PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 14644171-6 2003 In contrast, both of these integrins bound to fibronectin-Sepharose. Sepharose 58-67 fibronectin 1 Homo sapiens 46-57 15253024-5 2004 Plasmatic FN was assessed by radial immunodiffusion with anti-human FN in 1% agarose gel slabs. Sepharose 77-84 fibronectin 1 Homo sapiens 10-12 22896898-1 2002 Limited proteolysis of buffalo plasma fibronectin (FN) by thermolysin yielded four gelatin-binding fragments of which, the major 59 kDa fragment, GBF1, was isolated by gelatin-Sepharose and heparin-Sepharose affinity columns. Sepharose 176-185 fibronectin 1 Homo sapiens 51-53 12862420-8 2003 To preserve the collagen-binding function of FN, it was pegylated while bound to a gelatin agarose matrix. Sepharose 91-98 fibronectin 1 Homo sapiens 45-47 22896898-1 2002 Limited proteolysis of buffalo plasma fibronectin (FN) by thermolysin yielded four gelatin-binding fragments of which, the major 59 kDa fragment, GBF1, was isolated by gelatin-Sepharose and heparin-Sepharose affinity columns. Sepharose 198-207 fibronectin 1 Homo sapiens 51-53 10470139-5 1999 Purification of the enzymatic activities using benzamidine sepharose yields a 50 kD and a 70 kD band of which the 50 kD band has fibronectin degrading capacity. Sepharose 59-68 fibronectin 1 Homo sapiens 129-140 11397124-11 2001 When the serum was passed over a gelatin-Sepharose column, which binds numerous proteins including fibronectin, the serum effect was lost. Sepharose 41-50 fibronectin 1 Homo sapiens 99-110 11150552-6 2001 As vitronectin and fibronectin each bind to heparin, these molecules are removed first and the heparin-Sepharose depletion occurs last in the sequence. Sepharose 103-112 fibronectin 1 Homo sapiens 19-30 11162401-10 2001 The four scFv antibody fragments bound specifically to ED-B-modified Sepharose and binding was further confirmed by immunofluorescence on cell cultures using ED-B-positive human Caco-2 tumor cells. Sepharose 69-78 fibronectin 1 Homo sapiens 55-59 11162401-10 2001 The four scFv antibody fragments bound specifically to ED-B-modified Sepharose and binding was further confirmed by immunofluorescence on cell cultures using ED-B-positive human Caco-2 tumor cells. Sepharose 69-78 fibronectin 1 Homo sapiens 158-162 9223381-6 1997 From a HT-29 cell lysate, only alphaVbeta6 bound to a fibronectin-Sepharose column. Sepharose 66-75 fibronectin 1 Homo sapiens 54-65 10331242-3 1999 In this context, the mineralization potential of fibronectin was tested in an agarose gel precipitation system and a metastable calcium phosphate solution. Sepharose 78-85 fibronectin 1 Homo sapiens 49-60 9653632-5 1998 In this study, fractionated acid-washed gelatin was cleaved with trypsin and resultant peptides fractionated by fibronectin-Sepharose affinity chromatography. Sepharose 124-133 fibronectin 1 Homo sapiens 112-123 8222162-5 1993 Endothelial cell fibronectin synthesis was determined after radiolabeling with [35S]-methionine in serum-free medium, gelatin-sepharose extraction of the culture medium and resolution on 5% SDS-PAGE. Sepharose 126-135 fibronectin 1 Homo sapiens 17-28 8743558-2 1996 Fibronectin receptor in several cell lysates was bound to a column of C279-immobilized Sepharose HP and obtained in a highly purified form by elution with a synthetic peptide, GRGDSP. Sepharose 87-96 fibronectin 1 Homo sapiens 0-11 8143454-1 1994 Fibronectin from human early pregnancy (5-8 weeks) placenta (epFN) has been isolated by 2M urea-PBS extraction and purified by heparin-Sepharose 4B affinity chromatography followed by Sepharose CL-6B gel filtration, and compared with that of term placenta (tpFN). Sepharose 135-144 fibronectin 1 Homo sapiens 0-11 9148599-1 1996 Human blood plasma fibronectin immobilised on agarose in physiological interacts with soluble myeloperoxidase (Kd = 2.43 mM). Sepharose 46-53 fibronectin 1 Homo sapiens 19-30 8557340-6 1996 65-kDa fibronectin-binding protein of M. penetrans was eluted following Sepharose-fibronectin affinity chromatography. Sepharose 72-81 fibronectin 1 Homo sapiens 7-18 8557340-6 1996 65-kDa fibronectin-binding protein of M. penetrans was eluted following Sepharose-fibronectin affinity chromatography. Sepharose 72-81 fibronectin 1 Homo sapiens 82-93 7578576-1 1995 Previous studies have demonstrated that fibronectin immobilized on BrCN-activated agarose forms a complex with soluble myeloperoxidase. Sepharose 82-89 fibronectin 1 Homo sapiens 40-51 7578576-3 1995 The thermodynamic characteristics of the myeloperoxidase interaction with fibronectin-gelatin-agarose have been established. Sepharose 94-101 fibronectin 1 Homo sapiens 74-85 8188759-11 1994 Further studies demonstrated that monoclonal antibody against the fibrin- and heparin-binding domain at the N-terminal of plasma fibronectin prevented radiolabeled hyaluronan from binding to fibronectin; likewise, the isolated N-terminal fragment, coupled with Sepharose 4B, bound to hyaluronan in columns. Sepharose 261-273 fibronectin 1 Homo sapiens 129-140 8138544-8 1993 Of these three proteoglycans, only CPGIIIB proteoglycan bound specifically to fibronectin-Sepharose 4B under physiological conditions. Sepharose 90-99 fibronectin 1 Homo sapiens 78-89 8138544-10 1993 Affinity chromatographies of the CPGIIIB proteoglycan on fibronectin-Sepharose 4B after treatments with these enzymes demonstrated that it bound to fibronectin via its heparan sulfate chains. Sepharose 69-78 fibronectin 1 Homo sapiens 57-68 8138544-10 1993 Affinity chromatographies of the CPGIIIB proteoglycan on fibronectin-Sepharose 4B after treatments with these enzymes demonstrated that it bound to fibronectin via its heparan sulfate chains. Sepharose 69-78 fibronectin 1 Homo sapiens 148-159 8370668-2 1993 When human plasma was applied to an alpha-elastin-Sepharose column at 4 degrees C, the column-binding fraction contained