PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15220355-0 2004 Regulation of insulin action by ceramide: dual mechanisms linking ceramide accumulation to the inhibition of Akt/protein kinase B. Ceramides 32-40 protein tyrosine kinase 2 beta Homo sapiens 113-129 19303901-3 2009 Ceramide, the most simple sphingolipid, directly inhibits phosphorylation of the insulin signaling mediator Akt/Protein Kinase B. Ceramides 0-8 protein tyrosine kinase 2 beta Homo sapiens 112-128 18996148-4 2009 The early inhibition of the neuronal survival pathway regulated by phosphatidil-inositol-3-kinase/protein kinase B or AKT mediated by ceramide may be a relevant early event in the decision of neuronal survival/death. Ceramides 134-142 protein tyrosine kinase 2 beta Homo sapiens 98-114 15220355-0 2004 Regulation of insulin action by ceramide: dual mechanisms linking ceramide accumulation to the inhibition of Akt/protein kinase B. Ceramides 66-74 protein tyrosine kinase 2 beta Homo sapiens 113-129 12750385-16 2003 This in turn prevents ceramide-induced down-regulation of PKB, the activity of which is required to maintain production of Bcl-XL. Ceramides 22-30 protein tyrosine kinase 2 beta Homo sapiens 58-61 14970205-0 2004 Ceramide-mediated macroautophagy involves inhibition of protein kinase B and up-regulation of beclin 1. Ceramides 0-8 protein tyrosine kinase 2 beta Homo sapiens 56-72 14970205-4 2004 Ceramide reverted the interleukin 13-dependent inhibition of macroautophagy by interfering with the activation of protein kinase B. Ceramides 0-8 protein tyrosine kinase 2 beta Homo sapiens 114-130 14560023-0 2003 Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism. Ceramides 0-8 protein tyrosine kinase 2 beta Homo sapiens 82-98 14560023-0 2003 Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism. Ceramides 0-8 protein tyrosine kinase 2 beta Homo sapiens 100-103 14560023-2 2003 Recent evidence indicates that ceramide may regulate these responses by inhibiting the stimulus-mediated activation of protein kinase B (PKB), a key determinant of cell fate and insulin action. Ceramides 31-39 protein tyrosine kinase 2 beta Homo sapiens 119-135 14560023-2 2003 Recent evidence indicates that ceramide may regulate these responses by inhibiting the stimulus-mediated activation of protein kinase B (PKB), a key determinant of cell fate and insulin action. Ceramides 31-39 protein tyrosine kinase 2 beta Homo sapiens 137-140 14560023-5 2003 However, dissociation of the kinase complex and the attendant hormonal activation of PKB were prevented by ceramide. Ceramides 107-115 protein tyrosine kinase 2 beta Homo sapiens 85-88 14560023-6 2003 Under these circumstances, ceramide activated PKCzeta, leading to phosphorylation of the PKB-PH domain on Thr(34). Ceramides 27-35 protein tyrosine kinase 2 beta Homo sapiens 89-92 14560023-8 2003 In contrast, a PKB-PH domain with a T34A mutation retained the ability to bind PIP(3) even in the presence of a ceramide-activated PKCzeta and, as such, expression of PKB T34A mutant in L6 cells was resistant to inhibition by ceramide treatment. Ceramides 112-120 protein tyrosine kinase 2 beta Homo sapiens 15-18 14560023-8 2003 In contrast, a PKB-PH domain with a T34A mutation retained the ability to bind PIP(3) even in the presence of a ceramide-activated PKCzeta and, as such, expression of PKB T34A mutant in L6 cells was resistant to inhibition by ceramide treatment. Ceramides 112-120 protein tyrosine kinase 2 beta Homo sapiens 167-170 14560023-8 2003 In contrast, a PKB-PH domain with a T34A mutation retained the ability to bind PIP(3) even in the presence of a ceramide-activated PKCzeta and, as such, expression of PKB T34A mutant in L6 cells was resistant to inhibition by ceramide treatment. Ceramides 226-234 protein tyrosine kinase 2 beta Homo sapiens 15-18 14560023-8 2003 In contrast, a PKB-PH domain with a T34A mutation retained the ability to bind PIP(3) even in the presence of a ceramide-activated PKCzeta and, as such, expression of PKB T34A mutant in L6 cells was resistant to inhibition by ceramide treatment. Ceramides 226-234 protein tyrosine kinase 2 beta Homo sapiens 167-170 14560023-9 2003 Inhibitors of PKCzeta and a kinase-dead PKCzeta both antagonized the inhibitory effect of ceramide on PKB. Ceramides 90-98 protein tyrosine kinase 2 beta Homo sapiens 102-105 14560023-10 2003 Since PKB confers a prosurvival signal and regulates numerous pathways in response to insulin, suppressing its activation by a PKCzeta-dependent process may be one mechanism by which