfamily B member 1 Homo sapiens 100-104 15693753-9 2005 In conclusion, P-gp in DRMs appears to contain closely surrounding cholesterol that can stimulate P-gp ATPase activity to its optimal value, whereas cholesterol in the second population seems deprived of this function. Cholesterol 67-78 ATP binding cassette subfamily B member 1 Homo sapiens 15-19 15693753-9 2005 In conclusion, P-gp in DRMs appears to contain closely surrounding cholesterol that can stimulate P-gp ATPase activity to its optimal value, whereas cholesterol in the second population seems deprived of this function. Cholesterol 67-78 ATP binding cassette subfamily B member 1 Homo sapiens 98-102 15308763-4 2004 In L-MDR1 overexpressing human P-glycoprotein, cholesterol depletion removed P-glycoprotein from the raft membranes into non-DRM fractions, whereas repletion fully reconstituted raft localization. Cholesterol 47-58 ATP binding cassette subfamily B member 1 Homo sapiens 5-9 15308763-4 2004 In L-MDR1 overexpressing human P-glycoprotein, cholesterol depletion removed P-glycoprotein from the raft membranes into non-DRM fractions, whereas repletion fully reconstituted raft localization. Cholesterol 47-58 ATP binding cassette subfamily B member 1 Homo sapiens 31-45 15308763-4 2004 In L-MDR1 overexpressing human P-glycoprotein, cholesterol depletion removed P-glycoprotein from the raft membranes into non-DRM fractions, whereas repletion fully reconstituted raft localization. Cholesterol 47-58 ATP binding cassette subfamily B member 1 Homo sapiens 77-91 15308763-6 2004 Cholesterol depletion reduced P-glycoprotein function in L-MDR1 cells resulting in intracellular substrate accumulation (159% +/- 43, p < 0.001; control = 100%). Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 30-44 15308763-6 2004 Cholesterol depletion reduced P-glycoprotein function in L-MDR1 cells resulting in intracellular substrate accumulation (159% +/- 43, p < 0.001; control = 100%). Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 59-63 15308763-8 2004 Addition of surplus cholesterol (saturation) even enhanced drug efflux in L-MDR1 cells, leading to reduced intracellular accumulation of BODIPY-verapamil (69% +/- 10, p < 0.001). Cholesterol 20-31 ATP binding cassette subfamily B member 1 Homo sapiens 76-80 15308763-10 2004 These results demonstrate that cholesterol alterations influence P-glycoprotein localization and function, which might contribute to the large interindividual variability of P-glycoprotein activity known from in vivo studies. Cholesterol 31-42 ATP binding cassette subfamily B member 1 Homo sapiens 65-79 15308763-10 2004 These results demonstrate that cholesterol alterations influence P-glycoprotein localization and function, which might contribute to the large interindividual variability of P-glycoprotein activity known from in vivo studies. Cholesterol 31-42 ATP binding cassette subfamily B member 1 Homo sapiens 174-188 12145328-0 2002 The multidrug transporter, P-glycoprotein, actively mediates cholesterol redistribution in the cell membrane. Cholesterol 61-72 ATP binding cassette subfamily B member 1 Homo sapiens 27-41 15033457-0 2004 Cholesterol modulates P-glycoprotein activity in human peripheral blood mononuclear cells. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 22-36 15033457-2 2004 P-gp is known to be located in membrane microdomains, whose structure and function are susceptible to cholesterol alterations. Cholesterol 102-113 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 15033457-3 2004 This study evaluated the effect of cholesterol alteration in human PBMCs on P-gp activity. Cholesterol 35-46 ATP binding cassette subfamily B member 1 Homo sapiens 76-80 15033457-6 2004 These data demonstrate that elevated cellular cholesterol levels can markedly increase P-gp activity in human PBMCs. Cholesterol 46-57 ATP binding cassette subfamily B member 1 Homo sapiens 87-91 15139519-0 2004 Contribution of cholesterol and phospholipids to inhibitory effect of dimethyl-beta-cyclodextrin on efflux function of P-glycoprotein and multidrug resistance-associated protein 2 in vinblastine-resistant Caco-2 cell monolayers. Cholesterol 16-27 ATP binding cassette subfamily B member 1 Homo sapiens 119-133 