PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 26478185-7 2016 Insulin release from 10% (w/w) implants into agitated solution was faster as compared to release into agarose hydrogel. Sepharose 102-109 insulin Homo sapiens 0-7 7876259-6 1995 We used the soluble recombinant insulin receptor kinase domain, which phosphorylated hIRS-p30 with high affinity (Km = 11.9 +/- 0.8 microM), and affinity columns prepared by coupling hIRS-p30 to NHS-activated Sepharose for binding assays. Sepharose 209-218 insulin Homo sapiens 32-39 11785967-2 2002 The insulin derivative was prepared by treatment of insulin-Sepharose with ammonium bicarbonate. Sepharose 60-69 insulin Homo sapiens 4-11 11785967-2 2002 The insulin derivative was prepared by treatment of insulin-Sepharose with ammonium bicarbonate. Sepharose 60-69 insulin Homo sapiens 52-59 8916921-2 1996 Here we describe methods for the purification of an insulin-stimulated insulin receptor serine kinase from human placenta and rat liver by sequential chromatography of solubilized membranes on wheat germ agglutinin-agarose, Mono Q, phenyl-Superose, and Superose 12. Sepharose 215-222 insulin Homo sapiens 52-59 25604495-6 2015 In a subgroup of 20 HIV positive and 5 donors, binding of plasma insulin to sIR was determined by ELISA assay of residual insulin levels in plasma immuno-depleted with anti-IR-monoclonal antibody-Sepharose beads. Sepharose 196-205 insulin Homo sapiens 65-72 9636226-4 1998 Insulin also increased by severalfold the 32P content of mTOR that was determined after purifying the protein from 32P-labeled adipocytes with rapamycin.FKBP12 agarose beads. Sepharose 160-167 insulin Homo sapiens 0-7 9228925-10 1997 Constant production and delivery of proinsulin could be achieved by encapsulating the human insulin cDNA-transfected cells using 5% agarose. Sepharose 132-139 insulin Homo sapiens 36-46 9228925-10 1997 Constant production and delivery of proinsulin could be achieved by encapsulating the human insulin cDNA-transfected cells using 5% agarose. Sepharose 132-139 insulin Homo sapiens 39-46 7877136-2 1994 Detergent-solubilized insulin receptors from IM-9 cells immobilized on Sepharose beads by 960 antisera bound 2-3 times more 125I-insulin tracer (25-60 pM) than receptors immobilized with either 83-7 or CT-1. Sepharose 71-80 insulin Homo sapiens 22-29 8145772-5 1993 This inhibitory activity copurifies with the IR on insulin-Sepharose affinity chromatography and is also effective against the tyrosine kinase activity of the IR-related insulin-like growth factor-I receptor and the oncoprotein v-abl but is ineffective against c-src tyrosine kinase activity. Sepharose 59-68 insulin Homo sapiens 51-58 8069230-5 1994 IR and Gir from human placental membrane bound to insulin-Sepharose column. Sepharose 58-67 insulin Homo sapiens 50-57 8145772-5 1993 This inhibitory activity copurifies with the IR on insulin-Sepharose affinity chromatography and is also effective against the tyrosine kinase activity of the IR-related insulin-like growth factor-I receptor and the oncoprotein v-abl but is ineffective against c-src tyrosine kinase activity. Sepharose 59-68 insulin Homo sapiens 170-177 2480780-3 1989 The highly purified receptors (prepared by insulin-Sepharose chromatography) were 5-10 times more effective in catalysing the phosphorylation of calmodulin than an equal number of partially purified receptors (prepared by wheat-germ agglutinin-Sepharose chromatography). Sepharose 51-60 insulin Homo sapiens 43-50 1918001-2 1991 By using insulin that has been protected in positions A1 and B29, we have been able to couple the insulin selectively through the B1 amino group to divinyl sulfone-activated agarose. Sepharose 174-181 insulin Homo sapiens 9-16 1918001-2 1991 By using insulin that has been protected in positions A1 and B29, we have been able to couple the insulin selectively through the B1 amino group to divinyl sulfone-activated agarose. Sepharose 174-181 insulin Homo sapiens 98-105 1824821-3 1991 