PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 12782181-3 2003 Exposure of A498 cells to a cytokine mixture consisting of interferon gamma, interleukin-1 beta and tumor necrosis factor-alpha (TNF-alpha) increased nitrite production, iNOS mRNA and protein expression. Nitrites 150-157 interferon gamma Homo sapiens 59-75 27307103-7 2016 In all cell models, interferon-gamma activation leads to up-regulation of interleukin-12 and nitrite production. Nitrites 93-100 interferon gamma Homo sapiens 20-36 26500455-8 2015 Treatment with a combination of IL-6, LPS, TNF-alpha, IFN- gamma and IL-1beta induced the highest nitrite secretion (2.91 fold, P < 0.01) as compared to cells incubated in medium alone. Nitrites 98-105 interferon gamma Homo sapiens 54-64 24518246-4 2014 We found that IL-13 and IFN-gamma synergistically enhanced iNOS, nitrite, and 3NT, corresponding with increased H(2)O(2). Nitrites 65-72 interferon gamma Homo sapiens 24-33 16914097-7 2006 Our in vitro results indicate a correlation between nitrite and IFN-gamma production in PBMC culture supernatants. Nitrites 52-59 interferon gamma Homo sapiens 64-73 24024215-5 2013 It was reported previously that interferon gamma (IFN-gamma) and nitric oxide synthase 2 (NOS2) are expressed on alveolar macrophages from TB patients and are responsible for bacilli control; thus, we aimed this study at genotyping single nucleotide polymorphisms IFNG+874T/A SNP and NOS2A-954G/C SNP to estimate their role on TB susceptibility and determine whether these polymorphisms influence serum nitrite and NOx(-) production. Nitrites 403-410 interferon gamma Homo sapiens 32-59 15734263-11 2005 Exposure to lipopolysaccharide (LPS) and IFN-gamma significant increased endogenous nitrite production. Nitrites 84-91 interferon gamma Homo sapiens 41-50 15374815-7 2005 Nitrite production by HT-29 cells was significantly increased (P < 0.01) in cocultures with MMCs stimulated with IFN-gamma and LPS. Nitrites 0-7 interferon gamma Homo sapiens 116-125 15374815-9 2005 Interestingly, stimulation of HT-29 with conditioned media from MMCs pretreated with steroids before stimulation with LPS and IFN-gamma induced a significantly (P < 0.01) lower nitrite production. Nitrites 180-187 interferon gamma Homo sapiens 126-135 15115662-6 2004 Overexpression of c-Jun in hepatocytes inhibited IL-1beta+IFNgamma-induced nitrite accumulation and iNOS promoter activity while dominant negative c-Jun partially reversed the inhibitory effects of cAMP on nitrite accumulation. Nitrites 75-82 interferon gamma Homo sapiens 58-66 15115662-6 2004 Overexpression of c-Jun in hepatocytes inhibited IL-1beta+IFNgamma-induced nitrite accumulation and iNOS promoter activity while dominant negative c-Jun partially reversed the inhibitory effects of cAMP on nitrite accumulation. Nitrites 206-213 interferon gamma Homo sapiens 58-66 12202921-5 2002 In 1/3 to 2/3 of the subjects tested, sodium nitrate or nitrite decreased production of Th1 cytokines (interleukin-2, interferon-gamma, and tumor necrosis factor-beta). Nitrites 56-63 interferon gamma Homo sapiens 118-166 12620644-6 2003 When cells were co-treated with increasing doses of rhGGF2 and PMA or IFNgamma, only concentrations of 50 ng/ml, but not 10 or 100 ng/ml, were able to decrease oxidative burst activity and nitrite release. Nitrites 189-196 interferon gamma Homo sapiens 70-78 12066846-7 2002 Nitrostyrene treatment of macrophages, stimulated with IFN gamma and LPS, resulted in a dose dependent differential inhibition in IL12, IL6 and nitrite production, even using doses < 0.5 microg/mL. Nitrites 144-151 interferon gamma Homo sapiens 55-64 12351936-11 2002 Induction of nitrite production by a cytomix [IFNgamma (100 ng/ml) + TNFalpha (30 ng/ml) + IL-1beta (5 ng/ml)] was differentially enhanced by exposure to supplemental factors including LPS, L-arginine, and BH4. Nitrites 13-20 interferon gamma Homo sapiens 46-54 12009845-5 2002 The