PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 26144210-2 2015 BraA, this analogue (BraL), and cyclosporine A were tested for their ability to inhibit the proliferation of human T cells upon CD3/CD28 activation. Cyclosporine 32-46 CD28 molecule Homo sapiens 132-136 18606654-5 2008 These signals are dependent on CD28-derived PI3K/Akt pathways and resistant to cyclosporin A. Cyclosporine 79-92 CD28 molecule Homo sapiens 31-35 18617642-8 2008 Cyclosporine A (100 ng/ml) inhibited proliferation of CD4(+)CD28(null) cells by 33 +/- 11% versus 68 +/- 12% inhibition of CD4(+)CD28(+) (P = 0.025). Cyclosporine 0-14 CD28 molecule Homo sapiens 60-64 18617642-8 2008 Cyclosporine A (100 ng/ml) inhibited proliferation of CD4(+)CD28(null) cells by 33 +/- 11% versus 68 +/- 12% inhibition of CD4(+)CD28(+) (P = 0.025). Cyclosporine 0-14 CD28 molecule Homo sapiens 129-133 18845086-6 2008 Between two treatment group, the expression of CD4(+)T cells and the expression of CD28 and ICOS on CD8(+)T cells in CsA-treated group were much higher than those in FK506-treated group (P<0.05), and there was no significant difference between two treatment groups in other indexes. Cyclosporine 117-120 CD28 molecule Homo sapiens 83-87 16044165-5 2005 Computational dissection of these transcriptional profiles in activated T cells uncovers a novel regulatory synergy between IGF-1 and CD28 costimulation that modulates NF-kappaB and AP1 pathways through signaling cascades sensitive to cyclosporin A and wortmannin. Cyclosporine 235-248 CD28 molecule Homo sapiens 134-138 15710339-1 2005 Cyclosporin A (CsA) inhibits interleukin (IL)-2 production, activation and proliferation of human peripheral T cells (HPTC) costimulated with simultaneous engagement of T cell receptor (TCR)/CD3 and CD28. Cyclosporine 15-18 CD28 molecule Homo sapiens 199-203 15710339-2 2005 We demonstrated that 10 ng/ml CsA, which reduced the proliferation of HPTC costimulated with anti-CD3 and anti-CD28 by half, prevented NF-AT and NF-kappaB from migrating into the nucleus. Cyclosporine 30-33 CD28 molecule Homo sapiens 111-115 15710339-4 2005 CsA, which was added to HPTC simultaneously with the engagement of both CD3 and CD28 or the 1-h-delayed engagement of CD28 after prior TCR/CD3-triggering, inhibited NF-kappaB p65/RelA from binding to the target DNA fragment, followed by reduction of HPTC proliferation in response to the costimulation. Cyclosporine 0-3 CD28 molecule Homo sapiens 80-84 15710339-4 2005 CsA, which was added to HPTC simultaneously with the engagement of both CD3 and CD28 or the 1-h-delayed engagement of CD28 after prior TCR/CD3-triggering, inhibited NF-kappaB p65/RelA from binding to the target DNA fragment, followed by reduction of HPTC proliferation in response to the costimulation. Cyclosporine 0-3 CD28 molecule Homo sapiens 118-122 15710339-5 2005 When CsA was added 30 min after the delayed engagement of CD28 following the prior engagement of TCR/CD3, these inhibitory effects were diminished. Cyclosporine 5-8 CD28 molecule Homo sapiens 58-62 15710339-8 2005 These findings indicate that the major effects of CsA on the early phase of CD28-mediated costimulation in the presence of TCR/CD3 signaling are to inhibit NF-kappaB/RelA from translocating into the nucleus and binding to the target DNA sequence in the IL-2 gene promoter region, which induces IL-2 expression leading to HPTC proliferation. Cyclosporine 50-53 CD28 molecule Homo sapiens 76-80 15501456-2 2004 We showed that Curcumin is a potent inhibitor of Cyclosporin A resistant T cell CD28 co-stimulation pathway. Cyclosporine 49-62 CD28 molecule Homo sapiens 80-84 15459204-6 2004 This putative promoter was active in Jurkat T cells following CD3 and CD28 cross-linking, and