PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 16835396-7 2006 The 24-h stimulation of NCI-H295R cells with forskolin increased the expression of steroidogenic acute regulatory protein (StAR), CYP17, CYP21A2, and CYP11A1, whereas only StAR mRNA expression was increased significantly by incubation with DHEA-S. Colforsin 45-54 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 130-135 23415678-9 2013 Only the cAMP inducer forskolin induced CYP17 activity, while CYP19 was induced by four test compounds in the H295R assay. Colforsin 22-31 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 40-45 21733996-6 2011 RESULTS: FSK treatment amplified CYP17 mRNA levels in both cell types when compared with basal, with levels increasing over time, peaking at 72 h, and appearing more robust in primary cells; this difference ranged from 2- to 10-fold and was statistically significant at all time points. Colforsin 9-12 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 33-38 11532475-10 2001 Northern analysis revealed a significant decrease in the expression of CYP17 mRNA in forskolin-stimulated cells treated with metformin (200 microM) compared with forskolin-only-treated cells, however, there was no significant change in steroidogenic acute regulatory protein mRNA expression. Colforsin 85-94 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 71-76 15598676-6 2005 Forskolin treatment also prolonged CYP17 mRNA half-life in both normal and PCOS theca cells. Colforsin 0-9 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 35-40 15598676-7 2005 In vitro mRNA degradation studies demonstrated that the 5"-untranslated region confers increased stability to CYP17 mRNA in PCOS theca cells and showed that the 5"-untranslated region of CYP17 also confers forskolin-stimulated stabilization of CYP17 mRNA. Colforsin 206-215 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 187-192 15598676-7 2005 In vitro mRNA degradation studies demonstrated that the 5"-untranslated region confers increased stability to CYP17 mRNA in PCOS theca cells and showed that the 5"-untranslated region of CYP17 also confers forskolin-stimulated stabilization of CYP17 mRNA. Colforsin 206-215 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 187-192 15666819-3 2004 To this end we isolated marmoset and rhesus adrenocortical cells, and treated the cells with known regulators of P450c17 expression for 48 h. P450c17 protein increased with Forskolin (F) treatment, but was marginally inhibited by AII (A) and TPA (T) alone. Colforsin 173-182 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 142-149 15128284-5 2004 Whereas expression of CYP17 mRNA and protein was up regulated in the presence of forskolin and forskolin in combination with insulin. Colforsin 81-90 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 22-27 15128284-5 2004 Whereas expression of CYP17 mRNA and protein was up regulated in the presence of forskolin and forskolin in combination with insulin. Colforsin 95-104 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 22-27 14576182-6 2004 Western analyses demonstrated that VPA treatment increased both basal and forskolin-stimulated P450c17 and P450scc protein levels, whereas the amount of steroidogenic acute regulatory protein was unaffected. Colforsin 74-83 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 95-102 15625562-6 2004 We also demonstrated that in H295R cells TGF-beta inhibited both basal and forskolin-stimulated accumulation of CYP17 mRNA. Colforsin 75-84 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 112-117 11532475-8 2001 Western blot analysis revealed that metformin significantly inhibited the expression of steroidogenic acute regulatory (StAR) protein and 17 alpha-hydroxylase (CYP17) expression in cells stimulated with forskolin compared with forskolin treatment alone. Colforsin 203-212 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 160-165 11532475-10 2001 Northern analysis revealed a significant decrease in the expression of CYP17 mRNA in forskolin-stimulated cells treated with metformin (200 microM) compared with forskolin-only-treated cells, however, there was no significant change in steroidogenic acute regulatory protein mRNA expression. Colforsin 162-171 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 71-76 10379893-5 1999 Forskolin-stimulated cholesterol side chain cleavage enzyme (CYP11A) and 17alpha-hydroxylase/17,20-desmolase (CYP17) expression were augmented in PCOS theca cells compared with normal cells, whereas no differences were found in steroidogenic acute regulatory protein mRNA expression. Colforsin 0-9 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 110-115 10852468-4 2000 Cotransfection of a steroidogenic factor-1 expression plasmid was found to be required for detection of basal and forskolin-stimulated CYP17 promoter activity but not for StAR promoter activity. Colforsin 114-123 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 135-140 10852468-7 2000 Basal and forskolin-stimulated CYP17 promoter activity was 4-fold greater in PCOS cells than in theca cells isolated from normal ovaries. Colforsin 10-19 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 31-36 10999829-13 2000 Forskolin treatment led to an increase in CYP17, CYP11A1, 3betaHSD, and StAR protein expression. Colforsin 0-9 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 42-47 10999829-14 2000 BMP-4 markedly inhibited forskolin stimulation of CYP17 expression but had little effect on 3betaHSD, CYP11A1, or StAR protein levels. Colforsin 25-34 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 50-55 10999829-16 2000 In addition, BMP-4 inhibited basal and forskolin stimulation of CYP17 messenger RNA expression as determined by RNase protection assay. Colforsin 39-48 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 64-69 10999829-19 2000 Activin also inhibited forskolin induction of CYP17 protein expression as determined by Western analysis. Colforsin 23-32 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 46-51 8943800-10 1996 Treatment with forskolin resulted in a dramatic increase in both cortisol and dehydroepiandrosterone (DHEA) secretion together with increases in expression of 3 beta-HSD and P450c17 as measured at the level of mRNA and activity. Colforsin 