fibronectin. Sepharose 50-59 fibronectin 1 Homo sapiens 121-132 8352823-4 1993 Isolation and cross-linking of suitable fragments of fibronectin (relative molecular weights 65 and 52 kDa) to polylysine is followed by conjugation to gold thiomalate on a solid phase, gelatin-agarose affinity absorbent. Sepharose 194-201 fibronectin 1 Homo sapiens 53-64 8347617-1 1993 Chemical modification of plasma fibronectin (pFn) or its 40-kDa collagen/gelatin binding (CGB) domain by low concentrations of chloramine T (CT), a methionine-specific oxidant, caused decreased binding affinity between pFn or the isolated CGB domain and Sepharose-immobilized denatured collagen or a Texas Red-labeled CNBr fragment CB7 from the alpha 1 chain of type I collagen. Sepharose 254-263 fibronectin 1 Homo sapiens 32-43 8323285-1 1993 The specific interaction between fibronectin and collagen has permitted the isolation of fibronectin from plasma using gelatin-Sepharose affinity matrices. Sepharose 127-136 fibronectin 1 Homo sapiens 33-44 8323285-1 1993 The specific interaction between fibronectin and collagen has permitted the isolation of fibronectin from plasma using gelatin-Sepharose affinity matrices. Sepharose 127-136 fibronectin 1 Homo sapiens 89-100 8323285-4 1993 (ii) This tightly bound fibronectin could not be eluted from gelatin-Sepharose matrices with strong denaturing agents, such as 8.0 M urea or 6.0 M guanidium chloride. Sepharose 69-78 fibronectin 1 Homo sapiens 24-35 8323285-6 1993 (iv) Two fibronectin-derived fragments, CB52kDa and T55 kDa, selectively bound to fresh gelatin-Sepharose but not to gelatin-Sepharose previously employed to purify fibronectin, suggesting that these fragments recognize only the high affinity binding sites in gelatin. Sepharose 96-105 fibronectin 1 Homo sapiens 9-20 8501121-5 1993 Type I collagen carrying these substitutions bound weakly to fibronectin-sepharose and could be eluted off with 1 M urea. Sepharose 73-82 fibronectin 1 Homo sapiens 61-72 8370668-4 1993 However, the binding affinity of plasma fibronectin to the alpha-elastin-Sepharose column was much weaker at 25 degrees C than at 4 degrees C. The elastin-plasma fibronectin interaction was further confirmed by demonstrating the binding of alpha-elastin to fibronectin on polyvinylidene difluoride membranes using an alpha-elastin specific antibody. Sepharose 73-82 fibronectin 1 Homo sapiens 40-51 8370668-4 1993 However, the binding affinity of plasma fibronectin to the alpha-elastin-Sepharose column was much weaker at 25 degrees C than at 4 degrees C. The elastin-plasma fibronectin interaction was further confirmed by demonstrating the binding of alpha-elastin to fibronectin on polyvinylidene difluoride membranes using an alpha-elastin specific antibody. Sepharose 73-82 fibronectin 1 Homo sapiens 162-173 8370668-4 1993 However, the binding affinity of plasma fibronectin to the alpha-elastin-Sepharose column was much weaker at 25 degrees C than at 4 degrees C. The elastin-plasma fibronectin interaction was further confirmed by demonstrating the binding of alpha-elastin to fibronectin on polyvinylidene difluoride membranes using an alpha-elastin specific antibody. Sepharose 73-82 fibronectin 1 Homo sapiens 162-173 8486666-10 1993 The yield of VLA-5 fibronectin receptor bound to FN80-Sepharose columns was strongly increased upon treatment of U-937 cell lysates with mAb TS2/16. Sepharose 54-63 fibronectin 1 Homo sapiens 19-30 1434041-2 1992 Fibronectin was purified from blood bank homologous plasma by gelatin-Sepharose 4B affinity chromatography. Sepharose 70-82 fibronectin 1 Homo sapiens 0-11 1394043-5 1992 In addition, the immunosuppressive activity was bound to a gelatin-Sepharose affinity column, known to bind fibronectin. Sepharose 67-76 fibronectin 1 Homo sapiens 108-119 1928066-7 1991 IgA-fibronectin aggregates also were detected in serum using an antifibronectin antibody capture assay; and could be depleted from serum by heparin-agarose affinity chromatography. Sepharose 148-155 fibronectin 1 Homo sapiens 4-15 1616632-2 1992 After the cryogel was solubilized at 37 degrees C, 1:1 complex of fibrinogen and fibronectin was purified at room temperature by affinity chromatography on a gelatin-Sepharose 4B. Sepharose 166-175 fibronectin 1 Homo sapiens 81-92 1740645-4 1992 Plasma Fn was purified from the blood by gelatin-Sepharose affinity chromatography. Sepharose 49-58 fibronectin 1 Homo sapiens 7-9 2083241-6 1990 Fibronectin could also dissociate LDL-heparin complexes formed on heparin-Sepharose affinity columns. Sepharose 74-83 fibronectin 1 Homo sapiens 0-11 2214253-2 1990 The fibronectin was purified from autologous and homologous plasma by gelatin-Sepharose 4B affinity chromatography and administered topically, 500 micrograms/ml five times a day, for three weeks. Sepharose 78-87 fibronectin 1 Homo sapiens 4-15 2108022-2 1990 A novel hyperglycosylated fraction of human term fetal placental fibronectin was detected by long-term affinity binding to gelatin-Sepharose. Sepharose 131-140 fibronectin 1 Homo sapiens 65-76 1688916-3 1990 Melanoma-derived fibronectin was isolated from serum-free conditioned medium by gelatin-Sepharose affinity adsorption and shown to react with monoclonal antibodies HNK-1 and 10C5 in Western blot analysis. Sepharose 88-97 fibronectin 1 Homo sapiens 17-28 1688916-4 1990 HNK-1-containing fibronectin was purified on a gelatin-Sepharose column followed by an affinity column using a monoclonal antibody against the HNK-1 carbohydrate. Sepharose 55-64 fibronectin 1 Homo sapiens 17-28 2534245-1 1989 Plasmin, immobilized on Sepharose, was used for isolation of human blood plasma fibronectin fragments obtained after proteolysis. Sepharose 24-33 fibronectin 1 Homo sapiens 80-91 2534245-4 1989 These unbound to plasmin-Sepharose fibronectin fragments were found to stimulate proliferation of human embryonal fibroblasts in