its activity was inhibited by cyclosporin A and MAPK inhibitors. Cyclosporine 124-137 CD28 molecule Homo sapiens 70-74 10928971-6 2000 Cyclosporin A (CsA) and dexamethasone (DEX) also inhibited the anti-CD3/CD28 induced cytokine production, but were less potent than FK506. Cyclosporine 0-13 CD28 molecule Homo sapiens 72-76 12744774-2 2003 Previous investigations found that costimulation of the CD28 pathway generally mediates CsA-resistant proliferation of T cell receptor (TCR)-activated T lymphocytes. Cyclosporine 88-91 CD28 molecule Homo sapiens 56-60 12744774-3 2003 However, here we describe considerable interindividual variation regarding the immunosuppressive effects of CsA (1000 microg/L) on anti-CD3/CD28 T cell costimulation in a human whole blood assay. Cyclosporine 108-111 CD28 molecule Homo sapiens 140-144 12036883-6 2002 Treatment with rapamycin blocked IL-2 production after activation of human peripheral blood T cells with phorbol ester (PMA) and anti-CD28 (CsA-resistant pathway), whereas this drug did not have any effect on PMA plus ionomycin stimulation (CsA-sensitive pathway). Cyclosporine 140-143 CD28 molecule Homo sapiens 134-138 11981427-8 2002 In addition, a short course of cyclosporine therapy synergized with either anti-CD28 monoclonal antibody or CTLA4 immunoglobulin, suggesting that it may be clinically relevant to combine low-dose calcineurin inhibitors with CTLA4 immunoglobulin or anti-B7 antibodies. Cyclosporine 31-43 CD28 molecule Homo sapiens 80-84 11357886-4 2001 Cyclosporin A (CsA) also inhibited the anti-CD3/CD28 induced IL-6 production but was about 100 times less potent than FK506. Cyclosporine 0-13 CD28 molecule Homo sapiens 48-52 11357886-4 2001 Cyclosporin A (CsA) also inhibited the anti-CD3/CD28 induced IL-6 production but was about 100 times less potent than FK506. Cyclosporine 15-18 CD28 molecule Homo sapiens 48-52 14709642-10 2004 Two individuals responded conversely, indicating that differences in the in vitro response to tacrolimus and CsA among individuals may be attributable to potential heterogeneity in the involvement of the CD28 pathway. Cyclosporine 109-112 CD28 molecule Homo sapiens 204-208 12097397-4 2002 Cross-linking of neutrophil-expressed CD28 by monoclonal anti-CD28 Ab or B7.1-Ig or B7.2-Ig results in phosphatidylinositol 3-kinase association with CD28 and in wortmannin-sensitive but cyclosporin A-resistant induction and secretion of IFN-gamma. Cyclosporine 187-200 CD28 molecule Homo sapiens 38-42 12097397-4 2002 Cross-linking of neutrophil-expressed CD28 by monoclonal anti-CD28 Ab or B7.1-Ig or B7.2-Ig results in phosphatidylinositol 3-kinase association with CD28 and in wortmannin-sensitive but cyclosporin A-resistant induction and secretion of IFN-gamma. Cyclosporine 187-200 CD28 molecule Homo sapiens 62-66 12097397-4 2002 Cross-linking of neutrophil-expressed CD28 by monoclonal anti-CD28 Ab or B7.1-Ig or B7.2-Ig results in phosphatidylinositol 3-kinase association with CD28 and in wortmannin-sensitive but cyclosporin A-resistant induction and secretion of IFN-gamma. Cyclosporine 187-200 CD28 molecule Homo sapiens 62-66 12036883-0 2002 Effect of rapamycin on the cyclosporin A-resistant CD28-mediated costimulatory pathway. Cyclosporine 27-40 CD28 molecule Homo sapiens 51-55 12036883-4 2002 The signaling event mediated by CD28 engagement has been proposed to have 2 components: one is sensitive to the immunosuppressive drug cyclosporin A (CsA), and the other one is CsA-resistant. Cyclosporine 135-148 CD28 molecule Homo sapiens 32-36 12036883-4 2002 The signaling event mediated by CD28 engagement has been proposed to have 2 components: one is sensitive to the immunosuppressive drug cyclosporin A (CsA), and the