PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 29377304-1 2018 In human trophoblast cells, cyclic AMP or its inducer forskolin (FSK) activates two downstream signaling molecules, protein kinase A (PKA) and exchange protein directly activated by cAMP (EPAC), both of which induce syncytialization, cell fusion, and the production of human chorionic gonadotropin (hCG) and progesterone. Colforsin 54-63 Rap guanine nucleotide exchange factor 3 Homo sapiens 188-192 31864907-4 2020 From a screening of signaling pathway modulators, we confirmed that forskolin facilitated efficient and rapid iEC reprogramming via activation of the cyclic AMP (cAMP)/exchange proteins directly activated by cAMP (EPAC)/RAP1 axis. Colforsin 68-77 Rap guanine nucleotide exchange factor 3 Homo sapiens 214-218 30717378-7 2019 The Epac sensor showed a more rapid response to pharmaceutical agents, e.g., Forskolin or the A2B adenosine receptor agonist NECA, in close proximity to the plasma membrane compared to the cytosol. Colforsin 77-86 Rap guanine nucleotide exchange factor 3 Homo sapiens 4-8 31255721-4 2019 Elevation of cAMP using forskolin, cAMP-analogues or adenosine A2B-receptor activation significantly reduced EPAC1 mRNA and protein levels and inhibited formation of F-actin stress fibres. Colforsin 24-33 Rap guanine nucleotide exchange factor 3 Homo sapiens 109-114 31255721-9 2019 Chromatin-immunoprecipitation detected a significant reduction in histone3-lysine27-acetylation at the EPAC1 proximal promoter in response to forskolin stimulation. Colforsin 142-151 Rap guanine nucleotide exchange factor 3 Homo sapiens 103-108 31255721-10 2019 HDAC1/3 inhibition partially reversed forskolin inhibition of EPAC1 expression, which was completely rescued by simultaneously expressing constitutively active YAP. Colforsin 38-47 Rap guanine nucleotide exchange factor 3 Homo sapiens 62-67 29377304-1 2018 In human trophoblast cells, cyclic AMP or its inducer forskolin (FSK) activates two downstream signaling molecules, protein kinase A (PKA) and exchange protein directly activated by cAMP (EPAC), both of which induce syncytialization, cell fusion, and the production of human chorionic gonadotropin (hCG) and progesterone. Colforsin 65-68 Rap guanine nucleotide exchange factor 3 Homo sapiens 188-192 28912066-5 2017 In DAF-2-loaded HUVEC monolayers, forskolin, PKA and Epac activators significantly increased NO release, and the forskolin effect was reduced by inhibition of PKA (Rp-cAMPs), Epac (ESI-09), eNOS (L-NAME) or phosphoinositide 3-kinase (PI3K; LY-294,002). Colforsin 34-43 Rap guanine nucleotide exchange factor 3 Homo sapiens 175-179 28912066-5 2017 In DAF-2-loaded HUVEC monolayers, forskolin, PKA and Epac activators significantly increased NO release, and the forskolin effect was reduced by inhibition of PKA (Rp-cAMPs), Epac (ESI-09), eNOS (L-NAME) or phosphoinositide 3-kinase (PI3K; LY-294,002). Colforsin 113-122 Rap guanine nucleotide exchange factor 3 Homo sapiens 53-57 28912066-5 2017 In DAF-2-loaded HUVEC monolayers, forskolin, PKA and Epac activators significantly increased NO release, and the forskolin effect was reduced by inhibition of PKA (Rp-cAMPs), Epac (ESI-09), eNOS (L-NAME) or phosphoinositide 3-kinase (PI3K; LY-294,002). Colforsin 113-122 Rap guanine nucleotide exchange factor 3 Homo sapiens 175-179 24533119-7 2014 cAMP with forskolin prevented CB1R-induced synaptic silencing, via activation of the Exchange Protein directly Activated by cAMP (Epac). Colforsin 10-19 Rap guanine nucleotide exchange factor 3 Homo sapiens 85-128 28512254-9 2017 In addition, we found that PKA was also involved in the regulation of angiogenesis in EC since the adenylyl cyclase (AC) activator forskolin (Fsk), but not 8-pCPT, increased sprouting in Epac1-depleted HUVEC and this increase was sensitive to a selective synthetic peptide PKA inhibitor. Colforsin 131-140 Rap guanine nucleotide exchange factor 3 Homo sapiens 187-192 28512254-9 2017 In addition, we found that PKA was also involved in the regulation of angiogenesis in EC since the adenylyl cyclase (AC) activator forskolin (Fsk), but not 8-pCPT, increased sprouting in Epac1-depleted HUVEC and this increase was sensitive to a selective synthetic peptide PKA inhibitor. Colforsin 142-145 Rap guanine nucleotide exchange factor 3 Homo sapiens 187-192 25220136-11 2015 A similar Epac-mediated pathway was seen following beta2 -adrenoceptor or forskolin stimulation. Colforsin 