Insulin binding activity was purified from Triton X-100 solubilized membranes in two steps: FPLC on a MonoQ HR5/5 column; and affinity chromatography on insulin-agarose. Sepharose 161-168 insulin Homo sapiens 0-7 1824821-3 1991 Insulin binding activity was purified from Triton X-100 solubilized membranes in two steps: FPLC on a MonoQ HR5/5 column; and affinity chromatography on insulin-agarose. Sepharose 161-168 insulin Homo sapiens 153-160 1499862-4 1992 We obtained a specific antiproinsulin antibody by absorbing the initial goat antiserum with human C-peptide-agarose. Sepharose 108-115 insulin Homo sapiens 98-107 1645756-6 1991 Kinase activity, determined with receptors immobilized on insulin agarose beads, was measured at 0.5 microM ATP, with 1 mg/ml histone, followed by SDS-PAGE. Sepharose 66-73 insulin Homo sapiens 58-65 2136851-1 1990 In these studies we demonstrate that insulin stimulates both tyrosine and serine phosphorylation of the insulin receptor after its partial purification on wheat germ-agarose, and after affinity purification on insulin-agarose. Sepharose 166-173 insulin Homo sapiens 37-44 2136851-1 1990 In these studies we demonstrate that insulin stimulates both tyrosine and serine phosphorylation of the insulin receptor after its partial purification on wheat germ-agarose, and after affinity purification on insulin-agarose. Sepharose 166-173 insulin Homo sapiens 104-111 2136851-1 1990 In these studies we demonstrate that insulin stimulates both tyrosine and serine phosphorylation of the insulin receptor after its partial purification on wheat germ-agarose, and after affinity purification on insulin-agarose. Sepharose 166-173 insulin Homo sapiens 104-111 2136851-1 1990 In these studies we demonstrate that insulin stimulates both tyrosine and serine phosphorylation of the insulin receptor after its partial purification on wheat germ-agarose, and after affinity purification on insulin-agarose. Sepharose 218-225 insulin Homo sapiens 37-44 2136851-1 1990 In these studies we demonstrate that insulin stimulates both tyrosine and serine phosphorylation of the insulin receptor after its partial purification on wheat germ-agarose, and after affinity purification on insulin-agarose. Sepharose 218-225 insulin Homo sapiens 104-111 2136851-1 1990 In these studies we demonstrate that insulin stimulates both tyrosine and serine phosphorylation of the insulin receptor after its partial purification on wheat germ-agarose, and after affinity purification on insulin-agarose. Sepharose 218-225 insulin Homo sapiens 104-111 3058126-7 1988 A synthetic peptide containing residues 243-251 was specifically bound by agarose-insulin beads. Sepharose 74-81 insulin Homo sapiens 82-89 2665407-5 1989 Prior to determination of proinsulin-binding IgG, the insulin-binding IgG was removed by means of sepharose-bound insulin according to the method of Heding. Sepharose 98-107 insulin Homo sapiens 54-61 3277840-4 1988 Purification of adipocyte insulin receptors by wheat germ agglutinin-Sepharose chromatography, followed by insulin-agarose affinity chromatography, resulted in loss of DTT stimulation of insulin binding. Sepharose 69-78 insulin Homo sapiens 26-33 3277840-4 1988 Purification of adipocyte insulin receptors by wheat germ agglutinin-Sepharose chromatography, followed by insulin-agarose affinity chromatography, resulted in loss of DTT stimulation of insulin binding. Sepharose 115-122 insulin Homo sapiens 26-33 3277840-4 1988 Purification of adipocyte insulin receptors by wheat germ agglutinin-Sepharose chromatography, followed by insulin-agarose affinity chromatography, resulted in loss of DTT stimulation of insulin binding. Sepharose 115-122 insulin Homo sapiens 107-114 3277840-4 1988 Purification of adipocyte insulin receptors by wheat germ agglutinin-Sepharose chromatography, followed by insulin-agarose affinity chromatography, resulted in loss of DTT stimulation of insulin binding. Sepharose 115-122 insulin Homo sapiens 107-114 