PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24059678-4 2013 In addition, while radiation-induced expression and phosphorylation of p53, inhibition of p53 function with pifithrin-alpha or transfection of cells with p53 siRNA significantly reduced the activation of both MMP2 and VEGF and resulted in a reduction of radiation-induced invasiveness. pifithrin 108-123 tumor protein p53 Homo sapiens 90-93 21887816-6 2013 Pretreatment with cytochrome P450 inhibitor alpha-naphthoflavone or p53 inhibitor pifithrin-alpha inhibited the benzo-[a]-pyrene-induced p21 expression. pifithrin 82-91 tumor protein p53 Homo sapiens 68-71 24179522-5 2013 The interaction between p53 and VEGF in the EGCG-treated cells was investigated using pifithrin-alpha. pifithrin 86-95 tumor protein p53 Homo sapiens 24-27 24059678-4 2013 In addition, while radiation-induced expression and phosphorylation of p53, inhibition of p53 function with pifithrin-alpha or transfection of cells with p53 siRNA significantly reduced the activation of both MMP2 and VEGF and resulted in a reduction of radiation-induced invasiveness. pifithrin 108-123 tumor protein p53 Homo sapiens 90-93 23648290-8 2013 A reduction by approximately 50% in the cytotoxicity of Gem and Clo was observed in the presence of pifithrin alpha, a p53 inhibitor. pifithrin 100-115 tumor protein p53 Homo sapiens 119-122 23652278-12 2013 An inhibitor of p53, pifithrin-alpha, attenuated the anticancer effects of OSU-03012 and downregulated the expression of Bax and cleaved caspase-9. pifithrin 21-30 tumor protein p53 Homo sapiens 16-19 22976055-5 2013 The p53 inhibitor pifithrin-alpha (PFT-alpha) knockdown of the signaling of p53 led vitexin to lose its antitumor effect and inhibited the expression of p53 downstream genes, p2(WAF1) and Bax. pifithrin 18-27 tumor protein p53 Homo sapiens 4-7 22976055-5 2013 The p53 inhibitor pifithrin-alpha (PFT-alpha) knockdown of the signaling of p53 led vitexin to lose its antitumor effect and inhibited the expression of p53 downstream genes, p2(WAF1) and Bax. pifithrin 18-27 tumor protein p53 Homo sapiens 76-79 22976055-5 2013 The p53 inhibitor pifithrin-alpha (PFT-alpha) knockdown of the signaling of p53 led vitexin to lose its antitumor effect and inhibited the expression of p53 downstream genes, p2(WAF1) and Bax. pifithrin 18-27 tumor protein p53 Homo sapiens 76-79 23640975-6 2013 In human HNSCC expressing PIK3CA and decreased wtTP53 and p73, PF-502 reciprocally enhanced TP53/p73 expression and growth inhibition, which was partially reversible by p53 inhibitor pifithrin-alpha. pifithrin 183-192 tumor protein p53 Homo sapiens 169-172 22983795-9 2013 Pretreatment with pifithrin-alpha, a p53 inhibitor, significantly diminished p53 phosphorylation at the concentration of 30 muM and abrogated quercetin-induced apoptosis in a dose-dependent manner. pifithrin 18-33 tumor protein p53 Homo sapiens 37-40 22983795-9 2013 Pretreatment with pifithrin-alpha, a p53 inhibitor, significantly diminished p53 phosphorylation at the concentration of 30 muM and abrogated quercetin-induced apoptosis in a dose-dependent manner. pifithrin 18-33 tumor protein p53 Homo sapiens 77-80 23816816-9 2013 These effects were absent when the caspase inhibitor z-VAD-fmk or p53 inhibitor PFTalpha were applied, suggesting that casticin could trigger cell apoptosis in a caspase-3 and p53-dependent manner. pifithrin 80-88 tumor protein p53 Homo sapiens 66-69 23201752-5 2013 Cell exposure to pifithrin, an inhibitor of p53 transcriptional activity, reduced the caspase activity associated with decreased miR-21 expression. pifithrin 17-26 tumor protein p53 Homo sapiens 44-47 22860893-5 2013 IL-24-mediated apoptosis was also antagonized by pifithrin-alpha, an inhibitor of p53 transactivation. pifithrin 49-64 tumor protein p53 Homo sapiens 82-85 23535401-8 2013 Furthermore, pretreatment with either p53 inhibitor pifithrin-alpha or calcium chelator BAPTA-AM effectively attenuated apoptosis induced by crotonaldehyde. pifithrin 52-67 tumor protein p53 Homo sapiens 38-41 23859040-5 2013 The presence of pifithrin, an inhibitor of p53 function, blocked ECG-induced apoptosis as was manifested by restored cell viability and caspase-3 activity to control values and reestablished the balance among Bcl-2 anti- and proapoptotic protein levels. pifithrin 16-25 tumor protein p53 Homo sapiens 43-46 23349842-6 2013 A p53 inhibitor pifithrin-alpha inhibited the expression of p21(Cip1) and attenuated homocysteine-induced cell growth arrest. pifithrin 16-31 tumor protein p53 Homo sapiens 2-5 21859809-4 2011 Moreover, MCP-1 upregulated p53 expression of HUVECs and the p53-specific inhibitor pifithrin-alpha (PFTalpha) rescued the MCP-1-induced apoptosis of HUVECs. pifithrin 84-99 tumor protein p53 Homo sapiens 61-64 25049533-1 2012 The aim of this study was to investigate the impact of a reported p53 inhibitor, pifithrin-alpha (PFT-alpha), on preimplantation porcine in vitro fertilized (IVF) embryo development in culture. pifithrin 81-90 tumor protein p53 Homo sapiens 66-69 22886373-8 2012 ROS-mediated p53 activation was found to involve in Cr(VI)-induced cell cycle arrest, and p53 inhibitor Pifithrin-alpha (PFT-alpha) rescued Cr(VI)-induced reduction of check point proteins Mrc1 and BubR1, thus inhibiting cell cycle arrest. pifithrin 104-113 tumor protein p53 Homo sapiens 90-93 22519734-10 2012 Furthermore, blocking p53-mediated transcription using pifithrin significantly reduced apoptosis. pifithrin 55-64 tumor protein p53 Homo sapiens 22-25 22445757-0 2012 The p53 inhibitor, pifithrin-alpha, suppresses self-renewal of embryonic stem cells. pifithrin 19-34 tumor protein p53 Homo sapiens 4-7 22445757-3 2012 In this study, we investigated the effect of pifithrin (PFT)-alpha, an inhibitor of p53-dependent transcriptional activation, on self-renewal of ES cells. pifithrin 45-54 tumor protein p53 Homo sapiens 84-87 22283740-5 2012 Pifithrin-alpha (PFT-alpha), a specific inhibitor of p53, reduced ROS production and reversed silibinin"s growth-inhibitory effect. pifithrin 0-15 tumor protein p53 Homo sapiens 53-56 23300887-13 2012 Inhibitor of p53, pifithrin-alpha, attenuated the anti-cancer effects of SDGE and apoptosis was also not observed in the p53(neg) SKOV-3 cells. pifithrin 18-33 tumor protein p53 Homo sapiens 13-16 22044530-5 2011 TCDD suppressed 1-NP- but not BaP-induced p53 activity, and in contrast, pifithrin-alpha (PFT-alpha), a p53 inhibitor, suppressed both 1-NP- and BaP-induced p53 activity. pifithrin 73-82 tumor protein p53 Homo sapiens 104-107 22044530-5 2011 TCDD suppressed 1-NP- but not BaP-induced p53 activity, and in contrast, pifithrin-alpha (PFT-alpha), a p53 inhibitor, suppressed both 1-NP- and BaP-induced p53 activity. pifithrin 73-82 tumor protein p53 Homo sapiens 104-107 21889606-0 2011 Study on X-ray-induced apoptosis and chromosomal damage in G2 human lymphocytes in the presence of pifithrin-alpha, an inhibitor of p53. pifithrin 99-114 tumor protein p53 Homo sapiens 132-135 21715570-7 2011 The p53 inhibitor pifithrin-alpha inhibited 3-NBA-induced apoptosis while small effects were seen using pifithrin-mu, suggesting that 3-NBA-induced cell death is a result of transcriptional activation of p53. pifithrin 18-33 tumor protein p53 Homo sapiens 4-7 21715570-7 2011 The p53 inhibitor pifithrin-alpha inhibited 3-NBA-induced apoptosis while small effects were seen using pifithrin-mu, suggesting that 3-NBA-induced cell death is a result of transcriptional activation of p53. pifithrin 18-33 tumor protein p53 Homo sapiens 204-207 22260024-8 2011 In addition, the cell growth inhibitory ratio and the apoptotic ratio of resveratrol-treated group decreased markedly by the p38 MAPK inhibitor SB203580 or p53 inhibitor pifithrin-alpha. pifithrin 170-185 tumor protein p53 Homo sapiens 156-159 22491426-5 2012 Furthermore, rRNA fragmentation was suppressed by the p53 inhibitors pifithrin-alpha and pifithrin-mu as well as the pan caspase inhibitor Z-VAD-FMK. pifithrin 69-78 tumor protein p53 Homo sapiens 54-57 22491426-5 2012 Furthermore, rRNA fragmentation was suppressed by the p53 inhibitors pifithrin-alpha and pifithrin-mu as well as the pan caspase inhibitor Z-VAD-FMK. pifithrin 89-98 tumor protein p53 Homo sapiens 54-57 22387547-3 2012 METHODS: Human hepatoma cells (HepG2.2.15) were transfected with XPD expression vector, followed by incubation with Pifithrin-alpha (P53 inhibitor). pifithrin 116-131 tumor protein p53 Homo sapiens 133-136 22387547-8 2012 However, the inhibition of P53 by Pifithrin-alpha abolished the above-mentioned effects of XPD. pifithrin 34-43 tumor protein p53 Homo sapiens 27-30 22815878-6 2012 In contrast, administration of pifithrin-alpha (a p53 activity inhibitor) during hypoxia resulted in protein levels that were similar to those of the control groups. pifithrin 31-46 tumor protein p53 Homo sapiens 50-53 21859809-4 2011 Moreover, MCP-1 upregulated p53 expression of HUVECs and the p53-specific inhibitor pifithrin-alpha (PFTalpha) rescued the MCP-1-induced apoptosis of HUVECs. pifithrin 101-109 tumor protein p53 Homo sapiens 61-64 21130866-8 2011 FasL up-regulation; activation of caspases-8, -2, -9, and -3; and chromatin condensation were decreased by the p53 inhibitor pifithrin-alpha, implicating p53 as an upstream factor in the activation of death receptor-mediated apoptosis by H(2)O(2). pifithrin 125-140 tumor protein p53 Homo sapiens 111-114 21130866-8 2011 FasL up-regulation; activation of caspases-8, -2, -9, and -3; and chromatin condensation were decreased by the p53 inhibitor pifithrin-alpha, implicating p53 as an upstream factor in the activation of death receptor-mediated apoptosis by H(2)O(2). pifithrin 125-140 tumor protein p53 Homo sapiens 154-157 21372157-3 2011 Moreover, the selective effect on erythropoiesis can be mimicked by activating p53 with the compound nutlin-3 and prevented by pifithrin-, an inhibitor of p53 activation. pifithrin 127-136 tumor protein p53 Homo sapiens 155-158 21324703-2 2011 In an attempt to identify novel p53 inhibitors, a quality collection of compounds structurally related to pifithrin-beta were designed and synthesized as potential inhibitors of p53. pifithrin 106-115 tumor protein p53 Homo sapiens 32-35 21324703-2 2011 In an attempt to identify novel p53 inhibitors, a quality collection of compounds structurally related to pifithrin-beta were designed and synthesized as potential inhibitors of p53. pifithrin 106-115 tumor protein p53 Homo sapiens 178-181 21163271-12 2011 p53-specific transcriptional inhibitor pifithrin-alpha or mitochondrial translocation inhibitor pifithrin-mu partially reversed solamargine-induced apoptosis. pifithrin 39-48 tumor protein p53 Homo sapiens 0-3 21212516-2 2011 Pifithrin-alpha (PFT-alpha), a specific inhibitor of p53, reduced autophagy and reversed silibinin"s growth-inhibitory effect; besides, PFT-alpha decreased the activation of caspase-3, a crucial executor of apoptosis. pifithrin 0-15 tumor protein p53 Homo sapiens 53-56 21266543-11 2011 The Pifithrin-alpha, an specific inhibitor of p53, inhibited the apoptosis and prevented the disruption of cytoskeleton induced by abnormal gravity. pifithrin 4-14 tumor protein p53 Homo sapiens 46-49 21274377-8 2011 We detected the effects of p53-mediated autophagy activation on the apoptosis of SGC7901 cells with the p53 inhibitor pifithrin-alpha. pifithrin 118-133 tumor protein p53 Homo sapiens 27-30 21274377-8 2011 We detected the effects of p53-mediated autophagy activation on the apoptosis of SGC7901 cells with the p53 inhibitor pifithrin-alpha. pifithrin 118-133 tumor protein p53 Homo sapiens 104-107 21274377-13 2011 SN50-induced