PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11161734-3 2001 It was previously reported that IRBP can associate with 2 mol of all-trans-retinol or 2 mol of 11-cis-retinal per mol of protein. Vitamin A 75-82 retinol binding protein 3 Homo sapiens 32-36 11934446-7 2002 IRBP and CRX transcript levels are sensitive to butyrate and CRX expression is sensitive to retinol. Vitamin A 92-99 retinol binding protein 3 Homo sapiens 0-4 15452202-5 2004 Clearance of retinol fluorescence is considerably accelerated by the presence of the exogenous lipophilic substances IRBP (interphotoreceptor retinoid binding protein) and serum albumin. Vitamin A 13-20 retinol binding protein 3 Homo sapiens 117-121 15452202-5 2004 Clearance of retinol fluorescence is considerably accelerated by the presence of the exogenous lipophilic substances IRBP (interphotoreceptor retinoid binding protein) and serum albumin. Vitamin A 13-20 retinol binding protein 3 Homo sapiens 123-166 10756180-1 2000 PURPOSE: The purpose of this study was to measure the effects of mutations on the retinol binding capability of human Repeat 1 of interphotoreceptor retinoid-binding protein (IRBP). Vitamin A 82-89 retinol binding protein 3 Homo sapiens 175-179 11262622-7 2000 CONCLUSION: In the eye, IRBP can stabilise vitamin A and debatably may be responsible for transport of different forms of vitamin A between the photoreceptor cells and pigment epithelium. Vitamin A 43-52 retinol binding protein 3 Homo sapiens 24-28 11262622-7 2000 CONCLUSION: In the eye, IRBP can stabilise vitamin A and debatably may be responsible for transport of different forms of vitamin A between the photoreceptor cells and pigment epithelium. Vitamin A 122-131 retinol binding protein 3 Homo sapiens 24-28 10756180-4 2000 We analyzed the effects of point substitutions on the retinol and fatty acid binding properties of Repeat 1 of human IRBP at 25 and 50 degrees C. METHODS: To find residues critical to retinol binding that might affect function, a series of thirteen mutations were created by site-specific mutagenesis between positions 140 and 280 in Repeat 1 of human IRBP. Vitamin A 184-191 retinol binding protein 3 Homo sapiens 117-121 10756180-10 2000 CONCLUSIONS: Several IRBP mutants containing point mutations retained native structure but lost retinol binding function. Vitamin A 96-103 retinol binding protein 3 Homo sapiens 21-25 10756180-13 2000 This excludes three previously proposed IRBP-retinol binding mechanisms: (1) retinol binds to a small portion of the protein repeat, (2) retinol can bind to any hydrophobic patch in the protein, and (3) native conformation is not required for retinol binding to the repeat. Vitamin A 45-52 retinol binding protein 3 Homo sapiens 40-44 10756181-21 2000 Thus, it is possible that position 239, found in Domain B2 of IRBP Repeat 1, is located in or near one of two retinol binding sites. Vitamin A 110-117 retinol binding protein 3 Homo sapiens 62-66 9374503-0 1997 Binding of retinol in both retinoid-binding sites of interphotoreceptor retinoid-binding protein (IRBP) is stabilized mainly by hydrophobic interactions. Vitamin A 11-18 retinol binding protein 3 Homo sapiens 53-96 9374503-0 1997 Binding of retinol in both retinoid-binding sites of interphotoreceptor retinoid-binding protein (IRBP) is stabilized mainly by hydrophobic interactions. Vitamin A 11-18 retinol binding protein 3 Homo sapiens 98-102 9374503-7 1997 Here, the thermodynamic parameters governing the interactions of retinol with the IRBP retinoid-binding sites were measured. Vitamin A 65-72 retinol binding protein 3 Homo sapiens 82-86 7490779-4 1995 In amphibians and mammals, IRBP consists of four homologous repeats of about 300 amino acids which form two retinol and four fatty acid-binding sites. Vitamin A 108-115 retinol binding protein 3 Homo sapiens 27-31 8218196-0 1993 Interactions of all-trans-retinol and long-chain fatty acids with interphotoreceptor retinoid-binding protein. Vitamin A 16-33 retinol binding protein 3 Homo sapiens 66-109 8218196-6 1993 Long-chain fatty acids were found in this study to