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 106-113 insulin Homo sapiens 0-7 3023795-2 1986 Both antisera were adsorbed against human C-peptide conjugated to Sepharose, following which cross-reactivity to insulin and C-peptide was less than 0.001%. Sepharose 66-75 insulin Homo sapiens 42-51 3023795-2 1986 Both antisera were adsorbed against human C-peptide conjugated to Sepharose, following which cross-reactivity to insulin and C-peptide was less than 0.001%. Sepharose 66-75 insulin Homo sapiens 113-120 3023795-2 1986 Both antisera were adsorbed against human C-peptide conjugated to Sepharose, following which cross-reactivity to insulin and C-peptide was less than 0.001%. Sepharose 66-75 insulin Homo sapiens 125-134 2881538-5 1986 The effect of insulin on acetyl-CoA carboxylase activity, but not the effect on phosphorylation, was lost on purification of the enzyme on avidin-Sepharose. Sepharose 146-155 insulin Homo sapiens 14-21 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 172-179 insulin Homo sapiens 0-7 3304287-2 1987 Using a SH-free glutathione-agarose column it is demonstrated that the interaction of insulin with glutathione is specific, and increasing the incubation time between these two peptides results in the reduction of insulin disulfide bonds and the production of A and B chains. Sepharose 28-35 insulin Homo sapiens 86-93 3304287-2 1987 Using a SH-free glutathione-agarose column it is demonstrated that the interaction of insulin with glutathione is specific, and increasing the incubation time between these two peptides results in the reduction of insulin disulfide bonds and the production of A and B chains. Sepharose 28-35 insulin Homo sapiens 214-221 3553193-3 1987 In pulse-chase experiments with [35S] methionine, labeled receptor species were separated into "active" and "inactive" forms by affinity chromatography on insulin-agarose and then were characterized and quantitated. Sepharose 163-170 insulin Homo sapiens 155-162 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 172-179 insulin Homo sapiens 118-125 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 172-179 insulin Homo sapiens 283-290 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 172-179 insulin Homo sapiens 0-7 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 172-179 insulin Homo sapiens 118-125 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 172-179 insulin Homo sapiens 283-290 2419331-2 1986 8-Bromo-cAMP (1 mM) or forskolin (10 microM) enhanced the phosphorylation of the insulin receptor purified from 32P-labeled cells by affinity chromatography on wheat germ agglutinin-agarose and immunoprecipitation with monoclonal antibody. Sepharose 182-189 insulin Homo sapiens 81-88 6325418-4 1984 Both activities are bound to the same extent on insulin-Sepharose, and the immobilized kinase, after extensive washing, exhibits activity versus histone, which closely approaches that of the insulin-stimulated, solubilized kinase. Sepharose 56-65 insulin Homo sapiens 48-55 2859355-9 1985 The human BBB plasma membranes were solubilized in Triton X-100 and were adsorbed to a wheat germ agglutinin Sepharose affinity column, indicating the BBB insulin receptor is a glycoprotein. Sepharose 109-118 insulin Homo sapiens 155-162 6390435-1 1984 The ligand N alpha, B1-(6-biotinylamido)hexanoyl-insulin was attached noncovalently to Sepharose 4B immobilized succinoylavidin to form an insulin-affinity resin. Sepharose 87-99 insulin Homo sapiens 49-56 6390435-1 1984 The ligand N alpha, B1-(6-biotinylamido)hexanoyl-insulin was attached noncovalently to Sepharose 4B immobilized succinoylavidin to form an insulin-affinity resin. Sepharose 87-99 insulin Homo sapiens 139-146 6203118-4 1984 The purified receptor was eluted from insulin-Sepharose with 0.5 M NaCl and 1 mM dithiothreitol at pH 5.5. Sepharose 46-55 insulin Homo sapiens 38-45 4074346-3 1985 Insulin receptors in Triton X-100-solubilized microsomal membranes were purified 2,000-fold by sequential affinity chromatography