PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11739185-5 2001 Fas signaling can be interrupted at 3 mains levels: Fas clustering, alteration of death-inducing-signaling-complex (DISC) formation, and effector caspase inhibition of downstream caspase-8. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 179-188 11739185-7 2001 However, pretreatment with chelerythrin, but not with calphostin C, resulted in the restoration of Fas-induced caspase-8 activation and cytotoxicity, suggesting that some atypical protein kinase C (PKC) isoforms contributed to the lack of DISC formation. ammonium ferrous sulfate 99-102 caspase 8 Homo sapiens 111-120 11673515-1 2001 Previous studies of thymocyte apoptosis using a series of cell-permeable fluorogenic peptide substrates showed that Fas cross-linking triggered a caspase cascade in which cleavage of the IETDase (caspase 8-selective) substrate was the earliest caspase activity measured by flow cytometry. ammonium ferrous sulfate 116-119 caspase 8 Homo sapiens 196-205 11737081-11 2001 These agents also reduced the period of resistance to Fas-mediated apoptosis after T cell activation, possibly by reducing expression of c-FLIP, allowing early activation of caspase 8 in alloreactive T cells. ammonium ferrous sulfate 54-57 caspase 8 Homo sapiens 174-183 11925935-5 2001 In the present study, we demonstrate that caspase-8 is cleaved in control transfectant KB cells early on during Fas-induced apoptosis. ammonium ferrous sulfate 112-115 caspase 8 Homo sapiens 42-51 11925935-7 2001 In contrast, Fas-induced caspase-8 cleavage is inhibited in KB/TP cells, which lead to inhibition of the downstream apoptotic cascade and inhibition of apoptosis. ammonium ferrous sulfate 13-16 caspase 8 Homo sapiens 25-34 11581255-7 2001 Here we report that PKC alters Fas/CD95 signaling from the plasma membrane to the activation of caspases by exerting a profound action on survival/cell death decisions. ammonium ferrous sulfate 31-34 caspase 8 Homo sapiens 96-104 11698497-6 2001 We conclude that caspase-8 and caspase-3 activation, but not MPTP opening, mediate Fas-induced eosinophil apoptosis and are the main targets for the protective effect of IL-5 and IFN-gamma. ammonium ferrous sulfate 83-86 caspase 8 Homo sapiens 17-26 11275978-4 2001 Our data indicated that the resistance of 2 selected Fas-transfected clones to CD95-mediated lysis correlated with down-regulation of caspase-8 or its lack of cleavage and subsequent activation. ammonium ferrous sulfate 53-56 caspase 8 Homo sapiens 134-143 11765224-0 2001 Expression of Fas-associated death domain-like interleukin-1beta-converting enzyme (FLICE) inhibitory protein (FLIP) in human articular chondrocytes: possible contribution to the resistance to Fas-mediated death of in vitro cultured human articular chondrocytes. ammonium ferrous sulfate 14-17 caspase 8 Homo sapiens 84-89 11427719-2 2001 vICA inhibits Fas-mediated apoptosis by binding to the pro-domain of caspase-8 and preventing its activation. ammonium ferrous sulfate 14-17 caspase 8 Homo sapiens 69-78 11439165-9 2001 When studying the activity of some of these isoforms, including FLIPR, they all efficiently inhibited Fas-mediated apoptosis in A20 B lymphoma cells by impeding caspase-8, -3 and -7 activity as well as poly(ADP-ribose) polymerase (PARP) cleavage. ammonium ferrous sulfate 102-105 caspase 8 Homo sapiens 161-181 11439335-8 2001 A Fas-antagonistic mAb (ZB-4) inhibited the Fas-agonistic mAb CH-11-induced caspase-8 activation and apoptosis in Jurkat T-leukemic cells (used as positive control), but had no effect on mAb 225-induced activation of caspase-8 and apoptosis in DiFi cells. ammonium ferrous sulfate 2-5 caspase 8 Homo sapiens 76-85 11439335-8 2001 A Fas-antagonistic mAb (ZB-4) inhibited the Fas-agonistic mAb CH-11-induced caspase-8 activation and apoptosis in Jurkat T-leukemic cells (used as positive control), but had no effect on mAb 225-induced activation of caspase-8 and apoptosis in DiFi cells. ammonium ferrous sulfate 2-5 caspase 8 Homo sapiens 217-226 11439335-8 2001 A Fas-antagonistic mAb (ZB-4) inhibited the Fas-agonistic mAb CH-11-induced caspase-8 activation and apoptosis in Jurkat T-leukemic cells (used as positive control), but had no effect on mAb 225-induced activation of caspase-8 and apoptosis in DiFi cells. ammonium ferrous sulfate 44-47 caspase 8 Homo sapiens 76-85 11439335-8 2001 A Fas-antagonistic mAb (ZB-4) inhibited the Fas-agonistic mAb CH-11-induced caspase-8 activation and apoptosis in Jurkat T-leukemic cells (used as positive control), but had no effect on mAb 225-induced activation of caspase-8 and apoptosis in DiFi cells. ammonium ferrous sulfate 44-47 caspase 8 Homo sapiens 217-226 11599886-0 2001 Fas-independent apoptosis induced by UVC in p53-mutated human epithelial tumor A431 cells through activation of caspase-8 and JNK/SAPK. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 112-121 11375056-8 2001 In these subjects the Fas-related caspase 8 (FLICE) was significantly increased in cells treated with the recombinant Fas. ammonium ferrous sulfate 22-25 caspase 8 Homo sapiens 45-50 11375056-8 2001 In these subjects the Fas-related caspase 8 (FLICE) was significantly increased in cells treated with the recombinant Fas. ammonium ferrous sulfate 118-121 caspase 8 Homo sapiens 45-50 11431480-7 2001 The results show that the activation of caspase-8, but not caspase-9, is necessary for Fas-induced apoptosis. ammonium ferrous sulfate 87-90 caspase 8 Homo sapiens 40-49 11525634-5 2001 However, while Fas-dependent apoptosis absolutely required caspase-8 activity, it is not necessary for completion of the apoptotic program induced by IR and MMC. ammonium ferrous sulfate 15-18 caspase 8 Homo sapiens 59-68 11406564-7 2001 In vitro down-regulation of bcl-2 with antisense oligonucleotides allowed the release of cytochrome c from mitochondria and the activation of caspases 8 and 3 upon Fas activation as well as sensitized neuroblastoma cells to Fas-mediated apoptosis. ammonium ferrous sulfate 164-167 caspase 8 Homo sapiens 142-158 11406564-10 2001 These data indicate that Fas-mediated apoptosis in neuroblastoma cells is mitochondria-dependent and inhibited both at the mitochondrial level and at the level of caspase 8 activation. ammonium ferrous sulfate 25-28 caspase 8 Homo sapiens 163-172 11238127-10 2001 These results suggest that the long isoform of caspase-2 plays a role in the Fas-mediated pathway to cell death by contributing to caspase-8 activation at the DISC level. ammonium ferrous sulfate 77-80 caspase 8 Homo sapiens 131-140 11238216-0 2001 Butyric acid-induced T-cell apoptosis is mediated by caspase-8 and -9 activation in a Fas-independent manner. ammonium ferrous sulfate 86-89 caspase 8 Homo sapiens 53-69 11238216-8 2001 Time-dependent activation of caspase-8 and -9 was recognized in butyric acid- as well as Fas-mediated apoptosis. ammonium ferrous sulfate 89-92 caspase 8 Homo sapiens 29-45 11238216-9 2001 IETD-CHO and LEHD-CHO, specific inhibitors of caspase-8 and -9, respectively, completely blocked Fas-mediated apoptosis and partially prevented butyric acid-induced apoptosis. ammonium ferrous sulfate 97-100 caspase 8 Homo sapiens 46-62 11157988-5 2001 Matrix attachment modulates Fas-mediated apoptosis at two different levels: by regulating the expression level of Fas, and by regulating the expression level of c-Flip, an endogenous antagonist of caspase-8. ammonium ferrous sulfate 28-31 caspase 8 Homo sapiens 197-206 11063743-4 2001 The Fas pathway was blocked at the level of caspase-8, whereas the intrinsic pathway was blocked at the mitochondria. ammonium ferrous sulfate 4-7 caspase 8 Homo sapiens 44-53 11110692-9 2000 Therefore fas-induced apoptosis in Fanconi anemia cells of the C type involves the activation of caspase 8, which controls activation of caspase 3 family members and one direct or indirect function of the FANCC protein is to suppress apoptotic responses to IFN-gamma upstream of caspase 3 activation. ammonium ferrous sulfate 10-13 caspase 8 Homo sapiens 97-106 11221844-8 2001 Caspase-8-deficient Jurkat cells were resistant to both TRAIL and Fas-induced apoptosis, whereas wild-type Jurkat cells were susceptible to both TRAIL- and Fas-induced apoptosis. ammonium ferrous sulfate 66-69 caspase 8 Homo sapiens 0-9 11221844-8 2001 Caspase-8-deficient Jurkat cells were resistant to both TRAIL and Fas-induced apoptosis, whereas wild-type Jurkat cells were susceptible to both TRAIL- and Fas-induced apoptosis. ammonium ferrous sulfate 156-159 caspase 8 Homo sapiens 0-9 11180403-4 2001 In addition, the inhibition assay revealed that caspase-8 is located upstream of both caspase-3 and -7, suggesting that Stx1-mediated apoptosis utilizes a similar caspase cascade to that involved in Fas-mediated apoptosis. ammonium ferrous sulfate 199-202 caspase 8 Homo sapiens 48-57 11180403-4 2001 In addition, the inhibition assay revealed that caspase-8 is located upstream of both caspase-3 and -7, suggesting that Stx1-mediated apoptosis utilizes a similar caspase cascade to that involved in Fas-mediated apoptosis. ammonium ferrous sulfate 199-202 caspase 8 Homo sapiens 48-55 10960761-5 2000 Activation of caspase-8 in response to Fas triggering by recruitment of caspase-8 to the Fas has also been found, however, MMC did not induce FasL and Fas expression, as evidenced by reverse transcriptase-polymerase chain reaction and Western blotting. ammonium ferrous sulfate 39-42 caspase 8 Homo sapiens 14-23 11101870-0 2000 Fas triggers an alternative, caspase-8-independent cell death pathway using the kinase RIP as effector molecule. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 29-38 11010806-6 2000 Fas-mediated apoptosis of senescent fibroblasts was evidenced by chromosome condensation and by activation of caspase-8 and -3, proteases crucial for the execution of the Fas apoptosis pathway. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 110-126 10960761-5 2000 Activation of caspase-8 in response to Fas triggering by recruitment of caspase-8 to the Fas has also been found, however, MMC did not induce FasL and Fas expression, as evidenced by reverse transcriptase-polymerase chain reaction and Western blotting. ammonium ferrous sulfate 39-42 caspase 8 Homo sapiens 72-81 11004695-1 2000 Lymphoma cells often display in vitro resistance to FAS-induced apoptosis, in which caspases act as crucial cell death effectors. ammonium ferrous sulfate 52-55 caspase 8 Homo sapiens 84-92 11004695-3 2000 To investigate the mechanism of FAS resistance, the expression of caspase-8 was analysed by immunohistochemistry, together with that of the substrates caspase-3 and ICAD, in 52 representative samples from non Hodgkin"s lymphoma (NHL), 12 from Hodgkin"s disease (HD), and eight benign lymphoid tissues. ammonium ferrous sulfate 32-35 caspase 8 Homo sapiens 66-75 10964557-1 2000 Caspase-8 is an initiator enzyme in the Fas-mediated pathway of which the downstream executioner caspase-3 is a physiological target. ammonium ferrous sulfate 40-43 caspase 8 Homo sapiens 0-9 10964568-8 2000 The properties of the FADD-DD structure are discussed with respect to previously reported mutagenesis data and emerging models for FasL-induced FADD recruitment to Fas and caspase-8 activation. ammonium ferrous sulfate 131-134 caspase 8 Homo sapiens 172-181 11042678-4 2000 Furthermore, a caspase-8-negative, Fas-resistant Jurkat cell line was sensitive to resveratrol-induced apoptosis which could be strongly inhibited in the Jurkat as well as in the CEM cell line by z-VAD-fmk and z-IETD-fmk. ammonium ferrous sulfate 35-38 caspase 8 Homo sapiens 15-24 10744730-8 2000 In addition Mad1 interfered with Fas-, TRAIL-, and UV-induced apoptosis, which coincided with a reduced activation of caspase-8 during Fas-mediated apoptosis in response to Mad1 expression. ammonium ferrous sulfate 33-36 caspase 8 Homo sapiens 118-127 10801880-2 2000 In Fas-induced apoptosis, Bid is activated through cleavage by caspase 8 into a 15.5-kDa C-terminal fragment (t(c)Bid) and a 6.5 kDa N-terminal fragment (t(n)Bid). ammonium ferrous sulfate 3-6 caspase 8 Homo sapiens 63-72 10799503-0 2000 The prodomain of caspase-1 enhances Fas-mediated apoptosis through facilitation of caspase-8 activation. ammonium ferrous sulfate 36-39 caspase 8 Homo sapiens 83-92 10799503-5 2000 This enhancement of Fas-mediated apoptosis was abolished by inhibitors of caspase-8 (Ile-Glu-Thr-Asp-fluoromethyl ketone) and caspase-3 (Asp-Glu-Val-Asp-aldehyde) but was only slightly diminished by an inhibitor of caspase-1 (acetyl-Tyr-Val-Ala-Asp-chloromethyl ketone). ammonium ferrous sulfate 20-23 caspase 8 Homo sapiens 74-83 10958671-3 2000 To examine the consequences of maintaining the structural integrity of BAP31 during apoptosis, the caspase recognition aspartate residues were mutated to alanine residues, and Fas-mediated activation of caspase 8 and cell death were examined in human KB epithelial cells stably expressing the caspase-resistant mutant crBAP31. ammonium ferrous sulfate 176-179 caspase 8 Homo sapiens 203-212 10871865-6 2000 The first pathway is utilized by lymphoid cells stimulated through Fas, is abrogated in a caspase-8-deficient T-cell line, and is blocked by the caspase inhibitors Z-VAD-fmk and Boc-D-fmk. ammonium ferrous sulfate 67-70 caspase 8 Homo sapiens 90-99 10871865-6 2000 The first pathway is utilized by lymphoid cells stimulated through Fas, is abrogated in a caspase-8-deficient T-cell line, and is blocked by the caspase inhibitors Z-VAD-fmk and Boc-D-fmk. ammonium ferrous sulfate 67-70 caspase 8 Homo sapiens 90-97 10972087-10 2000 By exploring the Fas pathway in Jurkat cells, we found that both apoptosis inducers acted through Fas, since Fas-L, as well as CPP32 and FLICE were activated. ammonium ferrous sulfate 17-20 caspase 8 Homo sapiens 137-142 10863977-5 2000 Moreover, LC induced the activation of caspase 8 (FLICE) protease, which is the initiator of the Fas-mediated apoptotic cascade. ammonium ferrous sulfate 97-100 caspase 8 Homo sapiens 39-48 10863977-5 2000 Moreover, LC induced the activation of caspase 8 (FLICE) protease, which is the initiator of the Fas-mediated apoptotic cascade. ammonium ferrous sulfate 97-100 caspase 8 Homo sapiens 50-55 10852976-6 2000 Interestingly, Fas ligation activated caspase-8 and caspase-3 with the cleavage of poly(ADP-ribose) polymerase (PARP), corresponding to apoptosis of RA synoviocytes. ammonium ferrous sulfate 15-18 caspase 8 Homo sapiens 38-47 10852976-7 2000 Furthermore, specific inhibitors for caspase-3 and caspase-8 but not caspase-1 suppressed Fas-induced apoptosis of RA synoviocytes in a dose- and time-dependent manner. ammonium ferrous sulfate 90-93 caspase 8 Homo sapiens 51-60 10852976-10 2000 CONCLUSION: Our findings suggest that Fas-mediated apoptosis in synoviocytes may be regulated at the level of recruitment of FADD to the DISC, subsequently leading to the activation of the FADD/caspase-8/caspase-3 signalling pathway. ammonium ferrous sulfate 38-41 caspase 8 Homo sapiens 194-203 10480431-6 1999 Coculture with increasing amounts of DC prevented CD95/Fas-triggered apoptosis in a dose-dependent fashion by inhibiting activation of caspase 8 and caspase 3. ammonium ferrous sulfate 55-58 caspase 8 Homo sapiens 135-144 10535957-3 1999 vMIA, a product of the viral UL37 gene, inhibits Fas-mediated apoptosis at a point downstream of caspase-8 activation and Bid cleavage but upstream of cytochrome c release, while residing in mitochondria and associating with adenine nucleotide translocator. ammonium ferrous sulfate 49-52 caspase 8 Homo sapiens 97-106 10508784-1 1999 BACKGROUND: In the initial stages of Fas-mediated apoptosis the cysteine protease caspase-8 is recruited to the cell receptor as a zymogen (procaspase-8) and is incorporated into the death-signalling complex. ammonium ferrous sulfate 37-40 caspase 8 Homo sapiens 82-91 10508785-3 1999 Caspase-8 is designated as an initiator caspase and is believed to sit at the apex of the Fas- or TNF-mediated apoptotic cascade. ammonium ferrous sulfate 90-93 caspase 8 Homo sapiens 0-9 10477698-8 1999 Our results imply that the relative levels of caspase-8 and FLIP(L) are an important determinant of susceptibility to Fas-mediated apoptosis. ammonium ferrous sulfate 118-121 caspase 8 Homo sapiens 46-55 10444259-2 1999 In this study, we have examined the role of downstream mediators, including caspases, in Fas-mediated apoptosis in lymphocytes from ageing humans. ammonium ferrous sulfate 89-92 caspase 8 Homo sapiens 76-84 10328578-3 1999 We have recently found that Fas-mediated apoptosis of RA synoviocytes is associated with activation of two signaling pathways, the c-Jun amino-terminal kinase (JNK)/activator protein-1 (AP-1) pathway, and the FADD (Fas-associated death domain protein)/Caspase-8/Caspase-3/PARP (poly(ADP-ribose)polymerase) pathway. ammonium ferrous sulfate 28-31 caspase 8 Homo sapiens 252-261 9971775-7 1999 In addition, we show that HIV-1Deltaenv infection and Tat expression increased the sensitivity of cells to Fas-mediated apoptosis, an apoptotic pathway that signals via casp-8. ammonium ferrous sulfate 107-110 caspase 8 Homo sapiens 169-175 10066378-5 1999 This suggests that an upstream caspase such as caspase-8 is required for the Fas-mediated release of mitochondrial cytochrome c. ammonium ferrous sulfate 77-80 caspase 8 Homo sapiens 31-38 10066378-5 1999 This suggests that an upstream caspase such as caspase-8 is required for the Fas-mediated release of mitochondrial cytochrome c. ammonium ferrous sulfate 77-80 caspase 8 Homo sapiens 47-56 10088775-10 1999 A gradual up-regulation of caspase 8 and caspase 3, which played a role in the caspase cascade for Fas-mediated apoptosis, was observed in TNFalpha-treated cultured OA synoviocytes. ammonium ferrous sulfate 99-102 caspase 8 Homo sapiens 27-36 10088775-12 1999 More importantly, Z-IETD-FMK, a caspase 8 inhibitor, and Ac-DEVD-CHO, a caspase 3 inhibitor, almost completely inhibited Fas-mediated apoptosis of TNFalpha-treated cultured OA synoviocytes, whereas Ac-YVAD-CHO, a caspase 1 inhibitor, did not. ammonium ferrous sulfate 121-124 caspase 8 Homo sapiens 32-41 9973403-7 1999 Overexpression of TAL accelerated Fas-induced mitochondrial ROI production, Deltapsim elevation, activation of caspase-8 and caspase-3, proteolysis of poly(A)DP-ribose polymerase, and PS externalization. ammonium ferrous sulfate 34-37 caspase 8 Homo sapiens 111-120 9880544-5 1999 BTK associates with Fas via its kinase and PH domains and prevents the FAS-FADD interaction, which is essential for the recruitment and activation of FLICE by Fas during the apoptotic signal. ammonium ferrous sulfate 20-23 caspase 8 Homo sapiens 150-155 9891071-6 1999 Consistent with these observations, Fas-induced apoptosis in Jurkat cells resulted in caspase 8 activation and subsequently in activation of Cif. ammonium ferrous sulfate 36-39 caspase 8 Homo sapiens 86-95 9880544-5 1999 BTK associates with Fas via its kinase and PH domains and prevents the FAS-FADD interaction, which is essential for the recruitment and activation of FLICE by Fas during the apoptotic signal. ammonium ferrous sulfate 159-162 caspase 8 Homo sapiens 150-155 9606216-0 1998 E1B 19K inhibits Fas-mediated apoptosis through FADD-dependent sequestration of FLICE. ammonium ferrous sulfate 17-20 caspase 