cell culture, whereas the plasmin-Sepharose bound peptides did not exhibit any effects on proliferation. Sepharose 25-34 fibronectin 1 Homo sapiens 35-46 2534245-2 1989 Under definite conditions the major part of the fibronectin fragments, liberated during proteolysis, remained to be bound to plasmin-Sepharose. Sepharose 133-142 fibronectin 1 Homo sapiens 48-59 2534619-1 1989 Electrophoretic purified fibronectin (FN) was isolated with high concentration (0.5 mg/ml plasma) from porcine plasma by affinity chromatography with gelatin-sepharose 4B and heparin-sepharose 4B. Sepharose 158-167 fibronectin 1 Homo sapiens 25-36 2534619-1 1989 Electrophoretic purified fibronectin (FN) was isolated with high concentration (0.5 mg/ml plasma) from porcine plasma by affinity chromatography with gelatin-sepharose 4B and heparin-sepharose 4B. Sepharose 158-167 fibronectin 1 Homo sapiens 38-40 2534619-1 1989 Electrophoretic purified fibronectin (FN) was isolated with high concentration (0.5 mg/ml plasma) from porcine plasma by affinity chromatography with gelatin-sepharose 4B and heparin-sepharose 4B. Sepharose 183-192 fibronectin 1 Homo sapiens 25-36 2534619-2 1989 The isolated FN shows one single band (MW 450,000) both in agarose gel and PAGE, and two similar bands (MW 230,000) in the presence of 1% beta-mercaptoethanol in SDS-PAGE. Sepharose 59-66 fibronectin 1 Homo sapiens 13-15 3220918-1 1988 Several problems are associated with the biospecific affinity purification of plasma fibronectin on gelatin-Sepharose. Sepharose 108-117 fibronectin 1 Homo sapiens 85-96 2624756-4 1989 Molecular sieve chromatography on Sephadex G-150 and affinity chromatography on gelatin-Sepharose revealed that there was one peak of chemotactic activity in high molecular weight range, which bound to gelatin, thus suggesting that the chemotactic factor might be fibronectin. Sepharose 88-97 fibronectin 1 Homo sapiens 264-275 2764118-4 1989 DEAE-Sephacyl ion exchange and gelatin-Sepharose affinity chromatography revealed two peaks containing chemotactic activity, one of which may be fibronectin, since it binds to gelatin, reacts in a specific immunoassay, and is inhibited of chemotactic activity by anti-fibronectin antiserum, and another of which does not appear to be fibronectin, since it does not bind to gelatin nor react in the immunoassay. Sepharose 39-48 fibronectin 1 Homo sapiens 145-156 3399792-1 1988 Fibronectin has been purified by gelatin-Sepharose affinity chromatography from fresh frozen human plasma. Sepharose 41-50 fibronectin 1 Homo sapiens 0-11 3178220-2 1988 We observed, however, that the elution of purified human plasma fibronectin from heparin-treated gelatin-agarose required the same high urea concentrations regardless of whether heparin treatment preceded or followed fibronectin adsorption. Sepharose 105-112 fibronectin 1 Homo sapiens 64-75 3403561-8 1988 Fibronectin was isolated from citrated human plasma by sequential gelatin-Sepharose affinity and DEAE ion-exchange chromatography in the presence of buffers containing 1 mM phenylmethylsulfonyl fluoride to prevent fragmentation. Sepharose 74-83 fibronectin 1 Homo sapiens 0-11 2847658-6 1988 Cathepsin D-digested placental fibronectin applied to a heparin-agarose column and eluted with a NaCl stepwise gradient (0.1, 0.3, 0.5 M) gave two polypeptides (80-100 and 65 kDa) in the 0.3 M NaCl peak. Sepharose 64-71 fibronectin 1 Homo sapiens 31-42 3178220-6 1988 We interpret these results as evidence that the stronger binding of fibronectin to gelatin-agarose in the presence of heparin is due to heparin itself binding to gelatin, thus allowing fibronectin to bind simultaneously to both immobilized ligands through appropriate domains of the glycoprotein. Sepharose 91-98 fibronectin 1 Homo sapiens 68-79 3178220-6 1988 We interpret these results as evidence that the stronger binding of fibronectin to gelatin-agarose in the presence of heparin is due to heparin itself binding to gelatin, thus allowing fibronectin to bind simultaneously to both immobilized ligands through appropriate domains of the glycoprotein. Sepharose 91-98 fibronectin 1 Homo sapiens 185-196 2969693-1 1988 A fibronectin binding protein (FnBp) was identified in 3H isoleucine labeled P. falciparum schizonts using affinity chromatography on human fibronectin (Fn) coupled to Sepharose 4B. Sepharose 168-177 fibronectin 1 Homo sapiens 2-13 2969693-1 1988 A fibronectin binding protein (FnBp) was identified in 3H isoleucine labeled P. falciparum schizonts using affinity chromatography on human fibronectin (Fn) coupled to Sepharose 4B. Sepharose 168-177 fibronectin 1 Homo sapiens 31-33 2969693-2 1988 After incubation of Nonidet-P 40 parasite lysate with Fn-Sepharose, elution was performed with SDS-PAGE buffer. Sepharose 57-66 fibronectin 1 Homo sapiens 54-56 3621887-2 1987 Fibronectin purified from humans plasma by affinity chromatography on gelatin-agarose and heparin-agarose was cleaved by thrombin into a 29,000 Dalton and a 210,000 Dalton fragments. Sepharose 78-85 fibronectin 1 Homo sapiens 0-11 2948581-8 1987 From octylglucoside extracts of radioactively surface-labeled cells, distinct Mr 190,000/185,000 membrane glycoproteins bound to Fn-heptapeptide-Sepharose, further suggesting that the Mr 190,000 polypeptide would be the Fn-receptor of the K562 cells. Sepharose 145-154 fibronectin 1 Homo sapiens 129-131 2448895-3 1987 The major contaminant fibronectin can be removed prior to the chromatography step by Gelatin-Sepharose adsorption. Sepharose 93-102 fibronectin 1 Homo sapiens 22-33 2822727-11 1987 A single membrane protein with the biochemical characteristics of the class I antigen was isolated on fibronectin-Sepharose and could be immunoprecipitated with the class I monoclonal antibody. Sepharose 114-123 fibronectin 1 Homo sapiens 102-113 3621887-2 1987 Fibronectin purified from humans plasma by affinity chromatography