addition of IL-1 beta, TNF-alpha, and IFN-gamma together increased nitrite production: 257.5 +/- 35.8 % and S-nitrosothiol production : 413 +/- 29%, P < 0.001. Nitrites 71-78 interferon gamma Homo sapiens 42-51 11040335-3 2000 iNOS activity in a cell-free extract of lipopolysaccharide/interferon-gamma-stimulated RAW 264.7 cells was found to be markedly increased, and this increase was prevented by C-1 and C-2, accompanied by the parallel reduction in nitrite accumulation in culture medium. Nitrites 228-235 interferon gamma Homo sapiens 59-75 11751214-3 2002 Human bronchial epithelial cells stimulated with 50 ng/ml interleukin-1beta, tumor necrosis factor-alpha, and interferon-gamma express iNOS mRNA, protein and increased nitrite in the cell culture media, which was inhibited by the selective iNOS inhibitor 1400W. Nitrites 168-175 interferon gamma Homo sapiens 110-126 9573098-5 1998 When RAW264.7 cells were pretreated with human TNF or TNF(70-80) in the presence of IFN-gamma, there was a dose-dependent reduction in the replication of BCG as measured by the uptake of 3H-labeled uracil and a concomitant release of nitric oxide as measured by the nitrite in the culture supernatants. Nitrites 266-273 interferon gamma Homo sapiens 84-93 10800933-5 2000 The results demonstrate that KCI at 25-75 mM potently inhibits astrocyte nitrite production stimulated by interleukin-1 (IL-1)/interferon-gamma (IFNgamma). Nitrites 73-80 interferon gamma Homo sapiens 127-143 10800933-5 2000 The results demonstrate that KCI at 25-75 mM potently inhibits astrocyte nitrite production stimulated by interleukin-1 (IL-1)/interferon-gamma (IFNgamma). Nitrites 73-80 interferon gamma Homo sapiens 145-153 10648857-3 2000 Here, we have optimized a previously established test based on assessing the formation of neopterin or nitrite in interferon-gamma-treated human (THP-1) or murine (J774A.1, RAW264.7) monocytoid cell lines, respectively, in response to bacterial pyrogens. Nitrites 103-110 interferon gamma Homo sapiens 114-130 9691088-4 1998 The IL-1 receptor antagonist protein (IRAP) prevents TNF + LPS + IFN-gamma-induced iNOS expression and nitrite production, and attenuates the inhibitory effects on glucose-stimulated insulin secretion by human islets. Nitrites 103-110 interferon gamma Homo sapiens 65-74 10779013-3 2000 The NOS-2 activity, assessed by nitrite accumulation, was absent from untreated cultures but was induced by interleukin-1beta and interferon-gamma acting synergistically. Nitrites 32-39 interferon gamma Homo sapiens 130-146 10660117-3 2000 Treatment of P ll cells with interferon-gamma and lipopolysaccharide resulted in a 23-fold increase in nitrite production and induced expression of iNOS protein. Nitrites 103-110 interferon gamma Homo sapiens 29-45 10437653-5 1999 In addition, the co-application of G-CSF resulted in a decreased IFN-gamma/LPS mediated iNOS protein generation as detected by immunoblotting methods after 24 and 48 h. Measurement of the stable NO metabolites showed a significant reduction of nitrite/nitrate concentrations following co-incubation of VSMC with G-CSF + IFN-gamma/LPS (242.57 +/- 10.73 nmol NO2-/NO3-/mg cell protein, n = 8) as compared to IFN-gamma/LPS treatment (306.20 +/- 19.26 nmol NO2-/NO3-/mg cell protein, n = 8, P < 0.05) following a 24-h incubation protocol. Nitrites 244-251 interferon gamma Homo sapiens 65-74 10329044-7 1999 There was accumulation of nitrite in the culture medium of IFN-gamma-treated cells, suggesting the generation of NOx. Nitrites 26-33 interferon gamma Homo sapiens 59-68 11148756-2 1999 Our test system is based on the stimulation of IFN-gamma-primed human (THP-1) and murine (J774A.1 and RAW 264.7) monocytic cell lines by bacterial pyrogens to form neopterin or nitrite, respectively. Nitrites 177-184 interferon gamma Homo sapiens 47-56 10233688-3 1999 Interferon-gamma (IFN-gamma) completely overcame the enhancement of serotonin release and