PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 16719384-3 2006 A thin-layer chromatographic method is described for the determination of 6beta-hydroxytestosterone formed enzymatically by incubation of [14C]-testosterone with cDNA-expressed CYP3A enzymes or liver microsomes. 6 beta-hydroxytestosterone 74-99 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 177-182 17084830-7 2006 Similar increases and decreases were observed in CYP3A4-specific activity levels as measured using 6beta-hydroxytestosterone formation following incubation with testosterone. 6 beta-hydroxytestosterone 99-124 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 49-55 15037195-7 2004 CYP1A2 and CYP3A4 induction was determined by measuring 7-ethoxyresorufin-O-deethylation activity and 6beta-hydroxytestosterone formation, respectively. 6 beta-hydroxytestosterone 102-127 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 11-17 27699667-3 2006 A thin-layer chromatographic method is described for the determination of 6beta-hydroxytestosterone formed enzymatically by incubation of [14C]-testosterone with cDNA-expressed CYP3A enzymes or liver microsomes. 6 beta-hydroxytestosterone 74-99 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 177-182 15285839-2 2004 We assessed the effect of ethanol on CYP3A-mediated biotransformation using human liver microsomes in-vitro with three prototypic CYP3A-mediated reactions: nifedipine to oxidized nifedipine, triazolam to its 1-hydroxy (1-OH TRZ) and 4-hydroxy (4-OH TRZ) metabolites, and testosterone to 6beta-hydroxytestosterone (6beta-OH TST). 6 beta-hydroxytestosterone 287-312 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 37-42 9531515-1 1998 The major pathway of testosterone oxidation by human liver microsomes is the formation of 6beta-hydroxytestosterone, which is catalyzed by CYP3A4/5 and which accounts for 75-80% of all metabolites formed. 6 beta-hydroxytestosterone 90-115 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 139-145 15621665-2 2004 6beta-hydroxytestosterone production, a marker of CYP3A4 activity, was approximately 3- and 7-fold greater in 1,25(OH)2D3-treated cells compared to untreated cells when incubated with 50 and 500 microM testosterone, respectively, and was unaffected by the addition of digoxin to reduce Pgp activity. 6 beta-hydroxytestosterone 0-25 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 50-56 12814972-3 2003 Recombinant CYP3A4 and CYP3A5 (rCYP3A4 and rCYP3A5) both produced 1-OH and 4-OH metabolites from midazolam and triazolam, 6 beta-hydroxytestosterone from testosterone, and oxidized nifedipine from nifedipine. 6 beta-hydroxytestosterone 122-148 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 12-18 11038151-4 2000 Treatment with silymarin (0.1 and 0.25 mM) significantly reduced the activity of CYP3A4 enzyme (by 50 and 100%, respectively) as determined by the formation of 6-beta-hydroxy testosterone and the activity of uridine diphosphoglucuronosyl transferase (UGT1A6/9) (by 65 and 100%, respectively) as measured by the formation of 4-methylumbelliferone glucuronide. 6 beta-hydroxytestosterone 160-187 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 81-87 10497147-3 1999 We have investigated the effect of TRO on CYP3A protein content and the activity of CYP3A (as measured by the formation of 6beta-hydroxytestosterone formation) in primary cultures of human hepatocytes in comparison with rifampicin (RIF). 6 beta-hydroxytestosterone 123-148 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 84-89 10497147-9 1999 There was a significant correlation (R(2) = 0.89) between the content of immunoreactive CYP3A protein in the hepatocytes and the rate of formation of 6beta-hydroxytestosterone. 6 beta-hydroxytestosterone 150-175 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 88-93 34031539-5 2021 CYP1A2 and CYP3A4 metabolites (acetaminophen and 6beta-hydroxytestosterone, respectively,) in liver microsomes were also significantly decreased. 