PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9553146-9 1998 We also show here that the inhibition of ERK triggers the caspase system, which is abolished by serum in a wortmannin-dependent manner. Wortmannin 107-117 mitogen-activated protein kinase 1 Homo sapiens 41-44 9720763-4 1998 We report that inhibition of PI3K by wortmannin or an inhibitory p85 construct, p85deltaiSH2, reduced the TCR-induced Syk-dependent activation of Erk2, as well as the appearance of phospho-Erk and phospho-Mek. Wortmannin 37-47 mitogen-activated protein kinase 1 Homo sapiens 146-150 9531494-7 1998 CPPD crystals also activated the ERK1 and ERK2 forms of MAP kinase (wortmannin insensitive), PKC (Compound 3 sensitive) and protein kinase B (wortmannin sensitive) in neutrophils. Wortmannin 68-78 mitogen-activated protein kinase 1 Homo sapiens 42-46 9766635-7 1998 The phosphatidylinositol 3-kinase inhibitor, wortmannin, attenuated stimulation of ERKs and p38 MAPK by GM-CSF, but not TNF-alpha. Wortmannin 45-55 mitogen-activated protein kinase 1 Homo sapiens 83-87 9766635-7 1998 The phosphatidylinositol 3-kinase inhibitor, wortmannin, attenuated stimulation of ERKs and p38 MAPK by GM-CSF, but not TNF-alpha. Wortmannin 45-55 mitogen-activated protein kinase 1 Homo sapiens 92-95 9234699-6 1997 Interestingly, integrin-mediated Erk-2, Mek-1, and Raf-1 activation, but not Ras-GTP loading, was inhibited at least 80% by wortmannin and LY294002. Wortmannin 124-134 mitogen-activated protein kinase 1 Homo sapiens 33-38 9572301-8 1998 In the case of Erk, activation was partially blocked by wortmannin or LY294002, indicating a possible link with PI 3-kinase. Wortmannin 56-66 mitogen-activated protein kinase 1 Homo sapiens 15-18 9469452-8 1998 However, wortmannin inhibited FMLP stimulation of neutrophil Erk only at concentrations (IC50 > or = 1 microM) inconsistent with an effect on PI 3-K. Our data indicate that PI 3-K mediates neutrophil adhesion by a mechanism independent of CD11b/CD18 up-regulation, suggesting that PI 3-K acts either parallel to, or downstream of, Erk. Wortmannin 9-19 mitogen-activated protein kinase 1 Homo sapiens 61-64 9469452-8 1998 However, wortmannin inhibited FMLP stimulation of neutrophil Erk only at concentrations (IC50 > or = 1 microM) inconsistent with an effect on PI 3-K. Our data indicate that PI 3-K mediates neutrophil adhesion by a mechanism independent of CD11b/CD18 up-regulation, suggesting that PI 3-K acts either parallel to, or downstream of, Erk. Wortmannin 9-19 mitogen-activated protein kinase 1 Homo sapiens 334-337 26923291-9 2016 Treatment of HUVECs with PD98059, SB203580 and Wortmannin, which are the inhibitors of ERK, P38 MAPK and AKT respectively, resulted in decreased SIRT1 expression and reduced migration of HUVEC cells. Wortmannin 47-57 mitogen-activated protein kinase 1 Homo sapiens 87-90 9162091-2 1997 We have previously shown that in T cells the PI3-K inhibitor, wortmannin, interferes with activation of the mitogen-activated kinase, Erk2, after T cell receptor (TcR) stimulation. Wortmannin 62-72 mitogen-activated protein kinase 1 Homo sapiens 134-138 9162091-9 1997 Wortmannin did not interfere with NFAT activation, although it inhibited PI3-K and Erk2 activation in the same experiment. Wortmannin 0-10 mitogen-activated protein kinase 1 Homo sapiens 83-87 8654435-3 1996 We report that treatment of cells with wortmannin blocked anti-CD3-induced activation of the mitogen-activation kinase Erk2 while not affecting phorbol-ester-induced Erk2 activation. Wortmannin 39-49 mitogen-activated protein kinase 1 Homo sapiens 119-123 8798386-6 