PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 23688744-2 2013 Vitamin A storage within the HSCs is achieved through the cooperative action of two proteins, cellular retinol-binding protein (CRBP) I and lecithin:retinol acyltransferase (LRAT). Vitamin A 0-9 retinol binding protein 1 Homo sapiens 128-135 23407361-9 2013 The third technique is real-time analysis of STRA6-catalyzed retinol transport from holo-RBP to cellular retinol binding protein I (CRBP-I). Vitamin A 61-68 retinol binding protein 1 Homo sapiens 96-130 23362116-1 2013 Cellular retinol-binding protein, type I (CrbpI), encoded by retinol-binding protein, type 1 (Rbp1), is a chaperone of vitamin A (retinol) that is epigenetically silenced in ~25% of human breast cancers. Vitamin A 9-16 retinol binding protein 1 Homo sapiens 42-47 23362116-1 2013 Cellular retinol-binding protein, type I (CrbpI), encoded by retinol-binding protein, type 1 (Rbp1), is a chaperone of vitamin A (retinol) that is epigenetically silenced in ~25% of human breast cancers. Vitamin A 9-16 retinol binding protein 1 Homo sapiens 61-92 23362116-1 2013 Cellular retinol-binding protein, type I (CrbpI), encoded by retinol-binding protein, type 1 (Rbp1), is a chaperone of vitamin A (retinol) that is epigenetically silenced in ~25% of human breast cancers. Vitamin A 9-16 retinol binding protein 1 Homo sapiens 94-98 23362116-1 2013 Cellular retinol-binding protein, type I (CrbpI), encoded by retinol-binding protein, type 1 (Rbp1), is a chaperone of vitamin A (retinol) that is epigenetically silenced in ~25% of human breast cancers. Vitamin A 119-128 retinol binding protein 1 Homo sapiens 42-47 23362116-1 2013 Cellular retinol-binding protein, type I (CrbpI), encoded by retinol-binding protein, type 1 (Rbp1), is a chaperone of vitamin A (retinol) that is epigenetically silenced in ~25% of human breast cancers. Vitamin A 119-128 retinol binding protein 1 Homo sapiens 61-92 23362116-1 2013 Cellular retinol-binding protein, type I (CrbpI), encoded by retinol-binding protein, type 1 (Rbp1), is a chaperone of vitamin A (retinol) that is epigenetically silenced in ~25% of human breast cancers. Vitamin A 119-128 retinol binding protein 1 Homo sapiens 94-98 23362116-1 2013 Cellular retinol-binding protein, type I (CrbpI), encoded by retinol-binding protein, type 1 (Rbp1), is a chaperone of vitamin A (retinol) that is epigenetically silenced in ~25% of human breast cancers. Vitamin A 61-68 retinol binding protein 1 Homo sapiens 42-47 23362116-1 2013 Cellular retinol-binding protein, type I (CrbpI), encoded by retinol-binding protein, type 1 (Rbp1), is a chaperone of vitamin A (retinol) that is epigenetically silenced in ~25% of human breast cancers. Vitamin A 61-68 retinol binding protein 1 Homo sapiens 94-98 23220004-7 2013 The relative contribution of each enzyme type in retinoid metabolism is discussed in terms of the different subcellular localization, topology of membrane-bound enzymes, kinetic constants, binding affinity of CRBP for retinol and retinaldehyde, and partition of retinoid pools between membranes and cytoplasm. Vitamin A 218-225 retinol binding protein 1 Homo sapiens 209-213 23407361-9 2013 The third technique is real-time analysis of STRA6-catalyzed retinol transport from holo-RBP to cellular retinol binding protein I (CRBP-I). Vitamin A 61-68 retinol binding protein 1 Homo sapiens 132-138 22665496-3 2012 Instead, activation of the receptor is triggered by STRA6-mediated translocation of retinol from serum RBP to an intracellular acceptor, the retinol-binding protein CRBP-I. Vitamin A 84-91 retinol binding protein 1 Homo sapiens 165-171 23155051-9 2013 Colocalization of Rdh10, Crbp1, and LRAT on lipid droplets suggests a metabolon that mediates retinol homeostasis. Vitamin A 94-101 retinol binding protein 1 Homo sapiens 25-30 22815070-9 2012 We also found that STRA6 catalyzes efficient retinol exchange between intracellular CRBP-I and extracellular RBP, even in the presence of holo-RBP. Vitamin A 45-52 retinol binding protein 1 Homo sapiens 84-90 22815070-6 2012 We show here that STRA6 can couple strongly to both CRBP-I and CRBP-II for retinol efflux to apo-RBP. Vitamin A 75-82 retinol binding protein 1 Homo sapiens 52-58 22815070-7 2012 Strikingly, pure apo-RBP can cause almost complete depletion of retinol taken up by CRBP-I in a STRA6-dependent manner. Vitamin A 64-71 retinol binding protein 1 Homo sapiens 84-90 22665496-3 2012 Instead, activation of the receptor is triggered by STRA6-mediated translocation of retinol from serum RBP to an intracellular acceptor, the retinol-binding protein CRBP-I. Vitamin A 141-148 retinol binding protein 1 Homo sapiens 165-171 22665496-4 2012 The observations also demonstrate that the movement of retinol from RBP to CRBP-I, and thus activation of STRA6, is critically linked to the intracellular metabolism of the vitamin. Vitamin A 55-62 retinol binding protein 1 Homo sapiens 75-81 22230368-3 2012 CRBP1 (cellular retinol binding protein 1), a key component of retinoid signaling pathway, is known to take part in vitamin A metabolism and intracellular transporting of retinoids. Vitamin A 116-125 retinol binding protein 1 Homo sapiens 0-5 22230368-3 2012 CRBP1 (cellular retinol binding protein 1), a key component of retinoid signaling pathway, is known to take part in vitamin A metabolism and intracellular transporting of retinoids. Vitamin A 116-125 retinol binding protein 1 Homo sapiens 7-41 22171153-7 2011 We selected the following single-nucleotide polymorphisms (SNPs) in genes related to vitamin A and vitamin C concentrations: rs176990 and rs190910 in the retinol-binding protein 1 (RBP1) gene; and rs10063949 and rs1279683 in the Na+-dependent L-ascorbic acid transporters 1 and 2, respectively (encoded by the SLC23A1 and SLC23A2 genes). Vitamin A 85-94 retinol binding protein 1 Homo sapiens 154-179 21514413-6 2011 Aberrantly expressed RA signaling molecules included i) the retinol-binding protein CRBP1, which facilitates cellular retinoid uptake; ii) ALDH1A1, capable of activating RA precursors; iii) the RA-degrading enzyme CYP26B1; and iv) the RA-binding protein FABP5, which can inhibit RA-induced differentiation. Vitamin A 60-67 retinol binding protein 1 Homo sapiens 84-89 21621639-10 2012 The ratio apo-CRBP1/holo-CRBP1 participates by influencing retinol flux into and out of storage as retinyl esters, thereby modulating substrate to support atRA biosynthesis. Vitamin A 59-66 retinol binding protein 1 Homo sapiens 14-19 21621639-10 2012 The ratio apo-CRBP1/holo-CRBP1 participates by influencing retinol flux into and out of storage as retinyl esters, thereby modulating substrate to support atRA biosynthesis. Vitamin A 59-66 retinol binding protein 1 Homo sapiens 25-30 21382444-1 2011 BACKGROUND: Cellular retinol binding-protein I (CRBPI) and cellular retinol binding-protein II (CRBPII) serve as intracellular retinoid chaperones that bind retinol and retinal with high affinity and facilitate substrate delivery to select enzymes that catalyze retinoic acid (RA) and retinyl ester biosynthesis. Vitamin A 21-28 retinol binding protein 1 Homo sapiens 48-53 22171153-7 2011 We selected the following single-nucleotide polymorphisms (SNPs) in genes related to vitamin A and vitamin C concentrations: rs176990 and rs190910 in the retinol-binding protein 1 (RBP1) gene; and rs10063949 and rs1279683 in the Na+-dependent L-ascorbic acid transporters 1 and 2, respectively (encoded by the SLC23A1 and SLC23A2 genes). Vitamin A 85-94 retinol binding protein 1 Homo sapiens 181-185 18343808-5 2008 RA and retinol also regulate expression of ADH1, cellular retinol binding protein 1 and cellular RA binding protein 2 in fibroid and myometrial cells. Vitamin A 7-14 retinol binding protein 1 Homo sapiens 49-83 19965581-1 2010 The main retinol carriers in the cytosol are the cellular retinol-binding proteins types I and II (CRBP-I and CRBP-II), which exhibit distinct tissue distributions. Vitamin A 9-16 retinol binding protein 1 Homo sapiens 99-105 19965581-1 2010 The main retinol carriers in the cytosol are the cellular retinol-binding proteins types I and II (CRBP-I and CRBP-II), which exhibit distinct tissue distributions. Vitamin A 58-65 retinol binding protein 1 Homo sapiens 99-105 19110304-8 2009 CONCLUSIONS: The impaired conversion of retinol to RA in ovarian cancer cells and decreased CRBP1 protein expression in prophylactic oophorectomies support our hypothesis that concomitant losses of vitamin A metabolism and CRBP1 expression contribute to ovarian oncogenesis. Vitamin A 198-207 retinol binding protein 1 Homo sapiens 92-97 18569334-6 2008 is capable to oxidize all-trans-retinol bound to CRBP (holo-CRBP) to all-trans-retinaldehyde and then to all-trans-retinoic acid. Vitamin A 22-39 retinol binding protein 1 Homo sapiens 49-53 18569334-6 2008 is capable to oxidize all-trans-retinol bound to CRBP (holo-CRBP) to all-trans-retinaldehyde and then to all-trans-retinoic acid. Vitamin A 22-39 retinol binding protein 1 Homo sapiens 60-64 21042705-3 2010 As CRBP1 regulates intracellular retinoic acid (vitamin A) homeostasis, which is involved in morphogenesis, and cellular proliferation and differentiation, the loss of CRBP1 could cause tumorigenesis in BC. Vitamin A 48-57 retinol binding protein 1 Homo sapiens 3-8 21042705-3 2010 As CRBP1 regulates intracellular retinoic acid (vitamin A) homeostasis, which is involved in morphogenesis, and cellular proliferation and differentiation, the loss of CRBP1 could cause tumorigenesis in BC. Vitamin A 48-57 retinol binding protein 1 Homo sapiens 168-173 21067480-3 2010 RGR, encoding the retinal pigment epithelium (RPE) G protein-coupled receptor acting in vitro as a photoisomerase; RBP1, encoding the ubiquitous cellular retinol binding protein carrying intracellular all-trans retinoids; RBP3, encoding the interphotoreceptor retinoid binding protein, a retinal-specific protein which shuttles all-trans retinol from photoreceptors to RPE and 11-cis retinal from RPE to photoreceptors. Vitamin A 154-161 retinol binding protein 1 Homo sapiens 115-119 19965581-4 2010 The remarkable difference in intrinsic stability between the two homologs appears to modulate their binding properties: the stronger retinol binder CRBP-I displays a reduced flexibility of the backbone structure with respect to CRBP-II. Vitamin A 133-140 retinol binding