on wheat germ lectin-agarose and insulin-CDI-activated agarose. Sepharose 151-158 insulin Homo sapiens 0-7 4074346-3 1985 Insulin receptors in Triton X-100-solubilized microsomal membranes were purified 2,000-fold by sequential affinity chromatography on wheat germ lectin-agarose and insulin-CDI-activated agarose. Sepharose 185-192 insulin Homo sapiens 0-7 4074346-3 1985 Insulin receptors in Triton X-100-solubilized microsomal membranes were purified 2,000-fold by sequential affinity chromatography on wheat germ lectin-agarose and insulin-CDI-activated agarose. Sepharose 185-192 insulin Homo sapiens 163-170 3919966-0 1985 Immunoenzymometric assay for insulin involving column chromatography and insulin immobilized on Sepharose. Sepharose 96-105 insulin Homo sapiens 29-36 3919966-0 1985 Immunoenzymometric assay for insulin involving column chromatography and insulin immobilized on Sepharose. Sepharose 96-105 insulin Homo sapiens 73-80 6325418-4 1984 Both activities are bound to the same extent on insulin-Sepharose, and the immobilized kinase, after extensive washing, exhibits activity versus histone, which closely approaches that of the insulin-stimulated, solubilized kinase. Sepharose 56-65 insulin Homo sapiens 191-198 6309774-4 1983 The extent of phosphorylation of insulin and somatomedin-C receptors was assessed by immunoprecipitating the wheat germ agglutinin Sepharose eluates with monoclonal antibodies specific for each receptor and analyzing the immunoprecipitates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 131-140 insulin Homo sapiens 33-40 6309826-1 1983 The insulin receptor purified from human placenta by sequential affinity chromatography on wheat germ agglutinin- and insulin-Sepharose to near homogeneity retained tyrosine-specific protein kinase activity. Sepharose 126-135 insulin Homo sapiens 4-11 6313160-5 1983 We have observed that both receptors were adsorbed by affinity columns of insulin-agarose, in a manner that did not yield insulin receptor entirely free from the BSM receptor. Sepharose 82-89 insulin Homo sapiens 74-81 6406486-1 1983 A glycoprotein-enriched fraction derived from 3T3-L1 adipocyte membranes by Triton X-100 extraction and chromatography on wheat germ agglutinin-agarose contains an insulin-activable protein kinase that catalyzes the phosphorylation of tyrosine residues in proteins (Petruzzelli, L. M., Ganguly, S., Smith, C.J., Cobb, M. H., Rubin, C.S., and Rosen, O. M. (1982) Proc. Sepharose 144-151 insulin Homo sapiens 164-171 6574482-4 1983 The time required for activation is consistent with that needed for insulin-dependent self-phosphorylation of the receptor present in eluates from wheat germ lectin-agarose columns and in preparations of affinity-purified placental receptor. Sepharose 165-172 insulin Homo sapiens 68-75 7047272-2 1982 Insulin specific IgE and IgG are bound to insulin covalently coupled to Sepharose particles. Sepharose 72-81 insulin Homo sapiens 0-7 6188161-1 1983 Highly purified human placental insulin receptors were obtained by sequential affinity chromatography on wheat germ agglutinin and insulin-agarose. Sepharose 139-146 insulin Homo sapiens 32-39 6188161-1 1983 Highly purified human placental insulin receptors were obtained by sequential affinity chromatography on wheat germ agglutinin and insulin-agarose. Sepharose 139-146 insulin Homo sapiens 131-138 7152133-7 1982 The solubilized photolabeled insulin receptor fraction was enriched by first adsorbing to agarose-bound wheat germ agglutinin and the material eluted with N-acetyl-D-glucosamine was then analyzed by SDS-PAGE and autoradiography. Sepharose 90-97 insulin Homo sapiens 29-36 6833290-2 1983 Sepharose CL-6B column chromatography of the 125I-insulin-receptor complex obtained by both of the above procedures yielded a highly radioactive 140,000-Da complex which was dissociated into small peptides when subjected to SDS-polyacrylamide gel electrophoresis. Sepharose 0-9 insulin Homo sapiens 50-57 6833291-2 1983 The receptor