PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24862437-0 2014 Selective purification of supercoiled p53-encoding pDNA with L-methionine-agarose matrix. Sepharose 74-81 tumor protein p53 Homo sapiens 38-41 27651634-8 2016 The present study of p53 gene regulation analyzed the expression of 279-bp bands on 1.5 agarose gel. Sepharose 88-95 tumor protein p53 Homo sapiens 21-24 26520021-8 2015 The support l-tyrosine Sepharose used in chromatographic experiments promotes the separation of native pVAX1-LacZ and pcDNA3-FLAG-p53 samples (oc+sc) by decreasing the salt concentration. Sepharose 23-32 tumor protein p53 Homo sapiens 130-133 15842803-2 2005 METHODS: Four different p53-GST (glutathione S-transferase) fusion proteins and GST were expressed in E. coli and purified through glutathione sepharose 4B beads. Sepharose 143-152 tumor protein p53 Homo sapiens 24-27 16426567-2 2006 Using a competition assay on agarose gels we found that the p53 consensus sequences in longer DNA fragments are better targets than the same sequences in shorter DNAs. Sepharose 29-36 tumor protein p53 Homo sapiens 60-63 22901138-2 2012 METHODS: Stool DNA was isolated and tumor-associated high molecular weight DNA (1.476 kb fragment including exons 6-9 of the p53 gene) was amplified using PCR and visualized on ethidium bromide-stained agarose gels. Sepharose 202-209 tumor protein p53 Homo sapiens 125-128 11258898-6 2001 Activated MAPK immunoprecipitates of nuclei from T(4)-treated cells accumulated p53 in a time-dependent manner; T(4) and T(4)-agarose were more effective than T(3). Sepharose 126-133 tumor protein p53 Homo sapiens 80-83 14711380-7 2004 Duplication time, plating efficiency, colony formation in agarose, and contact inhibition were also altered in the p53 mutated tumor cultures compared to those carrying wild-type p53. Sepharose 58-65 tumor protein p53 Homo sapiens 115-118 12694871-4 2003 Binding of active, latent, and in vitro-activated p53 protein to DNA fragments modified by antitumor cisplatin was studied using electrophoretic mobility shift assay in agarose gels and immunoblotting analysis. Sepharose 169-176 tumor protein p53 Homo sapiens 50-53 10673394-2 2000 Supershifting of p53-DNA complexes by MAbs in agarose gels was applied to studies of activation of p53 for sequence-specific binding within scDNA. Sepharose 46-53 tumor protein p53 Homo sapiens 17-20 11168396-2 2001 Significant differences were observed; p53CD produced a relatively small and continuous retardation of scDNA, in contrast to the ladder of distinct bands formed by p53 in agarose gels. Sepharose 171-178 tumor protein p53 Homo sapiens 164-167 10673394-2 2000 Supershifting of p53-DNA complexes by MAbs in agarose gels was applied to studies of activation of p53 for sequence-specific binding within scDNA. Sepharose 46-53 tumor protein p53 Homo sapiens 99-102 10533721-6 1999 Agarose gel electrophoresis confirmed the AdCMV.p53-dependent cellular apoptosis. Sepharose 0-7 tumor protein p53 Homo sapiens 48-51 10432310-1 1999 The ability to separate the isoforms of human tumour suppressor protein p53 expressed in insect cells using heparin-Sepharose correlates with differences in the isoelectric point of p53, demonstrating that p53 can be heterogeneously modified and providing support for the use of insect cells as a model system for identifying novel signalling pathways that target p53. Sepharose 116-125 tumor protein p53 Homo sapiens 72-75 9393978-1 1997 Wild type human tumor suppressor protein p53 (expressed in insect cells) binds strongly to negatively supercoiled (sc) plasmid DNA at a native superhelix density, as evidenced by electrophoretic retardation of scDNA in agarose gels and imaging by scanning force microscopy (SFM). Sepharose 219-226 tumor protein p53 Homo sapiens 41-44 35377359-6 2022 Total cellular Flag-tagged p53 protein was purified with anti-Flag antibody-conjugated agarose under nondenaturing conditions. Sepharose 87-94 tumor protein p53 Homo sapiens 27-30 34711005-7 2021 The PCR products of all the exons of TP53 (Exons 2 to 11) were electrophoresed on agarose gel, purified and sequenced by Sanger method. Sepharose 82-89 tumor protein p53 Homo sapiens 37-41 7957681-3 1994 A recombinant human wild type p53 fused with glutathione S-transferase was immobilized on glutathione-agarose as a ligand for affinity column. Sepharose 102-109 tumor protein p53 Homo sapiens 30-33 29334217-3 2018 Using a combination of nuclear magnetic resonance (NMR) titration, isothermal titration calorimetry, fluorescence anisotropy, and native agarose gel electrophoresis, we have identified a direct interaction between the p53 DBD and Hsp90 co-chaperone p23 that occurs in the absence of Hsp90. Sepharose 137-144 tumor protein p53 Homo sapiens 218-221 29334217-5 2018 We show by NMR and native agarose gel electrophoresis that a p53-specific double-stranded DNA sequence competes successfully with p23 for binding to the p53 DBD. Sepharose 26-33 tumor protein p53 Homo sapiens 61-64 29334217-5 2018 We show by NMR and native agarose gel electrophoresis that a p53-specific double-stranded DNA sequence competes successfully with p23 for binding to the p53 DBD. Sepharose 26-33 tumor protein p53 Homo sapiens 153-156