PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 8136702-2 1993 MIF specifically bound glutathione (dissociation constant = 600 microM), and the protein was effectively purified by an S-hexylglutathione Sepharose affinity column. Sepharose 139-148 macrophage migration inhibitory factor Homo sapiens 0-3 9560321-1 1998 Macrophage-migration-inhibitory factor (MIF) is retained by S-hexylglutathione-agarose but is not specifically eluted in high yield. Sepharose 79-86 macrophage migration inhibitory factor Homo sapiens 0-38 9560321-1 1998 Macrophage-migration-inhibitory factor (MIF) is retained by S-hexylglutathione-agarose but is not specifically eluted in high yield. Sepharose 79-86 macrophage migration inhibitory factor Homo sapiens 40-43 10719057-8 2000 Using anti-MIF antibody-immobilized sepharose column chromatography, we discovered for the first time that MIF was present in erythrocytes. Sepharose 36-45 macrophage migration inhibitory factor Homo sapiens 11-14 10719057-8 2000 Using anti-MIF antibody-immobilized sepharose column chromatography, we discovered for the first time that MIF was present in erythrocytes. Sepharose 36-45 macrophage migration inhibitory factor Homo sapiens 107-110 1932371-5 1991 F5 cells produced two species of MIF that could be separated on a phenyl-Sepharose column. Sepharose 73-82 macrophage migration inhibitory factor Homo sapiens 33-36 3549912-6 1987 Accurate determination of MIF became feasible by using cells from the human monocytoid cell line U937 as target cells in a microdroplet agarose assay. Sepharose 136-143 macrophage migration inhibitory factor Homo sapiens 26-29 3311040-4 1987 The OC-MIF was purified about 10,000 fold by affinity chromatography on L-fucose-Sepharose 6B. Sepharose 81-90 macrophage migration inhibitory factor Homo sapiens 7-10 3311040-9 1987 This monoclonal antibody coupled to Sepharose 4B adsorbed OC-MIF. Sepharose 36-48 macrophage migration inhibitory factor Homo sapiens 61-64 3552334-4 1986 MIF was assayed with a recently developed test system using the human monocytoid cell-line U937 as indicator cells in agarose microdroplets. Sepharose 118-125 macrophage migration inhibitory factor Homo sapiens 0-3 3518711-3 1986 A macrophage migration inhibition factor (OC-MIF) has been isolated from ascites fluid of ovarian cancer patients by affinity chromatography on L-fucose-Sepharose 6B, and characterized biochemically. Sepharose 153-162 macrophage migration inhibitory factor Homo sapiens 45-48 6225985-4 1983 MIF was assessed by the inhibition of adult mononuclear phagocyte cell migration under agarose; LIF was assessed by polymorphonuclear cell migration under agarose. Sepharose 87-94 macrophage migration inhibitory factor Homo sapiens 0-3 793749-1 1976 Using an assay of macrophage migration, where the cells emigrate from an agarose droplet, it was found that the neutral proteases trypsin, chymotrypsin, Pronase and elastase have MIF-like activity. Sepharose 73-80 macrophage migration inhibitory factor Homo sapiens 179-182 3881194-4 1985 Finally, glycolipids from HL60-TPA cells but not from HL-60 cells were able to reversibly bind MIF when covalently coupled to agarose. Sepharose 126-133 macrophage migration inhibitory factor Homo sapiens 95-98 3917277-6 1985 However, MIF activity in supernatant fluid from human peripheral blood lymphocyte cultures stimulated with Con A-Sepharose was completely neutralized with MoAb anti-IFN-gamma. Sepharose 113-122 macrophage migration inhibitory factor Homo sapiens 9-12 6358361-1 1983 A method is described for quantitating the biological activity of macrophage migration inhibitory factor (MIF) based on the detection of macrophages migrating from agarose droplets. Sepharose 164-171 macrophage migration inhibitory factor Homo sapiens 66-104 6358361-1 1983 A method is described for quantitating the biological activity of macrophage migration inhibitory factor (MIF) based on the detection of macrophages migrating from agarose droplets. Sepharose 164-171 macrophage migration inhibitory factor Homo sapiens 106-109 6345005-5 1983 This MIF species constitutes a major form secreted by Mo cells; it adheres to Con A-Sepharose, is trypsin-resistant, and is denser than pure protein as determined by CsCl density gradient centrifugation. Sepharose 84-93 macrophage migration inhibitory factor Homo sapiens 5-8 6345005-10 1983 These larger MIF forms have a pI of 3.0 to 3.5 and of 4.6 to 5.2 and lack affinity to Con A Sepharose. Sepharose 92-101 macrophage migration inhibitory factor Homo sapiens 13-16 333629-0 1977 [Advantages and technical conditions of the migration inhibition test [MIF] in agarose gel (author"s transl)]. Sepharose 79-86 macrophage migration inhibitory factor Homo sapiens 71-74 1243915-18 1975 From these observations, it is concluded that MIF against Tg can easily be detected by the agarose plate technique and that cellular immunity may play a more important role in the initial phase of autoimmune thyroid diseases than the later phase, in which the serum levels of circulating antibodies are becoming predominant. Sepharose 91-98 macrophage migration inhibitory factor Homo sapiens 46-49 4375308-0 1974 [Determination of macrophage migration inhibitory factor in the HEPES-buffered agarose medium. Sepharose 79-86 macrophage migration inhibitory factor Homo sapiens 18-56 4684036-0 1973 Migration inhibitory effect of cell-free supernatants from tuberculin-stimulated cultures of human mononuclear leukocytes demonstrated by two-step MIF agarose assay. Sepharose 151-158 macrophage migration inhibitory factor Homo sapiens 147-150 29996006-4 2018 METHODS: We analyzed the MIF-173G/C (rs755622) polymorphism in 180 ALL cases and 150 healthy control children by amplification of the gene using a polymerase chain reaction followed by restriction endonuclease digestion and running on an agarose gel for visualization of the product. Sepharose 238-245 macrophage migration inhibitory factor Homo sapiens 25-28