15-24 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 174-181 8943800-12 1996 Cotreatment with forskolin and All, however, resulted in a dose-dependent reduction in cortisol and DHEA secretion concomitant with a marked attenuation of 3 beta-HSD and P450c17 expression. Colforsin 17-26 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 171-178 8964847-10 1996 Treatment with forskolin (10 mumol/L, 48 h) resulted in a dramatic increase in both cortisol and dehydroepiandrosterone production together with increases in expression of P450c17, P450scc, and 3 beta-HSD as measured at the level of mRNA and activity. Colforsin 15-24 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 172-179 8964847-12 1996 Cotreatment with forskolin and AII, however, resulted in a dose-dependent reduction in cortisol and DHEA production concomitant with a marked attenuation of P450scc and P450c17 expression. Colforsin 17-26 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 169-176 8557160-7 1996 Forskolin stimulated A and P450c17 activity were enhanced by treatment with insulin and the IGFs. Colforsin 0-9 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 27-34 8557160-10 1996 CONCLUSIONS: We observed that forskolin-stimulated human ovarian thecal-like tumor cell steroidogenesis, P450c17, and 3 beta HSD activity, as well as mRNA content for P450scc, 3 beta HSD, and P450c17. Colforsin 30-39 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 105-112 8557160-10 1996 CONCLUSIONS: We observed that forskolin-stimulated human ovarian thecal-like tumor cell steroidogenesis, P450c17, and 3 beta HSD activity, as well as mRNA content for P450scc, 3 beta HSD, and P450c17. Colforsin 30-39 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 192-199 8550761-15 1996 Treatment of HOTT cells with forskolin caused a time-dependent induction of the messenger RNAs for cholesterol side-chain cleavage P450, 3 beta-hydroxysteroid dehydrogenase, and P450c17. Colforsin 29-38 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 178-185 8623837-6 1996 Forskolin treatment significantly stimulated steroid production and the enzymatic activity of P450c17 and 3 beta HSD up to 10-fold above basal levels. Colforsin 0-9 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 94-101 8623837-8 1996 Forskolin-stimulated immunodetectable P450c17 alpha protein was markedly inhibited by transforming growth factor-beta 1. Colforsin 0-9 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 38-45 8623837-9 1996 In addition, transforming growth factor-beta 1 markedly inhibited the forskolin-stimulation of P450c17 messenger ribonucleic acid, while not significantly altering P450scc or 3 beta HSD messenger ribonucleic acid expression. Colforsin 70-79 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 95-102 8623837-10 1996 CONCLUSION: Forskolin stimulated human ovarian thecal-like tumor cell steroidogenesis, P450c17 and 3 beta HSD activity, immunodetectable P450c17, and messenger ribonucleic acid content for P450scc, P450c17, and 3 beta HSD. Colforsin 12-21 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 87-100 8623837-10 1996 CONCLUSION: Forskolin stimulated human ovarian thecal-like tumor cell steroidogenesis, P450c17 and 3 beta HSD activity, immunodetectable P450c17, and messenger ribonucleic acid content for P450scc, P450c17, and 3 beta HSD. Colforsin 12-21 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 87-94 8623837-10 1996 CONCLUSION: Forskolin stimulated human ovarian thecal-like tumor cell steroidogenesis, P450c17 and 3 beta HSD activity, immunodetectable P450c17, and messenger ribonucleic acid content for P450scc, P450c17, and 3 beta HSD. Colforsin 12-21 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 137-144 8623837-11 1996 Transforming growth factor-beta 1 inhibited forskolin stimulation of androstenedione production through the inhibition of P450c17 expression. Colforsin 44-53 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 122-129 8699135-7 1996 Forskolin (10 microM) markedly increased production of both androstenedione (fivefold) and progesterone (threefold) as well as the activity of 3 beta HSD (sevenfold), and P450c17 (sevenfold) over basal. Colforsin 0-9 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 171-178 8699135-8 1996 Forskolin stimulated the expression of mRNA for P450scc (fourfold), 3 beta HSD (threefold), and P450c17 (eightfold) over basal. Colforsin 0-9 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 96-103 8699135-10 1996 This was attributed to a reduction of P450c17 mRNA (45% of forskolin alone) and activity (45% of forskolin alone). Colforsin 59-68 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 38-45 8699135-10 1996 This was attributed to a reduction of P450c17 mRNA (45% of forskolin alone) and activity (45% of forskolin alone). Colforsin 97-106 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 38-45 8699135-13 1996 HOTT cell responses to activin (0.05-50 ng/ml) and inhibin (0.05-50 ng/ml) in the presence of forskolin demonstrated dose-dependent effects on the steroid accumulation, enzymatic activity and mRNA expression of P450c17. Colforsin 94-103 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 211-218 8597483-5 1995 Agents that act by increasing intracellular calcium (angiotensin II and K+ ions) as well as protein kinase A pathway activators (ACTH, forskolin, and dibutyryl cAMP) individually increased the mRNA levels and activity of P450c17. Colforsin 135-144 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 221-228 33028758-4 2021 After FSK induction, mRNA levels of StAR and CYP11B2 were highest at 12 hours and CYP17 mRNA level reached a peak at 6 hours, but HSD3B1 mRNA level was transiently decreased at 3 hours. Colforsin 6-9 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 82-87 33028758-6 2021 Knockdown of Clock gene by siRNA led to a significant reduction of FSK-induced expression of StAR and CYP17 after 12-h treatment with FSK. Colforsin 67-70 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 102-107 33028758-6 2021 Knockdown of Clock gene by siRNA led to a significant reduction of FSK-induced expression of StAR and CYP17 after 12-h treatment with FSK. Colforsin 134-137 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 102-107