PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 18941890-11 2009 Inhibition of Erk1/2 by U0126 (10 microM) decreased p-ser118-ERalpha by 51.7 +/- 8.5% and decreased p-ser167-ERalpha by 41.9 +/- 16.9% whereas inhibition of Akt by LY294002 (20 microM) and wortmannin (500 nM) or by siRNA knock-down, had no effect on p-ser167-ERalpha expression. Wortmannin 189-199 mitogen-activated protein kinase 3 Homo sapiens 14-20 17188372-4 2007 NDP-MSH-induced ERK1/2 phosphorylation was sensitive to pertussis toxin and the PI3K inhibitor, wortmannin. Wortmannin 96-106 mitogen-activated protein kinase 3 Homo sapiens 16-22 19344769-7 2009 The use of Wortmannin or PD98059 demonstrated the involvement of the PI3K/Akt as well as the ERK1/2 transduction pathways in the H(C) fragment effect. Wortmannin 11-21 mitogen-activated protein kinase 3 Homo sapiens 93-99 19428994-8 2009 However, alpha-MSH-induced ERK1/2 activation is abolished by the phosphatidylinositol 3-kinase (PI3K) inhibitors wortmannin and LY294002. Wortmannin 113-123 mitogen-activated protein kinase 3 Homo sapiens 27-33 17186496-3 2007 A sequential analysis of the obestatin transmembrane signaling pathway showed that the ERK 1/2 activity is partially blocked after preincubation of the cells with pertussis toxin (PTX), as well as by wortmannin (an inhibitor of PI3K), claphostin C (an inhibitor of PKC), and PP2 (which inhibits the non receptor tyrosine kinase Src). Wortmannin 200-210 mitogen-activated protein kinase 3 Homo sapiens 87-94 17275785-9 2007 Inhibition of insulin- and A-II-stimulated phosphorylation of ERK5 and ERK1/2 by PD98059 and Wortmannin exhibited differential and time-dependent effects. Wortmannin 93-103 mitogen-activated protein kinase 3 Homo sapiens 71-77 19671683-8 2009 Blocking the activations of ERK1/2 and AKT by MKK1/2 inhibitor (U0126) and phosphoinositide 3-kinase inhibitor (wortmannin) suppressed the expression of Rad51 and enhanced the erlotinib-induced cell death in erlotinib-resistant cells. Wortmannin 112-122 mitogen-activated protein kinase 3 Homo sapiens 28-34 17975262-6 2007 Furthermore, SB203580 and PD098059 act as inhibitors of p38MAPK and ERK1/2 respectively, wortmannin, an inhibitor of phosphatidylinositol-3-kinase, and cyclosporin A, a calcineurin inhibitor, hindered both translocation of Rac2 from the cytosol to the plasma membrane and enhancement of Rac2 GTP-binding elicited by Ang II. Wortmannin 89-99 mitogen-activated protein kinase 3 Homo sapiens 68-74 17827719-5 2007 Inhibition of PI3K activity by wortmannin completely inhibited KRGE-induced angiogenesis and phosphorylation of Akt, ERK1/2, and eNOS, indicating that PI3K/Akt activation is an upstream event of the KRGE-mediated angiogenic pathway. Wortmannin 31-41 mitogen-activated protein kinase 3 Homo sapiens 117-123 17539658-8 2007 EGCG also decreased the phosphorylation of Akt and extracellular signal-regulated kinase 1/2 that were demonstrated as selected downstream HRG-beta1-responsive kinases required for FAS expression using dominant-negative Akt, PI3K inhibitors (LY294002 and wortmannin), or MEK inhibitor (PD98059). Wortmannin 255-265 mitogen-activated protein kinase 3 Homo sapiens 51-92 17350964-6 2007 Experiments using wortmannin and PD98059, specific inhibitors of the PI3K/Akt and ERK1/2 activity, respectively, showed that the activation of both pathways is required for growth-factor-induced cell motility responses. Wortmannin 18-28 mitogen-activated protein kinase 3 Homo sapiens 82-88 16207820-7 2005 Intracerebroventricular injections of selective inhibitors of ERK-1/-2 (PD98059) or Akt (wortmannin) pathways