PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15814629-3 2005 Amplified ACE gene fragments were separated on agarose gels. Sepharose 47-54 angiotensin I converting enzyme Homo sapiens 10-13 18756878-5 2008 The genotyping of polymorphism in the intron 16 of the angiotensin-converting enzyme was performed by the polymerase chain reaction followed by the agarose electrophoresis. Sepharose 148-155 angiotensin I converting enzyme Homo sapiens 55-84 19130799-5 2008 ACE insertion/deletion polymorphism was determined by agarose gel electrophoresis and D/D typing was further reconfirmed using insertion-allele-specific amplification. Sepharose 54-61 angiotensin I converting enzyme Homo sapiens 0-3 12903404-2 2000 METHODS: The experiment was carried out under the low water activity condition, using tosylate chloride activating side-chain hydroxyl group of Sepharose CL-4B agarose to form a high active group which could react with the free amino-group of ACE to link the enzyme with agarose. Sepharose 160-167 angiotensin I converting enzyme Homo sapiens 243-246 15677791-6 2005 The I/D polymorphism of the ACE gene was examined by PCR followed by agarose gel electrophoresis. Sepharose 69-76 angiotensin I converting enzyme Homo sapiens 28-31 12901825-2 2003 METHODS: ACE I/D polymorphism was performed on DNA samples from patients using the polymerase chain reaction (PCR) followed by agarose gel electrophoresis. Sepharose 127-134 angiotensin I converting enzyme Homo sapiens 9-12 12442545-4 2002 Genotypes of ACE gene were determined by polymeraze chain reaction, followed with electrophoresis in agarose gel. Sepharose 101-108 angiotensin I converting enzyme Homo sapiens 13-16 15033618-6 2004 Angiotensin converting enzyme genotypes were determined by agarose gel sizing after polymerase chain reaction (PCR) amplification. Sepharose 59-66 angiotensin I converting enzyme Homo sapiens 0-29 12903404-2 2000 METHODS: The experiment was carried out under the low water activity condition, using tosylate chloride activating side-chain hydroxyl group of Sepharose CL-4B agarose to form a high active group which could react with the free amino-group of ACE to link the enzyme with agarose. Sepharose 271-278 angiotensin I converting enzyme Homo sapiens 243-246 11035682-7 2000 ACE genotyping was performed on DNA samples from patients using the polymerase chain reaction followed by agarose gel electrophoresis. Sepharose 106-113 angiotensin I converting enzyme Homo sapiens 0-3 9693941-7 1998 ACE genotypes were determined by agarose gel sizing after polymerase chain reaction (PCR) amplification. Sepharose 33-40 angiotensin I converting enzyme Homo sapiens 0-3 10459873-5 1999 METHODS: Maternal and fetal samples were genotyped at the insertion-deletion (I-D) polymorphism in intron 16 of the angiotensin-converting enzyme gene by the polymerase chain reaction followed by agarose electrophoresis. Sepharose 196-203 angiotensin I converting enzyme Homo sapiens 116-145 10430974-3 1999 The ACE insertion/deletion (I/D) polymorphism was amplified with the previously published flanking primers, and the polymerase chain reaction product was separated, sized on a 2% agarose gel, and visualized by ultraviolet transillumination. Sepharose 179-186 angiotensin I converting enzyme Homo sapiens 4-7 10354208-4 1999 Alleles of an insertion/deletion polymorphism of the ACE gene were determined by one-stage polymerase chain reaction and visualized on agarose gels. Sepharose 135-142 angiotensin I converting enzyme Homo sapiens 53-56 10023114-4 1999 ACE (I/D) genotype was determined using polymerase chain reaction and 3% agarose gel electrophoresis. Sepharose 73-80 angiotensin I converting enzyme Homo sapiens 0-3 9178346-1 1997 A soluble 65582.9 Da (in MALDI-TOF) angiotensin converting (ACE)-like enzyme has been purified by a captopril-Sepharose affinity column chromatography from the mollusk Mytilus edulis. Sepharose 110-119 angiotensin I converting enzyme Homo sapiens 60-63 2558510-5 1989 We purified one ACE from the soluble fraction by lisinopril-linked Sepharose 6B affinity column chromatography and Cellulofine GCL-200 gel filteration. Sepharose 67-76 angiotensin I converting enzyme Homo sapiens 16-19 8052664-4 1994 Column chromatography, including modified affinity chromatography on lisinopril-Sepharose, yielded homogeneous ACE after only a 45-fold purification. Sepharose 80-89 angiotensin I converting enzyme Homo sapiens 111-114 35271257-6 2022 As a demonstration, we showed that ACE I/D could be genotyped using a low amount of DNA sample (25 ng) with an accuracy of 100%, without complicated operation steps and data analysis, which is better than that of the conventional method (agarose gel electrophoresis analysis after common PCR). Sepharose 238-245 angiotensin I converting enzyme Homo sapiens 35-38 26782563-3 2015 The ACE InDel polymorphism was genotyped by polymerase chain reaction (PCR) with specific primers, followed by electrophoresis on 1.5% agarose gel. Sepharose 135-142 angiotensin I converting enzyme Homo sapiens 4-7 2999099-2 1985 Angiotensin-converting enzyme was purified from human lung, kidney, testis, blood plasma, and seminal plasma using a facile two-step protocol which included affinity chromatography on Sepharose-bound lisinopril followed by either gel filtration or hydroxylapatite chromatography. Sepharose 184-193 angiotensin I converting enzyme Homo sapiens 0-29 23601467-5 2013 PCR and agarose gel electrophoresis methods were adopted to determine the rs1799752 I/D polymorphism genotypes of the ACE gene. Sepharose 8-15 angiotensin I converting enzyme Homo sapiens 118-121 21477733-5 2011 The ACE fragment containing the polymorphism was amplified using conventional polymerase chain reaction using specific primer pairs and subsequently genotyped using agarose gel electrophoresis. Sepharose 165-172 angiotensin I converting enzyme Homo sapiens 4-7