other one is CsA-resistant. Cyclosporine 150-153 CD28 molecule Homo sapiens 32-36 12036883-4 2002 The signaling event mediated by CD28 engagement has been proposed to have 2 components: one is sensitive to the immunosuppressive drug cyclosporin A (CsA), and the other one is CsA-resistant. Cyclosporine 177-180 CD28 molecule Homo sapiens 32-36 21336903-6 2001 CD28 ligation provides cyclosporin A-resistant biochemical signals to T cells, which are an absolute requirement to drive proliferation and IL-2 production from CD3-stimulated T cells, as well as enhance cell survival (5,6). Cyclosporine 23-36 CD28 molecule Homo sapiens 0-4 11093160-9 2000 In untransformed human peripheral blood T lymphocytes, these phosphatases function through a cyclosporin A/FK506-resistant co-stimulatory signaling pathway which is common for the accessory receptors CD2 and CD28. Cyclosporine 93-106 CD28 molecule Homo sapiens 208-212 10928971-6 2000 Cyclosporin A (CsA) and dexamethasone (DEX) also inhibited the anti-CD3/CD28 induced cytokine production, but were less potent than FK506. Cyclosporine 15-18 CD28 molecule Homo sapiens 72-76 10928971-7 2000 FK506 and CsA, but not DEX, specifically inhibited anti-CD3/CD28 induced inflammatory cytokine production without affecting the lipopolysaccaride (LPS) induced effect. Cyclosporine 10-13 CD28 molecule Homo sapiens 60-64 10760803-7 2000 This is further suggested by the demonstration that LFA-1 and CD28 acted synergistically to confer CsA resistance in a model of co-stimulation using superantigen-pulsed dendritic cells. Cyclosporine 99-102 CD28 molecule Homo sapiens 62-66 10202558-2 1999 The best characterized costimulatory receptor expressed on resting T cells is CD28 which provides poorly-defined cyclosporin-resistant biochemical signal(s) that promote expression of several cytokines/chemokines. Cyclosporine 113-124 CD28 molecule Homo sapiens 78-82 10589954-2 1999 Previous studies have shown that stimulation of T cells via CD28 or phorbol myristate acetate (PMA) activation is highly resistant to inhibition by cyclosporine A (CsA) and tacrolimus (FK506), as is the response of T cells to phytohemmaglutinin in the presence of endothelial cells. Cyclosporine 148-162 CD28 molecule Homo sapiens 60-64 10589954-2 1999 Previous studies have shown that stimulation of T cells via CD28 or phorbol myristate acetate (PMA) activation is highly resistant to inhibition by cyclosporine A (CsA) and tacrolimus (FK506), as is the response of T cells to phytohemmaglutinin in the presence of endothelial cells. Cyclosporine 164-167 CD28 molecule Homo sapiens 60-64 9603452-8 1998 In conclusion, IFN-gamma production by CD28-co-stimulated CD4+ T cells is resistant to inhibition by CsA and can even be facilitated by CsA as a result of removing a negative regulatory signal which is mainly IL-10 mediated. Cyclosporine 101-104 CD28 molecule Homo sapiens 39-43 9733605-0 1998 Curcumin blocks cyclosporine A-resistant CD28 costimulatory pathway of human T-cell proliferation. Cyclosporine 16-30 CD28 molecule Homo sapiens 41-45 9603452-8 1998 In conclusion, IFN-gamma production by CD28-co-stimulated CD4+ T cells is resistant to inhibition by CsA and can even be facilitated by CsA as a result of removing a negative regulatory signal which is mainly IL-10 mediated. Cyclosporine 136-139 CD28 molecule Homo sapiens 39-43 7516408-5 1994 The proliferative effect of anti-CD28 and IL-12 is resistant to moderate doses of cyclosporin A and is largely independent of endogenous IL-2, IL-12, in synergy with anti-CD28 or B7-transfected cells, is most effective in inducing interferon gamma (IFN-gamma) production, but production of tumor necrosis factor alpha and granulocyte/macrophage colony-stimulating