PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 25878100-2 2015 One such antibody, PGT135, contacts the intrinsic mannose patch of gp120 at the Asn332, Asn392, and Asn386 glycosylation sites. Mannose 50-57 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 67-72 27669574-2 2016 Each binds to high-mannose glycans on the surface envelope glycoprotein gp120, yet the mechanisms by which they engage viral spikes and exhibit inhibition constants ranging from nanomolar to picomolar are not understood. Mannose 19-26 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 72-77 26837192-1 2016 BACKGROUND: Broadly neutralizing antibodies (bnAbs) directed against the mannose-patch on the HIV envelope glycoprotein gp120 have several features that make them desirable targets for vaccine design. Mannose 73-80 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 120-125 26611567-4 2016 DC-SIGN binds mannose or fucose-containing carbohydrates from viral proteins such as the HIV envelope glycoprotein gp120. Mannose 14-21 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 115-120 26540494-2 2015 Compounds that interact with these high-mannose type glycans may disturb the interaction between gp120 and its (co)receptors and are considered potential anti-HIV agents. Mannose 40-47 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 97-102 24965454-2 2014 2G12 is a unique, domain-exchanged antibody that binds exclusively to conserved N-linked glycans that form the high-mannose patch on the gp120 outer domain centered on a glycan at position N332. Mannose 116-123 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 137-142 25132301-6 2014 METHODS: N-Glycans were released from ribonuclease B, ovalbumin and gp120 with endoH to give high-mannose and hybrid glycans, and from IgG with endoS to produce biantennary complex glycans, all missing the reducing-terminal GlcNAc residue. Mannose 98-105 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 68-73 24074568-3 2013 Sequence analysis of gp120 showed that most had deletions of 1 to 5 mannose-rich glycans. Mannose 68-75 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 21-26 24884609-9 2014 Site N274 contained high-mannose N-linked glycans in both serum and recombinant ITIH4. Mannose 25-32 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 80-85 24828077-0 2014 Promiscuous glycan site recognition by antibodies to the high-mannose patch of gp120 broadens neutralization of HIV. Mannose 62-69 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 79-84 24825317-3 2014 Our results show that mannose binding lectin (MBL) that binds to the N-linked mannose residues on gp120, participates in intravesicular packaging of gp120 in neuronal subcellular organelles and also in subcellular trafficking of these vesicles in neuronal cells. Mannose 22-29 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 98-103 24825317-3 2014 Our results show that mannose binding lectin (MBL) that binds to the N-linked mannose residues on gp120, participates in intravesicular packaging of gp120 in neuronal subcellular organelles and also in subcellular trafficking of these vesicles in neuronal cells. Mannose 22-29 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 149-154 23741304-8 2013 SPR-analysis also demonstrated that wild-type GRFT and its single mutant CBS variants have the capacity to expel bound gp120 from the gp120-DC-SIGN complex in a dose dependent manner, a property that was not observed for HHA, another mannose-specific potent anti-HIV-1 CBA. Mannose 234-241 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 119-124 23339644-7 2013 For N-linked glycosylation, two sites (N386 and N392) in the V4 region were populated with high mannose glycans in the CHO cell-derived 1086.C gp120, while these sites had a mixture of high mannose and processed glycans in the 293T cell-derived 1086.C gp120. Mannose 96-103 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 143-148 23339644-7 2013 For N-linked glycosylation, two sites (N386 and N392) in the V4 region were populated with high mannose glycans in the CHO cell-derived 1086.C gp120, while these sites had a mixture of high mannose and processed glycans in the 293T cell-derived 1086.C gp120. Mannose 96-103 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 252-257 21268157-9 2011 This information is of key importance for the development of multivalent synthetic gp120 high-mannose glycoconjugate mimics in the context of vaccine development. Mannose 94-101 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 83-88 23100517-7 2012 Receptor-blocking and cross-linking studies showed that these inhibitory effects of gp120 were mediated by interactions with CD4 and mannose-binding C-type lectin receptors, but not with the chemokine receptors CCR5 and CXCR4. Mannose 133-140 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 84-89 22628288-3 2012 Among these, the monoclonal antibody 2G12 binds to clusters of high-mannose-type glycans that are present on the surface of gp120. Mannose 68-75 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 124-129 23527085-5 2013 The binding of SP-D to HIV particles and gp120 was inhibited by the presence of several hexoses with mannose found to be the strongest inhibitor. Mannose 101-108 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 41-46 21793733-6 2012 We conclude that gp120-GRFT complexes are more immunogenic