PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 18339865-6 2008 Furthermore, we showed that GA inhibited the activations of vascular endothelial growth factor receptor 2 and its downstream protein kinases, such as c-Src, focal adhesion kinase, and AKT. gambogic acid 28-30 AKT serine/threonine kinase 1 Homo sapiens 184-187 18226852-7 2008 The results of Western blot and immunoprecipitation assay revealed that GA inhibits the phosphorylation of Akt. gambogic acid 72-74 AKT serine/threonine kinase 1 Homo sapiens 107-110 18226852-9 2008 These data suggested that GA inhibits the posttranslational modification of hTERT by inhibiting the phosphorylation of Akt. gambogic acid 26-28 AKT serine/threonine kinase 1 Homo sapiens 119-122 18226852-10 2008 Collectively, we suggest that GA represses telomerase activity not only by repressing hTERT transcriptional activity via c-Myc but also by posttranslational modification of hTERT via Akt. gambogic acid 30-32 AKT serine/threonine kinase 1 Homo sapiens 183-186 33971977-0 2021 Gambogic acid protects LPS-induced apoptosis and inflammation in a cell model of neonatal pneumonia through the regulation of TrkA/Akt signaling pathway. gambogic acid 0-13 AKT serine/threonine kinase 1 Homo sapiens 131-134 33971977-5 2021 In addition, human AKT serine/threonine kinase 1 (Akt) gene was knocked down in WI-38 cells to further investigate the associated genetic mechanisms of GA in protecting LPS-induced inflammation and apoptosis. gambogic acid 152-154 AKT serine/threonine kinase 1 Homo sapiens 19-48 33971977-5 2021 In addition, human AKT serine/threonine kinase 1 (Akt) gene was knocked down in WI-38 cells to further investigate the associated genetic mechanisms of GA in protecting LPS-induced inflammation and apoptosis. gambogic acid 152-154 AKT serine/threonine kinase 1 Homo sapiens 50-53 33971977-7 2021 Using western blot assay, it was demonstrated that GA promoted TrkA phosphorylation and Akt activation in LPS-treated WI-38 cells. gambogic acid 51-53 AKT serine/threonine kinase 1 Homo sapiens 88-91 33971977-8 2021 Knocking down Akt gene in WI-38 cells showed that GA-associated protections against LPS-induced apoptosis and inflammation were significantly reduced. gambogic acid 50-52 AKT serine/threonine kinase 1 Homo sapiens 14-17 33971977-9 2021 CONCLUSIONS: GA protected LPS-induced apoptosis and inflammation, possibly through the activations of TrkA and Akt signaling pathway. gambogic acid 13-15 AKT serine/threonine kinase 1 Homo sapiens 111-114 32913452-0 2020 Gambogic acid affects ESCC progression through regulation of PI3K/AKT/mTOR signal pathway. gambogic acid 0-13 AKT serine/threonine kinase 1 Homo sapiens 66-69 32913452-5 2020 Further investigation showed that GA down-regulated the levels of PI3K, p-AKT and p-mTOR, while promoted PTEN expression in ESCC cells. gambogic acid 34-36 AKT serine/threonine kinase 1 Homo sapiens 74-77 32913452-6 2020 Taken together, we provided the first demonstration that GA exerts anti-tumor effects on ESCC cells presumably through regulating PTEN-PI3K-AKT-mTORpathway, suggestive of a therapeutic potential for ESCC. gambogic acid 57-59 AKT serine/threonine kinase 1 Homo sapiens 140-143 31748028-10 2020 These results suggested that downregulation of PI3K/AKT/mTOR signaling pathway may be an important mediator in GA-affected ribosomal occurrence in glioma cells. gambogic acid 111-113 AKT serine/threonine kinase 1 Homo sapiens 52-55 31748028-3 2020 In the current study, the effect of GA on phosphoinositide kinase-3 (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway was examined in human U251 glioma cells. gambogic acid 36-38 AKT serine/threonine kinase 1 Homo sapiens 93-96 31748028-7 2020 GA decreased the phosphorylation of P38, AKT, and mTOR. gambogic acid 0-2 AKT serine/threonine kinase 1 Homo sapiens 41-44 31555380-7 2019 Compared with the NC group, the expression levels of PI3K, AKT, phosphorylated-AKT, P21 and MMP-2 and -9 were significantly altered in a dose dependent manner following treatment with GA (all P<0.05). gambogic acid 184-186 AKT serine/threonine kinase 1 Homo sapiens 59-62 31555380-7 2019 Compared with the NC group, the expression levels of PI3K, AKT, phosphorylated-AKT, P21 and MMP-2 and -9 were significantly altered in a dose dependent manner following treatment