suppression of nitrite production induced by IL-4. Nitrites 105-112 interferon gamma Homo sapiens 0-16 10233688-3 1999 Interferon-gamma (IFN-gamma) completely overcame the enhancement of serotonin release and suppression of nitrite production induced by IL-4. Nitrites 105-112 interferon gamma Homo sapiens 18-27 10233688-4 1999 Over several experiments, with or without IL-4 and/or IFN-gamma, serotonin release correlated inversely with nitrite production. Nitrites 109-116 interferon gamma Homo sapiens 54-63 9747450-9 1998 Only interferon-gamma incubated alone was capable of increasing nitrite levels. Nitrites 64-71 interferon gamma Homo sapiens 5-21 8986132-8 1996 Ebselen prevented the increase in nitrite production by human islets exposed for 14 hr to a combination of cytokines (IL-1 beta, tumor necrosis factor-alpha and interferon-gamma). Nitrites 34-41 interferon gamma Homo sapiens 161-177 9191464-9 1997 Cell cultures treated with TNF alpha/IFN gamma/LPS in combination expressed iNOS mRNA, and this was associated with increases in supernatant nitrite concentrations over 24 h; however, this response diminished with successive passage of cells. Nitrites 141-148 interferon gamma Homo sapiens 37-46 8937711-12 1996 Addition of 2-amino-4-methylpyridine at the same time as the LPS and IFN-gamma, dose-dependently reduced the levels of nitrite (IC50 = 1.5 microM) without affecting the induction of NOS II protein. Nitrites 119-126 interferon gamma Homo sapiens 69-78 9126339-7 1997 IFN-gamma produced significant increases in medium levels of Epo and nitrite. Nitrites 69-76 interferon gamma Homo sapiens 0-9 9126339-9 1997 Furthermore, NG-nitro-L-arginine methyl ester (L-NAME), an NO synthase inhibitor, significantly decreased IFN-gamma induced elevations in medium levels of Epo and nitrite as well as cGMP levels in Hep3B cells. Nitrites 163-170 interferon gamma Homo sapiens 106-115 8985206-2 1997 Recently, generation of nitrite in culture supernatants of human macrophages exposed to interferon-gamma and interleukin-4 (IFN-gamma/IL-4) was reported. Nitrites 24-31 interferon gamma Homo sapiens 88-104 8985206-2 1997 Recently, generation of nitrite in culture supernatants of human macrophages exposed to interferon-gamma and interleukin-4 (IFN-gamma/IL-4) was reported. Nitrites 24-31 interferon gamma Homo sapiens 124-133 8985206-8 1997 Nitrite accumulation in experiments with IFN-gamma/IL-4 in human monocytes appears to be an in vitro artifact produced by nitrate-reducing activities contained in cytokine preparations. Nitrites 0-7 interferon gamma Homo sapiens 41-50 8923042-3 1996 Maximal plasma concentrations of nitrate plus nitrite were correlated to the degree of hypotension (r = -0.64, p = 0.02) and levels of tumor necrosis factor (TNF)-alpha (r = 0.51, p = 0.05) and soluble TNF receptors p55 and p75 (r = 0.58, p = 0.03 and r = 0.54, p = 0.04, respectively) but not to levels of interferon-gamma or interleukin-10 (p > 0.05). Nitrites 46-53 interferon gamma Homo sapiens 307-323 8784069-4 1996 The cytokine combination of interleukin-1 beta (50 U/mL), tumor necrosis factor-alpha (10(3) U/mL), and interferon-gamma (10(3) U/mL) induced significant increases in MDA and nitrite and significant decreases in insulin and DNA in islets after 60-h incubation. Nitrites 175-182 interferon gamma Homo sapiens 104-120 9064335-6 1996 Blood mononuclear cells from RA patients had increased NOS activity and increased NOS2 antigen content as compared to those from normal subjects, and responded to interferon-gamma with increased NOS expression and nitrite/nitrate production in vitro. Nitrites 214-221 interferon gamma Homo sapiens 163-179 8599841-2 1996 Schwann cells treated with IFN-gamma and TNF-alpha upregulated iNOS-specific mRNA within 12 hr and released nitrite in a time- and dose-dependent manner, reaching a plateau of secretion after 3 days. Nitrites 108-115 interferon gamma Homo sapiens 27-36 8760410-5 1996 