than the free proteins, for both components, and that occluding the mannose moieties on monomeric gp120 can improve the humoral immune response to this protein. Mannose 127-134 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 17-22 21793733-6 2012 We conclude that gp120-GRFT complexes are more immunogenic than the free proteins, for both components, and that occluding the mannose moieties on monomeric gp120 can improve the humoral immune response to this protein. Mannose 127-134 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 157-162 21674342-0 2011 On-resin convergent synthesis of a glycopeptide from HIV gp120 containing a high mannose type N-linked oligosaccharide. Mannose 81-88 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 57-62 20932025-2 2010 In this report we aimed to investigate the potential of mannose-containing glycopolymers to interact with human DC-SIGN and the ability of these glycopolymers to inhibit the interactions between DC-SIGN and the HIV envelope glycoprotein gp120. Mannose 56-63 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 237-242 20080975-7 2010 An enzyme-linked immunosorbent assay confirmed direct binding of BanLec to gp120 and indicated that BanLec can recognize the high mannose structures that are recognized by the monoclonal antibody 2G12. Mannose 130-137 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 75-80 20825246-5 2010 We mapped the glycosylation sites and calculated the glycosylation occupancy of gp120-OD8; 11 sites from 15 glycosylation motifs were determined as having high-mannose or hybrid glycosylation structures. Mannose 160-167 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 80-85 19920089-2 2010 Compared to normal mammalian glycoproteins, high-mannose-type glycans are disproportionately represented on the gp120 subunit of Env. Mannose 49-56 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 112-117 19920089-6 2010 The gp120 binding activity of these immune sera was due to mannose-specific immunoglobulin, as removal of high-mannose glycans and alpha1,2-linked mannoses from gp120 abrogated serum binding. Mannose 111-118 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 4-9 19920089-6 2010 The gp120 binding activity of these immune sera was due to mannose-specific immunoglobulin, as removal of high-mannose glycans and alpha1,2-linked mannoses from gp120 abrogated serum binding. Mannose 111-118 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 161-166 19920089-6 2010 The gp120 binding activity of these immune sera was due to mannose-specific immunoglobulin, as removal of high-mannose glycans and alpha1,2-linked mannoses from gp120 abrogated serum binding. Mannose 59-66 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 4-9 19920089-6 2010 The gp120 binding activity of these immune sera was due to mannose-specific immunoglobulin, as removal of high-mannose glycans and alpha1,2-linked mannoses from gp120 abrogated serum binding. Mannose 59-66 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 161-166 16616922-0 2006 Mannose hyperbranched dendritic polymers interact with clustered organization of DC-SIGN and inhibit gp120 binding. Mannose 0-7 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 101-106 19410272-3 2009 For example, gp120 mannose residues can induce immunosuppressive responses in vitro, including IL-10 expression, via mannose C-type lectin receptors on antigen-presenting cells. Mannose 19-26 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 13-18 19147415-3 2009 It acts by direct binding to mannose residues that are abundantly present on the HIV gp120 envelope and so interrupts the virus entry process. Mannose 29-36 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 85-90 18566427-5 2008 Digestion of gp120 with endoglycosidase H abrogates the binding of SP-A, indicating that the high mannose structures on gp120 are the target of the collectin. Mannose 98-105 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 13-18 17658575-3 2007 Genotypic and phenotypic characterization of resistant env clones indicated that 3-5 high-mannose residues from 289 to 448 in the C2-C4 region of gp120 were mutated and correlated with the resistance levels. Mannose 90-97 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 146-151 17983270-5 2007 The mannose-binding protein cyanovirin-N, the 2G12 mAb to a mannose-dependent gp120 epitope, and MCLR-specific mAbs inhibited IL-10 expression, as did enzymatic removal of gp120 mannose moieties, whereas inhibitors of signaling via CD4, CCR5, or CXCR4 were ineffective. Mannose 60-67 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 78-83 19681073-1 2009 The HIV envelope glycoprotein gp120 takes advantage of the high-mannose clusters on its surface to target the C-type lectin dendritic cell-specific intracellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) on dendritic cells. Mannose 64-71 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 30-35 18566427-5 2008 Digestion of gp120 with endoglycosidase H abrogates the binding of SP-A, indicating that the high mannose structures on gp120 are the target of the collectin. Mannose 98-105 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 120-125 18434410-0 2008 An engineered Saccharomyces cerevisiae strain binds the broadly neutralizing human immunodeficiency virus type 1 antibody 2G12 and elicits mannose-specific gp120-binding