6 beta-hydroxytestosterone 49-74 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 11-17 31301485-8 2019 Besides absorption, intestinal wall metabolism of testosterone (CYP3A4) was determined by showing a linear formation (R2 = 0.99; up to 165 min) of the main metabolites androstenedione and 6Beta-hydroxytestosterone, indicating no loss of metabolic capacity of the intestinal tissue within the system. 6 beta-hydroxytestosterone 188-213 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 64-70 28986474-1 2017 The metabolism of testosterone to 6beta-hydroxytestosterone (6beta-OH-T) is a commonly used assay to evaluate human CYP3A enzyme activities. 6 beta-hydroxytestosterone 34-59 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 116-121 29375004-7 2019 Predicted contributions of CYP3A4 to formations of 6beta-hydroxytestosterone, 1"-hydroxymidazolam, norverapamil, ortho-hydroxyatorvastatin and para-hydroxyatorvastatin using other probes were 47.46-219.46%, 21.62-98.87%, 186.49-462.44%, 21.87-101.15% and 53.62-247.97%, respectively. 6 beta-hydroxytestosterone 51-76 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 27-33 28867739-7 2017 Metabolism to 6beta-hydroxytestosterone by recombinant CYP3A4 was strongly inhibited by cabbage, onion, and green pepper juices, and cabbage and green pepper juices significantly inhibited CYP3A4 activity in a preincubation time-dependent manner. 6 beta-hydroxytestosterone 14-39 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 55-61 28867739-7 2017 Metabolism to 6beta-hydroxytestosterone by recombinant CYP3A4 was strongly inhibited by cabbage, onion, and green pepper juices, and cabbage and green pepper juices significantly inhibited CYP3A4 activity in a preincubation time-dependent manner. 6 beta-hydroxytestosterone 14-39 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 189-195 26899743-2 2016 Dose-dependent inhibition of CYP3A4-mediated conversion of the model compound, testosterone, to its major metabolite, 6beta-hydroxy testosterone was noted. 6 beta-hydroxytestosterone 118-144 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 29-35 25727956-6 2015 This effect has been confirmed with two CYP3A4 substrates, testosterone (formation of 6beta-hydroxytestosterone) and diazepam (temazepam formation). 6 beta-hydroxytestosterone 86-111 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 40-46 24242708-9 2014 Mass spectrometry revealed that overexpression of CYP3A protein in prostate cancer cells resulted in a significant increase in the oxidative inactivation of testosterone and DHEA to their 6-beta-hydroxy-testosterone and 16-alpha-hydroxy-DHEA metabolites, respectively. 6 beta-hydroxytestosterone 188-215 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 50-55 17581220-6 2007 The CYP3A activity was measured by the amount of 6beta-hydroxytestosterone in the culture medium and CYP3A protein in hepatocyte lysate was semiquantified by immunoblotting. 6 beta-hydroxytestosterone 49-74 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 4-9 27582329-5 2016 The metabolisms to 6beta-hydroxytestosterone by recombinant CYP3A4 were significantly inhibited by VJ-o, VJ-g, and VJ-y in a preincubation time-dependent manner, and CYP3A4 activity in LS180 cells were significantly inhibited by VJ-o and VJ-y. 6 beta-hydroxytestosterone 19-44 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 60-66 27582329-5 2016 The metabolisms to 6beta-hydroxytestosterone by recombinant CYP3A4 were significantly inhibited by VJ-o, VJ-g, and VJ-y in a preincubation time-dependent manner, and CYP3A4 activity in LS180 cells were significantly inhibited by VJ-o and VJ-y. 6 beta-hydroxytestosterone 19-44 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 166-172 22298448-3 2012 The enzymatic activity of CYP3A4 was estimated by determing the 6beta-hydroxytestosterone metabolized from testosterone performed on a liquid chromatography-tandem mass spectrometry (LC-MS/MS). 6 beta-hydroxytestosterone 64-89 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 26-32 18332045-11 2008 Finally, enhanced 6beta-hydroxytestosterone formation in Dex and phthalate cotreated human hepatocytes confirmed CYP3A4 enzyme induction. 6 beta-hydroxytestosterone 18-43 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 113-119