on gelatin-agarose and heparin-agarose was cleaved by thrombin into a 29,000 Dalton and a 210,000 Dalton fragments. Sepharose 98-105 fibronectin 1 Homo sapiens 0-11 2948272-0 1986 Phagocytosis of agarose beads by receptors for C3b (CR1) and iC3b (CR3) on human alveolar macrophages cultured on fibronectin in vitro. Sepharose 16-23 fibronectin 1 Homo sapiens 114-125 3768512-3 1986 Neutrophils were brought in contact with purified homologous fibronectin previously bound to gelatin-Sepharose granules. Sepharose 101-110 fibronectin 1 Homo sapiens 61-72 3098666-5 1986 Cross-immunoelectrophoresis of fulminant hepatic failure plasma for fibronectin on agarose plates gave an additional slower migrating peak in 15 of the 29 patients, as well as that of fibronectin, which corresponded to the fibronectin complex reported by other workers in leukemia. Sepharose 83-90 fibronectin 1 Homo sapiens 68-79 3024962-2 1986 The hybrid polypeptide was recognized by an anti-(human plasma fibronectin) serum and bound specifically to gelatin-Sepharose. Sepharose 116-125 fibronectin 1 Homo sapiens 63-74 3758208-3 1986 The suppression of contraction observed when fibronectin was eliminated from serum, either by affinity chromatography on gelatin-agarose columns or by precipitation with anti-fibronectin antibodies, showed that fibronectin is critical for the contraction. Sepharose 129-136 fibronectin 1 Homo sapiens 45-56 3811292-1 1986 Interaction of native and thrombin-modified human low density lipoproteins (LDL) with immobilized homologous fibronectin (either covalently bound to Sepharose or adsorbed from blood serum on collagen-Sepharose) was studied. Sepharose 149-158 fibronectin 1 Homo sapiens 109-120 3811292-3 1986 Chromatography of native and thrombinmodified LDL on fibronectin-Sepharose showed that 30% of the modified LDL and 2% of native LDL were bound to fibronectin-Sepharose at physiological pH values and NaCl concentrations. Sepharose 65-74 fibronectin 1 Homo sapiens 53-64 3811292-3 1986 Chromatography of native and thrombinmodified LDL on fibronectin-Sepharose showed that 30% of the modified LDL and 2% of native LDL were bound to fibronectin-Sepharose at physiological pH values and NaCl concentrations. Sepharose 158-167 fibronectin 1 Homo sapiens 146-157 3811292-4 1986 Study of the interaction of LDL with fibronectin adsorbed on collagen-Sepharose showed that thrombin-treated LDL partially released fibronectin from the sorbent due to the formation of a modified LDL-fibronectin complex. Sepharose 70-79 fibronectin 1 Homo sapiens 37-48 3768895-4 1986 The fibronectin glycopeptides were purified by gel-permeation chromatography and Con A-Sepharose and were analyzed by anion-exchange chromatography and affinity columns of immobilized 5-hydroxytryptamine and lectins. Sepharose 87-96 fibronectin 1 Homo sapiens 4-15 3532418-2 1986 One fragment of 60 kdaltons elutes from gelatin-Sepharose, after intact fibronectin, with pH 5.5, 50 mM citrate, 0.1 M NaCl. Sepharose 48-57 fibronectin 1 Homo sapiens 72-83 3525581-5 1986 Both of these antibodies were purified by affinity chromatography on columns of fibronectin-Sepharose and were then incubated with soluble fibronectin to form antigen-antibody complexes. Sepharose 92-101 fibronectin 1 Homo sapiens 80-91 3526144-7 1986 Fibronectin receptor was purified using affinity chromatography on human fibronectin coupled to Sepharose. Sepharose 96-105 fibronectin 1 Homo sapiens 0-11 3526144-9 1986 The purified fibronectin receptor was active since 40-60% of labeled receptor could rebind to fibronectin-Sepharose. Sepharose 106-115 fibronectin 1 Homo sapiens 13-24 3526144-9 1986 The purified fibronectin receptor was active since 40-60% of labeled receptor could rebind to fibronectin-Sepharose. Sepharose 106-115 fibronectin 1 Homo sapiens 94-105 3957921-6 1986 A ganglioside-Sepharose affinity column has been constructed which specifically binds the 31,000-dalton amino terminal fragment of fibronectin. Sepharose 14-23 fibronectin 1 Homo sapiens 131-142 3007451-5 1986 The gelatinase co-purified with fibronectin in chromatography on Sepharoses conjugated with gelatin, arginine, and heparin but could be separated from fibronectin by gel filtration in a physiological buffer. Sepharose 65-75 fibronectin 1 Homo sapiens 32-43 3948864-6 1986 On the other hand at pH 6.4 in conditions of physiological ionic strength, fibronectin was retained by both columns, eluting from the DNA-cellulose at 280 mM NaCl and from the heparin-Sepharose column at 210 mM. Sepharose 184-193 fibronectin 1 Homo sapiens 75-86 3946437-3 1986 Human plasma fibronectin was isolated by gelatin-Sepharose affinity chromatography and evaluated with respect to its opsonic activity following pasteurization, its in vivo clearance kinetics, and its short-term influence on cardiovascular hemodynamics in postoperative septic sheep. Sepharose 49-58 fibronectin 1 Homo sapiens 13-24 3948864-7 1986 Furthermore, we have studied the interaction of thermolysin-digested fibronectin both with DNA-cellulose and heparin-Sepharose using the above procedure. Sepharose 117-126 fibronectin 1 Homo sapiens 69-80 3983905-0 1985 Separation of plasma fibronectin from associated hemagglutinating activity by elution from gelatin-agarose at pH 5.5. Sepharose 99-106 fibronectin 1 Homo sapiens 21-32 3929855-1 1985 Interaction of human low-density lipoproteins (LDL) with homologous fibronectin fixed on collagen-Sepharose was studied. Sepharose 98-107 fibronectin 1 Homo sapiens 68-79 3929855-3 1985 Upon passing modified LDL through a fibronectin-collagen-Sepharose column the desorption of fibronectin occurred. Sepharose 57-66 fibronectin 1 Homo sapiens 36-47 3894594-4 1985 It binds to gelatin- and heparin-coupled Sepharose and it is recognized by specific anti-fibronectin sera. Sepharose 41-50 fibronectin 1 Homo sapiens 89-100 2861857-3 1985 Together with these