revealed that both ERK-1/-2 and Akt were required for Epo"s neuroprotective function, antagonization of either pathway completely abolishing tissue protection. Wortmannin 89-99 mitogen-activated protein kinase 3 Homo sapiens 129-137 16630729-4 2006 PD98059 and wortmannin, which are, respectively, specific inhibitors of the extracellular regulated kinase1/2 (ERK1/2) and phosphatidylinositol-3 kinase (PI-3K) signal pathways, markedly attenuate this stimulation of proliferation. Wortmannin 12-22 mitogen-activated protein kinase 3 Homo sapiens 111-117 16406609-3 2006 Here, we report that two structurally distinct PI3K inhibitors, wortmannin and LY294002, inhibited insulin-induced activation of ERK1/2 but had no effect on EGF-induced activation of ERK1/2 in hepatocellular carcinoma BEL-7402 and SMMC-7721 cells, breast cancer MCF-7 cells, and prostate cancer LNCaP cells. Wortmannin 64-74 mitogen-activated protein kinase 3 Homo sapiens 129-135 16239258-8 2006 Furthermore, in the presence of the inhibitors of MAPK and phosphatidylinositol 3-kinase pathways, PD 98059 and wortmannin, respectively, E2 and OHT did not elicit growth stimulation. Wortmannin 112-122 mitogen-activated protein kinase 3 Homo sapiens 50-54 16166591-4 2006 This induction is attributable to transcriptional up-regulation of TF gene expression dependent upon both activation of the p65/c-Rel TF-kappaB transcription factor and activity of the ERK1/2 kinase pathway upstream of Egr-1 and was markedly further enhanced in the presence of wortmannin, an inhibitor of the PI3 kinase/Akt pathway. Wortmannin 278-288 mitogen-activated protein kinase 3 Homo sapiens 185-191 15468165-10 2005 mitogen-activated protein kinase kinase (MEK) and phosphoinositide 3-kinase (PI3K) appear to be also involved since the inhibitors PD98059 and wortmannin abolished ERK1/2 phosphorylation and decreased NE differentiation induced by VIP. Wortmannin 143-153 mitogen-activated protein kinase 3 Homo sapiens 164-170 16128807-3 2005 In three different hemopoietic cell lines stimulated with cytokines, and in HEK293 cells, stimulated with serum, either wortmannin or LY294002, but never both, could partially block phosphorylation of Erks. Wortmannin 120-130 mitogen-activated protein kinase 3 Homo sapiens 201-205 14751283-7 2004 TGF-beta1-mediated NF-kappabeta activation was blocked by wortmannin and U0126, indicating the involvement of both phosphatidylinositol-3-OH kinase (PI3k)/Akt and mitogen-activated protein kinase (MAPK)/extracellular-signal regulated kinase (Erk)1,2 pathways in the action of TGF-beta1. Wortmannin 58-68 mitogen-activated protein kinase 3 Homo sapiens 203-249 15313431-4 2004 We found that C5a-induced, time-dependent (1) ERK1/2 phosphorylation was markedly diminished by PTX, U73122, P13K inhibitors wortmannin and LY294002 and ERK1/2 inhibitor PD98059; (2) Akt phosphorylation was also attenuated by the above inhibitors except PD98059; (3) p38 MAPK phosphorylation was only affected by PTX. Wortmannin 125-135 mitogen-activated protein kinase 3 Homo sapiens 46-52 15235105-7 2004 However, it induced cell migration in the presence of EGF and Wortmannin, in which both AKT and MAPK pathways were activated. Wortmannin 62-72 mitogen-activated protein kinase 3 Homo sapiens 96-100 15069082-9 2004 Notably, insulin-stimulated ERK1/2 phosphorylation was abolished by wortmannin and GF109203X in HSMCs. Wortmannin 68-78 mitogen-activated protein kinase 3 Homo sapiens 28-34 14985355-7 2004 Wortmannin and LY294002, inhibitors of phosphoinositide 3-kinase, and PD98059, an inhibitor of MEK, abrogated survival and capillary tube formation, indicating