12925201-9 2003 These findings support the notion that ABCB1 and ABCG1 are potentially implicated in cholesterol transport, whereas ABCC1, ABCC3, and ABCC4 are candidate regulators of the translocation of sulfated lipids during stratum corneum keratinization. Cholesterol 85-96 ATP binding cassette subfamily B member 1 Homo sapiens 39-44 12586368-0 2003 Involvement of cholesterol in the inhibitory effect of dimethyl-beta-cyclodextrin on P-glycoprotein and MRP2 function in Caco-2 cells. Cholesterol 15-26 ATP binding cassette subfamily B member 1 Homo sapiens 85-99 15001473-4 2004 In the absence of treatment with anti-CD19, 40% of P-gp molecules expressed by Namalwa/MDR1 cells reside in the low-density lipid (ie, cholesterol-rich) microdomains (lipid rafts). Cholesterol 135-146 ATP binding cassette subfamily B member 1 Homo sapiens 51-55 12586368-5 2003 Therefore, these results suggest that the inhibitory effect of DM-beta-CyD on P-gp and MRP2 function, at least in part, could be attributed to the release of these transporters from the apical membranes into the medium as secondary effects through cholesterol-depletion in caveolae after treatment of Caco-2 cell monolayers with DM-beta-CyD. Cholesterol 248-259 ATP binding cassette subfamily B member 1 Homo sapiens 78-82 12145328-6 2002 Finally, this P-gp-mediated cholesterol redistribution in the cell membrane makes it likely that P-gp contributes in stabilizing the cholesterol-rich microdomains, rafts and caveolae, and that it is involved in the regulation of cholesterol trafficking in cells. Cholesterol 133-144 ATP binding cassette subfamily B member 1 Homo sapiens 97-101 12145328-6 2002 Finally, this P-gp-mediated cholesterol redistribution in the cell membrane makes it likely that P-gp contributes in stabilizing the cholesterol-rich microdomains, rafts and caveolae, and that it is involved in the regulation of cholesterol trafficking in cells. Cholesterol 133-144 ATP binding cassette subfamily B member 1 Homo sapiens 14-18 12145328-6 2002 Finally, this P-gp-mediated cholesterol redistribution in the cell membrane makes it likely that P-gp contributes in stabilizing the cholesterol-rich microdomains, rafts and caveolae, and that it is involved in the regulation of cholesterol trafficking in cells. Cholesterol 133-144 ATP binding cassette subfamily B member 1 Homo sapiens 97-101 12145328-5 2002 These data are highly evocative of a coupling between the basal ATPase activity of P-gp and its intramembrane cholesterol-redistribution function, and they are fully consistent with the possibility that P-gp may actively translocate cholesterol in the membrane. Cholesterol 110-121 ATP binding cassette subfamily B member 1 Homo sapiens 83-87 12145328-5 2002 These data are highly evocative of a coupling between the basal ATPase activity of P-gp and its intramembrane cholesterol-redistribution function, and they are fully consistent with the possibility that P-gp may actively translocate cholesterol in the membrane. Cholesterol 110-121 ATP binding cassette subfamily B member 1 Homo sapiens 203-207 12145328-5 2002 These data are highly evocative of a coupling between the basal ATPase activity of P-gp and its intramembrane cholesterol-redistribution function, and they are fully consistent with the possibility that P-gp may actively translocate cholesterol in the membrane. Cholesterol 233-244 ATP binding cassette subfamily B member 1 Homo sapiens 83-87 12145328-5 2002 These data are highly evocative of a coupling between the basal ATPase activity of P-gp and its intramembrane cholesterol-redistribution function, and they are fully consistent with the possibility that P-gp may actively translocate cholesterol in the membrane. Cholesterol 233-244 ATP binding cassette subfamily B member 1 Homo sapiens 203-207 12145328-6 2002 Finally, this P-gp-mediated cholesterol redistribution in the cell membrane makes it likely that P-gp contributes in stabilizing the cholesterol-rich microdomains, rafts and caveolae, and that it is involved in the regulation of cholesterol trafficking in cells. Cholesterol 28-39 ATP binding cassette subfamily B member 1 Homo sapiens 14-18 12145328-6 2002 Finally, this P-gp-mediated cholesterol redistribution in the