factor is also observed. Cyclosporine 82-95 CD28 molecule Homo sapiens 33-37 9640246-6 1998 T-cell proliferation mediated through CD6/CD28 was only partially blocked by the immunosuppressive drug, cyclosporin A (CsA), whereas anti-CD28-induced T-cell proliferation in the presence of the phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), was unaffected. Cyclosporine 105-118 CD28 molecule Homo sapiens 42-46 9640246-6 1998 T-cell proliferation mediated through CD6/CD28 was only partially blocked by the immunosuppressive drug, cyclosporin A (CsA), whereas anti-CD28-induced T-cell proliferation in the presence of the phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), was unaffected. Cyclosporine 120-123 CD28 molecule Homo sapiens 42-46 9548474-6 1997 In contrast, cross-linking of CD47 in the presence of CD28 mAb or phorbol ester induces vigorous T cell proliferation that is sensitive to cyclosporin A. Cyclosporine 139-152 CD28 molecule Homo sapiens 54-58 8809021-3 1996 The biochemical signals provided by CD28 are cyclosporin A-resistant and complement those provided by the T cell antigen receptor to allow full activation of T cells. Cyclosporine 45-56 CD28 molecule Homo sapiens 36-40 8757955-4 1996 The CsA-resistant component is completely suppressed either by blocking with anti-CD28 F(ab) fragments or CTLA-4-Ig. Cyclosporine 4-7 CD28 molecule Homo sapiens 82-86 8757955-8 1996 These findings demonstrate that primary unstimulated porcine EC can co-stimulate CsA-resistant human T cell proliferation through binding of membrane bound, constitutively expressed EC B7-2 (CD86) to human T cell CD28, providing one of the first demonstrations of functional B7-2 on cells outside the immune system. Cyclosporine 81-84 CD28 molecule Homo sapiens 213-217 8631809-4 1996 We demonstrate here, however, that NFAT can be activated, and significant levels of IL-2 can be produced by the CsA-resistant CD28-signaling pathway. Cyclosporine 112-115 CD28 molecule Homo sapiens 126-130 8631809-5 1996 In transient transfection assays, both multicopy NFAT- and IL-2 promoter-beta-galactosidase reporter gene constructs could be activated by phorbol 12-myristate 13-acetate (PMA)/alpha-CD28 stimulation, and this activation was resistant to CsA. Cyclosporine 238-241 CD28 molecule Homo sapiens 183-187 7634349-8 1995 Finally, both the CD3 plus CD27 and the CD28 plus CD27-stimulated T cell proliferation is sensitive to inhibition by CsA. Cyclosporine 117-120 CD28 molecule Homo sapiens 40-44 7534790-13 1995 We conclude that CD28 costimulatory signals augment superantigen-induced TCR signals by converging onto common TCR effector pathways involving the activation of phospholipase C gamma 1 and PKC and by generating a cyclosporin A-sensitive pathway. Cyclosporine 213-226 CD28 molecule Homo sapiens 17-21 7957566-12 1994 These data provide a model for CD28 signal transduction and support a role for PI-3 kinase in mediating the CD28 calcium-independent, cyclosporin A-insensitive costimulatory signal. Cyclosporine 134-147 CD28 molecule Homo sapiens 108-112 8596025-7 1996 Finally, cyclosporin A blocked both IL-2 secretion and bcl-2 induction in response to CD3 plus CD28 stimulation, suggesting a role for endogenous lymphokine production in the induction of bcl-2. Cyclosporine 9-22 CD28 molecule Homo sapiens 95-99 7982907-2 1994 CD28 signaling is resistant to the immunosuppressant cyclosporin A (CsA) but sensitive to the immunosuppressant rapamycin. Cyclosporine 53-66 CD28 molecule Homo sapiens 0-4 7982907-2 1994 CD28 signaling is resistant to the immunosuppressant cyclosporin A (CsA) but sensitive to the immunosuppressant rapamycin. Cyclosporine 68-71 CD28 molecule Homo sapiens 0-4 7524221-0 1994 Individual