heparin fractions, the following three series of heparin samples were examined to compare the affinity for fibronectin-Sepharose: four fractions separated on Sephadex G-100; five fractions separated on antithrombin III-Sepharose, and six partially and completely N-desulfated heparins. Sepharose 139-148 fibronectin 1 Homo sapiens 127-138 3872795-2 1985 By attaching native collagen and C1q to Sepharose, it was possible to test the binding of fibronectin (Fn) to the native and heat-denatured forms of these proteins without complications due to aggregation, precipitation, or fibril formation. Sepharose 40-49 fibronectin 1 Homo sapiens 90-101 3982800-2 1985 Fibronectin eyedrops were prepared from the patient"s own blood plasma by an affinity chromatography using gelatin-coupled agarose gel and by gel filtration technique. Sepharose 123-130 fibronectin 1 Homo sapiens 0-11 3929855-3 1985 Upon passing modified LDL through a fibronectin-collagen-Sepharose column the desorption of fibronectin occurred. Sepharose 57-66 fibronectin 1 Homo sapiens 92-103 4068572-1 1985 Fibronectin was isolated from a patient"s plasma by affinity chromatography using gelatin-agarose. Sepharose 90-97 fibronectin 1 Homo sapiens 0-11 3920213-7 1985 1) Upon urea gradient elution of affinity-bound fibronectin fragments from gelatin-Sepharose chromatography, the apex of the elution peak for polylactosamine-containing fragments occurs at 2.0 M urea while the peak for complex N-linked carbohydrate-containing fragments maximized at 2.5 M urea indicating a tighter binding. Sepharose 83-92 fibronectin 1 Homo sapiens 48-59 3983905-1 1985 Human plasma fibronectin prepared by elution of the adsorbed protein from gelatin-agarose by pH 5.5 citrate buffer is very low in hemagglutinating activity toward trypsinized rabbit erythrocytes, an activity previously associated with the purified plasma protein. Sepharose 82-89 fibronectin 1 Homo sapiens 13-24 6694247-6 1984 Labelled 75Se fibronectin was purified from donor rat plasma by gelatin-sepharose affinity chromatography. Sepharose 72-81 fibronectin 1 Homo sapiens 14-25 6489349-2 1984 Thrombospondin synthesized and secreted by human endothelial cells in culture binds specifically to fibronectin immobilized on Sepharose beads. Sepharose 127-136 fibronectin 1 Homo sapiens 100-111 6379853-1 1984 We have examined to what extent human fibronectin associated with agarose beads with a 5- to 10-micron diameter mediates binding and uptake of the beads by mouse macrophages and human monocytes. Sepharose 66-73 fibronectin 1 Homo sapiens 38-49 6379853-3 1984 When fibronectin was cross-linked to cyanogen bromide-activated agarose beads or incubated with gelatinized beads, this resulted in a significant increase in particle binding by macrophages and monocytes as compared with gelatinized beads, whereas the fraction of cells with ingested particles remained unaltered. Sepharose 64-71 fibronectin 1 Homo sapiens 5-16 6379853-6 1984 Since agarose beads are activators of the alternative pathway of complement, and fibronectin is reported to bind to factor C3, we speculate that cell-derived C3b is bound to the beads and fibronectin-coated beads are ingested by the phagocytes via complement C3b receptors on the cells. Sepharose 6-13 fibronectin 1 Homo sapiens 188-199 6198401-6 1984 Keratinocytes produced soluble fibronectin, since both immunoprecipitation and adsorption to gelatin-Sepharose detected 35S-methionine-labeled material which comigrated with human plasma fibronectin on sodium dodecyl sulfate polyacrylamide gels. Sepharose 101-110 fibronectin 1 Homo sapiens 31-42 6367010-7 1984 The results obtained with the two methods applied on a freshly prepared and a stored fibronectin are in agreement with sodium dodecylsulphate polyacrylamide gel electrophoresis followed by immunoblotting before and after gelatin-Sepharose adsorption. Sepharose 229-238 fibronectin 1 Homo sapiens 85-96 6367010-8 1984 These techniques demonstrate that all the freshly prepared fibronectin adsorbs to gelatin-Sepharose, while stored fibronectin, which is broken down to numerous peptides, still reacts with the fibronectin antibody, but does not adsorb to gelatin-Sepharose. Sepharose 90-99 fibronectin 1 Homo sapiens 59-70 6437941-3 1984 The major contaminants, fibronectin and IgM, were removed by affinity chromatography on gelatin- and anti-IgM-agarose, respectively. Sepharose 110-117 fibronectin 1 Homo sapiens 24-35 6735273-4 1984 When washed platelets, resuspended in a buffer containing fibronectin, were filtered on a low-affinity collagen-Sepharose, a significant increase in their adhesion occurred. Sepharose 112-121 fibronectin 1 Homo sapiens 58-69 6735273-5 1984 A similar modification could be observed when platelets were allowed to adhere to the same collagen-Sepharose preconditioned with fibronectin. Sepharose 100-109 fibronectin 1 Homo sapiens 130-141 6466396-5 1984 All normal and pathologic synovial fluid fibronectins showed a remarkably lower electrophoretic mobility compared with that of plasma fibronectin, when separated according to net molecular charge on agarose gel. Sepharose 199-206 fibronectin 1 Homo sapiens 41-52 6327694-8 1984 For example, one-half of the fibronectin-Sepharose-binding fraction from the long-term sites could also bind to platelet factor 4-Sepharose; however, over 90% of the fibronectin-binding fraction from newly formed sites could bind to platelet factor 4. Sepharose 41-50 fibronectin 1 Homo sapiens 29-40 6327694-8 1984 For example, one-half of the fibronectin-Sepharose-binding fraction from the long-term sites could also bind to platelet factor 4-Sepharose; however, over 90% of the fibronectin-binding fraction from newly formed sites could bind to platelet factor 4. Sepharose 130-139 fibronectin 1 Homo sapiens 29-40 6327694-9 1984 A major portion of the octyl-Sepharose-binding fractions of the original