cell membrane makes it likely that P-gp contributes in stabilizing the cholesterol-rich microdomains, rafts and caveolae, and that it is involved in the regulation of cholesterol trafficking in cells. Cholesterol 28-39 ATP binding cassette subfamily B member 1 Homo sapiens 97-101 12145328-6 2002 Finally, this P-gp-mediated cholesterol redistribution in the cell membrane makes it likely that P-gp contributes in stabilizing the cholesterol-rich microdomains, rafts and caveolae, and that it is involved in the regulation of cholesterol trafficking in cells. Cholesterol 133-144 ATP binding cassette subfamily B member 1 Homo sapiens 14-18 11118294-1 2000 The aim of the present paper is to reinvestigate the role of multidrug resistance P-glycoprotein MDR1 and MDR-associated protein (MRP1) in cholesterol esterification using well-characterized inhibitors. Cholesterol 139-150 ATP binding cassette subfamily B member 1 Homo sapiens 97-101 12110376-2 2002 Recent studies suggest that Pgp is also responsible for the intracellular transport of cholesterol from the plasma membrane to the endoplasmic reticulum. Cholesterol 87-98 ATP binding cassette subfamily B member 1 Homo sapiens 28-31 11718296-0 2001 The importance of cholesterol in maintenance of P-glycoprotein activity and its membrane perturbing influence. Cholesterol 18-29 ATP binding cassette subfamily B member 1 Homo sapiens 48-62 11718296-8 2001 Physiological concentrations of cholesterol modified P-gp function and the degree to which it perturbed bilayer organization. Cholesterol 32-43 ATP binding cassette subfamily B member 1 Homo sapiens 53-57 11718296-9 2001 The basal ATPase activity of P-gp was increased in a dose-dependent fashion by the incorporation of cholesterol in PC:PE liposomes. Cholesterol 100-111 ATP binding cassette subfamily B member 1 Homo sapiens 29-33 11718296-13 2001 Cholesterol imparted "stability" to this perturbation of bilayer organization by P-gp and moreover this led to altered protein function. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 81-85 11461099-1 2001 OBJECTIVES: a positive correlation between cholesterol esterification, acyl-CoA:cholesterol acyltransferase (ACAT), multidrug resistance (MDR1) gene expression and atherosclerotic lesions has been shown in human arteries. Cholesterol 43-54 ATP binding cassette subfamily B member 1 Homo sapiens 138-142 11027568-0 2000 Cholesterol interaction with the daunorubicin binding site of P-glycoprotein. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 62-76 11027568-8 2000 Suggesting that cholesterol directly interacts with the substrate binding site of P-gp, these results are consistent with cholesterol being transported by MDR1 P-gp. Cholesterol 122-133 ATP binding cassette subfamily B member 1 Homo sapiens 82-86 11027568-8 2000 Suggesting that cholesterol directly interacts with the substrate binding site of P-gp, these results are consistent with cholesterol being transported by MDR1 P-gp. Cholesterol 122-133 ATP binding cassette subfamily B member 1 Homo sapiens 155-159 11027568-8 2000 Suggesting that cholesterol directly interacts with the substrate binding site of P-gp, these results are consistent with cholesterol being transported by MDR1 P-gp. Cholesterol 122-133 ATP binding cassette subfamily B member 1 Homo sapiens 160-164 11027568-3 2000 Recent observations have suggested that, under certain settings, the ABC transporter P-glycoprotein (P-gp) performs a direct role in cholesterol disposition. Cholesterol 133-144 ATP binding cassette subfamily B member 1 Homo sapiens 85-99 11027568-3 2000 Recent observations have suggested that, under certain settings, the ABC transporter P-glycoprotein (P-gp) performs a direct role in cholesterol disposition. Cholesterol 133-144 ATP binding cassette subfamily B member 1 Homo sapiens 101-105 10869171-0 2000 Effects of cholesterol and enantiomeric cholesterol on P-glycoprotein localization and function in low-density membrane domains. Cholesterol 11-22 ATP binding cassette subfamily B member 1 Homo sapiens 55-69 10869171-10 2000 These data show that Pgp preferentially localizes to low-density, cholesterol-enriched membrane domains, but acute depletion of cholesterol impacts Pgp-mediated drug