protein 1 Homo sapiens 148-154 19110304-1 2009 OBJECTIVES: We have previously reported that loss in expression of a protein considered critical for vitamin A homeostasis, cellular retinol-binding protein 1 (CRBP1), is an early event in ovarian carcinogenesis. Vitamin A 101-110 retinol binding protein 1 Homo sapiens 124-158 19110304-1 2009 OBJECTIVES: We have previously reported that loss in expression of a protein considered critical for vitamin A homeostasis, cellular retinol-binding protein 1 (CRBP1), is an early event in ovarian carcinogenesis. Vitamin A 101-110 retinol binding protein 1 Homo sapiens 160-165 19250215-2 2009 In a previous study we indicated that xanthine dehydrogenase (XDH) is able to oxidize actively both all-trans-retinol (t-ROL) bound to the CRBP (holo-CRBP) and all-trans-retinaldehyde (t-RAL) to all-trans-retinoic acid (t-RA) in human mammary epithelial cells (HMEC). Vitamin A 100-117 retinol binding protein 1 Homo sapiens 139-143 19250215-2 2009 In a previous study we indicated that xanthine dehydrogenase (XDH) is able to oxidize actively both all-trans-retinol (t-ROL) bound to the CRBP (holo-CRBP) and all-trans-retinaldehyde (t-RAL) to all-trans-retinoic acid (t-RA) in human mammary epithelial cells (HMEC). Vitamin A 100-117 retinol binding protein 1 Homo sapiens 150-154 19250215-2 2009 In a previous study we indicated that xanthine dehydrogenase (XDH) is able to oxidize actively both all-trans-retinol (t-ROL) bound to the CRBP (holo-CRBP) and all-trans-retinaldehyde (t-RAL) to all-trans-retinoic acid (t-RA) in human mammary epithelial cells (HMEC). Vitamin A 119-124 retinol binding protein 1 Homo sapiens 139-143 19250215-2 2009 In a previous study we indicated that xanthine dehydrogenase (XDH) is able to oxidize actively both all-trans-retinol (t-ROL) bound to the CRBP (holo-CRBP) and all-trans-retinaldehyde (t-RAL) to all-trans-retinoic acid (t-RA) in human mammary epithelial cells (HMEC). Vitamin A 119-124 retinol binding protein 1 Homo sapiens 150-154 17991731-1 2008 This laboratory has advanced a model whereby retinol is transported around the body bound to retinol-binding protein (RBP), is transferred across the membrane of cells by a specific receptor/transporter, and is picked up from the membrane by an intracellular homolog, cellular retinol-binding protein (CRBP). Vitamin A 45-52 retinol binding protein 1 Homo sapiens 302-306 18689151-4 2008 Their effects are mediated through nuclear receptors called Retinoic Acid Receptor (RAR) and regulated by molecules such as Cellular Retinol-Binding Protein 1 (CRBP1) that function in retinol storage. Vitamin A 184-191 retinol binding protein 1 Homo sapiens 124-158 18689151-4 2008 Their effects are mediated through nuclear receptors called Retinoic Acid Receptor (RAR) and regulated by molecules such as Cellular Retinol-Binding Protein 1 (CRBP1) that function in retinol storage. Vitamin A 184-191 retinol binding protein 1 Homo sapiens 160-165 16787387-3 2006 Estimation of the relative contribution of enzymes of each type was difficult since kinetics were performed with different methodologies, but SDRs would supposedly play a major role because of their low K(m) values, and because they were found to be active with retinol bound to CRBPI (cellular retinol binding protein type I). Vitamin A 262-269 retinol binding protein 1 Homo sapiens 279-284 18181245-1 2008 To bind and release its ligand, cellular retinol-binding protein type I (CRBP) needs to undergo conformational and dynamic changes to connect the inner, solvent-shielded cavity, where retinol is found to bind, and the outside medium. Vitamin A 41-48 retinol binding protein 1 Homo sapiens 73-77 17681135-7 2007 Accordingly, we found that Notch1 signaling is linked to vitamin A metabolism by regulating the expression of cellular retinol binding protein 1 (CRBP1), required to generate a pool of intracellular retinol. Vitamin A 57-66 retinol binding protein 1 Homo sapiens 110-144 17681135-7 2007 Accordingly, we found that Notch1 signaling is linked to vitamin A metabolism by regulating the expression of cellular retinol binding protein 1 (CRBP1), required to generate a pool of intracellular retinol. Vitamin A 57-66 retinol binding protein 1 Homo sapiens 146-151 17681135-7 2007 Accordingly, we found that Notch1 signaling is linked to vitamin A metabolism by regulating the expression of cellular retinol binding protein 1 (CRBP1), required to generate a pool of intracellular retinol. Vitamin A 119-126 retinol binding protein 1 Homo sapiens 146-151 16787387-3 2006 Estimation of the relative contribution of enzymes of each type was difficult since kinetics were performed with different methodologies, but SDRs would supposedly play a major role because of their low K(m) values, and because they were found to be active with retinol bound to CRBPI (cellular retinol binding protein type I). Vitamin A 262-269 retinol binding protein 1 Homo sapiens 286-325 16787387-5 2006 Our results demonstrate that none of the enzymes, including the SDR members, are active with CRBPI-bound retinoids, which questions the previously suggested role of CRBPI as a retinol supplier in the retinoic acid synthesis pathway. Vitamin A 176-183 retinol binding protein 1 Homo sapiens 165-170 15826155-1 2005 We have calculated proton chemical shift perturbations (CSPs) of retinol in the cellular retinol-binding protein (CRBP) through the use of a recently developed computational approach (Wang et al. Vitamin A 65-72 retinol binding protein 1 Homo sapiens 80-112 15865448-7 2005 As a result, the widely expressed CRBPI, which binds all-trans-retinol with much higher affinity than all-trans-retinaldehyde, restricts the oxidation of all-trans-retinol by RDH12, but has little effect on the reduction of all-trans-retinaldehyde, and CRALBP inhibits the reduction of 11-cis-retinal stronger than the oxidation of 11-cis-retinol, in accord with its higher affinity for 11-cis-retinal. Vitamin A 62-70 retinol binding protein 1 Homo sapiens 34-39 15865448-7 2005 As a result, the widely expressed CRBPI, which binds all-trans-retinol with much higher affinity than all-trans-retinaldehyde, restricts the oxidation of all-trans-retinol by RDH12, but has little effect on the reduction of all-trans-retinaldehyde, and CRALBP inhibits the reduction of 11-cis-retinal stronger than the oxidation of 11-cis-retinol, in accord with its higher affinity for 11-cis-retinal. Vitamin A 332-346 retinol binding protein 1 Homo sapiens 34-39 16866541-4 2006 We have investigated the mechanism of retinol uptake by the cellular retinol-binding protein type I (CRBP) using line shape analysis of NMR signals. Vitamin A 38-45 retinol binding protein 1 Homo sapiens 101-105 16866541-5 2006 The highly similar structures of apo- and holo-CRBP exhibit closed conformations that seemingly offer no access to ligand, yet the protein binds retinol rapidly and with high affinity. Vitamin A 145-152 retinol binding protein 1 Homo sapiens 47-51 16866541-7 2006 An initial nonspecific encounter with the ligand induces the formation of long-lived conformers in the portal region of CRBP suggesting a mechanism how retinol accesses the cavity. Vitamin A 152-159 retinol binding protein 1 Homo sapiens 120-124 16575402-1 2006 Cellular retinol binding protein-1 (CRBP-1) contributes to the maintenance of the differentiative state of endometrial glandular cells through the regulation of bioavailability of retinol and derivatives, but its role in endometrial oncogenetic process remains unclear. Vitamin A 9-16 retinol binding protein 1 Homo sapiens 36-42 16575402-15 2006 The CRBP-1 loss during the progression of endometrial cancer suggests a new potential target for pharmacological strategies aimed to counteract its progression by increased intracellular retinol bioavailability. Vitamin A 187-194 retinol binding protein 1 Homo sapiens 4-10 16150442-8 2005 We also demonstrate that overexpression of CRBPI can drive the accumulation of intracellular retinol from unbound retinol added to the medium. Vitamin A 93-100 retinol binding protein 1 Homo sapiens 43-48 16150442-8 2005 We also demonstrate that overexpression of CRBPI can drive the accumulation of intracellular retinol from unbound retinol added to the medium. Vitamin A 114-121 retinol binding protein 1 Homo sapiens 43-48 15865448-7 2005 As a result, the widely expressed CRBPI, which binds all-trans-retinol with much higher affinity than all-trans-retinaldehyde, restricts the oxidation of all-trans-retinol by RDH12, but has little effect on the reduction of all-trans-retinaldehyde, and CRALBP inhibits the reduction of 11-cis-retinal stronger than the oxidation of 11-cis-retinol, in accord with its higher affinity for 11-cis-retinal. Vitamin A 56-70 retinol binding protein 1 Homo sapiens 34-39 15826155-1 2005 We have calculated proton chemical shift perturbations (CSPs) of retinol in the cellular retinol-binding protein (CRBP) through the use of a recently developed computational approach (Wang et al. Vitamin A 65-72 retinol binding protein 1 Homo sapiens 114-118 15500655-1 2004 AIMS: Cellular retinol-binding protein-1 (CRBP-1) contributes to the maintenance of the differentiated state of the endometrium through retinol bioavailability regulation. Vitamin A 15-22 retinol binding protein 1 Homo sapiens 42-48 15632377-2 2005 Cellular retinol-binding protein I (CRBP-I) functions in retinol storage and its expression is lower in human cancers than in normal cells. Vitamin A 9-16 retinol binding protein 1 Homo sapiens 36-42 14704332-2 2004 Lecithin:retinol acyltransferase (LRAT), a microsomal enzyme present in liver and several other retinol-metabolizing tissues, esterifies retinol that is associated with a cellular retinol-binding protein, CRBP or CRBP-II. Vitamin A 96-103 retinol binding protein 1 Homo sapiens 205-209 15094224-1 2004 BACKGROUND/AIMS: Cellular retinol-binding protein-1 (CRBP-1) which is involved in vitamin A metabolism is highly expressed in liver cells, particularly in hepatic stellate cells (HSCs). Vitamin A 82-91 retinol binding protein 1 Homo sapiens 17-51 15094224-1 2004 BACKGROUND/AIMS: Cellular retinol-binding protein-1 (CRBP-1) which is involved in vitamin A metabolism is highly expressed in liver cells, particularly in hepatic stellate cells (HSCs). Vitamin A 82-91 retinol binding protein 1 Homo sapiens 53-59 14704332-2 2004 Lecithin:retinol