PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24525336-1 2014 The formation of cholesterol gallstones is a very complex and polygenic disorder that involves an alteration of the secretion of bile lipids, cholesterol crystallization, important immunological reactions in the gallbladder tissue, formation of biliary sludge composed of mucin, and inadequate gallbladder motility. Cholesterol 17-28 LOC100508689 Homo sapiens 272-277 24980223-13 2014 CONCLUSION: Chronic cholesterol depletion in the plasma membrane of NCI-H292 cells may be considered an antihypersecretory method, because it effectively inhibits mucin gene expression of human airway epithelial cells. Cholesterol 20-31 LOC100508689 Homo sapiens 163-168 15834935-9 2005 In conclusion, the results indicate that crystallization-promoting compounds (mucin) are of great importance in the development of cholesterol crystals and gallstones in obese subjects during weight reduction, probably because of defective gallbladder emptying. Cholesterol 131-142 LOC100508689 Homo sapiens 78-83 17696963-7 2007 CONCLUSIONS: Our results support a promoting effect of gallbladder mucin hypersecretion by lipid peroxidation leading to rapid formation of cholesterol crystals in gallbladder bile. Cholesterol 140-151 LOC100508689 Homo sapiens 67-72 16927188-0 2006 Formation of cholesterol crystals at a mucin coated substrate. Cholesterol 13-24 LOC100508689 Homo sapiens 39-44 16927188-2 2006 METHODS: AFM images of phosphatidylcholine/cholesterol vesicles were obtained using a mucin-coated mica substrate. Cholesterol 43-54 LOC100508689 Homo sapiens 86-91 15002984-0 2004 Interaction of mucin with cholesterol enriched vesicles: role of mucin structural domains. Cholesterol 26-37 LOC100508689 Homo sapiens 15-20 15618836-0 2004 Effect of gallbladder mucin on the crystallization of cholesterol in bile. Cholesterol 54-65 LOC100508689 Homo sapiens 22-27 15618836-1 2004 OBJECTIVES: Mucin is supposed to accelerate the crystallization of cholesterol in model bile while studies in native human gallbladder bile revealed conflicting results. Cholesterol 67-78 LOC100508689 Homo sapiens 12-17 15618836-2 2004 METHODS: Therefore, we determined the relation of mucin concentration and cholesterol crystal observation time in gallbladder bile of 73 patients with cholesterol and mixed and 21 patients with pigment stones. Cholesterol 74-85 LOC100508689 Homo sapiens 50-55 15618836-2 2004 METHODS: Therefore, we determined the relation of mucin concentration and cholesterol crystal observation time in gallbladder bile of 73 patients with cholesterol and mixed and 21 patients with pigment stones. Cholesterol 151-162 LOC100508689 Homo sapiens 50-55 15618836-6 2004 However, higher amounts of newly formed cholesterol crystals were seen in bile samples supplemented with human mucin in comparison to negative controls. Cholesterol 40-51 LOC100508689 Homo sapiens 111-116 15002984-1 2004 We utilized fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) to examine the role of gallbladder mucin (GBM) in promoting the aggregation and/or fusion of cholesterol enriched vesicles. Cholesterol 203-214 LOC100508689 Homo sapiens 145-150 12517055-4 2002 In this study, we evaluated mucin gene expression in gallbladders containing cholesterol stones or calcium bilirubinate stones, and gallbladders without stones. Cholesterol 77-88 LOC100508689 Homo sapiens 28-33 12395324-10 2002 Gallstone and mucin loci colocalized with potential QTLs affecting the formation of cholesterol crystals. Cholesterol 84-95 LOC100508689 Homo sapiens 14-19 12517055-10 2002 CONCLUSION: Altered mucin gene expression was found in gallbladders with cholesterol stones and calcium bilirubinate stones, as evidenced by the presence of MUC2 and MUC4 and the increased expression of MUC1, MUC3, MUC5B and MUC6. Cholesterol 73-84 LOC100508689 Homo sapiens 20-25 11475792-8 2001 The mucin gel is thought to favour gallstone formation by promoting cholesterol crystal precipitation and aggregation. Cholesterol 68-79 LOC100508689 Homo sapiens 4-9 12032489-1 2002 A series of our studies have shown that formation of cholesterol-supersaturated bile in patients with cholesterol gallstone disease