1996 As expected for a Gi-coupled receptor, the inhibitors of phosphatidylinositol 3-kinase wortmannin and LY294002 inhibited activation of ERK2, albeit only partly (70%). Wortmannin 87-97 mitogen-activated protein kinase 1 Homo sapiens 135-139 7498528-2 1995 We studied the effects of wortmannin, a selective inhibitor of PI 3-K, on the activation of extracellular-signal regulated kinase (ERK) by PDGF in cultured hepatic stellate cells, mesenchymal cells responsible for extracellular matrix synthesis within the liver. Wortmannin 26-36 mitogen-activated protein kinase 1 Homo sapiens 92-129 7498528-2 1995 We studied the effects of wortmannin, a selective inhibitor of PI 3-K, on the activation of extracellular-signal regulated kinase (ERK) by PDGF in cultured hepatic stellate cells, mesenchymal cells responsible for extracellular matrix synthesis within the liver. Wortmannin 26-36 mitogen-activated protein kinase 1 Homo sapiens 131-134 7498528-3 1995 Incubation with 100 nM wortmannin, a dose which almost completely blocks PI 3-K, resulted in 50% reduction of ERK activity. Wortmannin 23-33 mitogen-activated protein kinase 1 Homo sapiens 110-113 7498528-4 1995 Direct inhibition of ERK by wortmannin could not be considered responsible for this effect, since wortmannin did not inhibit ERK activity in vitro. Wortmannin 28-38 mitogen-activated protein kinase 1 Homo sapiens 21-24 7498528-5 1995 Rather, inhibition of PI 3-K acts on the kinase cascade that leads to ERK activation, since PDGF-dependent phosphorylation of ERK was found to be reduced after incubation with wortmannin. Wortmannin 176-186 mitogen-activated protein kinase 1 Homo sapiens 70-73 7498528-5 1995 Rather, inhibition of PI 3-K acts on the kinase cascade that leads to ERK activation, since PDGF-dependent phosphorylation of ERK was found to be reduced after incubation with wortmannin. Wortmannin 176-186 mitogen-activated protein kinase 1 Homo sapiens 126-129 7498528-6 1995 Wortmannin also inhibited the increase in c-fos mRNA induced by PDGF, which is dependent on ERK activation. Wortmannin 0-10 mitogen-activated protein kinase 1 Homo sapiens 92-95 28395342-6 2017 In addition, FGF2 increases phosphorylation of AKT, P70S6K, S6, ERK1/2, JNK, P38, and P90RSK in a time-dependent manner, and increases in their expression was suppressed by Wortmannin (a phosphatidylinositol 3-kinase [PI3K] inhibitor), U0126 (an ERK1/2 inhibitor), SP600125 (a JNK inhibitor), and SB203580 (a P38 inhibitor) based on western blot analyses. Wortmannin 173-183 mitogen-activated protein kinase 1 Homo sapiens 309-312 27456341-5 2016 Concentration gradients of wortmannin and PD98095 were used to inhibit AKT and ERK expression, respectively in the NSCLC NCI-H1650-b and SCLC NCI-H446-b cell lines, and cell growth curves were drawn. Wortmannin 27-37 mitogen-activated protein kinase 1 Homo sapiens 79-82 26657209-8 2016 TGF-beta1 markedly stimulated the URG-4/URGCP mRNA and protein that was blocked by MEK1 [MAPK (Mitogen-Activated Protein Kinase)/ERK (extracellular signal-regulated kinase) kinase 1] inhibitor, PD98059 and PI3K inhibitor, wortmannin. Wortmannin 222-232 mitogen-activated protein kinase 1 Homo sapiens 129-132 24450273-6 2014 Inhibition of the ERK and Akt pathways using specific inhibitors (Wortmanin & PD98509) led to the activation of the p38 pathway. Wortmannin 66-75 mitogen-activated protein kinase 1 Homo sapiens 18-21 25858425-7 2015 Phosphorylation of Akt and phosphorylation of ERK were blocked by the phosphoinositide 3-kinase (PI3K) inhibitor wortmannin and the protein kinase C (PKC) inhibitor