Interferon gamma (IFN-gamma) stimulation however, resulted in reduced levels of nitrite accumulation as a direct consequence of Tat expression. Nitrites 80-87 interferon gamma Homo sapiens 0-27 8760410-6 1996 Conditioned media from Tat-expressing cells reduced the level of nitrite accumulation in parental cells following IFN-gamma stimulation but not stimulation with LPS. Nitrites 65-72 interferon gamma Homo sapiens 114-123 8603997-5 1996 Unlike murine macrophages, THP-1 cells produced little nitrite in response to interferon-gamma (IFN-gamma) and lipopolysaccharide, and a-MSH inhibited this production only slightly. Nitrites 55-62 interferon gamma Homo sapiens 78-94 8603997-5 1996 Unlike murine macrophages, THP-1 cells produced little nitrite in response to interferon-gamma (IFN-gamma) and lipopolysaccharide, and a-MSH inhibited this production only slightly. Nitrites 55-62 interferon gamma Homo sapiens 96-105 8782901-6 1996 Among the various cytokines investigated such as interleukin-1, interleukin-6, tumor necrosis factor-alpha and interferon-gamma (IFN-gamma), only IFN-gamma markedly enhanced A beta-dependent nitrite production. Nitrites 191-198 interferon gamma Homo sapiens 129-138 8782901-6 1996 Among the various cytokines investigated such as interleukin-1, interleukin-6, tumor necrosis factor-alpha and interferon-gamma (IFN-gamma), only IFN-gamma markedly enhanced A beta-dependent nitrite production. Nitrites 191-198 interferon gamma Homo sapiens 146-155 8590304-8 1995 Consistent with mammalian IFN-gamma, the nitrite-inducing activity was found to be heat labile, with over 90% of the activity lost within 5 minutes of heating. Nitrites 41-48 interferon gamma Homo sapiens 26-35 8680718-11 1995 8 The addition of TNF-alpha produced a significant (P < 0.001) 3 fold increase of IL-1 alpha/IFN-gamma-induced nitrite generation. Nitrites 114-121 interferon gamma Homo sapiens 96-105 8680718-7 1995 The combination of IL-1 alpha/IFN-gamma produced a highly significant (P < 0.001) 4 fold increase in nitrite production at 48 h, compared to basal values, while no other pair of cytokines was effective. Nitrites 104-111 interferon gamma Homo sapiens 30-39 8680718-8 1995 5 Time course studies with IL-1 alpha/IFN-gamma combination revealed significant (P < 0.001) increases in nitrite at 24 h (153 +/- 7), 48 h (306 +/- 24), and 72 h (384 +/- 15) compared to basal values of 50 +/- 4, 75 +/- 8, and 103 +/- 8 nM per 10(6) cells respectively. Nitrites 109-116 interferon gamma Homo sapiens 38-47 8680718-9 1995 6 Studies with IL-1 alpha/IFN-gamma combination demonstrated a time dependent expression of iNOS mRNA, first observed at 6 h, peaked at 24 h and was undetectable by 72 h. IL-1 alpha (0.3-10 ng ml-1) and IFN-gamma (10-300 u ml-1) in combination induced a concentration-dependent expression of iNOS mRNA at 24 h. 7 Pretreatment with cycloheximide before IL-1 alpha/IFN-gamma stimulation reduced nitrite levels to basal values. Nitrites 393-400 interferon gamma Homo sapiens 26-35 8680718-10 1995 These data suggest that the IL-1 alpha/IFN-gamma-induced nitrite production by HT-29 cells is dependent on de novo protein synthesis, probably the iNOS enzyme. Nitrites 57-64 interferon gamma Homo sapiens 39-48 8680718-13 1995 These findings suggest that the up-regulation by TNF-alpha of IL-1 alpha/IFN-gamma-induced nitrite generation is at the post-transcriptional level. Nitrites 91-98 interferon gamma Homo sapiens 73-82 7499964-8 1995 The formation of nitrites by interferon-gamma-activated macrophages was inhibited by TauCl in a dose-dependent manner. Nitrites 17-25 interferon gamma Homo sapiens 29-45 7542498-7 1995 Peritoneal macrophages have significantly enhanced nitrite/nitrate production and NOS activity after treatment with LPS and/or IFN-gamma, whereas monocyte nitrite/nitrate production and NOS activity are not altered by the treatments. Nitrites 51-58 interferon gamma Homo sapiens 127-136 8861714-1 