extracts could bind to fibronectin-Sepharose. Sepharose 29-38 fibronectin 1 Homo sapiens 96-107 6364982-5 1983 By contrast, a 42-kDa chymotrypsin-generated Fn fragment which retains the ability to adhere to gelatin-Sepharose exhibited normal (noncooperative) binding to both gelatin fractions with Kd = 7 X 10(-7) M. In all cases, the increase in P could be reversed by addition of excess unlabeled gelatin or urea. Sepharose 104-113 fibronectin 1 Homo sapiens 45-47 6643486-4 1983 Fractionation of the thermolysin digest of fibronectin on the glycosaminoglycan-Sepharoses at low ionic strength revealed that three groups of fragments, i.e. Mr = 150,000-140,000, 24,000, and 16,000 (150K-140K, 24K, and 16K) fragments, were capable of binding to glycosaminoglycans with different specificities and affinities. Sepharose 80-90 fibronectin 1 Homo sapiens 43-54 6643486-10 1983 At physiologic ionic strength, only heparin-Sepharose could bind intact fibronectin. Sepharose 44-53 fibronectin 1 Homo sapiens 72-83 6652976-9 1983 The opsonin was absorbed by immobilized Staphylococcus aureus and by gelatin-Sepharose, suggesting that it is related to fibronectin. Sepharose 77-86 fibronectin 1 Homo sapiens 121-132 6652976-10 1983 Fibronectin from normal human sera prepared by affinity chromatography on gelatin-Sepharose was not opsonic in this system. Sepharose 82-91 fibronectin 1 Homo sapiens 0-11 6661462-1 1983 Fibronectin isolated from bovine serum by affinity chromatography on collagen-Sepharose was found to contain a great number of concomitant proteins. Sepharose 78-87 fibronectin 1 Homo sapiens 0-11 6415800-1 1983 Fibronectin (FN) is a glycoprotein (disulfite-bonded dimer of 200 to 220 Kd submits) found in a soluble form in blood (concentration 250--500 microg/ml), it can be removed from it by cryoprecipitation and affinity chromatography on gelatin or heparin-agarose. Sepharose 251-258 fibronectin 1 Homo sapiens 0-11 6605399-5 1983 Affinity depletion of synovial fluid fibronectin resulted in loss of C1q and reduction in IgG in the cryoprotein; however, fibronectin, C1q, and IgG could not be co-eluted from affinity matrices of gelatin and protein A-Sepharose. Sepharose 220-229 fibronectin 1 Homo sapiens 37-48 6301464-2 1983 Fibronectin synthesized by HUH6 Cl5 was purified by gelatin-Sepharose affinity chromatography and compared with human plasma fibronectin in respect to molecular weight, electrophoretic mobility and antigenicity. Sepharose 60-69 fibronectin 1 Homo sapiens 0-11 6348922-6 1983 A third preparation of an apparently pure fibronectin has also been obtained by immuno-adsorption with a Sepharose 4B column to which has been coupled an anti-fibronectin monoclonal antibody. Sepharose 105-114 fibronectin 1 Homo sapiens 42-53 6602941-4 1983 Fibronectin bound tightly to gelatin-Sepharose and C1q-Sepharose and this binding could be inhibited by gelatin but not by C1q. Sepharose 37-46 fibronectin 1 Homo sapiens 0-11 6602941-4 1983 Fibronectin bound tightly to gelatin-Sepharose and C1q-Sepharose and this binding could be inhibited by gelatin but not by C1q. Sepharose 55-64 fibronectin 1 Homo sapiens 0-11 6289933-1 1982 Interaction between human neutrophils and fibronectin bound to gelatin-Sepharose granules was studied. Sepharose 71-80 fibronectin 1 Homo sapiens 42-53 6677247-2 1983 Plasma fibronectin was purified on gelatin-sepharose with 1 M arginine elution and iodinated with 125I by the solid phase glycoluril method. Sepharose 43-52 fibronectin 1 Homo sapiens 7-18 6834358-2 1983 Purification of plasma-opsonic fibronectin by affinity chromatography with gelatin-Sepharose revealed that although in vitro hepatic Kupffer cell phagocytosis was absolutely dependent upon the presence of fibronectin, the purified fibronectin evaluated in concentrations similar to that found in plasma (350-450 micrograms/ml) supported phagocytosis at a level two- to threefold less than that observed in whole plasma. Sepharose 83-92 fibronectin 1 Homo sapiens 31-42 7160472-0 1982 Interaction of fibronectin with arginine-agarose. Sepharose 41-48 fibronectin 1 Homo sapiens 15-26 7138504-3 1982 In affinity chromatography under physiological conditions, serum was depleted of fibronectin when run through columns of the carbodi-imide-treated proteins coupled to agarose. Sepharose 167-174 fibronectin 1 Homo sapiens 81-92 6749687-2 1982 Removal of the fibronectin by absorption of the plasma with agarose-immobilized gelatin abolished the attachment-promoting activity. Sepharose 60-67 fibronectin 1 Homo sapiens 15-26 7096593-3 1982 Fibronectin isolated from pleural fluid by affinity chromatography on gelatin-Sepharose had a polypeptide pattern similar to that of plasma fibronectin in SDS-polyacrylamide gel electrophoresis. Sepharose 78-87 fibronectin 1 Homo sapiens 0-11 6812233-1 1982 Simplified procedures have been developed for isolation of human plasma fibronectin by affinity chromatography on gelatin-agarose. Sepharose 122-129 fibronectin 1 Homo sapiens 72-83 6812233-3 1982 In a shorter process, fibronectin is eluted from gelatin-agarose simply by decreasing the buffer pH below 6. Sepharose 57-64 fibronectin 1 Homo sapiens 22-33 6337126-3 1983 Fibronectin covalently linked to agarose beads bound radiolabeled lipoteichoic acid in the acylated form but not in the deacylated form. Sepharose 33-40 fibronectin 1 Homo sapiens 0-11 6848519-4 1983 Labeled fibronectin was isolated from the culture media by gelatin-Sepharose chromatography, from cell surfaces by urea extraction, and from intracellular locations by cell lysis followed by immunoprecipitation. Sepharose 67-76 fibronectin 1 Homo sapiens 8-19 6288565-3 1982 In the present study, a fibronectin-binding protein was purified from sonicated S. aureus strain E2371 by affinity chromatography on fibronectin-Sepharose. Sepharose 145-154 fibronectin 1 Homo sapiens 24-35 6288565-3 1982 In the present