with GA (all P<0.05). gambogic acid 184-186 AKT serine/threonine kinase 1 Homo sapiens 79-82 31555380-8 2019 The results of the current study indicated that GA suppressed proliferation and dispersion of human colon cancer cells in a dose-dependent manner, possibly through a PI3K/AKT/P21/MMP-2/9-dependent pathway. gambogic acid 48-50 AKT serine/threonine kinase 1 Homo sapiens 171-174 29050671-6 2017 Western blot analysis showed that GA treatment suppressed the activation of Akt, mTOR, and S6. gambogic acid 34-36 AKT serine/threonine kinase 1 Homo sapiens 76-79 30269846-0 2018 Gambogic acid induces cell apoptosis through endoplasmic reticulum stress triggered inhibition of Akt signaling pathways in extranodal NK/T-cell lymphoma cells. gambogic acid 0-13 AKT serine/threonine kinase 1 Homo sapiens 98-101 30269846-4 2018 Furthermore, GA induced cell death through endoplasmic reticulum stress (ERS) mediated suppression of Akt signaling pathways and finally the release of the caspase-3 proteases. gambogic acid 13-15 AKT serine/threonine kinase 1 Homo sapiens 102-105 30186399-8 2018 Additionally, western blot analysis demonstrated that GA treatment enhanced the activation of phosphatase and tensin homolog (PTEN) along with the suppression of PI3K and p-Akt. gambogic acid 54-56 AKT serine/threonine kinase 1 Homo sapiens 173-176 26318432-0 2016 Gambogic acid enhances the radiosensitivity of human esophageal cancer cells by inducing reactive oxygen species via targeting Akt/mTOR pathway. gambogic acid 0-13 AKT serine/threonine kinase 1 Homo sapiens 127-130 28901512-7 2017 Treatment with gambogic acid suppressed the activities of interleukin (IL)-1beta and IL-6, promoted the protein expression of phosphorylated (p)-Akt serine/threonine kinase (Akt), p-mammalian target protein of rapamycin (mTOR) and inhibited hypoxia-inducible factor-1alpha and vascular endothelial growth factor expression in RA rats. gambogic acid 15-28 AKT serine/threonine kinase 1 Homo sapiens 145-148 28901512-7 2017 Treatment with gambogic acid suppressed the activities of interleukin (IL)-1beta and IL-6, promoted the protein expression of phosphorylated (p)-Akt serine/threonine kinase (Akt), p-mammalian target protein of rapamycin (mTOR) and inhibited hypoxia-inducible factor-1alpha and vascular endothelial growth factor expression in RA rats. gambogic acid 15-28 AKT serine/threonine kinase 1 Homo sapiens 174-177 27284372-6 2016 The inhibition of protein kinase B (AKT) and NF-kappaB activation by chemical inhibitors augmented the apoptotic effect responses to GA in the TE-1 cells. gambogic acid 133-135 AKT serine/threonine kinase 1 Homo sapiens 36-39 26205493-0 2015 Gambogic acid inhibits osteoclast formation and ovariectomy-induced osteoporosis by suppressing the JNK, p38 and Akt signalling pathways. gambogic acid 0-13 AKT serine/threonine kinase 1 Homo sapiens 113-116 24810758-5 2014 Functional studies revealed that GA-induced dysregulation of lipid metabolism could activate 5-lipoxygenase (5-LOX), resulting in intracellular ROS accumulation, followed by inhibition of Akt-mTOR signaling and autophagy initiation. gambogic acid 33-35 AKT serine/threonine kinase 1 Homo sapiens 188-191 24890366-0 2014 Gambogic acid suppresses hypoxia-induced hypoxia-inducible factor-1alpha/vascular endothelial growth factor expression via inhibiting phosphatidylinositol 3-kinase/Akt/mammalian target protein of rapamycin pathway in multiple myeloma cells. gambogic acid 0-13 AKT serine/threonine kinase 1 Homo sapiens 164-167 24890366-7 2014 Mechanistic studies exhibited that GA inhibited the production of HIF-1alpha by reducing phosphorylation of Akt and mTOR in U266 cells. gambogic acid 35-37 AKT serine/threonine kinase 1 Homo sapiens 108-111 24890366-9 2014 Taken together, our results identify that GA suppresses hypoxia-activated pathways that are linked to MM progression, at least partly, by the inhibition of the PI3K/Akt/mTOR signaling pathway. gambogic acid 42-44 AKT serine/threonine kinase 1 Homo sapiens 165-168 23665322-0 2013 Gambogic acid induces EGFR degradation and Akt/mTORC1 inhibition through AMPK dependent-LRIG1 upregulation in cultured U87 glioma cells. gambogic acid 0-13 AKT serine/threonine kinase 1 Homo sapiens 43-46 23665322-5 2013 For mechanism study, we found that gambogic acid induced LRIG1 (leucine-rich repeat and Ig-like domain-containing-1) upregulation, which was responsible for EGFR (epidermal growth factor receptor) degradation and its downstream Akt/mTORC1 inhibition. gambogic acid 35-48 AKT serine/threonine kinase 1 Homo sapiens 228-231 23665322-3 2013 In the current study, we found that gambogic acid induced growth inhibition and apoptosis in cultured U87 glioma cells, which was associated with Akt/mTORC1 (mTOR complex 1) signaling in-activation. gambogic acid 36-49 AKT serine/threonine kinase 1 Homo sapiens 146-149 23665322-6 2013 Further, we provided evidence to support that AMPK (AMP-activated protein kinase) activation mediated gambogic acid-induced LRIG1 upregulation, U87 cell apoptosis and growth inhibition, while AMPK inhibition by shRNA or compound C reduced gambogic acid-induced EGFR/Akt inhibition and cytotoxicity in U87 cells. gambogic acid 102-115 AKT serine/threonine kinase 1 Homo sapiens 266-269 23665322-4 2013 To restore Akt activation by introducing a constitutively active (CA) Akt attenuated gambogic acid-induced cytotoxicity against U87 cells. gambogic acid 85-98 AKT serine/threonine kinase 1 Homo sapiens 11-14 23665322-4 2013 To restore Akt activation by introducing a constitutively active (CA) Akt attenuated gambogic acid-induced cytotoxicity against U87 cells. gambogic acid 85-98 AKT serine/threonine kinase 1 Homo sapiens 70-73 23194187-7 2012 GA also inhibited cell survival via blocking Akt/mTOR signaling. gambogic acid 0-2 AKT serine/threonine kinase 1 Homo sapiens 45-48 19433130-0 2009 Gambogic acid induces G0/G1 arrest and apoptosis involving inhibition of SRC-3 and inactivation of Akt pathway in K562 leukemia cells. gambogic acid 0-13 AKT serine/threonine kinase 1 Homo sapiens 99-102 22426696-0 2012 Gambogic acid inhibits TNF-alpha-induced invasion of human prostate cancer PC3 cells in vitro through PI3K/Akt and NF-kappaB signaling pathways. gambogic acid 0-13 AKT serine/threonine kinase 1 Homo sapiens 107-110 22426696-8 2012 Moreover, the TNF-alpha-mediated activation of phosphatidylinositol-3-OH kinase/protein kinase B (PI3K/Akt) and NF-kappaB pathways was suppressed by GA (0.5 mumol/L). gambogic acid 149-151 AKT serine/threonine kinase 1 Homo sapiens 103-106 22426696-11 2012 CONCLUSION: GA inhibits TNF-alpha-induced invasion of PC3 cells via inactivation of the PI3K/Akt and NF-kappaB signaling pathways, which may offer a novel approach for the treatment of human prostate cancer. gambogic acid 12-14 AKT serine/threonine kinase 1 Homo sapiens 93-96 22888247-0 2012 Synergistic effect of a combination of nanoparticulate Fe3O4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells. gambogic acid 65-78 AKT serine/threonine kinase 1 Homo sapiens 112-115 22888247-7 2012 Transcription of cytochrome c, caspase 9, and caspase 3 genes was higher in the group treated with magnetic nanoparticles containing Fe(3)O(4) and gambogic acid than in the groups that received gambogic acid or magnetic nanoparticles containing Fe(3)O(4), but transcription of phosphatidylinositol 3-kinase, Akt, and Bad genes decreased. gambogic acid 147-160 AKT serine/threonine kinase 1 Homo sapiens 308-311 22888247-8 2012 Notably, expression of cytochrome c, caspase 9, and caspase 3 proteins in the group treated with gambogic acid and magnetic nanoparticles containing Fe(3)O(4) was higher than in the groups receiving magnetic nanoparticles containing Fe(3)O(4) or gambogic acid, while expression of p-PI3K, p-Akt, p-Bad, pro-caspase 9, and pro-caspase 3 degraded. gambogic acid 97-110 AKT serine/threonine kinase 1 Homo sapiens 291-294 22888247-9 2012 CONCLUSION: Magnetic nanoparticles containing Fe(3)O(4) can enhance apoptosis induced by gambogic acid which may be closely related to regulation of the PI3K/Akt/Bad pathway in the treatment of human colon cancer. gambogic acid 89-102 AKT serine/threonine kinase 1 Homo sapiens 158-161 19433130-5 2009 GA treatment downregulated the expression of SRC-3 and then inhibited the activity of Akt kinase and its downstream targets p70 S6 kinase 1 (S6K1) and glycogen synthase kinase 3beta (GSK3beta) without changes in total protein levels of these three proteins, which thus influenced the expression of the apoptosis related gene Bcl-2 in K562 cells. gambogic acid 0-2 AKT serine/threonine kinase 1 Homo sapiens 86-89