displace all-trans-retinol from the stronger retinol-binding site on IRBP. Vitamin A 60-77 retinol binding protein 3 Homo sapiens 120-124 8218196-6 1993 Long-chain fatty acids were found in this study to displace all-trans-retinol from the stronger retinol-binding site on IRBP. Vitamin A 70-77 retinol binding protein 3 Homo sapiens 120-124 8075067-4 1994 It was found that IRBP contains two binding sites for 11-cis-retinaldehyde and for all-trans-retinaldehyde and retinol. Vitamin A 111-118 retinol binding protein 3 Homo sapiens 18-22 8075067-7 1994 11-cis-Retinaldehyde and all-trans-retinol were found to dissociate from the strong binding site of IRBP 3-4-fold slower than all-trans-retinaldehyde and 11-cis-retinol. Vitamin A 25-42 retinol binding protein 3 Homo sapiens 100-104 8075067-7 1994 11-cis-Retinaldehyde and all-trans-retinol were found to dissociate from the strong binding site of IRBP 3-4-fold slower than all-trans-retinaldehyde and 11-cis-retinol. Vitamin A 154-168 retinol binding protein 3 Homo sapiens 100-104 8075067-8 1994 The higher binding affinities and the slower rates of dissociation from IRBP displayed by 11-cis-retinaldehyde and by all-trans-retinol correspond to the physiological need to shuttle these particular retinoids between pigment epithelium and photoreceptor cells across the interphotoreceptor matrix as part of the visual cycle. Vitamin A 127-135 retinol binding protein 3 Homo sapiens 72-76 7835405-0 1994 Exchange of retinol between IRBP and CRBP. Vitamin A 12-19 retinol binding protein 3 Homo sapiens 28-32 7835405-2 1994 Interphotoreceptor retinoid-binding protein (IRBP) is thought to facilitate this exchange, but the transfer of retinol between IRBP and CRBP has not been explored. Vitamin A 111-118 retinol binding protein 3 Homo sapiens 127-131 7835405-3 1994 In this study we used a mixture of purified IRBP and CRBP as a model system to measure the amount and rate of retinol transfer between the two proteins. Vitamin A 110-117 retinol binding protein 3 Homo sapiens 44-48 7835405-4 1994 When retinol is transferred from IRBP to CRBP, its absorbance maximum shifts from 330 to 350 nm. Vitamin A 5-12 retinol binding protein 3 Homo sapiens 33-37 7835405-5 1994 By monitoring the increase in absorbance at 350 nm after mixing CRBP with IRBP-bound retinol, we measured the amount and time course of retinol transfer from IRBP to CRBP. Vitamin A 85-92 retinol binding protein 3 Homo sapiens 74-78 7835405-5 1994 By monitoring the increase in absorbance at 350 nm after mixing CRBP with IRBP-bound retinol, we measured the amount and time course of retinol transfer from IRBP to CRBP. Vitamin A 85-92 retinol binding protein 3 Homo sapiens 158-162 7835405-5 1994 By monitoring the increase in absorbance at 350 nm after mixing CRBP with IRBP-bound retinol, we measured the amount and time course of retinol transfer from IRBP to CRBP. Vitamin A 136-143 retinol binding protein 3 Homo sapiens 74-78 7835405-5 1994 By monitoring the increase in absorbance at 350 nm after mixing CRBP with IRBP-bound retinol, we measured the amount and time course of retinol transfer from IRBP to CRBP. Vitamin A 136-143 retinol binding protein 3 Homo sapiens 158-162 7835405-7 1994 As determined by measuring the change in absorbance at 350 nm, the mean percentage of IRBP-bound retinol transferred to CRBP was 103 +/- 11% (n = 9). Vitamin A 97-104 retinol binding protein 3 Homo sapiens 86-90 7835405-11 1994 The distribution of retinol between these two proteins was consistent with the nearly 100-fold higher affinity of CRBP for retinol compared with IRBP. Vitamin A 20-27 retinol binding protein 3 Homo sapiens 145-149 8218196-2 1993 The interactions of IRBP with all-trans-retinol have been studied by three fluorescence-based methods and by measurements of binding of 3[H]-labeled all-trans-retinol to this protein. Vitamin A 30-47 retinol binding protein 3 Homo sapiens 20-24 8218196-2 1993 The interactions of IRBP with all-trans-retinol have been studied by three fluorescence-based methods and by measurements of binding of 3[H]-labeled all-trans-retinol to this