study, a fibronectin-binding protein was purified from sonicated S. aureus strain E2371 by affinity chromatography on fibronectin-Sepharose. Sepharose 145-154 fibronectin 1 Homo sapiens 133-144 6288565-5 1982 The fibronectin-binding protein was released from fibronectin-Sepharose by carbamide (8 M). Sepharose 62-71 fibronectin 1 Homo sapiens 4-15 6288565-5 1982 The fibronectin-binding protein was released from fibronectin-Sepharose by carbamide (8 M). Sepharose 62-71 fibronectin 1 Homo sapiens 50-61 6289933-2 1982 It was found that fibronectin following cell-induced dissociation from gelatin-Sepharose caused neutrophil aggregation which was inhibited by EDTA or pretrypsinization of the cells. Sepharose 79-88 fibronectin 1 Homo sapiens 18-29 7068586-1 1982 The interaction of porcine plasma fibronectin and its proteolytic fragments with hyaluronic acid was investigated by affinity chromatography using hyaluronic acid-linked Sepharose 4B. Sepharose 170-179 fibronectin 1 Homo sapiens 34-45 6284125-2 1982 A fibronectin-binding protein as partially purified from washed solubilized human platelet membranes by affinity chromatography on fibronectin-Sepharose. Sepharose 143-152 fibronectin 1 Homo sapiens 2-13 6284125-2 1982 A fibronectin-binding protein as partially purified from washed solubilized human platelet membranes by affinity chromatography on fibronectin-Sepharose. Sepharose 143-152 fibronectin 1 Homo sapiens 131-142 7136937-5 1982 Human serum depleted of opsonic fibronectin by gelatin-sepharose affinity chromatography manifested a marked reduction in its ability to support phagocytosis of S aureus by human neutrophils. Sepharose 55-64 fibronectin 1 Homo sapiens 32-43 6757202-2 1982 The rabbit antihuman fibronectin was purified by affinity chromatography on human fibronectin-Sepharose. Sepharose 94-103 fibronectin 1 Homo sapiens 21-32 7082278-1 1982 The binding of fibronectin to gelatin-agarose was strictly dependent on pH, having a pH optimum of 7-9. Sepharose 38-45 fibronectin 1 Homo sapiens 15-26 6757202-2 1982 The rabbit antihuman fibronectin was purified by affinity chromatography on human fibronectin-Sepharose. Sepharose 94-103 fibronectin 1 Homo sapiens 82-93 7038987-1 1981 Fibronectin was isolated from human plasma by affinity chromatography with gelatin-coupled Sepharose 4B. Sepharose 91-100 fibronectin 1 Homo sapiens 0-11 7330549-0 1981 [Valve of a freeze-dried gelatin-agarose combination in selective extraction of fibronectin from human plasma]. Sepharose 33-40 fibronectin 1 Homo sapiens 80-91 7330549-1 1981 We report here a simple procedure for the isolation of human plasma fibronectin by affinity adsorption on a new lyophilized adsorbent which is obtained by mixing and polymerizing agarose with gelatin. Sepharose 179-186 fibronectin 1 Homo sapiens 68-79 7346232-2 1981 Matrices with excellent capacity and stability were obtained by coupling collagen-binding fragments of fibronectin to Sepharose. Sepharose 118-127 fibronectin 1 Homo sapiens 103-114 6457032-1 1981 Fibronectin was isolated from porcine plasma by affinity chromatography with gelatin-linked Sepharose 4B. Sepharose 92-101 fibronectin 1 Homo sapiens 0-11 6793685-5 1981 SAP, which had been aggregated either by direct conjugation to CNBr-activated Sepharose beads, or by complexing with anti-SAP antibodies immobilized on such beads, selectively took up fibronectin and C4-binding protein from whole normal human serum. Sepharose 78-87 fibronectin 1 Homo sapiens 184-195 6793685-7 1981 Experiments with isolated fibronectin and SAP complexed by anti-SAP-Sepharose indicated that close association of pairs of SAP molecules was required for fibronectin to be bound and that each SAP dimer was capable of taking up a single molecule of fibronectin. Sepharose 68-77 fibronectin 1 Homo sapiens 154-165 6793685-7 1981 Experiments with isolated fibronectin and SAP complexed by anti-SAP-Sepharose indicated that close association of pairs of SAP molecules was required for fibronectin to be bound and that each SAP dimer was capable of taking up a single molecule of fibronectin. Sepharose 68-77 fibronectin 1 Homo sapiens 154-165 7407251-1 1980 Collagen-fibronectin complexes, formed by binding of fibronectin to gelatin or collagen insolubilized on Sepharose, were found to bind 20-40% of radioactivity in [35S]heparin. Sepharose 105-114 fibronectin 1 Homo sapiens 9-20 6791940-3 1981 Fibronectin isolated from rheumatoid synovial fluid by affinity chromatography on gelatin--Sepharose had a polypeptide pattern similar to that of plasma fibronectin in SDS--polyacrylamide gel electrophoresis. Sepharose 91-100 fibronectin 1 Homo sapiens 0-11 28305355-1 1981 Fibronectin, with a subunit molecular weight of 220,000 daltons, was isolated from the ovary of the sea urchin,Pseudocentrotus depressus, using affinity chromatography on heat-denatured mammalian collagen coupled to Sepharose 4B. Sepharose 216-225 fibronectin 1 Homo sapiens 0-11 7264318-1 1981 Affinity chromatography of human plasma, using either gelatin or fibrinogen coupled to Sepharose 4B, depletes the plasma of fibronectin. Sepharose 87-99 fibronectin 1 Homo sapiens 124-135 7407251-1 1980 Collagen-fibronectin complexes, formed by binding of fibronectin to gelatin or collagen insolubilized on Sepharose, were found to bind 20-40% of radioactivity in [35S]heparin. Sepharose 105-114 fibronectin 1 Homo sapiens 53-64 7407251-2 1980 Fibronectin attached directly to Sepharose also bound [35S]heparin, while gelatin-Sepharose without fibronectin did not. Sepharose 33-42 fibronectin 1 Homo sapiens 0-11 7381215-3 1980 The procedure, based on the recently reported affinity of fibronectin for gelatin, essentially consists of two steps (1) Immunization of rabbits with fibronectin purified from serum by affinity chromatography using gelatin coupled to CNBr-activated Sepharose 4B. Sepharose 249-258 fibronectin 1 Homo