susceptibility to cyclosporine: possible involvement of the CD28/CD80 (B7/BB1) pathway. Cyclosporine 29-41 CD28 molecule Homo sapiens 71-75 8077662-5 1994 Furthermore, cyclosporin A, but not rapamycin, blocked the synergistic induction of IL-8 expression achieved with anti-CD3 and anti-CD28 costimulation. Cyclosporine 13-26 CD28 molecule Homo sapiens 132-136 7878594-4 1994 To explain these differences, the CD28-B7, co-stimulatory pathway for T-cell activation was explored since it is the only CsA-resistant pathway known so far. Cyclosporine 122-125 CD28 molecule Homo sapiens 34-38 7515910-7 1994 Both CD40L expression and T cell help were blocked by cyclosporin A after TCR cross-linking, and, unlike T cell proliferation, both remained cyclosporin A sensitive during CD28 costimulation. Cyclosporine 141-154 CD28 molecule Homo sapiens 172-176 8183372-3 1994 The signalling pathways used by CD28 are unlike those used by the CD3/T-cell receptor in that they are resistant to cyclosporin A and independent of changes in cyclic AMP concentrations. Cyclosporine 116-129 CD28 molecule Homo sapiens 32-36 7506079-7 1994 However, low CsA concentrations, when combined with an agent blocking B7-CD28 interaction, can potentially achieve complete immunosuppression. Cyclosporine 13-16 CD28 molecule Homo sapiens 73-77 7507511-1 1994 Interaction of CD28/CTLA-4 on T cells with B7 on antigen-presenting cells constitutes an important costimulatory signal for T cells and is responsible for cyclosporin A-resistant interleukin 2 (IL-2) gene expression and potentially also for prevention of anergy induction after T cell receptor triggering. Cyclosporine 155-168 CD28 molecule Homo sapiens 15-19 8302298-11 1994 The immunosuppressive drugs Cyclosporin A and FK506 completely blocked CD28 and CD3 mediated IL-2 production in these transfectants whereas rapamycin had only a partial inhibitory effect. Cyclosporine 28-41 CD28 molecule Homo sapiens 71-75 7682237-0 1993 CD28 ligation by monoclonal antibodies or B7/BB1 provides an accessory signal for the cyclosporin A-resistant generation of cytotoxic T cell activity. Cyclosporine 86-99 CD28 molecule Homo sapiens 0-4 7682237-10 1993 We conclude that CD28 ligation provides a major accessory signal for the CsA-resistant generation of CTL activity and that CD28-B7 interaction also enhances cytotoxic effector functions of CTL. Cyclosporine 73-76 CD28 molecule Homo sapiens 17-21 1348520-0 1992 CD28-stimulated IL-2 gene expression in Jurkat T cells occurs in part transcriptionally and is cyclosporine-A sensitive. Cyclosporine 95-109 CD28 molecule Homo sapiens 0-4 1348520-2 1992 Previous studies have shown that proliferation of peripheral blood T cells involving the CD28 pathway is associated with cyclosporine A (CsA) resistant IL-2 gene expression. Cyclosporine 121-135 CD28 molecule Homo sapiens 89-93 1847252-1 1991 In the present investigation, we compared the immunosuppressive effects of genistein and CsA on anti-CD28 stimulated human T cell proliferation, IL-2 production, and IL-2R expression. Cyclosporine 89-92 CD28 molecule Homo sapiens 101-105 1705509-10 1991 This contrasts with anti-CD28/PMA-induced T cell proliferation, which is resistant to CsA and PGE2. Cyclosporine 86-89 CD28 molecule Homo sapiens 25-29 1646506-0 1991 The effect of the CD28 activation pathway on the immunosuppressive action of cyclosporine. Cyclosporine 77-89 CD28 molecule Homo sapiens 18-22 1646506-1 1991 The effect of the CD28 activation pathway on the immunosuppressive action of CsA was assessed. Cyclosporine 77-80 CD28 molecule Homo sapiens 18-22 1646506-10 1991 In comparison, the proliferative response and IL-2 production elicited by anti-CD3 + anti-CD28 was more resistant to the