Ro31-8220, respectively, whereas Syk phosphorylation was not altered. Wortmannin 113-123 mitogen-activated protein kinase 1 Homo sapiens 46-49 23300886-7 2012 Pharmacological inhibition of JAK/STAT3, PI3K/Akt or MEK/ERK signaling by AG490, Wortmannin or U0126, respectively, reduced leptin-induced cyclin D1 expression and NP cell proliferation. Wortmannin 81-91 mitogen-activated protein kinase 1 Homo sapiens 57-60 22879882-8 2012 Furthermore, pre-treatment with PI3K inhibitor wortmannin or LY294002 prevented ephrinB1-Fc-induced ERK activation in spinal. Wortmannin 47-57 mitogen-activated protein kinase 1 Homo sapiens 100-103 21358682-7 2012 PI3K and p-ERK expression were also decreased in G-CSF-treated NK-92MI cells, and their inhibitors, wortmannin and PD98059, respectively, both enhanced the downregulation of cytotoxicity. Wortmannin 100-110 mitogen-activated protein kinase 1 Homo sapiens 11-14 22077956-11 2011 Furthermore, treatment with inhibitors of activation of ERK (PD098059) and Akt (wortmannin) leads to radiosensitization of MO59J spheroids. Wortmannin 80-90 mitogen-activated protein kinase 1 Homo sapiens 56-59 20471474-2 2010 In contrast to MCF7 cells, ERK phosphorylation in T47D cells displayed resistance to MEK inhibition by several structurally different compounds, such as U0126, PD 098059 and PD 198306, MEK suppression by small interfering RNA (siRNA) and was also less sensitive to PI3K inhibition by wortmannin. Wortmannin 284-294 mitogen-activated protein kinase 1 Homo sapiens 27-30 18256541-6 2008 Inhibition of PI3K with either LY294002 and wortmannin was sufficient to cause upregulation of ERK activity as measured by immunoblotting. Wortmannin 44-54 mitogen-activated protein kinase 1 Homo sapiens 95-98 20512938-3 2010 Further analyses made on HuVEC showed that the ERK inhibitor PD98059 maximally inhibited the PL-induced endothelial repair, followed in order of importance by the calcium chelator BAPTA-AM, the PI3K inhibitor wortmannin and the p38 inhibitor SB203580. Wortmannin 209-219 mitogen-activated protein kinase 1 Homo sapiens 47-50 19906641-8 2010 The 30-kDa protein was identified as ribosomal protein S6, a translational regulator, and its phosphorylation was inhibited by a phosphatidylinositol 3-kinase (PI3K) inhibitor (wortmannin) and an ERK inhibitor (PD98059) but not by a p38-MAPK inhibitor (SB203580). Wortmannin 177-187 mitogen-activated protein kinase 1 Homo sapiens 196-199 19917268-4 2010 The MEK inhibitor PD98059 and the PI3K inhibitor Wortmannin specifically inhibited sesamin-induced activation of the ERK and Akt/eNOS pathways. Wortmannin 49-59 mitogen-activated protein kinase 1 Homo sapiens 117-120 20008963-8 2010 Further study revealed that both MDG-1 and S1P induce Akt and ERK phosphorylation in a dose- and time-dependent manner, an effect that is attenuated by pre-treatment with either the Akt inhibitor wortmannin or the ERK inhibitor PD98059, and MDG-1 can also induce eNOS phosphorylation and increases in production of NO. Wortmannin 196-206 mitogen-activated protein kinase 1 Homo sapiens 62-65 20402656-10 2010 ERK activation was inhibited by PD98059, Wortmanin and the ROS scavenger NAC (N-acetyl cysteine). Wortmannin 41-50 mitogen-activated protein kinase 1 Homo sapiens 0-3 19800362-6 2010 Rapamycin had no effect on the rejection of the IAA deficient diet, but wortmannin increased ERK activation and intake of the deficient diet before 40min and PD98059 acted after 40min to increase the second meal. Wortmannin 72-82 mitogen-activated protein kinase 1 Homo sapiens 93-96 18953090-5 2008 It was also shown that protective