ceramide promotes cell death and induces insulin resistance. Ceramides 183-191 protein tyrosine kinase 2 beta Homo sapiens 6-9 11897496-0 2002 Lithium blocks the PKB and GSK3 dephosphorylation induced by ceramide through protein phosphatase-2A. Ceramides 61-69 protein tyrosine kinase 2 beta Homo sapiens 19-22 12525490-4 2003 Preventing de novo ceramide synthesis negated the antagonistic effect of saturated FFAs toward Akt/protein kinase B. Ceramides 19-27 protein tyrosine kinase 2 beta Homo sapiens 99-115 12569071-0 2003 Interleukin-1beta inhibits ATP-induced protein kinase B activation in renal mesangial cells by two different mechanisms: the involvement of nitric oxide and ceramide. Ceramides 157-165 protein tyrosine kinase 2 beta Homo sapiens 39-55 12569071-11 2003 5 In summary, our data show that IL-1beta exerts both short-term and long-term inhibitory effects on ATP-stimulated PKB activation, the short-term effect probably involves ceramide formation, whereas the long-term effect is due to inducible NO synthase induction and subsequent NO formation. Ceramides 172-180 protein tyrosine kinase 2 beta Homo sapiens 116-119 12569071-12 2003 These results reveal a further facet in the mechanisms of ceramide- and NO-induced cell death, i.e. by turning off the survival signal elicited by PKB. Ceramides 58-66 protein tyrosine kinase 2 beta Homo sapiens 147-150 12213802-0 2002 Ceramide and reactive oxygen species generated by H2O2 induce caspase-3-independent degradation of Akt/protein kinase B. Ceramides 0-8 protein tyrosine kinase 2 beta Homo sapiens 103-119 11706021-6 2002 However, strikingly, the ceramide-induced non-apoptotic cell death was inhibited by the activation of the Akt/protein kinase B pathway through the expression of a constitutively active version of Akt. Ceramides 25-33 protein tyrosine kinase 2 beta Homo sapiens 110-126 11574400-2 2001 Ceramide is known to activate both atypical protein kinase C (aPKC) zeta and protein phosphatase (PP) 2A, and each of these effectors has been reported to inhibit PKB. Ceramides 0-8 protein tyrosine kinase 2 beta Homo sapiens 163-166 11171115-4 2001 Here we show that ceramide analogues specifically prevent the recruitment of the PtdIns(3,4,5)P(3)-binding proteins Akt/protein kinase B (PKB) or the general receptor for phosphoinositides-1 (GRP1). Ceramides 18-26 protein tyrosine kinase 2 beta Homo sapiens 120-136 11270673-0 2001 Ceramide impairs the insulin-dependent membrane recruitment of protein kinase B leading to a loss in downstream signalling in L6 skeletal muscle cells. Ceramides 0-8 protein tyrosine kinase 2 beta Homo sapiens 63-79 11270673-7 2001 Expression of a membrane-targetted protein kinase B led to its constitutive activation and an increase in glucose transport that was not inhibited by ceramide. Ceramides 150-158 protein tyrosine kinase 2 beta Homo sapiens 35-51 11270673-8 2001 CONCLUSIONS/INTERPRETATION: These findings suggest that a defect in protein kinase B recruitment underpins the ceramide-induced loss in insulin sensitivity of key cell responses such as glucose transport and glycogen synthesis in L6 cells. Ceramides 111-119 protein tyrosine kinase 2 beta Homo sapiens 68-84 10788440-0 2000 Ceramide inhibits protein kinase B/Akt by promoting dephosphorylation of serine 473. Ceramides 0-8 protein tyrosine kinase 2 beta Homo sapiens 18-34 10788440-3 2000 We have examined in detail the relationship between ceramide signaling and the activation of an important signaling pathway, phosphatidylinositol (PI) 3-kinase and its downstream target, protein kinase B (PKB). Ceramides 52-60 protein tyrosine kinase 2 beta Homo sapiens 187-203 10788440-3 2000 We have examined in detail the relationship between ceramide signaling and the activation of an important signaling pathway, phosphatidylinositol (PI) 3-kinase and its downstream target, protein kinase B (PKB). Ceramides 52-60 protein tyrosine kinase 2 beta Homo sapiens 205-208 10788440-5 2000 Addition of cell-permeable ceramide analogs, C(2)- or C(6)-ceramide, caused a partial loss (50-60%) of PKB activation. Ceramides 27-35 protein tyrosine kinase 2 beta Homo sapiens 103-106 10467260-0 1999 Overexpression of Akt (protein kinase B) confers protection against apoptosis and prevents formation of ceramide in response to pro-apoptotic stimuli. Ceramides 104-112 protein tyrosine kinase 2 beta Homo sapiens 23-39 34962430-8 2022 Ceramides prevented insulin-induced phosphorylation of PKB and NDRG1, but not of Nedd4-2. Ceramides 0-9 protein tyrosine kinase 2 beta Homo sapiens 55-58