was eluted from insulin-Sepharose using mild conditions, eliminating urea, so that it was stable and retained full insulin-binding activity. Sepharose 37-46 insulin Homo sapiens 29-36 6833291-2 1983 The receptor was eluted from insulin-Sepharose using mild conditions, eliminating urea, so that it was stable and retained full insulin-binding activity. Sepharose 37-46 insulin Homo sapiens 128-135 6833291-6 1983 In comparison, the receptor eluted from insulin-Sepharose with previously used conditions in the presence of urea resulted in maximum insulin binding of only 6 micrograms per mg of protein. Sepharose 48-57 insulin Homo sapiens 40-47 6833291-6 1983 In comparison, the receptor eluted from insulin-Sepharose with previously used conditions in the presence of urea resulted in maximum insulin binding of only 6 micrograms per mg of protein. Sepharose 48-57 insulin Homo sapiens 134-141 6294652-5 1982 The insulin-activated kinase is copurified with the human placental insulin receptor until the final elution from insulin-Sepharose. Sepharose 122-131 insulin Homo sapiens 4-11 6294652-5 1982 The insulin-activated kinase is copurified with the human placental insulin receptor until the final elution from insulin-Sepharose. Sepharose 122-131 insulin Homo sapiens 68-75 7002370-0 1980 Enzyme immunoassay for insulin with a novel separation method using activated thiol-Sepharose. Sepharose 84-93 insulin Homo sapiens 23-30 6940121-3 1981 Insulin receptors were concentrated and purified 20-fold by chromatography of the cell extract on wheat germ agglutinin-agarose, and then specifically precipitated by receptor antibodies after addition of a second antibody. Sepharose 120-127 insulin Homo sapiens 0-7 557070-1 1977 Insulin was directly coupled and also indirectly coupled by a side chain to Sepharose 4B to form immunoabsorbents for affinity chromatography of insulin antibodies. Sepharose 76-88 insulin Homo sapiens 0-7 288074-5 1979 Sulfitolysis of highly purified material to break the inter- and intra-chain disulfide bridges and subsequent adsorption on a specific B-chain antibody covalently bound to Sepharose beads showed that the C-peptide was still connected to the B-chain. Sepharose 172-181 insulin Homo sapiens 204-213 403392-8 1977 The very recent work from the laboratory of Heding, however, has brought about major advances in this area in which human C-peptide and proinsulin can be separated in the plasma by the use of Sepharose particles. Sepharose 192-201 insulin Homo sapiens 122-131 403392-8 1977 The very recent work from the laboratory of Heding, however, has brought about major advances in this area in which human C-peptide and proinsulin can be separated in the plasma by the use of Sepharose particles. Sepharose 192-201 insulin Homo sapiens 136-146 557070-1 1977 Insulin was directly coupled and also indirectly coupled by a side chain to Sepharose 4B to form immunoabsorbents for affinity chromatography of insulin antibodies. Sepharose 76-88 insulin Homo sapiens 145-152 958454-0 1976 Combination of insulin chains on an anti-insulin antibody-Sepharose column. Sepharose 58-67 insulin Homo sapiens 15-22 1138860-6 1975 Whereas treatment of Con A- (and insulin-) derivatized beads with anti-insulin antiserum, and cells with trypsin, readily inhibited binding of insulin-Sepharose to cells, neither treatment inhibited Con A-Sepharose binding. Sepharose 151-160 insulin Homo sapiens 33-40 1239396-4 1975 Insulin antibodies coupled to Sepharose were used to bind human proinsulin and insulin in the serum and after centrifugation C-peptide was determined in the supernatant. Sepharose 30-39 insulin Homo sapiens 64-74 1239396-4 1975 Insulin antibodies coupled to Sepharose were used to bind human proinsulin and insulin in the serum and after centrifugation C-peptide was determined in the supernatant. Sepharose 30-39 insulin Homo sapiens 67-74 1138860-6 1975 Whereas treatment of Con A- (and insulin-) derivatized beads with anti-insulin antiserum, and cells with