is causatively related to decreased gallbladder contractility and mucin hypersecretion by the gallbladder. Cholesterol 53-64 LOC100508689 Homo sapiens 198-203 11019989-6 2000 The sediment appeared as a mixture of vesicular aggregates and pigment particles which were linked by a gel matrix of mucin containing cholesterol crystals. Cholesterol 135-146 LOC100508689 Homo sapiens 118-123 11207603-2 2000 Cholesterol inhibits the LLO-induced mucin exocytosis, whereas the oxidized form of cholesterol had no inhibitory effect. Cholesterol 0-11 LOC100508689 Homo sapiens 37-42 11207603-3 2000 LLO-induced mucin exocytosis inhibited by cholesterol can be restored by enzymatic treatment with cholesterol oxidase. Cholesterol 42-53 LOC100508689 Homo sapiens 12-17 11207603-4 2000 Inhibition of cholesterol synthesis in HT29-MTX cells results in a decrease in the LLO-induced mucin exocytosis. Cholesterol 14-25 LOC100508689 Homo sapiens 95-100 10961723-2 2000 Lysolecithin and polyunsaturated free fatty acids are known to stimulate the synthesis and/or secretion of gallbladder mucin via a prostanoid pathway, leading to enhancing cholesterol crystal nucleation and growth, and therefore, the action of phospholipase A2 is associated, in part, with bile phospholipid fatty acid. Cholesterol 172-183 LOC100508689 Homo sapiens 119-124 10064729-12 1999 For pathophysiologically relevant conditions, our results clarify that mucin gel and cholesterol content, but not soluble biliary glycoproteins, promote cholesterol crystallization in human gallbladder bile. Cholesterol 153-164 LOC100508689 Homo sapiens 71-76 9741686-5 1998 Mucin also stimulated, and with soluble calcium or calcium phosphate precipitates additively increased, the cholesterol crystal mass. Cholesterol 108-119 LOC100508689 Homo sapiens 0-5 7657293-0 1995 Mucin-vesicle interactions in model bile: evidence for vesicle aggregation and fusion before cholesterol crystal formation. Cholesterol 93-104 LOC100508689 Homo sapiens 0-5 9741686-1 1998 Human biliary mucin and calcium binding protein (CBP) influence formation of both calcium salt precipitates and cholesterol crystals and colocalize in the center of cholesterol gallstones. Cholesterol 112-123 LOC100508689 Homo sapiens 14-19 8895014-1 1996 BACKGROUND/AIMS: Cholesterol gallstones consist of cholesterol crystals and smaller amounts of pigments and calcium salts, arrayed on a mucin plus protein matrix. Cholesterol 17-28 LOC100508689 Homo sapiens 136-141 8895014-7 1996 Mucin was identified in a three-dimensional network intercalated between cholesterol crystals and as septa between deposits of pigments and cholesterol; APF/CBP and ApN coated only the pigment deposits. Cholesterol 73-84 LOC100508689 Homo sapiens 0-5 8895014-7 1996 Mucin was identified in a three-dimensional network intercalated between cholesterol crystals and as septa between deposits of pigments and cholesterol; APF/CBP and ApN coated only the pigment deposits. Cholesterol 140-151 LOC100508689 Homo sapiens 0-5 9007718-8 1996 This may lead to a higher concentration of mucin in gallbladder bile and thus an increased risk of precipitation of cholesterol crystals and gallstone formation. Cholesterol 116-127 LOC100508689 Homo sapiens 43-48 7826359-1 1995 In this study, the interaction of mucin and concanavalin A-binding proteins isolated from human bile with cholesterol/phospholipid vesicles was investigated. Cholesterol 106-117 LOC100508689 Homo sapiens 34-39 8482190-0 1993 Isolation of mucin from human hepatic bile and its induced effects on precipitation of cholesterol and calcium carbonate in vitro. Cholesterol 87-98 LOC100508689 Homo sapiens 13-18 8482190-4 1993 The mucin at as low a concentration as 100 micrograms/ml significantly shortened the cholesterol nucleation time in the supersaturated model bile, mimicking human hepatic bile. Cholesterol 85-96 LOC100508689 Homo sapiens 4-9 8482190-6 1993 Taking account of that both cholesterol and calcium salts are major constituents of ductular gallstones, we conclude that biliary mucin is likely to play an important regulating role in the formation of ductular stones. Cholesterol 28-39 LOC100508689 Homo sapiens 130-135 35227133-0 2022 Cholesterol