transport in a substrate- and cell-type-specific manner. Cholesterol 128-139 ATP binding cassette subfamily B member 1 Homo sapiens 148-151 10869171-0 2000 Effects of cholesterol and enantiomeric cholesterol on P-glycoprotein localization and function in low-density membrane domains. Cholesterol 40-51 ATP binding cassette subfamily B member 1 Homo sapiens 55-69 11027568-6 2000 In a NIH-G185 cell line presenting an overexpressed amount of the human transporter P-gp, cholesterol caused dramatic inhibition of daunorubicin transport with an IC(50) of about 8 microM yet had no effect on the parent cell line nor rhodamine 123 transport. Cholesterol 90-101 ATP binding cassette subfamily B member 1 Homo sapiens 84-88 10869171-1 2000 Multidrug resistance P-glycoprotein (Pgp) has been reported to localize in low-density, cholesterol-enriched membranes. Cholesterol 88-99 ATP binding cassette subfamily B member 1 Homo sapiens 21-35 10869171-1 2000 Multidrug resistance P-glycoprotein (Pgp) has been reported to localize in low-density, cholesterol-enriched membranes. Cholesterol 88-99 ATP binding cassette subfamily B member 1 Homo sapiens 37-40 10869171-5 2000 Removing cholesterol from cells with beta-methylcyclodextrin (CD), a sterol acceptor molecule, shifted fractions that contained Pgp from low toward high density, and this effect was reversed to a similar extent by restoring sterols with either cholesterol or enantiomeric cholesterol. Cholesterol 9-20 ATP binding cassette subfamily B member 1 Homo sapiens 128-131 11027568-7 2000 Additionally, using the ATP-hydrolysis assay, we showed that cholesterol increases P-gp-mediated ATP hydrolysis by approximately 1.6-fold with a K(s) of 5 microM. Cholesterol 61-72 ATP binding cassette subfamily B member 1 Homo sapiens 83-87 10869171-8 2000 Cell-type-specific effects of cholesterol content on function of human Pgp were detected by use of daunomycin, another substrate for Pgp, although efficacy of inhibitors remained independent of cholesterol. Cholesterol 30-41 ATP binding cassette subfamily B member 1 Homo sapiens 71-74 10869171-8 2000 Cell-type-specific effects of cholesterol content on function of human Pgp were detected by use of daunomycin, another substrate for Pgp, although efficacy of inhibitors remained independent of cholesterol. Cholesterol 30-41 ATP binding cassette subfamily B member 1 Homo sapiens 133-136 11027568-8 2000 Suggesting that cholesterol directly interacts with the substrate binding site of P-gp, these results are consistent with cholesterol being transported by MDR1 P-gp. Cholesterol 16-27 ATP binding cassette subfamily B member 1 Homo sapiens 82-86 10869171-8 2000 Cell-type-specific effects of cholesterol content on function of human Pgp were detected by use of daunomycin, another substrate for Pgp, although efficacy of inhibitors remained independent of cholesterol. Cholesterol 194-205 ATP binding cassette subfamily B member 1 Homo sapiens 71-74 10869171-9 2000 Conversely, both function and inhibition of hamster Pgp as measured with Tc-Sestamibi and daunomycin were in part dependent on normal cell content of cholesterol. Cholesterol 150-161 ATP binding cassette subfamily B member 1 Homo sapiens 52-55 11027568-8 2000 Suggesting that cholesterol directly interacts with the substrate binding site of P-gp, these results are consistent with cholesterol being transported by MDR1 P-gp. Cholesterol 16-27 ATP binding cassette subfamily B member 1 Homo sapiens 155-159 10869171-10 2000 These data show that Pgp preferentially localizes to low-density, cholesterol-enriched membrane domains, but acute depletion of cholesterol impacts Pgp-mediated drug transport in a substrate- and cell-type-specific manner. Cholesterol 66-77 ATP binding cassette subfamily B member 1 Homo sapiens 21-24 11027568-8 2000 Suggesting that cholesterol directly interacts with the substrate binding site of P-gp, these results are consistent with cholesterol being transported by MDR1 P-gp. Cholesterol 16-27 ATP binding cassette subfamily B member 1 Homo sapiens 160-164 10631102-0 2000 Overexpression of MDR1 in an intestinal cell line results in increased cholesterol uptake from micelles. Cholesterol 71-82 ATP binding cassette subfamily B member 1 