increases in PUMA, DRAM, LC3 and Beclin 1 and cell death were blocked by the p53 specific inhibitor pifithrin-alpha. pifithrin 113-128 tumor protein p53 Homo sapiens 90-93 22041910-0 2011 High dose of pyridoxine induces IGFBP-3 mRNA expression in MCF-7 cells and its induction is inhibited by the p53-specific inhibitor pifithrin-alpha. pifithrin 132-141 tumor protein p53 Homo sapiens 109-112 22041910-7 2011 The induction of IGFBP-3 by PN was inhibited by a p53-specific inhibitor, pifithrin-alpha, in a dose-dependent manner, but was not affected by PD169316 (MAPK inhibitor), AS601245 (c-Jun N-terminal kinase inhibitor) or SL327 (MEK1/2 inhibitor). pifithrin 74-89 tumor protein p53 Homo sapiens 50-53 20708607-11 2010 Inhibition of p53 expression by a specific p53 inhibitor pifithrin-alpha reduced the phosphorylated p38 MAP kinase and active caspase-3 proteins in the oxaliplatin-exposed RKO cells. pifithrin 57-66 tumor protein p53 Homo sapiens 14-17 20708607-11 2010 Inhibition of p53 expression by a specific p53 inhibitor pifithrin-alpha reduced the phosphorylated p38 MAP kinase and active caspase-3 proteins in the oxaliplatin-exposed RKO cells. pifithrin 57-66 tumor protein p53 Homo sapiens 43-46 20947454-5 2010 In accordance with the dependence of ActD-induced apoptosis on a mitochondrial p53 function, in control T-cells specific inhibition of mitochondrial p53 translocation with mu pifithrin reduced apoptosis by 86%, whereas treatment with alpha pifithrin, which blocks p53-mediated transcription, had no effect. pifithrin 175-184 tumor protein p53 Homo sapiens 149-152 20947454-5 2010 In accordance with the dependence of ActD-induced apoptosis on a mitochondrial p53 function, in control T-cells specific inhibition of mitochondrial p53 translocation with mu pifithrin reduced apoptosis by 86%, whereas treatment with alpha pifithrin, which blocks p53-mediated transcription, had no effect. pifithrin 175-184 tumor protein p53 Homo sapiens 149-152 20348016-0 2010 Relation between DNA repair, apoptosis and chromosomal aberrations in presence of pifithrin-alpha, an inhibitor of p53. pifithrin 82-97 tumor protein p53 Homo sapiens 115-118 20718603-2 2010 Pifithrin-alpha (PFT-alpha), a specific inhibitor of p53, has been suggested as a combinatory agent in the treatment of p53-deficient tumors in which inhibition of p53 would not compromise therapeutic efficacy but would decrease p53-mediated side effects in normal tissue. pifithrin 0-10 tumor protein p53 Homo sapiens 53-56 20718603-2 2010 Pifithrin-alpha (PFT-alpha), a specific inhibitor of p53, has been suggested as a combinatory agent in the treatment of p53-deficient tumors in which inhibition of p53 would not compromise therapeutic efficacy but would decrease p53-mediated side effects in normal tissue. pifithrin 0-10 tumor protein p53 Homo sapiens 120-123 20718603-2 2010 Pifithrin-alpha (PFT-alpha), a specific inhibitor of p53, has been suggested as a combinatory agent in the treatment of p53-deficient tumors in which inhibition of p53 would not compromise therapeutic efficacy but would decrease p53-mediated side effects in normal tissue. pifithrin 0-10 tumor protein p53 Homo sapiens 120-123 20307555-5 2010 This RAR could be slightly reduced by pifithrin-alpha, an inhibitor of P53, however it was completely suppressed by BAY11-7082, an inhibitor of nuclear factor-kappaB (NF-kappaB). pifithrin 38-53 tumor protein p53 Homo sapiens 71-74 19955567-6 2010 Furthermore, we characterize for the first time the binding behavior of Pifithrin-mu, a specific small molecule inhibitor of the p53-BclxL interaction, and present a structural model of the protein-ligand complex. pifithrin 72-81 tumor protein p53 Homo sapiens 129-132 20393600-10 2010 FasL upregulation and caspase-8 activation were decreased by p53 inhibitor pifithrin-alpha and antioxidant polyethylene glycol catalase. pifithrin 75-90 tumor protein p53 Homo sapiens 61-64 20036849-3 2010 Co-treatment of cells with SP600125 and p53 inhibitor, pifithrin-alpha, partially nullified the suppressive effects of SP610025 on PS1 expression and secreted Abeta40 level. pifithrin 55-64 tumor protein p53 Homo sapiens 40-43 19895660-7 2010 The p53 transcription inhibitor, pifithrin-alpha, (10 microM) significantly reduced the expression of p53 in microglia and significantly modulated the levels of microglial apoptosis induced by activation. pifithrin 33-48 tumor protein p53 Homo sapiens 4-7 19895660-7 2010 The p53 transcription inhibitor, pifithrin-alpha, (10 microM) significantly reduced the expression of p53 in microglia and significantly modulated the levels of microglial apoptosis induced by activation. pifithrin 33-48 tumor protein p53 Homo sapiens 102-105 19812371-11 2010 In U87MG (p53wt) cells cotreated with TPT and pifithrin-alpha, or transfected with p53-siRNA, caspase-2 and Bid were significantly cleaved and XIAP/survivin was degraded. pifithrin 46-55 tumor protein p53 Homo sapiens 10-13 21071999-0 2010 Pifithrin-alpha, an inhibitor of p53 transactivation, up-regulates COX-2 expression through an MAPK-dependent pathway. pifithrin 0-15 tumor protein p53 Homo sapiens 33-36 21071999-2 2010 In this study, we investigated the effect of pifithrin (PFT)-alpha, an inhibitor of p53 transactivation, on COX-2 expression in breast and fibrosarcoma cells. pifithrin 45-54 tumor protein p53 Homo sapiens 84-87 19184017-7 2009 Importantly, pifithrin-alpha, a p53 inhibitor, protected against DOX-induced mitochondrial depolarization, caspase activation and cell death. pifithrin 13-22 tumor protein p53 Homo sapiens 32-35 19646415-4 2009 Inhibition of NF-kappaB or p53 activation by its specific inhibitor PDTC or pifithrin-alpha respectively, significantly reduced both oridonin-induced apoptosis and autophagy accompanied by the decrease in Beclin 1 and LC3 levels. pifithrin 76-91 tumor protein p53 Homo sapiens 27-30 19541794-8 2009 Cells pretreatment with the p53 inhibitor pifithrin-alpha suggested that ZEN but not T-2 toxin triggered a p53-dependent mitochondrial apoptotic pathway. pifithrin 42-51 tumor protein p53 Homo sapiens 28-31 19706164-5 2009 Inhibition of p53 was achieved in U87MG cells transfected with E6 oncoprotein (U87MG-E6) and treated with pifithrin-alpha, a reversible inhibitor of p53 (U87MG-PFT). pifithrin 106-121 tumor protein p53 Homo sapiens 14-17 19706164-5 2009 Inhibition of p53 was achieved in U87MG cells transfected with E6 oncoprotein (U87MG-E6) and treated with pifithrin-alpha, a reversible inhibitor of p53 (U87MG-PFT). pifithrin 106-121 tumor protein p53 Homo sapiens 149-152 19541794-8 2009 Cells pretreatment with the p53 inhibitor pifithrin-alpha suggested that ZEN but not T-2 toxin triggered a p53-dependent mitochondrial apoptotic pathway. pifithrin 42-51 tumor protein p53 Homo sapiens 107-110 19513548-10 2009 The p53 inhibitor, pifithrin-alpha, was able to counteract the effect of Axin in C6 cells. pifithrin 19-34 tumor protein p53 Homo sapiens 4-7 19347880-4 2009 Treatment with a p53 specific inhibitor, pifithrin alpha, restored this resistant phenotype in the MCF-7 cell line. pifithrin 41-50 tumor protein p53 Homo sapiens 17-20 19428812-6 2009 Pifithrin-alpha (PFT), an inhibitor of p53, blocked these processes. pifithrin 0-15 tumor protein p53 Homo sapiens 39-42 19428812-6 2009 Pifithrin-alpha (PFT), an inhibitor of p53, blocked these processes. pifithrin 17-20 tumor protein p53 Homo sapiens 39-42 18834919-7 2008 HepG2 cell treatment with the p53 inhibitor pifithrin-alpha (PFT) and western blot analysis suggested that both ZEN and OTA, but not T-2 toxin, trigger a p53-dependent apoptotic pathway. pifithrin 44-59 tumor protein p53 Homo sapiens 30-33 19411846-9 2009 Specific inhibition of mitochondrial p53 translocation by Pifithrin mu reduces the apoptotic Nutlin response by 2.5-fold, underlining the significance of p53"s mitochondrial program in Nutlin-induced apoptosis. pifithrin 58-67 tumor protein p53 Homo sapiens 37-40 18459128-4 2009 Further, the treatment of A549 cells with pifithrin-alpha, a specific inhibitor of p53, or transfection of A549 cells with a p53 antisense oligodeoxynucleotide resulted in a reduction in the berberine-induced inhibition of cell proliferation and apoptosis. pifithrin 42-51 tumor protein p53 Homo sapiens 83-86 18459128-4 2009 Further, the treatment of A549 cells with pifithrin-alpha, a specific inhibitor of p53, or transfection of A549 cells with a p53 antisense oligodeoxynucleotide resulted in a reduction in the berberine-induced inhibition of cell proliferation and apoptosis. pifithrin 42-51 tumor protein p53 Homo sapiens 125-128 19088112-2 2009 Male germ cell apoptosis was initiated with doses as low as 0.1 Gy and was prevented by pifithrin alpha, a p53 inhibitor. pifithrin 88-103 tumor protein p53 Homo sapiens 107-110 19088112-7 2009 Inhibition of p53 with pifithrin alpha did not affect oogonia apoptosis following irradiation. pifithrin 23-38 tumor protein p53 Homo sapiens 14-17 19267667-1 2009 Pifithrin alpha (PFTalpha), one of the first known low molecular weight modulators of activity of tumor suppressor p53, increases survival of hemopoietic clonogenic cells (evaluated by the criterion of formation of endogenous spleen CFU-C8 colonies in irradiated animals). pifithrin 0-15 tumor protein p53 Homo sapiens 115-118 19267667-1 2009 Pifithrin alpha (PFTalpha), one of the first known low molecular weight modulators of activity of tumor suppressor p53, increases survival of hemopoietic clonogenic cells (evaluated by the criterion of formation of endogenous spleen CFU-C8 colonies in irradiated animals). pifithrin 17-25 tumor protein p53 Homo sapiens 115-118 18834919-7 2008 HepG2 cell treatment with the p53 inhibitor pifithrin-alpha (PFT) and western blot analysis suggested that both ZEN and OTA, but not T-2 toxin, trigger a p53-dependent apoptotic pathway. pifithrin 44-59 tumor protein p53 Homo sapiens 154-157 18547707-10 2008 Pifithrin alpha, a p53 transcriptional inhibitor, increases Cr (VI) cytotoxicity, suggesting a role of p53 as a survival molecule. pifithrin 0-15 tumor protein p53 Homo sapiens 19-22 18813780-11 2008 Incubation with either the irreversible pan-caspase inhibitors Z-VAD-FMK, or SP600125, a selective inhibitor of JNK, or pifithrin alpha, a potent p53 inhibitor, significantly inhibited the effects induced by PTX. pifithrin 120-135 tumor protein p53 Homo sapiens 146-149 18628404-7 2008 Pifithrin-alpha, an inhibitor of the tumor suppressor protein p53, had no effect on ROS generation but did block alpha1D-AR-induced apoptosis. pifithrin 0-15 tumor protein p53 Homo sapiens 62-65 18847491-11 2008 Pretreatment of cells with pifithrin-alpha or U0126, specific inhibitors of p53 or MEK-1/2, significantly attenuated Triphala-induced apoptosis. pifithrin 27-42 tumor protein p53 Homo sapiens 76-79 17960385-10 2008 An inhibitor of p53, pifithrin-alpha, antagonized the expression of the cleaved form of caspase-3 in MKN45 cells. pifithrin 21-30 tumor protein p53 Homo sapiens 16-19 18547707-10 2008 Pifithrin alpha, a p53 transcriptional inhibitor, increases Cr (VI) cytotoxicity, suggesting a role of p53 as a survival molecule. pifithrin 0-15 tumor protein p53 Homo sapiens 103-106 18448277-5 2008 Inhibition of p53 by either its inhibitor pifithrin-alpha or p53 siRNA markedly increased B(a)P-induced the activation of c-Jun, Akt and ERK in this context. pifithrin 42-51 tumor protein p53 Homo sapiens 14-17 18084327-4 2008 Interestingly, more pronounced tumoricidal effects were observed in p53-deficient HCT116 and HT29 cells, as well as A549 cells treated with the p53 inhibitor cyclic pifithrin-alpha. pifithrin 165-180 tumor protein p53 Homo sapiens 68-71 18084327-4 