acyltransferase (LRAT), a microsomal enzyme present in liver and several other retinol-metabolizing tissues, esterifies retinol that is associated with a cellular retinol-binding protein, CRBP or CRBP-II. Vitamin A 9-16 retinol binding protein 1 Homo sapiens 205-209 14704332-2 2004 Lecithin:retinol acyltransferase (LRAT), a microsomal enzyme present in liver and several other retinol-metabolizing tissues, esterifies retinol that is associated with a cellular retinol-binding protein, CRBP or CRBP-II. Vitamin A 96-103 retinol binding protein 1 Homo sapiens 205-209 12883492-1 2003 The cellular retinol-binding protein-1 (CRBP-1) plays a key role in the esterification and intercellular transfer of retinol. Vitamin A 13-20 retinol binding protein 1 Homo sapiens 40-46 12883492-10 2003 In conclusion, our findings demonstrate that CRBP-1 detection may be useful for the discrimination between nonneoplastic and neoplastic liver cells and suggest that modulation of CRBP-1 expression in HCCs contributes to tumor growth and progression via retinoid-mediated signaling and disruption of cellular vitamin A homeostasis. Vitamin A 308-317 retinol binding protein 1 Homo sapiens 45-51 12883492-10 2003 In conclusion, our findings demonstrate that CRBP-1 detection may be useful for the discrimination between nonneoplastic and neoplastic liver cells and suggest that modulation of CRBP-1 expression in HCCs contributes to tumor growth and progression via retinoid-mediated signaling and disruption of cellular vitamin A homeostasis. Vitamin A 308-317 retinol binding protein 1 Homo sapiens 179-185 12631600-1 2003 PURPOSE: We have previously found that cellular retinol-binding protein 1 (CRBP1),involved in retinol transport and metabolism, is down-regulated in an in vitro rat model of ovarian cancer and in several human ovarian cancer cell lines. Vitamin A 48-55 retinol binding protein 1 Homo sapiens 75-80 12631600-11 2003 Our findings suggest that the loss of CRBP1 expression contributes to the ovarian cancer oncogenesis via altered vitamin A metabolism. Vitamin A 113-122 retinol binding protein 1 Homo sapiens 38-43 12618084-6 2003 In the prevailing oxidative reaction it recognizes both free- and CRBP-bound retinol, and shows preference toward NADP as a co-substrate. Vitamin A 77-84 retinol binding protein 1 Homo sapiens 66-70 12534290-0 2003 Differential recognition of the free versus bound retinol by human microsomal retinol/sterol dehydrogenases: characterization of the holo-CRBP dehydrogenase activity of RoDH-4. Vitamin A 50-57 retinol binding protein 1 Homo sapiens 138-142 12534290-2 2003 In the cytosol of various cells, most retinol exists in a bound form, complexed with cellular retinol binding protein type I (holo-CRBP). Vitamin A 38-45 retinol binding protein 1 Homo sapiens 131-135 12534290-2 2003 In the cytosol of various cells, most retinol exists in a bound form, complexed with cellular retinol binding protein type I (holo-CRBP). Vitamin A 94-101 retinol binding protein 1 Homo sapiens 131-135 12534290-5 2003 The ability to utilize holo-CRBP differentiates RoDH-4 from a related enzyme, RoDH-like 3alpha-hydroxysteroid dehydrogenase (3alpha-HSD), which is 3-fold more active with free retinol than RoDH-4 but is 15-fold less active toward holo-CRBP. Vitamin A 176-183 retinol binding protein 1 Homo sapiens 28-32 12534290-10 2003 The results of this study suggest that the human retinol-active SDRs are not functionally equivalent and that, in contrast to RoDH-like 3alpha-HSD, RoDH-4 can access the bound form of retinol for retinoic acid production and is regulated by the apo-/holo-CRBP ratio. Vitamin A 184-191 retinol binding protein 1 Homo sapiens 255-259 14673826-1 2003 The acid-induced denaturation of cellular retinol-binding proteins types I and II (CRBP I and II), in the presence and in the absence of the ligand, was studied by electrospray ionization mass spectrometry (ESI-MS) in the pH range 6.9-2.4. Vitamin A 42-49 retinol binding protein 1 Homo sapiens 83-96 12438255-11 2002 In addition, retinol-related genes such as CRABP2 and retinol-binding protein 1 were overexpressed in WT, and CRABP2 was more highly expressed in the poor outcome patients, which suggests that retinoid acid may be a potential drug. Vitamin A 13-20 retinol binding protein 1 Homo sapiens 54-79 11704871-4 2001 Ectopic CRBP-mediated inhibition of anchorage-independent cell survival and colony formation in the absence of significantly altered responses to either retinol or retinoic acid was also documented in T47D human breast cancer cells. Vitamin A 153-160 retinol binding protein 1 Homo sapiens 8-12 12034496-1 2002 Members of the cellular retinoic acid (CRABP) and retinol binding (CRBP) proteins family are involved in the metabolic pathways of retinoic acid (RA) and retinal respectively. Vitamin A 50-57 retinol binding protein 1 Homo sapiens 67-71 12177003-3 2002 Retinol binding to CRBP IV leads to an absorption spectrum distinct from a typical holo-CRBP spectrum and is characterized by an affinity (K(d) = approximately 200 nm) lower than those for CRBP I, II, and III, as established in direct and competitive binding assays. Vitamin A 0-7 retinol binding protein 1 Homo sapiens 19-23 12177003-3 2002 Retinol binding to CRBP IV leads to an absorption spectrum distinct from a typical holo-CRBP spectrum and is characterized by an affinity (K(d) = approximately 200 nm) lower than those for CRBP I, II, and III, as established in direct and competitive binding assays. Vitamin A 0-7 retinol binding protein 1 Homo sapiens 88-92 12177003-3 2002 Retinol binding to CRBP IV leads to an absorption spectrum distinct from a typical holo-CRBP spectrum and is characterized by an affinity (K(d) = approximately 200 nm) lower than those for CRBP I, II, and III, as established in direct and competitive binding assays. Vitamin A 0-7 retinol binding protein 1 Homo sapiens 189-208 12177003-7 2002 These results indicate that human CRBP IV belongs to a clearly distinct CRBP subfamily and suggest a relatively different mode of retinol binding for this binding protein. Vitamin A 130-137 retinol binding protein 1 Homo sapiens 34-38 11879576-4 2002 Of particular interest, cellular retinol-binding protein 1 (CRBP1) and retinol-binding protein (RBP), two genes whose products are involved in retinol transport and metabolism, were found to be downregulated in this ovarian cancer model system. Vitamin A 33-40 retinol binding protein 1 Homo sapiens 60-65 11879576-6 2002 We hypothesize that the loss of CRBP1 and RBP expression disrupts retinol metabolism and retinoic acid production, which may facilitate the occurrence of genetic damage leading to the malignant transformation of the ovarian surface epithelium, the cells from which ovarian cancer arises. Vitamin A 66-73 retinol binding protein 1 Homo sapiens 32-37 10852718-5 2000 We also measured binding of FA to a retinoic acid (CRABP-I) and a retinol (CRBP-II) binding protein and we have extended to 19 different FA our characterization of the FA-ADIFAB and FA-rat intestinal FABP interactions. Vitamin A 66-73 retinol binding protein 1 Homo sapiens 75-79 11274389-1 2001 Two cellular retinol-binding proteins (CRBP I and II) with distinct tissue distributions and retinoid-binding properties have been recognized thus far in mammals. Vitamin A 13-20 retinol binding protein 1 Homo sapiens 39-52 11274389-9 2001 As revealed by a 2.3-A x-ray molecular model of apo-CRBP III, the amino acid residues that line the retinol-binding site in CRBP I and II are positioned nearly identically in the structure of CRBP III. Vitamin A 100-107 retinol binding protein 1 Homo sapiens 124-137 10725366-2 2000 This protein is related to cellular retinol-binding protein type I (CRBP I) but has 3,4-didehydroretinol, rather than retinol, as a ligand. Vitamin A 36-43 retinol binding protein 1 Homo sapiens 68-74 10716965-2 2000 The cellular retinol-binding protein (CRBP) binds vitamin A with high affinity and is postulated to regulate its uptake and metabolism. Vitamin A 50-59 retinol binding protein 1 Homo sapiens 38-42 10716965-11 2000 CONCLUSION: CRBP is underexpressed in 24% (95% confidence interval = 12.5%-36.5%) of human breast carcinomas, implying a link between cellular vitamin A homeostasis and breast cancer. Vitamin A 143-152 retinol binding protein 1 Homo sapiens 12-16 10716965-12 2000 We hypothesize that the loss of CRBP restricts the effects of endogenous vitamin A on breast epithelial cells. Vitamin A 73-82 retinol binding protein 1 Homo sapiens 32-36 10725366-2 2000 This protein is related to cellular retinol-binding protein type I (CRBP I) but has 3,4-didehydroretinol, rather than retinol, as a ligand. Vitamin A 97-104 retinol binding protein 1 Homo sapiens 68-74 10460195-0 1999 Vitamin A-sensitive tissues in transgenic mice expressing high levels of human cellular retinol-binding protein type I are not altered phenotypically. Vitamin A 0-9 retinol binding protein 1 Homo sapiens 79-118 10460195-3 1999 Thus, high expression of human CRBP(I) [hCRBP(I)] in transgenic mice might be expected to increase the production of retinoic acid in tissues, thereby inducing a phenotype resembling vitamin A toxicity. Vitamin A 183-192 retinol binding protein 1 Homo sapiens 31-38 10460195-3 1999 Thus, high expression of human CRBP(I) [hCRBP(I)] in transgenic mice might be expected to increase the production of retinoic acid in tissues, thereby inducing a phenotype resembling vitamin A toxicity. Vitamin A 183-192 retinol binding protein 1 Homo sapiens 40-48 10460195-4 1999 Alternatively, a vitamin A-deficient phenotype could also be envisioned as a result of an increased accumulation of vitamin A in storage cells induced by a high hCRBP(I) level. Vitamin A 17-26 retinol binding protein 1 Homo sapiens 161-169 10421060-4 1999 Similarly, the concentrations of cellular retinol-binding protein and cellular retinoic acid-binding protein (CRBPI and CRABPII, respectively) were 10-30 times lower in HaCaT cells than in HEK corresponding to a reduced mRNA expression of these proteins. Vitamin A 42-49 retinol binding protein 1 Homo sapiens 110-115 10419811-8 1999 Moreover, binding and possibly also metabolism of retinol may occur in the CRBP I positive villous stromal cells and decidual cells of the basal plate. Vitamin A 50-57 retinol binding protein 1 Homo sapiens 75-81 10092641-1 1999 Cellular retinol-binding