Homo sapiens 18-22 10811443-3 2000 The question of what, if anything, P-gp does when not effluxing drugs has been raised by recent reports indicating that P-gp may regulate apoptosis, chloride channel activity, cholesterol metabolism and immune cell function. Cholesterol 176-187 ATP binding cassette subfamily B member 1 Homo sapiens 35-39 10811443-3 2000 The question of what, if anything, P-gp does when not effluxing drugs has been raised by recent reports indicating that P-gp may regulate apoptosis, chloride channel activity, cholesterol metabolism and immune cell function. Cholesterol 176-187 ATP binding cassette subfamily B member 1 Homo sapiens 120-124 10631102-3 2000 Several studies using compounds known to act as MDR1 inhibitors have suggested that MDR1 may be involved in the transport of cholesterol from the plasma membrane to the endoplasmic reticulum where it is esterified. Cholesterol 125-136 ATP binding cassette subfamily B member 1 Homo sapiens 48-52 10631102-3 2000 Several studies using compounds known to act as MDR1 inhibitors have suggested that MDR1 may be involved in the transport of cholesterol from the plasma membrane to the endoplasmic reticulum where it is esterified. Cholesterol 125-136 ATP binding cassette subfamily B member 1 Homo sapiens 84-88 10631102-5 2000 MDR1-transfected cells exhibited increased expression of MDR1 protein, reduced accumulation of vinblastine and increased uptake of [(3)H]cholesterol from cholesterol/monolein/taurocholate micelles. Cholesterol 137-148 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 10631102-6 2000 These studies provide the first direct evidence that the level of MDR1 expression in intestinal cells can influence the amount of cholesterol taken up by those cells. Cholesterol 130-141 ATP binding cassette subfamily B member 1 Homo sapiens 66-70 10474039-3 1999 In this study, lipid content and the expression of the genes involved in cholesterol metabolism such as hydroxy-methylglutaryl coenzyme A reductase (HMGCoA-R), low-density lipoprotein receptor (LDL-R), ACAT and MDR1 have been investigated in control and atherosclerotic arteries. Cholesterol 73-84 ATP binding cassette subfamily B member 1 Homo sapiens 211-215 10474039-1 1999 Recent studies have shown that a membrane p-glycoprotein, encoded by MDR1 gene, is involved in the transport of free cholesterol from the plasma membrane to endoplasmic reticulum, the site of cholesterol esterification by acyl-CoA:cholesterol acyltransferase (ACAT). Cholesterol 117-128 ATP binding cassette subfamily B member 1 Homo sapiens 42-56 10474039-1 1999 Recent studies have shown that a membrane p-glycoprotein, encoded by MDR1 gene, is involved in the transport of free cholesterol from the plasma membrane to endoplasmic reticulum, the site of cholesterol esterification by acyl-CoA:cholesterol acyltransferase (ACAT). Cholesterol 117-128 ATP binding cassette subfamily B member 1 Homo sapiens 69-73 10474039-1 1999 Recent studies have shown that a membrane p-glycoprotein, encoded by MDR1 gene, is involved in the transport of free cholesterol from the plasma membrane to endoplasmic reticulum, the site of cholesterol esterification by acyl-CoA:cholesterol acyltransferase (ACAT). Cholesterol 192-203 ATP binding cassette subfamily B member 1 Homo sapiens 42-56 10474039-1 1999 Recent studies have shown that a membrane p-glycoprotein, encoded by MDR1 gene, is involved in the transport of free cholesterol from the plasma membrane to endoplasmic reticulum, the site of cholesterol esterification by acyl-CoA:cholesterol acyltransferase (ACAT). Cholesterol 192-203 ATP binding cassette subfamily B member 1 Homo sapiens 69-73 10066752-0 1999 Multidrug resistance (MDR1) P-glycoprotein enhances esterification of plasma membrane cholesterol. Cholesterol 86-97 ATP binding cassette subfamily B member 1 Homo sapiens 22-26 10066752-0 1999 Multidrug resistance (MDR1) P-glycoprotein enhances esterification of plasma membrane cholesterol. Cholesterol 86-97 ATP binding cassette subfamily B member 1 Homo sapiens 28-42 10066752-4 1999 Compared with parental NIH 3T3 fibroblasts, cells transfected with human multidrug resistance (MDR1) Pgp esterified more cholesterol both without and with sphingomyelinase. Cholesterol 