effects of DHEA, DHEAS and PGL against staurosporine-induced LDH release were attenuated by extracellular signal-regulated kinase (ERK)--mitogen-activated protein kinase (MAPK) inhibitor--PD 98059 (5 microM) but not by phosphatidylinositol-3-kinase (PI3-K) inhibitors such as LY 294002 (1 microM) or wortmannin (10 nM). Wortmannin 334-344 mitogen-activated protein kinase 1 Homo sapiens 126-163 18953090-5 2008 It was also shown that protective effects of DHEA, DHEAS and PGL against staurosporine-induced LDH release were attenuated by extracellular signal-regulated kinase (ERK)--mitogen-activated protein kinase (MAPK) inhibitor--PD 98059 (5 microM) but not by phosphatidylinositol-3-kinase (PI3-K) inhibitors such as LY 294002 (1 microM) or wortmannin (10 nM). Wortmannin 334-344 mitogen-activated protein kinase 1 Homo sapiens 165-168 17878755-5 2007 The migration can also be nearly completely blocked by phosphoinositide 3-kinase inhibitors (LY294002 and wortmannin) and eNOS inhibitor (N-nitro-arginine methyl ester), whereas mitogen-activated protein kinase/ERK inhibitor (PD98059) had no significant effect on SDF-1alpha-induced migration. Wortmannin 106-116 mitogen-activated protein kinase 1 Homo sapiens 211-214 17724683-4 2007 BCR-mediated phosphorylation of Akt and Erk is sensitive to the PI3K catalytic inhibitor wortmannin in both marginal zone (MZ) and follicular (FO) cells. Wortmannin 89-99 mitogen-activated protein kinase 1 Homo sapiens 40-43 18515984-6 2008 The expression of PSA, KLK10 and KLK11 was repressed by the MEK1/2 inhibitor U0126 and the PI3K inhibitor Wortmannin in the presence of the hormone, thus implicating the RAS/MEK/ERK and PI3K/AKT signaling pathways in regulating hormone-dependent KLK gene activation. Wortmannin 106-116 mitogen-activated protein kinase 1 Homo sapiens 178-181 17643958-5 2007 In addition, Cch-induced MAPK/ERK phosphorylation was partially inhibited by LY294002 and wortmannin, two selective inhibitors of phosphatidylinositol 3-kinase (PI3K), tyrphostin AG1478, a specific inhibitor of epidermal growth factor receptor (EGFR) kinase, and (-)-perillic acid, a post-translational inhibitor of small G-proteins isoprenylation. Wortmannin 90-100 mitogen-activated protein kinase 1 Homo sapiens 30-33 17222412-3 2007 PI3K inhibitors, LY294002 and wortmannin, inhibited the activation of Akt and ERK following the treatment with PS-liposomes. Wortmannin 30-40 mitogen-activated protein kinase 1 Homo sapiens 78-81 17303087-6 2007 Tanshinone IIA-induced Nrf2 activation was association with activation of ERK and PKB, which was prevented by treatment with PD098059 or wortmannin. Wortmannin 137-147 mitogen-activated protein kinase 1 Homo sapiens 74-77 17464189-2 2007 In this study we found that treatment of PMA-induced K562 cells with Go6976, a specific inhibitor of PKC, and U0126, an inhibitor of the extracellular signal-regulated kinase (ERK) reduced expression of GM3 synthase, whereas wortmannin, an inhibitor of phosphoinositide 3-kinase (PI3K) did not. Wortmannin 225-235 mitogen-activated protein kinase 1 Homo sapiens 176-179 16641198-8 2006 The aggravation of brain injury by Wortmannin was associated with the restitution of p38, but not of JNK-1/-2, ERK-1/-2, or inducible NOS (iNOS). Wortmannin 35-45 mitogen-activated protein kinase 1 Homo sapiens 85-88 17308073-6 2007 In contrast, simultaneous inactivation of the EGFR downstream targets mitogen-activated protein/extracellular signal-regulated kinase (ERK) kinase and PI3K by U0126 and wortmannin triggered rapid tumor cell death. Wortmannin 169-179 mitogen-activated protein kinase 