that Akt and Erk1/2 should promote survival and capillary tube formation of these endothelial cells at a locus downstream to stem cell factor/c-kit signaling. Wortmannin 0-10 mitogen-activated protein kinase 3 Homo sapiens 173-179 14751283-9 2004 Interestingly, the increased phosphorylation of IKKalpha/beta and Ikappabetaalpha was abrogated by wortmannin, but not by U0126, suggesting that PI3k/Akt and MAPK/Erk1,2 pathways triggered by TGF-beta1 regulated the activation of NF-kappabeta through different mechanisms. Wortmannin 99-109 mitogen-activated protein kinase 3 Homo sapiens 163-169 14516795-6 2003 While the phosphatidylinositol 3-kinase (PI3K) inhibitors wortmannin and LY294002 completely abrogated arsenite activation of p70(s6k), ERK1/2 activation by arsenite was not affected by these inhibitors, indicating that H(2)O(2) might act as an upstream molecule of PI3K as well as ERK1/2. Wortmannin 58-68 mitogen-activated protein kinase 3 Homo sapiens 136-142 14516797-8 2003 Inhibition of phosphatidylinositol-3"-kinase (PI3K) by wortmannin inhibited adenosine A(2B) receptor-mediated ERK1/2 phosphorylation and activation of Rap1, without affecting CREB and p38 phosphorylation. Wortmannin 55-65 mitogen-activated protein kinase 3 Homo sapiens 110-116 14516795-6 2003 While the phosphatidylinositol 3-kinase (PI3K) inhibitors wortmannin and LY294002 completely abrogated arsenite activation of p70(s6k), ERK1/2 activation by arsenite was not affected by these inhibitors, indicating that H(2)O(2) might act as an upstream molecule of PI3K as well as ERK1/2. Wortmannin 58-68 mitogen-activated protein kinase 3 Homo sapiens 282-288 12505872-5 2003 The increase in cell numbers was blocked by pretreatment with RP8-CPT-cGMP (RP8), a cGMP-dependent protein kinase inhibitor, with wortmannin, an Akt/PKB inhibitor, and with PD-98059, an ERK1/2 inhibitor. Wortmannin 130-140 mitogen-activated protein kinase 3 Homo sapiens 186-192 12689930-6 2003 PlGF-mediated cytochemokine mRNA and protein expression was inhibited by PD98059 and wortmannin, inhibitors of mitogen-activated protein kinase kinase (MAPK/MEK) kinase and phosphatidylinositol-3 (PI3) kinase, respectively, but not by SB203580, a p38 kinase inhibitor. Wortmannin 85-95 mitogen-activated protein kinase 3 Homo sapiens 152-156 12689930-7 2003 PlGF caused a time-dependent transient increase in phosphorylation of extracellular signal-regulated kinase-1/2 (ERK-1/2), which was completely inhibited by wortmannin, indicating that activation of PI3 kinase preceded MEK activation. Wortmannin 157-167 mitogen-activated protein kinase 3 Homo sapiens 70-111 12689930-7 2003 PlGF caused a time-dependent transient increase in phosphorylation of extracellular signal-regulated kinase-1/2 (ERK-1/2), which was completely inhibited by wortmannin, indicating that activation of PI3 kinase preceded MEK activation. Wortmannin 157-167 mitogen-activated protein kinase 3 Homo sapiens 113-120 12411472-6 2002 Because activation of two signaling pathways, the phosphatidylinositol-3 kinase (PI3K)-->Akt-->eNOS and the ERK1/2 MAPK pathway, is known to be involved in cell migration, we used the pharmacological inhibitors wortmannin and PD98059 to determine if chemotactic signaling by leptin involves Akt or ERK1/2, respectively. Wortmannin 217-227 mitogen-activated protein kinase 3 Homo sapiens 114-120 12414747-10 2002 LAM-induced phosphorylation of p42 (extracellular signal-regulated kinase 2) was further enhanced by wortmannin, a PI3 kinase inhibitor. Wortmannin 101-111 mitogen-activated protein kinase 3 Homo sapiens 36-75 12411472-6 2002 Because