1995 The combination of lipopolysaccharide (LPS; 100 ng/ml) and interferon-gamma (IFN-gamma; 10 IU/ml) synergistically stimulated induction of nitric oxide synthase activity in J774 macrophages, measured by nitrite accumulation during an overnight incubation. Nitrites 202-209 interferon gamma Homo sapiens 59-75 8861714-1 1995 The combination of lipopolysaccharide (LPS; 100 ng/ml) and interferon-gamma (IFN-gamma; 10 IU/ml) synergistically stimulated induction of nitric oxide synthase activity in J774 macrophages, measured by nitrite accumulation during an overnight incubation. Nitrites 202-209 interferon gamma Homo sapiens 77-86 7606802-5 1995 Preactivation of human monocytes with IFN-gamma led to subsequent increased IL-4- and CD23-driven nitrite and L-citrulline productions that were also suppressed in the presence of either nitro-L-arginine or the anti-CD23 mAb Fab fragment. Nitrites 98-105 interferon gamma Homo sapiens 38-47 7541323-9 1995 Concentrations of nitric oxide oxidation products, nitrite and nitrate, in incubation media were increased after exposure of cells to interferon-gamma. Nitrites 51-58 interferon gamma Homo sapiens 134-150 7530231-4 1994 The present data also indicated that addition of Interleukin-4 generally resulted in an increased nitrite production, that was potentiated by IFN-gamma, inactive alone. Nitrites 98-105 interferon gamma Homo sapiens 142-151 7693710-2 1993 In cultured vascular smooth muscle cells, interferon gamma (IFN-gamma) induced the accumulation of nitrite, a stable metabolite of nitric oxide, in a dose- and time-dependent manner. Nitrites 99-106 interferon gamma Homo sapiens 42-69 7514190-6 1994 Combinations of cytokines, notably IL-1 beta plus IFN-gamma plus TNF-alpha, induced increased expression of inducible NO synthase mRNA after 6 h and resulted in a fivefold increase in medium nitrite accumulation after 48 h. These cytokines did not impair glucose metabolism or insulin release in response to 16.7 mM glucose, but there was an 80% decrease in islet insulin content. Nitrites 191-198 interferon gamma Homo sapiens 50-59 7511593-9 1994 In addition, IL-4 induced an increased secretion of nitrite by monocytes, that was potentiated by IFN-gamma and inhibited by L-NMMA. Nitrites 52-59 interferon gamma Homo sapiens 98-107 7506700-2 1994 Interferon gamma, tumor necrosis factor-alpha, and interleukin-1 beta each markedly increased mRNA and protein levels of this enzyme in parallel with the production of nitrite, a stable oxidative metabolite of nitric oxide. Nitrites 168-175 interferon gamma Homo sapiens 0-45 1372867-1 1992 Activation of J774-macrophages with lipopolysaccharide (LPS) or LPS and recombinant interferon-gamma (IFN-gamma) induced nitric oxide (NO) synthase activity, as measured by the production of nitrite and citrulline. Nitrites 191-198 interferon gamma Homo sapiens 84-111 7689587-6 1993 The IL-1 beta-induced nitrite production by astrocytes was markedly enhanced when cells were co-stimulated with IFN-gamma or TNF-alpha (IFN-gamma > TNF-alpha); LPS had no effect used as a single agent or in combination with other cytokines. Nitrites 22-29 interferon gamma Homo sapiens 112-121 7689587-6 1993 The IL-1 beta-induced nitrite production by astrocytes was markedly enhanced when cells were co-stimulated with IFN-gamma or TNF-alpha (IFN-gamma > TNF-alpha); LPS had no effect used as a single agent or in combination with other cytokines. Nitrites 22-29 interferon gamma Homo sapiens 136-145 7680009-4 1993 Actinomycin D abolished the IFN gamma- and TNF alpha-induced increases in iNOS mRNA and nitrite production. Nitrites 88-95 interferon gamma Homo sapiens 28-37 7680009-5 1993 Cycloheximide, which abolished both the IFN gamma- and TNF alpha-induced increases in nitrite production, had no effect on the IFN gamma-induced increase in iNOS mRNA but markedly inhibited the TNF alpha-induced one. Nitrites 86-93 interferon gamma Homo sapiens 40-49