PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15581358-7 2004 We show that 7C1b-S inhibits Gsalpha-stimulated and Gsalpha-forskolin stimulated activity in our soluble ACVII model system. Colforsin 60-69 GNAS complex locus Homo sapiens 52-59 12831842-9 2003 In addition, AVP treatment also enhanced the expression of Gsalpha protein and Gsalpha-mediated stimulation of adenylyl cyclase by GTPgammaS, N-ethylcarboxamide adenosine (NECA), and forskolin (FSK), whereas the levels of Gbeta were not altered by AVP treatment. Colforsin 194-197 GNAS complex locus Homo sapiens 59-66 12831842-9 2003 In addition, AVP treatment also enhanced the expression of Gsalpha protein and Gsalpha-mediated stimulation of adenylyl cyclase by GTPgammaS, N-ethylcarboxamide adenosine (NECA), and forskolin (FSK), whereas the levels of Gbeta were not altered by AVP treatment. Colforsin 183-192 GNAS complex locus Homo sapiens 59-66 12831842-9 2003 In addition, AVP treatment also enhanced the expression of Gsalpha protein and Gsalpha-mediated stimulation of adenylyl cyclase by GTPgammaS, N-ethylcarboxamide adenosine (NECA), and forskolin (FSK), whereas the levels of Gbeta were not altered by AVP treatment. Colforsin 183-192 GNAS complex locus Homo sapiens 79-86 12831842-9 2003 In addition, AVP treatment also enhanced the expression of Gsalpha protein and Gsalpha-mediated stimulation of adenylyl cyclase by GTPgammaS, N-ethylcarboxamide adenosine (NECA), and forskolin (FSK), whereas the levels of Gbeta were not altered by AVP treatment. Colforsin 194-197 GNAS complex locus Homo sapiens 79-86 9268376-3 1997 In the presence of Gsalpha, adenylyl cyclase is activated in response to occupation of only one forskolin-binding site. Colforsin 96-105 GNAS complex locus Homo sapiens 19-26 10355282-6 1999 Nine (AC-1-AC-9) mammalian enzymes are stimulated by an "alpha" subunit of Gs-protein (Gs alpha) and by the diterpene forskolin, albeit to varying degrees (with AC-9 being least sensitive to forskolin). Colforsin 191-200 GNAS complex locus Homo sapiens 87-95 10786926-4 2000 The amount of alpha subunit of stimulatory GTP-binding protein (GSalpha) and adenylyl cyclase activity in response to forskolin were not different in the two cell lines. Colforsin 118-127 GNAS complex locus Homo sapiens 64-71 9415709-7 1997 Furthermore, high affinity [3H]forskolin binding experiments demonstrated that depalmitoylated Gs alpha is able to associated directly with AC during the state of opioid dependence even without preceding receptor activation. Colforsin 31-40 GNAS complex locus Homo sapiens 95-103 9362361-8 1997 The functional activity of Gs alpha, as determined by adenylyl cyclase (AC) activity, was significantly decreased in tumor as compared to normal liver under both basal and agonist stimulated (guanosine triphosphate gamma S and forskolin) conditions. Colforsin 227-236 GNAS complex locus Homo sapiens 27-35 9228061-3 1997 Based on the three-dimensional structure of C2.forskolin dimer, these three regions (C2 alpha2, C2 alpha3/beta4, and C1 beta1) are close together and form a negatively charged and hydrophobic groove the width of an alpha helix that can accommodate the positively charged adenylyl cyclase binding region of Gsalpha. Colforsin 47-56 GNAS complex locus Homo sapiens 306-313 9268376-5 1997 The affinity of forskolin for adenylyl cyclase is greatly reduced in the absence of Gsalpha ( approximately 40 microM). Colforsin 16-25 GNAS complex locus Homo sapiens 84-91 9193641-2 1997 Persistent treatment (24-48 h) of C6 glioma cells with cholera toxin (100 ng/ml) caused marked downregulation of Gs alpha (75-80%) which could not be mimicked by dibutyryl cAMP (1 mM) and forskolin (10 microM) over the same time periods suggesting that CT-mediated Gs alpha downregulation is independent of cAMP production. Colforsin 188-197 GNAS complex locus Homo sapiens 113-121 8978725-2 1997 The loss in Gs alpha was accompanied by reduced (64.3 +/- 0.35% of control values) NaF-mediated stimulation of adenylyl cyclase and was independent