activation of two signaling pathways, the phosphatidylinositol-3 kinase (PI3K)-->Akt-->eNOS and the ERK1/2 MAPK pathway, is known to be involved in cell migration, we used the pharmacological inhibitors wortmannin and PD98059 to determine if chemotactic signaling by leptin involves Akt or ERK1/2, respectively. Wortmannin 217-227 mitogen-activated protein kinase 3 Homo sapiens 121-125 12411472-6 2002 Because activation of two signaling pathways, the phosphatidylinositol-3 kinase (PI3K)-->Akt-->eNOS and the ERK1/2 MAPK pathway, is known to be involved in cell migration, we used the pharmacological inhibitors wortmannin and PD98059 to determine if chemotactic signaling by leptin involves Akt or ERK1/2, respectively. Wortmannin 217-227 mitogen-activated protein kinase 3 Homo sapiens 304-310 11994364-6 2002 PI3K blockade by wortmannin, known to prevent G protein betagamma subunit effect on ERK1 and -2, induced a 30% reduction of the Ca(o)(2+)-stimulated ERK1 and -2 activity. Wortmannin 17-27 mitogen-activated protein kinase 3 Homo sapiens 149-160 12191495-3 2002 In addition muscarine-enhanced sAPPalpha secretion and ERK1/2 activation were inhibited 60 and 80%, respectively, by micromolar concentrations of the phosphatidylinositol 3 kinase (PI-3K) inhibitor wortmannin. Wortmannin 198-208 mitogen-activated protein kinase 3 Homo sapiens 55-61 12191495-4 2002 In comparison wortmannin decreased EGF stimulation of sAPPalpha secretion and ERK 1/2 activation by approximately 40%. Wortmannin 14-24 mitogen-activated protein kinase 3 Homo sapiens 78-85 11241670-6 2001 Wortmannin (500 nM), a specific inhibitor of phosphatidylinositol 3-kinase ( PI 3-K), inhibited the activation of ERK1 and ERK2 by PDGF-BB but not by FGF-2 suggesting that PI 3-K mediated the activation of ERK MAPK pathway by PDGF-BB but not by FGF-2. Wortmannin 0-10 mitogen-activated protein kinase 3 Homo sapiens 114-118 11902114-4 2002 We now report that NMDA receptor activation of Erk1/2 was also blocked by inhibitors of PI 3-kinase (LY 294002, wortmannin). Wortmannin 112-122 mitogen-activated protein kinase 3 Homo sapiens 47-53 11739687-6 2002 The E5-mediated protection against apoptosis was blocked by wortmannin and PD98059, specific inhibitors of the PI3K and ERK1/2 MAPK pathways, respectively, suggesting that the PI3K and ERK1/2 MAPK pathways are involved in this process. Wortmannin 60-70 mitogen-activated protein kinase 3 Homo sapiens 120-126 11739687-6 2002 The E5-mediated protection against apoptosis was blocked by wortmannin and PD98059, specific inhibitors of the PI3K and ERK1/2 MAPK pathways, respectively, suggesting that the PI3K and ERK1/2 MAPK pathways are involved in this process. Wortmannin 60-70 mitogen-activated protein kinase 3 Homo sapiens 127-131 11739687-6 2002 The E5-mediated protection against apoptosis was blocked by wortmannin and PD98059, specific inhibitors of the PI3K and ERK1/2 MAPK pathways, respectively, suggesting that the PI3K and ERK1/2 MAPK pathways are involved in this process. Wortmannin 60-70 mitogen-activated protein kinase 3 Homo sapiens 185-191 11739687-6 2002 The E5-mediated protection against apoptosis was blocked by wortmannin and PD98059, specific inhibitors of the PI3K and ERK1/2 MAPK pathways, respectively, suggesting that the PI3K and ERK1/2 MAPK pathways are involved in this process. Wortmannin 60-70 mitogen-activated protein kinase 3 Homo sapiens 192-196 11532940-5 2001 At least some of these signals are transduced via a protein kinase C-dependent activation of the p42/p44 MAPK signalling cascade and via a wortmannin-sensitive induction of Akt phosphorylation. Wortmannin 