2008 Interestingly, more pronounced tumoricidal effects were observed in p53-deficient HCT116 and HT29 cells, as well as A549 cells treated with the p53 inhibitor cyclic pifithrin-alpha. pifithrin 165-180 tumor protein p53 Homo sapiens 144-147 18342637-6 2008 All these effects were significantly blocked by pretreatment of cells with the antioxidant N-acetylcysteine, p53 inhibitor pifithrin-alpha, and enzyme catalase. pifithrin 123-138 tumor protein p53 Homo sapiens 109-112 18374905-15 2008 Furthermore, p53 inhibitor pifithrin-alpha partially nullified the suppressive effects of SP600125 on PS1 expression. pifithrin 27-42 tumor protein p53 Homo sapiens 13-16 17855337-6 2007 Furthermore, RNA interference-mediated lowering of the p53 protein in T1 cells or pifithrin-alpha-induced p53-specific inhibition activity significantly decreased CTL-induced target killing mediated by CTL or recombinant GrB. pifithrin 82-91 tumor protein p53 Homo sapiens 106-109 17965848-5 2008 Consequential decrease in cell viability was in part prevented by the p53 inhibitor pifithrin-alpha, suggesting that p53 translocation contributes to QD-induced cytotoxicity. pifithrin 84-99 tumor protein p53 Homo sapiens 70-73 17965848-5 2008 Consequential decrease in cell viability was in part prevented by the p53 inhibitor pifithrin-alpha, suggesting that p53 translocation contributes to QD-induced cytotoxicity. pifithrin 84-99 tumor protein p53 Homo sapiens 117-120 18344606-4 2008 Pifithrin-alpha, a p53 inhibitor, significantly prevented trimidox-induced apoptotic characteristics, as detected by nuclear morphological observation and DNA fragmentation. pifithrin 0-15 tumor protein p53 Homo sapiens 19-22 17846083-5 2008 Apoptosis was prevented by the p53 inhibitor pifithrin and by p53 antisense oligonucleotides, indicating dependency of force-induced apoptosis on p53. pifithrin 45-54 tumor protein p53 Homo sapiens 31-34 18097557-5 2008 Experiments performed using pifithrin-alpha, a reversible inhibitor of p53, showed a correlation between acetylation of p53 and induction of apoptosis. pifithrin 28-43 tumor protein p53 Homo sapiens 71-74 18097557-5 2008 Experiments performed using pifithrin-alpha, a reversible inhibitor of p53, showed a correlation between acetylation of p53 and induction of apoptosis. pifithrin 28-43 tumor protein p53 Homo sapiens 120-123 17673346-2 2007 The aim of this study was to investigate the effect of pifithrin-alpha (PFTalpha; a specific p53 inhibitor) and mitogen-activated protein kinases (MAPKs) inhibitors [namely SP600125 (a specific JNK inhibitor), SB203580 (a specific p38 inhibitor) and PD98059 (a specific ERK inhibitor)] on apoptotic signaling transduction mechanism induced by Cin in human hepatoma PLC/PRF/5 (CD95-negative) cells. pifithrin 72-80 tumor protein p53 Homo sapiens 93-96 17855051-3 2007 In this study, we show that Bax translocation, caspase-3 activation and cell death by UV irradiation are not affected by Z-IETD-fmk (caspase-8 inhibitor), but delayed by Pifithrin-alpha (p53 inhibitor), although Bid cleavage could be completely abolished by Z-IETD-fmk. pifithrin 170-179 tumor protein p53 Homo sapiens 187-190 17855337-9 2007 Treatment of T1 cells with pifithrin-alpha resulted in inhibition of p53 phosphorylation at these residues and in a significant decrease in GrB-induced apoptotic T1 cell death. pifithrin 27-36 tumor protein p53 Homo sapiens 69-72 17968428-6 2007 To verify the role of p53, the assays were repeated in presence of pifithrin-alpha, an inhibitor of p53. pifithrin 67-82 tumor protein p53 Homo sapiens 100-103 17340621-11 2007 In addition, inhibition of CD95/Fas receptor on the cell surface of MCF-EGFP cells by treatment with Pifithrin-alpha, a p53 specific transactivation inhibitor, partially abrogated the sensitivity of bystander MCF-EGFP cells. pifithrin 101-116 tumor protein p53 Homo sapiens 120-123 17630850-5 2007 The p53 inhibitor pifithrin did not block GSNO-induced cell death. pifithrin 18-27 tumor protein p53 Homo sapiens 4-7 17456577-13 2007 Finally, when we added pifithrin-alpha, a specific inhibitor of p53 functions, a significant decrease in irradiation-induced apoptosis in both germ and Sertoli cells was observed, indicating the involvement of the p53 pathway in irradiation-induced apoptosis. pifithrin 23-38 tumor protein p53 Homo sapiens 64-67 17456577-13 2007 Finally, when we added pifithrin-alpha, a specific inhibitor of p53 functions, a significant decrease in irradiation-induced apoptosis in both germ and Sertoli cells was observed, indicating the involvement of the p53 pathway in irradiation-induced apoptosis. pifithrin 23-38 tumor protein p53 Homo sapiens 214-217 17505786-5 2007 Moreover, the presence of pifithrin-alpha, an inhibitor of p53 transcriptional activity, blocked cisplatin-induced apoptosis, reduced the generation of ROS produced upon cisplatin treatment. pifithrin 26-41 tumor protein p53 Homo sapiens 59-62 17341629-6 2006 Pifithrin-alpha (PFT), an inhibitor of p53 transcriptional activity, protected only NB4 cells with functional p53 from FK228-induced apoptosis and did not interfere with antiproliferative activity in p53-negative HL-60 cells. pifithrin 0-15 tumor protein p53 Homo sapiens 39-42 16936710-6 2007 In addition, the increased expression of DR5 receptor after beta amyloid treatment was sustained by p53 transcriptional activity, as demonstrated by the data showing that the p53 inhibitor Pifithrin alpha prevented both beta amyloid-induced DR5 induction and cell death. pifithrin 189-204 tumor protein p53 Homo sapiens 100-103 16936710-6 2007 In addition, the increased expression of DR5 receptor after beta amyloid treatment was sustained by p53 transcriptional activity, as demonstrated by the data showing that the p53 inhibitor Pifithrin alpha prevented both beta amyloid-induced DR5 induction and cell death. pifithrin 189-204 tumor protein p53 Homo sapiens 175-178 16902184-0 2007 Pifithrin-alpha, an inhibitor of p53 transactivation, alters the inflammatory process and delays tendon healing following acute injury. pifithrin 0-15 tumor protein p53 Homo sapiens 33-36 16902184-2 2007 The goal of this study was to evaluate the effect of a p53 transactivation inhibitor, namely, pifithrin-alpha, on the pathophysiological sequence following collagenase-induced tendon injury. pifithrin 94-109 tumor protein p53 Homo sapiens 55-58 17341629-6 2006 Pifithrin-alpha (PFT), an inhibitor of p53 transcriptional activity, protected only NB4 cells with functional p53 from FK228-induced apoptosis and did not interfere with antiproliferative activity in p53-negative HL-60 cells. pifithrin 0-15 tumor protein p53 Homo sapiens 110-113 17341629-6 2006 Pifithrin-alpha (PFT), an inhibitor of p53 transcriptional activity, protected only NB4 cells with functional p53 from FK228-induced apoptosis and did not interfere with antiproliferative activity in p53-negative HL-60 cells. pifithrin 0-15 tumor protein p53 Homo sapiens 110-113 16971506-7 2006 The p53 inhibitor pifithrin-alpha protects HepG2 cells against apoptosis induced by serum withdrawal. pifithrin 18-33 tumor protein p53 Homo sapiens 4-7 16982703-10 2006 Pifithrin-alpha enhanced chemosensitivity by a mechanism independent of p53 and involving AhR and p38 MAPK deregulation of eIF-4E phosphorylation. pifithrin 0-9 tumor protein p53 Homo sapiens 72-75 16135398-5 2006 Moreover, TPL inhibited the clonogenic growth of MCF 7 cells, which was significantly recovered by pifithrin-alpha, the p53 inhibitor. pifithrin 99-114 tumor protein p53 Homo sapiens 120-123 16777994-5 2006 Pifithrin-alpha, a p53 inhibitor, reversed silibinin-induced caspase activation including caspase 2; however, caspase 2 inhibitor also reversed p53 phosphorylation suggesting a bidirectional regulation between them. pifithrin 0-15 tumor protein p53 Homo sapiens 19-22 16844116-6 2006 Pretreatment with p53 inhibitor pifithrin alpha cancelled the IR-induced downregulation of FAP-1 mRNA. pifithrin 32-41 tumor protein p53 Homo sapiens 18-21 16204064-7 2005 Treatment of p53 wild-type cells with pifithrin-alpha, an inhibitor of the trans-activating activity of p53, caused reversal of the phenotype, making wild-type cells more sensitive to topotecan. pifithrin 38-53 tumor protein p53 Homo sapiens 13-16 17065086-1 2006 Previously, we have found that activation of deoxycytidine kinase elicited by various DNA-damaging chemical agents could be prevented by BAPTA-AM, a cell-permeable calcium chelator or by pifithrin-alpha, a pharmacological inhibitor of p53. pifithrin 187-202 tumor protein p53 Homo sapiens 235-238 16778187-8 2006 The p53 inhibitor, pifithrin-alpha, is able to attenuate the effect of proteasome inhibition. pifithrin 19-34 tumor protein p53 Homo sapiens 4-7 16288207-9 2006 Pretreatment with the specific p53 inhibitor pifithrin or downregulation of p53 using a specific small inhibitory RNA significantly attenuated ODQ-induced apoptosis. pifithrin 45-54 tumor protein p53 Homo sapiens 31-34 16467109-7 2006 Radiosensitization of cells expressing p53 by TSA was reduced by pifithrin-alpha, a small-molecule inhibitor of p53. pifithrin 65-80 tumor protein p53 Homo sapiens 39-42 16467109-7 2006 Radiosensitization of cells expressing p53 by TSA was reduced by pifithrin-alpha, a small-molecule inhibitor of p53. pifithrin 65-80 tumor protein p53 Homo sapiens 112-115 16204041-8 2005 Intact global NER was also confirmed in SCC25 cells possessing inactivating mutations in p53 as well as in cells treated with pifithrin-alpha, a chemical inhibitor of p53 that decreased sensitivity of cells to UV radiation. pifithrin 126-141 tumor protein p53 Homo sapiens 167-170 16204064-7 2005 Treatment of p53 wild-type cells with pifithrin-alpha, an inhibitor of the trans-activating activity of p53, caused reversal of the phenotype, making wild-type cells more sensitive to topotecan. pifithrin 38-53 tumor protein p53 Homo sapiens 104-107 15983031-4 2005 Pancaspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone or p53 inhibitor pifithrin-alpha markedly prevented mitochondrial release of AIF, suggesting that caspases and p53 are involved in this release. pifithrin 87-102 tumor protein p53 Homo sapiens 73-76 15983031-4 2005 Pancaspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone or p53 inhibitor pifithrin-alpha markedly prevented mitochondrial release of AIF, suggesting that caspases and p53 are involved in this release. pifithrin 87-102 tumor protein p53 Homo sapiens 181-184 15983031-10 2005 The p53 inhibitor pifithrin-alpha or p53 siRNA prevented both cisplatin-induced caspase-2 activation and mitochondrial release of AIF. pifithrin 18-27 tumor protein p53 Homo sapiens 4-7 16170037-7 2005 The use of the chemical inhibitor of p53, pifithrin alpha, excluded this possibility. pifithrin 42-51 tumor protein p53 Homo sapiens 37-40 16005638-6 2005 Moreover, gp120 neurotoxicity is markedly reduced by the p53-inhibitor, pifithrin-alpha. pifithrin 72-87 tumor protein p53 Homo sapiens 57-60 15548639-9 2005 Pifithrin-alpha, a synthetic inhibitor of p53 activity, abolished both S-phase arrest and apoptosis induced by genotoxic PAHs, and it potentiated the proliferative effect of BaP. pifithrin 0-15 tumor protein p53 Homo sapiens 42-45 15976193-6 2005 The p53 inhibitor PFTalpha reduced both DON-induced phosphorylation of p53 and p53 binding activity. pifithrin 18-26 tumor protein p53 Homo sapiens 4-7 15976193-6 2005 The p53 inhibitor PFTalpha reduced both DON-induced phosphorylation of p53 and p53 binding activity. pifithrin 18-26 tumor protein p53 Homo sapiens 71-74 15976193-6 2005 The p53 inhibitor PFTalpha reduced both DON-induced phosphorylation of p53 and p53 