trypsin, readily inhibited binding of insulin-Sepharose to cells, neither treatment inhibited Con A-Sepharose binding. Sepharose 151-160 insulin Homo sapiens 71-78 1138860-6 1975 Whereas treatment of Con A- (and insulin-) derivatized beads with anti-insulin antiserum, and cells with trypsin, readily inhibited binding of insulin-Sepharose to cells, neither treatment inhibited Con A-Sepharose binding. Sepharose 151-160 insulin Homo sapiens 71-78 1055365-0 1975 Structure of a soluble super-active insulin is revealed by the nature of the complex between cyanogen-bromide-activated sepharose and amines. Sepharose 120-129 insulin Homo sapiens 36-43 1055365-1 1975 Insulin-like material with elevated insulin specific acitivity is released from insulin-Sepharose in the presence of bovine-serum albumin. Sepharose 88-97 insulin Homo sapiens 0-7 1055365-1 1975 Insulin-like material with elevated insulin specific acitivity is released from insulin-Sepharose in the presence of bovine-serum albumin. Sepharose 88-97 insulin Homo sapiens 36-43 1055365-1 1975 Insulin-like material with elevated insulin specific acitivity is released from insulin-Sepharose in the presence of bovine-serum albumin. Sepharose 88-97 insulin Homo sapiens 80-87 4461581-0 1974 Proceedings: Critical evaluation of sepharose-coupled insulin as a tool for studies of insulin action at the membrane level. Sepharose 36-45 insulin Homo sapiens 54-61 1054501-0 1975 Re-evaluation of Sepharose-insulin as a tool for the study of insulin action. Sepharose 17-26 insulin Homo sapiens 27-34 1054501-0 1975 Re-evaluation of Sepharose-insulin as a tool for the study of insulin action. Sepharose 17-26 insulin Homo sapiens 62-69 1054501-1 1975 The biological activity of Sepharose-insulin in different assays in vitro, e.g., stimulation of glucose oxidation, lipogenesis, and antilipolysis and activation of pyruvate dehydrogenase (EC 1.2.4.1) activity, has been investigated. Sepharose 27-36 insulin Homo sapiens 37-44 1054501-5 1975 After incubation with tissue or cells, Sepharose-insulin particles were separated by centrifugation from the medium. Sepharose 39-48 insulin Homo sapiens 49-56 1054501-7 1975 A quantitative evaluation of the soluble biological and immunological insulin activity in the supernatant accounted for the total insulin activity of Sepharose-insulin. Sepharose 150-159 insulin Homo sapiens 70-77 1054501-7 1975 A quantitative evaluation of the soluble biological and immunological insulin activity in the supernatant accounted for the total insulin activity of Sepharose-insulin. Sepharose 150-159 insulin Homo sapiens 130-137 4461581-0 1974 Proceedings: Critical evaluation of sepharose-coupled insulin as a tool for studies of insulin action at the membrane level. Sepharose 36-45 insulin Homo sapiens 87-94 4689220-0 1973 Biological activity of insulin-sepharose? Sepharose 31-40 insulin Homo sapiens 23-30 5570426-1 1971 Stimulation of glycogen synthetase in an in vitro liver system by insulin bound to sepharose. Sepharose 83-92 insulin Homo sapiens 66-73 4504339-3 1972 Several insulin-agarose derivatives have been synthesized that can efficiently extract the insulin-binding protein from the detergent extracts of the membranes. Sepharose 16-23 insulin Homo sapiens 8-15 4504339-3 1972 Several insulin-agarose derivatives have been synthesized that can efficiently extract the insulin-binding protein from the detergent extracts of the membranes. Sepharose 16-23 insulin Homo sapiens 91-98 4351804-1 1973 Immobilized insulin, prepared by coupling insulin directly to agarose or through hydrocarbon "connecting arms," was demonstrated to be capable of firmly binding intact adipocytes and their ghosts. Sepharose 62-69 insulin Homo sapiens 12-19 4351804-2 1973 Various lines of evidence indicate that the insulin receptor on the plasma membrane, in addition to the insulin coupled to the agarose, was responsible for the observed binding. Sepharose 127-134 insulin Homo sapiens 44-51 4351804-3 1973 This evidence includes: (a) the finding that increasing the "arm" length increased the binding capacities of insulin-Sepharose affinity chromatographic columns, (b) specific inhibition and reversal by insulin and antiserum to insulin of the binding, as compared to lesser effects by other peptide hormones, (c) the indication that only the plasma membrane sacs, not the other cellular contaminants in the crude ghosts, are capable of binding, and (d) the impairment and restoration of trypsin-sensitive membrane binding sites that are also required for insulin biosensitivity. Sepharose 117-126 insulin Homo sapiens 109-116 4351804-3 1973 This evidence includes: (a) the finding that increasing the "arm" length increased the binding capacities of insulin-Sepharose affinity chromatographic columns, (b) specific inhibition and reversal by insulin and antiserum to insulin of the binding, as compared to lesser effects by other peptide hormones, (c) the indication that only the plasma membrane sacs, not the other cellular contaminants in the crude ghosts, are capable of binding, and (d) the impairment and restoration of trypsin-sensitive membrane binding sites that are also required for insulin biosensitivity. Sepharose 117-126 insulin Homo sapiens 201-208 4351804-3 1973 This evidence includes: (a) the finding that increasing the "arm" length increased the binding capacities of insulin-Sepharose affinity chromatographic columns, (b) specific inhibition and reversal by insulin and antiserum to insulin of the binding, as compared to lesser effects by other peptide hormones, (c) the indication that only the plasma membrane sacs, not the other cellular contaminants in the crude ghosts, are capable of binding, and (d) the impairment and restoration of trypsin-sensitive membrane binding sites that are also required for insulin biosensitivity. Sepharose 117-126 insulin Homo sapiens 201-208 4351804-3 1973 This evidence includes: (a) the finding that increasing the "arm" length increased the binding capacities of insulin-Sepharose affinity chromatographic columns, (b) specific inhibition and reversal by insulin and antiserum to insulin of the binding, as compared to lesser effects by other peptide hormones, (c) the indication that only the plasma membrane sacs, not the other cellular contaminants in the crude ghosts, are capable of binding, and (d) the impairment and restoration of trypsin-sensitive membrane binding sites that are also required for insulin biosensitivity. Sepharose 117-126 insulin Homo sapiens 201-208 4351804-5 1973 By use of the differential buoyant densities of the various cell-bead complexes that resulted from the interaction of adipocytes with insulin-Sepharose, a new procedure was developed to demonstrate and study the binding. Sepharose 142-151 insulin Homo sapiens 134-141 5437344-0 1970 Immunological reactivity of insulin to sepharose coupled with insulin-antibody---its use for the extraction of insulin from serum. Sepharose 39-48 insulin Homo sapiens 28-35 5483637-0 1970 Stimulation of RNA synthesis in isolated mammary cells by insulin and prolactin bound to sepharose. Sepharose 89-98 insulin Homo sapiens 58-65 5257136-1 1969 Insulin can be covalently attached to a large polymers of Sepharose through the alpha-amino group of the N-terminal residue of the B chain, or through the epsilon-amino group of its lysyl residue. Sepharose 58-67 insulin Homo sapiens 0-7 5257136-3 1969 The effects occur with concentrations of insulin-Sepharose that are nearly as low as those of native insulin, and the maximal responses are the same. Sepharose 49-58 insulin Homo sapiens 41-48 5815208-0 1969 Insulin-sepharose: immunoreactivity and use in the purification of antibody. Sepharose 8-17 insulin Homo sapiens 0-7 33439347-2 2021 In this study, a mammosphere model has been characterised using human mammary epithelial cells grown on either mouse extracellular matrix or agarose and showed insulin is essential for formation of mammospheres. Sepharose 141-148 insulin Homo sapiens 160-167