74-83 Rap guanine nucleotide exchange factor 3 Homo sapiens 10-14 27500354-5 2016 Measurement of intracellular cyclic adenosine monophosphate (cAMP) levels is demonstrated in chondrocytes under forskolin stimulation using a probe for EPAC1 activation (ICUE1) and the ability to detect differences in cAMP signaling dependent on hydrogel material type, herein a photocrosslinking hydrogel (PC-gel, polyethylene glycol dimethacrylate) and a thermoresponsive hydrogel (TR-gel). Colforsin 112-121 Rap guanine nucleotide exchange factor 3 Homo sapiens 152-157 25913012-4 2015 In addition, co-activation of EPAC1 and PKA, with a combination of the adenylate cyclase activator, forskolin, and the cAMP phosphodiesterase inhibitor, rolipram, was found to synergistically enhance cell spreading, in association with cortical actin bundling and mobilisation of ezrin to the plasma membrane. Colforsin 100-109 Rap guanine nucleotide exchange factor 3 Homo sapiens 30-35 24469067-6 2014 Forskolin, dibutyryl cAMP (a cAMP analogue), 6-Bnz-cAMP and 8-pCPT-2"-O-Me-cAMP increased NO levels in endothelial cells and the forskolin effect was significantly inhibited by inactivation of both Epac and PKA, and eNOS inhibition. Colforsin 0-9 Rap guanine nucleotide exchange factor 3 Homo sapiens 198-202 24469067-6 2014 Forskolin, dibutyryl cAMP (a cAMP analogue), 6-Bnz-cAMP and 8-pCPT-2"-O-Me-cAMP increased NO levels in endothelial cells and the forskolin effect was significantly inhibited by inactivation of both Epac and PKA, and eNOS inhibition. Colforsin 129-138 Rap guanine nucleotide exchange factor 3 Homo sapiens 198-202 24469067-7 2014 Our results indicate that the endothelium-dependent component of forskolin/cAMP-induced vasorelaxation is partially mediated by an increase in endothelial NO release due to an enhanced eNOS activity through PKA and Epac activation in endothelial cells. Colforsin 65-74 Rap guanine nucleotide exchange factor 3 Homo sapiens 215-219 24533119-7 2014 cAMP with forskolin prevented CB1R-induced synaptic silencing, via activation of the Exchange Protein directly Activated by cAMP (Epac). Colforsin 10-19 Rap guanine nucleotide exchange factor 3 Homo sapiens 130-134 20663796-14 2010 Knockdown of Epac1 or Rap1 repressed the Db-, CPT- or forskolin-induced cell fusion. Colforsin 54-63 Rap guanine nucleotide exchange factor 3 Homo sapiens 13-18 24036110-4 2013 We found that 8-pCPT-2"-O-Me-cAMP, a specific activator of the exchange protein directly activated by cAMP (Epac), mimicked and occluded forskolin-induced potentiation of glutamate release. Colforsin 137-146 Rap guanine nucleotide exchange factor 3 Homo sapiens 108-112 23473803-6 2013 Employing a FRET-based biosensor constructed from the nucleotide binding domain of the type 1 exchange protein activated by cAMP, EPAC1, we demonstrated that moexipril markedly potentiated the ability of forskolin to increase intracellular cAMP levels. Colforsin 204-213 Rap guanine nucleotide exchange factor 3 Homo sapiens 130-135 22227470-9 2012 Co-treatment with rolipram and forskolin also enhanced CREB phosphorylation on serine 133 that was inhibited by H-89, PKA inhibitor and cAMP-responsive guanine nucleotide exchange factor 1(Epac1), a Rap GDP exchange factor-mediated Rap1 activity in A172 cells. Colforsin 31-40 Rap guanine nucleotide exchange factor 3 Homo sapiens 189-194 20032304-6 2010 These effects of forskolin were mimicked by an activator for Epac but not by that for protein kinase A. Colforsin 17-26 Rap guanine nucleotide exchange factor 3 Homo sapiens 61-65 20038525-0 2010 Epac1 mediates protein kinase A-independent mechanism of forskolin-activated intestinal chloride secretion. Colforsin 57-66 Rap guanine nucleotide exchange factor 3 Homo sapiens 0-5 20038525-3 2010 Therefore, we tested whether both PKA and Epac are involved in forskolin (FSK)/cAMP-stimulated Cl- secretion. Colforsin 63-72 Rap guanine nucleotide exchange factor 3 Homo sapiens 42-46 20038525-8 2010 In contrast, T84 cells with silenced Epac1 had a reduced I(sc) response to FSK, and this response was completely inhibited by H89, but not by the phospholipase C inhibitor U73122 or BAPTA-AM. Colforsin 75-78 Rap guanine nucleotide exchange factor 3 Homo sapiens 37-42 19385062-10 2009 These results suggest that forskolin stimulates angiogenesis through coordinated cross-talk between two distinct pathways, PKA-dependent VEGF expression and Epac-dependent ERKactivation and PI3K/Akt/eNOS/NO