1 Homo sapiens 135-138 16100051-7 2005 In addition, adenosine- and CCPA-induced phosphorylation of ERK, JNK, and AKT is inhibited, respectively, by U0126, PD98059 (mitogen-activated protein kinase kinase inhibitors), SP600125 (JNK kinase inhibitor), and wortmannin (phosphatidylinositol 3-kinase inhibitor). Wortmannin 215-225 mitogen-activated protein kinase 1 Homo sapiens 60-63 16458614-3 2006 TNF-alpha significantly increased extracellular signal-related kinases (ERK) and protein kinase B (PKB) activation and, which was blocked by notoginsenoside R1, PD098059, U0126 or wortmannin. Wortmannin 180-190 mitogen-activated protein kinase 1 Homo sapiens 34-70 16458614-3 2006 TNF-alpha significantly increased extracellular signal-related kinases (ERK) and protein kinase B (PKB) activation and, which was blocked by notoginsenoside R1, PD098059, U0126 or wortmannin. Wortmannin 180-190 mitogen-activated protein kinase 1 Homo sapiens 72-75 16458614-4 2006 Our data demonstrates that TNF-alpha-induced up-regulation of PAI-1 mRNA and protein levels and secretion occur via activation of ERK and PKB, which was prevented by treatment of notoginsenoside R1, PD098059, U0126 or wortmannin. Wortmannin 218-228 mitogen-activated protein kinase 1 Homo sapiens 130-133 16914897-6 2006 In addition, activation of the MAPK/ ERK cascade by ATP was blocked in cells pretreated with wortmannin and LY294002, but not by U73122, BAPTA or a Ca(2+)-free medium. Wortmannin 93-103 mitogen-activated protein kinase 1 Homo sapiens 37-40 15744749-5 2005 Pretreatment of human breast cancer cells with wortmannin or LY294002, selective inhibitors of phosphoinositide 3-kinase (PI3K), diminished Cch-mediated MAPK/ERK phosphorylation. Wortmannin 47-57 mitogen-activated protein kinase 1 Homo sapiens 158-161 15492467-3 2004 Twelve hours after treatment with oridonin, the ratio of Bax/Bcl-xL protein expression was increased and release of cytochrome c was decreased by an extracellular signal-regulated kinase (ERK) MAPK inhibitor (PD98059) and a phosphoinositide 3-kinases (PI3-K) inhibitor (wortmannin). Wortmannin 270-280 mitogen-activated protein kinase 1 Homo sapiens 188-191 15572039-9 2005 Finally, the proliferative, antiapoptotic, and cytoskeletal effects of constitutively active Ras in MG63 cells on the hydrophobic surface were blocked by wortmannin and PD98059 that inhibit Akt and ERK activation, respectively. Wortmannin 154-164 mitogen-activated protein kinase 1 Homo sapiens 198-201 14705145-9 2004 In fact, in the presence of the specific PKC inhibitor GF 109203X, or the specific PI 3-K inhibitor wortmannin, we observed a significant dose-dependent reduction in ERK phosphorylation. Wortmannin 100-110 mitogen-activated protein kinase 1 Homo sapiens 166-169 15177934-8 2004 The effects of NT on EGFR/ERK/Akt activation and DNA synthesis were attenuated by PLC-inhibitor (U73122), PKC-inhibitors (bisindolylmaleimide, staurosporine, rottlerin), MEK inhibitor (U0126) and PI3 kinase inhibitors (wortmannin, LY 294002). Wortmannin 219-229 mitogen-activated protein kinase 1 Homo sapiens 26-29 15194438-8 2004 Moreover, the specific PI3K inhibitor, Wortmannin, abolished MAPK/ERK and Elk-1 phosphorylation in HGF-treated cells, suggesting the requirement of PI3K in mediating the HGF-induced MAPK pathway. Wortmannin 39-49 mitogen-activated protein kinase 1 Homo sapiens 66-69 15110780-3 2004 Using sublingual gland acinar cells, we show that prosecretory effect of isoproterenol on phospholipid release was subjected to suppression by EGFR kinase inhibitor, PD153035, and wortmannin, an inhibitor of PI3K, but not