PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 34991674-3 2022 We used Bufalin as a probe to screen its potential targets by proteome microarray, in which AHSA1 was the unique target of Bufalin. bufalin 8-15 activator of HSP90 ATPase activity 1 Homo sapiens 92-97 34991674-3 2022 We used Bufalin as a probe to screen its potential targets by proteome microarray, in which AHSA1 was the unique target of Bufalin. bufalin 123-130 activator of HSP90 ATPase activity 1 Homo sapiens 92-97 34991674-5 2022 The potential mechanisms of Bufalin and KU-177 in AHSA1/HSP90 were verified by co-immunoprecipitation, mass spectrometry, site mutation and microscale thermophoresis assay. bufalin 28-35 activator of HSP90 ATPase activity 1 Homo sapiens 50-55 34991674-9 2022 Additionally, we identified AHSA1-K137 as the specific binding site of Bufalin on AHSA1, mutation of which decreased the interaction of AHSA1 with HSP90A and suppressed the function of AHSA1 on mediating CDK6 and PSMD2. bufalin 71-78 activator of HSP90 ATPase activity 1 Homo sapiens 28-33 34991674-9 2022 Additionally, we identified AHSA1-K137 as the specific binding site of Bufalin on AHSA1, mutation of which decreased the interaction of AHSA1 with HSP90A and suppressed the function of AHSA1 on mediating CDK6 and PSMD2. bufalin 71-78 activator of HSP90 ATPase activity 1 Homo sapiens 82-87 34991674-9 2022 Additionally, we identified AHSA1-K137 as the specific binding site of Bufalin on AHSA1, mutation of which decreased the interaction of AHSA1 with HSP90A and suppressed the function of AHSA1 on mediating CDK6 and PSMD2. bufalin 71-78 activator of HSP90 ATPase activity 1 Homo sapiens 136-141 34991674-9 2022 Additionally, we identified AHSA1-K137 as the specific binding site of Bufalin on AHSA1, mutation of which decreased the interaction of AHSA1 with HSP90A and suppressed the function of AHSA1 on mediating CDK6 and PSMD2. bufalin 71-78 activator of HSP90 ATPase activity 1 Homo sapiens 185-190