proteins types I and II (CRBP-I and CRBP-II) are known to differentially facilitate retinoid metabolism by several membrane-associated enzymes. Vitamin A 9-16 retinol binding protein 1 Homo sapiens 50-56 10209249-2 1999 Immunohistochemistry was used to localise the cellular retinoid binding-proteins for retinol (CRBP I) and retinoic acid (CRABP I) in the embryonic and adult olfactory system. Vitamin A 85-92 retinol binding protein 1 Homo sapiens 94-100 10329026-6 1999 Several retinol dehydrogenase (RDH) enzymes, members of the short-chain dehydrogenase/reductase (SDR) gene superfamily, catalyze the first and rate-limiting step that generates retinaldehyde from retinol bound to cellular retinol-binding protein (holo-CRBP). Vitamin A 8-15 retinol binding protein 1 Homo sapiens 252-256 10329026-10 1999 It encodes a protein that, using NAD+ as a preferred cofactor, utilizes free and CRBP-bound all-trans-retinol and steroids as substrates. Vitamin A 92-109 retinol binding protein 1 Homo sapiens 81-85 10092641-4 1999 The results showed that ligand transfer of retinol from CRBP-I was >5-fold faster than transfer from CRBP-II. Vitamin A 43-50 retinol binding protein 1 Homo sapiens 56-62 10026291-1 1999 Microsomal enzymes that catalyze the first step in the biosynthesis of retinoic acid from retinal, retinol dehydrogenases (RDHs), access retinol bound to cellular retinol-binding protein (CRBP). Vitamin A 99-106 retinol binding protein 1 Homo sapiens 154-186 10026291-1 1999 Microsomal enzymes that catalyze the first step in the biosynthesis of retinoic acid from retinal, retinol dehydrogenases (RDHs), access retinol bound to cellular retinol-binding protein (CRBP). Vitamin A 99-106 retinol binding protein 1 Homo sapiens 188-192 10026291-9 1999 For example, L35 mutants had lower kcat values than wild-type CRBP; thus, L35 seems important for RDH access to retinol. Vitamin A 112-119 retinol binding protein 1 Homo sapiens 62-66 10026291-11 1999 These results suggest a role for the helical cap region as a locus for RDH interaction and as a portal for ligand access to CRBP, and show that the affinity (Kd) of CRBP for retinol alone does not determine the efficiency of holo-CRBP as substrate. Vitamin A 174-181 retinol binding protein 1 Homo sapiens 165-169 10026291-11 1999 These results suggest a role for the helical cap region as a locus for RDH interaction and as a portal for ligand access to CRBP, and show that the affinity (Kd) of CRBP for retinol alone does not determine the efficiency of holo-CRBP as substrate. Vitamin A 174-181 retinol binding protein 1 Homo sapiens 165-169 7835405-0 1994 Exchange of retinol between IRBP and CRBP. Vitamin A 12-19 retinol binding protein 1 Homo sapiens 37-41 10506831-2 1999 One of these proteins, the widely expressed (throughout retinoid target tissues and in all vertebrates) and highly conserved cellular retinol-binding protein (CRBP), sequesters retinol in an internal binding pocket that segregates it from the intracellular milieu. Vitamin A 134-141 retinol binding protein 1 Homo sapiens 159-163 10506831-3 1999 The CRBP-retinol complex appears to be the quantitatively major form of retinol in vivo, and may protect the promiscuous substrate from nonenzymatic degradation and/or non-specific enzymes. Vitamin A 9-16 retinol binding protein 1 Homo sapiens 4-8 10506831-3 1999 The CRBP-retinol complex appears to be the quantitatively major form of retinol in vivo, and may protect the promiscuous substrate from nonenzymatic degradation and/or non-specific enzymes. Vitamin A 72-79 retinol binding protein 1 Homo sapiens 4-8 10506831-5 1999 But only a fraction of these (some of the short-chain de-hydrogenases/reductases) have the fascinating additional ability of catalyzing retinal synthesis from CRBP-bound retinol as well. Vitamin A 170-177 retinol binding protein 1 Homo sapiens 159-163 9705855-4 1998 In the cells, most all-trans retinol is bound to cellular retinol binding protein (CRBP). Vitamin A 29-36 retinol binding protein 1 Homo sapiens 49-81 9705855-4 1998 In the cells, most all-trans retinol is bound to cellular retinol binding protein (CRBP). Vitamin A 29-36 retinol binding protein 1 Homo sapiens 83-87 9705855-5 1998 Whether retinoic acid is produced from the free or CRBP-bound retinol in vivo is not known. Vitamin A 62-69 retinol binding protein 1 Homo sapiens 51-55 9705855-6 1998 The current study investigated whether human medium-chain alcohol/retinol dehydrogenases (ADH) can oxidize the CRBP-bound retinol. Vitamin A 66-73 retinol binding protein 1 Homo sapiens 111-115 9705855-7 1998 The results of this study suggest that retinol bound to CRBP cannot be channeled to the active site of ADH. Vitamin A 39-46 retinol binding protein 1 Homo sapiens 56-60 9848156-3 1998 Retinol can specifically interacts with nuclei, nuclear envelope and chromatin only when it presents as a complex with CRBP. Vitamin A 0-7 retinol binding protein 1 Homo sapiens 119-123 9452451-1 1998 The hypothesis that the cellular uptake of retinol involves the specific interaction of a plasma membrane receptor with serum retinol-binding protein (RBP) at the extracellular surface followed by ligand transfer to cytoplasmic cellular retinol-binding protein (CRBP) has been investigated. Vitamin A 43-50 retinol binding protein 1 Homo sapiens 262-266 9452451-3 1998 [3H]Retinol transfer from RBP to CRBP-Strep was monitored by measuring the radioactivity associated with CRBP-Strep retained by an immobilized streptavidin resin. Vitamin A 4-11 retinol binding protein 1 Homo sapiens 33-37 9452451-3 1998 [3H]Retinol transfer from RBP to CRBP-Strep was monitored by measuring the radioactivity associated with CRBP-Strep retained by an immobilized streptavidin resin. Vitamin A 4-11 retinol binding protein 1 Homo sapiens 105-109 9452451-4 1998 Using this assay system, we have demonstrated that optimal retinol uptake is achieved with holo-RBP, the membrane receptor and apo-CRBP. Vitamin A 59-66 retinol binding protein 1 Homo sapiens 131-135 9452451-7 1998 Taken together, the data suggest that the RBP receptor, through specific interactions with the binding proteins, participates (either directly or via associated proteins) in the mechanism which mediates the transfer of retinol from extracellular RBP to intracellular CRBP. Vitamin A 219-226 retinol binding protein 1 Homo sapiens 267-271 9037178-1 1997 The cellular retinol binding proteins, CRBP and CRBP II, are implicated in the cellular uptake of retinol and intracellular trafficking of retinol between sites of metabolic processing. Vitamin A 13-20 retinol binding protein 1 Homo sapiens 39-43 9037178-1 1997 The cellular retinol binding proteins, CRBP and CRBP II, are implicated in the cellular uptake of retinol and intracellular trafficking of retinol between sites of metabolic processing. Vitamin A 98-105 retinol binding protein 1 Homo sapiens 39-43 9037178-1 1997 The cellular retinol binding proteins, CRBP and CRBP II, are implicated in the cellular uptake of retinol and intracellular trafficking of retinol between sites of metabolic processing. Vitamin A 98-105 retinol binding protein 1 Homo sapiens 39-43 9037178-2 1997 19F-NMR studies of retinol transfer between CRBP and CRBP II and phospholipid vesicles, using either fluorine-labeled ligand or protein, demonstrated that there was significantly more transfer of retinol from CRBP II to lipid vesicles than from CRBP. Vitamin A 19-26 retinol binding protein 1 Homo sapiens 44-48 9037178-2 1997 19F-NMR studies of retinol transfer between CRBP and CRBP II and phospholipid vesicles, using either fluorine-labeled ligand or protein, demonstrated that there was significantly more transfer of retinol from CRBP II to lipid vesicles than from CRBP. Vitamin A 19-26 retinol binding protein 1 Homo sapiens 53-57 9037178-2 1997 19F-NMR studies of retinol transfer between CRBP and CRBP II and phospholipid vesicles, using either fluorine-labeled ligand or protein, demonstrated that there was significantly more transfer of retinol from CRBP II to lipid vesicles than from CRBP. Vitamin A 196-203 retinol binding protein 1 Homo sapiens 44-48 9037178-2 1997 19F-NMR studies of retinol transfer between CRBP and CRBP II and phospholipid vesicles, using either fluorine-labeled ligand or protein, demonstrated that there was significantly more transfer of retinol from CRBP II to lipid vesicles than from CRBP. Vitamin A 196-203 retinol binding protein 1 Homo sapiens 53-57 7615982-1 1995 We examined the regulation of cellular retinol-binding protein (CRBP) mRNA and protein expression in human skin in vivo by all-trans retinoic acid and all-trans retinol. Vitamin A 39-46 retinol binding protein 1 Homo sapiens 64-68 7615982-1 1995 We examined the regulation of cellular retinol-binding protein (CRBP) mRNA and protein expression in human skin in vivo by all-trans retinoic acid and all-trans retinol. Vitamin A 161-168 retinol binding protein 1 Homo sapiens 64-68 7615982-2 1995 Treatment of human skin for 24 h with all-trans retinoic acid (0.1%) or all-trans retinol (1.6%) induced CRBP mRNA 5.5-fold (p < 0.01, n = 10) and 5.7-fold (p < 0.01, n = 5), respectively, compared with skin treated with vehicle or sodium lauryl sulfate (used as an irritant control). Vitamin A 82-89 retinol binding protein 1 Homo sapiens 105-109 7615982-3 1995 In vitro translation of poly A+ RNA from all-trans retinoic acid, all-trans retinol, sodium lauryl sulfate, and vehicle-treated human skin demonstrated that the observed increased CRBP mRNA in all-trans retinoic acid- and all-trans retinol-treated skin was able to direct increased (2.3-2.9-fold) CRBP protein synthesis. Vitamin A 76-83 retinol binding protein 1 Homo sapiens 180-184 7615982-3 1995 In vitro translation of poly A+ RNA from all-trans retinoic acid, all-trans retinol, sodium lauryl sulfate, and vehicle-treated human skin demonstrated that the observed increased CRBP mRNA in all-trans retinoic acid- and all-trans retinol-treated skin was able to direct increased (2.3-2.9-fold) CRBP protein synthesis. Vitamin A 232-239 retinol binding protein 1 Homo sapiens 180-184 7615982-6 1995 In addition, functional CRBP levels measured by [3H]all-trans retinol binding were elevated 1.9-fold (p < 0.01, n = 6) and 3.5-fold (p < 0.01, n = 6) at 24 and 94 h, respectively, after all-trans retinoic acid treatment, compared with vehicle- or sodium lauryl