by PD98059, an inhibitor of extracellular signal regulated kinase (ERK). Wortmannin 180-190 mitogen-activated protein kinase 1 Homo sapiens 250-287 15110780-3 2004 Using sublingual gland acinar cells, we show that prosecretory effect of isoproterenol on phospholipid release was subjected to suppression by EGFR kinase inhibitor, PD153035, and wortmannin, an inhibitor of PI3K, but not by PD98059, an inhibitor of extracellular signal regulated kinase (ERK). Wortmannin 180-190 mitogen-activated protein kinase 1 Homo sapiens 289-292 14660637-8 2004 Active peptides also activated protein kinase B and extracellular signal-regulated kinase (ERK) in vivo in a wortmannin-sensitive manner while reducing recoverable cellular p85 levels. Wortmannin 109-119 mitogen-activated protein kinase 1 Homo sapiens 52-89 14660637-8 2004 Active peptides also activated protein kinase B and extracellular signal-regulated kinase (ERK) in vivo in a wortmannin-sensitive manner while reducing recoverable cellular p85 levels. Wortmannin 109-119 mitogen-activated protein kinase 1 Homo sapiens 91-94 14563359-6 2003 Experiments based on inhibitors of specific signaling pathways, such as manumycin A, toxin A, U0126, PD98059 and wortmannin revealed that Ras, MEK and PI-3K are involved in the activation of ERK. Wortmannin 113-123 mitogen-activated protein kinase 1 Homo sapiens 191-194 12942541-7 2003 The previously reported proliferation of MCF-7 cells induced by Ang II was blocked by PD098059 and by wortmannin in a dose-dependent manner, thereby indicating that in MCF-7 cells the PI3K and ERK pathways mediate the mitogenic signalling of AT1. Wortmannin 102-112 mitogen-activated protein kinase 1 Homo sapiens 193-196 14516797-8 2003 Inhibition of phosphatidylinositol-3"-kinase (PI3K) by wortmannin inhibited adenosine A(2B) receptor-mediated ERK1/2 phosphorylation and activation of Rap1, without affecting CREB and p38 phosphorylation. Wortmannin 55-65 mitogen-activated protein kinase 1 Homo sapiens 184-187 12444151-3 2002 We found that, in addition to the MAP/Erk kinase inhibitor PD098059, the PI 3-kinase inhibitors LY294002 and wortmannin both suppressed Erk activation in M-CSF-treated monocytes, suggesting that 3-phosphorylated products of PI 3-kinase played a role in Erk activation. Wortmannin 109-119 mitogen-activated protein kinase 1 Homo sapiens 136-139 12943808-13 2003 Activation of p38(MAPK) was significantly inhibited by SB203580, pertussis toxin, Wortmannin, and LY294002, but not by PD98059 or AG1478; MAPKAP kinase-2 activation was inhibited by SB203580. Wortmannin 82-92 mitogen-activated protein kinase 1 Homo sapiens 14-17 12681450-6 2003 This report shows that the PI3K inhibitors LY294002 and wortmannin block activation of MEK and ERK by IL-2 in primary human T cells. Wortmannin 56-66 mitogen-activated protein kinase 1 Homo sapiens 95-98 12444151-3 2002 We found that, in addition to the MAP/Erk kinase inhibitor PD098059, the PI 3-kinase inhibitors LY294002 and wortmannin both suppressed Erk activation in M-CSF-treated monocytes, suggesting that 3-phosphorylated products of PI 3-kinase played a role in Erk activation. Wortmannin 109-119 mitogen-activated protein kinase 1 Homo sapiens 136-139 12444151-4 2002 Investigating the biochemical pathways regulated by PI 3-kinase to activate Erk, we found that, in response to M-CSF, normal human monocytes induced reactive oxygen species (ROS), which were suppressed by the PI 3-kinase inhibitor wortmannin but not by the solvent control DMSO or the MAP/Erk kinase inhibitor PD098059. Wortmannin 231-241 mitogen-activated protein kinase 