8 Homo sapiens 80-85 9840917-3 1998 In this report, we show that Fas activation induced the processing of caspase 8 in Jurkat cells with a time frame similar to the activation of caspase 3 and the proteolysis of nuclear proteins. ammonium ferrous sulfate 29-32 caspase 8 Homo sapiens 70-79 9840917-6 1998 Furthermore, although caspase 8 was recruited to Fas upon Fas activation in the parental Jurkat cells, the recruitment of caspase 8 to Fas was inhibited in the transformants overexpressing Bcl-2. ammonium ferrous sulfate 49-52 caspase 8 Homo sapiens 22-31 9840917-6 1998 Furthermore, although caspase 8 was recruited to Fas upon Fas activation in the parental Jurkat cells, the recruitment of caspase 8 to Fas was inhibited in the transformants overexpressing Bcl-2. ammonium ferrous sulfate 58-61 caspase 8 Homo sapiens 22-31 9840917-6 1998 Furthermore, although caspase 8 was recruited to Fas upon Fas activation in the parental Jurkat cells, the recruitment of caspase 8 to Fas was inhibited in the transformants overexpressing Bcl-2. ammonium ferrous sulfate 58-61 caspase 8 Homo sapiens 22-31 9840917-7 1998 These results suggest that Bcl-2 inhibits Fas-induced apoptosis by preventing the formation of the death-inducing signaling complex that is composed of Fas, FADD/MORT1, and caspase 8. ammonium ferrous sulfate 42-45 caspase 8 Homo sapiens 173-182 9865748-0 1998 Fas-mediated apoptosis in human prostatic carcinoma cell lines occurs via activation of caspase-8 and caspase-7. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 88-97 9865748-6 1998 In experiments with caspase inhibitors we show that Fas-mediated apoptosis in PC3 is mainly executed by the caspase-3 subfamily, but another member(s) of the caspase family may be involved in Fas-mediated apoptosis in ALVA31, DU145, and JCA1. ammonium ferrous sulfate 52-55 caspase 8 Homo sapiens 20-27 9865748-6 1998 In experiments with caspase inhibitors we show that Fas-mediated apoptosis in PC3 is mainly executed by the caspase-3 subfamily, but another member(s) of the caspase family may be involved in Fas-mediated apoptosis in ALVA31, DU145, and JCA1. ammonium ferrous sulfate 52-55 caspase 8 Homo sapiens 108-115 9865748-6 1998 In experiments with caspase inhibitors we show that Fas-mediated apoptosis in PC3 is mainly executed by the caspase-3 subfamily, but another member(s) of the caspase family may be involved in Fas-mediated apoptosis in ALVA31, DU145, and JCA1. ammonium ferrous sulfate 192-195 caspase 8 Homo sapiens 20-27 9865748-8 1998 The activated form of caspase-8 was detected in DU145 only after 4 h of simultaneous treatment with CHX and anti-Fas mAb, whereas in PC3 caspase-8 was found to be activated after 1 h of Fas-ligation. ammonium ferrous sulfate 113-116 caspase 8 Homo sapiens 22-31 9740801-0 1998 Essential requirement for caspase-8/FLICE in the initiation of the Fas-induced apoptotic cascade. ammonium ferrous sulfate 67-70 caspase 8 Homo sapiens 26-35 9740801-0 1998 Essential requirement for caspase-8/FLICE in the initiation of the Fas-induced apoptotic cascade. ammonium ferrous sulfate 67-70 caspase 8 Homo sapiens 36-41 9740801-5 1998 RESULTS: In a genetic screen for essential components of the Fas-mediated apoptotic cascade, we isolated a Jurkat T lymphocyte cell line deficient in caspase-8 that was completely resistant to Fas-induced apoptosis. ammonium ferrous sulfate 61-64 caspase 8 Homo sapiens 150-159 9740801-5 1998 RESULTS: In a genetic screen for essential components of the Fas-mediated apoptotic cascade, we isolated a Jurkat T lymphocyte cell line deficient in caspase-8 that was completely resistant to Fas-induced apoptosis. ammonium ferrous sulfate 193-196 caspase 8 Homo sapiens 150-159 9740801-7 1998 Fas activation of multiple caspases and of the stress kinase p38 and c-Jun NH2-terminal kinase (JNK) was completely blocked in the caspase-8-deficient cell line. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 27-35 9740801-7 1998 Fas activation of multiple caspases and of the stress kinase p38 and c-Jun NH2-terminal kinase (JNK) was completely blocked in the caspase-8-deficient cell line. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 131-140 9740801-9 1998 CONCLUSIONS: This study provides the first genetic evidence that caspase-8 occupies an essential and apical position in the Fas signaling pathway and suggests that caspase-8 may participate broadly in multiple apoptotic pathways. ammonium ferrous sulfate 124-127 caspase 8 Homo sapiens 65-74 9740801-9 1998 CONCLUSIONS: This study provides the first genetic evidence that caspase-8 occupies an essential and apical position in the Fas signaling pathway and suggests that caspase-8 may participate broadly in multiple apoptotic pathways. ammonium ferrous sulfate 124-127 caspase 8 Homo sapiens 164-173 9694885-10 1998 Our results demonstrate that whereas Fas-mediated activation of caspase 3 requires an upstream caspase activity that is zVAD-fmk-sensitive, the initial cleavage of caspase 3 during granule-mediated cell death is insensitive to zVAD-fmk, suggesting that caspase 3 is cleaved directly by granzyme B in vivo. ammonium ferrous sulfate 37-40 caspase 8 Homo sapiens 64-71 9606216-8 1998 Thus, E1B 19K may inhibit Fas-mediated cell death downstream of FADD recruitment of FLICE but upstream of FLICE activation by disrupting FADD oligomerization and sequestering an essential component of the DISC. ammonium ferrous sulfate 26-29 caspase 8 Homo sapiens 84-89 27746017-4 2016 Extensive assembly interfaces not predicted by the previously proposed linear DED chain model were uncovered, and were further confirmed by structure-based mutagenesis in filament formation in vitro and Fas-induced apoptosis and ASC-mediated caspase-8 recruitment in cells. ammonium ferrous sulfate 203-206 caspase 8 Homo sapiens 242-251 9654089-2 1998 In apoptosis triggered by Fas, a subset of cysteine proteases designated caspases is activated, playing a central role as effector molecules. ammonium ferrous sulfate 26-29 caspase 8 Homo sapiens 73-81 9654089-3 1998 Among these caspases, human caspase-8 (FLICE/MACH/Mch5) has been isolated and shown to be indispensable for Fas-mediated apoptotic signaling. ammonium ferrous sulfate 108-111 caspase 8 Homo sapiens 12-20 9654089-3 1998 Among these caspases, human caspase-8 (FLICE/MACH/Mch5) has been isolated and shown to be indispensable for Fas-mediated apoptotic signaling. ammonium ferrous sulfate 108-111 caspase 8 Homo sapiens 28-37 9654089-3 1998 Among these caspases, human caspase-8 (FLICE/MACH/Mch5) has been isolated and shown to be indispensable for Fas-mediated apoptotic signaling. ammonium ferrous sulfate 108-111 caspase 8 Homo sapiens 39-44 9654089-3 1998 Among these caspases, human caspase-8 (FLICE/MACH/Mch5) has been isolated and shown to be indispensable for Fas-mediated apoptotic signaling. ammonium ferrous sulfate 108-111 caspase 8 Homo sapiens 50-54 9446604-1 1998 The assembly of the CD-95 (Fas/Apo-1) receptor death-inducing signaling complex occurs in a hierarchical manner; the death domain of CD-95 binds to the corresponding domain in the adapter molecule Fas-associated death domain (FADD) Mort-1, which in turn recruits the zymogen form of the death protease caspase-8 (FLICE/Mach-1) by a homophilic interaction involving the death effector domains. ammonium ferrous sulfate 27-30 caspase 8 Homo sapiens 302-311 9446604-1 1998 The assembly of the CD-95 (Fas/Apo-1) receptor death-inducing signaling complex occurs in a hierarchical manner; the death domain of CD-95 binds to the corresponding domain in the adapter molecule Fas-associated death domain (FADD) Mort-1, which in turn recruits the zymogen form of the death protease caspase-8 (FLICE/Mach-1) by a homophilic interaction involving the death effector domains. ammonium ferrous sulfate 27-30 caspase 8 Homo sapiens 313-318 28000850-6 2017 The present study demonstrated that Fas was associated with gastric cancer and promoted the apoptosis of gastric cancer cells via caspase-8, caspase-3 and PARP1. ammonium ferrous sulfate 36-39 caspase 8 Homo sapiens 130-139 9190889-3 1997 YV(bio)KD-aomk identified six distinct polypeptides corresponding to active caspases in Fas-stimulated Jurkat T cells. ammonium ferrous sulfate 88-91 caspase 8 Homo sapiens 76-84 9190889-9 1997 Overall, these findings provide evidence that caspase-8, a CrmA-sensitive protease, is responsible for initiating the stepwise activation of multiple caspases in Fas-stimulated cells. ammonium ferrous sulfate 162-165 caspase 8 Homo sapiens 46-55 9190889-9 1997 Overall, these findings provide evidence that caspase-8, a CrmA-sensitive protease, is responsible for initiating the stepwise activation of multiple caspases in Fas-stimulated cells. ammonium ferrous sulfate 162-165 caspase 8 Homo sapiens 150-158 30900802-12 2019 Induction of extrinsic Fas-mediated apoptosis is verified by Western blotting for cleaved caspase 8, the initiator caspase of extrinsic apoptosis. ammonium ferrous sulfate 23-26 caspase 8 Homo sapiens 90-99 31168030-0 2019 The anti-cancer drug gefitinib accelerates Fas-mediated apoptosis by enhancing caspase-8 activation in cancer cells. ammonium ferrous sulfate 43-46 caspase 8 Homo sapiens 79-88 31168030-4 2019 In the presence of nontoxic concentrations of gefitinib, Fas-induced activation of caspase-8 and subsequent apoptosis was dramatically promoted, suggesting that gefitinib increases the sensitivity to Fas-mediated apoptosis. ammonium ferrous sulfate 57-60 caspase 8 Homo sapiens 83-92 31168030-4 2019 In the presence of nontoxic concentrations of gefitinib, Fas-induced activation of caspase-8 and subsequent apoptosis was dramatically promoted, suggesting that