sulfate-treated skin. Vitamin A 62-69 retinol binding protein 1 Homo sapiens 24-28 7615982-9 1995 These data demonstrate that CRBP expression in human skin in vivo is regulated by exogenous all-trans retinoic acid and all-trans retinol. Vitamin A 130-137 retinol binding protein 1 Homo sapiens 28-32 7864119-3 1995 Compared with control cell lines, retinol uptake increased up to twofold by overexpression of CRBP II and up to 2.9-fold by coexpression of CRBP and CRBP II. Vitamin A 34-41 retinol binding protein 1 Homo sapiens 94-98 7864119-8 1995 Thus these studies provide evidence that intestinal retinol uptake, retinyl ester synthesis, and retinyl ester secretion are correlated with levels of CRBP and CRBP II and that the effects of CRBP on retinyl ester secretion can be distinguished from those of CRBP II. Vitamin A 52-59 retinol binding protein 1 Homo sapiens 151-155 7864119-8 1995 Thus these studies provide evidence that intestinal retinol uptake, retinyl ester synthesis, and retinyl ester secretion are correlated with levels of CRBP and CRBP II and that the effects of CRBP on retinyl ester secretion can be distinguished from those of CRBP II. Vitamin A 52-59 retinol binding protein 1 Homo sapiens 160-164 9788738-7 1998 Moreover, we showed that the cellular retinol binding protein (CRBP-I) expression in these cells, relevant for both uptake and esterification of retinol, responds to the extracellular retinol status, and is correlated to the retinol binding capacity of the cytosol. Vitamin A 38-45 retinol binding protein 1 Homo sapiens 63-69 9788738-7 1998 Moreover, we showed that the cellular retinol binding protein (CRBP-I) expression in these cells, relevant for both uptake and esterification of retinol, responds to the extracellular retinol status, and is correlated to the retinol binding capacity of the cytosol. Vitamin A 145-152 retinol binding protein 1 Homo sapiens 29-61 9788738-7 1998 Moreover, we showed that the cellular retinol binding protein (CRBP-I) expression in these cells, relevant for both uptake and esterification of retinol, responds to the extracellular retinol status, and is correlated to the retinol binding capacity of the cytosol. Vitamin A 145-152 retinol binding protein 1 Homo sapiens 63-69 9788738-7 1998 Moreover, we showed that the cellular retinol binding protein (CRBP-I) expression in these cells, relevant for both uptake and esterification of retinol, responds to the extracellular retinol status, and is correlated to the retinol binding capacity of the cytosol. Vitamin A 145-152 retinol binding protein 1 Homo sapiens 29-61 9788738-7 1998 Moreover, we showed that the cellular retinol binding protein (CRBP-I) expression in these cells, relevant for both uptake and esterification of retinol, responds to the extracellular retinol status, and is correlated to the retinol binding capacity of the cytosol. Vitamin A 145-152 retinol binding protein 1 Homo sapiens 63-69 8841765-3 1996 The retinol-binding proteins CRBP I and CRBP II appear to play an essential role in retinyl ester hydrolysis and formation and in retinoic acid formation. Vitamin A 4-11 retinol binding protein 1 Homo sapiens 29-35 7669770-4 1995 The greater affinity of CRBP for all-trans-retinol has been attributed to the presence of an amino-aromatic hydrogen bond, which is absent in CRBP(II). Vitamin A 33-50 retinol binding protein 1 Homo sapiens 24-28 7669770-6 1995 Spectral analyses of retinol when bound to the wild-type and mutant CRBP suggested that it adopted an identical conformation within both proteins, a conformation that was distinct from that of retinol bound to CRBP(II), both wild-type and mutant. Vitamin A 21-28 retinol binding protein 1 Homo sapiens 68-72 7657389-3 1995 We have thus investigated the expression of nuclear retinoic acid receptors (RARs and RXRs) and cellular binding proteins for retinol (CRBP) and retinoic acid (CRABP) in endometrial adenocarcinoma of the endometrioid histological subtype. Vitamin A 126-133 retinol binding protein 1 Homo sapiens 135-139 7835405-1 1994 During a bleach, all-trans-retinol passes from the photoreceptor outer segments to the retinal pigment epithelium (RPE), where retinol is found associated with cellular retinol-binding protein (CRBP). Vitamin A 17-34 retinol binding protein 1 Homo sapiens 160-192 7835405-1 1994 During a bleach, all-trans-retinol passes from the photoreceptor outer segments to the retinal pigment epithelium (RPE), where retinol is found associated with cellular retinol-binding protein (CRBP). Vitamin A 17-34 retinol binding protein 1 Homo sapiens 194-198 7835405-1 1994 During a bleach, all-trans-retinol passes from the photoreceptor outer segments to the retinal pigment epithelium (RPE), where retinol is found associated with cellular retinol-binding protein (CRBP). Vitamin A 27-34 retinol binding protein 1 Homo sapiens 160-192 7835405-1 1994 During a bleach, all-trans-retinol passes from the photoreceptor outer segments to the retinal pigment epithelium (RPE), where retinol is found associated with cellular retinol-binding protein (CRBP). Vitamin A 27-34 retinol binding protein 1 Homo sapiens 194-198 7835405-2 1994 Interphotoreceptor retinoid-binding protein (IRBP) is thought to facilitate this exchange, but the transfer of retinol between IRBP and CRBP has not been explored. Vitamin A 111-118 retinol binding protein 1 Homo sapiens 136-140 7835405-3 1994 In this study we used a mixture of purified IRBP and CRBP as a model system to measure the amount and rate of retinol transfer between the two proteins. Vitamin A 110-117 retinol binding protein 1 Homo sapiens 53-57 7835405-4 1994 When retinol is transferred from IRBP to CRBP, its absorbance maximum shifts from 330 to 350 nm. Vitamin A 5-12 retinol binding protein 1 Homo sapiens 41-45 7835405-5 1994 By monitoring the increase in absorbance at 350 nm after mixing CRBP with IRBP-bound retinol, we measured the amount and time course of retinol transfer from IRBP to CRBP. Vitamin A 85-92 retinol binding protein 1 Homo sapiens 166-170 7835405-5 1994 By monitoring the increase in absorbance at 350 nm after mixing CRBP with IRBP-bound retinol, we measured the amount and time course of retinol transfer from IRBP to CRBP. Vitamin A 136-143 retinol binding protein 1 Homo sapiens 64-68 7835405-5 1994 By monitoring the increase in absorbance at 350 nm after mixing CRBP with IRBP-bound retinol, we measured the amount and time course of retinol transfer from IRBP to CRBP. Vitamin A 136-143 retinol binding protein 1 Homo sapiens 166-170 7835405-7 1994 As determined by measuring the change in absorbance at 350 nm, the mean percentage of IRBP-bound retinol transferred to CRBP was 103 +/- 11% (n = 9). Vitamin A 97-104 retinol binding protein 1 Homo sapiens 120-124 7835405-9 1994 IRBP that was separated from the mixture by HPLC contained little or no retinol, while the isolated CRBP was nearly saturated with retinol. Vitamin A 131-138 retinol binding protein 1 Homo sapiens 100-104 7835405-11 1994 The distribution of retinol between these two proteins was consistent with the nearly 100-fold higher affinity of CRBP for retinol compared with IRBP. Vitamin A 20-27 retinol binding protein 1 Homo sapiens 114-118 7835405-11 1994 The distribution of retinol between these two proteins was consistent with the nearly 100-fold higher affinity of CRBP for retinol compared with IRBP. Vitamin A 123-130 retinol binding protein 1 Homo sapiens 114-118 7683727-7 1993 In CRBP, the all-trans-retinol has a planar conformation with its alcohol group hydrogen bonding to the side-chain of glutamine 108 (equivalent to residue 106 in P2). Vitamin A 13-30 retinol binding protein 1 Homo sapiens 3-7 7683727-8 1993 The local interactions of glutamine 108 explain CRBP"s preference for binding retinol rather than retinal. Vitamin A 78-85 retinol binding protein 1 Homo sapiens 48-52 8387121-3 1993 CRBP (I) functions in cellular uptake of retinol from the plasma, solubilizes and renders retinol nontoxic in the aqueous system, and presents retinol to the appropriate enzymes to biosynthesize retinoic acid (an active form of retinoids) or retinyl esters (a storage form). Vitamin A 41-48 retinol binding protein 1 Homo sapiens 0-4 8463337-1 1993 The mammalian small intestine contains two related cellular retinol-binding proteins, CRBP and CRBP II, which are thought to have distinct functions. Vitamin A 60-67 retinol binding protein 1 Homo sapiens 86-90 8463337-5 1993 Retinol uptake and retinyl ester synthesis were increased up to 2-fold by coexpression of CRBP or over-expression of CRBP II. Vitamin A 0-7 retinol binding protein 1 Homo sapiens 90-94 8463337-11 1993 These studies indicate that CRBP and CRBP II levels are determinants of intracellular retinol accumulation and esterification, and they suggest that CRBP-bound retinol or a metabolite can regulate the expression of CRBP II in the mammalian intestine. Vitamin A 86-93 retinol binding protein 1 Homo sapiens 28-32 8463337-11 1993 These studies indicate that CRBP and CRBP II levels are determinants of intracellular retinol accumulation and esterification, and they suggest that CRBP-bound retinol or a metabolite can regulate the expression of CRBP II in the mammalian intestine. Vitamin A 86-93 retinol binding protein 1 Homo sapiens 37-41 8463337-11 1993 These studies indicate that CRBP and CRBP II levels are determinants of intracellular retinol accumulation and esterification, and they suggest that CRBP-bound retinol or a metabolite can regulate the expression of CRBP II in the mammalian intestine. Vitamin A 160-167 retinol binding protein 1 Homo sapiens 28-32 8387121-3 1993 CRBP (I) functions in cellular uptake of retinol from the plasma, solubilizes and renders retinol nontoxic in the aqueous system, and presents retinol to the appropriate enzymes to biosynthesize retinoic acid (an active form of retinoids) or retinyl esters (a storage form). Vitamin A 90-97 retinol binding protein 1 Homo sapiens 0-4 8387121-3 1993 CRBP (I) functions in cellular uptake of retinol from the plasma, solubilizes and renders retinol nontoxic in the aqueous system, and presents retinol to the appropriate enzymes to biosynthesize retinoic acid (an active form of retinoids) or retinyl esters (a storage form). Vitamin A 90-97 retinol binding protein 1 Homo sapiens 0-4 8387121-5 1993 CRBP (III) is recognized as one of oncofetal proteins and binds