1 Homo sapiens 76-79 12444151-4 2002 Investigating the biochemical pathways regulated by PI 3-kinase to activate Erk, we found that, in response to M-CSF, normal human monocytes induced reactive oxygen species (ROS), which were suppressed by the PI 3-kinase inhibitor wortmannin but not by the solvent control DMSO or the MAP/Erk kinase inhibitor PD098059. Wortmannin 231-241 mitogen-activated protein kinase 1 Homo sapiens 289-292 12296652-9 2002 Wortmannin (500 and 1000 nM) significantly decreased PDGF-BB stimulation of meningioma cells (p < 0.001) while it reduced Akt and p70S6K phosphorylation but not mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) phosphorylation. Wortmannin 0-10 mitogen-activated protein kinase 1 Homo sapiens 236-240 12296652-9 2002 Wortmannin (500 and 1000 nM) significantly decreased PDGF-BB stimulation of meningioma cells (p < 0.001) while it reduced Akt and p70S6K phosphorylation but not mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) phosphorylation. Wortmannin 0-10 mitogen-activated protein kinase 1 Homo sapiens 241-244 11445586-6 2001 Western blot analyses using antibodies specific for phosphorylated p38 showed an enhanced activation of this MAP kinase in human umbilical cord vein endothelial cells when stimulated with VEGF in the presence of wortmannin in comparison to either agent alone. Wortmannin 212-222 mitogen-activated protein kinase 1 Homo sapiens 67-70 12055097-2 2002 We found that phosphatidylinositol 3-kinase (PI3K) is activated in OSE cells in response to elevated extracellular calcium, and the PI3K inhibitors wortmannin and LY-294002 inhibited extracellular signal-regulated kinase (ERK) activation by approximately 75%, similar to effects of the mitogen-activated protein kinase/ERK kinase inhibitor PD-98059. Wortmannin 148-158 mitogen-activated protein kinase 1 Homo sapiens 183-220 12055097-2 2002 We found that phosphatidylinositol 3-kinase (PI3K) is activated in OSE cells in response to elevated extracellular calcium, and the PI3K inhibitors wortmannin and LY-294002 inhibited extracellular signal-regulated kinase (ERK) activation by approximately 75%, similar to effects of the mitogen-activated protein kinase/ERK kinase inhibitor PD-98059. Wortmannin 148-158 mitogen-activated protein kinase 1 Homo sapiens 222-225 12055097-2 2002 We found that phosphatidylinositol 3-kinase (PI3K) is activated in OSE cells in response to elevated extracellular calcium, and the PI3K inhibitors wortmannin and LY-294002 inhibited extracellular signal-regulated kinase (ERK) activation by approximately 75%, similar to effects of the mitogen-activated protein kinase/ERK kinase inhibitor PD-98059. Wortmannin 148-158 mitogen-activated protein kinase 1 Homo sapiens 319-322 11994364-6 2002 PI3K blockade by wortmannin, known to prevent G protein betagamma subunit effect on ERK1 and -2, induced a 30% reduction of the Ca(o)(2+)-stimulated ERK1 and -2 activity. Wortmannin 17-27 mitogen-activated protein kinase 1 Homo sapiens 84-95 11687581-7 2001 Furthermore, pretreatment with wortmannin, an inhibitor of phosphatidylinositol 3 (PI3)-kinase, inhibited 15-d-PGJ2-induced ERK activation and c-fos mRNA expression, suggesting that PI3-kinase is involved in the process. Wortmannin 31-41 mitogen-activated protein kinase 1 Homo sapiens 124-127 10545192-5 1999 Preincubation with wortmannin and LY294002, two structurally and mechanistically different inhibitors of PI3-K, blocked the VS-mediated increase in MAPK activity and phosphorylation of ERK-1 and ERK-2. Wortmannin 19-29 mitogen-activated protein kinase 1 Homo sapiens 195-200 