PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 1821803-1 1991 Pneumocystis carinii dihydrofolate reductase (DHFR) expressed in Escherichia coli was purified to homogeneity in a single step using methotrexate-Sepharose affinity chromatography. Sepharose 146-155 Dihydrofolate reductase Escherichia coli 21-44 8920005-2 1996 The DHFR domain was purified to homogeneity by methotrexate-Sepharose chromatography followed by an anion-exchange chromatography step in a mono Q column, and displayed a single 27-kDa band on SDS-PAGE. Sepharose 60-69 Dihydrofolate reductase Escherichia coli 4-8 1821803-1 1991 Pneumocystis carinii dihydrofolate reductase (DHFR) expressed in Escherichia coli was purified to homogeneity in a single step using methotrexate-Sepharose affinity chromatography. Sepharose 146-155 Dihydrofolate reductase Escherichia coli 46-50