of accumulated cyclic AMP (cAMP) levels, because neither forskolin nor dibutyryl cAMP treatment of cells mimicked the DA-induced effects. Colforsin 205-214 GNAS complex locus Homo sapiens 12-20 8978725-5 1997 Gs alpha mRNA levels were also attenuated (52 +/- 3.5% of control values) by the D1-selective agonist SKF R-38393 but not by forskolin or dibutyryl cAMP. Colforsin 125-134 GNAS complex locus Homo sapiens 0-8 7508389-1 1994 Isoproterenol, forskolin and 8-bromoadenosine 3":5"-cyclic monophosphate increase the level of Gs alpha mRNA in cultured astroglial cells. Colforsin 15-24 GNAS complex locus Homo sapiens 95-103 8663304-4 1996 It is activated synergistically by Gsalpha and forskolin and is inhibited by adenosine (P-site) analogs with kinetic patterns that are identical to those displayed by the native enzymes. Colforsin 47-56 GNAS complex locus Homo sapiens 35-42 8631912-0 1996 Two cytoplasmic domains of mammalian adenylyl cyclase form a Gs alpha- and forskolin-activated enzyme in vitro. Colforsin 75-84 GNAS complex locus Homo sapiens 61-69 8631912-6 1996 The concentration of IIC2-delta 3 needed to complex with IC1 was reduced 10-fold (0.08 microM) when the enzyme was activated by both forskolin and Gs alpha, suggesting that Gs alpha and forskolin increased the affinity of the two cytoplasmic domains for each other. Colforsin 133-142 GNAS complex locus Homo sapiens 173-181 8631912-6 1996 The concentration of IIC2-delta 3 needed to complex with IC1 was reduced 10-fold (0.08 microM) when the enzyme was activated by both forskolin and Gs alpha, suggesting that Gs alpha and forskolin increased the affinity of the two cytoplasmic domains for each other. Colforsin 186-195 GNAS complex locus Homo sapiens 147-155 7792604-2 1995 The soluble enzyme was stimulated by both forskolin and the alpha subunit of the heterotrimeric guanine nucleotide-binding protein (G protein) Gs (Gs alpha). Colforsin 42-51 GNAS complex locus Homo sapiens 147-155 7538952-0 1995 Agonist regulation of high affinity [3H] forskolin binding as a measure of GS alpha-adenylyl cyclase interactions. Colforsin 41-50 GNAS complex locus Homo sapiens 75-83 7523385-6 1994 By contrast, both mutant Gs alpha proteins showed activation of adenylyl cyclase in response to forskolin (10 microM) and fluoroaluminate (10 mM). Colforsin 96-105 GNAS complex locus Homo sapiens 25-33 7508389-7 1994 The culture of astroglial cells with isoproterenol or forskolin (3-24 h) resulted in a transient increase of both the Gs alpha and the Gi alpha protein levels, while the level of G beta subunits was unaffected. Colforsin 54-63 GNAS complex locus Homo sapiens 118-126 7508389-9 1994 The level of Gs alpha mRNA measured by Northern-blot analysis was transiently increased (200%) after stimulation of astroglial cells with isoproterenol or forskolin for an incubation period of 6-9 h, then returned to that of control cells for longer period of time. Colforsin 155-164 GNAS complex locus Homo sapiens 13-21 32105777-4 2020 Surprisingly, in the presence of Gsalpha, the isoform-specific carboxyl-terminal tail of AC9 occludes the forskolin- as well as the active substrate-sites, resulting in marked autoinhibition of the enzyme. Colforsin 106-115 GNAS complex locus Homo sapiens 33-40 1908646-3 1991 This increased Gs alpha-C interaction was the result of an increase in Gs alpha specific activity, as assessed in cyc- reconstitution assays, as well as an increased C activity, as assessed by forskolin-Mn(2+)-stimulated adenylyl cyclase activity. Colforsin 193-202 GNAS complex locus Homo sapiens 15-23 21115086-6 2011 Therefore, as measured immunochemically and by [(3)H]-forskolin binding, there seems to be a vast excess of Gsalpha subunits over catalytic units of AC. Colforsin 54-63 GNAS complex locus Homo sapiens 108-115 17406063-10 2007 On the other hand, Gsalpha-mediated stimulations of adenylyl cyclase by GTPgammaS, isoproterenol and FSK were significantly augmented in 8Br-cGMP-treated cells. Colforsin 101-104 GNAS complex locus Homo sapiens 19-26