139-149 mitogen-activated protein kinase 3 Homo sapiens 101-109 11267999-4 2001 Accordingly, ERK1/2 phosphorylation induced by UTP was inhibited by the PI3K inhibitors, wortmannin and LY294002, and the c-src inhibitors, radicicol and PP2, but not by inhibitors of protein kinase C (PKC). Wortmannin 89-99 mitogen-activated protein kinase 3 Homo sapiens 13-19 11241670-6 2001 Wortmannin (500 nM), a specific inhibitor of phosphatidylinositol 3-kinase ( PI 3-K), inhibited the activation of ERK1 and ERK2 by PDGF-BB but not by FGF-2 suggesting that PI 3-K mediated the activation of ERK MAPK pathway by PDGF-BB but not by FGF-2. Wortmannin 0-10 mitogen-activated protein kinase 3 Homo sapiens 210-214 10873605-3 2000 Both Akt and ERK1/2 were rapidly phosphorylated by IGF-1 and blocked by wortmannin and PD98059, inhibitors of their upstream activators respectively. Wortmannin 72-82 mitogen-activated protein kinase 3 Homo sapiens 13-19 10545192-5 1999 Preincubation with wortmannin and LY294002, two structurally and mechanistically different inhibitors of PI3-K, blocked the VS-mediated increase in MAPK activity and phosphorylation of ERK-1 and ERK-2. Wortmannin 19-29 mitogen-activated protein kinase 3 Homo sapiens 148-152 10545192-5 1999 Preincubation with wortmannin and LY294002, two structurally and mechanistically different inhibitors of PI3-K, blocked the VS-mediated increase in MAPK activity and phosphorylation of ERK-1 and ERK-2. Wortmannin 19-29 mitogen-activated protein kinase 3 Homo sapiens 185-190 10380914-5 1999 We observed that wortmannin, LY 294002, and PD 098059 reduce basophil ERK-1,2 activation, thus implying that, with regard to arachidonic acid metabolism, MEK kinases are a downstream target for PI-3-kinase. Wortmannin 17-27 mitogen-activated protein kinase 3 Homo sapiens 70-77 10506137-7 1999 PD98059, an inhibitor of ERK1/2 activation, completely suppressed the adaptive increase of system A transport activity that, conversely, was unaffected by inhibitors of other transduction pathways, such as rapamycin and wortmannin, as well as by chronic treatment with phorbol esters. Wortmannin 220-230 mitogen-activated protein kinase 3 Homo sapiens 25-31 10698349-6 1999 ERK1 activity was inhibited by wortmannin suggesting that ERK1 pathway is activated through phosphatidyl inositol -3 (PI 3-) kinase. Wortmannin 31-41 mitogen-activated protein kinase 3 Homo sapiens 0-4 10698349-6 1999 ERK1 activity was inhibited by wortmannin suggesting that ERK1 pathway is activated through phosphatidyl inositol -3 (PI 3-) kinase. Wortmannin 31-41 mitogen-activated protein kinase 3 Homo sapiens 58-62 10051434-5 1999 We have also shown that phosphoinositide 3-kinase (PI-3K) inhibitors (wortmannin and LY294002) decreased p42/p44 MAPK activation. Wortmannin 70-80 mitogen-activated protein kinase 3 Homo sapiens 113-117 10208841-5 1999 Wortmannin inhibited strain-induced ERK1/2 activation by 56.3 and 86.3 %, respectively. Wortmannin 0-10 mitogen-activated protein kinase 3 Homo sapiens 36-42 10066822-6 1999 Treatment with the PI 3-kinase inhibitors wortmannin and LY294002 also blocked MP-induced p42/p44 MAPK, p38, and JNK1 phosphorylation. Wortmannin 42-52 mitogen-activated protein kinase 3 Homo sapiens 94-102 9531494-7 1998 CPPD crystals also activated the ERK1 and ERK2 forms of MAP kinase (wortmannin insensitive), PKC (Compound 3 sensitive) and protein kinase B (wortmannin sensitive) in neutrophils. Wortmannin 68-78 mitogen-activated protein kinase 3 Homo sapiens 33-37 9882612-8 1999 This is confirmed here by experiments which showed that PI3K inhibitors (wortmannin and LY294002) reduced