121-132 ATP binding cassette subfamily B member 1 Homo sapiens 95-99 10371303-5 1999 In this study, high levels of cholesterol esterification and of expression of ACAT gene were also associated with a markedly increased expression of multidrug resistance (MDR1) gene, suggesting that MDR1 activity might contribute to regulate the rate of cell growth and division by modulating intracellular cholesterol ester levels. Cholesterol 30-41 ATP binding cassette subfamily B member 1 Homo sapiens 171-175 10371303-5 1999 In this study, high levels of cholesterol esterification and of expression of ACAT gene were also associated with a markedly increased expression of multidrug resistance (MDR1) gene, suggesting that MDR1 activity might contribute to regulate the rate of cell growth and division by modulating intracellular cholesterol ester levels. Cholesterol 30-41 ATP binding cassette subfamily B member 1 Homo sapiens 199-203 19002782-8 1998 This review summarizes the current status of knowledge on the domain organization, topology and higher order structure of P-glycoprotein, the location of drug- and ATP binding sites within P-glycoprotein, its ATPase and drug transport activities, its possible functions as an ion channel, ATP channel and lipid transporter, its potential role in cholesterol biosynthesis, and the effects of phosphorylation on P-glycoprotein activity. Cholesterol 346-357 ATP binding cassette subfamily B member 1 Homo sapiens 122-136 33672718-2 2021 Among these transporters, ABCB11 secretes bile acids, ABCB4 translocates phosphatidylcholine and ABCG5/G8 is responsible for cholesterol secretion, while ABCB1 and ABCC2 transport a variety of drugs and other compounds. Cholesterol 125-136 ATP binding cassette subfamily B member 1 Homo sapiens 26-31 9507994-5 1998 Progesterone, verapamil, and trifluoperazine, inhibitors of p-glycoprotein which are known to inhibit cholesterol transport from the plasma membrane to the endoplasmic reticulum, reduced the amount of newly synthesized cholesterol reaching the plasma membrane. Cholesterol 102-113 ATP binding cassette subfamily B member 1 Homo sapiens 60-74 9507994-5 1998 Progesterone, verapamil, and trifluoperazine, inhibitors of p-glycoprotein which are known to inhibit cholesterol transport from the plasma membrane to the endoplasmic reticulum, reduced the amount of newly synthesized cholesterol reaching the plasma membrane. Cholesterol 219-230 ATP binding cassette subfamily B member 1 Homo sapiens 60-74 7595077-12 1995 However, the results suggest a possible role for p-glycoprotein in normal cholesterol trafficking and triacylglycerol-rich lipoprotein secretion in CaCo-2 cells. Cholesterol 74-85 ATP binding cassette subfamily B member 1 Homo sapiens 49-63 34915026-2 2022 ABCB1 activity is directly dependent on its lipid microenvironment, localizing to cholesterol- and sphingomyelin-rich domains. Cholesterol 82-93 ATP binding cassette subfamily B member 1 Homo sapiens 0-5 34506133-4 2021 Here we compare the conformational ensembles of P-gp embedded in a POPC/cholesterol bilayer generated over 500 ns of replicate simulation with five force fields from popular biomolecular families: AMBER 99SB-ILDN, CHARMM 36, OPLS-AA/L, GROMOS 54A7, and MARTINI. Cholesterol 72-83 ATP binding cassette subfamily B member 1 Homo sapiens 48-52 33350827-0 2021 Cholesterol Asymmetrically Modulates the Conformational Ensemble of the Nucleotide-Binding Domains of P-Glycoprotein in Lipid Nanodiscs. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 102-116 33350827-5 2021 We aimed to determine the effects of cholesterol in a membrane on the conformational behavior of P-gp in lipid nanodiscs. Cholesterol 37-48 ATP binding cassette subfamily B member 1 Homo sapiens 97-101 33350827-7 2021 Hydrogen/deuterium exchange mass spectrometry demonstrates that cholesterol in the membrane induces asymmetric, long-range changes in the distributions and exchange kinetics of conformations of the nucleotide-binding domains, correlating the effects of lipid composition on activity with specific changes in the P-gp conformational landscape. Cholesterol 64-75 ATP binding cassette subfamily B member 1 Homo sapiens 312-316 31676371-0 2020 Probing cholesterol