antibodies. Mannose 139-146 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 156-161 18436959-2 2008 The wild-type (wt) protein binds with high affinity to mannose-rich oligosaccharides on the surface of gp120 through two quasi-symmetric sites, located in domains A and B. Mannose 55-62 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 103-108 17983270-0 2007 HIV-1 gp120 mannoses induce immunosuppressive responses from dendritic cells. Mannose 12-20 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 6-11 17983270-5 2007 The mannose-binding protein cyanovirin-N, the 2G12 mAb to a mannose-dependent gp120 epitope, and MCLR-specific mAbs inhibited IL-10 expression, as did enzymatic removal of gp120 mannose moieties, whereas inhibitors of signaling via CD4, CCR5, or CXCR4 were ineffective. Mannose 4-11 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 78-83 17983270-5 2007 The mannose-binding protein cyanovirin-N, the 2G12 mAb to a mannose-dependent gp120 epitope, and MCLR-specific mAbs inhibited IL-10 expression, as did enzymatic removal of gp120 mannose moieties, whereas inhibitors of signaling via CD4, CCR5, or CXCR4 were ineffective. Mannose 4-11 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 172-177 17983270-5 2007 The mannose-binding protein cyanovirin-N, the 2G12 mAb to a mannose-dependent gp120 epitope, and MCLR-specific mAbs inhibited IL-10 expression, as did enzymatic removal of gp120 mannose moieties, whereas inhibitors of signaling via CD4, CCR5, or CXCR4 were ineffective. Mannose 60-67 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 78-83 17123566-5 2007 Moreover, we observed that the NL4.3-2G12-resistant virus, with the N295K mutation in gp120, became significantly more sensitive to several mannose-specific lectins. Mannose 140-147 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 86-91 15613479-9 2005 Further data showed that the binding of GRFT to soluble gp120 was inhibited by the monosaccharides glucose, mannose, and N-acetylglucosamine but not by galactose, xylose, fucose, N-acetylgalactosamine, or sialic acid-containing glycoproteins. Mannose 108-115 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 56-61 15917430-6 2005 Molecular structure comparison for Man9GlcNAc2 recognition by ConA and 2G12 indicates that 2G12 has a more restricted specificity to high mannose glycans of gp120 which correlates with kinetic analysis assessed by surface plasmon resonance (SPR) and ConA inhibits 2G12 binding to gp120 but 2G12 does not inhibit ConA binding to gp120. Mannose 138-145 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 157-162 7474402-4 1995 The inhibitory effect was suppressed by addition of high mannose type oligosaccharides of gp120. Mannose 57-64 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 90-95 12626400-5 2003 Previous studies have shown that DC-SIGN binds HIV gp120 that contains high-mannose-type N-glycans. Mannose 76-83 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 51-56 12565922-2 2003 Thus, the high-mannose type glycopeptides [gp120 (336-342)] containing Man(9), Man(6) and Man(5) moieties, respectively, were synthesized in satisfactory yields via transglycosylation to the acetylglucosaminyl peptide, using the recombinant Arthrobacter Endo-beta-N-acetyl-glucosaminidase (Endo-A) as the key enzyme. Mannose 15-22 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 43-48 16851402-2 2005 Recently, it has been experimentally demonstrated that cyanovirin binds mannose oligomers on the surface of glycoprotein gp120. Mannose 72-79 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 121-126 15450746-5 2004 Secretory IgA glycans bind gut bacteria, and an unusual cluster of mannose residues on gp120, the surface coat protein of the HIV virus, is recognized by the novel "domain-swapped" IgG 2G12 serum antibody. Mannose 67-74 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 87-92 14970226-6 2004 Monosaccharides with equatorial 4-OH groups competed as well as D-mannose for gp120 binding to DC-SIGN, regardless of how the other hydroxyl groups were positioned. Mannose 64-73 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 78-83 15078900-8 2004 Additional studies using immature monocyte-derived dendritic cells demonstrated that although mannose-binding C-type lectin receptors and CD4 are the principal receptors for gp120, other mechanisms may account for virus capture. Mannose 94-101 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 174-179 12072529-0 2002 The broadly neutralizing anti-human immunodeficiency virus type 1 antibody 2G12 recognizes a cluster of alpha1-->2 mannose residues on the outer face of gp120. Mannose 118-125 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 156-161 12072529-8 2002 Consistent with this finding, the binding of 2G12 to gp120 could be inhibited by monomeric mannose but not by galactose, glucose, or N-acetylglucosamine. Mannose 91-98 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 53-58 11714623-5 2001 Other cell surface receptors including mannose binding protein on macrophages and DC-SIGN on dendritic cells also interact with gp120 on virus particles but do not actively promote fusion and virus entry. Mannose 39-46 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 128-133 11810515-3 1999 It has been found that the gp120 oligosaccharides are essential in HIV infection and that high-mannose type