gefitinib increases the sensitivity to Fas-mediated apoptosis. ammonium ferrous sulfate 200-203 caspase 8 Homo sapiens 83-92 30061622-10 2018 Therefore, we demonstrated that a novel Fas-binding OMP and LPS of L. interrogans induce macrophage apoptosis through the Fas/FasL-caspase-8/-3 pathway. ammonium ferrous sulfate 40-43 caspase 8 Homo sapiens 131-140 28302628-3 2017 The experiments showed significantly increased releases of FADD, caspase-3, and caspase-8 after Fas stimulation. ammonium ferrous sulfate 96-99 caspase 8 Homo sapiens 80-89 27748905-9 2016 Taken together, our data indicated that 8-ADEQ-stimulated apoptosis in HL-60 leukemia cells is due to a Fas-mediated caspase-8-dependent pathway via ROS generation, but also, to a lesser extent cytochrome c release and caspase-9 activation. ammonium ferrous sulfate 104-107 caspase 8 Homo sapiens 117-126 24442508-7 2014 RESULTS: hIAPP treatment induced activation of caspase-8 and -3 in islet beta cells (via Fas upregulation), resulting in apoptosis, which was markedly reduced by blocking caspase-8, Fas or amyloid. ammonium ferrous sulfate 89-92 caspase 8 Homo sapiens 47-63 27622714-7 2016 In contrast, in functional p53-impaired cells, this phytochemical exploits p53-paralogue p73, which up-regulates FAS to cleave BID through FAS-FADD-caspase-8-pathway. ammonium ferrous sulfate 113-116 caspase 8 Homo sapiens 148-157 26796280-3 2016 The triple-negative breast cancer, MDA-MB-231, overexpresses the protein Lifeguard, which inhibits Fas-mediated apoptosis by inhibiting Caspase-8 activation after Fas trimerization. ammonium ferrous sulfate 99-102 caspase 8 Homo sapiens 136-145 26796280-3 2016 The triple-negative breast cancer, MDA-MB-231, overexpresses the protein Lifeguard, which inhibits Fas-mediated apoptosis by inhibiting Caspase-8 activation after Fas trimerization. ammonium ferrous sulfate 163-166 caspase 8 Homo sapiens 136-145 25434584-10 2015 Icaritin also activated the Fas-mediated apoptosis pathway, as was evident by the increased expression levels of Fas and activation of caspase-8. ammonium ferrous sulfate 28-31 caspase 8 Homo sapiens 135-144 25695505-11 2015 Collectively, these data suggest that caspase-4 activity is required for Fas-induced cell apoptosis and caspase-4 may act upstream of PARP and caspase-3 and downstream of caspase-8. ammonium ferrous sulfate 73-76 caspase 8 Homo sapiens 171-180 24535941-3 2014 In this study, we investigated the association of four polymorphisms (FAS -1377G>A, -670A>G, FASL -844C>T, and CASP8 -652 6N ins>del) with the clinical outcome of 940 gastric cancer patients in a Chinese population. ammonium ferrous sulfate 70-73 caspase 8 Homo sapiens 120-125 24484936-6 2014 Using an RNAi-based strategy we could demonstrate that the extrinsic cell death is increased upon Fas-stimulation when endogenous caspase-8 is replaced by a mutant (S305A) mimicking the non-phosphorylated form. ammonium ferrous sulfate 98-101 caspase 8 Homo sapiens 130-139 24442508-7 2014 RESULTS: hIAPP treatment induced activation of caspase-8 and -3 in islet beta cells (via Fas upregulation), resulting in apoptosis, which was markedly reduced by blocking caspase-8, Fas or amyloid. ammonium ferrous sulfate 89-92 caspase 8 Homo sapiens 47-56 22806078-0 2013 FasL-triggered death of Jurkat cells requires caspase 8-induced, ATP-dependent cross-talk between Fas and the purinergic receptor P2X(7). ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 46-55 23590971-7 2013 Although the ligation of Fas results in caspase-8 cleavage and ERK1/2 phosphorylation, inhibitors for caspase-8 and MEK have no effect on the expressions of RORgammat, IL-17A, and IL-17F. ammonium ferrous sulfate 25-28 caspase 8 Homo sapiens 40-49 22992806-6 2013 The resulting increase in nitric oxide significantly upregulates keratinocyte FasL expression, resulting in Fas- and caspase-8-mediated keratinocyte cell death. ammonium ferrous sulfate 78-81 caspase 8 Homo sapiens 117-126 22806078-1 2013 Fas ligation via the ligand FasL activates the caspase-8/caspase-3-dependent extrinsic death pathway. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 47-56 22266324-6 2012 Moreover, diosgenin inhibited FLIP and activated caspase-8 in the FAS-related apoptotic pathway. ammonium ferrous sulfate 66-69 caspase 8 Homo sapiens 49-58 23144495-0 2012 Cutting edge: FAS (CD95) mediates noncanonical IL-1beta and IL-18 maturation via caspase-8 in an RIP3-independent manner. ammonium ferrous sulfate 14-17 caspase 8 Homo sapiens 81-90 22492309-6 2012 The therapeutical effect of sulfuretin in leukemia is due to its potent apoptotic activity through the extrinsic pathway driven by a Fas-mediated caspase-8-dependent pathway. ammonium ferrous sulfate 133-136 caspase 8 Homo sapiens 146-155 22611243-0 2012 Signal-transducing adaptor protein-2 modulates Fas-mediated T cell apoptosis by interacting with caspase-8. ammonium ferrous sulfate 47-50 caspase 8 Homo sapiens 97-106 22611243-4 2012 Moreover, STAP-2 protein has a consensus caspase-8 cleavage sequence, VEAD, in its C-terminal domain, and processing of STAP-2 by caspase-8 was crucial for Fas-induced apoptosis. ammonium ferrous sulfate 156-159 caspase 8 Homo sapiens 41-50 22611243-4 2012 Moreover, STAP-2 protein has a consensus caspase-8 cleavage sequence, VEAD, in its C-terminal domain, and processing of STAP-2 by caspase-8 was crucial for Fas-induced apoptosis. ammonium ferrous sulfate 156-159 caspase 8 Homo sapiens 130-139 24024120-3 2012 Here we show that switching off the Fas pathway using Fas apoptotic inhibitory protein (Faim/TOSO), which regulates apoptosis upstream of caspase 8, blocked beta-cell apoptosis and increased proliferation in human islets. ammonium ferrous sulfate 36-39 caspase 8 Homo sapiens 138-147 22019693-6 2012 Additionally, cinobufacini also activated Fas-mediated apoptosis pathway obviously as evident by an increase in Fas expression, and caspase-8 and caspase-10 activation. ammonium ferrous sulfate 42-45 caspase 8 Homo sapiens 132-141 21959933-2 2012 Fas-induced apoptosis requires recruitment and activation of the initiator caspase, caspase-8 (in humans also caspase-10), within the death-inducing signalling complex. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 75-82 21959933-2 2012 Fas-induced apoptosis requires recruitment and activation of the initiator caspase, caspase-8 (in humans also caspase-10), within the death-inducing signalling complex. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 84-93 21191032-6 2011 Using activated caspase 3 and caspase 8 as indicators of apoptosis, flow cytometry, confocal microscopy, and Western blot analyses demonstrated that inflammation-induced MDSC treated with a Fas agonist contain lower levels of activated caspases, suggesting that inflammation enhances resistance to Fas-mediated apoptosis. ammonium ferrous sulfate 190-193 caspase 8 Homo sapiens 30-39 23285096-6 2012 However, GTP-induced FAS upregulation through activation of c-jun-N-terminal kinase resulted in FADD phosphorylation, caspase-8 activation and truncation of BID, leading to apoptosis in both LNCaPshV and LNCaPshp53 cells. ammonium ferrous sulfate 21-24 caspase 8 Homo sapiens 118-127 22357491-8 2011 CONCLUSION: CASP8-652 6N DD genotype may play a role in CWP development and interact with SNPs of FAS-1377, FAS-670 and FASL-844. ammonium ferrous sulfate 98-101 caspase 8 Homo sapiens 12-17 22357491-8 2011 CONCLUSION: CASP8-652 6N DD genotype may play a role in CWP development and interact with SNPs of FAS-1377, FAS-670 and FASL-844. ammonium ferrous sulfate 108-111 caspase 8 Homo sapiens 12-17 21478427-3 2011 Whereas FAS-induced apoptosis was followed by caspase-8 activation and required Bid to initiate the mitochondrial amplification loop, TNF-alpha-induced apoptosis involved class IA PI3Ks, which were activated by MAPK p38. ammonium ferrous sulfate 8-11 caspase 8 Homo sapiens 46-55 21183680-4 2011 Finally, RSK2 blocks Fas-induced apoptosis through its phosphorylation of caspase-8. ammonium ferrous sulfate 21-24 caspase 8 Homo sapiens 74-83 21926110-7 2011 Haplotype analysis indicated that the FAS polymorphisms -670 A > G (rs1800682) and -1377 G > A (rs2234767) were both associated with OSCC in the Mixed Ancestry population (P = 0.006 and P = 0.004, respectively), as well as the CASP8 (-652 6Ndel:302His) haplotype (P = 0.0013). ammonium ferrous sulfate 38-41 caspase 8 Homo sapiens 233-238 21191032-6 2011 Using activated caspase 3 and caspase 8 as indicators of apoptosis, flow cytometry, confocal microscopy, and Western blot analyses demonstrated that inflammation-induced MDSC treated with a Fas agonist contain lower levels of activated caspases, suggesting that inflammation enhances resistance to Fas-mediated apoptosis. ammonium ferrous sulfate 190-193 caspase 8 Homo sapiens 236-244 20421362-3 2010 From the cells surviving apoptogenic Fas stimulation, four candidate shRNA sequences were obtained that provided resistance to Fas-induced cell death, including two shRNAs for caspase-8, an shRNA for Bid, and an shRNA for Fas. ammonium ferrous sulfate 127-130 caspase 8 Homo sapiens 176-185 20937773-0 2010 Cdk1/cyclin B1 controls Fas-mediated apoptosis by regulating caspase-8 activity. ammonium ferrous sulfate 24-27 caspase 8 Homo sapiens 61-70 20510339-3 2010 Fas-induced apoptosis can be restored in these cells by up-regulation of caspase 8 expression by means of transient transfection with a caspase 8-encoding plasmid. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 73-82 20510339-3 2010 Fas-induced apoptosis can be restored in these cells by up-regulation of caspase 8 expression by means of transient transfection with a caspase 8-encoding plasmid. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 136-145 19626005-3 2009 In type I cells, such as lymphocytes, activation of "effector caspases" by FAS-induced activation of caspase-8 suffices for cell killing, whereas in type II cells, including hepatocytes and pancreatic beta-cells, caspase cascade amplification through caspase-8-mediated activation of the pro-apoptotic BCL-2 family member BID (BH3 interacting domain death agonist) is essential. ammonium ferrous sulfate 75-78 caspase 8 Homo sapiens 62-70 20046832-5 2009 In the lipid rafts, Fas interacts with Fas-associated death domain (FADD) and Caspase-8 to form death-inducing signaling complex (DISC) and thus mediates cell apoptosis. ammonium ferrous sulfate 20-23 caspase 8 Homo sapiens 78-87 19710364-5 2009 Mib1 was found to bind to cellular Fas-associated death domain (FADD)-like IL-1b converting enzyme (FLICE)-like inhibitory proteins (cFLIP-L and cFLIP-S), whereas only cFLIP-s can inhibit Mib1-induced cell death. ammonium ferrous sulfate 35-38 caspase 8 Homo sapiens 100-105 19906200-4 2009 Fas-induced apoptosis was dependent on the caspase-8 activation, since the overexpression of c-FLIP(long), a natural inhibitor of caspase-8 activation, blocked apoptosis induced by Fas. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 43-52 19906200-4 2009 Fas-induced apoptosis was dependent on the caspase-8 activation, since the overexpression of c-FLIP(long), a natural inhibitor of caspase-8 activation, blocked apoptosis induced by Fas. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 130-139 19906200-4 2009 Fas-induced apoptosis was dependent on the caspase-8 activation, since the overexpression of c-FLIP(long), a natural inhibitor of caspase-8 activation, blocked apoptosis induced by Fas. ammonium ferrous sulfate 181-184 caspase 8 Homo sapiens 43-52 19906200-4 2009 Fas-induced apoptosis was dependent on the caspase-8 activation, since the overexpression of c-FLIP(long), a natural inhibitor of caspase-8 activation, blocked apoptosis induced by Fas. ammonium ferrous sulfate 181-184 caspase 8 Homo sapiens 130-139 19628709-7 2009 NF-kappaB up-regulates Fas and predisposes to Fas-induced cell death, which is caspase-8 mediated and can be prevented by CFLAR overexpression. ammonium ferrous sulfate 23-26 caspase 8 Homo sapiens 79-88 19628709-7 2009 NF-kappaB up-regulates Fas and predisposes to Fas-induced cell death, which is caspase-8 mediated and can be prevented by CFLAR overexpression. ammonium ferrous sulfate 46-49 caspase 8 Homo sapiens 79-88 19626005-3 2009 In type I cells, such as lymphocytes, activation of "effector caspases" by FAS-induced activation of caspase-8 suffices for cell killing, whereas in type II cells, including hepatocytes and pancreatic beta-cells, caspase cascade amplification through caspase-8-mediated activation of the pro-apoptotic BCL-2 family member BID (BH3 interacting domain death agonist) is essential. ammonium ferrous sulfate 75-78 caspase 8 Homo sapiens 101-110 19626005-3 2009 In type I cells, such as lymphocytes, activation of "effector caspases" by FAS-induced activation of caspase-8 suffices for cell killing, whereas in type II cells, including hepatocytes and pancreatic beta-cells, caspase cascade amplification through caspase-8-mediated activation of the pro-apoptotic BCL-2 family member BID (BH3 interacting domain death agonist) is essential. ammonium ferrous sulfate 75-78 caspase 8 Homo sapiens 62-69 19626005-3 2009 In type I cells, such as lymphocytes, activation of "effector caspases" by FAS-induced activation of caspase-8 suffices for cell killing, whereas in type II cells, including hepatocytes and pancreatic beta-cells, caspase cascade amplification through caspase-8-mediated activation of the pro-apoptotic BCL-2 family member BID (BH3 interacting domain death agonist) is essential. ammonium ferrous sulfate 75-78 caspase 8 Homo sapiens 251-260 19626005-4 2009 Here we show that loss of XIAP (X-chromosome linked inhibitor of apoptosis protein) function by gene targeting or treatment with a second mitochondria-derived activator of caspases (SMAC, also called DIABLO; direct IAP-binding protein with low pI) mimetic drug in mice rendered hepatocytes and beta-cells independent of BID for FAS-induced apoptosis. ammonium ferrous sulfate 328-331 caspase 8 Homo sapiens 172-180 19240112-4 2009 When cells are exposed to Fas-related signals, the ubiquitously expressed p21-activated kinase 2 (Pak2) can be activated via cleavage by caspases to release a constitutively active C-terminal fragment, which mediates cell death. ammonium ferrous sulfate 26-29 caspase 8 Homo sapiens 137-145 19496190-7 2009 Additionally, ethanol-treated cells displayed enhanced susceptibility to Fas-mediated apoptosis that was blocked by GSH depletion as a result of diminished caspase-8 activity. ammonium ferrous sulfate 73-76 caspase 8 Homo sapiens 156-165 19360334-5 2009 Our studies show that knockdown of STAT3 expression by siRNA reduced expression of Bcl-xL and survivin in MDA-MB-231 cells, and also led to Fas mediated intrinsic apoptotic pathway by activating caspases -8, -9, -3 and PARP1 cleavage. ammonium ferrous sulfate 140-143 caspase 8 Homo sapiens 195-214 19239902-5 2009 FAS triggers apoptosis through FADD-mediated recruitment and activation of caspase-8. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 75-84 19006686-5 2009 This study reports novel evidence, which demonstrates that chronic extrinsic cervical spinal cord compression leads to Fas-mediated apoptosis of neurons and oligodendrocytes which is associated with activation of caspase-8, -9 and -3 and progressive neurological deficits. ammonium ferrous sulfate 119-122 caspase 8 Homo sapiens 213-233 18922907-5 2008 The proapoptotic effects of this treatment combination were abrogated by a caspase-8 inhibitor, led to increased association of Fas and FADD before the onset of cell death, and were significantly reduced in cells transfected with a dominant-negative FADD construct or small interfering RNA targeting Fas. ammonium ferrous sulfate 128-131 caspase 8 Homo sapiens 75-84 19135479-6 2009 FasL linking to Fas on DCs triggers the activation of caspase-8, which eventually leads to mitochondria-mediated apoptosis via truncation of BID. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 54-63 19940360-0 2009 Fas-induced apoptosis of renal cell carcinoma is mediated by apoptosis signal-regulating kinase 1 via mitochondrial damage-dependent caspase-8 activation. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 133-142 19099630-11 2008 The possible mechanism is supposed to be up-regulating Fas, down-regulating FasL levels and then activating the levels of caspase 8 and caspase 3. ammonium ferrous sulfate 55-58 caspase 8 Homo sapiens 122-131 18615109-2 2008 We have shown that death of these precursors involved the Fas-dependent activation of caspase-8. ammonium ferrous sulfate 58-61 caspase 8 Homo sapiens 86-95 18448526-9 2008 Fas immunoprecipitation detected FADD (Fas-associated death domain protein) and caspase 8, suggesting a Fas-dependent formation of the death-inducing signaling complex. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 80-89 18622903-9 2008 These findings suggest that tanshinone IIA induces apoptosis in Colo-205 cells through both mitochondrial-mediated intrinsic and Fas-mediated extrinsic caspase cell-death pathways. ammonium ferrous sulfate 129-132 caspase 8 Homo sapiens 152-159 17689285-4 2008 In OSCC cells treated with EGFR inhibitors, Fas-mediated apoptosis was accompanied by caspase-8 activation but not Bid cleavage. ammonium ferrous sulfate 44-47 caspase 8 Homo sapiens 86-95 18375387-4 2008 Lansoprazole also inhibits indomethacin-induced Fas-mediated mucosal cell death by down-regulating Fas or FasL expression and inhibiting caspase-8 activation. ammonium ferrous sulfate 48-51 caspase 8 Homo sapiens 137-146 17453339-10 2008 We also found that inhibition of cFLIP(L) expression in T47D cells decreased Fas-mediated caspase-8 activation and activation of effector caspases. ammonium ferrous sulfate 77-80 caspase 8 Homo sapiens 90-99 17453339-11 2008 We propose that in T47D p43 cFLIP(L) in the Fas-DISC may promote caspase-8 activation. ammonium ferrous sulfate 44-47 caspase 8 Homo sapiens 65-74 17973862-9 2007 In CH-11-resistant cells, cellular Fas-associated death domain-like interleukin-1b-converting enzyme inhibitory protein (c-FLIP) proteins were recruited to the DISC, resulting in inhibition of caspase 8 and caspase 10 cleavage. ammonium ferrous sulfate 35-38 caspase 8 Homo sapiens 193-202 18250974-3 2008 Fas engagement drives the formation of a complex termed DISC (death-inducing signaling complex), which contains the adaptor molecule Fas-associated protein, two members of the caspase family caspase-8 and -10, and a pseudo-caspase termed c-FLIP. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 176-183 18250974-3 2008 Fas engagement drives the formation of a complex termed DISC (death-inducing signaling complex), which contains the adaptor molecule Fas-associated protein, two members of the caspase family caspase-8 and -10, and a pseudo-caspase termed c-FLIP. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 191-208 17893514-8 2007 In view of these results and taking into consideration that K562 cells are Fas-null, we suggested that caspase-8 is activated in a Fas-independent manner downstream of caspase-9. ammonium ferrous sulfate 75-78 caspase 8 Homo sapiens 103-112 17893514-8 2007 In view of these results and taking into consideration that K562 cells are Fas-null, we suggested that caspase-8 is activated in a Fas-independent manner downstream of caspase-9. ammonium ferrous sulfate 131-134 caspase 8 Homo sapiens 103-112 17970047-8 2007 The significance of the above changes was confirmed by the detection of considerably more caspase-8 in both the Fas or FADD immunoprecipitate obtained from cells treated with the doxorubicin/selenium combination. ammonium ferrous sulfate 112-115 caspase 8 Homo sapiens 90-99 17222828-2 2007 Conversely, GSH depletion by the aldehyde acrolein (3-30 microM) was associated with inhibition of Fas-induced caspase-8 activation, although GSH depletion by buthionine sulfoximine (BSO) did not affect caspase-8 activation. ammonium ferrous sulfate 99-102 caspase 8 Homo sapiens 111-120 17898872-3 2007 Caspase-8 was found to interact with Fas and DR4 receptors on narciclasine treatment. ammonium ferrous sulfate 37-40 caspase 8 Homo sapiens 0-9 17277134-11 2007 In conclusion, in this study, we present evidence for the involvement of the death receptor pathway in rituximab-induced apoptosis of Ramos B cells with concomitant sensitization of these cells to Fas-mediated apoptosis via Fas multimerization and recruitment of caspase-8 and FADD to the DISC. ammonium ferrous sulfate 197-200 caspase 8 Homo sapiens 263-272 17561974-0 2007 Cell cycle dependency of caspase activation in Fas-induced apoptosis in leukemia cells. ammonium ferrous sulfate 47-50 caspase 8 Homo sapiens 25-32 17561974-2 2007 In the present study we have investigated the relationship between cell cycle progression and the activation of caspases (caspase-3 and caspase-8) in Fas (CD95)-mediated apoptosis in asynchronously growing leukemia cells. ammonium ferrous sulfate 150-153 caspase 8 Homo sapiens 112-120 17561974-2 2007 In the present study we have investigated the relationship between cell cycle progression and the activation of caspases (caspase-3 and caspase-8) in Fas (CD95)-mediated apoptosis in asynchronously growing leukemia cells. ammonium ferrous sulfate 150-153 caspase 8 Homo sapiens 136-145 17549401-6 2007 Activation of caspase 8 accompanied by the release of cytochrome c and cleavage of PARP was also observed and indicative of Fas-mediated apoptosis. ammonium ferrous sulfate 124-127 caspase 8 Homo sapiens 14-23 17393415-6 2007 Apoptosis induced by Fas in FLS was detected with TUNEL staining and Western blotting of caspase 8 and poly(ADP-ribose) polymerase. ammonium ferrous sulfate 21-24 caspase 8 Homo sapiens 89-98 17277134-2 2007 In this study, we demonstrate that rituximab sensitizes lymphoma B cells to Fas-induced apoptosis in a caspase-8-dependent manner. ammonium ferrous sulfate 76-79 caspase 8 Homo sapiens 103-112 16646028-0 2006 Fas activation of a proinflammatory program in rheumatoid synoviocytes and its regulation by FLIP and caspase 8 signaling. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 102-111 16806159-10 2006 Although it has been suggested that Fas-mediated signaling may contribute to the caspase-8 activation induced by DNA-damaging agents; however, bromovulone III did not induce any DNA breakage, suggesting that bromovulone III-induced Fas/caspase-8-dependent signaling is not through the direct target on DNA damage. ammonium ferrous sulfate 36-39 caspase 8 Homo sapiens 81-90 16619028-3 2006 Src activation triggers Caspase-8 phosphorylation on Tyr380 and impairs Fas-induced apoptosis. ammonium ferrous sulfate 72-75 caspase 8 Homo sapiens 24-33 16493077-10 2006 These data suggest that C5b-9 through PI3K signaling can rescue OLG from Fas-mediated apoptosis by regulating caspase-8 processing. ammonium ferrous sulfate 73-76 caspase 8 Homo sapiens 110-119 16330535-0 2006 Fas costimulation of naive CD4 T cells is controlled by NF-kappaB signaling and caspase activity. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 80-87 16330535-7 2006 Thus, Fas-mediated costimulation of naive CD4 T cells is driven by active caspases, and NF-kappaB acts as a dominant survival-supporting factor of Fas-costimulated cells containing high levels of activated caspase-8 and -3. ammonium ferrous sulfate 6-9 caspase 8 Homo sapiens 74-82 16330535-7 2006 Thus, Fas-mediated costimulation of naive CD4 T cells is driven by active caspases, and NF-kappaB acts as a dominant survival-supporting factor of Fas-costimulated cells containing high levels of activated caspase-8 and -3. ammonium ferrous sulfate 6-9 caspase 8 Homo sapiens 206-222 16364918-1 2005 The death-inducing signaling complex (DISC) comprising Fas, Fas-associated death domain (FADD), and caspase-8/10 is assembled via homotypic associations between death domains (DDs) of Fas and FADD and between death effector domains (DEDs) of FADD and caspase-8/10. ammonium ferrous sulfate 60-63 caspase 8 Homo sapiens 251-260 16246840-1 2005 Stimulation of cell surface Fas (CD95) results in recruitment of cytoplasmic proteins and activation of caspase-8, which in turn activates downstream effector caspases leading to programmed cell death. ammonium ferrous sulfate 28-31 caspase 8 Homo sapiens 104-113 16246840-0 2005 Nitric oxide negatively regulates Fas CD95-induced apoptosis through inhibition of ubiquitin-proteasome-mediated degradation of FLICE inhibitory protein. ammonium ferrous sulfate 34-37 caspase 8 Homo sapiens 128-133 15924153-7 2005 Interestingly, cotreatment of Hank-1 with cycloheximide, a protein synthesis inhibitor, markedly sensitized cells to Fas-mediated apoptosis along with caspase 8 activation and c-FLIP(L) (cellular FLICE inhibitory protein long form) downregulation. ammonium ferrous sulfate 117-120 caspase 8 Homo sapiens 151-160 16179347-5 2005 In support of our contention that caspases play a functional role in the mature red cell, the oxidatively stressed red cell recapitulated apoptotic events, including translocation of Fas into rafts, formation of a Fas-associated complex, and activation of caspases 8 and 3. ammonium ferrous sulfate 183-186 caspase 8 Homo sapiens 34-42 16179347-5 2005 In support of our contention that caspases play a functional role in the mature red cell, the oxidatively stressed red cell recapitulated apoptotic events, including translocation of Fas into rafts, formation of a Fas-associated complex, and activation of caspases 8 and 3. ammonium ferrous sulfate 214-217 caspase 8 Homo sapiens 34-42 16289096-10 2005 In addition, caspase-8-deficient Jurkat T cells are resistant to Fas- and TNF-alpha-induced cell death. ammonium ferrous sulfate 65-68 caspase 8 Homo sapiens 13-22 15849201-11 2005 These studies thus define two distinct mechanisms for biologically relevant ASMase activation within rafts; a Fas-mediated mechanism dependent upon caspase 8 and FADD, and a UV-induced mechanism independent of caspase activation. ammonium ferrous sulfate 110-113 caspase 8 Homo sapiens 148-157 15677568-8 2005 These results extend the observation that Fas-dependent activation of caspase-8 accounts for apoptosis of early stage MDS erythroid cells and demonstrate for the first time that FADD is a valuable target to correct ineffective erythropoiesis in these syndromes. ammonium ferrous sulfate 42-45 caspase 8 Homo sapiens 70-79 15863130-0 2005 Sensitivity to Fas-mediated apoptosis in high-risk HPV-positive human cervical cancer cells: relationship with Fas, caspase-8, and Bid. ammonium ferrous sulfate 15-18 caspase 8 Homo sapiens 116-125 15863130-9 2005 Analysis of the Fas apoptotic pathway showed that anti-Fas treatment induced caspase-8 activation and concomitantly Bid cleavage, caspase-9 and caspase-3 activation, PARP cleavage and apoptosis in HeLa and CaSki. ammonium ferrous sulfate 55-58 caspase 8 Homo sapiens 77-86 15863130-12 2005 CONCLUSION: Sensitivity to anti-Fas depends on Fas, caspase-8, and Bid protein levels in cervical cancer cells. ammonium ferrous sulfate 32-35 caspase 8 Homo sapiens 52-61 15863130-9 2005 Analysis of the Fas apoptotic pathway showed that anti-Fas treatment induced caspase-8 activation and concomitantly Bid cleavage, caspase-9 and caspase-3 activation, PARP cleavage and apoptosis in HeLa and CaSki. ammonium ferrous sulfate 16-19 caspase 8 Homo sapiens 77-86 15750764-4 2005 Fas pathway analysis showed that Fas-resistant apoptosis of Fas-positive myeloma cells parallels FLIP (FLICE/caspase-8-inhibitory protein) expression. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 103-108 15750764-4 2005 Fas pathway analysis showed that Fas-resistant apoptosis of Fas-positive myeloma cells parallels FLIP (FLICE/caspase-8-inhibitory protein) expression. ammonium ferrous sulfate 33-36 caspase 8 Homo sapiens 103-108 15634909-4 2005 Importantly, our data indicate that the effect of wtp53 on the Fas-mediated pathway involves a degradation of short cellular FLICE inhibitory protein resulting in subsequent caspase 8 activation. ammonium ferrous sulfate 63-66 caspase 8 Homo sapiens 125-130 15634909-4 2005 Importantly, our data indicate that the effect of wtp53 on the Fas-mediated pathway involves a degradation of short cellular FLICE inhibitory protein resulting in subsequent caspase 8 activation. ammonium ferrous sulfate 63-66 caspase 8 Homo sapiens 174-183 15289496-2 2004 Here, we show that Fas activates NFkappaB via a pathway involving RIP, FADD, and caspase-8. ammonium ferrous sulfate 19-22 caspase 8 Homo sapiens 81-90 15390286-0 2004 Interferon-gamma sensitizes osteosarcoma cells to Fas-induced apoptosis by up-regulating Fas receptors and caspase-8. ammonium ferrous sulfate 50-53 caspase 8 Homo sapiens 107-116 15390286-9 2004 CONCLUSIONS: IFN-gamma sensitizes osteosarcoma cells to Fas-induced apoptosis through up-regulation of Fas receptor and caspase-8. ammonium ferrous sulfate 56-59 caspase 8 Homo sapiens 120-129 15726829-7 2004 A pan-caspase inhibitor, z-VAD, fully blocked cyt c release and oxidation of PtdSer in Fas-treated A549 cells. ammonium ferrous sulfate 87-90 caspase 8 Homo sapiens 6-13 15159409-5 2004 Fas-induced, caspase-8-independent exposure of the N terminus of Bak is blocked by staurosporine, a pan protein kinase inhibitor. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 13-22 15159409-6 2004 These results suggest that Fas stimulation not only activates caspase-8, but also a distinct signaling pathway involving protein kinase(s) to induce exposure of the N terminus of Bak. ammonium ferrous sulfate 27-30 caspase 8 Homo sapiens 62-71 14764700-7 2004 Fas immunoprecipitation reveals that caspase-3 is a component of the death-inducing signaling complex, suggesting that this cysteine protease is in close proximity to caspase-8. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 167-176 15013668-5 2004 We observed increased levels of several Fas-mediated apoptosis effectors (Fas, Fas ligand, FADD, FLICE), both in cell cultures at advanced passages and in skin cells of aged donors (above 45 years). ammonium ferrous sulfate 40-43 caspase 8 Homo sapiens 97-102 15279653-6 2004 Fas-mediated apoptosis of HSG cells was slightly inhibited by the caspase-9 inhibitor although it was mainly inhibited by that for caspase-8. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 131-140 12844494-5 2003 Moreover, NF-kappaB-dependent protection against Fas-mediated apoptosis was associated with decreased levels of caspase-8 activity and with the up-regulation of intracellular antiapoptotic proteins. ammonium ferrous sulfate 49-52 caspase 8 Homo sapiens 112-121 15163118-6 2004 After Fas triggering, the generation of the active sub-units of caspase-8 is significantly reduced in T1DM T-cells restimulated via TCR/CD3 and/or CD28. ammonium ferrous sulfate 6-9 caspase 8 Homo sapiens 64-73 14688367-11 2004 FLIP-caspase-8 balance seems tightly regulated in fibroblasts by extracellular factors that determine their susceptibility to Fas- or Fas-CHX-induced apoptosis. ammonium ferrous sulfate 126-129 caspase 8 Homo sapiens 5-14 14688367-11 2004 FLIP-caspase-8 balance seems tightly regulated in fibroblasts by extracellular factors that determine their susceptibility to Fas- or Fas-CHX-induced apoptosis. ammonium ferrous sulfate 134-137 caspase 8 Homo sapiens 5-14 14665704-8 2004 Using a XIAP inhibitor, phenoxodiol, we demonstrate that XIAP inactivation sensitizes trophoblast cells to Fas stimulation, as evidenced by the anti-Fas mAb-induced decrease in trophoblast cell viability and increase in caspase-8, caspase-9 and caspase-3 activation. ammonium ferrous sulfate 107-110 caspase 8 Homo sapiens 220-229 12957657-8 2003 Further analysis suggested a redox-sensitive step early in Fas signaling at the level of initiator caspase (caspase-8) activation. ammonium ferrous sulfate 59-62 caspase 8 Homo sapiens 108-117 12938225-3 2003 Furthermore, following overexpression of a human Fas:FLICE construct, which directly induces caspase activation in a death-inducing signaling complex-independent manner, cells could not be protected through BCR stimulation.Co-incubation with cycloheximide partially reversed protection from apoptosis and increased Fas-stimulated initiator and effector caspase activation, suggesting new protein synthesis is necessary to induce protection upstream of caspase activation. ammonium ferrous sulfate 49-52 caspase 8 Homo sapiens 53-58 12938225-2 2003 Using the B lymphoma line A20.2J, BCR signaling immediately inhibited Fas-induced apoptosis upstream of caspase-8 activation, as determined by Ile-Glu-Thr-Asp-(IETD)ase activity and cleavage of the caspase-8 substrate Bid. ammonium ferrous sulfate 70-73 caspase 8 Homo sapiens 104-113 12938225-2 2003 Using the B lymphoma line A20.2J, BCR signaling immediately inhibited Fas-induced apoptosis upstream of caspase-8 activation, as determined by Ile-Glu-Thr-Asp-(IETD)ase activity and cleavage of the caspase-8 substrate Bid. ammonium ferrous sulfate 70-73 caspase 8 Homo sapiens 198-207 12930371-9 2003 Analysis of Fas-induced activation of caspase-8 and -9 showed decreased activity of both caspases in HT, whereas activity of caspase-9 was increased and that of caspase-8 was decreased in GD. ammonium ferrous sulfate 12-15 caspase 8 Homo sapiens 38-54 12930371-9 2003 Analysis of Fas-induced activation of caspase-8 and -9 showed decreased activity of both caspases in HT, whereas activity of caspase-9 was increased and that of caspase-8 was decreased in GD. ammonium ferrous sulfate 12-15 caspase 8 Homo sapiens 89-97 12930371-9 2003 Analysis of Fas-induced activation of caspase-8 and -9 showed decreased activity of both caspases in HT, whereas activity of caspase-9 was increased and that of caspase-8 was decreased in GD. ammonium ferrous sulfate 12-15 caspase 8 Homo sapiens 38-47 12888916-4 2003 The enhancement of Fas-induced apoptosis by pre-treatment with neuraminidase was inhibited by z-VAD-fmk, a broad caspase inhibitor, and Ac-LEHD-CHO, an inhibitor of caspase-9, but not by Ac-IETD-CHO an inhibitor of caspase-8 or 6, imipramine, an inhibitor of acidic sphingomyelinase, glutathione, an inhibitor of neutral sphingomyelinase and Fumonisin B1, an inhibitor of ceramide synthase. ammonium ferrous sulfate 19-22 caspase 8 Homo sapiens 215-224 12804643-0 2003 Differential recruitment of caspase 8 to cFlip confers sensitivity or resistance to Fas-mediated apoptosis in a subset of familial lymphoma patients. ammonium ferrous sulfate 84-87 caspase 8 Homo sapiens 28-37 12804643-4 2003 Fas resistance in these cells was associated with reduced recruitment of the initiator caspase 8 compared to cFlip, an inhibitor of apoptosis, to the death-inducing signaling complex (DISC). ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 87-96 12841868-0 2003 Expression of short-form caspase 8 correlates with decreased sensitivity to Fas-mediated apoptosis in neuroblastoma cells. ammonium ferrous sulfate 76-79 caspase 8 Homo sapiens 25-34 12937219-13 2003 The inhibitors of either caspase-3 or caspase-8 could prevent the Fas-induced apoptosis in HPMCs. ammonium ferrous sulfate 66-69 caspase 8 Homo sapiens 38-47 12881712-5 2003 The expression of the death adapter FADD and caspase-8 was required for Fas-induced FOXO3a cleavage, but activation of survival pathways by overexpression of FLICE-inhibitory protein or phorbol myristate acetate treatment prevented it. ammonium ferrous sulfate 72-75 caspase 8 Homo sapiens 45-54 12841868-2 2003 Caspase 8, one of the initiator caspases, plays an important role in the Fas-Fas ligand pathway. ammonium ferrous sulfate 73-76 caspase 8 Homo sapiens 0-9 12841868-2 2003 Caspase 8, one of the initiator caspases, plays an important role in the Fas-Fas ligand pathway. ammonium ferrous sulfate 73-76 caspase 8 Homo sapiens 32-40 12841868-2 2003 Caspase 8, one of the initiator caspases, plays an important role in the Fas-Fas ligand pathway. ammonium ferrous sulfate 77-80 caspase 8 Homo sapiens 0-9 12841868-2 2003 Caspase 8, one of the initiator caspases, plays an important role in the Fas-Fas ligand pathway. ammonium ferrous sulfate 77-80 caspase 8 Homo sapiens 32-40 12841868-5 2003 Sensitivity to Fas-mediated cell death did not correlate with the expression of Fas in neuroblastoma cells, but was directly associated with the pattern of caspase 8 protein expression. ammonium ferrous sulfate 15-18 caspase 8 Homo sapiens 156-165 12841868-8 2003 More interestingly, a group of cell lines expressing a distinct short form of caspase 8 with splicing out of exon 3 consistently showed moderate sensitivity to Fas-mediated cell death. ammonium ferrous sulfate 160-163 caspase 8 Homo sapiens 78-87 12929750-5 2003 Inhibition of caspase-8 by Lle-Glu (OMe)-Thr-Asp(OMe)-fluoromethyl ketone completely blocked caspase-3 cleavage and apoptosis in UVA-treated cells, suggesting that apoptosis was initiated by the Fas pathway. ammonium ferrous sulfate 195-198 caspase 8 Homo sapiens 14-23 12738800-8 2003 Fas was also increased after TBI vs. control and was associated with relative levels of caspase-8, supporting formation of a death complex. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 88-97 12884866-3 2003 We previously reported that the prodomain-only isoforms of caspase-8 and -10 (PDCasp8/10), containing two DED motifs, could inhibit Fas-mediated apoptosis. ammonium ferrous sulfate 132-135 caspase 8 Homo sapiens 59-76 14532729-11 2003 PPAR-gamma agonist seems to enhance Fas-mediated apoptosis by affecting the way between caspase-8 and caspase-3. ammonium ferrous sulfate 36-39 caspase 8 Homo sapiens 88-97 12482880-1 2003 Upon engagement with Fas ligand (FasL), Fas rapidly induces recruitment and self-processing of caspase-8 via the adaptor protein Fas-associated death domain (FADD), and activated caspase-8 cleaves downstream substrates such as caspase-3. ammonium ferrous sulfate 21-24 caspase 8 Homo sapiens 95-104 12605597-4 2003 Indeed, using Jurkat cells as a model system, we demonstrate that NO donors block Fas- and etoposide-induced caspase activation and apoptosis (downstream of mitochondrial membrane depolarization) and cytochrome c release. ammonium ferrous sulfate 82-85 caspase 8 Homo sapiens 109-116 12551927-0 2003 Epoxycyclohexenone inhibits Fas-mediated apoptosis by blocking activation of pro-caspase-8 in the death-inducing signaling complex. ammonium ferrous sulfate 28-31 caspase 8 Homo sapiens 81-90 12496285-3 2003 Fas agonistic antibody, CH-11, induced apoptosis in sensitive glioma cells through caspase-8 recruitment to the Fas-mediated death-inducing signaling complex (DISC) where caspase-8 was cleaved to initiate apoptosis through a systematic cleavage of downstream substrates. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 83-92 12496285-3 2003 Fas agonistic antibody, CH-11, induced apoptosis in sensitive glioma cells through caspase-8 recruitment to the Fas-mediated death-inducing signaling complex (DISC) where caspase-8 was cleaved to initiate apoptosis through a systematic cleavage of downstream substrates. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 171-180 12496285-4 2003 In contrast, CH-11 stimulated cell growth in resistant glioma cells through recruitment of c-FLIP (cellular Fas-associated death domain (FADD)-like interleukin-1beta-converting enzyme (FLICE)-inhibitory protein) to the Fas-mediated DISC. ammonium ferrous sulfate 108-111 caspase 8 Homo sapiens 185-190 12393527-8 2003 FADD-like interleukin 1 beta (IL-1beta)-converting enzyme (FLICE)-inhibitory protein (FLIP) has been reported to interact with FADD and/or caspase-8 at the death-inducing signaling complex (DISC) level following Fas stimulation and acts as a dominant-negative caspase-8. ammonium ferrous sulfate 212-215 caspase 8 Homo sapiens 59-64 12393527-8 2003 FADD-like interleukin 1 beta (IL-1beta)-converting enzyme (FLICE)-inhibitory protein (FLIP) has been reported to interact with FADD and/or caspase-8 at the death-inducing signaling complex (DISC) level following Fas stimulation and acts as a dominant-negative caspase-8. ammonium ferrous sulfate 212-215 caspase 8 Homo sapiens 139-148 12393527-8 2003 FADD-like interleukin 1 beta (IL-1beta)-converting enzyme (FLICE)-inhibitory protein (FLIP) has been reported to interact with FADD and/or caspase-8 at the death-inducing signaling complex (DISC) level following Fas stimulation and acts as a dominant-negative caspase-8. ammonium ferrous sulfate 212-215 caspase 8 Homo sapiens 260-269 12702723-9 2003 FADD and caspase-8 colocalized with Fas in Jurkat cells validating the presence of FAF1 in the authentic Fas-DISC. ammonium ferrous sulfate 36-39 caspase 8 Homo sapiens 9-18 12702723-11 2003 In addition, the FAF1 deletion mutant lacking the N terminus where Fas, FADD, and caspase-8 interact protected Jurkat cells from Fas-induced apoptosis demonstrating dominant-negative phenotype. ammonium ferrous sulfate 129-132 caspase 8 Homo sapiens 82-91 12482880-1 2003 Upon engagement with Fas ligand (FasL), Fas rapidly induces recruitment and self-processing of caspase-8 via the adaptor protein Fas-associated death domain (FADD), and activated caspase-8 cleaves downstream substrates such as caspase-3. ammonium ferrous sulfate 21-24 caspase 8 Homo sapiens 179-188 12439910-5 2002 After the cells were transiently transfected with Fas and FADD antisense oligonucleotides, respectively, caspase-8 activity was determined by flurometric method. ammonium ferrous sulfate 50-53 caspase 8 Homo sapiens 105-114 12605685-2 2003 Recently, we reported that the caspase-resistant BAP31 inhibited Fas-mediated apoptotic membrane fragmentation and the release of cytochrome c from mitochondria in KB epithelial cells (Nguyen M., Breckenridge G., Ducret A & Shore G. (2000) Mol. ammonium ferrous sulfate 65-68 caspase 8 Homo sapiens 31-38 12566414-6 2003 A systematic examination of the downstream effectors of Fas signaling in IRF-4-transfected cells demonstrates an increased activation of caspase-8, as well as an increase in Fas receptor polarization. ammonium ferrous sulfate 56-59 caspase 8 Homo sapiens 137-146 12770553-7 2003 Hence, Fas appears to be functional through a caspase-8-dependent pathway in a subpopulation of human foetal motoneurons. ammonium ferrous sulfate 7-10 caspase 8 Homo sapiens 46-55 12633570-6 2002 Taken together, these findings suggest that IFN-gamma potentiates Fas- and TRAIL-mediated apoptosis by increasing caspase-8 expression via an IFN-gamma response element in A549 cells. ammonium ferrous sulfate 66-69 caspase 8 Homo sapiens 114-123 12415011-10 2002 Both forms of caspase-8-dependent cell death were also detected downstream of Fas in Jurkat T-cells, where Fas-dependent PS externalization and delayed ceramide production, which is similar to results shown here in A20 cells, have been reported. ammonium ferrous sulfate 78-81 caspase 8 Homo sapiens 14-23 12439910-10 2002 After SGC-7901 cells were transfected with Fas and FADD antisense oligonucleotides, caspase-8 activity was obviously decreased (P<0.01), whereas Fas blocked more than FADD. ammonium ferrous sulfate 43-46 caspase 8 Homo sapiens 84-93 12181741-0 2002 Mitomycin C induces apoptosis and caspase-8 and -9 processing through a caspase-3 and Fas-independent pathway. ammonium ferrous sulfate 86-89 caspase 8 Homo sapiens 34-50 12402161-6 2002 Furthermore, we have shown that inhibition of Fas mediated apoptosis is an early event in DU 145 cells, occurring upstream of Caspase 8 cleavage. ammonium ferrous sulfate 46-49 caspase 8 Homo sapiens 126-135 12404126-8 2002 These studies demonstrate that P-gp inhibits Fas-induced caspase-8 activation but not formation of the DISC and that this activity of P-gp is dependent on ATP hydrolysis. ammonium ferrous sulfate 45-48 caspase 8 Homo sapiens 57-66 12138895-0 2002 Possible attenuation of fas-mediated signaling by dominant expression of caspase-8 aberrant isoform in adult T-cell leukemia cells. ammonium ferrous sulfate 24-27 caspase 8 Homo sapiens 73-82 12432251-8 2002 In the Fas pathway, the anti-apoptotic activity of GAGE-7C maps downstream of caspase-8 activation and upstream of poly (ADP-ribose) polymerase (PARP) cleavage. ammonium ferrous sulfate 7-10 caspase 8 Homo sapiens 78-87 12097160-8 2002 Interestingly, a significant increase in the percentage of cells exhibiting caspase-8 and FLIP cleavage was observed upon Fas stimulation in interferon-gamma-treated U937 cells, in which the susceptibility to Fas is extremely enhanced. ammonium ferrous sulfate 122-125 caspase 8 Homo sapiens 76-85 12097160-11 2002 Using this system, we found that Fas-susceptibility changes during U937 differentiation occur upstream of caspase-8 processing/activation. ammonium ferrous sulfate 33-36 caspase 8 Homo sapiens 106-115 12037669-2 2002 In this study, to explore the possibility that the mutations of the genes involved in the proximal pathway of Fas-mediated apoptosis (Fas, FADD, caspase 8 and caspase 10) are involved in cancer metastasis, we have analysed somatic mutation and deletion of these genes in 80 non-small cell lung cancers (NSCLCs) with (n=43) and without (n=37) metastasis to the regional lymph nodes. ammonium ferrous sulfate 110-113 caspase 8 Homo sapiens 145-154 12010809-1 2002 Caspase-8 (Fas-associating protein with death domain-like interleukin-1beta- converting enzyme [FLICE]/MACH/Mch5) belongs to a family of cysteine proteases presumed to be the apex of the apoptotic signaling pathways. ammonium ferrous sulfate 11-14 caspase 8 Homo sapiens 0-9 12010809-1 2002 Caspase-8 (Fas-associating protein with death domain-like interleukin-1beta- converting enzyme [FLICE]/MACH/Mch5) belongs to a family of cysteine proteases presumed to be the apex of the apoptotic signaling pathways. ammonium ferrous sulfate 11-14 caspase 8 Homo sapiens 96-101 12010809-1 2002 Caspase-8 (Fas-associating protein with death domain-like interleukin-1beta- converting enzyme [FLICE]/MACH/Mch5) belongs to a family of cysteine proteases presumed to be the apex of the apoptotic signaling pathways. ammonium ferrous sulfate 11-14 caspase 8 Homo sapiens 108-112 12010809-8 2002 Moreover, Fas-mediated apoptosis was inhibited in caspase-8L-transfected Jurkat cells, which were associated with a reduction in the caspase-8 catalytic activity. ammonium ferrous sulfate 10-13 caspase 8 Homo sapiens 50-57 12010809-8 2002 Moreover, Fas-mediated apoptosis was inhibited in caspase-8L-transfected Jurkat cells, which were associated with a reduction in the caspase-8 catalytic activity. ammonium ferrous sulfate 10-13 caspase 8 Homo sapiens 50-59 12010809-9 2002 In vitro binding assays demonstrated that caspase-8L bound to FADD (Fas-associating protein with death domain) and caspase-8a and blocked the binding of caspase-8 to FADD. ammonium ferrous sulfate 68-71 caspase 8 Homo sapiens 42-51 11894131-1 2002 Fas engagement rapidly induces formation of the death-inducing signaling complex (DISC) that consists of Fas, FADD and pro-caspase-8. ammonium ferrous sulfate 0-3 caspase 8 Homo sapiens 123-132 11836578-3 2002 The participation of the Fas-mediated apoptotic pathway was suggested by the upregulation of the surface Fas molecule, the reduction of the apoptotic cell fraction after inhibition of caspase 8 which is a Fas-related initiator caspase, and the changes in Fas-related genes after irradiation. ammonium ferrous sulfate 25-28 caspase 8 Homo sapiens 184-193 11836578-3 2002 The participation of the Fas-mediated apoptotic pathway was suggested by the upregulation of the surface Fas molecule, the reduction of the apoptotic cell fraction after inhibition of caspase 8 which is a Fas-related initiator caspase, and the changes in Fas-related genes after irradiation. ammonium ferrous sulfate 105-108 caspase 8 Homo sapiens 184-193 11836578-3 2002 The participation of the Fas-mediated apoptotic pathway was suggested by the upregulation of the surface Fas molecule, the reduction of the apoptotic cell fraction after inhibition of caspase 8 which is a Fas-related initiator caspase, and the changes in Fas-related genes after irradiation. ammonium ferrous sulfate 105-108 caspase 8 Homo sapiens 184-193 11894131-12 2002 Altogether, the present data provide evidence that the Fas signal in AIDS-KS cells is preferentially transduced through the mitochondria-dependent pathway, which is initiated by caspase-8 activation. ammonium ferrous sulfate 55-58 caspase 8 Homo sapiens 178-187