crystals activate NLRP3 inflammasomes and promote gallstone formation by increasing mucin secretion. Cholesterol 0-11 LOC100508689 Homo sapiens 96-101 8441906-7 1993 These findings suggest that in humans, hepatic cholesterol hypersecretion is associated with the increased unsaturated fatty acid proportion in biliary phospholipids and gallbladder mucin hypersecretion, thereby causing rapid cholesterol crystal nucleation. Cholesterol 47-58 LOC100508689 Homo sapiens 182-187 8441906-7 1993 These findings suggest that in humans, hepatic cholesterol hypersecretion is associated with the increased unsaturated fatty acid proportion in biliary phospholipids and gallbladder mucin hypersecretion, thereby causing rapid cholesterol crystal nucleation. Cholesterol 226-237 LOC100508689 Homo sapiens 182-187 1529152-6 1992 Crystallisation results in suspension of cholesterol crystals or bilirubinate salts in gallbladder mucin gel and is known as "biliary sludge". Cholesterol 41-52 LOC100508689 Homo sapiens 99-104 2210258-2 1990 Gallbladder mucin is a densely glycosylated macro-molecule that promotes cholesterol gallstone formation in experimental animals and in humans. Cholesterol 73-84 LOC100508689 Homo sapiens 12-17 2210647-5 1990 Mucin contains domains that bind cholesterol and lecithin transported as vesicles in supersaturated bile. Cholesterol 33-44 LOC100508689 Homo sapiens 0-5 2210647-6 1990 Furthermore, mucin accelerates the nucleation of cholesterol crystals in both supersaturated model and native biles. Cholesterol 49-60 LOC100508689 Homo sapiens 13-18 2210647-7 1990 Binding of cholesterol-enriched vesicles to hydrophobic domains on the mucin protein core appears to be critical for the acceleration of cholesterol crystal nucleation by mucin. Cholesterol 11-22 LOC100508689 Homo sapiens 71-76 2210647-7 1990 Binding of cholesterol-enriched vesicles to hydrophobic domains on the mucin protein core appears to be critical for the acceleration of cholesterol crystal nucleation by mucin. Cholesterol 11-22 LOC100508689 Homo sapiens 171-176 2210647-7 1990 Binding of cholesterol-enriched vesicles to hydrophobic domains on the mucin protein core appears to be critical for the acceleration of cholesterol crystal nucleation by mucin. Cholesterol 137-148 LOC100508689 Homo sapiens 71-76 2210647-7 1990 Binding of cholesterol-enriched vesicles to hydrophobic domains on the mucin protein core appears to be critical for the acceleration of cholesterol crystal nucleation by mucin. Cholesterol 137-148 LOC100508689 Homo sapiens 171-176 35227133-9 2022 We found that inflammasome activation participates in cholesterol crystal induced mucin secretion and gallstone development. Cholesterol 54-65 LOC100508689 Homo sapiens 82-87 2595266-1 1989 We measured gallbladder mucin production by hamsters fed diets lithogenic for either cholesterol or pigment gallstones. Cholesterol 85-96 LOC100508689 Homo sapiens 24-29 2595266-2 1989 In hamsters on the cholesterol stone diet, gallbladder production of 3H-glucosamine-labeled mucin was elevated two- and seven-fold after 1 and 3 weeks, respectively. Cholesterol 19-30 LOC100508689 Homo sapiens 92-97 3655561-0 1987 Human gallbladder mucin binds biliary lipids and promotes cholesterol crystal nucleation in model bile. Cholesterol 58-69 LOC100508689 Homo sapiens 18-23 2666254-14 1989 In experimental cholesterol gallstone formation, prostaglandins are involved in the production of mucin, which acts as a nidus for stone formation, and cyclooxygenase inhibitors prevent the formation of experimental cholesterol gallstones. Cholesterol 16-27 LOC100508689 Homo sapiens 98-103 3209106-7 1988 Neither do the data indicate that the formation and occurrence of cholesterol crystals in gall bladder bile from gall stone patients is caused by an increased concentration of mucin. Cholesterol 66-77 LOC100508689 Homo sapiens 176-181 3655561-1 1987 The binding of phosphatidylcholine and cholesterol in model bile to human gallbladder mucin was studied by means of a rapid filtration binding assay and sucrose density gradient ultracentrifugation. Cholesterol 39-50 LOC100508689 Homo sapiens 86-91 3655561-2 1987 Numerous low affinity binding sites for