protein. Vitamin A 34-47 retinol binding protein 3 Homo sapiens 20-24 8218196-3 1993 It was found that IRBP contains two sites with similar but not identical affinities for all-trans-retinol. Vitamin A 91-105 retinol binding protein 3 Homo sapiens 18-22 1987260-1 1991 Interphotoreceptor retinoid binding protein (IRBP) is a soluble glycolipoprotein located between the neurosensory retina and pigment epithelium, which may serve to transport vitamin A derivatives between these tissues. Vitamin A 174-183 retinol binding protein 3 Homo sapiens 0-43 8318167-10 1993 Taken together, the findings provide evidence that, in vivo, IRBP facilitates both the delivery of all-trans retinol to the RPE and the transfer of 11-cis retinal from the RPE to bleached rod photoreceptors, and thereby directly supports the regeneration of rhodopsin in the visual cycle. Vitamin A 109-116 retinol binding protein 3 Homo sapiens 61-65 8144697-8 1993 Release of 11-cis retinal across the apical membrane and re-uptake of retinol from the photoreceptors during the visual cycle is promoted by an intercellular retinoid binding protein (IRBP). Vitamin A 70-77 retinol binding protein 3 Homo sapiens 144-182 8144697-8 1993 Release of 11-cis retinal across the apical membrane and re-uptake of retinol from the photoreceptors during the visual cycle is promoted by an intercellular retinoid binding protein (IRBP). Vitamin A 70-77 retinol binding protein 3 Homo sapiens 184-188 1987260-1 1991 Interphotoreceptor retinoid binding protein (IRBP) is a soluble glycolipoprotein located between the neurosensory retina and pigment epithelium, which may serve to transport vitamin A derivatives between these tissues. Vitamin A 174-183 retinol binding protein 3 Homo sapiens 45-49 2631391-4 1989 In the dark-adapted state, IRBP bound mainly 11-cis retinol and 11-cis retinal in quantities that summed to about 1 mol/mol IRBP. Vitamin A 45-59 retinol binding protein 3 Homo sapiens 27-31 2003123-3 1991 Its extracellular localization and the increased amount of bound all-trans retinol following illumination are consistent with the proposed function of IRBP in extracellular transport or buffering of retinol and retinal isomers in the IPM. Vitamin A 75-82 retinol binding protein 3 Homo sapiens 151-155 2003123-3 1991 Its extracellular localization and the increased amount of bound all-trans retinol following illumination are consistent with the proposed function of IRBP in extracellular transport or buffering of retinol and retinal isomers in the IPM. Vitamin A 199-206 retinol binding protein 3 Homo sapiens 151-155 2265683-3 1990 During delivery the retinol was associated with its putative natural carrier, interphotoreceptor retinoid binding protein (IRBP). Vitamin A 20-27 retinol binding protein 3 Homo sapiens 78-121 2265683-3 1990 During delivery the retinol was associated with its putative natural carrier, interphotoreceptor retinoid binding protein (IRBP). Vitamin A 20-27 retinol binding protein 3 Homo sapiens 123-127 2265683-8 1990 They show that IRBP can serve as a carrier of retinol through an aqueous medium to the RPE, and they illustrate that the visual cycle can be studied in vitro. Vitamin A 46-53 retinol binding protein 3 Homo sapiens 15-19 2910871-0 1989 Mechanism of vitamin A movement between rod outer segments, interphotoreceptor retinoid-binding protein, and liposomes. Vitamin A 13-22 retinol binding protein 3 Homo sapiens 60-103 2910871-4 1989 In the presence of 2 microM IRBP, retinol transfer decreased by approximately one-half, whereas a similar concentration of bovine serum albumin had no effect on this spontaneous transfer. Vitamin A 34-41 retinol binding protein 3 Homo sapiens 28-32 2910871-11 1989 The transfer rate of retinol from IRBP to single unilamellar vesicles also followed first order kinetics with a rate constant of 0.11 s-1 at 22 degrees C, which was approximately 8 times slower than that of transfer between vesicles. Vitamin A 21-28 retinol binding protein 3 Homo sapiens 34-38 2631391-5 1989 After the onset of light-adaptation, all-trans retinol increased from its very low