sapiens 58-69 6893050-1 1980 Affinity chromatography with actin-Sepharose conjugates of purified human fibronectin, normal human plasma, or serum-free culture fluid from human fibroblasts showed that fibronectin molecules can directly bind to actin. Sepharose 35-44 fibronectin 1 Homo sapiens 74-85 6893050-1 1980 Affinity chromatography with actin-Sepharose conjugates of purified human fibronectin, normal human plasma, or serum-free culture fluid from human fibroblasts showed that fibronectin molecules can directly bind to actin. Sepharose 35-44 fibronectin 1 Homo sapiens 171-182 6893050-2 1980 A quantitative recovery of soluble human fibronectin was accomplished by chromatography on actin immobilized on Sepharose beads. Sepharose 112-121 fibronectin 1 Homo sapiens 41-52 6893050-3 1980 Human fibronectin molecules bound to actin-Sepharose were eluted with 0.25--0.35 M potassium bromide, and these molecules competed in a species-specific radioimmunoassay for human fibronectin. Sepharose 43-52 fibronectin 1 Homo sapiens 6-17 6893050-3 1980 Human fibronectin molecules bound to actin-Sepharose were eluted with 0.25--0.35 M potassium bromide, and these molecules competed in a species-specific radioimmunoassay for human fibronectin. Sepharose 43-52 fibronectin 1 Homo sapiens 180-191 6893050-4 1980 The subunits of fibronectin isolated by actin-Sepharose chromatography comigrated in SDS polyacrylamide gel electrophoresis with those of electrophoretically homogeneous fibronectin purified by conventional procedures. Sepharose 46-55 fibronectin 1 Homo sapiens 16-27 736901-1 1978 Soluble fibronectin of human plasma was specifically dissociated at neutral pH from gelatin-agarose by several cationic amino compounds, notably the polyamines spermine, spermidine and putrescine, the basic amino acid arginine, and amino sugars. Sepharose 92-99 fibronectin 1 Homo sapiens 8-19 372243-3 1979 Platelet and plasma fibronectin were bound and eluted from gelatin-sepharose under similar conditions. Sepharose 67-76 fibronectin 1 Homo sapiens 20-31 101631-4 1978 Fibronectin was isolated from human CSF by affinity chromatography on Sepharose-coupled gelatin and was further analyzed by SDS-polyacrylamide gel electrophoresis. Sepharose 70-79 fibronectin 1 Homo sapiens 0-11 20446669-3 2010 The electrode substrate patched with an agarose stamp having 50-microm-high bumps was used for the spatially confined detachment of heparin/polyethyleneimine precoated on glass substrates, followed by micropatterned adsorption of fibronectin. Sepharose 40-47 fibronectin 1 Homo sapiens 230-241 355597-6 1978 The radioactive fibronectin present in the endothelial postculture medium and in urea extracts of cellular monolayers was isolated with either anti-fibronectin coupled to Protein A-Sepharose or double antibody immunoprecipitation and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 181-190 fibronectin 1 Homo sapiens 16-27 567240-1 1978 Fibronectin, a fibroblast surface protein, was purified from human and chicken plasma and extracts of cultured chicken fibroblasts with affinity chromatography on gelatin coupled to Sepharose particles. Sepharose 182-191 fibronectin 1 Homo sapiens 0-11 31463436-5 2018 We report that preculture of NP cells in agarose gels was required in order for cells to be mechanoresponsive, and this correlated with increased type VI collagen, alpha5beta1 integrin, and fibronectin expression. Sepharose 41-48 fibronectin 1 Homo sapiens 190-201 23485472-5 2013 Pull down analysis using SSL8-conjugated Sepharose and recombinant truncated fragments of TNC revealed that SSL8 interacts with fibronectin (FN) type III repeats 1-5 of TNC. Sepharose 41-50 fibronectin 1 Homo sapiens 128-139 22104730-6 2012 The MMP-9 production from monocytes was diminished by the depletion of fibronectin from the conditioned media with gelatin-Sepharose, and potentiated by culturing them in fibronectin-coated plates. Sepharose 123-132 fibronectin 1 Homo sapiens 71-82 903179-6 1977 Preparative affinity chromatography of plasma on gelatin coupled to Sepharose gave electrophoretically and immunologically pure fibronectin in high yields. Sepharose 68-77 fibronectin 1 Homo sapiens 128-139 1158872-2 1975 CI globulin was distinguished from antihemophilic factor (AHF) by amino acid analysis, position of elution from 4% agarose, and electrophoretic migration in polyacrylamide gels in the presence of sodium dodecyl sulfate without prior reduction. Sepharose 115-122 fibronectin 1 Homo sapiens 0-11 25293592-5 2015 RESULTS: SDS-agarose immunoblotting of patients" plasma revealed the presence of several additional FN-fibrin bands, with decreasing electrophoretic mobilities and increasing molecular masses of 750 kDa, 1000 kDa, 1300 kDa, 1600 kDa and 1900 kDa. Sepharose 13-20 fibronectin 1 Homo sapiens 100-102 25394993-5 2015 FN is PEGylated while it is bound to gelatin Sepharose beads with 2, 5, and 10 kDa PEG precursors. Sepharose 45-54 fibronectin 1 Homo sapiens 0-2 20580823-4 2010 Distribution of collagen type I and fibronectin in the ECM of the agarose cultures was determined by immunoflorescence. Sepharose 66-73 fibronectin 1 Homo sapiens 36-47 19909400-2 2010 Preparations of FN purified from human plasma by gelatin-Sepharose affinity chromatography typically also contain gelatin-binding gelatinases that may cleave FN, reduce its stability and alter its biological activities. Sepharose 57-66 fibronectin 1 Homo sapiens 16-18 19909400-5 2010 MATERIAL AND METHODS: Experiments tested the elution profiles for FN and gelatinases from gelatin-Sepharose using a concentration range (1-7%) of dimethyl sulfoxide (DMSO) and 4 m urea as eluants. Sepharose 98-107 fibronectin 1 Homo sapiens 66-68 18677626-0 2009 Dynamic compression inhibits fibronectin fragment induced iNOS and COX-2 expression in chondrocyte/agarose constructs. Sepharose 99-106 fibronectin 1 Homo sapiens 29-40