11590203-4 2001 Activation of both ERK and NF-kappaB was blocked by wortmannin and LY294002, specific inhibitors of PI 3-K. Wortmannin also inhibited the Fc receptor-mediated increase in the cytosolic calcium concentration, but it did not block immunoglobulin G (IgG)-mediated phagocytosis. Wortmannin 52-62 mitogen-activated protein kinase 1 Homo sapiens 19-22 11590203-4 2001 Activation of both ERK and NF-kappaB was blocked by wortmannin and LY294002, specific inhibitors of PI 3-K. Wortmannin also inhibited the Fc receptor-mediated increase in the cytosolic calcium concentration, but it did not block immunoglobulin G (IgG)-mediated phagocytosis. Wortmannin 108-118 mitogen-activated protein kinase 1 Homo sapiens 19-22 11241670-6 2001 Wortmannin (500 nM), a specific inhibitor of phosphatidylinositol 3-kinase ( PI 3-K), inhibited the activation of ERK1 and ERK2 by PDGF-BB but not by FGF-2 suggesting that PI 3-K mediated the activation of ERK MAPK pathway by PDGF-BB but not by FGF-2. Wortmannin 0-10 mitogen-activated protein kinase 1 Homo sapiens 123-127 10996851-5 2000 On the other hand, rottlerin (the selective PKCdelta inhibitor), SB 203580 (the selective p38 MAPK inhibitor), and wortmannin (the PI 3-kinase inhibitor) can affect neither cPLA(2) nor ERK phosphorylation. Wortmannin 115-125 mitogen-activated protein kinase 1 Homo sapiens 185-188 11016663-8 2000 Inhibition by wortmannin of PI3-kinase activity, which has been implicated in ET-1 and other G protein-coupled receptor (GPCR)-mediated signaling pathways, reduced Erk 2 activation by ET-1 but had no effect on ET-1-induced EGF-R and Shc phosphorylation. Wortmannin 14-24 mitogen-activated protein kinase 1 Homo sapiens 164-169 10898493-5 2000 The Ca2+ chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N",N"-tetraacetic acid, BIM-I and wortmannin each had only a small effect on ERK2 activation induced by surface IgM alone but blocked the enhancement of that activation by CD1 9/mIgM coligation. Wortmannin 86-96 mitogen-activated protein kinase 1 Homo sapiens 129-133 10792610-11 2000 Using two structurally unrelated PI3K inhibitors, wortmanin and LY294002, both tyrosine phosphorylation of Cx43 and activation of ERK stimulated by PDGF were largely blocked. Wortmannin 50-59 mitogen-activated protein kinase 1 Homo sapiens 130-133 10565839-4 1999 ERK activation by 100 nM NPY was abolished by the Y(1) NPY receptor antagonist BIBP 3226 (1 microM), pertussis toxin treatment (100 ng ml(-1) overnight), the mitogen-activated protein kinase (MAPK) kinase inhibitor PD 98059 (100 microM), and the phosphatidylinositol-3-kinase inhibitor wortmannin (100 nM). Wortmannin 286-296 mitogen-activated protein kinase 1 Homo sapiens 0-3 10508274-4 1999 Wortmannin, an inhibitor of phosphatidylinositol 3-kinase, partially inhibited ERK2 and p38 activation triggered through the B cell receptor whereas activation of JNK1 and p38 through CD40 was not affected. Wortmannin 0-10 mitogen-activated protein kinase 1 Homo sapiens 79-83 10508274-4 1999 Wortmannin, an inhibitor of phosphatidylinositol 3-kinase, partially inhibited ERK2 and p38 activation triggered through the B cell receptor whereas activation of JNK1 and p38 through CD40 was not affected. Wortmannin 0-10 mitogen-activated protein kinase 1 Homo sapiens 88-91 9927616-8 1999 Furthermore, the activation of ERK by the beta-3 AR is sensitive to PD98059, wortmannin, and LY294002, indicating a crucial role for mitogen-activated protein kinase kinase and phosphatidylinositol-3 kinase (PI3K), respectively. Wortmannin 77-87 mitogen-activated protein kinase 1 Homo sapiens 31-34