PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 33440481-2 2016 These cationic glycopolymers were found to form very stable polyplexes with EGFR siRNA as determined by dynamic light scattering and agarose gel electrophoresis. Sepharose 133-140 epidermal growth factor Homo sapiens 76-80 12905882-2 2001 METHOD: Chemically synthesized hEGF gene was expressed in Yeast Pichia pastoris and the secretory hEGF was purified by Phenysepharose 6 Fast Flow(high sub), Q-sepharose High Performance, and Superdex 30 chromatography, and its characters were studied by respective methods. Sepharose 124-133 epidermal growth factor Homo sapiens 31-35 12905882-2 2001 METHOD: Chemically synthesized hEGF gene was expressed in Yeast Pichia pastoris and the secretory hEGF was purified by Phenysepharose 6 Fast Flow(high sub), Q-sepharose High Performance, and Superdex 30 chromatography, and its characters were studied by respective methods. Sepharose 124-133 epidermal growth factor Homo sapiens 98-102 7714662-2 1995 In this study, we purified the hEGF sample using Benzamidine-Sepharose 6B column chromatography as a critical step for purification and newly developed an EIA system with specificity for hEGF. Sepharose 61-70 epidermal growth factor Homo sapiens 31-35 10920955-2 1998 METHODS: The growth and gene expression of HCE16/3 cells treated with IGF-I and epidermal growth factor (EGF) were examined by flow cytometry, soft-agarose assay and RT-PCR analysis. Sepharose 148-155 epidermal growth factor Homo sapiens 80-103 10920955-2 1998 METHODS: The growth and gene expression of HCE16/3 cells treated with IGF-I and epidermal growth factor (EGF) were examined by flow cytometry, soft-agarose assay and RT-PCR analysis. Sepharose 148-155 epidermal growth factor Homo sapiens 105-108 10920955-6 1998 Furthermore, the combined use of EGF and IGF-I could induce colony formation of HCE16/3 cells in soft agarose. Sepharose 102-109 epidermal growth factor Homo sapiens 33-36 7750206-2 1995 In this report we show that EGF promotes the malignant behavior of the Moser cells in vitro in terms of growth in soft agarose and invasion of Matrigel-coated porous membranes. Sepharose 119-126 epidermal growth factor Homo sapiens 28-31 1639209-4 1992 Preliminary characterisation has excluded EGF and TGF alpha, and demonstrated that the activity is bound reversibly by heparin-Sepharose, thus pointing to a member of the heparin-binding fibroblast- or hepatocyte-growth factor families. Sepharose 127-136 epidermal growth factor Homo sapiens 42-45 3496043-3 1987 HMW hEGF bound poorly to concanavalin A-agarose, quite avidly to wheat germ lectin-agarose, and completely to phenyl boronate-agarose, suggesting that it was O-glycosylated. Sepharose 83-90 epidermal growth factor Homo sapiens 4-8 2808426-7 1989 Combined with column chromatography of the solubilized EGF receptor on protamine-agarose gel, these results suggest that protamine may increase the EGF receptor number by directly activating cryptic EGF receptors in the plasma membrane. Sepharose 81-88 epidermal growth factor Homo sapiens 55-58 1847663-8 1991 The cellular growth response of DiFi cells to exogenous EGF was studied in monolayer cultures as well as in a soft agarose assay. Sepharose 115-122 epidermal growth factor Homo sapiens 56-59 3496043-3 1987 HMW hEGF bound poorly to concanavalin A-agarose, quite avidly to wheat germ lectin-agarose, and completely to phenyl boronate-agarose, suggesting that it was O-glycosylated. Sepharose 83-90 epidermal growth factor Homo sapiens 4-8 3079907-7 1986 As with pp35, EGF(Uro)-stimulated phosphorylation of isolated rabbit liver beta-35 was observed in a reconstituted system using either EDTA/EGTA-washed placental membranes or solubilized EGF(Uro) receptor immobilized on concanavalin A-agarose. Sepharose 235-242 epidermal growth factor Homo sapiens 14-22 6378914-1 1984 The characteristics of the insulin- and epidermal growth factor (EGF)-stimulated tyrosine-specific protein kinases in a wheat germ lectin-Sepharose-purified preparation of solubilized placenta membranes were compared. Sepharose 138-147 epidermal growth factor Homo sapiens 65-68 3001106-0 1985 Receptors for insulin and epidermal growth factor: interaction with organomercurial agarose. Sepharose 84-91 epidermal growth factor Homo sapiens 14-49 6330113-5 1984 The 32P content of the EGF receptor purified with EGF-Sepharose was increased by 38% compared with the same amount of receptor isolated from control cells. Sepharose 54-63 epidermal growth factor Homo sapiens 23-26 6330113-5 1984 The 32P content of the EGF receptor purified with EGF-Sepharose was increased by 38% compared with the same amount of receptor isolated from control cells. Sepharose 54-63 epidermal growth factor Homo sapiens 50-53 33514813-7 2021 Although both of the immobilized samples show dose-dependency when seeded with high number of fibroblast cells, CNBr-agarose-EGF showed a significantly high activity at 100 ng/mL and 72 h incubation, compared to glyoxyl-agarose-EGF. Sepharose 117-124 epidermal growth factor Homo sapiens 125-128 33514813-7 2021 Although both of the immobilized samples show dose-dependency when seeded with high number of fibroblast cells, CNBr-agarose-EGF showed a significantly high activity at 100 ng/mL and 72 h incubation, compared to glyoxyl-agarose-EGF. Sepharose 117-124 epidermal growth factor Homo sapiens 228-231 219006-3 1979 The hEGF/hUG in homogenates of human salivary glands and a wide variety of other endocrine and nonendocrine tissues was extracted by Amberlite CG-50 cation exchange chromatography and immune affinity chromatography using the immunoglobulin fraction of rabbit anti-hEGF serum covalently bound to agarose. Sepharose 295-302 epidermal growth factor Homo sapiens 4-8