effects of CsA (IC50 = 100-200 nM). Cyclosporine 132-135 CD28 molecule Homo sapiens 90-94 1646506-12 1991 Combination drug:drug studies revealed that CsA and the protein kinase C inhibitor H-7 were additive for both anti-CD3 and anti-CD3 plus anti-CD28 response. Cyclosporine 44-47 CD28 molecule Homo sapiens 142-146 1646506-13 1991 On the other hand, the cGMP-dependent protein kinase inhibitor H-8 was synergistic with CsA in inhibiting the response of lymphocytes to anti-CD3 plus anti-CD28 but only additive for responses to anti-CD3. Cyclosporine 88-91 CD28 molecule Homo sapiens 156-160 1646506-15 1991 Activation of the CD28 pathway partially overcomes the inhibitory activity of CsA on IL-2 production and may be mediated by indirect activation of a cGMP-dependent protein kinase. Cyclosporine 78-81 CD28 molecule Homo sapiens 18-22 1847252-3 1991 In contrast, proliferation of T cells stimulated with PMA plus anti-CD28 is resistant to the inhibitory effects of CsA. Cyclosporine 115-118 CD28 molecule Homo sapiens 68-72 1847252-5 1991 PHA plus anti-CD28 or PMA plus anti-CD28-induced IL-2 synthesis was inhibited by genistein, and CsA, though it inhibited the PHA plus PMA-stimulated IL-2 synthesis, failed to have any effect on PMA plus anti-CD28-induced IL-2 synthesis. Cyclosporine 96-99 CD28 molecule Homo sapiens 14-18 1847252-5 1991 PHA plus anti-CD28 or PMA plus anti-CD28-induced IL-2 synthesis was inhibited by genistein, and CsA, though it inhibited the PHA plus PMA-stimulated IL-2 synthesis, failed to have any effect on PMA plus anti-CD28-induced IL-2 synthesis. Cyclosporine 96-99 CD28 molecule Homo sapiens 36-40 1847252-5 1991 PHA plus anti-CD28 or PMA plus anti-CD28-induced IL-2 synthesis was inhibited by genistein, and CsA, though it inhibited the PHA plus PMA-stimulated IL-2 synthesis, failed to have any effect on PMA plus anti-CD28-induced IL-2 synthesis. Cyclosporine 96-99 CD28 molecule Homo sapiens 36-40 1676547-8 1991 The response via CD2 plus CD28 is IL-2-dependent, as demonstrated by the ability of mAb against the IL-2 receptor to block proliferation, and is almost completely inhibited by cyclosporine A (CsA). Cyclosporine 192-195 CD28 molecule Homo sapiens 26-30 1676547-8 1991 The response via CD2 plus CD28 is IL-2-dependent, as demonstrated by the ability of mAb against the IL-2 receptor to block proliferation, and is almost completely inhibited by cyclosporine A (CsA). Cyclosporine 176-190 CD28 molecule Homo sapiens 26-30 35553638-10 2022 Cyclosporine A and prednisolone synergistically increased GCR expression and inhibited pro-inflammatory cytokine production by CD28null CD8- T and NKT-like cells. Cyclosporine 0-14 CD28 molecule Homo sapiens 127-131 2465550-5 1989 The activation pathway induced by stimulation of CD28 is distinct from other biochemical pathways that induce lymphokines/cytokines because CD28 stimulation can induce lymphokine/cytokine gene expression in the presence of the immunosuppressant cyclosporine. Cyclosporine 245-257 CD28 molecule Homo sapiens 49-53 2465550-5 1989 The activation pathway induced by stimulation of CD28 is distinct from other biochemical pathways that induce lymphokines/cytokines because CD28 stimulation can induce lymphokine/cytokine gene expression in the presence of the immunosuppressant cyclosporine. Cyclosporine 245-257 CD28 molecule Homo sapiens 140-144 2830495-0 1987 T-cell proliferation involving the CD28 pathway is associated with cyclosporine-resistant interleukin 2 gene expression. Cyclosporine 67-79 CD28 molecule Homo sapiens 35-39 2830495-9 1987 Functional studies revealed that the proliferation induced by CD28 and PMA stimulation was entirely resistant to cyclosporine, in contrast to T-cell activation induced by