signaling. Colforsin 27-36 Rap guanine nucleotide exchange factor 3 Homo sapiens 157-161 19385062-0 2009 Forskolin increases angiogenesis through the coordinated cross-talk of PKA-dependent VEGF expression and Epac-mediated PI3K/Akt/eNOS signaling. Colforsin 0-9 Rap guanine nucleotide exchange factor 3 Homo sapiens 106-110 19385062-9 2009 Small interfering RNA-mediated knockdown of Epac1 suppressed forskolin-induced angiogenesis and phosphorylation of ERK, Akt, and eNOS, but not CREB phosphorylation and VEGF expression. Colforsin 61-70 Rap guanine nucleotide exchange factor 3 Homo sapiens 44-49 19472214-5 2009 These effects were reversed by addition of forskolin/rolipram (F/R) to increase intracellular cAMP but not by the cAMP analogue 8-pCPT-2"-O-Methyl-cAMP (O-Me-cAMP) which primarily stimulates protein kinase A (PKA)-independent signaling via Epac/Rap 1. Colforsin 43-52 Rap guanine nucleotide exchange factor 3 Homo sapiens 240-244 16172130-0 2005 Coordinate regulation of forskolin-induced cellular proliferation in macrophages by protein kinase A/cAMP-response element-binding protein (CREB) and Epac1-Rap1 signaling: effects of silencing CREB gene expression on Akt activation. Colforsin 25-34 Rap guanine nucleotide exchange factor 3 Homo sapiens 150-155 17565009-11 2007 Overexpression of Epac1 enhanced A(2B)AR-mediated and forskolin-induced ERK1/2 phosphorylation, whereas response to VEGF was unaffected. Colforsin 54-63 Rap guanine nucleotide exchange factor 3 Homo sapiens 18-23 17565009-12 2007 Inhibition of Epac1 expression with small interfering RNA substantially reduced A(2B)AR-mediated and forskolin-induced ERK1/2 phosphorylation and abolished that by 8CPT-2Me-cAMP. Colforsin 101-110 Rap guanine nucleotide exchange factor 3 Homo sapiens 14-19 19176384-11 2009 Collectively, these data show that forskolin strongly inhibits VM through PKA-independent activation of Epac/Rap1, PKA-, and Epac-independent inactivation of ERK1/2 and inhibition of PI3K/Akt. Colforsin 35-44 Rap guanine nucleotide exchange factor 3 Homo sapiens 104-108 19176384-11 2009 Collectively, these data show that forskolin strongly inhibits VM through PKA-independent activation of Epac/Rap1, PKA-, and Epac-independent inactivation of ERK1/2 and inhibition of PI3K/Akt. Colforsin 35-44 Rap guanine nucleotide exchange factor 3 Homo sapiens 125-129 18495429-4 2008 Recently, we reported PKA-independent activation of the protein kinase Akt as well co-immunoprecipitation of Epac1 with Rap1, p-Akt(Thr-308), and p-Akt(Ser-473) in forskolin-stimulated macrophages. Colforsin 164-173 Rap guanine nucleotide exchange factor 3 Homo sapiens 109-114 16172130-10 2005 We conclude that forskolin mediates cellular proliferation via cAMP-dependent activation of both PKA and Epac. Colforsin 17-26 Rap guanine nucleotide exchange factor 3 Homo sapiens 105-109 16172130-1 2005 In this study, we have examined the role of two cAMP downstream effectors protein kinase A (PKA) and Epac, in forskolin-induced macrophage proliferation. Colforsin 110-119 Rap guanine nucleotide exchange factor 3 Homo sapiens 101-105 16172130-5 2005 Incubation of macrophages with forskolin also increased Epac1 and Rap1.GTP. Colforsin 31-40 Rap guanine nucleotide exchange factor 3 Homo sapiens 56-61 16172130-6 2005 Immunoprecipitation of Epac1 in forskolin-stimulated cells co-immunoprecipitated Rap1, p-Akt(Thr-308), and p-Akt(Ser-473). Colforsin 32-41 Rap guanine nucleotide exchange factor 3 Homo sapiens 23-28 15525573-9 2004 Under basal conditions, cAMP-GEFI expression increased progressively throughout culture, and this could be further enhanced when cells were incubated with increasing doses of LH and forskolin. Colforsin 182-191 Rap guanine nucleotide exchange factor 3 Homo sapiens 24-33 15525573-11 2004 The results show that increases in cAMP generated by LH and forskolin, in addition to activating PKA, also induce increases in cAMP-GEFI protein expression in luteinizing human granulosa cells. Colforsin 60-69 Rap guanine nucleotide exchange factor 3 Homo sapiens 127-136 14615375-6 2004 The EPAC-specific cAMP analog 8CPT-2Me-cAMP (8-(4-chloro-phenylthio)-2"-O-methyladenosine-3",5"-cAMP) activates Rap1 in B-CLL cells, but, unlike rolipram/forskolin or 8-Bromo-cAMP, it does not induce PKA activation, as judged by phosphorylation of the transcription factor cAMP-response element binding protein (CREB). Colforsin 154-163 Rap guanine nucleotide exchange factor 3 Homo sapiens 4-8