oligosaccharides present in the gp120 molecule are especially critical. Mannose 95-102 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 140-145 1493050-7 1992 Digestion of immunoprecipitated gp120 and gp160 with endoglycosidase H and peptide N-glycosidase F revealed that the envelope glycoprotein in transfected L cells possessed both high mannose and complex N-glycans, analogous to the posttranslational modification of the mature envelope glycoprotein in infected T cells. Mannose 182-189 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 32-37 8100569-6 1993 In contrast, several lectins (including mannose binding protein) bound to gp120 and blocked CD4-gp120 interactions. Mannose 40-47 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 74-79 8100569-6 1993 In contrast, several lectins (including mannose binding protein) bound to gp120 and blocked CD4-gp120 interactions. Mannose 40-47 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 96-101 8503188-8 1993 These results suggest that although interacting with different regions of gp120, the mannose-specific lectin alters the conformation of the glycoprotein in a manner similar to that induced by sCD4, causing destabilization of the gp120/gp41 complex. Mannose 85-92 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 74-79 8503188-8 1993 These results suggest that although interacting with different regions of gp120, the mannose-specific lectin alters the conformation of the glycoprotein in a manner similar to that induced by sCD4, causing destabilization of the gp120/gp41 complex. Mannose 85-92 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 229-234 1518869-8 1992 gp120 binding is high affinity (kd, 1.3-1.6 nM) and inhibited by mannan, D-mannose, and L-fucose; once bound, gp120 is internalized rapidly. Mannose 73-82 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 0-5 1518869-9 1992 Collectively, these data demonstrate that the gp120-binding protein is a membrane-associated mannose-binding lectin. Mannose 93-100 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 46-51 1518869-8 1992 gp120 binding is high affinity (kd, 1.3-1.6 nM) and inhibited by mannan, D-mannose, and L-fucose; once bound, gp120 is internalized rapidly. Mannose 73-82 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 110-115 2187500-4 1990 Digestion of the purified gp120 and gp160 with endoglycosidases revealed that both proteins are heavily glycosylated and contain complex carbohydrates, in contrast to the intracellular form of gp160 which has been shown to contain mannose-rich immature sugars. Mannose 231-238 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 26-31 2538547-7 1989 Therefore, probably half of the glycan moieties of gp120/160 are composed of high mannose and biantennary chains, the other half being triantennary species. Mannose 82-89 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 51-60 35178612-4 2022 We analyzed the thermodynamic properties of SD1 compared to full-size SVN (produced in E. coli) by isothermal titration and differential scanning calorimetry to characterize the specific interactions between SVN/SD1 and gp120 as well as to high-mannose oligosaccharides. Mannose 245-252 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 220-225 2574581-3 1989 The carbohydrate of gp120 recognized by lectins was thus arranged in at least four types of glycans: a high mannose type glycan, a bisected hybrid or complex type glycan, a biantennary fucosylated complex type glycan and a triantennary bisected complex type glycan. Mannose 108-115 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 20-25 2561054-3 1989 Evidence is presented that the high-mannose-type oligosaccharides with seven, eight and nine mannose residues from both forms of gp120 are recognized by the serum lectin, and that these reactivities are unrelated to CD4 recognition. Mannose 36-43 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 129-134 33388127-3 2020 Herein, we provide a brief overview of the progress made on the development of synthetic carbohydrate-based epitope mimics for the elicitation of bnAbs directed to certain regions on Env gp120 protein: the outer domain high-mannose cluster and the variable loops V1V2 and V3. Mannose 224-231 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 187-192 32729597-1 2020 High-mannose (Man9GlcNAc2) is the main carbohydrate unit present in viral envelope glycoproteins such as gp120 of HIV and the GP1 of Ebola virus. Mannose 5-12 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 105-110 29306566-0 2018 Bispecific chimeric antigen receptors targeting the CD4 binding site and high-mannose Glycans of gp120 optimized for anti-human immunodeficiency virus potency and breadth with minimal immunogenicity. Mannose 78-85 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 97-102 30834312-1 2019 Up to ~20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (~40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP). Mannose 165-172 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 190-195 30834312-1 2019 Up to ~20% of HIV-infected individuals eventually develop broadly neutralizing antibodies (bnAbs), and many of these antibodies (~40%) target a region of dense high-mannose glycosylation on gp120 of the HIV envelope protein, known as the "high-mannose patch" (HMP). Mannose 244-251 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 190-195