PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 14522823-2 2004 Mucin release has been measured as [3H]glucosamine-labeled product in extracellular medium and as single-cell membrane capacitance increases indicative of exocytosis-related increases in membrane area. Glucosamine 39-50 LOC100508689 Homo sapiens 0-5 15985706-3 2005 Using [(3)H]glucosamine-labeled gastric mucosal cells, we show that stimulatory effect of beta-adrenergic agonist, isoproterenol, on mucin secretion was inhibited by EGFR kinase inhibitor, PD153035, as well as wortmannin, a specific inhibitor of PI3K. Glucosamine 12-23 LOC100508689 Homo sapiens 133-138 15527548-3 2004 Using [(3)H]glucosamine-labeled mucous acinar cells of sublingual salivary gland in culture, we show that stimulatory effect of beta-adrenergic agonist, isoproterenol, on mucin secretion was inhibited in a concentration-dependent manner by EGFR kinase inhibitor, PD153035, as well as wortmannin, an inhibitor of PI3K. Glucosamine 12-23 LOC100508689 Homo sapiens 171-176 9872504-4 1998 Biosynthesis of mucin was studied by labeling gastric mucosa for 2 h with [3H]-glucosamine. Glucosamine 79-90 LOC100508689 Homo sapiens 16-21 9922317-6 1999 This reduction was associated with a 50% reduction in synthesis of mature glucosamine-labeled mucin, almost complete inhibition of secretion of sialyl-LeX and sialyl-Tn antigens, and a 40% decrease in binding of colon cancer cells to E-selectin. Glucosamine 74-85 LOC100508689 Homo sapiens 94-99 11754731-7 2002 Carbohydrate analyses of the purified mucin showed the presence of galactose, glucosamine, galactosamine, and sialic acid, but no mannose, glucose, or uronic acid. Glucosamine 78-89 LOC100508689 Homo sapiens 38-43 11425799-11 2001 The reduced sulfate:glucosamine ratio was largely due to increased incorporation of glucosamine into newly synthesized mucin rather than reduction in total sulfate incorporation. Glucosamine 20-31 LOC100508689 Homo sapiens 119-124 11425799-11 2001 The reduced sulfate:glucosamine ratio was largely due to increased incorporation of glucosamine into newly synthesized mucin rather than reduction in total sulfate incorporation. Glucosamine 84-95 LOC100508689 Homo sapiens 119-124 7515824-4 1994 We examined the effects of interferon-gamma 1) on mucin exocytosis, measured as release of [3H]glucosamine-labeled macromolecules trapped at the stacking/running gel interface of polyacrylamide gels, and 2) on mucin biosynthesis, examined at the RNA level using a cDNA probe directed to the MUC2 mucin gene. Glucosamine 95-106 LOC100508689 Homo sapiens 50-55 8443822-3 1993 Mucin was quantitated by [3H]glucosamine labeling and Sepharose CL-4B chromatography. Glucosamine 29-40 LOC100508689 Homo sapiens 0-5 8443822-10 1993 Ion-exchange (DEAE-Sephacel) chromatography of [3H]glucosamine-labeled HMG demonstrated that the acidic or basic nature of the mucin was different in all cancer cell lines tested. Glucosamine 51-62 LOC100508689 Homo sapiens 127-132 1511431-2 1992 Mucin was quantitated by [3H]glucosamine labeling and chromatography on Sepharose CL-4B. Glucosamine 29-40 LOC100508689 Homo sapiens 0-5 2775237-1 1989 Mucin from xenografts of LS174T human colon cancer cells was treated with anhydrous HF for 1 h at 0 degree C to give a product (HFA) with over 80% of the glucosamine and hexose removed, but retaining some galactosamine, and for 3 h at room temperature to give a product (HFB) devoid of carbohydrate. Glucosamine 154-165 LOC100508689 Homo sapiens 0-5 3664476-9 1987 Metabolic labeling using [3H]glucosamine demonstrated that high mucin variants synthesized 2-fold more mucin in the cell layer and secreted 3-fold more mucin into the culture medium than the low mucin variant. Glucosamine 29-40 LOC100508689 Homo sapiens 64-69 3967040-2 1985 The mucin contained 11% carbohydrate, largely as glucosamine, galactose and N-acetylneuraminic acid, and 19% lipid, of which 86% was unesterified fatty acid. Glucosamine 49-60 LOC100508689 Homo sapiens 4-9 8741012-2 1996 Mucin secretion was followed by measuring the release of [3H]-glucosamine metabolically labelled glycoproteins eluted in the void volume of Sepharose 4B column chromatography. Glucosamine 62-73 LOC100508689 Homo sapiens 0-5 8729006-4 1996 Mucin was measured by [3H]-glucosamine labeling. Glucosamine 27-38 LOC100508689 Homo sapiens 0-5 8006952-2 1994 Mucin secretion was measured as release of glucosamine-labeled macromolecules trapped at the stacking/running gel interface of polyacrylamide gels and electrolyte secretion as short-circuit current (Isc). Glucosamine 43-54 LOC100508689 Homo sapiens 0-5 1383042-7 1992 At low sulfate content in the medium and in the presence of chlorate, the incorporation of sulfate and glucosamine was mainly into the low molecular-weight form of mucin. Glucosamine 103-114 LOC100508689 Homo sapiens 164-169