both retinol and retinoic acid, the function of which still remains unclear. Vitamin A 69-76 retinol binding protein 1 Homo sapiens 0-4 8385449-5 1993 In this article I propose that the amount of RA reaching the nucleus in different embryonic tissues is modulated by a mechanism involving three cytoplasmic binding proteins for retinol (CRBP I) and retinoic acid (CRABP I and II). Vitamin A 177-184 retinol binding protein 1 Homo sapiens 186-192 8440409-7 1993 For example, the cellular retinol-binding protein, CRBP, has been implicated in retinol uptake, retinol esterification, mobilization of retinyl esters, and the initial oxidation of retinol to retinaldehyde. Vitamin A 26-33 retinol binding protein 1 Homo sapiens 51-55 8440409-7 1993 For example, the cellular retinol-binding protein, CRBP, has been implicated in retinol uptake, retinol esterification, mobilization of retinyl esters, and the initial oxidation of retinol to retinaldehyde. Vitamin A 80-87 retinol binding protein 1 Homo sapiens 51-55 8440409-7 1993 For example, the cellular retinol-binding protein, CRBP, has been implicated in retinol uptake, retinol esterification, mobilization of retinyl esters, and the initial oxidation of retinol to retinaldehyde. Vitamin A 80-87 retinol binding protein 1 Homo sapiens 51-55 8144697-7 1993 Within the cell, retinol and its derivatives are solubilized by intracellular retinoid binding proteins that are selective for retinol (cellular retinol binding protein, CRBP) and 11-cis retinoids (cellular retinal binding protein, CRALBP). Vitamin A 17-24 retinol binding protein 1 Homo sapiens 170-174 8144697-7 1993 Within the cell, retinol and its derivatives are solubilized by intracellular retinoid binding proteins that are selective for retinol (cellular retinol binding protein, CRBP) and 11-cis retinoids (cellular retinal binding protein, CRALBP). Vitamin A 127-134 retinol binding protein 1 Homo sapiens 170-174 1562584-1 1992 Holo-CRBP (cellular retinol binding protein) is recognized specifically by an NADP-dependent microsomal retinol dehydrogenase and protects retinol from conversion into retinal by NAD and NADPH dependent dehydrogenases. Vitamin A 104-111 retinol binding protein 1 Homo sapiens 5-9 1562584-1 1992 Holo-CRBP (cellular retinol binding protein) is recognized specifically by an NADP-dependent microsomal retinol dehydrogenase and protects retinol from conversion into retinal by NAD and NADPH dependent dehydrogenases. Vitamin A 20-27 retinol binding protein 1 Homo sapiens 5-9 1322170-2 1992 Retinol in cells is bound by either cellular retinol binding protein (CRBP), present in most tissues including liver, or cellular retinol binding protein type II [CRBP(II)], present in the absorptive cell of the small intestine. Vitamin A 0-7 retinol binding protein 1 Homo sapiens 36-68 1322170-2 1992 Retinol in cells is bound by either cellular retinol binding protein (CRBP), present in most tissues including liver, or cellular retinol binding protein type II [CRBP(II)], present in the absorptive cell of the small intestine. Vitamin A 0-7 retinol binding protein 1 Homo sapiens 70-74 1322170-4 1992 Esterification of free retinol by both liver and intestinal LRAT resulted in Km values (0.63 and 0.44 microM, respectively) similar to those obtained for esterification of retinol-CRBP (0.20 and 0.78 microM, respectively) and esterification of retinol-CRBP(II) (0.24 and 0.32 microM, respectively). Vitamin A 23-30 retinol binding protein 1 Homo sapiens 180-184 1322170-4 1992 Esterification of free retinol by both liver and intestinal LRAT resulted in Km values (0.63 and 0.44 microM, respectively) similar to those obtained for esterification of retinol-CRBP (0.20 and 0.78 microM, respectively) and esterification of retinol-CRBP(II) (0.24 and 0.32 microM, respectively). Vitamin A 172-179 retinol binding protein 1 Homo sapiens 180-184 1322170-4 1992 Esterification of free retinol by both liver and intestinal LRAT resulted in Km values (0.63 and 0.44 microM, respectively) similar to those obtained for esterification of retinol-CRBP (0.20 and 0.78 microM, respectively) and esterification of retinol-CRBP(II) (0.24 and 0.32 microM, respectively). Vitamin A 172-179 retinol binding protein 1 Homo sapiens 180-184 1322170-6 1992 Evidence for such interaction was obtained when apo-CRBP proved to be a potent competitive inhibitor of LRAT, with a KI (0.21 microM) lower than the Km for CRBP-retinol (0.78 microM). Vitamin A 161-168 retinol binding protein 1 Homo sapiens 156-160 1322170-8 1992 Apo-CRBP reacted with 4 mM p-(chloromercuri)benzenesulfonic acid lost retinol binding ability but retained the ability to inhibit LRAT, confirming that the inhibition could not be explained by a reduction in the concentration of free retinol. Vitamin A 70-77 retinol binding protein 1 Homo sapiens 4-8 1322170-8 1992 Apo-CRBP reacted with 4 mM p-(chloromercuri)benzenesulfonic acid lost retinol binding ability but retained the ability to inhibit LRAT, confirming that the inhibition could not be explained by a reduction in the concentration of free retinol. Vitamin A 234-241 retinol binding protein 1 Homo sapiens 4-8 1542003-1 1992 Two forms of human cellular retinol-binding protein, type II [hCRBP(II)A and hCRBP(II)B], were purified from the small intestine in a three-step purification procedure. Vitamin A 28-35 retinol binding protein 1 Homo sapiens 62-67 1542003-1 1992 Two forms of human cellular retinol-binding protein, type II [hCRBP(II)A and hCRBP(II)B], were purified from the small intestine in a three-step purification procedure. Vitamin A 28-35 retinol binding protein 1 Homo sapiens 77-82 1542003-5 1992 The ability of pure hCRBP(II) to bind all-trans-retinol, retinal and retinoic acid was examined by competitive binding assay and compared with the binding specificity of pure human cellular retinol-binding protein (hCRBP). Vitamin A 38-55 retinol binding protein 1 Homo sapiens 20-25 1542003-7 1992 Retinal competed with retinol for binding to hCRBP(II) but not to hCRBP, consistent with what was observed for the homologous proteins of rats. Vitamin A 22-29 retinol binding protein 1 Homo sapiens 45-50 1768424-10 1991 Since CD-270(OH) binds to the CRBP this may lead to the development of new synthetic analogues of ROL which could be used as tools for the study of the role of CRBP in the transport and metabolism of ROL. Vitamin A 98-101 retinol binding protein 1 Homo sapiens 30-34 1768424-2 1991 Tritiated analogues of retinol (ROL) and retinoic acid corresponding to substituted benzo[b]thiophene (CD-270) alcohol and carboxylic acid, respectively, were used for the binding studies of the cellular retinoic acid-(CRABP-) and retinol-(CRBP-) binding proteins in human epidermal cells and serum retinol-binding protein (RBP). Vitamin A 23-30 retinol binding protein 1 Homo sapiens 240-244 1768424-10 1991 Since CD-270(OH) binds to the CRBP this may lead to the development of new synthetic analogues of ROL which could be used as tools for the study of the role of CRBP in the transport and metabolism of ROL. Vitamin A 200-203 retinol binding protein 1 Homo sapiens 160-164 2145932-5 1990 However, patients with stage IV disease had higher C-RBP levels than patients at stages II and III (P less than 0.0001), which suggested altered intracellular mobilization of retinol in the tumour, probably as an indirect consequence of inadequate nutrient intake. Vitamin A 175-182 retinol binding protein 1 Homo sapiens 51-56 2546159-3 1989 Further, the induction of alkaline phosphatase by retinol in these cells was blocked by treatment with 30 microM antisense oligo(dN) to cRBP or hnRAR but not by 30 microM of sense oligo(dN) to cRBP. Vitamin A 50-57 retinol binding protein 1 Homo sapiens 136-140 34775955-1 2021 BACKGROUND: CRBP-1, a cytosolic chaperone of vitamin A, is identified in a serious number of cancers; however, its biological role in hepatocellular carcinoma (HCC) needs to be further explored. Vitamin A 45-54 retinol binding protein 1 Homo sapiens 12-18 34480899-10 2021 These results suggest that P450 27C1 directly accepts all-trans retinol and retinaldehyde from CRBP-1 and all-trans retinoic acid from CRABP-2, but not from CRABP-1. Vitamin A 64-71 retinol binding protein 1 Homo sapiens 95-101 2611253-7 1989 LRAT utilized an endogenous acyl donor and either unbound retinol or retinol complexed with cellular retinol-binding protein (CRBP) to catalyze the synthesis of retinyl linoleate, retinyl oleate, retinyl palmitate, and retinyl stearate. Vitamin A 69-76 retinol binding protein 1 Homo sapiens 92-124 2611253-7 1989 LRAT utilized an endogenous acyl donor and either unbound retinol or retinol complexed with cellular retinol-binding protein (CRBP) to catalyze the synthesis of retinyl linoleate, retinyl oleate, retinyl palmitate, and retinyl stearate. Vitamin A 69-76 retinol binding protein 1 Homo sapiens 126-130 2546159-3 1989 Further, the induction of alkaline phosphatase by retinol in these cells was blocked by treatment with 30 microM antisense oligo(dN) to cRBP or hnRAR but not by 30 microM of sense oligo(dN) to cRBP. Vitamin A 50-57 retinol binding protein 1 Homo sapiens 193-197 2920176-3 1989 There was a statistically significant correlation between the retinol and the CRBP concentrations in the same tumour (P less than 0.001; r = 0.622). Vitamin A 62-69 retinol binding protein 1 Homo sapiens 78-82 2920176-4 1989 Calculation of the maximal extent of retinol-saturation of CRBP showed low values (range: 9-26%). Vitamin A 37-44 retinol binding protein 1 Homo sapiens 59-63 2920176-11 1989 The cellular retinol concentration, on the other hand, appears proportional to CRBP content. Vitamin A 13-20 retinol binding protein 1 Homo sapiens 79-83 2645590-4 1989 Human skin extracts incubated with either [3H]retinol or [3H]retinoic acid and analyzed by PAGE is a novel technique for the study of cellular retinol-(CRBP) and retinoic acid-(CRABP) binding proteins; it allows one to more specifically analyse these binding proteins and differentiate them from RBP. Vitamin A 46-53 retinol binding protein 1 Homo sapiens 152-156 2645590-4 1989 Human skin extracts incubated with either [3H]retinol or [3H]retinoic acid and analyzed by PAGE is a novel technique for the study of cellular retinol-(CRBP) and retinoic acid-(CRABP) binding proteins; it allows one to more specifically analyse these binding proteins