dark-adapted level, peaked at 0.2 mol/mol IRBP and then declined to the dark-adapted level again. Vitamin A 47-54 retinol binding protein 3 Homo sapiens 125-129 2631391-9 1989 These findings support the hypothesis that when rhodopsin is bleached IRBP transports all-trans retinol from the retina to the pigment epithelium and that it delivers 11-cis retinal to the rod outer segments for rhodopsin regeneration. Vitamin A 96-103 retinol binding protein 3 Homo sapiens 70-74 3721783-4 1986 The presence of IRBP in the vitreous was confirmed by Western blot and 3H-retinol binding studies. Vitamin A 74-81 retinol binding protein 3 Homo sapiens 16-20 3093630-6 1986 Frog IRBP was very similar to mammalian IRBP in terms of its immunohistochemical distribution (determined with rabbit anti-frog IRBP antibodies), its molecular weight (sodium dodecyl sulfate polyacrylamide gel electrophoresis and gel-filtration chromatography), retinol- and concanavalin A-binding ability, and because it was synthesized and secreted in vitro by the isolated retina but not by the pigmented layers of eye. Vitamin A 262-269 retinol binding protein 3 Homo sapiens 5-9 4038498-1 1985 Interstitial retinol-binding protein (IRBP) is a soluble glycoprotein present between the retina and pigmented epithelium, which may function to shuttle vitamin A derivatives between these tissues. Vitamin A 153-162 retinol binding protein 3 Homo sapiens 0-36 4038498-1 1985 Interstitial retinol-binding protein (IRBP) is a soluble glycoprotein present between the retina and pigmented epithelium, which may function to shuttle vitamin A derivatives between these tissues. Vitamin A 153-162 retinol binding protein 3 Homo sapiens 38-42 6686234-6 1983 The 146,000 Mr interphotoreceptor retinol-binding protein (IRBP) may function in extracellular retinol transport in the IPS. Vitamin A 34-41 retinol binding protein 3 Homo sapiens 59-63 6543481-8 1984 The amount of retinol bound to IRBP increased if the eyes were exposed to light, when it was estimated that the protein carried up to 30% of its full capacity for all-trans retinol. Vitamin A 14-21 retinol binding protein 3 Homo sapiens 31-35 6543481-8 1984 The amount of retinol bound to IRBP increased if the eyes were exposed to light, when it was estimated that the protein carried up to 30% of its full capacity for all-trans retinol. Vitamin A 173-180 retinol binding protein 3 Homo sapiens 31-35 6543481-9 1984 In addition to all-trans retinol, IRBP carried smaller amounts of 11-cis retinol. Vitamin A 66-80 retinol binding protein 3 Homo sapiens 34-38 6543481-10 1984 The proportion of 11-cis retinol was frequently higher in eyes that had been protected from illumination, suggesting that IRBP plays a role in rhodopsin regeneration during dark-adaptation. Vitamin A 18-32 retinol binding protein 3 Homo sapiens 122-126 25565073-3 2015 Within the interphotoreceptor matrix, all-trans retinol, 11-cis retinol and retinal are bound by interphotoreceptor retinoid-binding protein (IRBP). Vitamin A 48-55 retinol binding protein 3 Homo sapiens 97-140 25565073-3 2015 Within the interphotoreceptor matrix, all-trans retinol, 11-cis retinol and retinal are bound by interphotoreceptor retinoid-binding protein (IRBP). Vitamin A 48-55 retinol binding protein 3 Homo sapiens 142-146 25565073-5 2015 HPLC was used to evaluate the ability of IRBP to protect all-trans and 11-cis retinols from photodegradation when exposed to incandescent light (0 to 8842 muW cm(-2)); time periods of 0-60 min, and bIRBP: retinol molar ratios of 1:1 to 1:5. Vitamin A 78-86 retinol binding protein 3 Homo sapiens 41-45 21730941-13 2011 All-trans retinol is then transferred out of the outer segment and into neighboring cells by the specialized carrier Interphotoreceptor Retinoid Binding Protein (IRBP). Vitamin A 10-17 retinol binding protein 3 Homo sapiens 117-160 21730941-13 2011 All-trans retinol is then transferred out of the outer segment and into neighboring cells by the specialized carrier Interphotoreceptor Retinoid Binding Protein (IRBP). Vitamin A 10-17 retinol binding protein 3 Homo sapiens 162-166