binding activity. pifithrin 18-26 tumor protein p53 Homo sapiens 71-74 15865929-3 2005 This strategy was validated by isolation and testing of small molecule p53 inhibitor pifithrin-alpha that demonstrated broad tissue protecting capacity. pifithrin 85-100 tumor protein p53 Homo sapiens 71-74 15800902-1 2005 Pifithrin-alpha (PFTalpha) is a small molecule inhibitor of p53. pifithrin 0-15 tumor protein p53 Homo sapiens 60-63 15800902-1 2005 Pifithrin-alpha (PFTalpha) is a small molecule inhibitor of p53. pifithrin 17-25 tumor protein p53 Homo sapiens 60-63 15800902-5 2005 We observed that exposure of U87MG cells to PFTalpha before cytotoxic chemotherapy attenuated p53-mediated induction of p21WAF1 protein levels, sensitizing U87MG cells to BCNU and TMZ. pifithrin 44-52 tumor protein p53 Homo sapiens 94-97 15800902-7 2005 Our findings suggest that in addition to protecting normal cells from the toxic effects of radiation and chemotherapy, small molecule inhibitors of p53, like PFTalpha, might play a role in clinical oncology by sensitizing certain resistant cancers to cytotoxic chemotherapies. pifithrin 158-166 tumor protein p53 Homo sapiens 148-151 15843497-0 2005 The p53 inhibitor pifithrin-alpha is a potent agonist of the aryl hydrocarbon receptor. pifithrin 18-33 tumor protein p53 Homo sapiens 4-7 15547111-7 2005 Co-incubation with pifithrin-alpha (PFT-alpha), a selective p53 inhibitor, restored GADD45, Bcl2, cyclin B1, and p21(WAF1) expression levels and almost completely reversed the growth inhibitory, cell cycle, and apoptotic effects of 5-aza-CR. pifithrin 19-28 tumor protein p53 Homo sapiens 60-63 15331571-6 2004 Treatment of nonluteinized macaque granulosa cells with hCG and the p53 inhibitor pifithrin-alpha (PFT) in vitro did not alter markers of the cell cycle, including proliferating cell nuclear antigen, p21, and human double minute (HDM)-2 expression compared with hCG alone. pifithrin 82-97 tumor protein p53 Homo sapiens 68-71 15498930-8 2004 Furthermore, the low-dose hypersensitivity and Annexin V binding to irradiated A549 and T98G cells were eliminated by treating the cells with pifithrin, an inhibitor of p53. pifithrin 142-151 tumor protein p53 Homo sapiens 169-172 15378026-7 2004 Activation of endogenous wt p53 in BLs and lymphoblastoid cell lines led to the induction of SAP and this was inhibited by the specific p53 inhibitor pifithrin-alpha. pifithrin 150-165 tumor protein p53 Homo sapiens 28-31 15378026-7 2004 Activation of endogenous wt p53 in BLs and lymphoblastoid cell lines led to the induction of SAP and this was inhibited by the specific p53 inhibitor pifithrin-alpha. pifithrin 150-165 tumor protein p53 Homo sapiens 136-139 15145929-1 2004 Pifithrin-alpha (PFTalpha) was originally thought to be a specific inhibitor of signaling by the tumor suppressor protein p53. pifithrin 0-15 tumor protein p53 Homo sapiens 122-125 15302096-7 2004 Pifithrin-alpha, a chemical inhibitor of wild-type p53, ameliorated the cytotoxicity of Ara-C in NALM-6 and MOLT-4, but not Jurkat, U937 or HL-60. pifithrin 0-15 tumor protein p53 Homo sapiens 51-54 15145929-1 2004 Pifithrin-alpha (PFTalpha) was originally thought to be a specific inhibitor of signaling by the tumor suppressor protein p53. pifithrin 17-25 tumor protein p53 Homo sapiens 122-125 15144874-0 2004 The role of p53-induced apoptosis in cerebral ischemia: effects of the p53 inhibitor pifithrin alpha. pifithrin 85-100 tumor protein p53 Homo sapiens 71-74 15252149-10 2004 Attenuating p53 by a chemical inhibitor pifithrin-alpha decreased the selenite-induced p53 Ser15P and led to concordant reductions of PARP cleavage and apoptosis. pifithrin 40-49 tumor protein p53 Homo sapiens 12-15 15252149-10 2004 Attenuating p53 by a chemical inhibitor pifithrin-alpha decreased the selenite-induced p53 Ser15P and led to concordant reductions of PARP cleavage and apoptosis. pifithrin 40-49 tumor protein p53 Homo sapiens 87-90 15054096-8 2004 Further evidence was obtained using a p53 inhibitor, pifithrin-alpha. pifithrin 53-68 tumor protein p53 Homo sapiens 38-41 15054096-9 2004 Pifithrin-alpha completely suppressed DOX-induced activation of p53 in both normal and tumor cell lines and prevented apoptosis in tumor cell lines but not in endothelial cells and cardiomyocytes. pifithrin 0-15 tumor protein p53 Homo sapiens 64-67 15144874-4 2004 Pifithrin alpha reduced the number of apoptotic cells in the ischemic brain by inhibiting the binding of p53 to its DNA sites as it reduced the expression of the p53-related gene p21(WAF) without changing the amount of p53 protein itself. pifithrin 0-15 tumor protein p53 Homo sapiens 105-108 15144874-4 2004 Pifithrin alpha reduced the number of apoptotic cells in the ischemic brain by inhibiting the binding of p53 to its DNA sites as it reduced the expression of the p53-related gene p21(WAF) without changing the amount of p53 protein itself. pifithrin 0-15 tumor protein p53 Homo sapiens 162-165 15144874-4 2004 Pifithrin alpha reduced the number of apoptotic cells in the ischemic brain by inhibiting the binding of p53 to its DNA sites as it reduced the expression of the p53-related gene p21(WAF) without changing the amount of p53 protein itself. pifithrin 0-15 tumor protein p53 Homo sapiens 162-165 14993250-6 2004 Microarrays revealed that most (85%) of the transcriptional effects of HIV-1 Env were blocked by the p53 inhibitor pifithrin-alpha. pifithrin 115-130 tumor protein p53 Homo sapiens 101-104 15001399-6 2004 In contrast, in MCF-7 cells with wild type p53 and estrogen receptor, there was no change in Akt activation, while suppression of p53 activity by pifithrin-alpha increased phosphorylation of Akt after the treatment with copper. pifithrin 146-155 tumor protein p53 Homo sapiens 130-133 12967348-0 2003 The p53-inhibitor pifithrin-alpha inhibits firefly luciferase activity in vivo and in vitro. pifithrin 18-33 tumor protein p53 Homo sapiens 4-7 12967348-2 2003 It has been proposed that the use of pifithrin-alpha in conjunction with chemotherapeutic and radiation therapies for cancer will reduce the side effects of these treatments in normal tissue that still contains wild type p53. pifithrin 37-46 tumor protein p53 Homo sapiens 221-224 12967348-3 2003 In addition, pifithrin-alpha provides a useful tool in the laboratory to investigate the function of p53 in model systems. pifithrin 13-22 tumor protein p53 Homo sapiens 101-104 12884365-1 2003 Recently, blockage of p53-dependent transcriptional activation and apoptosis by pifithrin-alpha (PFTalpha) has been reported to be useful for reducing the side effects of cancer therapy and the compound is thus thought to be a specific inhibitor of p53 [Komarov et al., Science 1999;285:1733-1737]. pifithrin 80-95 tumor protein p53 Homo sapiens 22-25 12893085-0 2003 Inhibition of p53, p21 and Bax by pifithrin-alpha does not affect UV induced apoptotic response in CS-B cells. pifithrin 34-49 tumor protein p53 Homo sapiens 14-17 12884365-6 2003 Thus, we conclude that PFTalpha is not a specific p53 inhibitor in JB6 cells but is a potential activator of p53-mediated signaling and apoptosis. pifithrin 23-31 tumor protein p53 Homo sapiens 109-112 12884365-1 2003 Recently, blockage of p53-dependent transcriptional activation and apoptosis by pifithrin-alpha (PFTalpha) has been reported to be useful for reducing the side effects of cancer therapy and the compound is thus thought to be a specific inhibitor of p53 [Komarov et al., Science 1999;285:1733-1737]. pifithrin 80-95 tumor protein p53 Homo sapiens 249-252 12884365-1 2003 Recently, blockage of p53-dependent transcriptional activation and apoptosis by pifithrin-alpha (PFTalpha) has been reported to be useful for reducing the side effects of cancer therapy and the compound is thus thought to be a specific inhibitor of p53 [Komarov et al., Science 1999;285:1733-1737]. pifithrin 97-105 tumor protein p53 Homo sapiens 22-25 12884365-1 2003 Recently, blockage of p53-dependent transcriptional activation and apoptosis by pifithrin-alpha (PFTalpha) has been reported to be useful for reducing the side effects of cancer therapy and the compound is thus thought to be a specific inhibitor of p53 [Komarov et al., Science 1999;285:1733-1737]. pifithrin 97-105 tumor protein p53 Homo sapiens 249-252 12884365-3 2003 Instead, p53-dependent activation and apoptosis were not only induced by PFTalpha itself but were also enhanced by a combination of PFTalpha with UVB or Dox. pifithrin 73-81 tumor protein p53 Homo sapiens 9-12 12884365-4 2003 Furthermore, PFTalpha-induced apoptosis was mediated through p53-dependent and -independent signaling pathways. pifithrin 13-21 tumor protein p53 Homo sapiens 61-64 12884365-5 2003 Extracellular signal-regulated kinases and p38 kinase, but not c-jun N-terminal kinases (JNKs), were activated, and these activations were required for phosphorylation and accumulation of p53 in the cellular apoptotic response to PFTalpha. pifithrin 230-238 tumor protein p53 Homo sapiens 188-191 12556443-8 2003 Interestingly, CTF-PS2-induced caspase 3 activation is prevented by pifithrin-alpha, a selective blocker of p53 transcriptional activity. pifithrin 68-77 tumor protein p53 Homo sapiens 108-111 12131363-13 2002 Pifithrin-alpha (Calbiochem), a p53 inhibitor, blocked resveratrol induced serine-15 phosphorylation of p53 and p53 binding to DNA. pifithrin 0-15 tumor protein p53 Homo sapiens 32-35 12660163-5 2003 Induction of ICAM-1 is abolished after treatment with the specific p53 inhibitor pifithrin-alpha and is abrogated in p53-deficient cell lines. pifithrin 81-96 tumor protein p53 Homo sapiens 67-70 12151395-5 2002 Moreover, the role of p53 in activation of this pathway was demonstrated by the fact that inhibition of p53 activity by pifithrin-alpha reduced the sensitivity of HCT116/p21(-/-) cells to daunomycin-induced apoptosis and restored a Bax/Bcl-2 ratio similar to that observed in HCT116p21(+/+) cells. pifithrin 120-135 tumor protein p53 Homo sapiens 104-107 12509264-1 2003 We investigated the effect of pifithrin-alpha (PFTalpha), a chemical inhibitor of p53, on DNA double-strand break (DSB) repair in mammalian chromosomes. pifithrin 30-45 tumor protein p53 Homo sapiens 82-85 12509264-1 2003 We investigated the effect of pifithrin-alpha (PFTalpha), a chemical inhibitor of p53, on DNA double-strand break (DSB) repair in mammalian chromosomes. pifithrin 47-55 tumor protein p53 Homo sapiens 82-85 12151395-5 2002 Moreover, the role of p53 in activation of this pathway was demonstrated by the fact that inhibition of p53 activity by pifithrin-alpha reduced the sensitivity of HCT116/p21(-/-) cells to daunomycin-induced apoptosis and restored a Bax/Bcl-2 ratio similar to that observed in HCT116p21(+/+) cells. pifithrin 120-135 tumor protein p53 Homo sapiens 22-25 12131363-13 2002 Pifithrin-alpha (Calbiochem), a p53 inhibitor, blocked resveratrol induced serine-15 phosphorylation of p53 and p53 binding to DNA. pifithrin 0-15 tumor protein p53 Homo sapiens 104-107 12131363-13 2002 Pifithrin-alpha (Calbiochem), a p53 inhibitor, blocked resveratrol induced serine-15 phosphorylation of p53 and p53 binding to DNA. pifithrin 0-15 tumor protein p53 Homo sapiens 104-107 11904448-6 2002 We also establish that wild-type- and mutated PS2-induced caspase activation is reduced by p53 antisense approach and by pifithrin-alpha, a chemical inhibitor of p53. pifithrin 121-136 tumor protein p53 Homo sapiens 162-165 11931974-9 2002 Addition of pifithrin-alpha (PFT), a specific inhibitor of p53, reduced the activation of p53 with a concomitant decrease in growth arrest at S phase. pifithrin 12-27 tumor protein p53 Homo sapiens 59-62 