the activation of p42/p44 MAPK by PDGF. Wortmannin 73-83 mitogen-activated protein kinase 3 Homo sapiens 132-136 10576564-13 1999 However, pretreatment with wortmannin or LY294002, the inhibitor of phosphatidylinositol 3 kinase (PI-3K), strongly inhibited peptide-stimulated ERK1 activation. Wortmannin 27-37 mitogen-activated protein kinase 3 Homo sapiens 145-149 9920505-6 1999 Pretreatment with wortmannin inhibits ERK1 activation by C5b-9, suggesting the involvement of phosphatidylinositol 3-kinase (PI 3-kinase). Wortmannin 18-28 mitogen-activated protein kinase 3 Homo sapiens 38-42 33582643-8 2021 However, the phosphorylation of Erk1/2 and Akt decreased when the cells were treated with GPER inhibitor G15 and phosphatidylinositide 3-kinase (PI3K) inhibitor wortmannin (WM) prior to TCBPA exposure. Wortmannin 161-171 mitogen-activated protein kinase 3 Homo sapiens 32-38 28694915-5 2017 This was validated with the use of PD98059 and Wortmannin, specific inhibitors of Erk1/2 and PI3K, respectively. Wortmannin 47-57 mitogen-activated protein kinase 3 Homo sapiens 82-88 32733640-7 2020 UO126 and wortmannin, inhibitors of ERK1/2 and PI3K/Akt signalling pathways, respectively, prevented neuroprotective effects of quercetin. Wortmannin 10-20 mitogen-activated protein kinase 3 Homo sapiens 36-42 28395342-6 2017 In addition, FGF2 increases phosphorylation of AKT, P70S6K, S6, ERK1/2, JNK, P38, and P90RSK in a time-dependent manner, and increases in their expression was suppressed by Wortmannin (a phosphatidylinositol 3-kinase [PI3K] inhibitor), U0126 (an ERK1/2 inhibitor), SP600125 (a JNK inhibitor), and SB203580 (a P38 inhibitor) based on western blot analyses. Wortmannin 173-183 mitogen-activated protein kinase 3 Homo sapiens 64-70 28395342-6 2017 In addition, FGF2 increases phosphorylation of AKT, P70S6K, S6, ERK1/2, JNK, P38, and P90RSK in a time-dependent manner, and increases in their expression was suppressed by Wortmannin (a phosphatidylinositol 3-kinase [PI3K] inhibitor), U0126 (an ERK1/2 inhibitor), SP600125 (a JNK inhibitor), and SB203580 (a P38 inhibitor) based on western blot analyses. Wortmannin 173-183 mitogen-activated protein kinase 3 Homo sapiens 246-252 30838710-11 2019 In addition, insulin markedly enhanced the phosphorylation of PI3K/AKT, p38, JNK and ERK1/2 MAPK pathways, with wortmannin or LY294002 (a PI3K-specific inhibitor) and PD98059 (a MEK1-specific inhibitor) significantly inhibiting the insulin-induced increase in MMP-2 gelatinolytic activity. Wortmannin 112-122 mitogen-activated protein kinase 3 Homo sapiens 85-91 30838710-11 2019 In addition, insulin markedly enhanced the phosphorylation of PI3K/AKT, p38, JNK and ERK1/2 MAPK pathways, with wortmannin or LY294002 (a PI3K-specific inhibitor) and PD98059 (a MEK1-specific inhibitor) significantly inhibiting the insulin-induced increase in MMP-2 gelatinolytic activity. Wortmannin 112-122 mitogen-activated protein kinase 3 Homo sapiens 92-96 31005035-12 2019 Both PD98059 and wortmannin could down-regulate the apelin mRNA expression suggesting the important signaling role of ERK1/2 MAPK and PI3K in the gene expression of apelin. Wortmannin 17-27 mitogen-activated protein kinase 3 Homo sapiens 118-124 31005035-12 2019 Both PD98059 and wortmannin could down-regulate the apelin mRNA expression suggesting the important signaling role of ERK1/2 MAPK and PI3K in the gene expression of apelin. Wortmannin 17-27 mitogen-activated protein kinase 3 Homo sapiens 125-129 28381122-11 2017 This effect of argon was abolished in the presence of wortmannin and PD98059 which inhibit prosurvival phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) and MEK/extracellular receptor kinase 1/2 (ERK 1/2), respectively, or in the presence of the mitochondrial permeability transition pore opener atractyloside, suggesting the involvement of the reperfusion injury salvage kinase pathway. Wortmannin 54-64 mitogen-activated protein kinase 3 Homo sapiens 206-213 27591897-7 2016 Inhibition of ERK1/2 phosphorylation by UO126 entirely abrogated the response of p-Ser166-Mdm2 to VEGF-A treatment, while Akt phosphorylation inhibition by wortmannin led to further elevations in p-Ser166-Mdm2. Wortmannin 156-166 mitogen-activated protein kinase 3 Homo sapiens 14-20 25817573-8 2015 Finally, we found that a similar pathway, sensitive to wortmannin-AG1024 but insensitive to marimastat, is implicated in activation of ERK signaling in HEK293 cells by endogenously expressed GPCRs coupled to Gq-protein (muscarinic M3 receptors) or coupled to Gs-protein (endothelin ETB receptors). Wortmannin 55-65 mitogen-activated protein kinase 3 Homo sapiens 135-138 27208795-5 2016 AgRP-stimulated ERK1/2 phosphorylation through MC3R was abolished by protein kinase A (PKA) inhibitor H-89 but not Rp-cAMPS, whereas AgRP-initiated ERK1/2 activation through MC4R was inhibited by phosphatidylinositol 3-kinase (PI3K) inhibitors wortmannin and LY294002. Wortmannin 244-254 mitogen-activated protein kinase 3 Homo sapiens 148-154 21056559-5 2011 Inhibition of the upstream regulators of Akt or ERK1/2, i.e. phosphoinositide 3-kinase (PI3K; using wortmannin or LY294002) or MEK1/2 (using UO126 or PD98509), abrogated the respective phosphorylation responses, and significantly impaired pro-survival activity. Wortmannin 100-110 mitogen-activated protein kinase 3 Homo sapiens 48-54 25034934-10 2014 ERK1/2 pathway can be blocked by the phosphoinositide-3 kinase inhibitor wortmannin. Wortmannin 73-83 mitogen-activated protein kinase 3 Homo sapiens 0-6 24617901-7 2014 Meanwhile, phosphorylation levels of ERK1/2 and Akt were partially blocked by ERK1/2 inhibitor (PD98059) and Akt inhibitor (wortmannin), respectively. Wortmannin 124-134 mitogen-activated protein kinase 3 Homo sapiens 37-43 22908970-10 2012 Specific ERK1/2 inhibitor PD98059 and PI3K inhibitor wortmannin reduced phosphorylation of ERK1/2 and Akt, respectively and blocked ghrelin- and des-acyl ghrelin-induced AGS cell proliferation. Wortmannin 53-63 mitogen-activated protein kinase 3 Homo sapiens 91-97 22809535-8 2013 Importantly, activation of ERK1/2, but not Akt, was induced by ghrelin in the newly-formed EBs, and the ghrelin-induced effects of cardiomyocyte differentiation were abolished by adding specific ERK1/2 inhibitor PD98059, but not specific PI3K inhibitor Wortmannin. Wortmannin 253-263 mitogen-activated protein kinase 3 Homo sapiens 27-33 22809535-8 2013 Importantly, activation of ERK1/2, but not Akt, was induced by ghrelin in the newly-formed EBs, and the ghrelin-induced effects of cardiomyocyte differentiation were abolished by adding specific ERK1/2 inhibitor PD98059, but not specific PI3K inhibitor Wortmannin. Wortmannin 253-263 mitogen-activated protein kinase 3 Homo sapiens 195-201 20506505-7 2010 Inhibitors of PI3K (LY294002 and Wortmannin) prevented the phosphorylation of ERK1/2, p38 MAPK, and Akt PI3K). Wortmannin 33-43 mitogen-activated protein kinase 3 Homo sapiens 78-84 20416296-4 2010 The icariin-induced eNOS phosphorylation was inhibited by wortmannin, a phosphatidylinositol 3-kinase (PI3K) inhibitor and partially attenuated by PD98059, an upstream inhibitor for ERK1/2. Wortmannin 58-68 mitogen-activated protein kinase 3 Homo sapiens 182-188