binding and translocation in P-glycoprotein. Cholesterol 8-19 ATP binding cassette subfamily B member 1 Homo sapiens 49-63 32325757-1 2020 Cholesterol derivatives of nuclease-resistant, anti-MDR1 small-interfering RNAs were designed to contain a 2"-OMe-modified 21-bp siRNA and a 63-bp TsiRNA in order to investigate their accumulation and silencing activity in vitro and in vivo. Cholesterol 0-11 ATP binding cassette subfamily B member 1 Homo sapiens 52-56 32343987-5 2020 The enrichment of P-gp and Hsp90 at the cholesterol-rich membrane microdomains is found obligatory for enhanced drug efflux activity. Cholesterol 40-51 ATP binding cassette subfamily B member 1 Homo sapiens 18-22 31676371-4 2020 In this study, a set of 40 independent molecular dynamics (MD) simulations of Pgp embedded in cholesterol-rich lipid bilayers are reported, totaling 8 mus, enabling extensive sampling of cholesterol-protein interactions in Pgp. Cholesterol 187-198 ATP binding cassette subfamily B member 1 Homo sapiens 78-81 31676371-4 2020 In this study, a set of 40 independent molecular dynamics (MD) simulations of Pgp embedded in cholesterol-rich lipid bilayers are reported, totaling 8 mus, enabling extensive sampling of cholesterol-protein interactions in Pgp. Cholesterol 187-198 ATP binding cassette subfamily B member 1 Homo sapiens 223-226 31676371-2 2020 Substrate binding and transport in Pgp are modulated by the presence of cholesterol in the membrane. Cholesterol 72-83 ATP binding cassette subfamily B member 1 Homo sapiens 35-38 31676371-5 2020 Clustering analyses of the ensemble of cholesterol molecules (~5740) sampled around Pgp in these simulations reveal specific and asymmetric cholesterol-binding regions formed by the transmembrane (TM) helices TM1-6 and TM8. Cholesterol 39-50 ATP binding cassette subfamily B member 1 Homo sapiens 84-87 31676371-5 2020 Clustering analyses of the ensemble of cholesterol molecules (~5740) sampled around Pgp in these simulations reveal specific and asymmetric cholesterol-binding regions formed by the transmembrane (TM) helices TM1-6 and TM8. Cholesterol 140-151 ATP binding cassette subfamily B member 1 Homo sapiens 84-87 31676371-7 2020 Binding of cholesterol to Pgp occurs more frequently through its rough beta-face formed by the two protruding methyl groups, whereas the opposite smooth alpha-face prefers packing alongside the membrane lipids. Cholesterol 11-22 ATP binding cassette subfamily B member 1 Homo sapiens 26-29 31676371-4 2020 In this study, a set of 40 independent molecular dynamics (MD) simulations of Pgp embedded in cholesterol-rich lipid bilayers are reported, totaling 8 mus, enabling extensive sampling of cholesterol-protein interactions in Pgp. Cholesterol 94-105 ATP binding cassette subfamily B member 1 Homo sapiens 78-81 31676371-8 2020 One full and two partial cholesterol flipping events between the two leaflets of the bilayer mediated by the surface of Pgp are also captured in these simulations. Cholesterol 25-36 ATP binding cassette subfamily B member 1 Homo sapiens 120-123 31676371-10 2020 Our study is the first to report direct observation of unconventional cholesterol translocation on the surface of Pgp, providing a secondary transport model for the known flippase activity of ABC exporters of cholesterol. Cholesterol 70-81 ATP binding cassette subfamily B member 1 Homo sapiens 114-117 31676371-10 2020 Our study is the first to report direct observation of unconventional cholesterol translocation on the surface of Pgp, providing a secondary transport model for the known flippase activity of ABC exporters of cholesterol. Cholesterol 209-220 ATP binding cassette subfamily B member 1 Homo sapiens 114-117 31718159-5 2019 DOX and P-glycoprotein (P-gp) siRNA were loaded onto the MVs through incubation and cholesterol-mediated methods, achieving high loading rates and targeted tumor delivery. Cholesterol 84-95 ATP binding cassette subfamily B member 1 Homo sapiens 8-22 31718159-5 2019 DOX and P-glycoprotein (P-gp) siRNA were loaded onto the MVs through incubation and cholesterol-mediated methods, achieving high loading rates and targeted tumor delivery. Cholesterol 84-95 ATP binding cassette subfamily B member 1 Homo sapiens 24-28