11889192-4 2002 Addition of pifithrin-alpha, a specific inhibitor of p53, or transfection of p53 antisense oligonucleotides caused decreased RV-induced p53 and p21 expression in PTC and FTC cells. pifithrin 12-27 tumor protein p53 Homo sapiens 53-56 11279278-2 2001 Based upon recent studies characterizing chemical inhibitors of p53 in preclinical studies in the cancer therapy field, we synthesized the compound pifithrin-alpha and evaluated its potential neuroprotective properties in experimental models relevant to the pathogenesis of stroke and neurodegenerative disorders. pifithrin 148-163 tumor protein p53 Homo sapiens 64-67 11827710-0 2002 Pifithrin-alpha, an inhibitor of p53, enhances the genetic instability induced by etoposide (VP16) in human lymphoblastoid cells treated in vitro. pifithrin 0-15 tumor protein p53 Homo sapiens 33-36 11931974-9 2002 Addition of pifithrin-alpha (PFT), a specific inhibitor of p53, reduced the activation of p53 with a concomitant decrease in growth arrest at S phase. pifithrin 12-27 tumor protein p53 Homo sapiens 90-93 34202551-9 2021 Treatment Vero cells with the p53 inhibitor, PFT-alpha, could significantly inhibit PEDV-induced apoptosis. pifithrin 45-54 tumor protein p53 Homo sapiens 30-33 34707773-7 2021 More importantly, STAT3 overexpression, SLC7A11 overexpression, and pretreatment with pifithrin-alpha (P53 inhibitor) rescued OS cell ferroptosis induced by bavachin. pifithrin 86-101 tumor protein p53 Homo sapiens 103-106 34469060-12 2021 Inhibition of p53 by PFT-alpha partially restored the levels of PD-L1 and miR-34a-5p. pifithrin 21-30 tumor protein p53 Homo sapiens 14-17 34427098-9 2021 Furthermore, CDKL3 knockdown increases p53 expression and reduces cell viability, and these effects are significantly weakened by the p53 inhibitor, PFT-alpha. pifithrin 149-158 tumor protein p53 Homo sapiens 39-42 34427098-9 2021 Furthermore, CDKL3 knockdown increases p53 expression and reduces cell viability, and these effects are significantly weakened by the p53 inhibitor, PFT-alpha. pifithrin 149-158 tumor protein p53 Homo sapiens 134-137 34945706-4 2021 MDA-MB-231 (p53-p.R280K mutant) and MCF-7 (wild-type p53) breast tumor cells and MCF-10A non-tumoral cells were exposed to GTE for 24-48 h and cell viability was assessed in the presence of p53 inhibitor pifithrin-alpha. pifithrin 204-219 tumor protein p53 Homo sapiens 190-193 34959709-8 2021 Moreover, treatment with the p53 inhibitor pifithrin-alpha (PFT-alpha) inhibited autophagy and increased apoptotic response in MLPE-treated HepG2 cells. pifithrin 43-58 tumor protein p53 Homo sapiens 29-32 34959709-8 2021 Moreover, treatment with the p53 inhibitor pifithrin-alpha (PFT-alpha) inhibited autophagy and increased apoptotic response in MLPE-treated HepG2 cells. pifithrin 60-69 tumor protein p53 Homo sapiens 29-32 34959709-10 2021 Thus, co-treatment with MLPE and PFT-alpha significantly increased caspase-3, caspase-8, and cytochrome c release, indicating that p53 deficiency caused the apoptosis. pifithrin 33-42 tumor protein p53 Homo sapiens 131-134 35103478-11 2022 Notably, P53 activation with Nutilin3 significantly weakened the epithelial-mesenchymal transformation (EMT) promotion effect of ASF1B, while P53 inhibition with pifithrin-alpha significantly enhanced the EMT promotion effect of sh-ASF1B. pifithrin 162-177 tumor protein p53 Homo sapiens 142-145 35530319-10 2022 Knockdown of p53 or inhibition of p53"s transcriptional activity by addition of its specific inhibitor PFT-alpha decreased expression of ALKBH5 and CSCs" malignancies, including proliferation, invasion, and tumor formation ability, indicating that p53 may partially regulate CSC"s malignancies via ALKBH5. pifithrin 103-112 tumor protein p53 Homo sapiens 34-37 35530319-10 2022 Knockdown of p53 or inhibition of p53"s transcriptional activity by addition of its specific inhibitor PFT-alpha decreased expression of ALKBH5 and CSCs" malignancies, including proliferation, invasion, and tumor formation ability, indicating that p53 may partially regulate CSC"s malignancies via ALKBH5. pifithrin 103-112 tumor protein p53 Homo sapiens 248-251 35356282-14 2022 Pre-treatment with rapamycin or PFT-alpha significantly down-regulated the levels of SA beta-Gal, SAHF, p-p53, p21, autophagy related protein p62, the percentage of cells in the G0/G1 phase, and significantly up-regulated DeltaPsim, autophagy related protein BECN1, autophagosomes and autolysosomes compared with cells only treated with AOPPs. pifithrin 32-41 tumor protein p53 Homo sapiens 106-109 35337799-7 2022 The results show that P53 mediates the dissociation induced-ferroptosis in hESCs which is suppressed by Pifithrin alpha. pifithrin 104-119 tumor protein p53 Homo sapiens 22-25 35530319-10 2022 Knockdown of p53 or inhibition of p53"s transcriptional activity by addition of its specific inhibitor PFT-alpha decreased expression of ALKBH5 and CSCs" malignancies, including proliferation, invasion, and tumor formation ability, indicating that p53 may partially regulate CSC"s malignancies via ALKBH5. pifithrin 103-112 tumor protein p53 Homo sapiens 13-16 35418961-9 2022 The treatment of PFT-alpha (p53 inhibitor) significantly suppressed the expression of cleaved caspase 9 and caspase 3, leading to the decrease of apoptosis. pifithrin 17-26 tumor protein p53 Homo sapiens 28-31 35356282-5 2022 In some experiments, cells were pre-treated with rapamycin (an activator of autophagy), 3-MA (an inhibitor of autophagy), or cyclic pifithrin-alpha (PFT-alpha, an antagonist of p53), and then treated with AOPPs. pifithrin 149-158 tumor protein p53 Homo sapiens 177-180 33510838-7 2021 Knockdown ribosomal protein L11 (RPL11) by transfecting with siRNA or inhibiting p53 by pifithrin-alpha (PFT-alpha) partly reserved the biological effects induced by BOP1 knockdown. pifithrin 88-103 tumor protein p53 Homo sapiens 81-84 34055057-8 2021 Of note, hirsutanol A induced the expression of the tumor suppressor p53, whereas simultaneous treatment with pifithrin-alpha, an inhibitor of p53, significantly reduced Jurkat cell apoptosis induced by hirsutanol A. pifithrin 110-125 tumor protein p53 Homo sapiens 143-146 33945996-8 2021 Additionally, the expression of these proteins can be reversed by the use of pifithrin-alpha (PFT-alpha), a p53 inhibitor. pifithrin 77-92 tumor protein p53 Homo sapiens 108-111 33945996-8 2021 Additionally, the expression of these proteins can be reversed by the use of pifithrin-alpha (PFT-alpha), a p53 inhibitor. pifithrin 94-103 tumor protein p53 Homo sapiens 108-111 33877544-12 2021 BOP1 silencing-induced suppression of cell proliferation was partly reversed by pifithrin-alpha (a p53 inhibitor). pifithrin 80-95 tumor protein p53 Homo sapiens 99-102 35215995-9 2022 The ROS scavenger N-acetyl-l-cysteine (NAC) and the p53 specific inhibitor Pifithrin-alpha (PFT-alpha) suppressed PEDV-induced apoptosis and impeded viral replication, suggesting that ROS and p53 play an important role in PEDV-induced apoptosis and viral replication. pifithrin 75-90 tumor protein p53 Homo sapiens 52-55 35215995-9 2022 The ROS scavenger N-acetyl-l-cysteine (NAC) and the p53 specific inhibitor Pifithrin-alpha (PFT-alpha) suppressed PEDV-induced apoptosis and impeded viral replication, suggesting that ROS and p53 play an important role in PEDV-induced apoptosis and viral replication. pifithrin 75-90 tumor protein p53 Homo sapiens 192-195 35215995-9 2022 The ROS scavenger N-acetyl-l-cysteine (NAC) and the p53 specific inhibitor Pifithrin-alpha (PFT-alpha) suppressed PEDV-induced apoptosis and impeded viral replication, suggesting that ROS and p53 play an important role in PEDV-induced apoptosis and viral replication. pifithrin 92-101 tumor protein p53 Homo sapiens 52-55 35215995-9 2022 The ROS scavenger N-acetyl-l-cysteine (NAC) and the p53 specific inhibitor Pifithrin-alpha (PFT-alpha) suppressed PEDV-induced apoptosis and impeded viral replication, suggesting that ROS and p53 play an important role in PEDV-induced apoptosis and viral replication. pifithrin 92-101 tumor protein p53 Homo sapiens 192-195 33510838-7 2021 Knockdown ribosomal protein L11 (RPL11) by transfecting with siRNA or inhibiting p53 by pifithrin-alpha (PFT-alpha) partly reserved the biological effects induced by BOP1 knockdown. pifithrin 105-114 tumor protein p53 Homo sapiens 81-84 32945383-13 2020 Pifithrin-alpha, an inhibitor of p53, partially reduced RSV-induced apoptosis and autophagy. pifithrin 0-15 tumor protein p53 Homo sapiens 33-36 31886905-5 2020 Western blot was used to measure the levels of p53 and its acetylated versions in cells treated with carboplatin and/or pifithrin-alpha. pifithrin 120-135 tumor protein p53 Homo sapiens 47-50 32901866-6 2020 Furthermore, the p53 inhibitor, Pifithrin-mu, diminished the augmentation in poly(ADP-ribose) polymerase (PARP) cleavage induced by OTS514 plus H2O2, while the Mdm2 antagonist, Nutlin 3, increased PARP cleavage. pifithrin 32-41 tumor protein p53 Homo sapiens 17-20 32978368-8 2020 Importantly, the antioxidant N-acetyl-cysteine and the p53 inhibitor pifithrin-alpha attenuated A1874-induced cell death and apoptosis in colon cancer cells. pifithrin 69-84 tumor protein p53 Homo sapiens 55-58 32898154-8 2020 Accordingly, in utero treatment of TashT embryos with the p53 inhibitor pifithrin-alpha decreased Ddx3y expression and abolished the otherwise more severe ENS defect in TashT males. pifithrin 72-87 tumor protein p53 Homo sapiens 58-61 32687844-10 2020 DpdtC treatment resulted in upregulation of p53, but, the addition of p53 inhibitor, PFT-alpha could significantly neutralize the action of DpdtC on ferritinophagy induction and EMT inhibition. pifithrin 85-94 tumor protein p53 Homo sapiens 70-73 32922194-10 2020 Inhibition of p53 by pifithrin-alpha significantly reduced the levels of PUMA, Bax, and PTEN but restored AKT phosphorylation in SiHa cells exposed to sesamin. pifithrin 21-36 tumor protein p53 Homo sapiens 14-17 32120128-3 2020 To strengthen the bioactivity of the scaffolds, pifithrin-alpha (PFTalpha), a p53 inhibitor that can reduce the repressive function of p53 in osteogenesis, was preloaded in the PLGA electrospinning solution. pifithrin 48-63 tumor protein p53 Homo sapiens 78-81 32757802-7 2021 The synergistic antitumor effect of API and CDDP was blocked by addition of the p53 inhibitor Pifithrin-alpha, and siRNA targeting the p53 gene in A549R cells. pifithrin 94-109 tumor protein p53 Homo sapiens 80-83 32120128-3 2020 To strengthen the bioactivity of the scaffolds, pifithrin-alpha (PFTalpha), a p53 inhibitor that can reduce the repressive function of p53 in osteogenesis, was preloaded in the PLGA electrospinning solution. pifithrin 48-63 tumor protein p53 Homo sapiens 135-138 32120128-3 2020 To strengthen the bioactivity of the scaffolds, pifithrin-alpha (PFTalpha), a p53 inhibitor that can reduce the repressive function of p53 in osteogenesis, was preloaded in the PLGA electrospinning solution. pifithrin 65-73 tumor protein p53 Homo sapiens 135-138 32771207-6 2020 Inhibition of p53 by treated with pifithrin-alpha (PFTalpha) causing augments of osteoclastogenesis and bone resorption, also reversed OPG-mediated inhibition of osteoclastogenesis by reducing the expression of TSC2. pifithrin 34-49 tumor protein p53 Homo sapiens 14-17 32534357-10 2020 The apoptosis induced by EPI in MKN-45 cells would be effectively inhibited with the treatment of p53 siRNA and p53 inhibitor PFT-alpha. pifithrin 126-135 tumor protein p53 Homo sapiens 112-115 31974452-0 2020 Pifithrin-alpha alters p53 post-translational modifications pattern and differentially inhibits p53 target genes. pifithrin 0-15 tumor