and differentiate them from RBP. Vitamin A 143-150 retinol binding protein 1 Homo sapiens 152-156 3579304-1 1987 We have reported previously that cellular retinol-binding protein (CRBP) is able to transfer retinol to specific binding sites in nuclei and chromatin. Vitamin A 42-49 retinol binding protein 1 Homo sapiens 67-71 3178828-1 1988 This report demonstrates that exogenous phosphatidylcholine will serve as an acyl donor for the esterification of retinol complexed to cellular retinol-binding protein (CRBP) by human and rat liver microsomal preparations. Vitamin A 114-121 retinol binding protein 1 Homo sapiens 135-167 3178828-1 1988 This report demonstrates that exogenous phosphatidylcholine will serve as an acyl donor for the esterification of retinol complexed to cellular retinol-binding protein (CRBP) by human and rat liver microsomal preparations. Vitamin A 114-121 retinol binding protein 1 Homo sapiens 169-173 2457503-2 1988 In the present study the levels of cellular retinoic acid (CRABP)- and retinol (CRBP)-binding proteins were measured in cultured normal human epidermal keratinocytes. Vitamin A 71-78 retinol binding protein 1 Homo sapiens 80-84 3281946-2 1988 Retinol bound to cellular retinol-binding protein (CRBP) was available for esterification by liver microsomes in the absence of exogenous acyl donors. Vitamin A 0-7 retinol binding protein 1 Homo sapiens 51-55 2825608-8 1987 In contrast, CRBP (II) appears to be involved in the intestinal absorption of vitamin A and, in particular, may direct retinol to a specific esterifying enzyme, resulting in the production of fatty acyl esters of retinol that are incorporated into chylomicrons for release to the lymph. Vitamin A 78-87 retinol binding protein 1 Homo sapiens 13-17 2825608-8 1987 In contrast, CRBP (II) appears to be involved in the intestinal absorption of vitamin A and, in particular, may direct retinol to a specific esterifying enzyme, resulting in the production of fatty acyl esters of retinol that are incorporated into chylomicrons for release to the lymph. Vitamin A 119-126 retinol binding protein 1 Homo sapiens 13-17 2825608-8 1987 In contrast, CRBP (II) appears to be involved in the intestinal absorption of vitamin A and, in particular, may direct retinol to a specific esterifying enzyme, resulting in the production of fatty acyl esters of retinol that are incorporated into chylomicrons for release to the lymph. Vitamin A 213-220 retinol binding protein 1 Homo sapiens 13-17 2839288-1 1988 Binding proteins for retinoic acid (cellular retinoid acid binding protein, CRABP), and for vitamin A (cellular retinol binding protein, CRBP) have been demonstrated in various cell types; these binding proteins display the characteristics of receptors. Vitamin A 92-101 retinol binding protein 1 Homo sapiens 137-141 2825608-7 1987 Cellular retinol-binding protein appears to have several roles, including (1) delivering retinol to specific binding sites within the nucleus and (2) participating in the transepithelial movement of retinol across certain blood-organ barriers. Vitamin A 89-96 retinol binding protein 1 Homo sapiens 0-32 3611082-1 1987 Cellular retinol-binding protein (CRBP) and cellular retinol-binding protein, type ii (CRBP(II] are cytoplasmic proteins that bind trans-retinol as an endogenous ligand. Vitamin A 131-144 retinol binding protein 1 Homo sapiens 0-32 3611082-1 1987 Cellular retinol-binding protein (CRBP) and cellular retinol-binding protein, type ii (CRBP(II] are cytoplasmic proteins that bind trans-retinol as an endogenous ligand. Vitamin A 131-144 retinol binding protein 1 Homo sapiens 34-38 3611082-3 1987 Employing fluorescence, absorbance, and competition studies, the ability of pure preparations of CRBP(II) and CRBP to bind various members of the vitamin A family has been examined. Vitamin A 146-155 retinol binding protein 1 Homo sapiens 97-101 3611082-5 1987 CRBP bound those retinol isomers with similar affinities, but did not bind trans-retinaldehyde. Vitamin A 17-24 retinol binding protein 1 Homo sapiens 0-4 3611082-9 1987 It appears that CRBP(II) and CRBP bind trans-retinol, 13-cis-retinol, and 3-dehydroretinol in a planar configuration. Vitamin A 39-52 retinol binding protein 1 Homo sapiens 16-20 2833143-2 1987 Studies on CRBP and CRABP suggest that both retinol and retinoic acid are involved in maintaining testicular function. Vitamin A 44-51 retinol binding protein 1 Homo sapiens 11-15 3025302-1 1987 Since cellular retinol- and retinoic acid-binding proteins (CRBP and CRABP) mediate the effects of vitamin A on epidermal differentiation, the levels of these binding proteins were measured in the epidermal and dermal layers of newborn, human foreskin as well as in primary cultures of keratinocytes and fibroblasts from these layers. Vitamin A 99-108 retinol binding protein 1 Homo sapiens 60-64 3579304-5 1987 On the other hand, cellular retinol-binding protein type II (CRBP(II], whose amino acid sequence shows a considerable similarity to CRBP, did compete for the transfer of retinol from the R-CRBP complex, but less effectively than CRBP. Vitamin A 28-35 retinol binding protein 1 Homo sapiens 61-65 3579304-5 1987 On the other hand, cellular retinol-binding protein type II (CRBP(II], whose amino acid sequence shows a considerable similarity to CRBP, did compete for the transfer of retinol from the R-CRBP complex, but less effectively than CRBP. Vitamin A 28-35 retinol binding protein 1 Homo sapiens 132-136 3579304-5 1987 On the other hand, cellular retinol-binding protein type II (CRBP(II], whose amino acid sequence shows a considerable similarity to CRBP, did compete for the transfer of retinol from the R-CRBP complex, but less effectively than CRBP. Vitamin A 28-35 retinol binding protein 1 Homo sapiens 132-136 2992894-6 1985 Retinol and RA might be translocated to nuclei by their respective binding proteins [cellular retinol-binding protein (CRBP) and cellular retinoic acid-binding protein (CRABP)]: isolated EC nuclei have specific, independent binding sites for both holoproteins but not their ligands. Vitamin A 0-7 retinol binding protein 1 Homo sapiens 85-117 3015289-2 1986 Intracellular metabolism is complex and involves the binding to specific receptors for retinol (CRBP) and retinoic acid (CRABP) followed by a nuclear translocation. Vitamin A 87-94 retinol binding protein 1 Homo sapiens 96-100 2995045-1 1985 The cellular content of receptors for retinol (CRBP) and retinoic acid (CRABP) was measured in 148 human mammary carcinomas. Vitamin A 38-45 retinol binding protein 1 Homo sapiens 47-51 3751963-5 1986 The wide distribution of CRBP in human organs stresses the importance of this protein in the metabolism of vitamin A throughout the body. Vitamin A 107-116 retinol binding protein 1 Homo sapiens 25-29 2425004-1 1986 Cellular retinol (CRBP)-and retinoic acid (CRABP)-binding proteins were determined in samples of lesional and nonlesional skin of psoriatic patients, before and during oral administration of a synthetic retinoid, Etretin (Ro 10-1670). Vitamin A 9-16 retinol binding protein 1 Homo sapiens 18-22 2992894-6 1985 Retinol and RA might be translocated to nuclei by their respective binding proteins [cellular retinol-binding protein (CRBP) and cellular retinoic acid-binding protein (CRABP)]: isolated EC nuclei have specific, independent binding sites for both holoproteins but not their ligands. Vitamin A 0-7 retinol binding protein 1 Homo sapiens 119-123 2992894-8 1985 Interestingly, incubation with retinol promotes movement of both CRBP and CRABP into the nucleoplasmic fraction. Vitamin A 31-38 retinol binding protein 1 Homo sapiens 65-69 6279709-4 1982 A number of tissues of rats, humans, and other species contain soluble binding proteins with specificity for either retinol (cellular retinol-binding protein, CRBP) or retinoic acid (cellular retinoic acid-binding protein, CRABP). Vitamin A 116-123 retinol binding protein 1 Homo sapiens 159-163 6684744-3 1983 Whereas retinol-binding (CRBP = cellular retinol-binding protein) could only be detected in a few cases, binding for retinoic acid (CRABP = cellular retinoic acid-binding protein) was present in all specimens investigated. Vitamin A 8-15 retinol binding protein 1 Homo sapiens 25-29 6684744-6 1983 CRBP and CRABP are assumed to be mediating factors for the retinol and retinoic acid action. Vitamin A 59-66 retinol binding protein 1 Homo sapiens 0-4 7199377-5 1982 Many of the properties of human CRBP including molecular weight, amino acid composition, and spectrum of bound retinol are similar to those observed previously for rat CRBP. Vitamin A 111-118 retinol binding protein 1 Homo sapiens 32-36 7199377-6 1982 The availability of pure human CRBP should aid in elucidating its role in the action of retinol and also is more easily monitoring the considerable changes in level of this protein reported in some human cancers. Vitamin A 88-95 retinol binding protein 1 Homo sapiens 31-35 6685481-5 1983 Thus, this method, which resolves CRABP and CRBP in crude mixtures from the majority of cytosolic proteins, should be a valuable tool in the evaluation of vitamin A-binding protein interactions and their biological significance. Vitamin A 155-164 retinol binding protein 1 Homo sapiens 44-48 7193082-1 1980 Human colon adenocarcinomas and adjacent non-cancerous, normal colon from the same patient were assayed for the presence and amounts of cellular binding proteins for retinol (CRBP) and retinoic acid (CRABP) by sucrose gradient analysis. Vitamin A 166-173 retinol binding protein 1 Homo sapiens 175-179 6942700-8 1981 The retinol so delivered enters the target cell, where it may become associated with the intracellular binding protein for retinol (CRBP). Vitamin A 4-11 retinol binding protein 1 Homo sapiens 132-136 6942700-8 1981 The retinol so delivered enters the target cell, where it may become associated with the intracellular binding protein for retinol (CRBP). Vitamin A 123-130 retinol binding protein 1 Homo sapiens 132-136 6942700-9 1981 A number of tissues of rats, humans, and other species contain soluble proteins with binding specificity for retinol (CRBP) or for retinoic acid (CRABP). Vitamin A 109-116 retinol binding protein 1 Homo sapiens 118-122 30613363-3 2018 Cellular retinol binding protein (CRBPI) regulates intracellular vitamin A bioavailability and contributes to