phosphatidylcholine and cholesterol were present on gallbladder mucin. Cholesterol 64-75 LOC100508689 Homo sapiens 104-109 3655561-3 1987 Binding of phosphatidylcholine and cholesterol to mucin increased as a function of cholesterol saturation index. Cholesterol 35-46 LOC100508689 Homo sapiens 50-55 3655561-3 1987 Binding of phosphatidylcholine and cholesterol to mucin increased as a function of cholesterol saturation index. Cholesterol 83-94 LOC100508689 Homo sapiens 50-55 3655561-5 1987 Proteolytic digestion also resulted in a 91% and 78% decrease, respectively, in the binding of phosphatidylcholine and cholesterol to mucin. Cholesterol 119-130 LOC100508689 Homo sapiens 134-139 3655561-9 1987 These data indicate that highly purified human gallbladder mucin binds phosphatidylcholine and cholesterol in model bile. Cholesterol 95-106 LOC100508689 Homo sapiens 59-64 6428962-0 1984 Human gallbladder mucin accelerates nucleation of cholesterol in artificial bile. Cholesterol 50-61 LOC100508689 Homo sapiens 18-23 3967040-4 1985 Conditions known to enhance cholesterol absorption in vivo also decreased mucin complexing to cholesterol in vitro. Cholesterol 28-39 LOC100508689 Homo sapiens 74-79 3967040-4 1985 Conditions known to enhance cholesterol absorption in vivo also decreased mucin complexing to cholesterol in vitro. Cholesterol 94-105 LOC100508689 Homo sapiens 74-79 3967040-5 1985 This association of cholesterol and intestinal surface mucin also occurred during incubations of intestinal segments with dispersed cholesterol, resulting in a high level of intestinal adsorption, with little or no cellular absorption of the sterol. Cholesterol 20-31 LOC100508689 Homo sapiens 55-60 3967040-5 1985 This association of cholesterol and intestinal surface mucin also occurred during incubations of intestinal segments with dispersed cholesterol, resulting in a high level of intestinal adsorption, with little or no cellular absorption of the sterol. Cholesterol 132-143 LOC100508689 Homo sapiens 55-60 3967040-7 1985 The results suggest that surface mucin binding of cholesterol may represent at least one major diffusion limitation to cholesterol absorption in the intestine. Cholesterol 50-61 LOC100508689 Homo sapiens 33-38 3967040-7 1985 The results suggest that surface mucin binding of cholesterol may represent at least one major diffusion limitation to cholesterol absorption in the intestine. Cholesterol 119-130 LOC100508689 Homo sapiens 33-38 6546237-10 1984 These findings suggest that inhibition of mucin release may prevent cholesterol stone formation during high-risk periods or after dissolution therapy with bile salts. Cholesterol 68-79 LOC100508689 Homo sapiens 42-47 6428962-3 1984 Human gallbladder mucin at concentrations of 2 and 4 mg/ml accelerated the nucleation time of cholesterol crystals in model bile. Cholesterol 94-105 LOC100508689 Homo sapiens 18-23 6428962-4 1984 The mean number of cholesterol crystals in artificial bile incubated for 10 days with 4 mg/ml of human gallbladder mucin was 2327/mm3 (p less than 0.01) vs. control of 51/mm3. Cholesterol 19-30 LOC100508689 Homo sapiens 115-120 6428962-7 1984 Nucleation by human gallbladder mucin was significantly increased only with cholesterol saturation indices greater than 1.0, and in biles containing 10% but not 3% total lipid by weight. Cholesterol 76-87 LOC100508689 Homo sapiens 32-37 6428962-10 1984 These observations provide further evidence that human gallbladder mucin may contribute to cholesterol gallstone formation in humans by accelerating nucleation of cholesterol monohydrate crystals from supersaturated gallbladder bile. Cholesterol 91-102 LOC100508689 Homo sapiens 67-72 7240416-12 1981 Purified human gallbladder mucin gels were shown to induce nucleation of lecithin-cholesterol liquid crystals from supersaturated hepatic bile. Cholesterol 82-93 LOC100508689 Homo sapiens 27-32 6873615-6 1983 These results indicate that mucin can bind bilirubin in vitro, and might explain their occurrence in the nucleus of human cholesterol stones. Cholesterol 122-133 LOC100508689 Homo sapiens 28-33 13398510-2 1957 The possibility of cholesterol in mucin. Cholesterol 19-30 LOC100508689 Homo sapiens 34-39