protein p53 Homo sapiens 23-26 31974452-0 2020 Pifithrin-alpha alters p53 post-translational modifications pattern and differentially inhibits p53 target genes. pifithrin 0-15 tumor protein p53 Homo sapiens 96-99 31974452-1 2020 Pifithrin-alpha (PFT-alpha) is a small molecule which has been widely used as a specific inhibitor of p53 transcription activity. pifithrin 0-15 tumor protein p53 Homo sapiens 102-105 31974452-1 2020 Pifithrin-alpha (PFT-alpha) is a small molecule which has been widely used as a specific inhibitor of p53 transcription activity. pifithrin 17-26 tumor protein p53 Homo sapiens 102-105 31974452-3 2020 PFT-alpha has also been described to display potent p53-independent activity in cells. pifithrin 0-9 tumor protein p53 Homo sapiens 52-55 31974452-7 2020 PFT-alpha displayed a very limited effect on p53-dependent transcription upon its activation by Nutlin-3. pifithrin 0-9 tumor protein p53 Homo sapiens 45-48 31974452-8 2020 Moreover, PFT-alpha inhibitory effect on transcription was highly dependent on the nature of the p53 target gene. pifithrin 10-19 tumor protein p53 Homo sapiens 97-100 31974452-9 2020 PFT-alpha attenuated post-translational modifications of p53 without affecting total p53 protein level. pifithrin 0-9 tumor protein p53 Homo sapiens 57-60 31974452-10 2020 Finally, we found that PFT-alpha can decrease the level of intracellular reactive oxygen species through activation of an aryl hydrocarbon receptor (AHR)-Nrf2 axis in a p53-independent manner. pifithrin 23-32 tumor protein p53 Homo sapiens 169-172 31974452-11 2020 In conclusion, PFT-alpha inhibits only some aspects of p53 function, therefore it should be used with extreme caution to study p53-dependent processes. pifithrin 15-24 tumor protein p53 Homo sapiens 55-58 31974452-11 2020 In conclusion, PFT-alpha inhibits only some aspects of p53 function, therefore it should be used with extreme caution to study p53-dependent processes. pifithrin 15-24 tumor protein p53 Homo sapiens 127-130 31838664-4 2020 Our observations suggest that P53 suppression by LPS, pifithrin, or small interfering RNA increased the expression of the redox marker malondialdehyde. pifithrin 54-63 tumor protein p53 Homo sapiens 30-33 32256964-8 2020 As expected, an upregulation of p53 was observed after DpdtC insulting; however, the addition of a p53 inhibitor, PFT-alpha, could significantly attenuate the action of DpdtC on ferritinophagy induction and EMT inhibition. pifithrin 114-123 tumor protein p53 Homo sapiens 32-35 32256964-8 2020 As expected, an upregulation of p53 was observed after DpdtC insulting; however, the addition of a p53 inhibitor, PFT-alpha, could significantly attenuate the action of DpdtC on ferritinophagy induction and EMT inhibition. pifithrin 114-123 tumor protein p53 Homo sapiens 99-102 31841081-8 2020 When the p53 pathway of irradiated cells was inhibited by PFT-alpha, PFTmicro or p53 siRNA, the bystander damage to TK6 cells were clearly alleviated. pifithrin 58-67 tumor protein p53 Homo sapiens 9-12 31707831-13 2020 Torin1 treatment increased protein expression of p53, and these effects were inhibited by pifithrin-alpha. pifithrin 90-105 tumor protein p53 Homo sapiens 49-52 32771207-6 2020 Inhibition of p53 by treated with pifithrin-alpha (PFTalpha) causing augments of osteoclastogenesis and bone resorption, also reversed OPG-mediated inhibition of osteoclastogenesis by reducing the expression of TSC2. pifithrin 51-59 tumor protein p53 Homo sapiens 14-17 31601054-6 2019 Furthermore, the effects of cell cycle arrest and apoptosis in A549 cells which were induced by actinomycin V could be reversed by the pifithrin-alpha, a specific inhibitor of p53 transcriptional activity. pifithrin 135-150 tumor protein p53 Homo sapiens 176-179 32749126-6 2020 The effect of EGb761 on the p53 signaling pathway was further confirmed by adding pifithrin (PFT)-alpha, an inhibitor of p53. pifithrin 82-91 tumor protein p53 Homo sapiens 28-31 32749126-6 2020 The effect of EGb761 on the p53 signaling pathway was further confirmed by adding pifithrin (PFT)-alpha, an inhibitor of p53. pifithrin 82-91 tumor protein p53 Homo sapiens 121-124 32749126-6 2020 The effect of EGb761 on the p53 signaling pathway was further confirmed by adding pifithrin (PFT)-alpha, an inhibitor of p53. pifithrin 93-96 tumor protein p53 Homo sapiens 28-31 32749126-6 2020 The effect of EGb761 on the p53 signaling pathway was further confirmed by adding pifithrin (PFT)-alpha, an inhibitor of p53. pifithrin 93-96 tumor protein p53 Homo sapiens 121-124 30543781-7 2019 TCCP treatment caused endogenous ROS generation, increase in intracellular calcium and phosphorylation of p53 in a concentration-dependent manner, which was reverted upon pre-treatment with pifithrin-mu. pifithrin 190-199 tumor protein p53 Homo sapiens 106-109 30628118-10 2019 The cytotoxic effects of solasonine correlated in part with its apoptotic properties and differed in the two HCC cell lines, being reversed by pifithrin-alpha, an inhibitor of p53 functions, in HepG2 cells but not in Hep3b cells. pifithrin 143-158 tumor protein p53 Homo sapiens 176-179 31334116-5 2019 In addition, co-treatment with a p53 inhibitor (cyclic pifithrin-alpha) and the ruthenium complexes significantly reduced the apoptosis rate in HCT116 cells, and increased phospho-p53 (S15) and phospho-histone H2AX (S139) expressions, indicating induction of DNA damage and p53-dependent apoptosis. pifithrin 55-64 tumor protein p53 Homo sapiens 33-36 31334116-5 2019 In addition, co-treatment with a p53 inhibitor (cyclic pifithrin-alpha) and the ruthenium complexes significantly reduced the apoptosis rate in HCT116 cells, and increased phospho-p53 (S15) and phospho-histone H2AX (S139) expressions, indicating induction of DNA damage and p53-dependent apoptosis. pifithrin 55-64 tumor protein p53 Homo sapiens 180-183 31334116-5 2019 In addition, co-treatment with a p53 inhibitor (cyclic pifithrin-alpha) and the ruthenium complexes significantly reduced the apoptosis rate in HCT116 cells, and increased phospho-p53 (S15) and phospho-histone H2AX (S139) expressions, indicating induction of DNA damage and p53-dependent apoptosis. pifithrin 55-64 tumor protein p53 Homo sapiens 180-183 27025925-6 2019 Caspase-3 inhibitor (Ac-DEVD-CHO at concentrations between 10 to 20 mumol/L), p53 inhibitor (pifithrin-alpha at 5 mumol/L) and cyclosporin A can attenuate the apoptotic effect of psoralidin. pifithrin 93-108 tumor protein p53 Homo sapiens 78-81 31155771-7 2019 Additionally, under the pretreatment of pifithrin-a (PFT-a, a p53 inhibitor), the magnolol-induced apoptosis was significantly reversed. pifithrin 40-51 tumor protein p53 Homo sapiens 62-65 31155771-7 2019 Additionally, under the pretreatment of pifithrin-a (PFT-a, a p53 inhibitor), the magnolol-induced apoptosis was significantly reversed. pifithrin 53-58 tumor protein p53 Homo sapiens 62-65 30879173-6 2019 The cytotoxicity of RH1 was inhibited in A549 cells treated with the p53-inhibitor pifithrin-alpha or transfected p53 siRNA and in human colon cancer HCT116 isogenic (p53-/-) cells. pifithrin 83-98 tumor protein p53 Homo sapiens 69-72 31138778-8 2019 The p53 inhibitor pifithrin-alpha attenuated the influences of ZCCHC10 overexpression on p53 pathway, cell cycle, apoptosis, and epithelial-mesenchymal transition, whereas the p53 activator Nutlin3 could reverse the effects of ZCCHC10 knockdown. pifithrin 18-33 tumor protein p53 Homo sapiens 4-7 31138778-8 2019 The p53 inhibitor pifithrin-alpha attenuated the influences of ZCCHC10 overexpression on p53 pathway, cell cycle, apoptosis, and epithelial-mesenchymal transition, whereas the p53 activator Nutlin3 could reverse the effects of ZCCHC10 knockdown. pifithrin 18-33 tumor protein p53 Homo sapiens 89-92 31138778-8 2019 The p53 inhibitor pifithrin-alpha attenuated the influences of ZCCHC10 overexpression on p53 pathway, cell cycle, apoptosis, and epithelial-mesenchymal transition, whereas the p53 activator Nutlin3 could reverse the effects of ZCCHC10 knockdown. pifithrin 18-33 tumor protein p53 Homo sapiens 89-92 30660711-7 2019 In some assay p53 activity of NSCLC ( A549 and H460) cells were blocked with pifithrin-a, prior to treatment with ZQT. pifithrin 77-88 tumor protein p53 Homo sapiens 14-17 30472223-8 2019 Secondly, PFTalpha decreased formation of p53/mdm2 complex responsible for p53 degradation by inhibiting the protein expression of mdm2, so p53 degradation was decreased in cytoplasm and p53 accumulation was increased in nucleus, which induced more cells undergo apoptosis. pifithrin 10-18 tumor protein p53 Homo sapiens 75-78 30472223-0 2019 Pifithrin-alpha enhancing anticancer effect of topotecan on p53-expressing cancer cells. pifithrin 0-9 tumor protein p53 Homo sapiens 60-63 30472223-3 2019 In this study, pifithrin-alpha (PFTalpha), a p53 inhibitor, was used 2 h before treated with TPT on three kinds of cancer cell lines including MCF7, BGC823 and HepG2 cells. pifithrin 15-30 tumor protein p53 Homo sapiens 45-48 30472223-3 2019 In this study, pifithrin-alpha (PFTalpha), a p53 inhibitor, was used 2 h before treated with TPT on three kinds of cancer cell lines including MCF7, BGC823 and HepG2 cells. pifithrin 32-40 tumor protein p53 Homo sapiens 45-48 30472223-5 2019 It was demonstrated that PFTalpha decreases the p-p53 levels and p-p53 activity, not affects p53 expression in p53 positive tumor cells. pifithrin 25-33 tumor protein p53 Homo sapiens 50-53 30472223-5 2019 It was demonstrated that PFTalpha decreases the p-p53 levels and p-p53 activity, not affects p53 expression in p53 positive tumor cells. pifithrin 25-33 tumor protein p53 Homo sapiens 67-70 30472223-5 2019 It was demonstrated that PFTalpha decreases the p-p53 levels and p-p53 activity, not affects p53 expression in p53 positive tumor cells. pifithrin 25-33 tumor protein p53 Homo sapiens 67-70 30472223-5 2019 It was demonstrated that PFTalpha decreases the p-p53 levels and p-p53 activity, not affects p53 expression in p53 positive tumor cells. pifithrin 25-33 tumor protein p53 Homo sapiens 67-70 30472223-8 2019 Secondly, PFTalpha decreased formation of p53/mdm2 complex responsible for p53 degradation by inhibiting the protein expression of mdm2, so p53 degradation was decreased in cytoplasm and p53 accumulation was increased in nucleus, which induced more cells undergo apoptosis. pifithrin 10-18 tumor protein p53 Homo sapiens 42-45 30472223-8 2019 Secondly, PFTalpha decreased formation of p53/mdm2 complex responsible for p53 degradation by inhibiting the protein expression of mdm2, so p53 degradation was decreased in cytoplasm and p53 accumulation was increased in nucleus, which induced more cells undergo apoptosis. pifithrin 10-18 tumor protein p53 Homo sapiens 75-78 30551455-8 2019 Suppressing p53 expressions with its inhibitor, PFTalpha, blocked apoptotic response in JMJD5-silenced cells. pifithrin 48-56 tumor protein p53 Homo sapiens 12-15 30145226-6 2019 Treatment with pifithrin-alpha-HBr (PFTalpha), a specific blocker of p53-responsive gene transactivation, reduced the P4-increased p27 promoter activity and p27 protein expression. pifithrin 36-44 tumor protein p53 Homo sapiens 69-72 30472223-8 2019 Secondly, PFTalpha decreased formation of p53/mdm2 complex responsible for p53 degradation by inhibiting the protein expression of mdm2, so p53 degradation was decreased in cytoplasm and p53 accumulation was increased in nucleus, which induced more cells undergo apoptosis. pifithrin 10-18 tumor protein p53 Homo sapiens 75-78 30545932-8 2018 Correspondingly, embryos cultured with p53 transcriptional activity inhibitor pifithrin-alpha could overcome TPEN-induced