maintain skin homeostasis. Vitamin A 65-74 retinol binding protein 1 Homo sapiens 0-32 30613363-3 2018 Cellular retinol binding protein (CRBPI) regulates intracellular vitamin A bioavailability and contributes to maintain skin homeostasis. Vitamin A 65-74 retinol binding protein 1 Homo sapiens 34-39 27830500-7 2016 Metabolic and functional defects manifested in knockouts of CRBP1, CRBP2 and CRBP3, however, illustrate their essentiality to health, and in the case of CRBP2, to survival during limited dietary vitamin A. Vitamin A 195-204 retinol binding protein 1 Homo sapiens 60-65 29567208-0 2018 Deciphering protein dynamics changes along the pathway of retinol uptake by cellular retinol-binding proteins 1 and 2. Vitamin A 58-65 retinol binding protein 1 Homo sapiens 85-117 29567208-2 2018 CRBP 1 and 2, sharing a 56% amino acid sequence identity, exhibit the highest binding affinities for retinol. Vitamin A 101-108 retinol binding protein 1 Homo sapiens 0-12 29567208-4 2018 Herein, the results of molecular dynamics simulations for the uptake of retinol by CRBP 1 and 2 are consistent with the presence of two different retinol entry points, both involving the "cap region" (alpha-helices I and II and neighboring loops). Vitamin A 72-79 retinol binding protein 1 Homo sapiens 83-95 29567208-4 2018 Herein, the results of molecular dynamics simulations for the uptake of retinol by CRBP 1 and 2 are consistent with the presence of two different retinol entry points, both involving the "cap region" (alpha-helices I and II and neighboring loops). Vitamin A 146-153 retinol binding protein 1 Homo sapiens 83-95 29567208-5 2018 We observed that a hydrophobic patch at the surface of the "portal region" (alpha-helix II, CD and EF loops) of CRBP 1 attracts retinol, which accesses the binding cavity through an opening generated by the concerted movements of Arg58 and Phe57, present in the CD loop. Vitamin A 128-135 retinol binding protein 1 Homo sapiens 112-118 29589070-5 2018 In addition, PSCs have a remarkable capacity for vitamin A uptake most likely through cellular retinol binding protein (CRBP). Vitamin A 49-58 retinol binding protein 1 Homo sapiens 86-118 29589070-5 2018 In addition, PSCs have a remarkable capacity for vitamin A uptake most likely through cellular retinol binding protein (CRBP). Vitamin A 49-58 retinol binding protein 1 Homo sapiens 120-124 28057518-0 2017 Structural and molecular determinants affecting the interaction of retinol with human CRBP1. Vitamin A 67-74 retinol binding protein 1 Homo sapiens 86-91 28057518-4 2017 Fluorometric titrations of wild type and mutant forms of apo-CRBP1, coupled with X-ray analyses, provided insight into structural and molecular determinants for the interaction of retinol with CRBP1. Vitamin A 180-187 retinol binding protein 1 Homo sapiens 61-66 28057518-4 2017 Fluorometric titrations of wild type and mutant forms of apo-CRBP1, coupled with X-ray analyses, provided insight into structural and molecular determinants for the interaction of retinol with CRBP1. Vitamin A 180-187 retinol binding protein 1 Homo sapiens 193-198 28057518-5 2017 An approximately stoichiometric binding of retinol to wild type apo-CRBP1 (Kd~4.5nM), significantly lower binding affinity for both mutants Q108L (Kd~65nM) and K40L (Kd~70nM) and very low binding affinity for the double mutant Q108L/K40L (Kd~250nM) were determined, respectively. Vitamin A 43-50 retinol binding protein 1 Homo sapiens 68-73 28057518-6 2017 Overall, our data indicate that the extensive apolar interactions between the ligand and hydrophobic residues lining the retinol binding cavity are sufficient to keep it in its position bound to CRBP1. Vitamin A 121-128 retinol binding protein 1 Homo sapiens 195-200 26900151-1 2016 Important in regulating the uptake, storage, and metabolism of retinoids, cellular retinol-binding protein 1 (CRBP1) is essential for trafficking vitamin A through the cytoplasm. Vitamin A 146-155 retinol binding protein 1 Homo sapiens 74-108 26900151-1 2016 Important in regulating the uptake, storage, and metabolism of retinoids, cellular retinol-binding protein 1 (CRBP1) is essential for trafficking vitamin A through the cytoplasm. Vitamin A 146-155 retinol binding protein 1 Homo sapiens 110-115 26900151-3 2016 Here we report the first structure of CRBP1 in a ligand-free form as well as ultra-high resolution structures of this protein bound to either all-trans-retinol or retinylamine, the latter a therapeutic retinoid that prevents light-induced retinal degeneration. Vitamin A 142-159 retinol binding protein 1 Homo sapiens 38-43 26880640-2 2016 The intracellular transport of vitamin A is assisted by proteins called cellular retinol-binding proteins (CRBP I/II). Vitamin A 31-40 retinol binding protein 1 Homo sapiens 107-111 26880640-7 2016 We propose in this report that LRAT physically interacts with CRBP and the LRAT-CRBP complex represents the binding pockets for both an acyl group and retinol, thus assuring the substrate specificity of LRAT. Vitamin A 151-158 retinol binding protein 1 Homo sapiens 62-66 26880640-7 2016 We propose in this report that LRAT physically interacts with CRBP and the LRAT-CRBP complex represents the binding pockets for both an acyl group and retinol, thus assuring the substrate specificity of LRAT. Vitamin A 151-158 retinol binding protein 1 Homo sapiens 80-84 27830500-4 2016 The major intracellular retinol-binding protein, CRBP1, likely enhances efficient retinoid use by providing a sink to facilitate retinol uptake from sRBP through the plasma membrane or via Stra6, delivering retinol or retinal to select enzymes that generate retinyl esters or retinoic acid, and protecting retinol/retinal from excess catabolism or opportunistic metabolism. Vitamin A 24-31 retinol binding protein 1 Homo sapiens 49-54 29720147-2 2018 Cellular retinol binding protein-1 (CRBP-1) as a carrier protein transports retinol from the liver storage site to peripheral tissue. Vitamin A 9-16 retinol binding protein 1 Homo sapiens 36-42 28592664-11 2017 However, expression of CRBP1, an intracellular carrier protein for retinol, increased, as did RBP4, a carrier protein for retinol in the blood, which can function in a paracrine manner. Vitamin A 67-74 retinol binding protein 1 Homo sapiens 23-28 28592664-11 2017 However, expression of CRBP1, an intracellular carrier protein for retinol, increased, as did RBP4, a carrier protein for retinol in the blood, which can function in a paracrine manner. Vitamin A 122-129 retinol binding protein 1 Homo sapiens 23-28 23890161-8 2014 CONCLUSION: This study provides evidence that functional HSCs coexpressing both LRAT and CRBP-1 that continue to maintain the ability to store vitamin A contribute in part to the development of portal fibrogenesis in addition to parenchymal fibrogenesis in patients with viral hepatitis. Vitamin A 143-152 retinol binding protein 1 Homo sapiens 89-95 25481334-3 2015 STRA6-mediated retinol transport and cell signaling are critically inter-dependent, and they both require the presence of cellular retinol-binding protein 1 (CRBP1), an intracellular retinol acceptor, as well as a retinol-metabolizing enzyme such as lecithin:retinol acyltransferase (LRAT). Vitamin A 15-22 retinol binding protein 1 Homo sapiens 122-156 25481334-3 2015 STRA6-mediated retinol transport and cell signaling are critically inter-dependent, and they both require the presence of cellular retinol-binding protein 1 (CRBP1), an intracellular retinol acceptor, as well as a retinol-metabolizing enzyme such as lecithin:retinol acyltransferase (LRAT). Vitamin A 15-22 retinol binding protein 1 Homo sapiens 158-163 25262571-3 2015 Vitamin A and its derivatives, collectively called retinoids, are solubilized by intracellular retinoid-binding proteins such as cellular retinol-binding protein (CRBP), cellular retinoic acid-binding protein (CRABP) and cellular retinal-binding protein (CRALBP). Vitamin A 0-9 retinol binding protein 1 Homo sapiens 129-161 25262571-3 2015 Vitamin A and its derivatives, collectively called retinoids, are solubilized by intracellular retinoid-binding proteins such as cellular retinol-binding protein (CRBP), cellular retinoic acid-binding protein (CRABP) and cellular retinal-binding protein (CRALBP). Vitamin A 0-9 retinol binding protein 1 Homo sapiens 163-167 24982334-6 2014 A2780 CRBP-1-transfected cells showed increased retinol-induced apoptosis, retinoid-induced reduced clonogenicity and down-regulation of proliferation and transcription genes, including AKT1, AKT3, EGFR, FOS, JUN, STAT1 and STAT5A. Vitamin A 48-55 retinol binding protein 1 Homo sapiens 6-12 26807202-2 2015 Cellular retinol binding protein-1 (CRBP-1) regulates retinol bioavailability and cell differentiation, but its role in lung cancerogenesis remains uncertain. Vitamin A 9-16 retinol binding protein 1 Homo sapiens 36-42 26807202-9 2015 At >1muM concentrations, all trans-retinoic acid and retinol reduced viability more in CRBP-1(+) than in CRBP-1(-) A549 cells. Vitamin A 56-63 retinol binding protein 1 Homo sapiens 90-99 26807202-9 2015 At >1muM concentrations, all trans-retinoic acid and retinol reduced viability more in CRBP-1(+) than in CRBP-1(-) A549 cells. Vitamin A 56-63 retinol binding protein 1 Homo sapiens 90-96 25879031-6 2015 CRBP-1 acts as chaperone and regulates the uptake, subsequent esterification, and bioavailability of retinol. Vitamin A 101-108 retinol binding protein 1 Homo sapiens 0-6 25879031-10 2015 Reexpression of CRBP-1 increased retinol sensitivity and reduced viability of ovarian cancer cells in vitro. Vitamin A 33-40 retinol binding protein 1 Homo sapiens 16-22 25879031-11 2015 Further studies are needed to explore new therapeutic strategies aimed at restoring CRBP-1-mediated intracellular retinol trafficking and the meaning of CRBP-1 expression in cancer patients" screening for a more personalized and efficacy retinoid therapy. Vitamin A 114-121 retinol binding protein 1 Homo sapiens 84-90 25478840-2 2014 Although structures of retinol-bound CRBPI and CRBPII are known, no structure of a retinal-bound CRBP has been reported. Vitamin A 23-30 retinol binding protein 1 Homo sapiens 37-42 25478840-2 2014 Although structures of retinol-bound CRBPI and CRBPII are known, no structure of a retinal-bound CRBP has been reported. Vitamin A 23-30 retinol binding protein 1 Homo sapiens 37-41