the CD3-T-cell receptor complex. Cyclosporine 113-125 CD28 molecule Homo sapiens 62-66 2830495-11 1987 Furthermore, stimulation by CD28 in combination with immobilized CD3 antibodies caused a striking enhancement of IL-2 mRNA expression that was, in part, resistant to the effects of cyclosporine. Cyclosporine 181-193 CD28 molecule Homo sapiens 28-32 33317956-9 2021 Treatment with prednisolone + theophylline + cyclosporin A inhibited IFN-gamma and TNF-alpha production by SA CD28null CD8+ T and NKT-like cells additively. Cyclosporine 45-58 CD28 molecule Homo sapiens 110-114 2543699-2 1989 Prior results indicated that stimulation of T cells by anti-CD28 mAb plus PMA could induce IL-2 expression and T cell proliferation that was entirely resistant to cyclosporine. Cyclosporine 163-175 CD28 molecule Homo sapiens 60-64 2543699-6 1989 The addition of anti-CD28 to T cells stimulated with PMA plus calcium ionophore induced a 5- to 100-fold increase in IL-2 gene expression and secretion that was resistant to cyclosporine. Cyclosporine 174-186 CD28 molecule Homo sapiens 21-25 2543699-9 1989 The signal provided by CD28 is distinct from that of CD3 because although anti-CD28 plus PMA-induced proliferation is resistant to cyclosporine, anti-CD3 or anti-CD3 plus PMA-induced IL-2 expression is sensitive. Cyclosporine 131-143 CD28 molecule Homo sapiens 23-27 2567675-5 1989 CyA-induced inhibition of both anti-CD3- and anti-CD2-mediated proliferation could not be reversed by addition of either PMA (1 ng/ml) or anti-CD28. Cyclosporine 0-3 CD28 molecule Homo sapiens 143-147 2536062-6 1989 Whereas cyclosporin A did not inhibit the effect of PMA, it reduced the effects of agonists to TCR/CD3 and CD28 on the LTR. Cyclosporine 8-21 CD28 molecule Homo sapiens 107-111 2536062-8 1989 Thus, PMA activates the NF kappa B sites through a PKC-dependent pathway while ligands to TCR/CD3 and CD28 activate the LTR through a cyclosporin A-sensitive, PKC-dependent pathway of T cell activation. Cyclosporine 134-147 CD28 molecule Homo sapiens 102-106 2830495-12 1987 These studies indicate that the CD28 molecule synergizes with protein kinase C activation to induce IL-2 gene expression and demonstrate that stimulation by the CD28 pathway can cause vigorous T-cell proliferation even in the presence of cyclosporine and that cyclosporine does not prevent transcription of 16-2 mRNA, as has been suggested previously. Cyclosporine 238-250 CD28 molecule Homo sapiens 32-36 2830495-12 1987 These studies indicate that the CD28 molecule synergizes with protein kinase C activation to induce IL-2 gene expression and demonstrate that stimulation by the CD28 pathway can cause vigorous T-cell proliferation even in the presence of cyclosporine and that cyclosporine does not prevent transcription of 16-2 mRNA, as has been suggested previously. Cyclosporine 238-250 CD28 molecule Homo sapiens 161-165 2830495-12 1987 These studies indicate that the CD28 molecule synergizes with protein kinase C activation to induce IL-2 gene expression and demonstrate that stimulation by the CD28 pathway can cause vigorous T-cell proliferation even in the presence of cyclosporine and that cyclosporine does not prevent transcription of 16-2 mRNA, as has been suggested previously. Cyclosporine 260-272 CD28 molecule Homo sapiens 32-36 2830495-12 1987 These studies indicate that the CD28 molecule synergizes with protein kinase C activation to induce IL-2 gene expression and demonstrate that stimulation by the CD28 pathway can cause vigorous T-cell proliferation even in the presence of cyclosporine and that cyclosporine does not prevent transcription of 16-2 mRNA, as has been suggested previously. Cyclosporine 260-272 CD28 molecule Homo sapiens 161-165