apoptosis and failure of neural tube closure. pifithrin 78-87 tumor protein p53 Homo sapiens 39-42 30103008-11 2018 Both p53 inhibitor pifithrin-alpha and p53 siRNA significantly rescued costunolide suppression of GLS1 promoter activity and expression in HCT116 cells. pifithrin 19-34 tumor protein p53 Homo sapiens 5-8 30080692-8 2018 We further found that pifithrin-alpha, a p53 inhibitor, attenuated the anticancer effects of VX-680 and downregulated the expression of apotosis-related proteins (Bax and caspase-9). pifithrin 22-37 tumor protein p53 Homo sapiens 41-44 30272369-5 2018 Subsequently, the expression trends of Bcl-2 and p53 were reversed after co-treatment with pifithrin-alpha (PFT-alpha, a p53 inhibitor). pifithrin 91-106 tumor protein p53 Homo sapiens 49-52 30272369-5 2018 Subsequently, the expression trends of Bcl-2 and p53 were reversed after co-treatment with pifithrin-alpha (PFT-alpha, a p53 inhibitor). pifithrin 91-106 tumor protein p53 Homo sapiens 121-124 29802837-6 2018 The dissociation-induced increase of phosphorylated p53 Ser15 (p-p53) is suppressed by Pifithrin alpha which also rescues the elevated levels of pro-apoptotic proteins in mitochondrial pathway. pifithrin 87-102 tumor protein p53 Homo sapiens 52-55 29944886-12 2018 Moreover, suppressing p53 expression with its inhibitor of PFTalpha abrogated the activity of LHPP to impede cell proliferation and metastasis, and to trigger apoptosis. pifithrin 59-67 tumor protein p53 Homo sapiens 22-25 29802837-6 2018 The dissociation-induced increase of phosphorylated p53 Ser15 (p-p53) is suppressed by Pifithrin alpha which also rescues the elevated levels of pro-apoptotic proteins in mitochondrial pathway. pifithrin 87-102 tumor protein p53 Homo sapiens 65-68 29525471-6 2018 Prolonged expression of p53 was responsible for melanocyte senescence and hyperpigmentation, and treatment with the p53-inhibitor pifithrin-alpha at 2-weeks post-UVB irradiation, but not at 48 h, significantly reduced melanin content along with decreases in tyrosinase levels. pifithrin 130-145 tumor protein p53 Homo sapiens 24-27 29525471-6 2018 Prolonged expression of p53 was responsible for melanocyte senescence and hyperpigmentation, and treatment with the p53-inhibitor pifithrin-alpha at 2-weeks post-UVB irradiation, but not at 48 h, significantly reduced melanin content along with decreases in tyrosinase levels. pifithrin 130-145 tumor protein p53 Homo sapiens 116-119 29207130-10 2018 Furthermore, treatment or co-treatment with LY294002 (phosphoinositide-3-kinase/Akt inhibitor) or Pifithrin-alpha (p53 inhibitor) with CME resulted in CME-induced G1 arrest which occurred through the p53-independent signaling pathway in hepatocellular carcinoma cells. pifithrin 98-113 tumor protein p53 Homo sapiens 115-118 31938352-11 2018 The protein levels of bax and cl-caspase-3 were significantly decreased and the expression of bcl-2 was increased after pretreated with p53 specific inhibitor pifithrin-alpha. pifithrin 159-174 tumor protein p53 Homo sapiens 136-139 29512370-4 2018 For this purpose, mixed lymphocyte reaction (MLR) was performed using the IDO inhibitor 1-DL-methyl-tryptophan and the p53 inhibitor pifithrin-alpha. pifithrin 133-148 tumor protein p53 Homo sapiens 119-122 29467390-14 2018 Indeed, p53 accumulates at mitochondria upon etoposide treatment and inhibition of p53 mitochondrial localization using pifithrin-micro inhibits apoptosis of COV434 cells. pifithrin 120-129 tumor protein p53 Homo sapiens 83-86 29207130-10 2018 Furthermore, treatment or co-treatment with LY294002 (phosphoinositide-3-kinase/Akt inhibitor) or Pifithrin-alpha (p53 inhibitor) with CME resulted in CME-induced G1 arrest which occurred through the p53-independent signaling pathway in hepatocellular carcinoma cells. pifithrin 98-113 tumor protein p53 Homo sapiens 200-203 29278895-5 2018 RESULTS: In cultures of myogenic cells from MDC1A donors, p53 accumulated in a subset of nuclei and aberrant caspase activation was inhibited by the p53 inhibitor pifithrin-alpha. pifithrin 163-178 tumor protein p53 Homo sapiens 58-61 29278895-5 2018 RESULTS: In cultures of myogenic cells from MDC1A donors, p53 accumulated in a subset of nuclei and aberrant caspase activation was inhibited by the p53 inhibitor pifithrin-alpha. pifithrin 163-178 tumor protein p53 Homo sapiens 149-152 29212215-5 2017 We examined the molecular mechanism of this cell death pathway, and found that B2 protein induces the P53/Bax-mediated apoptotic pathway in A549 cells, and that a P53 specific inhibitor (pifithrin-alpha) switches this response to RIP3-mediated necroptosis. pifithrin 187-196 tumor protein p53 Homo sapiens 102-105 28990231-8 2017 Mechanistically, IFI16 could activate p53 at Ser15 to up-regulate the p21WAF1/CIP1 level to inhibit tumour growth and migration, which was restored by the p53 inhibitor Pifithrin-alpha (20 mumol/L). pifithrin 169-184 tumor protein p53 Homo sapiens 38-41 28990231-8 2017 Mechanistically, IFI16 could activate p53 at Ser15 to up-regulate the p21WAF1/CIP1 level to inhibit tumour growth and migration, which was restored by the p53 inhibitor Pifithrin-alpha (20 mumol/L). pifithrin 169-184 tumor protein p53 Homo sapiens 155-158 29039478-7 2017 In particular, inhibition of p53 by pifithrin-alpha, and upregulation of eIF2alpha phosphorylation by Sal003, reduced cisplatin-induced apoptosis. pifithrin 36-45 tumor protein p53 Homo sapiens 29-32 28691888-10 2017 The p53 inhibitor, Pifithrin-alpha, blocked the gingipain-induced Bid. pifithrin 19-34 tumor protein p53 Homo sapiens 4-7 29212215-5 2017 We examined the molecular mechanism of this cell death pathway, and found that B2 protein induces the P53/Bax-mediated apoptotic pathway in A549 cells, and that a P53 specific inhibitor (pifithrin-alpha) switches this response to RIP3-mediated necroptosis. pifithrin 187-196 tumor protein p53 Homo sapiens 163-166 32719725-5 2017 Further study showed that TFS decreased mitochondrial membrane potential, activated Caspase-3/7, Caspase-8 and Caspase-9 activities, and that the p53 inhibitor PFT-alpha reversed the TFS-induced cell growth inhibition and apoptosis. pifithrin 160-169 tumor protein p53 Homo sapiens 146-149 28098861-10 2017 Treatment of HepG2 cells with p53 inhibitor, pifithrin-alpha prior to incubation with triptolide significantly prevented induction of cell apoptosis. pifithrin 45-60 tumor protein p53 Homo sapiens 30-33 28431961-7 2017 Senescence was significantly inhibited by treatment with pifithrin-alpha, a p53 inhibitor, or by MnTBAP, a superoxide dismutase mimetic, validating those molecular actors in IR-induced endothelial cell aging. pifithrin 57-66 tumor protein p53 Homo sapiens 76-79 28631571-14 2017 The above-mentioned characteristics were reverted by treatment of with pifithrin-a, a p53 inhibitor. pifithrin 71-82 tumor protein p53 Homo sapiens 86-89 28028695-9 2017 An increase in p53 levels was detected following 5-FU treatment; pifithrin-alpha, an inhibitor of p53 activation, reversed 5-FU-induced SESN2 expression. pifithrin 65-74 tumor protein p53 Homo sapiens 15-18 28028695-9 2017 An increase in p53 levels was detected following 5-FU treatment; pifithrin-alpha, an inhibitor of p53 activation, reversed 5-FU-induced SESN2 expression. pifithrin 65-74 tumor protein p53 Homo sapiens 98-101 28143426-9 2017 The impact of functional p53 was investigated by siRNA gene silencing and the p53 inhibitor pifithrin-alpha. pifithrin 92-107 tumor protein p53 Homo sapiens 25-28 28143426-9 2017 The impact of functional p53 was investigated by siRNA gene silencing and the p53 inhibitor pifithrin-alpha. pifithrin 92-107 tumor protein p53 Homo sapiens 78-81 27555286-6 2016 Specific inhibition of p53 by pifithrin-alpha reversed the G2/M phase arrest induced by the 2b compound, suggesting contribution of p53 to increase. pifithrin 30-39 tumor protein p53 Homo sapiens 23-26 28035355-9 2017 Additionally, suppression of p53 using a specific inhibitor, pifithrin-alpha, abrogated the pro-apoptotic effects of Scutellarin in HCT-116 cells. pifithrin 61-76 tumor protein p53 Homo sapiens 29-32 28321407-7 2017 When using Pifithrin-alpha (one of p53 inhibitors), the level of p53-dependent apoptosis was significantly lower than untreated, which suggested that p53 was possibly involved in PTEN-dependent apoptosis. pifithrin 11-26 tumor protein p53 Homo sapiens 35-38 28321407-7 2017 When using Pifithrin-alpha (one of p53 inhibitors), the level of p53-dependent apoptosis was significantly lower than untreated, which suggested that p53 was possibly involved in PTEN-dependent apoptosis. pifithrin 11-26 tumor protein p53 Homo sapiens 65-68 28321407-7 2017 When using Pifithrin-alpha (one of p53 inhibitors), the level of p53-dependent apoptosis was significantly lower than untreated, which suggested that p53 was possibly involved in PTEN-dependent apoptosis. pifithrin 11-26 tumor protein p53 Homo sapiens 65-68 27471307-9 2016 P53 and p21 expression were upregulated in MSC-ITP, but inhibition of p53 with pifithrin-alpha markedly inhibited apoptosis and senescence. pifithrin 79-94 tumor protein p53 Homo sapiens 70-73 27470413-10 2016 Moreover, the inhibiting effects and apoptotic related proteins responses to Ch-1 on Hela cells were abolished after pre-treated with Pifithrin-alpha (Pft-alpha), a p53 inhibitor. pifithrin 134-149 tumor protein p53 Homo sapiens 165-168 27591796-8 2016 Increased Nrf2 expression and catalase activity at 40 C were inhibited by the antioxidant PEG-catalase and by p53 inhibitor pifithrin-alpha. pifithrin 124-139 tumor protein p53 Homo sapiens 110-113 27327234-6 2016 Pifithrin-alpha (PFT), an inhibitor of p53, significantly enhanced ARS-induced cytotoxicity in HepG2 cells, and the forced expression of wild-type p53 significantly enhanced ARS-induced cytotoxicity in Hep3B cells. pifithrin 0-15 tumor protein p53 Homo sapiens 39-42 27327234-6 2016 Pifithrin-alpha (PFT), an inhibitor of p53, significantly enhanced ARS-induced cytotoxicity in HepG2 cells, and the forced expression of wild-type p53 significantly enhanced ARS-induced cytotoxicity in Hep3B cells. pifithrin 17-20 tumor protein p53 Homo sapiens 39-42 27402273-12 2016 Pifithrin-alpha, a p53 inhibitor, slightly inhibited growth of A549 cells and under p53 inhibitory condition evodiamine-induced apoptosis could not be reversed. pifithrin 0-15 tumor protein p53 Homo sapiens 19-22 27402273-12 2016 Pifithrin-alpha, a p53 inhibitor, slightly inhibited growth of A549 cells and under p53 inhibitory condition evodiamine-induced apoptosis could not be reversed. pifithrin 0-15 tumor protein p53 Homo sapiens 84-87 26636375-3 2016 In the present study by using the specific inhibitors Ku55933 and Pifithrin-alpha, we confirmed implication of both ATM and p53 in H(2)O(2)-induced senescence of hMESCs. pifithrin 66-75 tumor protein p53 Homo sapiens 124-127 27064011-5 2016 Pifithrin-alpha (PFN-alpha), a p53 inhibitor, was pretreated into the cells. pifithrin 0-15 tumor protein p53 Homo sapiens 31-34 27270209-8 2016 Inhibition of p53 by pifithrin-alpha, attenuates citral-mediated apoptosis. pifithrin 21-36 tumor protein p53 Homo sapiens 14-17 26250568-5 2015 When cells were pretreated with a p53 inhibitor (pifithrin-a), followed by magnolol treatment, pifithrin-a blocked magnolol-induced apoptosis and G0 /G1 arrest. pifithrin 95-106 tumor protein p53 Homo sapiens 34-37 26434996-6 2015 The energy metabolism phenotype of HeLa-H cells was reverted to that of HeLa-L cells by incubating with pifithrin-alpha, a p53-inhibitor. pifithrin 104-119 tumor protein p53 Homo sapiens 123-126 26250568-5 2015 When cells were pretreated with a p53 inhibitor (pifithrin-a), followed by magnolol treatment, pifithrin-a blocked magnolol-induced apoptosis and G0 /G1 arrest. pifithrin 49-60 tumor protein p53 Homo sapiens 34-37 26703711-4 2015 The application of pifithrin mu as an inhibitor of p53 shuttling to mitochondria reduced RV-induced cell death to an extent similar to that of the broad spectrum caspase inhibitor z-VAD-fmk (benzyloxycarbonyl-V-A-D-(OMe)-fmk). pifithrin 19-28 tumor protein p53 Homo sapiens 51-54 26302161-6 2015 Inhibition of p53 activity using either pifithrin-alpha or small interference RNA interference reduced OGD-induced cell death and pifithrin-alpha reversed OGD-induced impairment of glutamate uptake in astrocytes, suggesting that p53 might play a key role in mediating neurotoxicity and gliotoxicity in ischemic brain injury. pifithrin 40-49 tumor protein p53 Homo sapiens 14-17 26302161-6 2015 Inhibition of p53 activity using either pifithrin-alpha or small interference RNA interference reduced OGD-induced cell death and pifithrin-alpha reversed OGD-induced impairment of glutamate uptake in astrocytes, suggesting that p53 might play a key role in mediating neurotoxicity and gliotoxicity in ischemic brain injury. pifithrin 130-139 tumor protein p53 Homo sapiens 14-17 26170659-13 2015 Cells pretreated with pifithrin-alpha were protected from p53-mediated AgNPs-induced toxicity. pifithrin 22-37 tumor protein p53 Homo sapiens 58-61 25575314-3 2015 While pifithrin-alpha, a specific p53 inhibitor, induced cell cycle arrest in G1 phase. pifithrin 6-21 tumor protein p53 Homo sapiens 34-37 25955698-8 2015 Pre-treatment with the p53 inhibitor, pifithrin-alpha, prevented apoptosis and the changes in IGF-1R and IGFBP-3 elicited by doxorubicin. pifithrin 38-47 tumor protein p53 Homo sapiens 23-26 25938491-9 2015 Specific inhibition of p53 by pifithrin-alpha reduces the percentage of cells accumulated in S-phase, suggesting contribution of p53 to S-phase increase. pifithrin 30-45 tumor protein p53 Homo sapiens 23-26 25793965-5 2015 The 1-induced degradation of beta-catenin was also abrogated in the presence of pifithrin-alpha, an inhibitor of p53 transcriptional activity. pifithrin 80-95 tumor protein p53 Homo sapiens 113-116 25713322-6 2015 A similar effect was induced by the p53 inhibitor pifithrin, which also potentiated the LPS-induced hyperpermeability in human lung microvascular endothelial cells (HLMVEC). pifithrin 50-59 tumor protein p53 Homo sapiens 36-39 25575314-4 2015 Although 1, 2 and pifithrin-alpha caused serious inhibition on p53, 1 and 2 significantly cause the loss of mitochondrial membrane potential and increase of the reactive oxygen species level, cytochrome c, apaf-1 and caspase-3/9 ratio in BEL-7404 cells. pifithrin 18-27 tumor protein p53 Homo sapiens 63-75 25435953-15 2015 Subsequent to the use of Pifithrin-alpha, a specific inhibitor of p53 activation, increased MDM2 expression was observed in the LIRR1-overexpressing cells, suggesting that LIRR1 could mediate the DNA damage response (DDR) signaling in a p53-dependent manner. pifithrin 25-40 tumor protein p53 Homo sapiens 66-69 25641429-5 2015 Furthermore, the enhancement of p53-dependent apoptosis by flavokawain B could be rescued by pifithrin-alpha, a pharmacological inhibitor of p53 transcriptional activity. pifithrin 93-108 tumor protein p53 Homo sapiens 32-35 25641429-5 2015 Furthermore, the enhancement of p53-dependent apoptosis by flavokawain B could be rescued by pifithrin-alpha, a pharmacological inhibitor of p53 transcriptional activity. pifithrin 93-108 tumor protein p53 Homo sapiens 141-144 25658320-7 2015 To a different extent, either the antioxidant N-acetyl-cysteine or the p53 inhibitor, Pifithrin-alpha, recover cell viability and decrease ROS formation. pifithrin 86-101 tumor protein p53 Homo sapiens 71-74 25544361-8 2015 These effects were blocked by pretreatment with pifithrin-alpha, a p53 inhibitor. pifithrin 48-63 tumor protein p53 Homo sapiens 67-70 25737737-6 2015 Treatment of cells with pifithrin-alpha, a specific inhibitor of p53, suppressed shikonin-induced apoptosis and premature senescence, suggesting the role of p53 in mediating the actions of shikonin on regulation of lung cancer cell proliferation. pifithrin 24-39 tumor protein p53 Homo sapiens 65-68 25737737-6 2015 Treatment of cells with pifithrin-alpha, a specific inhibitor of p53, suppressed shikonin-induced apoptosis and premature senescence, suggesting the role of p53 in mediating the actions of shikonin on regulation of lung cancer cell proliferation. pifithrin 24-39 tumor protein p53 Homo sapiens 157-160 25435953-15 2015 Subsequent to the use of Pifithrin-alpha, a specific inhibitor of p53 activation, increased MDM2 expression was observed in the LIRR1-overexpressing cells, suggesting that LIRR1 could mediate the DNA damage response (DDR) signaling in a p53-dependent manner. pifithrin 25-40 tumor protein p53 Homo sapiens 237-240 25019212-4 2014 This indicates p53-independent apoptotic pathway, because response of both p53 mutant and wild type cell line were found unaffected after treatment with pifithrin-alpha, an inhibitor of p53. pifithrin 153-168 tumor protein p53 Homo sapiens 15-18 25495224-3 2014 RESULTS: The effect of IL-1beta, but not of TNF-alpha, on glutamate-mediated excitatory postsynaptic currents was blocked by pifithrin-alpha (PFT), inhibitor of p53. pifithrin 125-140 tumor protein p53 Homo sapiens 161-164 25565771-9 2015 Pifithrin-alpha, a p53 inhibitor, blocked the modulation of As4S4 on AGS cells, but not on MGC803 cells. pifithrin 0-15 tumor protein p53 Homo sapiens 19-22 26579413-5 2014 The combination also decreased the MMP, down-regulated Bcl-2 and pro-proteins of the caspase family, and up-regulated Bax and BID, all of which were reversed by the p53 inhibitor, pifithrin-alpha. pifithrin 180-189 tumor protein p53 Homo sapiens 165-168 25158131-3 2014 Pifithrin-alpha, an inhibitor of p53 function, and knockdown of p53 decreased the level of frataxin mRNA in human kidney HEK 293T cells. pifithrin 0-15 tumor protein p53 Homo sapiens 33-36 25085902-6 2014 Enhanced cytotoxicities after treatment with the p53-inhibitory drug Pifithrinalpha further supported p53-mediated resistance to PARP inhibition. pifithrin 69-83 tumor protein p53 Homo sapiens 49-52 24726874-15 2014 Down-regulated the expression of tumor suppressor p53 by 10 muM pifithrin-alpha (PFTalpha) alone had no effect on adipose accumulation. pifithrin 64-79 tumor protein p53 Homo sapiens 50-53 25085902-6 2014 Enhanced cytotoxicities after treatment with the p53-inhibitory drug Pifithrinalpha further supported p53-mediated resistance to PARP inhibition. pifithrin 69-83 tumor protein p53 Homo sapiens 102-105 25205311-10 2014 Pharmacological inhibition of mitochondrial p53 using pifithrin-mu abolished the detrimental effects of SI and reduced cell death. pifithrin 54-63 tumor protein p53 Homo sapiens 44-47 24726874-15 2014 Down-regulated the expression of tumor suppressor p53 by 10 muM pifithrin-alpha (PFTalpha) alone had no effect on adipose accumulation. pifithrin 81-89 tumor protein p53 Homo sapiens 50-53 25118938-5 2014 Inhibition of p53 activity with pifithrin-alpha or inhibition of PI3K with LY294002 suppressed CdCl2-induced cellular damage and elevation of Notch1-NICD. pifithrin 32-41 tumor protein p53 Homo sapiens 14-17 24952138-8 2014 The p53 inhibitor pifithrin-alpha and the antioxidants N-acetylcysteine and glutathione (GSH) protected the cells from PEITC-mediated apoptosis. pifithrin 18-33 tumor protein p53 Homo sapiens 4-7 24343341-6 2014 The p53/Cyp-D mitochondrial complexation was prevented by CsA or Cyp-D silencing, or by p53 inhibitor pifithrin-alpha. pifithrin 102-111 tumor protein p53 Homo sapiens 4-7 24905957-7 2014 VIP effects could be blocked by cell incubation with a specific p53 inhibitor, cyclin pifithrin-alpha hydrobromide (CPFT-alphaH). pifithrin 86-114 tumor protein p53 Homo sapiens 64-67 24946211-9 2014 The anti-oxidant N-acetylcysteine (NAC), the P53 inhibitor pifithrin-alpha (PFTalpha) as well as P53 siRNA knockdown suppressed berberine-induced P53 mitochondrial translocation and Cyp-D association, thus inhibiting mitochondrial membrane potential (MMP) decrease and prostate cancer cell necrosis. pifithrin 59-74 tumor protein p53 Homo sapiens 45-48 24789349-9 2014 Furthermore, p53 inhibition using pifithrin-alpha abolished the induction of caspase-3 and PARP by FANCF shRNA and MMC, indicating that MMC-induced apoptosis is substantially enhanced by FANCF shRNA via p53-dependent mechanisms. pifithrin 34-43 tumor protein p53 Homo sapiens 13-16 24789349-9 2014 Furthermore, p53 inhibition using pifithrin-alpha abolished the induction of caspase-3 and PARP by FANCF shRNA and MMC, indicating that MMC-induced apoptosis is substantially enhanced by FANCF shRNA via p53-dependent mechanisms. pifithrin 34-43 tumor protein p53 Homo sapiens 203-206 24841907-6 2014 Moreover, the p53 inhibitor pifithrin-alpha and the nuclear factor (NF)-kappaB inhibitor pyrrolidinedithiocarbamic acid (PDTC) clearly suppressed Beclin-1 and LC3-II and increased cytochrome c release, caspase-3 activation, and PARP cleavage. pifithrin 28-37 tumor protein p53 Homo sapiens 14-17 24646317-0 2014 p53 functional inhibitors behaving like pifithrin-beta counteract the Alzheimer peptide non-beta-amyloid component effects in human SH-SY5Y cells. pifithrin 40-49 tumor protein p53 Homo sapiens 0-3 24743574-12 2014 Moreover, Bcl-2 expression was completely reversed by treatment with pifithrin-alpha, a p53-specific inhibitor. pifithrin 69-84 tumor protein p53 Homo sapiens 88-91 24343341-6 2014 The p53/Cyp-D mitochondrial complexation was prevented by CsA or Cyp-D silencing, or by p53 inhibitor pifithrin-alpha. pifithrin 102-111 tumor protein p53 Homo sapiens 88-91 26629941-6 2014 Notably, these expression changes could be neutralized by PFTalpha, an inhibitor of p53 signaling pathway that could inhibit p53-dependent transactivation of p53-responsive genes. pifithrin 58-66 tumor protein p53 Homo sapiens 84-87 24667845-5 2014 Using pifithrin-alpha(PFT), a p53"s mitochondrial translocation inhibitor, we found that pretreated with PFT, heat stress induced mitochondrial p53 translocation was significantly suppressed, accompanied by a significant alleviation in the loss of DeltaPsim, cytochrome c release and caspase-9 activation. pifithrin 6-21 tumor protein p53 Homo sapiens 30-33 24667845-5 2014 Using pifithrin-alpha(PFT), a p53"s mitochondrial translocation inhibitor, we found that pretreated with PFT, heat stress induced mitochondrial p53 translocation was significantly suppressed, accompanied by a significant alleviation in the loss of DeltaPsim, cytochrome c release and caspase-9 activation. pifithrin 6-21 tumor protein p53 Homo sapiens 144-147 25379568-10 2014 Inhibiting p53 function with pifithrin alpha prevented the promotion of HRS by VorinostatSAHA. pifithrin 29-44 tumor protein p53 Homo sapiens 11-14 24120424-8 2014 OA-induced changes in cell viability and ATP production were rescued to control levels when cells were pretreated with pifithrin-alpha (PTA), a p53 inhibitor. pifithrin 119-134 tumor protein p53 Homo sapiens 144-147 26629941-6 2014 Notably, these expression changes could be neutralized by PFTalpha, an inhibitor of p53 signaling pathway that could inhibit p53-dependent transactivation of p53-responsive genes. pifithrin 58-66 tumor protein p53 Homo sapiens 125-128 26629941-6 2014 Notably, these expression changes could be neutralized by PFTalpha, an inhibitor of p53 signaling pathway that could inhibit p53-dependent transactivation of p53-responsive genes. pifithrin 58-66 tumor protein p53 Homo sapiens 125-128