PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15531381-9 2004 The calcein-AM test in untreated cells showed that GFJ, kaempferol or naringenin inhibited Pgp activity. calcein AM 4-14 phosphoglycolate phosphatase Homo sapiens 91-94 19879740-7 2010 However, calcein-AM uptake into the human P-gp overexpression cell line, LLC-GA5-COL300, was increased by curcumin and demethoxycurcumin in a concentration-dependent manner but not affected by bisdemethoxycurcumin. calcein AM 9-19 phosphoglycolate phosphatase Homo sapiens 42-46 12421806-9 2003 Substrates that stimulated the ATPase activity of P-gp (calcein-AM, demecolcine, cis(Z)-flupentixol, and verapamil) increased the rate of cross-linking between Cys(431)(NBD1-Walker A)/C1176C(NBD2-LSGGQ) and between Cys(1074)(NBD2-Walker A)/L531C(NBD1-LSGGQ) when compared with cross-linking in the absence of drug substrate. calcein AM 56-66 phosphoglycolate phosphatase Homo sapiens 50-54 12909621-5 2003 TMEA is a cross-linker substrate of P-gp that allowed us to test for stimulation of cross-linking by a second substrate such as calcein-acetoxymethyl ester, colchicine, demecolcine, cyclosporin A, rhodamine B, progesterone, and verapamil. calcein AM 128-155 phosphoglycolate phosphatase Homo sapiens 36-40 10419897-4 1999 However, a strong correlation was observed between the simultaneous activity of MRP1 and Pgp (quantified as the modulation of calcein-AM uptake by cyclosporin A and probenecid) and the LC50 of DNR (r =.77, P <.0001). calcein AM 126-136 phosphoglycolate phosphatase Homo sapiens 89-92 11856485-4 2002 The K(i) values for P-gp of cyclosporine and verapamil were 1.09 and 540 microM, respectively, and that of bromocriptine was 6.52 microM in a calcein-AM efflux assay using porcine kidney epithelial LLC-PK1 and L-MDR1 cells, overexpressing human P-gp. calcein AM 142-152 phosphoglycolate phosphatase Homo sapiens 20-24 11856485-4 2002 The K(i) values for P-gp of cyclosporine and verapamil were 1.09 and 540 microM, respectively, and that of bromocriptine was 6.52 microM in a calcein-AM efflux assay using porcine kidney epithelial LLC-PK1 and L-MDR1 cells, overexpressing human P-gp. calcein AM 142-152 phosphoglycolate phosphatase Homo sapiens 245-249 10891114-2 2000 In a standardized assay that measures Pgp function by the Pgp-mediated efflux of the calcein-AM Pgp substrate and uses human lymphoblastoid MDR-CEM (VBL(100)) cells as highly resistant Pgp-expressing cells and the cyclic undecapeptide cyclosporin A (CsA) as a reference MDR-reversing agent (IC(50) of 3.4 microM), AbA was found to be a more active Pgp inhibitor (IC(50) of 2.3 microM). calcein AM 85-95 phosphoglycolate phosphatase Homo sapiens 38-41 10891114-2 2000 In a standardized assay that measures Pgp function by the Pgp-mediated efflux of the calcein-AM Pgp substrate and uses human lymphoblastoid MDR-CEM (VBL(100)) cells as highly resistant Pgp-expressing cells and the cyclic undecapeptide cyclosporin A (CsA) as a reference MDR-reversing agent (IC(50) of 3.4 microM), AbA was found to be a more active Pgp inhibitor (IC(50) of 2.3 microM). calcein AM 85-95 phosphoglycolate phosphatase Homo sapiens 58-61 10891114-2 2000 In a standardized assay that measures Pgp function by the Pgp-mediated efflux of the calcein-AM Pgp substrate and uses human lymphoblastoid MDR-CEM (VBL(100)) cells as highly resistant Pgp-expressing cells and the cyclic undecapeptide cyclosporin A (CsA) as a reference MDR-reversing agent (IC(50) of 3.4 microM), AbA was found to be a more active Pgp inhibitor (IC(50) of 2.3 microM). calcein AM 85-95 phosphoglycolate phosphatase Homo sapiens 58-61 10891114-2 2000 In a standardized assay that measures Pgp function by the Pgp-mediated efflux of the calcein-AM Pgp substrate and uses human lymphoblastoid MDR-CEM (VBL(100)) cells as highly resistant Pgp-expressing cells and the cyclic undecapeptide cyclosporin A (CsA) as a reference MDR-reversing agent (IC(50) of 3.4 microM), AbA was found to be a more active Pgp inhibitor (IC(50) of 2.3 microM). calcein AM 85-95 phosphoglycolate phosphatase Homo sapiens 58-61 10891114-2 2000 In a standardized assay that measures Pgp function by the Pgp-mediated efflux of the calcein-AM Pgp substrate and uses human lymphoblastoid MDR-CEM (VBL(100)) cells as highly resistant Pgp-expressing cells and the cyclic undecapeptide cyclosporin A (CsA) as a reference MDR-reversing agent (IC(50) of 3.4 microM), AbA was found to be a more active Pgp inhibitor (IC(50) of 2.3 microM). calcein AM 85-95 phosphoglycolate phosphatase Homo sapiens 58-61 23879966-8 2013 VPA17 cells had increased Pgp-mediated efflux of calcein acetoxymethyl ester (calcein AM); however, this was inhibited in cells pre-incubated in silibinin for 5 days. calcein AM 49-76 phosphoglycolate phosphatase Homo sapiens 26-29 10500791-8 1999 These results suggest that functional testing (with calcein-AM +/- modulators) for the presence of both MRP1 and Pgp activities is of prognostic value and that MRP1 contributes to drug resistance in AML. calcein AM 52-62 phosphoglycolate phosphatase Homo sapiens 113-116 28899501-10 2017 The P-gp functional studies showed that these three alkaloids increased the accumulation of P-gp substrates, calcein-AM (C-AM) and rhodamine123 (Rho 123) in K562/Adr cells, while this effect was not seen in drug sensitive parental K562 cells. calcein AM 109-119 phosphoglycolate phosphatase Homo sapiens 4-8 28899501-10 2017 The P-gp functional studies showed that these three alkaloids increased the accumulation of P-gp substrates, calcein-AM (C-AM) and rhodamine123 (Rho 123) in K562/Adr cells, while this effect was not seen in drug sensitive parental K562 cells. calcein AM 121-125 phosphoglycolate phosphatase Homo sapiens 4-8 27154960-6 2016 Furthermore, we show functionality of translated P-gp protein in recipient cells using Calcein-AM dye exclusion assays on flow cytometry. calcein AM 87-97 phosphoglycolate phosphatase Homo sapiens 49-53 26689174-3 2016 The accumulation of P-gp substrates, Calcein-AM, Rhodamine 123 and Doxorubicin, was significantly increased by 1J (up to 6-, 11- and 22- folds, respectively) and 2J (up to 7-, 12- and 17- folds, respectively). calcein AM 37-47 phosphoglycolate phosphatase Homo sapiens 20-24 10500791-0 1999 Both Pgp and MRP1 activities using calcein-AM contribute to drug resistance in AML. calcein AM 35-45 phosphoglycolate phosphatase Homo sapiens 5-8 9616142-0 1998 Pgp and MRP activities using calcein-AM are prognostic factors in adult acute myeloid leukemia patients. calcein AM 29-39 phosphoglycolate phosphatase Homo sapiens 0-3 9616142-8 1998 These results suggest that functional testing (with calcein-AM +/- modulators) for the presence of both MRP and Pgp activities is of prognostic value and that MRP contributes to drug resistance in AML. calcein AM 52-62 phosphoglycolate phosphatase Homo sapiens 112-115 30466981-16 2018 Furthermore, Rhodamine 123 and Calcein-AM were used to detect the effects of polyphenols on the activity of P-gp. calcein AM 31-41 phosphoglycolate phosphatase Homo sapiens 108-112 27131064-4 2016 Among these derivatives, 5a bearing on the 3-methylphenyl substituent, displayed the most potent P-gp inhibitory activity, which can enable the increase of the intracellular accumulation of P-gp substrate Calcein-AM. calcein AM 205-215 phosphoglycolate phosphatase Homo sapiens 97-101 27131064-4 2016 Among these derivatives, 5a bearing on the 3-methylphenyl substituent, displayed the most potent P-gp inhibitory activity, which can enable the increase of the intracellular accumulation of P-gp substrate Calcein-AM. calcein AM 205-215 phosphoglycolate phosphatase Homo sapiens 190-194 26070251-10 2015 This increased expression resulted also in an increased functional transport activity as measured by the P-gp mediated efflux of calcein AM. calcein AM 129-139 phosphoglycolate phosphatase Homo sapiens 105-109 23999162-4 2013 Selected SM increased the accumulation of the rhodamine 123 (Rho123) and calcein-AM (CAM) in a dose dependent manner in Caco-2 cells, indicating that they act as competitive inhibitors of P-gp. calcein AM 85-88 phosphoglycolate phosphatase Homo sapiens 188-192 23879966-8 2013 VPA17 cells had increased Pgp-mediated efflux of calcein acetoxymethyl ester (calcein AM); however, this was inhibited in cells pre-incubated in silibinin for 5 days. calcein AM 78-88 phosphoglycolate phosphatase Homo sapiens 26-29 21414769-6 2012 A flow cytometry assay indicated that kuguacin J inhibits the transport function of P-gp and thereby significantly increases the accumulation of rhodamine 123 and calcein AM in the cells. calcein AM 163-173 phosphoglycolate phosphatase Homo sapiens 84-88 21864659-4 2011 Cellular accumulation of calcein-AM was further determined to confirm the affinity of gemifloxacin towards P-gp and MRP2. calcein AM 25-35 phosphoglycolate phosphatase Homo sapiens 107-111 22632934-3 2012 Calcein-AM substrate accumulation assays were performed in an MCF7/DX1 cell line that overexpresses P-gp to monitor the inhibitory activity of the clicked quinine dimers. calcein AM 0-10 phosphoglycolate phosphatase Homo sapiens 100-104